Lcy07g012730 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g012730
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	riboflavin synthase-like
Location	Chr07: 41882484 .. 41883323 (-)
RNA-Seq Expression	Lcy07g012730
Synteny	Lcy07g012730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGACTTTTTCTTCGCTCTCTACTGCCACCACACTATGCAATATCCCGAAAATGGTTCCTCCGGGTGGGAATACGATCCTCAATGTTCATCCAGTATCGTCTCAGTTTTGCCCTAAGTTCAAATTCAATTCCAACAGATCTCTTTTCGCTCTATTCACCTCCAGCCACAAGCCCCAGTTACGGTGCAAATCTCGGCCGAGGAGTTCCATCCAATGCCTGTTCACTGGCATTGTTGAAGAAATAGGCCGTGTCAAAAATTTGGGTATTGCCGAACATGGGGGGTTTGATTTGGAGATAACTGCGAAAACTGTTCTTGAAGGTGTGAACCTCGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACTGTGACTGCTTTTGATCACGAACTATCAGAATTCAAGGTGGGGTTGGCGCCTGAGACGCTCAGGAAGACTTCATTGGTGGAACTTGCGCCCGGTTCGCCTGTGAATTTGGAGAGGGCGGTTCAGCCAATCAGCAGAATGGGTGGGCATTTTGTGCAGGGGCATGTAGATGGGACTGGGGAGATAATCTCAATGGATCCTGAGGGGGACTCGTTGTGGATAAAAGTGAAGACATCAGAGGCTTTGCTCGATTACATTGTACCAAAAGGATTCATTGCCGTGGATGGAACAAGCTTGACTGTGGTGGATGTTTTCGACGAGGAGAAGGCTTTTAACTTTATGCTGGTTGCTTATACTCAGAAAGCCGTGGTCATTCCATTGAAGAAAGTTGGGCAGTTGGTGAATTTGGAGGTAGATATTCTTGGCAAGTATGTGCAGAGGCTCCTCAGCAATCGTGCCATCAATTCTCAGTGA

mRNA sequence

ATGTCGACTTTTTCTTCGCTCTCTACTGCCACCACACTATGCAATATCCCGAAAATGGTTCCTCCGGGTGGGAATACGATCCTCAATGTTCATCCAGTATCGTCTCAGTTTTGCCCTAAGTTCAAATTCAATTCCAACAGATCTCTTTTCGCTCTATTCACCTCCAGCCACAAGCCCCAGTTACGGTGCAAATCTCGGCCGAGGAGTTCCATCCAATGCCTGTTCACTGGCATTGTTGAAGAAATAGGCCGTGTCAAAAATTTGGGTATTGCCGAACATGGGGGGTTTGATTTGGAGATAACTGCGAAAACTGTTCTTGAAGGTGTGAACCTCGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACTGTGACTGCTTTTGATCACGAACTATCAGAATTCAAGGTGGGGTTGGCGCCTGAGACGCTCAGGAAGACTTCATTGGTGGAACTTGCGCCCGGTTCGCCTGTGAATTTGGAGAGGGCGGTTCAGCCAATCAGCAGAATGGGTGGGCATTTTGTGCAGGGGCATGTAGATGGGACTGGGGAGATAATCTCAATGGATCCTGAGGGGGACTCGTTGTGGATAAAAGTGAAGACATCAGAGGCTTTGCTCGATTACATTGTACCAAAAGGATTCATTGCCGTGGATGGAACAAGCTTGACTGTGGTGGATGTTTTCGACGAGGAGAAGGCTTTTAACTTTATGCTGGTTGCTTATACTCAGAAAGCCGTGGTCATTCCATTGAAGAAAGTTGGGCAGTTGGTGAATTTGGAGGTAGATATTCTTGGCAAGTATGTGCAGAGGCTCCTCAGCAATCGTGCCATCAATTCTCAGTGA

Coding sequence (CDS)

ATGTCGACTTTTTCTTCGCTCTCTACTGCCACCACACTATGCAATATCCCGAAAATGGTTCCTCCGGGTGGGAATACGATCCTCAATGTTCATCCAGTATCGTCTCAGTTTTGCCCTAAGTTCAAATTCAATTCCAACAGATCTCTTTTCGCTCTATTCACCTCCAGCCACAAGCCCCAGTTACGGTGCAAATCTCGGCCGAGGAGTTCCATCCAATGCCTGTTCACTGGCATTGTTGAAGAAATAGGCCGTGTCAAAAATTTGGGTATTGCCGAACATGGGGGGTTTGATTTGGAGATAACTGCGAAAACTGTTCTTGAAGGTGTGAACCTCGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACTGTGACTGCTTTTGATCACGAACTATCAGAATTCAAGGTGGGGTTGGCGCCTGAGACGCTCAGGAAGACTTCATTGGTGGAACTTGCGCCCGGTTCGCCTGTGAATTTGGAGAGGGCGGTTCAGCCAATCAGCAGAATGGGTGGGCATTTTGTGCAGGGGCATGTAGATGGGACTGGGGAGATAATCTCAATGGATCCTGAGGGGGACTCGTTGTGGATAAAAGTGAAGACATCAGAGGCTTTGCTCGATTACATTGTACCAAAAGGATTCATTGCCGTGGATGGAACAAGCTTGACTGTGGTGGATGTTTTCGACGAGGAGAAGGCTTTTAACTTTATGCTGGTTGCTTATACTCAGAAAGCCGTGGTCATTCCATTGAAGAAAGTTGGGCAGTTGGTGAATTTGGAGGTAGATATTCTTGGCAAGTATGTGCAGAGGCTCCTCAGCAATCGTGCCATCAATTCTCAGTGA

Protein sequence

MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQLRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGTCLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAINSQ

Homology

[Close]

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot
Match: Q9Y7P0 (Riboflavin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rib5 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 98/199 (49.25%), Postives = 136/199 (68.34%), Query Frame = 0


Query: 74  LFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGTCLTVTAFDHELSE 133
           +FTG+VE IG VK++      GF ++I A  +L+  + GDSIAVNGTCLTVT FD     
Sbjct: 1   MFTGLVEAIGVVKDVQGTIDNGFAMKIEAPQILDDCHTGDSIAVNGTCLTVTDFDR--YH 60

Query: 134 FKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISMDPEGDS 193
           F VG+APE+LR T+L +   G PVNLERAV   +RMGGHFVQGHVD   EI+    +G++
Sbjct: 61  FTVGIAPESLRLTNLGQCKAGDPVNLERAVLSSTRMGGHFVQGHVDTVAEIVEKKQDGEA 120

Query: 194 LWIKVKTSEA-LLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQKAVVIPLKKV 253
           +    +  +  +L YIV KG+IA+DGTSLT+  V  ++  F+ M+++YTQ  V++  K V
Sbjct: 121 IDFTFRPRDPFVLKYIVYKGYIALDGTSLTITHV--DDSTFSIMMISYTQSKVIMAKKNV 180

Query: 254 GQLVNLEVDILGKYVQRLL 272
           G LVN+EVD +GKY ++L+
Sbjct: 181 GDLVNVEVDQIGKYTEKLV 195

[Close]

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot
Match: A5DB51 (Riboflavin synthase OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=RIB5 PE=2 SV=3)

HSP 1 Score: 183.3 bits (464), Expect = 3.7e-45
Identity = 100/203 (49.26%), Postives = 133/203 (65.52%), Query Frame = 0


Query: 74  LFTGIVEEIGRV-----KNLGIAEHGGFDLEI-TAKTVLEGVNLGDSIAVNGTCLTVTAF 133
           +FTG+VE IG V     K+   +   G  + I     +LE V LGDSI  NG CLTVT F
Sbjct: 1   MFTGLVEHIGTVLQVTEKDTTASGGDGVSMVIGDCSKILEDVQLGDSICTNGVCLTVTEF 60

Query: 134 DHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISM 193
           D   S+FKVG++PETLR++ L EL PGS VNLERAV+   RMGGH VQGHVD    I++ 
Sbjct: 61  DMARSQFKVGISPETLRRSDLGELKPGSKVNLERAVKADVRMGGHVVQGHVDTIATIVNR 120

Query: 194 DPEGDSLWIKVKTSEALL-DYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQKAVV 253
             +G+++    K  ++    YIV KGFIA+DGTSLTV DV  E+  F+  +V+YTQ+ V+
Sbjct: 121 RGDGNAINFTFKLRDSQYGKYIVEKGFIAIDGTSLTVTDVDHEQSEFSISMVSYTQEKVI 180

Query: 254 IPLKKVGQLVNLEVDILGKYVQR 270
           +PLK  G  VN+EVD+ GK +++
Sbjct: 181 MPLKNSGDSVNIEVDLTGKLIEK 203

[Close]

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot
Match: P50854 (Riboflavin synthase OS=Actinobacillus pleuropneumoniae OX=715 GN=ribE PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.2e-45
Identity = 97/199 (48.74%), Postives = 133/199 (66.83%), Query Frame = 0


Query: 74  LFTGIVEEIGRVKNLGIAEHGGFD-LEITAKTVLEGVNLGDSIAVNGTCLTVTAFDHELS 133
           +FTGI+EE+G++    I + G F  + I A  VL+ V+LGD+IAVNG CLTVT+F    +
Sbjct: 1   MFTGIIEEVGKIAQ--IHKQGEFAVVTINATKVLQDVHLGDTIAVNGVCLTVTSFSS--N 60

Query: 134 EFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISMDPEGD 193
           +F   +  ETL++TSL EL   SPVNLERA+    R GGH V GH+DGTGEI  + P  +
Sbjct: 61  QFTADVMSETLKRTSLGELKSNSPVNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHN 120

Query: 194 SLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQKAVVIPLKKV 253
           S W ++KTS  L+ YI+ KG I +DG SLTVVD  DE  +F   ++ +T K   +  KK+
Sbjct: 121 STWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDE--SFRVSIIPHTIKETNLGSKKI 180

Query: 254 GQLVNLEVDILGKYVQRLL 272
           G +VNLE DI+GKY+++ L
Sbjct: 181 GSIVNLENDIVGKYIEQFL 193

[Close]

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot
Match: P38145 (Riboflavin synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RIB5 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-39
Identity = 92/205 (44.88%), Postives = 132/205 (64.39%), Query Frame = 0


Query: 74  LFTGIVEEIGRV---KNLGIAEHGGFDLEIT---AKTVLEGVNLGDSIAVNGTCLTVTAF 133
           +FTGIVE +G V        +E GG  + IT   A ++L   ++GDSIAVNG CLTVT F
Sbjct: 1   MFTGIVECMGTVLENNPYDDSESGGQGVSITIGNAGSILTDCHVGDSIAVNGVCLTVTEF 60

Query: 134 DHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISM 193
           +++   FKVG++PET++++++     G+ VNLERAV    R GGH+VQGHVD    I+S 
Sbjct: 61  NND--SFKVGISPETIKRSNVASWIQGTQVNLERAVSQDVRFGGHYVQGHVDTVANIVSR 120

Query: 194 DPEGDSLWIKVK-TSEALLDYIVPKGFIAVDGTSLTV--VDVFDEEKAFNFMLVAYTQKA 253
            PEG+S+    +   +    YIV KGFI +DGTSLT+  VD   +  AF   ++ +TQ  
Sbjct: 121 RPEGNSIIFGFQLRDQEYFKYIVEKGFICIDGTSLTIIKVDPLSQGGAFYISMIKHTQDN 180

Query: 254 VVIPLKKVGQLVNLEVDILGKYVQR 270
           V++PLKK+G  VN+EVD+ GK +++
Sbjct: 181 VIMPLKKIGDEVNIEVDLTGKIIEK 203

[Close]

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot
Match: P16440 (Riboflavin synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=ribE PE=1 SV=2)

HSP 1 Score: 156.0 bits (393), Expect = 6.3e-37
Identity = 86/198 (43.43%), Postives = 121/198 (61.11%), Query Frame = 0


Query: 74  LFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGTCLTVTAFDHELSE 133
           +FTGI+EE G ++++  A H    L I    +LE V+LGDSIAVNG CLTVT  D   ++
Sbjct: 1   MFTGIIEETGTIESMKKAGH-AMALTIKCSKILEDVHLGDSIAVNGICLTVT--DFTKNQ 60

Query: 134 FKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISMDPEGDS 193
           F V + PET++ TSL +L  GS VNLERA+    R GGHFV GHVDGT EI  ++ + ++
Sbjct: 61  FTVDVMPETVKATSLNDLTKGSKVNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNA 120

Query: 194 LWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQKAVVIPLKKVG 253
           ++  +K   +L   +V KG I VDG SLT+  +   E      L+ +T    +   K +G
Sbjct: 121 VYYDLKMDPSLTKTLVLKGSITVDGVSLTIFGL--TEDTVTISLIPHTISETIFSEKTIG 180

Query: 254 QLVNLEVDILGKYVQRLL 272
             VN+E D++GKY+ R L
Sbjct: 181 SKVNIECDMIGKYMYRFL 193

[Close]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL
Match: A0A6J1JEW8 (riboflavin synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111483877 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.7e-133
Identity = 245/276 (88.77%), Postives = 257/276 (93.12%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLSTA +L NIPKMVPP  + ILNVHP SSQFCP+F+ N+NRSLF LFTS  KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPRRDAILNVHPSSSQFCPRFELNTNRSLFTLFTSCRKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCK RPRSS+QCLFTGIVEEIG VK +GIAEHGGFDL+ITAKTVLEGVNLGDSI+VNGT
Sbjct: 61  LRCKPRPRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPEGDSLWIKV+TSEALL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQK VVIPLK VGQLVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQKNVVIPLKNVGQLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL
Match: L0C7R1 (LOW QUALITY PROTEIN: riboflavin synthase OS=Momordica charantia OX=3673 GN=LOC111011273 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 247/276 (89.49%), Postives = 256/276 (92.75%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           MSTFSSLSTAT L  IPKMVPPGGNTILNV P S  FC KFKFN+NRSLF LFTSS KPQ
Sbjct: 1   MSTFSSLSTATALSQIPKMVPPGGNTILNVRPGSPHFCLKFKFNTNRSLFTLFTSSCKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCKSRPR+SIQCLFTGIVEE+GRVKNLG+ E+GGFDLEI AKTVLEGV+LGDSIAVNGT
Sbjct: 61  LRCKSRPRTSIQCLFTGIVEEMGRVKNLGVTENGGFDLEIVAKTVLEGVHLGDSIAVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDHELSEF VGLAPETLRKTSLVEL PGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHELSEFTVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISM+PE DSLWIKV+TSE LL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMEPEEDSLWIKVRTSEDLLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQ +VVIPLKKVG LVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQNSVVIPLKKVGDLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL
Match: A0A6J1FU46 (riboflavin synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111448156 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.0e-132
Identity = 244/276 (88.41%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLSTA +L NIPKMVPP  + ILNV P SSQFCPKF+ N+NRSLF  FTS  KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPTRDAILNVRPSSSQFCPKFELNTNRSLFTQFTSCRKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCK RPRSS+QCLFTGIVEEIG VK +GIAEHGGFDL+ITAKTVLEGVNLGDSI+VNGT
Sbjct: 61  LRCKPRPRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPE DSLWIKV+TSEALL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEEDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQK VVIPLKKVGQLVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQKNVVIPLKKVGQLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL
Match: A0A6J1G7B0 (riboflavin synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111451517 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.5e-129
Identity = 237/278 (85.25%), Postives = 252/278 (90.65%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLS AT L +IPKM  P  N I NVHP SSQFCP  KFN  RSLF +FTSS KPQ
Sbjct: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPFLKFNPKRSLFVVFTSSQKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
             CKSR RSS+QCLFTGIVEE+GRVKNLGIAE+GGFDLEITAKTVL+GVNLGDSIAVNGT
Sbjct: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL PGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEI+SMD EGDSLW+KV+TSEA+L+YIV KGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAINS 279
           TQ AVV+PLKKVG LVNLEVD+LGKYVQRLLSN AI S
Sbjct: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGS 278

[Close]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL
Match: A0A5D3C0R4 (Riboflavin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001590 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.1e-128
Identity = 240/277 (86.64%), Postives = 255/277 (92.06%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           MSTFSSLSTAT+L NIP+M+PP G    N+H  SSQF P F FN+  SLF LFTS+HKPQ
Sbjct: 1   MSTFSSLSTATSLSNIPRMLPPMG----NLHLPSSQFFPNFNFNTKTSLFTLFTSTHKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           L  +SRPR+S+QCLFTGIVEEIG VKNLGI E+GG+DLEI AKTVLEGVNLGDSIAVNGT
Sbjct: 61  LCYRSRPRASVQCLFTGIVEEIGHVKNLGIGENGGYDLEINAKTVLEGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL+PGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELSPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPEGDSLWIKVKTSEALL+YIVPKGFIAVDGTSLTVVDVFD+EKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVKTSEALLNYIVPKGFIAVDGTSLTVVDVFDDEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAIN 278
           TQK VVIPLKKVG LVNLEVDILGKYVQRLLSN AIN
Sbjct: 241 TQKNVVIPLKKVGHLVNLEVDILGKYVQRLLSNGAIN 273

[Close]

BLAST of Lcy07g012730 vs. NCBI nr
Match: XP_038902777.1 (riboflavin synthase [Benincasa hispida])

HSP 1 Score: 501.1 bits (1289), Expect = 6.1e-138
Identity = 252/277 (90.97%), Postives = 263/277 (94.95%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           MST SSLSTAT+L NIPKMVPP GNT+LN+H  SSQFCP   FN+NRSLF LFTS+HKPQ
Sbjct: 1   MSTLSSLSTATSLSNIPKMVPPRGNTLLNLHLPSSQFCPNLNFNTNRSLFTLFTSTHKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
            RCKSRPR+S+QCLFTGIVEEIGRVK+LGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT
Sbjct: 61  FRCKSRPRTSVQCLFTGIVEEIGRVKHLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL PG+PVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTPGTPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPEGDSLWIKVKTSEALL+YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVKTSEALLNYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAIN 278
           TQK VVIPLKKVGQLVNLEVDILGKYVQRLLS  AIN
Sbjct: 241 TQKNVVIPLKKVGQLVNLEVDILGKYVQRLLSTGAIN 277

[Close]

BLAST of Lcy07g012730 vs. NCBI nr
Match: XP_022986014.1 (riboflavin synthase-like [Cucurbita maxima])

HSP 1 Score: 485.3 bits (1248), Expect = 3.4e-133
Identity = 245/276 (88.77%), Postives = 257/276 (93.12%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLSTA +L NIPKMVPP  + ILNVHP SSQFCP+F+ N+NRSLF LFTS  KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPRRDAILNVHPSSSQFCPRFELNTNRSLFTLFTSCRKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCK RPRSS+QCLFTGIVEEIG VK +GIAEHGGFDL+ITAKTVLEGVNLGDSI+VNGT
Sbjct: 61  LRCKPRPRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPEGDSLWIKV+TSEALL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQK VVIPLK VGQLVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQKNVVIPLKNVGQLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. NCBI nr
Match: XP_022140671.1 (LOW QUALITY PROTEIN: riboflavin synthase [Momordica charantia] >AFZ99647.1 riboflavin synthase [Momordica charantia])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 247/276 (89.49%), Postives = 256/276 (92.75%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           MSTFSSLSTAT L  IPKMVPPGGNTILNV P S  FC KFKFN+NRSLF LFTSS KPQ
Sbjct: 1   MSTFSSLSTATALSQIPKMVPPGGNTILNVRPGSPHFCLKFKFNTNRSLFTLFTSSCKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCKSRPR+SIQCLFTGIVEE+GRVKNLG+ E+GGFDLEI AKTVLEGV+LGDSIAVNGT
Sbjct: 61  LRCKSRPRTSIQCLFTGIVEEMGRVKNLGVTENGGFDLEIVAKTVLEGVHLGDSIAVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDHELSEF VGLAPETLRKTSLVEL PGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHELSEFTVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISM+PE DSLWIKV+TSE LL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMEPEEDSLWIKVRTSEDLLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQ +VVIPLKKVG LVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQNSVVIPLKKVGDLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. NCBI nr
Match: KAG7010374.1 (rib5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.9 bits (1239), Expect = 3.8e-132
Identity = 244/276 (88.41%), Postives = 256/276 (92.75%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLSTA +L NIPKMVPP  + ILNVHP SSQFCP+ + N+NRSLF LFTS  KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPRRDAILNVHPSSSQFCPRLELNTNRSLFTLFTSCRKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCK RPRSS+QCLFTGIVEEIG VK +GIAEHGGFDL+ITAKTVLEGVNLGDSI+VNGT
Sbjct: 61  LRCKPRPRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPE DSLWIKV+TSEALL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEEDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQK VVIPLKKVGQLVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQKNVVIPLKKVGQLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. NCBI nr
Match: XP_023513296.1 (riboflavin synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 480.3 bits (1235), Expect = 1.1e-131
Identity = 244/276 (88.41%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MSTFSSLSTATTLCNIPKMVPPGGNTILNVHPVSSQFCPKFKFNSNRSLFALFTSSHKPQ 60
           M+TFSSLSTA +L NIPKMVPP  + ILNVHP SSQFCP+F+ N NRSLF LFTS  KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPRRDAILNVHPSSSQFCPRFELNMNRSLFTLFTSCRKPQ 60

Query: 61  LRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLEITAKTVLEGVNLGDSIAVNGT 120
           LRCK R RSS+QCLFTGIVEEIG VK +GIAEHGGFDL+ITAKTVLEGVNLGDSI+VNGT
Sbjct: 61  LRCKPRQRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIISMDPE DSLWIKV+TSEALL YIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEEDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSNRAI 277
           TQK VVIPLKKVGQLVNLEVDILGKYVQRLLSN AI
Sbjct: 241 TQKNVVIPLKKVGQLVNLEVDILGKYVQRLLSNGAI 276

[Close]

BLAST of Lcy07g012730 vs. TAIR 10
Match: AT2G20690.1 (lumazine-binding family protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.1e-84
Identity = 161/234 (68.80%), Postives = 189/234 (80.77%), Query Frame = 0


Query: 40  KFKFNSNRSLFALFTSSHKPQLRCKSRPRSSIQCLFTGIVEEIGRVKNLGIAEHGGFDLE 99
           +F   S  S  +L TS  + +   + R   SI+ +FTGIVEE+G VK+LG+A+HGGFDL+
Sbjct: 31  RFDCVSKSSKLSLKTSCGRSRTHHR-RQNLSIRSVFTGIVEEMGEVKDLGMADHGGFDLK 90

Query: 100 ITAKTVLEGVNLGDSIAVNGTCLTVTAFDHELSEFKVGLAPETLRKTSLVELAPGSPVNL 159
           I A+ VLE V LGDSIAVNGTCLTVT F+ E  EF VGLAPETLRKTSL EL  GSPVNL
Sbjct: 91  IGARVVLEDVKLGDSIAVNGTCLTVTEFNAE--EFTVGLAPETLRKTSLEELKKGSPVNL 150

Query: 160 ERAVQPISRMGGHFVQGHVDGTGEIISMDPEGDSLWIKVKTSEALLDYIVPKGFIAVDGT 219
           ERA+QP+SRMGGH VQGHVDGTG I SM+ EGDSLW+KVK  + LL YIVPKGF+AVDGT
Sbjct: 151 ERALQPVSRMGGHVVQGHVDGTGVIESMEVEGDSLWVKVKADKGLLKYIVPKGFVAVDGT 210

Query: 220 SLTVVDVFDEEKAFNFMLVAYTQKAVVIPLKKVGQLVNLEVDILGKYVQRLLSN 274
           SLTVVDVFDEE  FNFM++AYTQ+ VVIP KK+GQ VNLEVDI+GKYV+RLL++
Sbjct: 211 SLTVVDVFDEESCFNFMMIAYTQQNVVIPTKKIGQKVNLEVDIMGKYVERLLTS 261

The following BLAST results are available for this feature:

BLAST of Lcy07g012730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7P0	1.3e-45	49.25	Riboflavin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28...	[more]
A5DB51	3.7e-45	49.26	Riboflavin synthase OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / D...	[more]
P50854	8.2e-45	48.74	Riboflavin synthase OS=Actinobacillus pleuropneumoniae OX=715 GN=ribE PE=3 SV=1	[more]
P38145	1.0e-39	44.88	Riboflavin synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=...	[more]
P16440	6.3e-37	43.43	Riboflavin synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=ribE PE=1 SV=...	[more]

BLAST of Lcy07g012730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JEW8	1.7e-133	88.77	riboflavin synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111483877 PE=4 SV=1	[more]
L0C7R1	2.8e-133	89.49	LOW QUALITY PROTEIN: riboflavin synthase OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FU46	7.0e-132	88.41	riboflavin synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111448156 PE=4 SV=1	[more]
A0A6J1G7B0	5.5e-129	85.25	riboflavin synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111451517 PE=4 SV=1	[more]
A0A5D3C0R4	2.1e-128	86.64	Riboflavin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G...	[more]

BLAST of Lcy07g012730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902777.1	6.1e-138	90.97	riboflavin synthase [Benincasa hispida]	[more]
XP_022986014.1	3.4e-133	88.77	riboflavin synthase-like [Cucurbita maxima]	[more]
XP_022140671.1	5.9e-133	89.49	LOW QUALITY PROTEIN: riboflavin synthase [Momordica charantia] >AFZ99647.1 ribof...	[more]
KAG7010374.1	3.8e-132	88.41	rib5, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023513296.1	1.1e-131	88.41	riboflavin synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy07g012730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20690.1	3.1e-84	68.80	lumazine-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001783	Lumazine-binding protein	TIGRFAM	TIGR00187	TIGR00187	coord: 74..275 e-value: 9.9E-61 score: 202.4
IPR001783	Lumazine-binding protein	PANTHER	PTHR21098	RIBOFLAVIN SYNTHASE ALPHA CHAIN	coord: 68..276
IPR001783	Lumazine-binding protein	CDD	cd00402	Riboflavin_synthase_like	coord: 75..263 e-value: 6.07724E-89 score: 260.398
IPR023366	ATP synthase subunit alpha, N-terminal domain-like superfamily	GENE3D	2.40.30.20		coord: 177..279 e-value: 1.7E-30 score: 106.6
IPR023366	ATP synthase subunit alpha, N-terminal domain-like superfamily	GENE3D	2.40.30.20		coord: 74..164 e-value: 1.0E-25 score: 91.4
IPR026017	Lumazine-binding domain	PFAM	PF00677	Lum_binding	coord: 175..261 e-value: 5.4E-20 score: 71.2 coord: 76..162 e-value: 1.2E-21 score: 76.5
IPR026017	Lumazine-binding domain	PROSITE	PS51177	LUMAZINE_BIND	coord: 173..271 score: 22.402388
IPR026017	Lumazine-binding domain	PROSITE	PS51177	LUMAZINE_BIND	coord: 74..172 score: 25.166328
None	No IPR available	PANTHER	PTHR21098:SF0	RIBOFLAVIN SYNTHASE	coord: 68..276
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 168..273
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 74..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g012730.1	Lcy07g012730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009231	riboflavin biosynthetic process
molecular_function	GO:0004746	riboflavin synthase activity