Lcy07g004380 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g004380
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Retrotransposon protein
Location	Chr07: 21854110 .. 21854531 (-)
RNA-Seq Expression	Lcy07g004380
Synteny	Lcy07g004380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCTTATCGACCCAGATACCAAACGAGGAAGGGCGAGATCACAACGAATGTGCTTGTTGTTTGTTCGCAGAATGGCGAGTTCATACTCGTTTTCCCGGGGTGGGAATGATCTGCATCCGACTCAAGAGTTCTTCGCGATGTAGTATCACGTCCGATAGGGCTGAAGGTTCTCAAGGGCTACTACTACCTATGCGACGTCGGTTATTCGAAAGTTGAGGGATTTCTAGCACCCTACTGCAGAACACGATACAACCTCACGAAGTGGCGAGGTGGAAATCCACAAACGATCCCAAAAGAGCTTTTCAACATGAGACATTCATCCGCTCGAAATGTAATAGAACGAGCTTTCAGTGTGTTGAAGGGTCGATGGGCCATTATGCAGGGGAAGTCGTACTATCCCGTCGACATCCAAAAATAA

mRNA sequence

ATGCAGCTTATCGACCCAGATACCAAACGAGGAAGGGCGAGATCACAACGAATGTGCTTGTTGTTTGTTCGCAGAATGGCGAGTTCATACTCGTTTTCCCGGGTATCACGTCCGATAGGGCTGAAGGTTCTCAAGGGCTACTACTACCTATGCGACGTCGGTTATTCGAAAGTTGAGGGATTTCTAGCACCCTACTGCAGAACACGATACAACCTCACGAAGTGGCGAGGTGGAAATCCACAAACGATCCCAAAAGAGCTTTTCAACATGAGACATTCATCCGCTCGAAATGTAATAGAACGAGCTTTCAGTGTGTTGAAGGGTCGATGGGCCATTATGCAGGGGAAGTCGTACTATCCCGTCGACATCCAAAAATAA

Coding sequence (CDS)

ATGCAGCTTATCGACCCAGATACCAAACGAGGAAGGGCGAGATCACAACGAATGTGCTTGTTGTTTGTTCGCAGAATGGCGAGTTCATACTCGTTTTCCCGGGTATCACGTCCGATAGGGCTGAAGGTTCTCAAGGGCTACTACTACCTATGCGACGTCGGTTATTCGAAAGTTGAGGGATTTCTAGCACCCTACTGCAGAACACGATACAACCTCACGAAGTGGCGAGGTGGAAATCCACAAACGATCCCAAAAGAGCTTTTCAACATGAGACATTCATCCGCTCGAAATGTAATAGAACGAGCTTTCAGTGTGTTGAAGGGTCGATGGGCCATTATGCAGGGGAAGTCGTACTATCCCGTCGACATCCAAAAATAA

Protein sequence

MQLIDPDTKRGRARSQRMCLLFVRRMASSYSFSRVSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSSARNVIERAFSVLKGRWAIMQGKSYYPVDIQK

Homology

[Close]

BLAST of Lcy07g004380 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 7.0e-04
Identity = 24/73 (32.88%), Postives = 33/73 (45.21%), Query Frame = 0


Query: 48  YYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSSARNVIERAFSVLK 107
           Y + D G+  +   L PY            G P ++P+  FN RHS A    + A S LK
Sbjct: 275 YIVGDSGFPLLPWLLTPY-----------QGKPTSLPQTEFNKRHSEATKAAQMALSKLK 334

Query: 108 GRWAIMQGKSYYP 121
            RW I+ G  + P
Sbjct: 335 DRWRIINGVMWMP 336

[Close]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL
Match: A0A5A7U3R2 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G001010 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.5e-33
Identity = 70/90 (77.78%), Postives = 75/90 (83.33%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VSRPIGLKV KGYYYLCD  Y  VEGFLAPY   RY+L +WRGGNP   PKELFNMRHSS
Sbjct: 170 VSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHLIEWRGGNPPKCPKELFNMRHSS 229

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 230 ARNVIERAFGSLKSRWAILRGRSYYPVDIQ 259

[Close]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL
Match: A0A5D3C7X6 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002160 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.5e-33
Identity = 70/90 (77.78%), Postives = 75/90 (83.33%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VSRPIGLKV KGYYYLCD  Y  VEGFLAPY   RY+L +WRGGNP   PKELFNMRHSS
Sbjct: 148 VSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHLIEWRGGNPPKCPKELFNMRHSS 207

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 208 ARNVIERAFGSLKSRWAILRGRSYYPVDIQ 237

[Close]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL
Match: A0A5D3D2G7 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G001300 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 7.4e-33
Identity = 70/92 (76.09%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 33  SRVSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRH 92
           SRVSRP  LKVLKGYYYLCDVGY  VEGFLAPY   RY LT+WRGGNP   PKELFNMR+
Sbjct: 71  SRVSRPTCLKVLKGYYYLCDVGYLNVEGFLAPYRGQRYRLTEWRGGNPPKCPKELFNMRY 130

Query: 93  SSARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           SS RNVIER F  LKG+WAI++G+SY PVDIQ
Sbjct: 131 SSIRNVIERTFRSLKGQWAILRGRSYCPVDIQ 162

[Close]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL
Match: A0A5A7TKM2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G003450 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 7.4e-33
Identity = 70/92 (76.09%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 33  SRVSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRH 92
           SRVSRP  LKVLKGYYYLCDVGY  VEGFLAPY   RY LT+WRGGNP   PKELFNMR+
Sbjct: 75  SRVSRPTCLKVLKGYYYLCDVGYLNVEGFLAPYRGQRYRLTEWRGGNPPKCPKELFNMRY 134

Query: 93  SSARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           SS RNVIER F  LKG+WAI++G+SY PVDIQ
Sbjct: 135 SSIRNVIERTFRSLKGQWAILRGRSYCPVDIQ 166

[Close]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL
Match: A0A5D3C3H1 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002470 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.1e-31
Identity = 66/90 (73.33%), Postives = 72/90 (80.00%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VS+P GLK  KGYYYLCD GY   EGFLAPY    Y+LT+WRGGNP   PKELFNMRHS 
Sbjct: 104 VSQPSGLKAPKGYYYLCDTGYLNAEGFLAPYRGQHYHLTEWRGGNPPKWPKELFNMRHSY 163

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 164 ARNVIERAFGSLKDRWAILRGRSYYPVDIQ 193

[Close]

BLAST of Lcy07g004380 vs. NCBI nr
Match: KAA0048191.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 3.1e-33
Identity = 70/90 (77.78%), Postives = 75/90 (83.33%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VSRPIGLKV KGYYYLCD  Y  VEGFLAPY   RY+L +WRGGNP   PKELFNMRHSS
Sbjct: 170 VSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHLIEWRGGNPPKCPKELFNMRHSS 229

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 230 ARNVIERAFGSLKSRWAILRGRSYYPVDIQ 259

[Close]

BLAST of Lcy07g004380 vs. NCBI nr
Match: TYK08067.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 3.1e-33
Identity = 70/90 (77.78%), Postives = 75/90 (83.33%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VSRPIGLKV KGYYYLCD  Y  VEGFLAPY   RY+L +WRGGNP   PKELFNMRHSS
Sbjct: 148 VSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHLIEWRGGNPPKCPKELFNMRHSS 207

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 208 ARNVIERAFGSLKSRWAILRGRSYYPVDIQ 237

[Close]

BLAST of Lcy07g004380 vs. NCBI nr
Match: TYK17860.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 149.8 bits (377), Expect = 1.5e-32
Identity = 70/92 (76.09%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 33  SRVSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRH 92
           SRVSRP  LKVLKGYYYLCDVGY  VEGFLAPY   RY LT+WRGGNP   PKELFNMR+
Sbjct: 71  SRVSRPTCLKVLKGYYYLCDVGYLNVEGFLAPYRGQRYRLTEWRGGNPPKCPKELFNMRY 130

Query: 93  SSARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           SS RNVIER F  LKG+WAI++G+SY PVDIQ
Sbjct: 131 SSIRNVIERTFRSLKGQWAILRGRSYCPVDIQ 162

[Close]

BLAST of Lcy07g004380 vs. NCBI nr
Match: KAA0041929.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 149.8 bits (377), Expect = 1.5e-32
Identity = 70/92 (76.09%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 33  SRVSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRH 92
           SRVSRP  LKVLKGYYYLCDVGY  VEGFLAPY   RY LT+WRGGNP   PKELFNMR+
Sbjct: 75  SRVSRPTCLKVLKGYYYLCDVGYLNVEGFLAPYRGQRYRLTEWRGGNPPKCPKELFNMRY 134

Query: 93  SSARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           SS RNVIER F  LKG+WAI++G+SY PVDIQ
Sbjct: 135 SSIRNVIERTFRSLKGQWAILRGRSYCPVDIQ 166

[Close]

BLAST of Lcy07g004380 vs. NCBI nr
Match: TYK05758.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 144.4 bits (363), Expect = 6.4e-31
Identity = 66/90 (73.33%), Postives = 72/90 (80.00%), Query Frame = 0


Query: 35  VSRPIGLKVLKGYYYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSS 94
           VS+P GLK  KGYYYLCD GY   EGFLAPY    Y+LT+WRGGNP   PKELFNMRHS 
Sbjct: 104 VSQPSGLKAPKGYYYLCDTGYLNAEGFLAPYRGQHYHLTEWRGGNPPKWPKELFNMRHSY 163

Query: 95  ARNVIERAFSVLKGRWAIMQGKSYYPVDIQ 125
           ARNVIERAF  LK RWAI++G+SYYPVDIQ
Sbjct: 164 ARNVIERAFGSLKDRWAILRGRSYYPVDIQ 193

[Close]

BLAST of Lcy07g004380 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 65.5 bits (158), Expect = 3.5e-11
Identity = 37/80 (46.25%), Postives = 50/80 (62.50%), Query Frame = 0


Query: 48  YYLCDVGYSKVEGFLAPYCRTRYNLTKWRG--GNPQTIPKELFNMRHSSARNVIERAFSV 107
           +YL D G++    FLAP+   RY+L ++ G   +P+T P ELFN+RH S RNVIER F +
Sbjct: 49  FYLVDCGFANRLNFLAPFRGVRYHLQEFAGQRRDPET-PHELFNLRHVSLRNVIERIFGI 108

Query: 108 LKGRWAIMQGKSYYPVDIQK 126
            K R+AI   KS  P   +K
Sbjct: 109 FKSRFAIF--KSAPPFSYKK 125

[Close]

BLAST of Lcy07g004380 vs. TAIR 10
Match: AT4G10890.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439 (InterPro:IPR018838); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 58.2 bits (139), Expect = 5.7e-09
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 0


Query: 48  YYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSSARNVIERAFSVLK 107
           YYL +  Y    G+L P+ R  Y+L ++  G P    +ELFN +H   R+VI+R F V K
Sbjct: 95  YYLVNSVYPTTTGYLGPHRRILYHLGQFGRGGPPVTVQELFNRKHLDLRSVIDRTFGVWK 154

Query: 108 GRWAIM 114
            +W I+
Sbjct: 155 AKWRIL 160

[Close]

BLAST of Lcy07g004380 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 6.9e-07
Identity = 27/67 (40.30%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 48  YYLCDVGYSKVEGFLAPYCRT-----RYNLTKWRGGNPQTIPKELFNMRHSSARNVIERA 107
           YYL D GY   +G LAPY  +     RY+++++  G       ELFN  H+S R+VIER 
Sbjct: 253 YYLVDSGYPNKQGLLAPYRSSRNRVVRYHMSQFYYGPRPRNKHELFNQCHTSLRSVIERT 312

Query: 108 FSVLKGR 110
           F + K +
Sbjct: 313 FRIWKNK 319

[Close]

BLAST of Lcy07g004380 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 45.1 bits (105), Expect = 5.0e-05
Identity = 24/73 (32.88%), Postives = 33/73 (45.21%), Query Frame = 0


Query: 48  YYLCDVGYSKVEGFLAPYCRTRYNLTKWRGGNPQTIPKELFNMRHSSARNVIERAFSVLK 107
           Y + D G+  +   L PY            G P ++P+  FN RHS A    + A S LK
Sbjct: 275 YIVGDSGFPLLPWLLTPY-----------QGKPTSLPQTEFNKRHSEATKAAQMALSKLK 334

Query: 108 GRWAIMQGKSYYP 121
            RW I+ G  + P
Sbjct: 335 DRWRIINGVMWMP 336

The following BLAST results are available for this feature:

BLAST of Lcy07g004380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U3	7.0e-04	32.88	Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1	[more]

BLAST of Lcy07g004380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U3R2	1.5e-33	77.78	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3C7X6	1.5e-33	77.78	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3D2G7	7.4e-33	76.09	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7TKM2	7.4e-33	76.09	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A5D3C3H1	3.1e-31	73.33	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Lcy07g004380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048191.1	3.1e-33	77.78	retrotransposon protein [Cucumis melo var. makuwa]	[more]
TYK08067.1	3.1e-33	77.78	retrotransposon protein [Cucumis melo var. makuwa]	[more]
TYK17860.1	1.5e-32	76.09	putative nuclease HARBI1 [Cucumis melo var. makuwa]	[more]
KAA0041929.1	1.5e-32	76.09	putative nuclease HARBI1 [Cucumis melo var. makuwa]	[more]
TYK05758.1	6.4e-31	73.33	putative nuclease HARBI1 [Cucumis melo var. makuwa]	[more]

BLAST of Lcy07g004380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35695.1	3.5e-11	46.25	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT4G10890.1	5.7e-09	39.39	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439...	[more]
AT1G43722.1	6.9e-07	40.30	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G55350.1	5.0e-05	32.88	PIF / Ping-Pong family of plant transposases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027806	Harbinger transposase-derived nuclease domain	PFAM	PF13359	DDE_Tnp_4	coord: 41..116 e-value: 1.1E-8 score: 34.9
None	No IPR available	PANTHER	PTHR22930	UNCHARACTERIZED	coord: 35..116
None	No IPR available	PANTHER	PTHR22930:SF211	NUCLEASE HARBI1-RELATED	coord: 35..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g004380.1	Lcy07g004380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding