Lcy07g016060 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g016060
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr07: 44837777 .. 44838809 (+)
RNA-Seq Expression	Lcy07g016060
Synteny	Lcy07g016060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTATCCATTCATATCTACTACAAATTTTGCAAATTAATTGTAATTTGATTGGCCAAAGGCAAAGGTCAAAATAAACCTCATTTGATAAAAATACGGTTGGAACTTACCACTTAAGTCGGCACACAGCCGTTTCAAATCAAAAGCGACGTGAACGGGCATTGAGTGATGACATCTAATAAATAACGACTGGCAACGCGACATTTGCCGCGCGCTGAATGGTGCGTACTTCCCTGGGCCCGGTGGGCTTAATAGAACAACTAATACAGCTTGGTCCAATTTTCATTTTGGGCTCGTCCTTGGGCCCATACATTCATGGCCTTTTACGTAACTACCCCTTCTCAGCATAGTTATTTTACGCAATTACCCTCTCCTAAGGTCAAGAATTCCCTCATCCACTATCACAATGAATCTTTTCTTGTAAATCAAAAAGTAAACTAATTAATAAATAAAAATTATAATGGGATCCACAAGTACCAGTCGAGGACCTCGAAGATGAACATGGACGCTGCTTGGTTCGACTCTTCAAATTCTAGAGGAGTAGGATGGATTATCCATAACTTTGAAGGTTCTTTGATTGGAGCGGGAAGCATGAAGATCAACAAGAAGGAAAATATTAAAATGATGGAAGCAAAAGCCATTTTAGAAGGGATTAAGAGGGTTATAGAGAAATATAGAGATTACCCGGAGCTGATCGATCATCCACTGGTGGTGGAATCTGATGTCGTCGAGATCATCAAGCTCCTCAACCTAGAAGCCGGAGACAATTCGGAAATTTCGCTAGTTATTGATGAAATTGGAAGCCTGTCGAGGTTTGCAATAGTGGAATCTTTCGCTTACTGCCCCACGAATTGTAACTTTTTGGCGCATAATATTGTCGTGCTGCTGCTTTACAGGGCGATTTTGTGTGTTTTTAGTTTTGATCCTTCCCGGAGCTTGAGAATGGATTCCGCCCAAGATGGGTATTGTTGTAAGGGTTGGTTTTTCTCCATTTTGGAGAAAGAAGTTGTTGTACCTAACTTTCCTTTCGTTTAA

mRNA sequence

CTTATCCATTCATATCTACTACAAATTTTGCAAATTAATTGTAATTTGATTGGCCAAAGGCAAAGGTCAAAATAAACCTCATTTGATAAAAATACGGTTGGAACTTACCACTTAAGTCGGCACACAGCCGTTTCAAATCAAAAGCGACGTGAACGGGCATTGAGTGATGACATCTAATAAATAACGACTGGCAACGCGACATTTGCCGCGCGCTGAATGGTGCGTACTTCCCTGGGCCCGGTGGGCTTAATAGAACAACTAATACAGCTTGGTCCAATTTTCATTTTGGGCTCGTCCTTGGGCCCATACATTCATGGCCTTTTACGTAACTACCCCTTCTCAGCATAGTTATTTTACGCAATTACCCTCTCCTAAGGTCAAGAATTCCCTCATCCACTATCACAATGAATCTTTTCTTGTAAATCAAAAAGTAAACTAATTAATAAATAAAAATTATAATGGGATCCACAAGTACCAGTCGAGGACCTCGAAGATGAACATGGACGCTGCTTGGTTCGACTCTTCAAATTCTAGAGGAGTAGGATGGATTATCCATAACTTTGAAGGTTCTTTGATTGGAGCGGGAAGCATGAAGATCAACAAGAAGGAAAATATTAAAATGATGGAAGCAAAAGCCATTTTAGAAGGGATTAAGAGGGTTATAGAGAAATATAGAGATTACCCGGAGCTGATCGATCATCCACTGGTGGTGGAATCTGATGTCGTCGAGATCATCAAGCTCCTCAACCTAGAAGCCGGAGACAATTCGGAAATTTCGCTAGTTATTGATGAAATTGGAAGCCTGTCGAGGTTTGCAATAGTGGAATCTTTCGCTTACTGCCCCACGAATTGTAACTTTTTGGCGCATAATATTGTCGTGCTGCTGCTTTACAGGGCGATTTTGTGTGTTTTTAGTTTTGATCCTTCCCGGAGCTTGAGAATGGATTCCGCCCAAGATGGGTATTGTTGTAAGGGTTGGTTTTTCTCCATTTTGGAGAAAGAAGTTGTTGTACCTAACTTTCCTTTCGTTTAA

Coding sequence (CDS)

ATGAACATGGACGCTGCTTGGTTCGACTCTTCAAATTCTAGAGGAGTAGGATGGATTATCCATAACTTTGAAGGTTCTTTGATTGGAGCGGGAAGCATGAAGATCAACAAGAAGGAAAATATTAAAATGATGGAAGCAAAAGCCATTTTAGAAGGGATTAAGAGGGTTATAGAGAAATATAGAGATTACCCGGAGCTGATCGATCATCCACTGGTGGTGGAATCTGATGTCGTCGAGATCATCAAGCTCCTCAACCTAGAAGCCGGAGACAATTCGGAAATTTCGCTAGTTATTGATGAAATTGGAAGCCTGTCGAGGTTTGCAATAGTGGAATCTTTCGCTTACTGCCCCACGAATTGTAACTTTTTGGCGCATAATATTGTCGTGCTGCTGCTTTACAGGGCGATTTTGTGTGTTTTTAGTTTTGATCCTTCCCGGAGCTTGAGAATGGATTCCGCCCAAGATGGGTATTGTTGTAAGGGTTGGTTTTTCTCCATTTTGGAGAAAGAAGTTGTTGTACCTAACTTTCCTTTCGTTTAA

Protein sequence

MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKYRDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNCNFLAHNIVVLLLYRAILCVFSFDPSRSLRMDSAQDGYCCKGWFFSILEKEVVVPNFPFV

Homology

[Close]

BLAST of Lcy07g016060 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 5.7e-10
Identity = 41/127 (32.28%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N DA+W DS++  G+GWII +++G ++ AG+  +    N+K++EA AILEG++ +    
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
                 +  PL +E+D  E+  LLN +  D ++   V++EI +L     + +FA      
Sbjct: 141 ------VLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRET 200

Query: 121 NFLAHNI 128
           N  AH++
Sbjct: 201 NGCAHSL 201

[Close]

BLAST of Lcy07g016060 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.4e-08
Identity = 39/128 (30.47%), Postives = 71/128 (55.47%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N DAAW   +N+ G+GWI+ + +G +I A    I  + NI  +E  AI EG++ + +++
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  -RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTN 120
            R   +    P+ +ESD +E I LL+ +  D +EI  +++EI  +     + S  +    
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

Query: 121 CNFLAHNI 128
            N +AH++
Sbjct: 209 ANKVAHDL 216

[Close]

BLAST of Lcy07g016060 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.5e-07
Identity = 37/127 (29.13%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N +AAW   +N+ G+GWI+ + +G +I A    I  + NI  +E  AI EG++ + +++
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEH 148

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
                    P+ +ESD +E I LL+ +  D +EI  +++EI  + +   + S  +     
Sbjct: 149 -------CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREA 208

Query: 121 NFLAHNI 128
           N +AH +
Sbjct: 209 NKVAHGL 208

[Close]

BLAST of Lcy07g016060 vs. ExPASy TrEMBL
Match: A0A4Y1R838 (Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.7e-07
Identity = 41/127 (32.28%), Postives = 62/127 (48.82%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N D AW   +   GVGW++ +  G  I AG     +  +  M EA+A+ E +   +E  
Sbjct: 452 LNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEAVREALTWCLELG 511

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
            D        + VESD + +IK+LN E   + EI  ++ ++  LS  A    F Y P  C
Sbjct: 512 FD-------KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLSHRAHHVEFLYAPRKC 571

Query: 121 NFLAHNI 128
           N  AHN+
Sbjct: 572 NQAAHNV 571

[Close]

BLAST of Lcy07g016060 vs. ExPASy TrEMBL
Match: A0A5N5H950 (Ribonuclease H protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_039255 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.5e-06
Identity = 36/127 (28.35%), Postives = 64/127 (50.39%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N DAAW   S+  GVGW+I NF G L  AG  + N   +++++EA A+   ++   +K 
Sbjct: 35  VNCDAAWNPQSHKGGVGWVIRNFAGLLHSAGGWE-NLHRSVEIVEATALRAALQECAKK- 94

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
                   H + +E+D   I++++N E G  + +  +I +I  L++     +  Y    C
Sbjct: 95  ------DIHSIEIETDAKTIMQMINKETGTEASLEDIIHDIWKLAQTFQNATLTYAHREC 153

Query: 121 NFLAHNI 128
           N  A  +
Sbjct: 155 NHAARAV 153

[Close]

BLAST of Lcy07g016060 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-09
Identity = 41/127 (32.28%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N DA+W DS++  G+GWII +++G ++ AG+  +    N+K++EA AILEG++ +    
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
                 +  PL +E+D  E+  LLN +  D ++   V++EI +L     + +FA      
Sbjct: 141 ------VLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRET 200

Query: 121 NFLAHNI 128
           N  AH++
Sbjct: 201 NGCAHSL 201

[Close]

BLAST of Lcy07g016060 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 68.9 bits (167), Expect = 4.9e-08
Identity = 39/128 (30.47%), Postives = 71/128 (55.47%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N DAAW   +N+ G+GWI+ + +G +I A    I  + NI  +E  AI EG++ + +++
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  -RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTN 120
            R   +    P+ +ESD +E I LL+ +  D +EI  +++EI  +     + S  +    
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

Query: 121 CNFLAHNI 128
            N +AH++
Sbjct: 209 ANKVAHDL 216

[Close]

BLAST of Lcy07g016060 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 66.2 bits (160), Expect = 3.2e-07
Identity = 37/127 (29.13%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N +AAW   +N+ G+GWI+ + +G +I A    I  + NI  +E  AI EG++ + +++
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEH 148

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
                    P+ +ESD +E I LL+ +  D +EI  +++EI  + +   + S  +     
Sbjct: 149 -------CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREA 208

Query: 121 NFLAHNI 128
           N +AH +
Sbjct: 209 NKVAHGL 208

[Close]

BLAST of Lcy07g016060 vs. NCBI nr
Match: XP_015388376.1 (uncharacterized protein LOC102612209 [Citrus sinensis])

HSP 1 Score: 64.7 bits (156), Expect = 9.3e-07
Identity = 42/128 (32.81%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N+DAA   S    G+G +I N  G +I A   +++ K  +  MEA+AIL GI     + 
Sbjct: 187 VNVDAAIQMSGLKAGLGVVIGNSNGKIIAAAVKRVSYKGTVACMEAEAILFGI-----QV 246

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
               E +  P+++ESD  E+++L+    G  +EIS  ++EI          S  + P  C
Sbjct: 247 AQQAECL--PMIIESDSTEVVELVLNRKGSMTEISWTVEEIKQRLMNHNTSSIQFAPRKC 306

Query: 121 NFLAHNIV 129
           N +AH+IV
Sbjct: 307 NAIAHSIV 307

[Close]

BLAST of Lcy07g016060 vs. NCBI nr
Match: BBH00303.1 (Ribonuclease H-like superfamily protein [Prunus dulcis])

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-06
Identity = 41/127 (32.28%), Postives = 62/127 (48.82%), Query Frame = 0


Query: 1   MNMDAAWFDSSNSRGVGWIIHNFEGSLIGAGSMKINKKENIKMMEAKAILEGIKRVIEKY 60
           +N D AW   +   GVGW++ +  G  I AG     +  +  M EA+A+ E +   +E  
Sbjct: 452 LNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEAVREALTWCLELG 511

Query: 61  RDYPELIDHPLVVESDVVEIIKLLNLEAGDNSEISLVIDEIGSLSRFAIVESFAYCPTNC 120
            D        + VESD + +IK+LN E   + EI  ++ ++  LS  A    F Y P  C
Sbjct: 512 FD-------KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLSHRAHHVEFLYAPRKC 571

Query: 121 NFLAHNI 128
           N  AHN+
Sbjct: 572 NQAAHNV 571

The following BLAST results are available for this feature:

BLAST of Lcy07g016060 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy07g016060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	5.7e-10	32.28	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DSV1	2.4e-08	30.47	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	1.5e-07	29.13	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A4Y1R838	7.7e-07	32.28	Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255...	[more]
A0A5N5H950	8.5e-06	28.35	Ribonuclease H protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674...	[more]

BLAST of Lcy07g016060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.2e-09	32.28	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022156777.1	4.9e-08	30.47	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	3.2e-07	29.13	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_015388376.1	9.3e-07	32.81	uncharacterized protein LOC102612209 [Citrus sinensis]	[more]
BBH00303.1	1.6e-06	32.28	Ribonuclease H-like superfamily protein [Prunus dulcis]	[more]

BLAST of Lcy07g016060 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..126 e-value: 4.5E-11 score: 42.6
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 1..127
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..127
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 2..125 e-value: 5.24943E-12 score: 58.0944

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g016060.1	Lcy07g016060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity