Lcy07g017950 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g017950
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description3-hydroxy-3-methylglutarate-CoA lyase
LocationChr07: 46619657 .. 46625742 (-)
RNA-Seq ExpressionLcy07g017950
SyntenyLcy07g017950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCGGGACATTTCAATTTTTTTGTTTGCTTGTTTGAGAGGAACTGCAACCCATTTGGTTGCTTTTCAATTCCAAGTTCAATTCCCATATAATATTTCATCTCAGCGATTACTCTTTTCTTCTCTCCATCGCGCCAAAGTGTTCTTTCCTGATTTTCCACTCCAGGTTTGATTCTTCTCTACTCTTTCGTTTTGACCTTTTTCCTCTGTTTTTGGATTTTTTTTTTCTGGGTTTTCTCTTGCTTGTGCTTGGCTGTCCAGAAACCATTGATATTGTACCATTTACTGTTTCTTTAGTGTTCATGAATTATAATCTGTACGCTAATTGGTTTGTGTCAAGGAGTTTTGCGGACTCTTTTAATTTAGTCTTTTCTTTAAGAAATTGGGTTATGATCGCCTGCTAGTGTTATCTAACCGAGAATTTCGATCGGTTCTTTTAGTTTTTTGCACGCGCTTGTTTCGGGGTTCTTGGTTGCGCTCTACTTTTGGTTGAGTACTGTACTTGTGAATGGAGGTTTGATGAAATTTAGCTATAATTCTGGATTTAGTTTTCCTATTACTTACTTTGAGCCCTCGGTTTTCTTTCCTATGTCATCTCCTTTCTGGGTTTTGATGCTATACTGAATTTTACTCACAGTGTTAAAACTAATGCTTGGATTGTTGAGACCGCCCCTTTAATCTTGATATTTTAGGGTGCCAATGAAACTTCGATTATAAGTTATAACAGCGTTAACTTTTATGATGGAGAATTTGGTTTACAATCTATATATTGGGTCGGTAATCAGTGGCACGTCAATGGAAACTCTCCTTATATGGGGAAAATCATGAGACGTCCTTGTAATGGTGAATTTCAAGAAGCATATAATGCCACTTACTGAAATTGGCAGATGGATTTTGTATATATCAAAGATTAGAATAGTTCTTGGTGATTTTTTTGATGGCAAGAAGAACAATTCTTCATCAATGGCGCCCAAGACCAGAATGAGCTTGCCATTGGGCCATCGCCATCCCATTCATGAGTGTACTCGGTGTACAAAATACTTAGGAATAGTTGCTAGAGTTGATTGCTACTTCTGTGAATGGGATGTGTTTGTGCAACCTTGATATATCTTGGTGTATCTCTCATTACAAGACTGAGTATTGCGAGGAATGTGCCTTTAAGAATCTAATGTCAGCGTGCAATGGAAGAAATATGAAAGAGGAATTTTTAGATTTAAATTAGGAAAATAAAGCACATTTTAGAATTTGTAGCTTAACATCATCTATACTATTTAATGTGATGAACAGGTATTTAAACGGTGTAGACACAAAACTACGAGACATTGCTTTAACAATGAAGGGTAAAATCAATGGATAGTTCAATATTGTGCACCTACAATTTGCAGATGATTTAGAACATACCTGCATTTTATCTTATGTTGGAGTCCGTACCCTGTTGCTCCTAGGAGAAGTATTTGCATCTAAATCTGTTGTCTTGCTTTGCCTGCTAACTTTTGTTACATCATCAATTTAACTTCATAAGAGAAAGGAACATAACTGTACTTGACCAAAGCCAGGGAATGATTGTATTCTATTCTTGCAACGCCTTGTCTTTTTTTTCCCCTCTGAAAATATCAATACTCATCTATTTATATATTTCAAATAAATTTATCAATTGGAATTTGAGAATTTCTTTCCTGATTAGAATTTTAATACACAAATAGGTCATCCATCATATGAAATTTGATTACTATGTAATGTCATTATATATTGCTCTTCCTTACTTATATATGAGAGACTAAAAGAACAAATTTAATATCTCCTTGGTTCTTCAATCAAATGATATCACACCATTTGATAGACGAAATTTTAGATTTGACGTTTCATCTGTTACTGATTTTCTGCAAGTGAGATAGCTATACCACCCTAGACATGGCAGAAAACTTATTTGGTTCATTTCTCAAACTCAGTTCTGGTTTTTGCTTCTGCCTCCACCATGTCGAGCTTGGAGGAGCCACTTGGTCTTGACAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTTTCATCTGGTGCTTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTGCTGGATTCAAGGGGGGCGAAGTAGCTCATCCAATAGTTGCAAGTAAGCAGAATATTAATCTCTTTTTATAAGAAAATTTATCATTCTTGATTTTTTAGTTTGCTTTTGCATATTTTGCTTGTGCGATTCTTGTTGTATGGGCTGGTTTTATGACAGAAGACATCCAAACAATTTGGGGATATTGATCTTTATCAGTTATTTCTTACTAGACTTTCATGTTTGTAAGCACTCGGGCTCGCTTATTCCCAATATGACATAAATAGAATTGTGCTCCTTTTTCATCATTATTATATATATATATTAGTTTTTTTTTTTAAAAAAATCGTATCGTTGTGTGTGTGTGTTTTTTTTTCCTTTCAACAGAGAATATGATGATGATGCTAGAGAGACATTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCGCTGTCAATAGGAGGACAATGAGTGCTGGCCCAAAACATTTTGCATCTGGAGATTTTTGTGATTCACATTGCTCTCCAGGCCATGAATACCAGACTAAGAGTAGTAGCGACAATATACAGCATATGACAAATAAGGTGATTTGATACCCCATTAAGCTCGTATATCTGCAGTCACTCCCTTTGGGGATCTTGAAATAATCTACAGCAAATAGAGAGGCGTTTCCTTCCAATTTCATTTGTTTTATCTGCTTGCATCCATCCACTTGTATCTCCTTTTAATTGTTTTTCTTGCCTACCCAGTACCTATCATATTCTAGATACTTTGCAAACTTTGCAAACTCTCTTAGCGTGTGACCTTCTCTGATCTTATTTTGAATATGGGCCTGCAGTTTTTGAATGGTATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACGTCTGTAAAGGTTGAATTGATCCACAGACTAGTATCCTCAGGATTGCCTGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGGTAATTTTGGTATAAGTTCCCTTTGACATTTTTTAGCCATATTTCTAAATGATATTGAAAATAGCCGTTTGAATCTCTTTTAAGTCTGTTAATGGTATGTGCATAGTCGTGACTGTGTATTGTGATGTTCAATTGGGATATCTTTGGTAGGAACTACTTGTGGAAGCTATTTGTGTAAATATTACAGGTTAAAACACACACACACACACACAACTTACTAACTTAAAAGAAGTTACAAAGAGAGGTTCCACTAGCCTCCTAGATCACATACGCCAAAGGGAATGTTAAATTGTGTAGTCTGCACTTCTTCCTAAGAGTTTTCAACCCTTTAAGAACTTTCTTATTTCTTTCGAGCTGAATGTTCTGTACAACAGCAAAAAAATTCAGCACACGAAATGGGCACCCTTTGTCTTAAAATGGCTGGTGGCAACTGTTATCTTTTTTCTACTTTAGTGGTTTCGCCTATACTTTTTGCACTGCTTGATTGCTTTCAAGACGAACTAACGTTGCTGCAGATTTAAAATATTTTCCTTGAAACTTCTTTTCTTTACATTTAGGATTTTTAAGTTCTGATACCGACTGGTTAGGAAATAGAGTGTGGCTTCAAGTTTCTCTCTCCATGGTTCATAAAATATTAAATAAAATTCATCATGAATTTTTTAGATTTACCATACTTCTGTATCACAAGTGCTACAAATATCTGAGATATACAGAATATATATTTTTTTATCCTCTTGGTTGGTCTATGTAGAAGTACTGTTCCAGGACTTTTCTTTCCATGTAAGATTTTATGAGAACTTGAATGCTTCCTCCTGACTTATTAGCTTAATTGAATTCACAATTTCTCATTGTCTCATCCTTTGTTCAGTTATCTGATGCAACCCAAGTGATGGAAGCTGTTCGTGATTTGGAAGGTGCCAGATTGCCAGTTTTAACACCTAATTTGAAAGTGGGTCATCTTTTAAATAATGTTGAATGTTTTTTACTTGGAGAGATTGAATCCTCTATTCTAAAACTTCACCTTCTTCCAGGGCTTTCAAGCTGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCCGAATCGTTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTTTTAATCGTTACCGTGCAGTAACTTCTGCTGCAAAAGAACTTGGAATCCCTGTTCGAGGGTATGCTTCCATAAATATCTAAATTGACATTGATCTGCCTCAATGTCCACATAAGAACTTTACCGATTCAGGCATCTTCTGGGAATAATTCCTGTAATACGAATCAGTAAAGTTCTTTTTCTTTATCATTGTGGCTTGAAGTATGAATACTTCTCTTTGATTATAGAAAAAGAAACCAGTCATTCTTCATCTTATTATTATAAGGAATTTATTTGGTTTCAAATTTTGAAGGCTTATTTTCTATTGTCGTTCTCCACATTCTGTTCTTGGAGCGTTCTCTGATTGATATTAACTGGCTATGGGTTTGTATAGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAGTTCCACCGTCTAAAGTGGCTTATGTAGCAAAAGAACTTTATGAGATGGGTTGCTTCGAAATTTCTCTTGGGGACACGATTGGAGTTGCCACGCCTGGTAACAATTTAAACCTTTTGTCTCACAATCAAAATATAGATTACTCGCCCAAAAATGAAAACCATATATGTTTAGCAACCGTTGGTTTTGAAATTTACTCGCAAATTTTAAAAATAAAAAATAGAAAACAGAATTGGTTATCAAACGAACATGTTTTTTAAAAATGAAAAACAAAAGAGAACAAAATGTTTATCCAACGATTCCCTAACGGCATGTTGCATAATATATCAAGTTTTCATTACAAACATCTTACACCTGGGTTGTCTTTTGTACTCGACATGAGTGATGCAAACATATGATTTTCTTAAACATTTGTATGATGAATTTTACTTTTGCTTTCGTCTTTTTTCTCTTTTATCTTATCTTATCTTTTGTTGGCAATAGGAACTGTTGTTCCCATGCTCGAAGCTGTGATGGCTGTCGTTCCTGTTGATAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAGTAAGGCCTCTTTTGAAGCTGAGTTTTGTCCTACAAAACTTGCCTTCCAGATATAGGGATGCAAATTGCTTCACAGCCAACTTTAACCACTAACTGGTGTTTTGTTCTTTTGAACAGATGGGTATTAGCACAATAGATTCCTCCGTTGCAGGTTTGGGTGGATGTCCATATGCAAAAGGAGCTTCAGGGAATGTCGCAACTGAGGATGTGGTGTACATGCTCCATGGACTTGGTATAAAAACCAATGTAGATTTGGCAAAGGTCATGTCTGCTGGGAATTTCATCAACAAGCATTTGGGGCGGCCATCGGGTTCGAAAACCGCCATTGCCTTGAACCGAGTTGCAGCCGATGCTTCCAAGATGTAACACATCTTAGTGATACATTTACACCTTACATTGAACAAGTAAACAAGAGTTTCACTTCAAGAAACTTTCTTGTTATATGCTGTCTTATCTGATGCAGCCATTTACCGGATCTCAAATAACATAATTATCATCTTCCATTAAGTAGAAAATTTGGGTCTGAATTCTCTCCATAATTTGAAGAGGATTTAGTTGATTCTTTCTGTGTCAGTGATTTGAATCGTTGTGAGATTGTCTTGTCAGTGTATTGGGTATAAGATATATAAAAGTATTAAATAAGATTCAAGTCACAGAGTTAACCCTGTAGATGGAAATTAATTCCGAGATTCAATTTTTCTTCCTCACAAAATTAATTTTATTCAAACGTATGGTTAAAAAAAAGTTTAATTAACAAAATTGGTTTATGCGAAGACAGGTCTGAATCGGTCTTCCGAGATATAGTTTAACAGTCATTGATTTGGGTTCTTTCAGACAATTAT

mRNA sequence

ACCGGGACATTTCAATTTTTTTGTTTGCTTGTTTGAGAGGAACTGCAACCCATTTGGTTGCTTTTCAATTCCAAGTTCAATTCCCATATAATATTTCATCTCAGCGATTACTCTTTTCTTCTCTCCATCGCGCCAAAGTGTTCTTTCCTGATTTTCCACTCCAGTTCTGGTTTTTGCTTCTGCCTCCACCATGTCGAGCTTGGAGGAGCCACTTGGTCTTGACAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTTTCATCTGGTGCTTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTGCTGGATTCAAGGGGGGCGAAGTAGCTCATCCAATAGTTGCAAAGAATATGATGATGATGCTAGAGAGACATTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCGCTGTCAATAGGAGGACAATGAGTGCTGGCCCAAAACATTTTGCATCTGGAGATTTTTGTGATTCACATTGCTCTCCAGGCCATGAATACCAGACTAAGAGTAGTAGCGACAATATACAGCATATGACAAATAAGTTTTTGAATGGTATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACGTCTGTAAAGGTTGAATTGATCCACAGACTAGTATCCTCAGGATTGCCTGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAACCCAAGTGATGGAAGCTGTTCGTGATTTGGAAGGTGCCAGATTGCCAGTTTTAACACCTAATTTGAAAGGCTTTCAAGCTGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCCGAATCGTTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTTTTAATCGTTACCGTGCAGTAACTTCTGCTGCAAAAGAACTTGGAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAGTTCCACCGTCTAAAGTGGCTTATGTAGCAAAAGAACTTTATGAGATGGGTTGCTTCGAAATTTCTCTTGGGGACACGATTGGAGTTGCCACGCCTGGAACTGTTGTTCCCATGCTCGAAGCTGTGATGGCTGTCGTTCCTGTTGATAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGTATTAGCACAATAGATTCCTCCGTTGCAGGTTTGGGTGGATGTCCATATGCAAAAGGAGCTTCAGGGAATGTCGCAACTGAGGATGTGGTGTACATGCTCCATGGACTTGGTATAAAAACCAATGTAGATTTGGCAAAGGTCATGTCTGCTGGGAATTTCATCAACAAGCATTTGGGGCGGCCATCGGGTTCGAAAACCGCCATTGCCTTGAACCGAGTTGCAGCCGATGCTTCCAAGATGTAACACATCTTAGTGATACATTTACACCTTACATTGAACAAGTAAACAAGAGTTTCACTTCAAGAAACTTTCTTGTTATATGCTGTCTTATCTGATGCAGCCATTTACCGGATCTCAAATAACATAATTATCATCTTCCATTAAGTAGAAAATTTGGGTCTGAATTCTCTCCATAATTTGAAGAGGATTTAGTTGATTCTTTCTGTGTCAGTGATTTGAATCGTTGTGAGATTGTCTTGTCAGTGTATTGGGTATAAGATATATAAAAGTATTAAATAAGATTCAAGTCACAGAGTTAACCCTGTAGATGGAAATTAATTCCGAGATTCAATTTTTCTTCCTCACAAAATTAATTTTATTCAAACGTATGGTTAAAAAAAAGTTTAATTAACAAAATTGGTTTATGCGAAGACAGGTCTGAATCGGTCTTCCGAGATATAGTTTAACAGTCATTGATTTGGGTTCTTTCAGACAATTAT

Coding sequence (CDS)

ATGTCGAGCTTGGAGGAGCCACTTGGTCTTGACAAATTGCCAAGCATGAGTACAATTGATCGGATACAAAGATTTTCATCTGGTGCTTGCCGACCTATAGGAGATGACATGGGAATGGGGATCTGCTGGATTCAAGGGGGGCGAAGTAGCTCATCCAATAGTTGCAAAGAATATGATGATGATGCTAGAGAGACATTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCGCTGTCAATAGGAGGACAATGAGTGCTGGCCCAAAACATTTTGCATCTGGAGATTTTTGTGATTCACATTGCTCTCCAGGCCATGAATACCAGACTAAGAGTAGTAGCGACAATATACAGCATATGACAAATAAGTTTTTGAATGGTATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGATATGGTACCAACGTCTGTAAAGGTTGAATTGATCCACAGACTAGTATCCTCAGGATTGCCTGTTGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAACCCAAGTGATGGAAGCTGTTCGTGATTTGGAAGGTGCCAGATTGCCAGTTTTAACACCTAATTTGAAAGGCTTTCAAGCTGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCCGAATCGTTTTCAAAATCCAACATCAATTGTAGCATTGAAGAAAGTTTTAATCGTTACCGTGCAGTAACTTCTGCTGCAAAAGAACTTGGAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGAGCAGTTCCACCGTCTAAAGTGGCTTATGTAGCAAAAGAACTTTATGAGATGGGTTGCTTCGAAATTTCTCTTGGGGACACGATTGGAGTTGCCACGCCTGGAACTGTTGTTCCCATGCTCGAAGCTGTGATGGCTGTCGTTCCTGTTGATAAGCTTGCAGTCCACTTCCATGACACTTATGGGCAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGTATTAGCACAATAGATTCCTCCGTTGCAGGTTTGGGTGGATGTCCATATGCAAAAGGAGCTTCAGGGAATGTCGCAACTGAGGATGTGGTGTACATGCTCCATGGACTTGGTATAAAAACCAATGTAGATTTGGCAAAGGTCATGTCTGCTGGGAATTTCATCAACAAGCATTTGGGGCGGCCATCGGGTTCGAAAACCGCCATTGCCTTGAACCGAGTTGCAGCCGATGCTTCCAAGATGTAA

Protein sequence

MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDDDARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQHMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNINCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAIALNRVAADASKM
Homology
BLAST of Lcy07g017950 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 602.4 bits (1552), Expect = 3.9e-171
Identity = 317/436 (72.71%), Postives = 360/436 (82.57%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPL  DKLPSMST+DRIQRFSSGACRP  DD+GMG  WI+G   ++SNSC   DD
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSC--IDD 95

Query: 61  D---ARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDN 120
           D   A+E+FPWRRH +  S+   + R    AG     SG   +S      +Y T S+ + 
Sbjct: 96  DKSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENG 155

Query: 121 IQHMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFV 180
             H++NK   GIPKFVKIVEVGPRDGLQNEK++VPTSVKVELI RLVSSGLPVVEATSFV
Sbjct: 156 TSHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFV 215

Query: 181 SPTRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKS 240
           SP  VPQL+DA  VM+AV  L+GARLPVLTPNLKGFQAAV+AGAKEVA+FASASESFS S
Sbjct: 216 SPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLS 275

Query: 241 NINCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGC 300
           NINC+IEES  RYR V +AAKE  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGC
Sbjct: 276 NINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGC 335

Query: 301 FEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTID 360
           FEISLGDTIG+ TPG+VVPMLEAVMAVVP DKLAVHFHDTYGQ+L NILVSLQMGIS +D
Sbjct: 336 FEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVD 395

Query: 361 SSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSK 420
           SS+AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FI+KHLGRP+GSK
Sbjct: 396 SSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSK 455

Query: 421 TAIALN-RVAADASKM 433
            A+ALN R+ ADASK+
Sbjct: 456 AAVALNRRITADASKI 468

BLAST of Lcy07g017950 vs. ExPASy Swiss-Prot
Match: A8WG57 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgcll1 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.3e-105
Identity = 192/293 (65.53%), Postives = 235/293 (80.20%), Query Frame = 0

Query: 129 IPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDA 188
           +P +VKIVEVGPRDGLQNEK++VPT VK++LI  L  +GLPV+EATSFVS   V Q++D 
Sbjct: 39  LPVYVKIVEVGPRDGLQNEKEIVPTEVKIQLIDLLSQTGLPVIEATSFVSSKWVAQMADH 98

Query: 189 TQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNINCSIEESFN 248
           T V++ ++     R PVLTPN++GFQAAVAAGA EVAVF SASE+FS+ NINCSIEES  
Sbjct: 99  TAVLKGIKRSPDVRYPVLTPNIQGFQAAVAAGANEVAVFGSASETFSRKNINCSIEESLQ 158

Query: 249 RYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDTIGV 308
           R+  V SAAK+ GIPVRGYVSC +GCP EG V PS+V  VAK L+E+GC+E+SLGDTIGV
Sbjct: 159 RFEQVVSAAKQEGIPVRGYVSCALGCPYEGQVKPSQVTKVAKRLFELGCYEVSLGDTIGV 218

Query: 309 ATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPY 368
            T G++  ML  V+  VP   LAVH HDTYGQ+LPNIL++LQMG+S +D+SVAGLGGCP+
Sbjct: 219 GTAGSMAEMLSDVLTEVPAGALAVHCHDTYGQALPNILIALQMGVSVVDASVAGLGGCPF 278

Query: 369 AKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAIA 422
           AKGASGNV+TED++YMLHGLGI+T VDL KVM AG+FI K L R + SK + A
Sbjct: 279 AKGASGNVSTEDLLYMLHGLGIETGVDLLKVMEAGDFICKALNRKTNSKVSQA 331

BLAST of Lcy07g017950 vs. ExPASy Swiss-Prot
Match: Q8JZS7 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 3.7e-105
Identity = 190/296 (64.19%), Postives = 235/296 (79.39%), Query Frame = 0

Query: 126 LNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQL 185
           L G+P++VKIVEVGPRDGLQNEK +VPT +K+ELI++L  +GL V+E TSFVS   VPQ+
Sbjct: 41  LPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQM 100

Query: 186 SDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNINCSIEE 245
           +D  +VM  +R   G R PVLTPNL+GFQ AVAAGA E+AVF +ASESFSK NINCSIEE
Sbjct: 101 ADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEE 160

Query: 246 SFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDT 305
           S  R++ V S+A+ + IPVRGYVSC +GCP EG++ P KV  V+K LY MGC+EISLGDT
Sbjct: 161 SMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDT 220

Query: 306 IGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGG 365
           IGV TPG++  MLE+VM  +P   LAVH HDTYGQ+L NIL +LQMGI+ +DS+V+GLGG
Sbjct: 221 IGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGG 280

Query: 366 CPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAIA 422
           CPYAKGASGNVATED++YML+G+G+ T VDL KVM AG FI K + + + SK A A
Sbjct: 281 CPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQA 336

BLAST of Lcy07g017950 vs. ExPASy Swiss-Prot
Match: Q8TB92 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Homo sapiens OX=9606 GN=HMGCLL1 PE=1 SV=3)

HSP 1 Score: 379.8 bits (974), Expect = 4.1e-104
Identity = 187/302 (61.92%), Postives = 234/302 (77.48%), Query Frame = 0

Query: 120 HMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSP 179
           H     L+G+P+FVKIVEVGPRDGLQNEK +VPT +K+E I+RL  +GL V+E TSFVS 
Sbjct: 65  HWETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTDIKIEFINRLSQTGLSVIEVTSFVSS 124

Query: 180 TRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNI 239
             VPQ++D T+VM+ +    G R PVLTPNL+GF  AVAAGA E++VF +ASESFSK NI
Sbjct: 125 RWVPQMADHTEVMKGIHQYPGVRYPVLTPNLQGFHHAVAAGATEISVFGAASESFSKKNI 184

Query: 240 NCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFE 299
           NCSIEES  ++  V  +A+ + IP RGYVSC +GCP EG++ P KV  V+K LY MGC+E
Sbjct: 185 NCSIEESMGKFEEVVKSARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYE 244

Query: 300 ISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 359
           ISLGDTIGV TPG++  MLE+VM  +P   LAVH HDTYGQ+L NIL +LQMGI+ +DS+
Sbjct: 245 ISLGDTIGVGTPGSMKRMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSA 304

Query: 360 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTA 419
           V+GLGGCPYAKGASGNVATED++YML+GLG+ T V+L KVM AG+FI K + + + SK A
Sbjct: 305 VSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTGVNLYKVMEAGDFICKAVNKTTNSKVA 364

Query: 420 IA 422
            A
Sbjct: 365 QA 366

BLAST of Lcy07g017950 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 5.4e-104
Identity = 192/296 (64.86%), Postives = 232/296 (78.38%), Query Frame = 0

Query: 130 PKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAT 189
           P+ VK+VEVGPRDGLQNEK +VPT VK+ LI  L  +GLPV+EATSFVSP  VPQ++D  
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 190 QVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNINCSIEESFNR 249
           +VM+ +  L G   PVLTPNLKGFQAAVAAGAKEV++F +ASE F+K NINCSIEES  R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 250 YRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDTIGVA 309
           +  V +AA+   IPVRGYVSCV+GCP EG +  +KVA V+K++Y MGC+EISLGD IG+ 
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 310 TPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 369
           TPG++  ML AVM  VPV  LAVH HDTYGQ+L NILV+LQMG+S +D+SVAGLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 370 KGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAIALNRV 426
           +GASGNVATED+VYML+GLGI T VDL K+M  G FI   L R + SK + A  R+
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQAACRL 298

BLAST of Lcy07g017950 vs. ExPASy TrEMBL
Match: A0A6J1JBX8 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.8e-226
Identity = 398/432 (92.13%), Postives = 416/432 (96.30%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRTMS GP+   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLKGF+AAV AGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAKEL IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of Lcy07g017950 vs. ExPASy TrEMBL
Match: A0A6J1JE93 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.8e-226
Identity = 398/432 (92.13%), Postives = 416/432 (96.30%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 22  MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 81

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRTMS GP+   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 82  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 141

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 142 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 201

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLKGF+AAV AGAKEVAVFASASESFSKSNIN
Sbjct: 202 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 261

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAKEL IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 262 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 321

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 322 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 441

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 442 AFNQLAADASKM 453

BLAST of Lcy07g017950 vs. ExPASy TrEMBL
Match: A0A6J1FZ07 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449124 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 397/432 (91.90%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRT SAG +   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTSSAGLEKIVSGNFCDSHCSPGHEYKTKSSSNNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEG RLPVLTPNLKGF AAVAAGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGVRLPVLTPNLKGFGAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAK L IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKALAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of Lcy07g017950 vs. ExPASy TrEMBL
Match: A0A6J1FZ09 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449124 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 397/432 (91.90%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 22  MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 81

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRT SAG +   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 82  DARETFPWRRHRKDTSQNGSVNRRTSSAGLEKIVSGNFCDSHCSPGHEYKTKSSSNNIQH 141

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 142 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 201

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEG RLPVLTPNLKGF AAVAAGAKEVAVFASASESFSKSNIN
Sbjct: 202 RVPQLSDAAEVMEAVRNLEGVRLPVLTPNLKGFGAAVAAGAKEVAVFASASESFSKSNIN 261

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAK L IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 262 CSIEECLDRYRAVTSAAKALAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 321

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 322 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 441

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 442 AFNQLAADASKM 453

BLAST of Lcy07g017950 vs. ExPASy TrEMBL
Match: A0A0A0KEI0 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_6G429100 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.5e-223
Identity = 396/432 (91.67%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDRIQRFSS ACRPIGDDMGMGICWIQGGRSSSSNSCKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           +  +TFPWRRHRK+TSQNG VNRRT SAG K FAS +FC+SHCSPGHEY+TKS+SDNIQH
Sbjct: 61  NGGDTFPWRRHRKNTSQNGFVNRRTTSAGSKIFASENFCESHCSPGHEYKTKSTSDNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
           MTNKFL  IPKFVKIVEVGPRDGLQNEK+MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLK F+AA+AAGAKEVAVFASASESFSK+NIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKSFRAAIAAGAKEVAVFASASESFSKANIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEES +RYRAVTSAAKEL IPVRGYVSCVVGCPTEGAV PS VAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSNVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVM VVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMGVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFIN HLGRPSGSKTAI
Sbjct: 361 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINNHLGRPSGSKTAI 420

Query: 421 ALNRVAADASKM 433
           ALNRVAAD+SKM
Sbjct: 421 ALNRVAADSSKM 432

BLAST of Lcy07g017950 vs. NCBI nr
Match: XP_023512797.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023512798.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.1 bits (2060), Expect = 3.7e-227
Identity = 401/432 (92.82%), Postives = 419/432 (96.99%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRTMSAGP+   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLKGF+AAVAAGAKEVAVFASASE FSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEES +RYRAVTSAAKEL IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of Lcy07g017950 vs. NCBI nr
Match: XP_038901632.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 795.0 bits (2052), Expect = 3.1e-226
Identity = 401/432 (92.82%), Postives = 419/432 (96.99%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DAR+T+PWRRHRK+TSQNG+VNRRTMSAG K   S +FCDSHCSPGHEY+TKS+SDNIQH
Sbjct: 61  DARDTYPWRRHRKNTSQNGSVNRRTMSAGTKKVVSENFCDSHCSPGHEYKTKSTSDNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
           +TNKFL  IPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 ITNKFLKDIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME VR+LEGARLPVLTPNLKGF+AAVAAGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMETVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEES +RYRAVTSAAKEL IPVRGYVSCVVGCPTEGA+ PSKVA+VAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAISPSKVAHVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVSMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTN+DLAKV+SAGNFINKHLGRPSGSKTAI
Sbjct: 361 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNIDLAKVISAGNFINKHLGRPSGSKTAI 420

Query: 421 ALNRVAADASKM 433
           ALNRVAADASKM
Sbjct: 421 ALNRVAADASKM 432

BLAST of Lcy07g017950 vs. NCBI nr
Match: XP_038901629.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Benincasa hispida] >XP_038901630.1 hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 795.0 bits (2052), Expect = 3.1e-226
Identity = 401/432 (92.82%), Postives = 419/432 (96.99%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYD+
Sbjct: 37  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 96

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DAR+T+PWRRHRK+TSQNG+VNRRTMSAG K   S +FCDSHCSPGHEY+TKS+SDNIQH
Sbjct: 97  DARDTYPWRRHRKNTSQNGSVNRRTMSAGTKKVVSENFCDSHCSPGHEYKTKSTSDNIQH 156

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
           +TNKFL  IPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 157 ITNKFLKDIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 216

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VME VR+LEGARLPVLTPNLKGF+AAVAAGAKEVAVFASASESFSKSNIN
Sbjct: 217 RVPQLSDAAEVMETVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASESFSKSNIN 276

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEES +RYRAVTSAAKEL IPVRGYVSCVVGCPTEGA+ PSKVA+VAKELYEMGCFEI
Sbjct: 277 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAISPSKVAHVAKELYEMGCFEI 336

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 337 SLGDTIGVATPGTVVSMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 396

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTN+DLAKV+SAGNFINKHLGRPSGSKTAI
Sbjct: 397 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNIDLAKVISAGNFINKHLGRPSGSKTAI 456

Query: 421 ALNRVAADASKM 433
           ALNRVAADASKM
Sbjct: 457 ALNRVAADASKM 468

BLAST of Lcy07g017950 vs. NCBI nr
Match: XP_022985653.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 398/432 (92.13%), Postives = 416/432 (96.30%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 22  MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 81

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRTMS GP+   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 82  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 141

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 142 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 201

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLKGF+AAV AGAKEVAVFASASESFSKSNIN
Sbjct: 202 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 261

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAKEL IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 262 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 321

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 322 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 441

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 442 AFNQLAADASKM 453

BLAST of Lcy07g017950 vs. NCBI nr
Match: XP_022985654.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP_022985655.1 hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP_022985656.1 hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 398/432 (92.13%), Postives = 416/432 (96.30%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIGDDMGMGICWIQGGRSSSSN+CKEYD+
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDNIQH 120
           DARETFPWRRHRKDTSQNG+VNRRTMS GP+   SG+FCDSHCSPGHEY+TKSSS+NIQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 120

Query: 121 MTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFL  IPKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKSNIN 240
           RVPQLSDA +VMEAVR+LEGARLPVLTPNLKGF+AAV AGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 300
           CSIEE  +RYRAVTSAAKEL IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSKTAI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+ HLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 421 ALNRVAADASKM 433
           A N++AADASKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of Lcy07g017950 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 602.4 bits (1552), Expect = 2.8e-172
Identity = 317/436 (72.71%), Postives = 360/436 (82.57%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPL  DKLPSMST+DRIQRFSSGACRP  DD+GMG  WI+G   ++SNSC   DD
Sbjct: 1   MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSC--IDD 60

Query: 61  D---ARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDN 120
           D   A+E+FPWRRH +  S+   + R    AG     SG   +S      +Y T S+ + 
Sbjct: 61  DKSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENG 120

Query: 121 IQHMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFV 180
             H++NK   GIPKFVKIVEVGPRDGLQNEK++VPTSVKVELI RLVSSGLPVVEATSFV
Sbjct: 121 TSHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFV 180

Query: 181 SPTRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKS 240
           SP  VPQL+DA  VM+AV  L+GARLPVLTPNLKGFQAAV+AGAKEVA+FASASESFS S
Sbjct: 181 SPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLS 240

Query: 241 NINCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGC 300
           NINC+IEES  RYR V +AAKE  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGC
Sbjct: 241 NINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGC 300

Query: 301 FEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTID 360
           FEISLGDTIG+ TPG+VVPMLEAVMAVVP DKLAVHFHDTYGQ+L NILVSLQMGIS +D
Sbjct: 301 FEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVD 360

Query: 361 SSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSK 420
           SS+AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FI+KHLGRP+GSK
Sbjct: 361 SSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSK 420

Query: 421 TAIALN-RVAADASKM 433
            A+ALN R+ ADASK+
Sbjct: 421 AAVALNRRITADASKI 433

BLAST of Lcy07g017950 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 602.4 bits (1552), Expect = 2.8e-172
Identity = 317/436 (72.71%), Postives = 360/436 (82.57%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPL  DKLPSMST+DRIQRFSSGACRP  DD+GMG  WI+G   ++SNSC   DD
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSC--IDD 95

Query: 61  D---ARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDN 120
           D   A+E+FPWRRH +  S+   + R    AG     SG   +S      +Y T S+ + 
Sbjct: 96  DKSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENG 155

Query: 121 IQHMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFV 180
             H++NK   GIPKFVKIVEVGPRDGLQNEK++VPTSVKVELI RLVSSGLPVVEATSFV
Sbjct: 156 TSHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFV 215

Query: 181 SPTRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKS 240
           SP  VPQL+DA  VM+AV  L+GARLPVLTPNLKGFQAAV+AGAKEVA+FASASESFS S
Sbjct: 216 SPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLS 275

Query: 241 NINCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGC 300
           NINC+IEES  RYR V +AAKE  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGC
Sbjct: 276 NINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGC 335

Query: 301 FEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTID 360
           FEISLGDTIG+ TPG+VVPMLEAVMAVVP DKLAVHFHDTYGQ+L NILVSLQMGIS +D
Sbjct: 336 FEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVD 395

Query: 361 SSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINKHLGRPSGSK 420
           SS+AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FI+KHLGRP+GSK
Sbjct: 396 SSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSK 455

Query: 421 TAIALN-RVAADASKM 433
            A+ALN R+ ADASK+
Sbjct: 456 AAVALNRRITADASKI 468

BLAST of Lcy07g017950 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.6e-114
Identity = 220/318 (69.18%), Postives = 248/318 (77.99%), Query Frame = 0

Query: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDMGMGICWIQGGRSSSSNSCKEYDD 60
           MSSLEEPL  DKLPSMST+DRIQRFSSGACRP  DD+GMG  WI+G   ++SNSC   DD
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSC--IDD 95

Query: 61  D---ARETFPWRRHRKDTSQNGAVNRRTMSAGPKHFASGDFCDSHCSPGHEYQTKSSSDN 120
           D   A+E+FPWRRH +  S+   + R    AG     SG   +S      +Y T S+ + 
Sbjct: 96  DKSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENG 155

Query: 121 IQHMTNKFLNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVELIHRLVSSGLPVVEATSFV 180
             H++NK   GIPKFVKIVEVGPRDGLQNEK++VPTSVKVELI RLVSSGLPVVEATSFV
Sbjct: 156 TSHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFV 215

Query: 181 SPTRVPQLSDATQVMEAVRDLEGARLPVLTPNLKGFQAAVAAGAKEVAVFASASESFSKS 240
           SP  VPQL+DA  VM+AV  L+GARLPVLTPNLKGFQAAV+AGAKEVA+FASASESFS S
Sbjct: 216 SPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLS 275

Query: 241 NINCSIEESFNRYRAVTSAAKELGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGC 300
           NINC+IEES  RYR V +AAKE  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGC
Sbjct: 276 NINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGC 335

Query: 301 FEISLGDTIGVATPGTVV 316
           FEISLGDTIG+ TPG  V
Sbjct: 336 FEISLGDTIGIGTPGNFV 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810273.9e-17172.71Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
A8WG571.3e-10565.533-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgc... [more]
Q8JZS73.7e-10564.193-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hm... [more]
Q8TB924.1e-10461.923-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Homo sapiens OX=9606 GN=HMG... [more]
P359155.4e-10464.86Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL... [more]
Match NameE-valueIdentityDescription
A0A6J1JBX85.8e-22692.133-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11148364... [more]
A0A6J1JE935.8e-22692.133-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC11148364... [more]
A0A6J1FZ073.2e-22491.903-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449... [more]
A0A6J1FZ093.2e-22491.903-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449... [more]
A0A0A0KEI03.5e-22391.673-hydroxy-3-methylglutarate-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_6G429100... [more]
Match NameE-valueIdentityDescription
XP_023512797.13.7e-22792.82hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_... [more]
XP_038901632.13.1e-22692.82hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Benincasa hispida][more]
XP_038901629.13.1e-22692.82hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Benincasa hispida] >X... [more]
XP_022985653.11.2e-22592.13hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita maxima][more]
XP_022985654.11.2e-22592.13hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucurbita maxima] >XP... [more]
Match NameE-valueIdentityDescription
AT2G26800.12.8e-17272.71Aldolase superfamily protein [more]
AT2G26800.22.8e-17272.71Aldolase superfamily protein [more]
AT2G26800.32.6e-11469.18Aldolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 127..425
e-value: 1.4E-112
score: 377.8
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 133..406
e-value: 2.5E-57
score: 194.4
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 133..400
score: 34.785122
NoneNo IPR availablePANTHERPTHR42738:SF15HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 53..432
NoneNo IPR availableCDDcd07938DRE_TIM_HMGLcoord: 135..406
e-value: 9.64401E-174
score: 484.974
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 129..423
IPR043594HMG-CoA lyasePANTHERPTHR42738HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 53..432
IPR000138Hydroxymethylglutaryl-CoA lyase, active sitePROSITEPS01062HMG_COA_LYASEcoord: 359..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g017950.1Lcy07g017950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046951 ketone body biosynthetic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016833 oxo-acid-lyase activity