Lcy07g019680 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g019680
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr07: 48123579 .. 48125830 (-)
RNA-Seq ExpressionLcy07g019680
SyntenyLcy07g019680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGGGTTGGGGAGAGAGAGAGGATCTTTTATTTACTTTTAATTTTGATCTGTTTGTTTTCATTTCTCTCTCTCTCTCGCTCTTTGGCGTTTGGCCTTCGCCTCGGCCTCCGTTCTGTTCCCCTTTTCCACCGAATTCGCTGTTTTCTATCGGAGCAAACGCACAGATTCCTGGAATTCCTCAACCAGATTCTCCTCTTCGCTCTGGCTTTCAAGTAATTCCTTTTCCACATCTTCTTCTTTGTTTGCGTTTCTCATTTGCTCCTCCAGATTACAATTATTTGTTTTCCTAATTACCCTTTTCCTATCTTATTCCGTTCTCTTCTACATCTTTCTCGTTACCATTTCCTCAATCTTCAATCTCTGCCTATCGTTTCCTCCAATGCCAAGGAGACTTTTTCATGATGCTGCAATCCCCCTTTTGATGGTTGACCGGGGGATCTGGGATCTGATTATTCACTAGATCTGTGCAGAATTTTCCAAGTTTTTGCGCATTCCACCTCACATCTGATGGATTTTTCTTCTTCTTCTTCTTCTTCTTTTTCCGAGATGAGCTACCTATGATTTGTGATCATCCTGTTCTCTTTCTTCCTTTGTGACAGAAAATAGAGTAGCTGAAGCTGTGGTTTCTTCCGGGGAGCATATCCTTCAAAGGGGAAAAGGTGAAACGAACCACACGTATAGGATGGACGAGTCAACTGCGACAAGATATTGGTGTCACATGTGTTCTCAGATTGTGAATCCCATCGTTGACGTTGAAATTAAATGCCCCTTCTGCCGTAGTGGCTTTGTCGAAGAGATAGGTAATGGTATTTCCAGCGACAATAATGAGCTCGAATCCGAATTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATTCTGCTTGGCATGATGGGAAACCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTCGATGAGGATGATGATGATAACGACCATGATGATGGGGAGGGGCATCGTCTGACTGAGCCTACGGAGTTGGACCGAGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAATTATGAGGGAGAAAGGAACAGAGAAAGAGAAAGAGACCGAGAGCGGGTTATTCTCATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTCAAACTGAGATCAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTTGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCTAATAGATATGGCACTCCACCAGCGCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTAAGGGTTGAGGATAGTTTGCAATGTTCGGTGTGTTTGGATGATTTTGAGGTTGATGCAGAAGCCAAGGAGATGCCGTGCAAGCATAAGTTTCACACTGGCTGCATTCTGCCGTGGCTTGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCCAAGCGTGATGTGGTGGATGGTGGTGGGTCTACTAGGTTCATGGCTGACATTAATGTTAATAATAATGGTGGTGGCAGTGGCAGTGGGGAGATTGAGGATGGGAGAAATGCTTCTGGGAGAAGGTTTTCGTTTCCATGGCCGTTCAATGGCTTGTTTTCTTCACAAGCTGGAGGTGGAAACTCCTCGGGAGGAAGTACAAGTGATTCTCAAAGGGATGAGAATTCCATTTGATTATCATATGAAGCTGATTGATATGGTGCTTCTGCTCTGCTTGCTGAATCAATCATCATCATCATCGTGTGTATGTATGTATATGTATGTATGTGTGTTATACTATACAGATGTTTTGCCTCCTACCCTTAGAAGAATCTCTCTTCTTTTTGCTATTTTACCAACATTGTATATGTCCTCTCACTCTCACCGCTGCTTCAATTGCTATTCTTTCACCTCCAATAACAAATATGCCGTTTTGTTTCCTTTTGTTTTAGTATCGCCTCAACTCAACGCAGGCTCTACTTAACGGTGTGAATTGTGATTATGCAATATATAGAGCTAAATCATCAGACTCATACCACAACCTTTCAAACCGAGAATTCCATTTGCCTAGCATTATACACAATTAGTTTTGGCATAGACATACAAGAGGTTGATGTAGTCCTCTTTCCATGTATTCAAATGTTAATAATTCAACTCAAAACTCTCTCCTGTATCATTCTGTATTC

mRNA sequence

GTCGGGTTGGGGAGAGAGAGAGGATCTTTTATTTACTTTTAATTTTGATCTGTTTGTTTTCATTTCTCTCTCTCTCTCGCTCTTTGGCGTTTGGCCTTCGCCTCGGCCTCCGTTCTGTTCCCCTTTTCCACCGAATTCGCTGTTTTCTATCGGAGCAAACGCACAGATTCCTGGAATTCCTCAACCAGATTCTCCTCTTCGCTCTGGCTTTCAAGTAATTCCTTTTCCACATCTTCTTCTTTGTTTGCGTTTCTCATTTGCTCCTCCAGATTACAATTATTTGTTTTCCTAATTACCCTTTTCCTATCTTATTCCGTTCTCTTCTACATCTTTCTCGTTACCATTTCCTCAATCTTCAATCTCTGCCTATCGTTTCCTCCAATGCCAAGGAGACTTTTTCATGATGCTGCAATCCCCCTTTTGATGGTTGACCGGGGGATCTGGGATCTGATTATTCACTAGATCTGTGCAGAATTTTCCAAGTTTTTGCGCATTCCACCTCACATCTGATGGATTTTTCTTCTTCTTCTTCTTCTTCTTTTTCCGAGATGAGCTACCTATGATTTGTGATCATCCTGTTCTCTTTCTTCCTTTGTGACAGAAAATAGAGTAGCTGAAGCTGTGGTTTCTTCCGGGGAGCATATCCTTCAAAGGGGAAAAGGTGAAACGAACCACACGTATAGGATGGACGAGTCAACTGCGACAAGATATTGGTGTCACATGTGTTCTCAGATTGTGAATCCCATCGTTGACGTTGAAATTAAATGCCCCTTCTGCCGTAGTGGCTTTGTCGAAGAGATAGGTAATGGTATTTCCAGCGACAATAATGAGCTCGAATCCGAATTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATTCTGCTTGGCATGATGGGAAACCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTCGATGAGGATGATGATGATAACGACCATGATGATGGGGAGGGGCATCGTCTGACTGAGCCTACGGAGTTGGACCGAGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAATTATGAGGGAGAAAGGAACAGAGAAAGAGAAAGAGACCGAGAGCGGGTTATTCTCATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTCAAACTGAGATCAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTTGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCTAATAGATATGGCACTCCACCAGCGCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTAAGGGTTGAGGATAGTTTGCAATGTTCGGTGTGTTTGGATGATTTTGAGGTTGATGCAGAAGCCAAGGAGATGCCGTGCAAGCATAAGTTTCACACTGGCTGCATTCTGCCGTGGCTTGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCCAAGCGTGATGTGGTGGATGGTGGTGGGTCTACTAGGTTCATGGCTGACATTAATGTTAATAATAATGGTGGTGGCAGTGGCAGTGGGGAGATTGAGGATGGGAGAAATGCTTCTGGGAGAAGGTTTTCGTTTCCATGGCCGTTCAATGGCTTGTTTTCTTCACAAGCTGGAGGTGGAAACTCCTCGGGAGGAAGTACAAGTGATTCTCAAAGGGATGAGAATTCCATTTGATTATCATATGAAGCTGATTGATATGGTGCTTCTGCTCTGCTTGCTGAATCAATCATCATCATCATCGTGTGTATGTATGTATATGTATGTATGTGTGTTATACTATACAGATGTTTTGCCTCCTACCCTTAGAAGAATCTCTCTTCTTTTTGCTATTTTACCAACATTGTATATGTCCTCTCACTCTCACCGCTGCTTCAATTGCTATTCTTTCACCTCCAATAACAAATATGCCGTTTTGTTTCCTTTTGTTTTAGTATCGCCTCAACTCAACGCAGGCTCTACTTAACGGTGTGAATTGTGATTATGCAATATATAGAGCTAAATCATCAGACTCATACCACAACCTTTCAAACCGAGAATTCCATTTGCCTAGCATTATACACAATTAGTTTTGGCATAGACATACAAGAGGTTGATGTAGTCCTCTTTCCATGTATTCAAATGTTAATAATTCAACTCAAAACTCTCTCCTGTATCATTCTGTATTC

Coding sequence (CDS)

ATGGACGAGTCAACTGCGACAAGATATTGGTGTCACATGTGTTCTCAGATTGTGAATCCCATCGTTGACGTTGAAATTAAATGCCCCTTCTGCCGTAGTGGCTTTGTCGAAGAGATAGGTAATGGTATTTCCAGCGACAATAATGAGCTCGAATCCGAATTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATTCTGCTTGGCATGATGGGAAACCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTCGATGAGGATGATGATGATAACGACCATGATGATGGGGAGGGGCATCGTCTGACTGAGCCTACGGAGTTGGACCGAGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAATTATGAGGGAGAAAGGAACAGAGAAAGAGAAAGAGACCGAGAGCGGGTTATTCTCATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTCAAACTGAGATCAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTTGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCTAATAGATATGGCACTCCACCAGCGCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTAAGGGTTGAGGATAGTTTGCAATGTTCGGTGTGTTTGGATGATTTTGAGGTTGATGCAGAAGCCAAGGAGATGCCGTGCAAGCATAAGTTTCACACTGGCTGCATTCTGCCGTGGCTTGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCCAAGCGTGATGTGGTGGATGGTGGTGGGTCTACTAGGTTCATGGCTGACATTAATGTTAATAATAATGGTGGTGGCAGTGGCAGTGGGGAGATTGAGGATGGGAGAAATGCTTCTGGGAGAAGGTTTTCGTTTCCATGGCCGTTCAATGGCTTGTTTTCTTCACAAGCTGGAGGTGGAAACTCCTCGGGAGGAAGTACAAGTGATTCTCAAAGGGATGAGAATTCCATTTGA

Protein sequence

MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNELESEFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRRRSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQGGQTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMADINVNNNGGGSGSGEIEDGRNASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDENSI
Homology
BLAST of Lcy07g019680 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 145/376 (38.56%), Postives = 194/376 (51.60%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNELESEFGSERAL 60
           M E+  TRYWCH C Q +   +  EIKCP C  GFVEE    ++ +  E  +    E   
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEE----MTDEEIERLTNRQPEPGF 60

Query: 61  SLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRRR 120
           S W PI                   D DD+DND              L RE +  IRR R
Sbjct: 61  SQWNPI------------EHPGETMDSDDEDND--------------LGREFEGFIRRHR 120

Query: 121 SSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQG-------- 180
            ++T+ ++L  I   +A + E             R+  ILIN FNQ + +QG        
Sbjct: 121 RASTLRRVLDSIHDDLADDQE-------------RDSSILINAFNQALALQGSVLDPDEG 180

Query: 181 -GQTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSL 240
            G    + N  G L +Y +G GL LLLQHLAE+DP+R GTPPA+KEAV+ALPTV++E+ +
Sbjct: 181 QGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVKIEEVV 240

Query: 241 QCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGG 300
            CSVCLDD EV ++AK+MPC+HKFH+ CILPWLELHSSCPVCR +LP++E+K        
Sbjct: 241 SCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEETK-------- 300

Query: 301 STRFMADINVNNNGG----------GSGSGEIEDGRNASGRRFSFPWPFNGLFSSQAGGG 358
                 D+N  +N G            G G + +  N        PW  N LFS++    
Sbjct: 301 ------DLNEPSNIGRVEDSHEEVRADGPGNVSESSNRPWA--IVPW-LNELFSTRE--A 314

BLAST of Lcy07g019680 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 5.0e-39
Identity = 86/196 (43.88%), Postives = 115/196 (58.67%), Query Frame = 0

Query: 169 VVQGGQTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVE 228
           V+Q   ++       ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA K A++ALP V + 
Sbjct: 154 VIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNIT 213

Query: 229 DS------LQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADES 288
            S       QC+VC+DDFE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP D  
Sbjct: 214 KSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTD-- 273

Query: 289 KRDVVDGGGSTRFMADINVNNNGGGSGSGEIEDGRNAS---GRRFSFPWPFNGLFSSQAG 348
                D     R       +    G  SG+  DG N +     R S PWPF      QA 
Sbjct: 274 -----DPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPF------QAR 333

Query: 349 GGNSSGGSTSDSQRDE 356
           G   + G  +++++++
Sbjct: 334 GPGPAPGDNAETRQED 336

BLAST of Lcy07g019680 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.5e-38
Identity = 84/194 (43.30%), Postives = 111/194 (57.22%), Query Frame = 0

Query: 169 VVQGGQTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVE 228
           V++   ++      G+ GDYF GPGL+ L+Q LAENDPNRYGTPPA K A+DALPTV+V 
Sbjct: 146 VIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVT 205

Query: 229 DSL------QCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADES 288
             +      QC+VC+D+FE  ++ K+MPCKH FH  C+LPWLELH+SCPVCR +LP D+ 
Sbjct: 206 KDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDP 265

Query: 289 KRDVVDGGGSTRFMADINVNNNGGGSGSGEIEDGRNASGRRFSFPWPFNGLFSSQAGGGN 348
             +    G            + G G G G +E G+         PWPF     S +G G 
Sbjct: 266 DYENRSQG------------SQGSGDGQGSVE-GQQTPRFSIQLPWPFRRQDGSGSGSGA 325

Query: 349 SSGGSTSDSQRDEN 357
              G  +   R E+
Sbjct: 326 PGTGGGNLETRGED 326

BLAST of Lcy07g019680 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.2e-26
Identity = 72/180 (40.00%), Postives = 97/180 (53.89%), Query Frame = 0

Query: 183 GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV------EDSLQCSVC 242
           G+ GDYF GPGL+ L + L+     R G PPA + A+DALPT+++           C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 243 LDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFM 302
            D+FE+ +EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP+        +    TR  
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTR-- 254

Query: 303 ADINVNNNGGGSGSGEIEDGRNASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 357
              N  ++   S S   E+G N     FS  WPF    SS +   N  G   SD+  DEN
Sbjct: 255 ---NYRSSSSSSSSNSRENG-NERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSDTS-DEN 306

BLAST of Lcy07g019680 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.4e-25
Identity = 103/364 (28.30%), Postives = 147/364 (40.38%), Query Frame = 0

Query: 4   STATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNELESE--FGSERALS 63
           S    +WCH C + V  +   +  C +C  GFVEEI  G S  + ++E +  F    A S
Sbjct: 3   SIRNTHWCHRCQRAV-WLRARDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFS 62

Query: 64  LWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRRRS 123
            +             R R     +D                    E+   L S      S
Sbjct: 63  AFM------------RSRLAERSYDR-------------------EISGRLGSAGSESFS 122

Query: 124 SATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQGGQTEINQNP 183
           +   L +  G         +N   E                       V G    I    
Sbjct: 123 NLAPLLIFGGQAPFRLAGGDNSSVE---------------------AFVNGAAPGIGIAR 182

Query: 184 IGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV------EDSLQCSV 243
             + GDYF GPGL+ L++ L+    +R G PPA K ++DALPT+++           C V
Sbjct: 183 GTNAGDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPV 242

Query: 244 CLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRF 303
           C D+FE+ +EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP+          G S+  
Sbjct: 243 CKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS---------RGSSSST 281

Query: 304 MADINVNNNGGGSGSGEIEDGRNASGRR--FSFPWPFNGLFSSQAGGGNSSGGSTSDSQR 358
            +  N + N           GR  S RR  FS  WPF            SS  S++ ++R
Sbjct: 303 QSSQNRSTN-----------GRENSRRRNIFSNLWPF-----------RSSSSSSTQNRR 281

BLAST of Lcy07g019680 vs. ExPASy TrEMBL
Match: A0A6J1KAR4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491659 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.1e-177
Identity = 321/362 (88.67%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 120

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 121 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 180

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 181 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 240

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 241 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 300

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+NVNNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 301 DLNVNNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 355

BLAST of Lcy07g019680 vs. ExPASy TrEMBL
Match: A0A6J1H411 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459795 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.4e-177
Identity = 320/362 (88.40%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 120

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 121 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 180

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 181 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 240

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 241 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 300

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+N+NNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 301 DLNINNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 355

BLAST of Lcy07g019680 vs. ExPASy TrEMBL
Match: A0A6J1DLY0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021272 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.9e-170
Identity = 311/379 (82.06%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNELESEFGSERAL 60
           M+ES  TRYWCHMCSQ+VNPI DVEIKCPFCRSGFVEEIGNGISSDNNELES+FGSERAL
Sbjct: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60

Query: 61  SLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHR---LTEPTELDRELDSVIR 120
           SLWAPILLGMMGN RGRRR RHLEFDED+++N++DDGEGH     TE TELDRELDS+IR
Sbjct: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120

Query: 121 RRRSSATILQLLQGIRAGMATESENYEGERN--RERERDRERVILINPFNQTIVVQGGQT 180
           RRRSSATILQLLQGIRAGMATESENYEG+RN  RERER+RERVILINPFNQTIVVQGG T
Sbjct: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT 180

Query: 181 EINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSV 240
            ++QNP+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPAQ+EAVDALPTVRVEDSLQCSV
Sbjct: 181 -VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSV 240

Query: 241 CLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGS--- 300
           CLDDFEV+AEAKEMPCKHKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+  G GS   
Sbjct: 241 CLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSA 300

Query: 301 TRFMADINVNNNGGGSGSGEIE---DGRNASGRRFSFPWPFNGLFSSQAGGG-------- 359
            RFM D+NVN    G GSG+IE   DGRNASGRRF FPWPFNGLFSSQ GGG        
Sbjct: 301 ARFMGDLNVN----GGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNS 360

BLAST of Lcy07g019680 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.5e-158
Identity = 304/377 (80.64%), Postives = 324/377 (85.94%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSD----------NNEL 60
           MDE  ATRYWCHMCSQ+VNPIVDVEIKCPFCRSGFVEEIGNGISSD          NNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESEFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDR 120
           ES+FGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN+H+DGEG+R++  T    
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSET---T 120

Query: 121 ELDSVIRRRRSSATILQLLQGIRAGMAT-ESEN-YEGERNRERERDRERVILINPFNQTI 180
           ELDSVIRRRRSSATILQLLQGIRAG+AT ESEN YEGER+RE    RERVILINPFNQTI
Sbjct: 121 ELDSVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRE----RERVILINPFNQTI 180

Query: 181 VVQGGQTE-INQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV 240
           VVQGG+ +  NQN IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDALPTVRV
Sbjct: 181 VVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV 240

Query: 241 E----DSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESK 300
           E      LQCSVCLD+FEVD EAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP DESK
Sbjct: 241 ELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESK 300

Query: 301 RDVVDGGGSTRFMADINVNNNGGGSGSGEIEDGRNA--SGRRFSFPWPFNGLFSSQAGGG 359
           RD VDGG + RFM+D+   NNG G+GSGEIE GRN+  SGRRFSFPWPFNGLF+SQ   G
Sbjct: 301 RD-VDGGSAARFMSDL---NNGNGNGSGEIE-GRNSSESGRRFSFPWPFNGLFTSQ---G 360

BLAST of Lcy07g019680 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.8e-157
Identity = 300/377 (79.58%), Postives = 322/377 (85.41%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSD----------NNEL 60
           MDE  ATRYWCHMCSQ+VNPIVDVEIKCPFCRSGFVEEIGNGISSD          NNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESEFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDR 120
           ES+FGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN+H+DGEG+ ++  T    
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSET---T 120

Query: 121 ELDSVIRRRRSSATILQLLQGIRAGMAT-ESEN-YEGERNRERERDRERVILINPFNQTI 180
           ELDSVIRRRRSSATILQLLQGIRAG+AT ESEN YEGER+RE    RERVILINPFNQTI
Sbjct: 121 ELDSVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRE----RERVILINPFNQTI 180

Query: 181 VVQGGQTE-INQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV 240
           VVQGG+ +  N N IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDALPTVRV
Sbjct: 181 VVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV 240

Query: 241 E----DSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESK 300
           E      LQCSVCLD+FEVD EAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP DESK
Sbjct: 241 ELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESK 300

Query: 301 RDVVDGGGSTRFMADI-NVNNNGGGSGSGEIEDGRNA-SGRRFSFPWPFNGLFSSQAGGG 359
           RD VDGG + RFM+D+ N N NG G+GSGEIE+  ++ SGRRFSFPWPFNGLF+SQ   G
Sbjct: 301 RD-VDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ---G 360

BLAST of Lcy07g019680 vs. NCBI nr
Match: XP_022996432.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima] >XP_022996433.1 E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima])

HSP 1 Score: 632.5 bits (1630), Expect = 2.2e-177
Identity = 321/362 (88.67%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 120

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 121 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 180

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 181 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 240

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 241 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 300

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+NVNNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 301 DLNVNNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 355

BLAST of Lcy07g019680 vs. NCBI nr
Match: KAG7035784.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 632.1 bits (1629), Expect = 2.9e-177
Identity = 320/362 (88.40%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 122 MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 181

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 182 LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 241

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 242 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 301

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 302 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 361

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 362 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 421

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+N+NNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 422 DLNINNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 476

BLAST of Lcy07g019680 vs. NCBI nr
Match: XP_022958624.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata] >XP_022958625.1 E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 632.1 bits (1629), Expect = 2.9e-177
Identity = 320/362 (88.40%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 120

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 121 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 180

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 181 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 240

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 241 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 300

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+N+NNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 301 DLNINNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 355

BLAST of Lcy07g019680 vs. NCBI nr
Match: KAG6605826.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 632.1 bits (1629), Expect = 2.9e-177
Identity = 320/362 (88.40%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG IS DNNELES+FGSERA
Sbjct: 69  MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 128

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 129 LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 188

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 189 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 248

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 249 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQCSVCL 308

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 309 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 368

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+N+NNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 369 DLNINNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 423

BLAST of Lcy07g019680 vs. NCBI nr
Match: XP_023534189.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 318/362 (87.85%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNG-ISSDNNELESEFGSERA 60
           MDES ATRYWCHMCSQ+VNPI+DVEIKCPFCRSGFVEE+ NG +S DNNELES+FGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDVSRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHDDGEGHRLTEPTELDRELDSVIRRR 120
           LSLWAPILLGMMGNPRGRRRFRHLEFDEDD DND+D+GEGHRLTEPT+LDRELDSV+RRR
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHRLTEPTDLDRELDSVMRRR 120

Query: 121 RSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQ--GGQTEI 180
           RSSATILQLLQGIRAGMATESEN+E ERNRE    RERVILINPFNQTIVVQ  GGQTE 
Sbjct: 121 RSSATILQLLQGIRAGMATESENFERERNRE----RERVILINPFNQTIVVQGGGGQTEN 180

Query: 181 NQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQCSVCL 240
             +PIGSLGDYFVGPGLDLLLQHLAENDPNR+GTPPA KEAVDALPTVRV+D+LQCSVCL
Sbjct: 181 QNHPIGSLGDYFVGPGLDLLLQHLAENDPNRHGTPPAHKEAVDALPTVRVKDNLQCSVCL 240

Query: 241 DDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGGSTRFMA 300
           DDFEVDAEAKEMPC+HKFHTGCILPWLELHSSCPVCRHQLPADESKRD VDGGGSTRFM 
Sbjct: 241 DDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRD-VDGGGSTRFMG 300

Query: 301 DINVNNNGGGSGSGEIEDGR-NASGRRFSFPWPFNGLFSSQAGGGNSSGGSTSDSQRDEN 359
           D+N+NNNGG  G GEIEDGR NAS  RFSFPWPFNGLFSSQAG GNSSGGSTSDSQRD++
Sbjct: 301 DLNINNNGG--GFGEIEDGRNNASESRFSFPWPFNGLFSSQAGAGNSSGGSTSDSQRDDS 355

BLAST of Lcy07g019680 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.9e-110
Identity = 221/393 (56.23%), Postives = 276/393 (70.23%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEI-GNGISS------DNNELESE 60
           M+E+ A RYWCHMCSQ+VNP+++ EIKCPFC+SGF+EE+ GNG         D  + E++
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNGGGGGGRGIRDVQDSETD 60

Query: 61  FGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNDHD-----DGEG--------- 120
           FG++RALSLWAPILLGMM +PR RRRFR  EF E++DDN  D     DG G         
Sbjct: 61  FGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNSNNN 120

Query: 121 ----HRLTE---PTELDRELDSVI-RRRRSSATILQLLQGIRAGMATESENYEGERNRER 180
               HR        +LDRE +S++ RRRRSS  ILQLLQGIRAG+A+E E+   + N + 
Sbjct: 121 VYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASEYES--SDNNWDN 180

Query: 181 ERDRERVILINPFNQTIVVQGGQTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGT 240
            R+R+RVI+INP+NQ++VV   Q + N   + SLGDYF+GPGLDLLLQHLAENDPNR GT
Sbjct: 181 SRERDRVIMINPYNQSLVVPSDQNQ-NHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQGT 240

Query: 241 PPAQKEAVDALPTVRVEDSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCP 300
           PPA+KEAV+ALPTV++ + LQCSVCLDDFE   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 PPARKEAVEALPTVKIMEPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCP 300

Query: 301 VCRHQLPA-----DESKRDV--VDGGGSTRFMADINVNNNGGGSGSGEIEDGRNASGRRF 358
           VCR +LP+     DE+K D   V    + R  ++ NV  N G +  G  ++ R+ +GRRF
Sbjct: 301 VCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRSGNGRRF 360

BLAST of Lcy07g019680 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 6.2e-93
Identity = 189/357 (52.94%), Postives = 238/357 (66.67%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNELESEFGSERA- 60
           M+E+  TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEE    +  D++   S+    RA 
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEE----MEDDDDHDSSDPADVRAN 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDD-----DNDHDDGEGHRLTEPTELDRELDS 120
            SLWAPIL+ +M +P  RRR + +E  ED+      +N+ DDGE        +LD +L  
Sbjct: 61  NSLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-------NDLDWQLQE 120

Query: 121 VI-RRRRSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQGG 180
           ++ RRRR SA +LQLLQGIRAG++ ESE+     N     +  RVILIN  NQTI VQ  
Sbjct: 121 ILRRRRRHSAAVLQLLQGIRAGLSVESESTGNGGN-----NPGRVILINTSNQTITVQ-N 180

Query: 181 QTEINQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEDSLQC 240
             +++  P GSLGDYF+GPG ++LLQ LAENDPNRYGTPPA+KEAV+AL TV++E++LQC
Sbjct: 181 SADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIEETLQC 240

Query: 241 SVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVV----DG 300
           SVCLDDFE+  EAK MPC HKFH+ C+LPWLELHSSCPVCR+QLPADE+K D V    D 
Sbjct: 241 SVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSVTTTSDN 300

Query: 301 GGSTRFMADIN--VNNNGGG--------SGSGEIEDGRNASGRRFSFPWPFNGLFSS 337
            GS+   A  +    N+ G             E E+  N  G  FS PWPF+ LFSS
Sbjct: 301 NGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFSTLFSS 340

BLAST of Lcy07g019680 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-92
Identity = 202/384 (52.60%), Postives = 252/384 (65.62%), Query Frame = 0

Query: 6   ATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVE----EIGNGISSDNNEL---ESEFGSER 65
           + RYWCHMCSQ+VNP++  EIKCPFC+SGFVE    EI  G SS   E+   E +FG++R
Sbjct: 2   SARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREINGGSSSSLREVQDPEIDFGTDR 61

Query: 66  ALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDD-NDHDDGEG-------------HRLTE 125
           ALSLW PILLGMM NPR RRRFR  EF  D+D+ N  DD +G             HR  +
Sbjct: 62  ALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQ 121

Query: 126 ---PTELDRELDSVI-RRRRSSATILQLLQGIRAGMATESENYEGERNRERERDRERVIL 185
                +LDRE +S++ RRRRSSATILQLLQGIRAG+A+E E+           DR+ +  
Sbjct: 122 QGREIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYES----------SDRDLL-- 181

Query: 186 INPFNQTIVVQGGQTEINQN----PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQK 245
               NQ+ VVQ G T +NQN     + ++GDYFVG  LD LL+HLA+ND  R+G+ PA+K
Sbjct: 182 ----NQSAVVQ-GSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARK 241

Query: 246 EAVDALPTVRVEDSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 305
           E VD LPTV++ +SLQCS+CLDDF+  +EAKEMPCKHKFH  CI+PWLELHSSCPVCR++
Sbjct: 242 EVVDNLPTVKISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYE 301

Query: 306 LPADESKRDVVDGGGSTRFMADINVNNNGGGSGSGEIED---GRNASGRRFSFPWPFNGL 358
           LP D+  + V      TR + +INV+N         +ED     N S RRFSFPWPF+GL
Sbjct: 302 LPPDDETK-VNPVRPRTRTL-EINVSNE-------NVEDDARNSNVSERRFSFPWPFSGL 356

BLAST of Lcy07g019680 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-92
Identity = 202/384 (52.60%), Postives = 252/384 (65.62%), Query Frame = 0

Query: 6   ATRYWCHMCSQIVNPIVDVEIKCPFCRSGFVE----EIGNGISSDNNEL---ESEFGSER 65
           + RYWCHMCSQ+VNP++  EIKCPFC+SGFVE    EI  G SS   E+   E +FG++R
Sbjct: 2   SARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREINGGSSSSLREVQDPEIDFGTDR 61

Query: 66  ALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDD-NDHDDGEG-------------HRLTE 125
           ALSLW PILLGMM NPR RRRFR  EF  D+D+ N  DD +G             HR  +
Sbjct: 62  ALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQ 121

Query: 126 ---PTELDRELDSVI-RRRRSSATILQLLQGIRAGMATESENYEGERNRERERDRERVIL 185
                +LDRE +S++ RRRRSSATILQLLQGIRAG+A+E E+           DR+ +  
Sbjct: 122 QGREIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYES----------SDRDLL-- 181

Query: 186 INPFNQTIVVQGGQTEINQN----PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQK 245
               NQ+ VVQ G T +NQN     + ++GDYFVG  LD LL+HLA+ND  R+G+ PA+K
Sbjct: 182 ----NQSAVVQ-GSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARK 241

Query: 246 EAVDALPTVRVEDSLQCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 305
           E VD LPTV++ +SLQCS+CLDDF+  +EAKEMPCKHKFH  CI+PWLELHSSCPVCR++
Sbjct: 242 EVVDNLPTVKISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYE 301

Query: 306 LPADESKRDVVDGGGSTRFMADINVNNNGGGSGSGEIED---GRNASGRRFSFPWPFNGL 358
           LP D+  + V      TR + +INV+N         +ED     N S RRFSFPWPF+GL
Sbjct: 302 LPPDDETK-VNPVRPRTRTL-EINVSNE-------NVEDDARNSNVSERRFSFPWPFSGL 356

BLAST of Lcy07g019680 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 271.9 bits (694), Expect = 7.1e-73
Identity = 157/329 (47.72%), Postives = 202/329 (61.40%), Query Frame = 0

Query: 1   MDESTATRYWCHMCSQIVNPIVDVE-IKCPFCRSGFVEEIGNGISSDNNELESEFGSERA 60
           M++++ T YWCHMCS+ V P++  E IKC FC+SGFVEE+      DNN+       + A
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNND-----DHQAA 60

Query: 61  LSLWAPILLGMMGNPRGRRRFRHLEF-----DEDDDDNDHDDGEGHRLTEPTELDRELDS 120
            SLW PIL+ MM N   +      +      DED+D++D DDG+ +   E  ++  +L+ 
Sbjct: 61  DSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE-IDITHQLEE 120

Query: 121 VIR-RRRSSATILQLLQGIRAGMATESENYEGERNRERERDRERVILINPFNQTIVVQGG 180
           + R R R S  I+ LLQGIRAG+  ESEN     N +   + E V+LIN FNQ I V   
Sbjct: 121 IRRIRTRHSTAIVNLLQGIRAGLLIESEN-----NEDNPDNSELVVLINSFNQRIRVHQD 180

Query: 181 QTEINQNPIGSLGDYFVGPGLDLLLQHLAEND-PNRYGTPPAQKEAVDALPTVRVEDS-L 240
             +    P GSLGDYF+GPG + LLQ LAEND  NRYGTPPA KEAV+AL  V++EDS L
Sbjct: 181 SVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAMVKIEDSLL 240

Query: 241 QCSVCLDDFEVDAEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVVDGGG 300
           QCSVCLDDFE+  EAKEMPCKHKFH+ C+LPWLELHSSCPVCR+ LP  +      D   
Sbjct: 241 QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTGDDDEPKTDAET 300

Query: 301 STRFMADINVNNNGGGSGSGEIEDGRNAS 321
           S     + +++N    S     +   N S
Sbjct: 301 SRNDDNNEDISNASMASNGSSPDSSSNNS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AVN21.8e-5738.56E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
P0CH305.0e-3943.88E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN72.5e-3843.30E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
O221972.2e-2640.00Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q94AK45.4e-2528.30E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1KAR41.1e-17788.67RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491659 P... [more]
A0A6J1H4111.4e-17788.40RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459795... [more]
A0A6J1DLY04.9e-17082.06RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102127... [more]
A0A0A0KWR32.5e-15880.64RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE... [more]
A0A1S3BNR72.8e-15779.58RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_022996432.12.2e-17788.67E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima] >XP_022996433.1 E3 ubi... [more]
KAG7035784.12.9e-17788.40E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022958624.12.9e-17788.40E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata] >XP_022958625.1 E3 u... [more]
KAG6605826.12.9e-17788.40E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023534189.11.4e-17687.85E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G56340.11.9e-11056.23RING/U-box superfamily protein [more]
AT1G55530.16.2e-9352.94RING/U-box superfamily protein [more]
AT4G26400.21.8e-9252.60RING/U-box superfamily protein [more]
AT4G26400.11.8e-9252.60RING/U-box superfamily protein [more]
AT3G13430.17.1e-7347.72RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 233..273
e-value: 6.2E-8
score: 42.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 232..274
e-value: 7.8E-13
score: 48.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 233..274
score: 12.312242
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 200..280
e-value: 2.3E-20
score: 74.1
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 7..39
e-value: 2.0E-11
score: 43.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 297..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..106
NoneNo IPR availablePANTHERPTHR15710:SF22RING/U-BOX SUPERFAMILY PROTEINcoord: 3..333
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..333
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 232..274
e-value: 8.74203E-22
score: 85.062
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 221..280

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g019680.1Lcy07g019680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity