Lcy07g012750 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g012750
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationChr07: 41902950 .. 41907494 (-)
RNA-Seq ExpressionLcy07g012750
SyntenyLcy07g012750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATCAATGCCACAATGGCATCTAAGAAGACAAAGGTGGATTCTTTTATTAAGATCTTCCCCATCTACCTGTGTGGACTGCAATTTCAAATGTACTAGTTGCTAACCAATTTTATTGTTTTTTTTTAAGTTATGAAATCAAGGTGGGAGACAAGGAAAAACTCTAACATGTGAGTGATTCAGAATTCGGAAGTAGTTTTTCTTTTGAGATCGACTGTAGTGGTAAGGTGATCTTTGTCTGTATATTAAAAGAAAAACTAATGGGGTTTTAAAGCTTTAAACTGGTCTTTCTTCTTCCTTCAAATCAGAAAAATGGCAAACAGGAAAGAGGAATTTTTTAATCTGCTGGTTTTGTTGAAATTTGAAAACTGCCCATTTACGTGGGTTGAACAAGACTGTTGCTTTTTGGAGTCGTGAGAGAAAAGCTTGAAAAAAAAAAAAGGGAGTTTTGTTTTTCTTTTTTTTTTTTTTTGTAGATCTAAATAAACCATCAAAAGATACCAACATTCCACCAGACTCTCTCAATCTCAATCTCAAACTCTCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTCTCGACCGCTTTTTTGGGGCTGCAAGTTTTGGTGTGATTTCTTCAATCATATCCTCTCTGTTCCTTCTCTCTTATAAATTCCCAAAGCTCAGGATTGCAATTACCTTGAAGTGGGGATTGTTATTGTTCATTACACATTTCAATCCCCACGAAGAGTCGTTGGCCTACATTTGCGGTAGAAATAGGGATCTGGGTTTTCAATAAGATCGGCTGTTTTCGCCGACATTCTTGTTCCTTGTGGTTTAATGTTCTAAAATCGCATCTGGGTATGAGCGAATCTTTGATTTTTCTTGATAGATTGCCATAAACATTGAGATTTTGAACAATCGTCCCGATTCGTGTTTTGGGTTTTCCGTTGCCGCCGAATATTGTGTCGATAACAGGAAATGATGGCGGGGAATGGGCTGTTTTACCCGATTCTCGGATTTGCATCCTGTTTGGCTTTCATTTACATGTCGTTTGGGGATTTTAAGCTTGGGGATACTAAAGCCCCCAAAATGGACTTTGTAGAGAGAAATGGAACGCAGTTCATGGTTAATGGGAAGGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCCAGAGTCAGAGCGATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGCGGCTACAATGCCCTTCAGGTTTCTCCTGGCAGGTTTAATGAGCGAGTCTTTAAGGTATTGATTTTCACTTTGATGAGCTGCATTTTCTGATACATCTGAGATTCCTGCTGTTTCTGTGATTGGTTTTTTCACTTCTTGAACTCTTGTTTGATTCTTGCTCTCCTATGCTTTGGCTCTGCGTTTGTGATTGGTTTCTTTTTCTGAGAATTAATGGCAATGTCTGTTTTAGAGAGCGATGAAGTGATTCTGCAGTGGAAGAAAGTCTCCATTGGCCAAAGAATGGAAGCTGGGTGCTTCCACCGACAGAAGAACTGTGTCGGTAGTCATAAAACCATATGGAATTGCTTTGGAAATCGCATAAATTTTCTTGGAAAAGAACAGTAGTTTTTGCCTTTATGGTTAAGATAAATAAACTAGACTGAGGACATTAAATTGTCTGTCAGTTCCCTGTTTGGTATTGGTACCTTCTTTGGCATTTAAAGTTGGATTAATGAAGTATGATTTGAATTCTAATGTGCTGTAATCCAATGAAAAAGCATTAGAACTTGTATTTCTGTAGAAGATCAAGCTGTTATTGTTTGTTTACTTGACAATATAGTTTTAAGTTCATAAATTGAGGGCTGGAATTCAAGGAAGAACCAGATAAATTACTGCTACCATCTGTCCCTCTATTTGAGAAACAAGGAAAATGACATGTATGTATAGATGTTTTCTATAATGATTTGTTCGGTTTGACGATATTTTTTATTTGCTCAAATGCCTATGTGAAGTTGTACCGTTATCAGCTCAAGTTTTAAAAGGTTCTTCTTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGAAAACATGGGATAAGGCTTCTTCTTAGCTTAGTCAACAACTTGCAAGCATATGGTGGAAAGACTCAGTATGTGAAGTGGGCATGGCAAGAGGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCAATTCGCATTTATTTTAAGAATTATATTAAGGTACATTCCATATTTATCAATAAGTTTGTCCCTTATTTTTCATAACATTGATGTTTTGGCACTTCATCCTGAAGATTTGCTATGGATTCTGAGTTTATTGGTCCAGTAGCTAAGTTGTCCTTGTAGCACAGTGTATCTATAATATAACCGTATAAGGCATCAATTCTGTGCAATGTTTTAAAAGGCTATTGAGGCTACACCTTGAGGCTCGCCTCGAGGCAAAGCTCTAACTTTTAGCCTCGAGACTTACGTCTTCTTTGGACCCTAAGAGGCTTATGCCTTTATAAAAATGCACTGAGGCTTAAAGTCCCTACGTCTCATGTAATGGTTTGAAGAATAGTATTAATGTCCCCATTCTCTAAAAATATAAAGGTTTGAGTTGTAACAATTTGAAATATTACTACTTTTACCCCTTAGGCTTTAAAAACATAGAAAATAAAAATTTATAGATAAGTCAATCCTATAAGTGATAATAGTGGTGATGTTATGGATATGCTTAATTTTTGTTTAAGTAGCAATTCAAGGGGGTAACTTTTTTATACTTTTATATAAGTATTTTGTTTAGCATTACTTGTTCACACAAATGTAGGATTTGCGTAATGTTTTTAATTAAAATTGAGGTTTACGCCTCACACCGCCTCAAGGCCTTTAGCCTTTTAAAACATTGATTCTGCGGACCTATATCTGGTTGTAATGTGCATTATATTTTGCAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTCGGATGGGAATTGATTAATGAACCACGTTGTATGACCGATGCTTCAGGCGATACTCTTCAAGTAAGTTGTAAGATTTTGATTCGTCTGACTTATGAACCAAATGAAAATTGAACCGTACTGAACCTACTGTTCTCTAGTTTGAGTCTTTTGATTTTAGCTGGGATTTTATATGCAGCTTTTCTTTCTTTAATCTTGAATTTGTGGAAGTATTTGAGTTCCCTGCGTAGCATTTATCAACCTCAACTTTGCTTATCTTCCCCCTCCCCCTCTTTATATCTTTGCATAAACTAATTTGAGGAATGAAACTGTAGGAGTGGATAGAAGAAATGTCAGCTTACATTAAATCGATCGACAAAAGGCATCTACTAACTGTCGGGTTGGAAGGATTTTATGGTCCAAATAGCCCCAAGAAGTCTACTGTGAACCCGGAAGAGTGGGCGTCGAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTCGATTTTGCCTCTGTTCATATCTACCCCGACCACTGGTAAACATTAAACCACAATTGCAAACTTTGATTGGCTGTAATTGTTCTGAGCATGCCAAGTTCAGTTGCACATATTCTGTCTTCCAGAACATTCAATTACTCTATTCTCTTATATAATGAAATTAACATTCTGTGTGGATTAGGTTTCATGATCAAGATTTTGAAGACGAACTGAAATTCGTCTCCAAGTGGATGCTCTCTCACATTGAAGATGGTGATGTAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAAACCTGAACAAGGGCTTCACCCCAGCTCAGCGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAATGATGATTTCGGTATCGTCCCGTGGGAAAGACCATCGATATATCGGCTTATCATAGAGCAATCATGCAGGTTGGCAAGAATTGGGGGAGACACCCAACAACTAAAAGCATTGAGAGATGTGTGTGCTGAGAGATGGTGAAAGCCTGACTATGATATCTTATTTGAGAGAATTCAATTTGTTCCTGCTATATTTTGCCTCTACCCTTTGCTTCTTCTGTTGATACATATTCAGAATAGATTAGTTAGTTTAACACCTATAGTATTGAATTTCAACCAGACACTATACATGAACTGGTACAAGCTGTAATGTTTTTGAGCAAATATATAAGTCATAATCTGTTTACTTTTCAAGTCCTCTCCAAAGCTGCGATGCCAACTAGAATGCAAAACTTGATCAAAATTAGTAGCCTATAATGTGAGTGTCTTGCTTCTTAGCAAACCTCTCTAGCTTCGGAGACTCGGGAGCTTGAAGTCGTGCCCACCATCGAGATCATCAGTTCATGGTAGGAGCGAGTTTGAGGATCAAGATTTTGTAAGCTATACATCGGTGTGA

mRNA sequence

AAATCAATGCCACAATGGCATCTAAGAAGACAAAGGTGGATTCTTTTATTAAGATCTTCCCCATCTACCTGTGTGGACTGCAATTTCAAATGTACTAGTTGCTAACCAATTTTATTGTTTTTTTTTAAGTTATGAAATCAAGGTGGGAGACAAGGAAAAACTCTAACATGTGAGTGATTCAGAATTCGGAAGTAGTTTTTCTTTTGAGATCGACTGTAGTGGTAAGGTGATCTTTGTCTGTATATTAAAAGAAAAACTAATGGGGTTTTAAAGCTTTAAACTGGTCTTTCTTCTTCCTTCAAATCAGAAAAATGGCAAACAGGAAAGAGGAATTTTTTAATCTGCTGGTTTTGTTGAAATTTGAAAACTGCCCATTTACGTGGGTTGAACAAGACTGTTGCTTTTTGGAGTCGTGAGAGAAAAGCTTGAAAAAAAAAAAAGGGAGTTTTGTTTTTCTTTTTTTTTTTTTTTGTAGATCTAAATAAACCATCAAAAGATACCAACATTCCACCAGACTCTCTCAATCTCAATCTCAAACTCTCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTCTCGACCGCTTTTTTGGGGCTGCAAGTTTTGGTGTGATTTCTTCAATCATATCCTCTCTGTTCCTTCTCTCTTATAAATTCCCAAAGCTCAGGATTGCAATTACCTTGAAGTGGGGATTGTTATTGTTCATTACACATTTCAATCCCCACGAAGAGTCGTTGGCCTACATTTGCGGTAGAAATAGGGATCTGGGTTTTCAATAAGATCGGCTGTTTTCGCCGACATTCTTGTTCCTTGTGGTTTAATGTTCTAAAATCGCATCTGGGTATGAGCGAATCTTTGATTTTTCTTGATAGATTGCCATAAACATTGAGATTTTGAACAATCGTCCCGATTCGTGTTTTGGGTTTTCCGTTGCCGCCGAATATTGTGTCGATAACAGGAAATGATGGCGGGGAATGGGCTGTTTTACCCGATTCTCGGATTTGCATCCTGTTTGGCTTTCATTTACATGTCGTTTGGGGATTTTAAGCTTGGGGATACTAAAGCCCCCAAAATGGACTTTGTAGAGAGAAATGGAACGCAGTTCATGGTTAATGGGAAGGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCCAGAGTCAGAGCGATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGCGGCTACAATGCCCTTCAGGTTTCTCCTGGCAGGTTTAATGAGCGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGAAAACATGGGATAAGGCTTCTTCTTAGCTTAGTCAACAACTTGCAAGCATATGGTGGAAAGACTCAGTATGTGAAGTGGGCATGGCAAGAGGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCAATTCGCATTTATTTTAAGAATTATATTAAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTCGGATGGGAATTGATTAATGAACCACGTTGTATGACCGATGCTTCAGGCGATACTCTTCAAGAGTGGATAGAAGAAATGTCAGCTTACATTAAATCGATCGACAAAAGGCATCTACTAACTGTCGGGTTGGAAGGATTTTATGGTCCAAATAGCCCCAAGAAGTCTACTGTGAACCCGGAAGAGTGGGCGTCGAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTCGATTTTGCCTCTGTTCATATCTACCCCGACCACTGGTTTCATGATCAAGATTTTGAAGACGAACTGAAATTCGTCTCCAAGTGGATGCTCTCTCACATTGAAGATGGTGATGTAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAAACCTGAACAAGGGCTTCACCCCAGCTCAGCGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAATGATGATTTCGGTATCGTCCCGTGGGAAAGACCATCGATATATCGGCTTATCATAGAGCAATCATGCAGGTTGGCAAGAATTGGGGGAGACACCCAACAACTAAAAGCATTGAGAGATGTGTGTGCTGAGAGATGGTGAAAGCCTGACTATGATATCTTATTTGAGAGAATTCAATTTGTTCCTGCTATATTTTGCCTCTACCCTTTGCTTCTTCTGTTGATACATATTCAGAATAGATTAGTTAGTTTAACACCTATAGTATTGAATTTCAACCAGACACTATACATGAACTGGTACAAGCTGTAATGTTTTTGAGCAAATATATAAGTCATAATCTGTTTACTTTTCAAGTCCTCTCCAAAGCTGCGATGCCAACTAGAATGCAAAACTTGATCAAAATTAGTAGCCTATAATGTGAGTGTCTTGCTTCTTAGCAAACCTCTCTAGCTTCGGAGACTCGGGAGCTTGAAGTCGTGCCCACCATCGAGATCATCAGTTCATGGTAGGAGCGAGTTTGAGGATCAAGATTTTGTAAGCTATACATCGGTGTGA

Coding sequence (CDS)

ATGATGGCGGGGAATGGGCTGTTTTACCCGATTCTCGGATTTGCATCCTGTTTGGCTTTCATTTACATGTCGTTTGGGGATTTTAAGCTTGGGGATACTAAAGCCCCCAAAATGGACTTTGTAGAGAGAAATGGAACGCAGTTCATGGTTAATGGGAAGGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCCAGAGTCAGAGCGATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGCGGCTACAATGCCCTTCAGGTTTCTCCTGGCAGGTTTAATGAGCGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGAAAACATGGGATAAGGCTTCTTCTTAGCTTAGTCAACAACTTGCAAGCATATGGTGGAAAGACTCAGTATGTGAAGTGGGCATGGCAAGAGGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCAATTCGCATTTATTTTAAGAATTATATTAAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTCGGATGGGAATTGATTAATGAACCACGTTGTATGACCGATGCTTCAGGCGATACTCTTCAAGAGTGGATAGAAGAAATGTCAGCTTACATTAAATCGATCGACAAAAGGCATCTACTAACTGTCGGGTTGGAAGGATTTTATGGTCCAAATAGCCCCAAGAAGTCTACTGTGAACCCGGAAGAGTGGGCGTCGAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTCGATTTTGCCTCTGTTCATATCTACCCCGACCACTGGTTTCATGATCAAGATTTTGAAGACGAACTGAAATTCGTCTCCAAGTGGATGCTCTCTCACATTGAAGATGGTGATGTAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAAACCTGAACAAGGGCTTCACCCCAGCTCAGCGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAATGATGATTTCGGTATCGTCCCGTGGGAAAGACCATCGATATATCGGCTTATCATAGAGCAATCATGCAGGTTGGCAAGAATTGGGGGAGACACCCAACAACTAAAAGCATTGAGAGATGTGTGTGCTGAGAGATGGTGA

Protein sequence

MMAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQLKALRDVCAERW
Homology
BLAST of Lcy07g012750 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.5e-202
Identity = 324/427 (75.88%), Postives = 373/427 (87.35%), Query Frame = 0

Query: 4   GNGLFYPILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSY 63
           GNG   PILGF +C+AFIY+SFGD   G     ++ FV+RNGTQF+V+GKA Y+NGWNSY
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELAFVKRNGTQFVVDGKALYVNGWNSY 65

Query: 64  WFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDH 123
           WFMDHAV ++ + RV AML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVFKALDH
Sbjct: 66  WFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALDH 125

Query: 124 VIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNY 183
           VIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQEGVGLSSSNDSFF+DPSIR YFKNY
Sbjct: 126 VIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKNY 185

Query: 184 IKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRH 243
           +  +LTRKNS+TGIEYR+DPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID +H
Sbjct: 186 LTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNKH 245

Query: 244 LLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDF 303
           LLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+ P +DFASVHIYPDHWFHDQ F
Sbjct: 246 LLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQGF 305

Query: 304 EDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSA 363
           E++LKFV KWMLSHIEDGD E+KKPV+FTEFGLSNLNK + P+QRDRFY+T++DVIYKSA
Sbjct: 306 EEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKSA 365

Query: 364 KRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQLKAL 423
           KRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G     K  
Sbjct: 366 KRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITGRHLLDKKS 425

Query: 424 RDVCAER 431
            ++C+ R
Sbjct: 426 IEMCSHR 432

BLAST of Lcy07g012750 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.2e-188
Identity = 302/420 (71.90%), Postives = 359/420 (85.48%), Query Frame = 0

Query: 11  ILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAV 70
           ILGF  C AFIY+SF D  L      K+ FV+RNGTQF+V+ K  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 71  EEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRK 130
           +E+ +  V  ML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVF+ALDHVIAE+RK
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 131 HGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 190
           H +RLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 191 KNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLE 250
           KNS+TGIEYR+DPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID +HLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 251 GFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFV 310
           GFYGPNSPK  TVNPE+WAS+LG+DF++NS    +DFASVHIYPDHWFH+Q FE++LKFV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 311 SKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 370
            KWM SHIEDG  E+KKPV+FTEFGLSN NK + P+QRD+FY+ ++DV+YKSAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 371 GSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQLKALRDVCAER 430
           G+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G  ++ K L+++C+ R
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELCSHR 430

BLAST of Lcy07g012750 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 556.6 bits (1433), Expect = 2.5e-157
Identity = 263/445 (59.10%), Postives = 331/445 (74.38%), Query Frame = 0

Query: 1   MMAGNGL-FYPILGFASCLAFIYMSFGDFKLGD-------------TKAPKMDFVERNGT 60
           M  GNGL  Y ILG ASC+A +Y S G+  L D               A  + FVER G 
Sbjct: 1   MAVGNGLILYHILGLASCIALVYFSLGEVDLRDALPSLPFSGGASRAAAASLPFVERRGK 60

Query: 61  QFMVNGKAFYINGWNSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNAL 120
           +  ++G+ FYINGWNSYW MD AVE   +PRV +M + A  MGLTVCRTWAFNDG YNAL
Sbjct: 61  RLFLDGRPFYINGWNSYWLMDLAVEPNTRPRVSSMFRTAVSMGLTVCRTWAFNDGSYNAL 120

Query: 121 QVSPGRFNERVFKALDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSS 180
           Q+SPG F+ERVFKALD V+AE+ +HG+RL+LSL NNL AYGGK QYV+WAW+EGVGL++S
Sbjct: 121 QLSPGHFDERVFKALDRVVAEASEHGVRLILSLANNLDAYGGKRQYVRWAWEEGVGLTAS 180

Query: 181 NDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQ 240
           NDSFF+DP+IR YFK Y+KT+L RKN +TG+EYRDDPTI  WEL+NEPRC +D SGDTLQ
Sbjct: 181 NDSFFFDPAIRDYFKVYLKTLLMRKNHLTGLEYRDDPTILAWELMNEPRCTSDPSGDTLQ 240

Query: 241 EWIEEMSAYIKSIDKRHLLTVGLEGFYGP-NSPKKSTVNPEEW-ASRLGSDFIRNSEIPY 300
            W+EEMSAY+KSIDK+HLLTVG EGFYGP +S +K  +NP EW  +  G+DFIRNS+I  
Sbjct: 241 RWMEEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQD 300

Query: 301 VDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFT 360
           +DFASVH+YPD+W      +++LKF+++W+ +H+EDGD E++KPV+ TEFGLS+  +GF 
Sbjct: 301 IDFASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFE 360

Query: 361 PAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLII 420
            A RD  Y+ VYD+++ SA+R  +  G+L WQ   EGMEE +D F IVP ERPS+ RLI 
Sbjct: 361 DAHRDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIK 420

Query: 421 EQSCRLARIG-GDTQQLKALRDVCA 429
           EQSCRLA +  G+    K L+ VCA
Sbjct: 421 EQSCRLAAVRYGEEGARKVLKTVCA 445

BLAST of Lcy07g012750 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 415.2 bits (1066), Expect = 8.8e-115
Identity = 205/428 (47.90%), Postives = 282/428 (65.89%), Query Frame = 0

Query: 9   YPILGFASCLAFIYMSF-------------GDFKLG-DTKAPKMDFVERNGTQFM--VNG 68
           Y +LG    LA +Y+++             G  KL      P+M F  R GT F+    G
Sbjct: 8   YSLLGLLLLLAVVYLTWFPTTHDGGGGGGGGWVKLPVPWLQPRMPFAARRGTHFVDADTG 67

Query: 69  KAFYINGWNSYWFMDHAVEEYRKPRVRA-MLQAARKMGLTVCRTWAFNDGGYNALQVSPG 128
              Y+NGWNSYW +       R P + A ML+  R+MGL+VCRTWAF+DGG  ALQ+SPG
Sbjct: 68  SPLYVNGWNSYWLL-----PARSPALAAEMLRRGRRMGLSVCRTWAFSDGGPGALQISPG 127

Query: 129 RFNERVFKALDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFF 188
           RF+E VF+ LD+VI E+R++ IRL+L LVNNL   GGK QYV+WA   G  +++S DSF+
Sbjct: 128 RFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSFY 187

Query: 189 YDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEE 248
             P+I+ Y+K+Y+K +LTR+NS + I Y D+P IF WEL+NEPRC++++SG  LQ WI E
Sbjct: 188 SHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIAE 247

Query: 249 MSAYIKSIDKRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVH 308
           M+AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDFI+NS + ++DFASVH
Sbjct: 248 MAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVH 307

Query: 309 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGL---SNLNKGFTPAQR 368
            YPD W      E++++++S W+ SH+ D +  +KKPV+FTE G    S+ N   T   R
Sbjct: 308 AYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VDR 367

Query: 369 DRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSC 417
           D   + VYD IY SA++ ++G+G+L WQ +VEG     D+F +V  +RPS Y LI  QSC
Sbjct: 368 DIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQSC 427

BLAST of Lcy07g012750 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.0e-108
Identity = 181/393 (46.06%), Postives = 256/393 (65.14%), Query Frame = 0

Query: 21  IYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAVEEYRKPRVRA 80
           I +    ++LG    P   FV   G QF +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 81  MLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRKHGIRLLLSLV 140
             Q A + GLTV RTWAF+DGGY ALQ SPG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 141 NNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYR 200
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 201 DDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLEGFYGPNSPKK 260
           DDPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 261 STVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIED 320
            T+NP     + G+DFI N+ IP +DF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 321 GDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVE 380
               + KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 381 GMEESNDDFGIVPWERPSIYRLIIEQSCRLARI 414
           G++   D +GI+  +  S   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

BLAST of Lcy07g012750 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.2e-245
Identity = 404/431 (93.74%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGDTQQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.2e-245
Identity = 404/431 (93.74%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGDTQQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.1e-244
Identity = 402/431 (93.27%), Postives = 421/431 (97.68%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNY+KTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI +VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFTPAQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGDTQQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. ExPASy TrEMBL
Match: A0A5A7V836 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003960 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.8e-244
Identity = 402/431 (93.27%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQF++NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFILNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLA+IGGDTQQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLAKIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. ExPASy TrEMBL
Match: A0A6J1CF50 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111011126 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.1e-244
Identity = 401/427 (93.91%), Postives = 417/427 (97.66%), Query Frame = 0

Query: 1   MMAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGW 60
           MMAGNGLFYPILGFASCLAFIY+SFG+ KLG ++AP +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MMAGNGLFYPILGFASCLAFIYLSFGELKLGYSEAPIIDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAE+R+HGIRLLL LVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAEARQHGIRLLLCLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240

Query: 241 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           K+HLLTVGLEGFYGP SPKKSTVNPEEWASRLGSDF+RNS IP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPTSPKKSTVNPEEWASRLGSDFVRNSAIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHI DGD+EMKKPVMFTEFGLSNLN GFTP QRDRFYKTVYDVIY
Sbjct: 301 QDFEDELRFVSKWMLSHIADGDIEMKKPVMFTEFGLSNLNIGFTPEQRDRFYKTVYDVIY 360

Query: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 420
           KSAK+KRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLAR+GGDTQQL
Sbjct: 361 KSAKKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARVGGDTQQL 420

Query: 421 KALRDVC 428
           KAL+ VC
Sbjct: 421 KALKYVC 427

BLAST of Lcy07g012750 vs. NCBI nr
Match: XP_038902035.1 (mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida])

HSP 1 Score: 862.1 bits (2226), Expect = 2.1e-246
Identity = 407/431 (94.43%), Postives = 423/431 (98.14%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGDF++GDTK  P ++FVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDFRIGDTKVIPIVNFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDHAVEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPNVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD+EMKKPVMFTEFGLS+LNKGFTPAQRDRFYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERQSIYQLIIEQSCRLARIGGDAQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. NCBI nr
Match: XP_008448293.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 856.7 bits (2212), Expect = 8.8e-245
Identity = 404/431 (93.74%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGDTQQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. NCBI nr
Match: XP_004139946.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical protein Csa_021081 [Cucumis sativus])

HSP 1 Score: 854.4 bits (2206), Expect = 4.4e-244
Identity = 402/431 (93.27%), Postives = 421/431 (97.68%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNY+KTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI +VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFTPAQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLARIGGDTQQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. NCBI nr
Match: KAA0062055.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 854.0 bits (2205), Expect = 5.7e-244
Identity = 402/431 (93.27%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 2   MAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKA-PKMDFVERNGTQFMVNGKAFYINGW 61
           MAGNGLFYPILGFASCLAFIY+SFGD ++GDTK  P +DFVERNGTQF++NGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFILNGKAFYINGW 60

Query: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121
           NSYWFMDH+VEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 122 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181
           LDHVIAESR+HGIRLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 242 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 301
           K+HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 302 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 361
           QDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLS+LNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 362 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 421
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIY+LIIEQSCRLA+IGGDTQQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLAKIGGDTQQL 420

Query: 422 KALRDVCAERW 432
           KAL+ VCA+RW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lcy07g012750 vs. NCBI nr
Match: XP_022140455.1 (mannan endo-1,4-beta-mannosidase 2-like [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 401/427 (93.91%), Postives = 417/427 (97.66%), Query Frame = 0

Query: 1   MMAGNGLFYPILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGW 60
           MMAGNGLFYPILGFASCLAFIY+SFG+ KLG ++AP +DFVERNGTQFM+NGKAFYINGW
Sbjct: 1   MMAGNGLFYPILGFASCLAFIYLSFGELKLGYSEAPIIDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAE+R+HGIRLLL LVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAEARQHGIRLLLCLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240

Query: 241 KRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           K+HLLTVGLEGFYGP SPKKSTVNPEEWASRLGSDF+RNS IP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPTSPKKSTVNPEEWASRLGSDFVRNSAIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHI DGD+EMKKPVMFTEFGLSNLN GFTP QRDRFYKTVYDVIY
Sbjct: 301 QDFEDELRFVSKWMLSHIADGDIEMKKPVMFTEFGLSNLNIGFTPEQRDRFYKTVYDVIY 360

Query: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQL 420
           KSAK+KRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLAR+GGDTQQL
Sbjct: 361 KSAKKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARVGGDTQQL 420

Query: 421 KALRDVC 428
           KAL+ VC
Sbjct: 421 KALKYVC 427

BLAST of Lcy07g012750 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 706.8 bits (1823), Expect = 1.0e-203
Identity = 324/427 (75.88%), Postives = 373/427 (87.35%), Query Frame = 0

Query: 4   GNGLFYPILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSY 63
           GNG   PILGF +C+AFIY+SFGD   G     ++ FV+RNGTQF+V+GKA Y+NGWNSY
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELAFVKRNGTQFVVDGKALYVNGWNSY 65

Query: 64  WFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDH 123
           WFMDHAV ++ + RV AML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVFKALDH
Sbjct: 66  WFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALDH 125

Query: 124 VIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNY 183
           VIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQEGVGLSSSNDSFF+DPSIR YFKNY
Sbjct: 126 VIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKNY 185

Query: 184 IKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRH 243
           +  +LTRKNS+TGIEYR+DPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID +H
Sbjct: 186 LTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNKH 245

Query: 244 LLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDF 303
           LLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+ P +DFASVHIYPDHWFHDQ F
Sbjct: 246 LLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQGF 305

Query: 304 EDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSA 363
           E++LKFV KWMLSHIEDGD E+KKPV+FTEFGLSNLNK + P+QRDRFY+T++DVIYKSA
Sbjct: 306 EEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKSA 365

Query: 364 KRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQLKAL 423
           KRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G     K  
Sbjct: 366 KRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITGRHLLDKKS 425

Query: 424 RDVCAER 431
            ++C+ R
Sbjct: 426 IEMCSHR 432

BLAST of Lcy07g012750 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 660.6 bits (1703), Expect = 8.6e-190
Identity = 302/420 (71.90%), Postives = 359/420 (85.48%), Query Frame = 0

Query: 11  ILGFASCLAFIYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAV 70
           ILGF  C AFIY+SF D  L      K+ FV+RNGTQF+V+ K  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 71  EEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRK 130
           +E+ +  V  ML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVF+ALDHVIAE+RK
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 131 HGIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 190
           H +RLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 191 KNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLE 250
           KNS+TGIEYR+DPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID +HLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 251 GFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFV 310
           GFYGPNSPK  TVNPE+WAS+LG+DF++NS    +DFASVHIYPDHWFH+Q FE++LKFV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 311 SKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 370
            KWM SHIEDG  E+KKPV+FTEFGLSN NK + P+QRD+FY+ ++DV+YKSAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 371 GSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGGDTQQLKALRDVCAER 430
           G+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G  ++ K L+++C+ R
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELCSHR 430

BLAST of Lcy07g012750 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 5.7e-109
Identity = 181/393 (46.06%), Postives = 256/393 (65.14%), Query Frame = 0

Query: 21  IYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAVEEYRKPRVRA 80
           I +    ++LG    P   FV   G QF +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 81  MLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRKHGIRLLLSLV 140
             Q A + GLTV RTWAF+DGGY ALQ SPG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 141 NNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYR 200
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 201 DDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLEGFYGPNSPKK 260
           DDPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 261 STVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIED 320
            T+NP     + G+DFI N+ IP +DF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 321 GDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVE 380
               + KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 381 GMEESNDDFGIVPWERPSIYRLIIEQSCRLARI 414
           G++   D +GI+  +  S   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

BLAST of Lcy07g012750 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 9.7e-109
Identity = 177/373 (47.45%), Postives = 259/373 (69.44%), Query Frame = 0

Query: 39  DFVERNGTQFMVNGKAFYINGWNSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAF 98
           + V+R G QF +NG+ FY+NG+N+YW M  A +   + +V  + Q A  +G+TV RTWAF
Sbjct: 46  EMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAF 105

Query: 99  NDGGYNALQVSPGRFNERVFKALDHVIAESRKHGIRLLLSLVNNLQAYGGKTQYVKWAWQ 158
           NDG + ALQ SP  ++E VFKALD V++E+RK+ IRL+LSLVNN  AYGGK QYVKW   
Sbjct: 106 NDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNA 165

Query: 159 EGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMT 218
            G+ L +S+D FF +P++R +++++++TVL R N+ T I Y++DPTIF WEL+NEPRC +
Sbjct: 166 SGLNL-TSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPS 225

Query: 219 DASGDTLQEWIEEMSAYIKSIDKRHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIR 278
           D SGD LQ WI+EM+ ++KS+D +HL+ +GLEGFYGP++P ++  NP  +A+++G+DFIR
Sbjct: 226 DPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIR 285

Query: 279 NSEIPYVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDVEMKKPVMFTEFGLSN 338
           N+++  +DFASVH+YPD W         L+F S WM +H+ED ++ +  PV+FTEFG+S 
Sbjct: 286 NNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSA 345

Query: 339 LNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPS 398
            + GF  + RD    TVY +   S ++  +GAGSL WQ   +G E  +D + +      +
Sbjct: 346 HDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHT 405

Query: 399 IYRLIIEQSCRLA 412
             ++I  QS RLA
Sbjct: 406 ASKIISLQSKRLA 417

BLAST of Lcy07g012750 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 364.8 bits (935), Expect = 9.7e-101
Identity = 172/405 (42.47%), Postives = 255/405 (62.96%), Query Frame = 0

Query: 17  CLAF-----IYMSFGDFKLGDTKAPKMDFVERNGTQFMVNGKAFYINGWNSYWFMDHAVE 76
           CL F     I ++     LG   A    FV R G QF++NGK FY NG+N+YW    A +
Sbjct: 3   CLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATD 62

Query: 77  EYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRKH 136
              + ++  + Q A    LT+ RTW F DGGY ALQ++PG ++E+ F+ LD  IAE+++ 
Sbjct: 63  STTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRL 122

Query: 137 GIRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRK 196
           GI+++++ VNN   +GG+ QYV WA   G  + SS+D F+ +P ++ Y+KN++KT++ R 
Sbjct: 123 GIKMIITFVNNYSDFGGRKQYVDWAKNTGQNV-SSDDDFYTNPLVKQYYKNHVKTMVNRV 182

Query: 197 NSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKRHLLTVGLEG 256
           N+ T +EY+D+PTI GWEL+NEP+C  D SG TL  W+ EM+ Y+KS+D +HLL+ GLEG
Sbjct: 183 NTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEG 242

Query: 257 FYGPNSP-KKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELKFV 316
           FYG +SP +K+++NP   A+ LG+DFI N ++  +DFAS+H YPD WF + D +  L  +
Sbjct: 243 FYGDSSPQRKTSLNPVA-ANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 302

Query: 317 SKWMLSHIEDGDVEMKKPVMFTEFGLSNLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 376
            KW+  H+ED    +KKP++  EFG      G+T AQRD  +   +D IY+SA++    A
Sbjct: 303 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAA 362

Query: 377 GSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARIGG 416
           G+L W  + +GM    D   IV  E  +   +I E+S +L  I G
Sbjct: 363 GALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIRG 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2231.5e-20275.88Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ... [more]
Q9M0H61.2e-18871.90Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ... [more]
Q0JJD42.5e-15759.10Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q0DCM58.8e-11547.90Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9FJZ38.0e-10846.06Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3C3G04.2e-24593.74Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BJC24.2e-24593.74Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV... [more]
A0A0A0KB352.1e-24493.27Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3... [more]
A0A5A7V8362.8e-24493.27Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1CF506.1e-24493.91Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111011126 ... [more]
Match NameE-valueIdentityDescription
XP_038902035.12.1e-24694.43mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida][more]
XP_008448293.18.8e-24593.74PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan ... [more]
XP_004139946.14.4e-24493.27mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical pr... [more]
KAA0062055.15.7e-24493.27mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa][more]
XP_022140455.11.3e-24393.91mannan endo-1,4-beta-mannosidase 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G20680.11.0e-20375.88Glycosyl hydrolase superfamily protein [more]
AT4G28320.18.6e-19071.90Glycosyl hydrolase superfamily protein [more]
AT5G66460.15.7e-10946.06Glycosyl hydrolase superfamily protein [more]
AT5G01930.19.7e-10947.45Glycosyl hydrolase superfamily protein [more]
AT3G10890.19.7e-10142.47Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 52..352
e-value: 5.1E-14
score: 52.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 36..414
e-value: 5.2E-135
score: 452.2
NoneNo IPR availablePANTHERPTHR31451:SF50MANNAN ENDO-1,4-BETA-MANNOSIDASE 2-LIKEcoord: 1..430
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 1..430
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 39..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g012750.1Lcy07g012750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds