Lcy07g006210 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g006210
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr07: 32051050 .. 32051478 (+)
RNA-Seq ExpressionLcy07g006210
SyntenyLcy07g006210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGCTTCTGGTTTTCGAAATGTCATGTGGAAATGGAGGATGTGCTGGTTGGAAGCCCTAGCAGCGCTTGAAGGTGTGAGGACGATTCCAAAAAACTCCCTCAAGGTATTCATCGAGCTTGACTCTAGTCAGGTGGTGAATCTATTGTTAGGGAGAGAGGAAGATGTAATTGAAATCTCACAGTTCATTGAAGAAGCCCAAGCATTGATCTCGGCTGCAAATGTGGTCTCCATCTTTCACATATCTAGAAGAAGTAACTATATGGCCCATCATCTTGCTAGATCAGGATGGGCTTTAAACTCTTCAGAGTGTTGGTTTAATATTTTCCCTCTTTGGGTTTCAAAATTGAATGTTTTGGATATTAGGGTTGATAGTTGCATTAGTGGGGGTGCCTGTCTCACTAATGGAAGCCTTTGCTATTTTTAA

mRNA sequence

ATGGTGGCTTCTGGTTTTCGAAATGTCATGTGGAAATGGAGGATGTGCTGGTTGGAAGCCCTAGCAGCGCTTGAAGGTGTGAGGACGATTCCAAAAAACTCCCTCAAGGTATTCATCGAGCTTGACTCTAGTCAGGTGGTGAATCTATTGTTAGGGAGAGAGGAAGATGTAATTGAAATCTCACAGTTCATTGAAGAAGCCCAAGCATTGATCTCGGCTGCAAATGTGGTCTCCATCTTTCACATATCTAGAAGAAGTAACTATATGGCCCATCATCTTGCTAGATCAGGATGGGCTTTAAACTCTTCAGAGTGTTGGTTTAATATTTTCCCTCTTTGGGTTTCAAAATTGAATGTTTTGGATATTAGGGTTGATAGTTGCATTAGTGGGGGTGCCTGTCTCACTAATGGAAGCCTTTGCTATTTTTAA

Coding sequence (CDS)

ATGGTGGCTTCTGGTTTTCGAAATGTCATGTGGAAATGGAGGATGTGCTGGTTGGAAGCCCTAGCAGCGCTTGAAGGTGTGAGGACGATTCCAAAAAACTCCCTCAAGGTATTCATCGAGCTTGACTCTAGTCAGGTGGTGAATCTATTGTTAGGGAGAGAGGAAGATGTAATTGAAATCTCACAGTTCATTGAAGAAGCCCAAGCATTGATCTCGGCTGCAAATGTGGTCTCCATCTTTCACATATCTAGAAGAAGTAACTATATGGCCCATCATCTTGCTAGATCAGGATGGGCTTTAAACTCTTCAGAGTGTTGGTTTAATATTTTCCCTCTTTGGGTTTCAAAATTGAATGTTTTGGATATTAGGGTTGATAGTTGCATTAGTGGGGGTGCCTGTCTCACTAATGGAAGCCTTTGCTATTTTTAA

Protein sequence

MVASGFRNVMWKWRMCWLEALAALEGVRTIPKNSLKVFIELDSSQVVNLLLGREEDVIEISQFIEEAQALISAANVVSIFHISRRSNYMAHHLARSGWALNSSECWFNIFPLWVSKLNVLDIRVDSCISGGACLTNGSLCYF
Homology
BLAST of Lcy07g006210 vs. ExPASy TrEMBL
Match: F4NCI7 (Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris OX=3555 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.3e-04
Identity = 35/119 (29.41%), Postives = 58/119 (48.74%), Query Frame = 0

Query: 5    GFRNVMWKWRMCWLEALAALEGVRTIPKNSL-KVFIELDSSQVVNLLLGREEDVIEISQF 64
            G + V  +W     EA+AAL  V    +    ++ +E D+  V+N +  + E V  + + 
Sbjct: 1243 GVKRVAARWTAVMAEAMAALFAVEVAHRLGFGRIVLEGDAMMVINAVKHKCEGVAPMFRI 1302

Query: 65   IEEAQALISAANVVSIFHISRRSNYMAHHLARSGWALNSSECWFNIFPLWVSKLNVLDI 123
              +  +L +  +V S+ H+ R  N +AH LAR     NS   W + FP  +S L  LD+
Sbjct: 1303 FNDISSLGACLDVFSVSHVRRAGNTVAHLLARWCCDCNSEIVWLDSFPQSISTLAELDL 1361

BLAST of Lcy07g006210 vs. NCBI nr
Match: KAG2711776.1 (hypothetical protein I3760_04G092800 [Carya illinoinensis])

HSP 1 Score: 57.8 bits (138), Expect = 9.0e-05
Identity = 33/112 (29.46%), Postives = 64/112 (57.14%), Query Frame = 0

Query: 17   WLEALAALEGVRTIPKNSL-KVFIELDSSQVVNLLLGREEDVIEISQFIEEAQALISAAN 76
            ++EA+A L G++   +  + K+ +E D   +VN L G  E + + +  +++ + L++A  
Sbjct: 1681 FIEAVALLRGLQLCVQWGIPKLMLETDCLILVNALNGNSECLTDFAFILQDIRRLMAALQ 1740

Query: 77   VVSIFHISRRSNYMAHHLARSGWALNSSECWFNIFPLWVSKLNVLDIRVDSC 128
             V + H++R  N +AH LAR  W ++    W++  P +VS+   LD ++D C
Sbjct: 1741 EVKVVHVNRLGNQVAHLLARHAWLIDDICMWWDFCPSFVSQAIWLD-QLDIC 1791

BLAST of Lcy07g006210 vs. NCBI nr
Match: XP_024033599.1 (uncharacterized protein LOC112095720 [Citrus clementina])

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-04
Identity = 36/95 (37.89%), Postives = 52/95 (54.74%), Query Frame = 0

Query: 2   VASGFRNVMWKWRMCWLEALAALEGVRTIPK-NSLKVFIELDSSQVVNLLLGREEDVIEI 61
           VA+  +    K  +  +EA A L G++   K N     IE DS +VV L+L R+  ++EI
Sbjct: 151 VAAAAQRTTLKGNVADMEAEAVLLGIQVARKANCAHFVIESDSKEVVELVLKRKRSLVEI 210

Query: 62  SQFIEEAQALISAANVVSIFHISRRSNYMAHHLAR 96
           S  IEE Q  +   N  SI ++ RR N  AH+LA+
Sbjct: 211 SWNIEEIQECLKGQNTTSILYVPRRCNVRAHNLAK 245

BLAST of Lcy07g006210 vs. NCBI nr
Match: XP_015387358.1 (uncharacterized protein LOC107177672 [Citrus sinensis])

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-04
Identity = 32/118 (27.12%), Postives = 64/118 (54.24%), Query Frame = 0

Query: 1   MVASGFRNVMWKWRMCWLEALAALEGVRTIPK-NSLKVFIELDSSQVVNLLLGREEDVIE 60
           ++A+  + V ++  +  +EA A L G++   +   L + IE DS++VV L+  R+  + E
Sbjct: 261 IIAAAVKKVCFQGTVASMEAEAVLFGIQVAQQVEYLPMIIESDSTEVVELVWSRKSSLTE 320

Query: 61  ISQFIEEAQALISAANVVSIFHISRRSNYMAHHLARSGWALNSSECWFNIFPLWVSKL 118
           +S  +EE +  +  +N  S+ ++ R+ N +AH +A+      +S  W   FP+ +  L
Sbjct: 321 VSWTVEEIKQQLQISNASSLQYVPRKCNVIAHAIAKVALDFENSVIWLEDFPVQIMML 378

BLAST of Lcy07g006210 vs. NCBI nr
Match: XP_024042448.1 (uncharacterized protein LOC112099303 [Citrus clementina])

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-04
Identity = 36/95 (37.89%), Postives = 53/95 (55.79%), Query Frame = 0

Query: 2   VASGFRNVMWKWRMCWLEALAALEGVRTIPKNSLKVF-IELDSSQVVNLLLGREEDVIEI 61
           VA+  +    K  +  +EA A L G++   K +   F IE DS +VV L+L R+  ++EI
Sbjct: 131 VAAAAQRTTLKGNVADMEAEAVLLGIQVARKANCPHFVIESDSKEVVELVLKRKRSLVEI 190

Query: 62  SQFIEEAQALISAANVVSIFHISRRSNYMAHHLAR 96
           S  IEE Q  +   N  SI ++ RR N  AH+LA+
Sbjct: 191 SWNIEEIQECLKGQNTASILYVPRRCNVRAHNLAK 225

BLAST of Lcy07g006210 vs. NCBI nr
Match: XP_015388376.1 (uncharacterized protein LOC102612209 [Citrus sinensis])

HSP 1 Score: 55.1 bits (131), Expect = 5.8e-04
Identity = 34/118 (28.81%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 1   MVASGFRNVMWKWRMCWLEALAALEGVRTIPK-NSLKVFIELDSSQVVNLLLGREEDVIE 60
           ++A+  + V +K  +  +EA A L G++   +   L + IE DS++VV L+L R+  + E
Sbjct: 213 IIAAAVKRVSYKGTVACMEAEAILFGIQVAQQAECLPMIIESDSTEVVELVLNRKGSMTE 272

Query: 61  ISQFIEEAQALISAANVVSIFHISRRSNYMAHHLARSGWALNSSECWFNIFPLWVSKL 118
           IS  +EE +  +   N  SI    R+ N +AH + +  +   +   W   FP+ +  L
Sbjct: 273 ISWTVEEIKQRLMNHNTSSIQFAPRKCNAIAHSIVKVAFDFENPVIWLEEFPVQIMLL 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
F4NCI76.3e-0429.41Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris... [more]
Match NameE-valueIdentityDescription
KAG2711776.19.0e-0529.46hypothetical protein I3760_04G092800 [Carya illinoinensis][more]
XP_024033599.11.2e-0437.89uncharacterized protein LOC112095720 [Citrus clementina][more]
XP_015387358.11.2e-0427.12uncharacterized protein LOC107177672 [Citrus sinensis][more]
XP_024042448.11.5e-0437.89uncharacterized protein LOC112099303 [Citrus clementina][more]
XP_015388376.15.8e-0428.81uncharacterized protein LOC102612209 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 16..102
e-value: 2.1E-7
score: 33.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 19..96
e-value: 4.1E-10
score: 39.5
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 16..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g006210.1Lcy07g006210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity