Lcy07g004240 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g004240
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand family protein, putative
Location	Chr07: 20268208 .. 20269301 (-)
RNA-Seq Expression	Lcy07g004240
Synteny	Lcy07g004240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAATAAAAAAAAATAAAAAATAAAATCTTTTTCTATTGCCTATAAATAAGGTTCGTGGTCTCCCCCAAAAACTCAACGTCTCCCATTGATCTCAAAAACCCTTAAAAAACTTCACCACTTACGAATTGTTTCTCTACTCAAAACCCAGAAAGAAGATGACAAACAAAGGCAGAAGCGTACTGAGCTTATTCACAAGCTGTTCACGAAAGCGACAAATTAAACAAATTCCCACATCCACCCAACTCAAGCCGAGGTCCAACTCCTCAACCTCCTCAAAGCACTTCCGCCCAAACTTGGAGGAGGTCAAATGGGTATGAACAAATAAATATTCATACATTTAACATGCTATCGATATCCAATCCTGTGTTCGAACTCCTGTAATATCATTTTTTATATCTTTTAATAGGCGTTCGACAAGTTCGACTCCAACAAGGATGGTAAGATCTCCTTTGAGGAGTACAAGGCTGCTCACAGAGCCATGGCTGGAAGCAAAGAGATCTCCGACGCCGAGGCTGCGAAGTCGTTCGAGTTTGTCGATGTGGATGGAGATGGATTCGTGGACTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCGGTGGGGAAGTGAGGGTAGGGGACATCGAGAGCGCGTTCAAAGTGTACGATTCGAATGGAGATGGGAAGATAAGTGCCGAGGAAGTGATGGAGATCATGAAGATATTGGGCGAGCGGACCAACCTTAAGGCGTGCAGGCAAATGGTGAAGGGTGTGGATACAGATGGAGATGGATTTATTGATATGAAGGAGTTTTCGAAGCTCATGGGTTCGTATTCATTTAAGTTTCAGAATTTATGAGGTTAATAGTTTTTGTATCGTTGAATGTATGAAAGAAATTTTAAGCTATACGTACTTTGACGTATATCGAATAGTTAGGAGGATAATTATGTTGATGTTACGGCCATAAGTAGGTCTTGTTTTCTTTCTTTATTTTTCTTCTTTGTTGTGTGATGTTAGCGTTGATTATGTGCCACTACTAGTAGTTGTATGGAAAGAGTGGATGTGATGATGTGAGGGAATATGAATTTATAACATTGACTTCTCTCTCTCTCTTT

mRNA sequence

GAGAATAAAAAAAAATAAAAAATAAAATCTTTTTCTATTGCCTATAAATAAGGTTCGTGGTCTCCCCCAAAAACTCAACGTCTCCCATTGATCTCAAAAACCCTTAAAAAACTTCACCACTTACGAATTGTTTCTCTACTCAAAACCCAGAAAGAAGATGACAAACAAAGGCAGAAGCGTACTGAGCTTATTCACAAGCTGTTCACGAAAGCGACAAATTAAACAAATTCCCACATCCACCCAACTCAAGCCGAGGTCCAACTCCTCAACCTCCTCAAAGCACTTCCGCCCAAACTTGGAGGAGGTCAAATGGGCGTTCGACAAGTTCGACTCCAACAAGGATGGTAAGATCTCCTTTGAGGAGTACAAGGCTGCTCACAGAGCCATGGCTGGAAGCAAAGAGATCTCCGACGCCGAGGCTGCGAAGTCGTTCGAGTTTGTCGATGTGGATGGAGATGGATTCGTGGACTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCGGTGGGGAAGTGAGGGTAGGGGACATCGAGAGCGCGTTCAAAGTGTACGATTCGAATGGAGATGGGAAGATAAGTGCCGAGGAAGTGATGGAGATCATGAAGATATTGGGCGAGCGGACCAACCTTAAGGCGTGCAGGCAAATGGTGAAGGGTGTGGATACAGATGGAGATGGATTTATTGATATGAAGGAGTTTTCGAAGCTCATGGGTTCGTATTCATTTAAGTTTCAGAATTTATGAGGTTAATAGTTTTTGTATCGTTGAATGTATGAAAGAAATTTTAAGCTATACGTACTTTGACGTATATCGAATAGTTAGGAGGATAATTATGTTGATGTTACGGCCATAAGTAGGTCTTGTTTTCTTTCTTTATTTTTCTTCTTTGTTGTGTGATGTTAGCGTTGATTATGTGCCACTACTAGTAGTTGTATGGAAAGAGTGGATGTGATGATGTGAGGGAATATGAATTTATAACATTGACTTCTCTCTCTCTCTTT

Coding sequence (CDS)

ATGACAAACAAAGGCAGAAGCGTACTGAGCTTATTCACAAGCTGTTCACGAAAGCGACAAATTAAACAAATTCCCACATCCACCCAACTCAAGCCGAGGTCCAACTCCTCAACCTCCTCAAAGCACTTCCGCCCAAACTTGGAGGAGGTCAAATGGGCGTTCGACAAGTTCGACTCCAACAAGGATGGTAAGATCTCCTTTGAGGAGTACAAGGCTGCTCACAGAGCCATGGCTGGAAGCAAAGAGATCTCCGACGCCGAGGCTGCGAAGTCGTTCGAGTTTGTCGATGTGGATGGAGATGGATTCGTGGACTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCGGTGGGGAAGTGAGGGTAGGGGACATCGAGAGCGCGTTCAAAGTGTACGATTCGAATGGAGATGGGAAGATAAGTGCCGAGGAAGTGATGGAGATCATGAAGATATTGGGCGAGCGGACCAACCTTAAGGCGTGCAGGCAAATGGTGAAGGGTGTGGATACAGATGGAGATGGATTTATTGATATGAAGGAGTTTTCGAAGCTCATGGGTTCGTATTCATTTAAGTTTCAGAATTTATGA

Protein sequence

MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPRSNSSTSSKHFRPNLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSKLMGSYSFKFQNL

Homology

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BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.2e-32
Identity = 80/184 (43.48%), Postives = 117/184 (63.59%), Query Frame = 0


Query: 7   SVLSLFTSCSRKRQIKQIPTSTQLKPRSNSSTSSK----HFRPNLEEVKWAFDKFDSNKD 66
           S L L    S+ + +++ P+    + R +S  SS       +P++ E++  F +FD +KD
Sbjct: 5   SFLELQYKLSKNKMLRK-PSRMFSRDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKD 64

Query: 67  GKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVG 126
           GKIS  EYK   RA+   + I D    K F+ VD+DGDGF+D +EF++ Y  SGG +R  
Sbjct: 65  GKISQTEYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSS 124

Query: 127 DIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSK 186
           DI ++F  +D NGDGKISAEEVM ++  LGER +L+ C +MV+ VD DGDG ++M+EF K
Sbjct: 125 DIRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIK 184

[Close]

BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 5.5e-24
Identity = 60/146 (41.10%), Postives = 95/146 (65.07%), Query Frame = 0


Query: 46  NLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDL 105
           +++++K  F +FD N DGKIS +E K   RA+  S   S  E     +  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 106 KEFVELYTM---SGGEVR--VGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKAC 165
            EFV L+ +    GG  R  V D++ AF++YD +G+G+ISA+E+  +MK LGE+ +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 166 RQMVKGVDTDGDGFIDMKEFSKLMGS 187
           ++M+  VD DGDG ++  EF K+M +
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMMSN 157

[Close]

BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.6e-23
Identity = 59/152 (38.82%), Postives = 94/152 (61.84%), Query Frame = 0


Query: 36  SSTSSKHFRPNLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFV 95
           S   S++   ++E++K  F +FD N DGKIS +E K    A+  S   S  E     +  
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEF 61

Query: 96  DVDGDGFVDLKEFVELYTM---SGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILG 155
           D+DG+GF+DL EFV L+ +   S     + D++ AF +YD + +G+ISA E+  +MK LG
Sbjct: 62  DLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG 121

Query: 156 ERTNLKACRQMVKGVDTDGDGFIDMKEFSKLM 185
           E+ +++ C++M+  VD+DGDG +D +EF K+M
Sbjct: 122 EKCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

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BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot
Match: Q9LF55 (Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.0e-21
Identity = 60/143 (41.96%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 54  FDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYT 113
           F++ D NKDGKIS++E+  A RA   S  I+  E    F  +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIRAF--SPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 114 MSGGEVRVGD----IESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDT 173
           M GGE    D    ++ AF +YD +GDGKISA E+  ++K LGE+  +  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 174 DGDGFIDMKEFSKLMGSYSFKFQ 193
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

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BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.6e-21
Identity = 58/143 (40.56%), Postives = 91/143 (63.64%), Query Frame = 0


Query: 44  RPNLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFV 103
           +  + E++  F KFD N DGKIS +E  A   ++    E+ + E  K+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 104 DLKEFVELYT--MSGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACR 163
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+ E+++ LG+  ++  CR
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 164 QMVKGVDTDGDGFIDMKEFSKLM 185
           +M+ GVD DGDG ID +EF  +M
Sbjct: 152 KMIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL
Match: A0A6J1G5E9 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.7e-81
Identity = 162/194 (83.51%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLSLFTSCS KRQ+KQIP+STQ K R    S+SS SSKH R NLEE+KW FDK
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAH AM+GSKEISDAEAAKSF+ VD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GDI+SAFKVYDSNGDGKISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           + EFS LMGSYSFK
Sbjct: 181 IDEFSSLMGSYSFK 194

[Close]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL
Match: A0A6J1L0C4 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.3e-80
Identity = 158/194 (81.44%), Postives = 176/194 (90.72%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLSLFTSCS KRQ+KQIP+STQ K R    S+SS SSKH + NLEE+KWAF+K
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRFKSISDSSASSKHVQVNLEEIKWAFEK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAHRAM+GS+EISD EAAKSF+FVD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHRAMSGSQEISDVEAAKSFKFVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GD++SAFKVYDSNGDG+ISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDVQSAFKVYDSNGDGRISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           + EFS LMG+YSFK
Sbjct: 181 IDEFSSLMGTYSFK 194

[Close]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL
Match: A0A6J1D869 (calmodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111017929 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.3e-78
Identity = 153/184 (83.15%), Postives = 165/184 (89.67%), Query Frame = 0


Query: 8   VLSLFTSCSRKRQIKQIPTSTQLKPRSNSSTSSKHFRPNLEEVKWAFDKFDSNKDGKISF 67
           +L LFTSCSRKRQ KQ P +TQLKPRSNSST SKH RPNLEE+KW FDKFD ++DGKISF
Sbjct: 1   MLRLFTSCSRKRQPKQTPAATQLKPRSNSSTYSKHPRPNLEEIKWVFDKFDKDRDGKISF 60

Query: 68  EEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVGDIESA 127
           EEYKAAHRAMAG+KEIS+AEAAKSF FVD DGDG+VDLKEFVELY  +GGEVRVGDIESA
Sbjct: 61  EEYKAAHRAMAGNKEISEAEAAKSFGFVDGDGDGYVDLKEFVELYATNGGEVRVGDIESA 120

Query: 128 FKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSKLMGSY 187
           FKVYDSNGDGKISAEEVM IMK+LGE TNL  CR MVKGVD DGDGFID+KEF+KLMGSY
Sbjct: 121 FKVYDSNGDGKISAEEVMGIMKVLGESTNLTVCRAMVKGVDGDGDGFIDIKEFAKLMGSY 180

Query: 188 SFKF 192
           S KF
Sbjct: 181 SLKF 184

[Close]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL
Match: A0A0A0LMP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.9e-75
Identity = 152/188 (80.85%), Postives = 164/188 (87.23%), Query Frame = 0


Query: 7   SVLSLFTSCSRKRQIKQIPTSTQLKPRSNSST-SSKHFRPNLEEVKWAFDKFDSNKDGKI 66
           S+L LF SCS KR  K    S  +K RSNSS+ SSKHFRPNLEE+KWAF+KFDSNKDGKI
Sbjct: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDGKI 62

Query: 67  SFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVGDIE 126
           SFEEYK AHR +AGSKEI+DAEA KSF+ VDVDGDGFVDLKEFVELYTMS GEV+VGDIE
Sbjct: 63  SFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIE 122

Query: 127 SAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSKLMG 186
           SAFKVYDSNGDGKISAEEVM IMKILGE T LKAC+QMVKGVD DGDGFID++EFSKLMG
Sbjct: 123 SAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLMG 182

Query: 187 SYSFKFQN 194
            YS KFQN
Sbjct: 183 KYSSKFQN 190

[Close]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL
Match: A0A5D3BJJ3 (Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00260 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.5e-74
Identity = 150/187 (80.21%), Postives = 162/187 (86.63%), Query Frame = 0


Query: 7   SVLSLFTSCSRKRQIKQIPTSTQLKPRSN-SSTSSKHFRPNLEEVKWAFDKFDSNKDGKI 66
           S+L LF SCS KR  K    S Q+K RSN SSTSSKHFRPNLEE+KWAF+KFDSNKDGKI
Sbjct: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKI 62

Query: 67  SFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVGDIE 126
           SFEEYK AHR +AGSKEI+DAEA KSF+ VD DGDGFVDLKEFVELYTMS GEV+VGDIE
Sbjct: 63  SFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDIE 122

Query: 127 SAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSKLMG 186
           SAFKVYDSNGDGKISAEEVM IMKILGE T L AC+QMVKGVD DGDGFID++EFSKLM 
Sbjct: 123 SAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLMD 182

Query: 187 SYSFKFQ 193
           +YS KFQ
Sbjct: 183 TYSSKFQ 189

[Close]

BLAST of Lcy07g004240 vs. NCBI nr
Match: XP_038901754.1 (probable calcium-binding protein CML32 [Benincasa hispida])

HSP 1 Score: 327.0 bits (837), Expect = 1.1e-85
Identity = 168/194 (86.60%), Postives = 181/194 (93.30%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPRSN-SSTSSKHFRPNLEEVKWAFDKFDS 60
           MTNKGRSVLSLFTSC+RKRQ K IP+STQ+K RSN SSTSSKHFRPNLEE+KWAF+KFDS
Sbjct: 1   MTNKGRSVLSLFTSCTRKRQPKNIPSSTQIKQRSNSSSTSSKHFRPNLEEIKWAFEKFDS 60

Query: 61  NKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEV 120
           NKDGKISFEEY+AAHR MAGSKEISD EAAKSF+FVDVDGDGFVDLKEF+ELYTMSGGEV
Sbjct: 61  NKDGKISFEEYQAAHRGMAGSKEISDEEAAKSFKFVDVDGDGFVDLKEFLELYTMSGGEV 120

Query: 121 RVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKE 180
           RVGDI+SAF+VYDSNGDGKISAEEVM IM  LGERT LKAC+QMVKGVD DGDGFID+ E
Sbjct: 121 RVGDIKSAFQVYDSNGDGKISAEEVMAIMNTLGERTTLKACKQMVKGVDIDGDGFIDIDE 180

Query: 181 FSKLMGSYSFKFQN 194
           FSKLMG+YS KFQN
Sbjct: 181 FSKLMGTYSSKFQN 194

[Close]

BLAST of Lcy07g004240 vs. NCBI nr
Match: XP_023532624.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.8 bits (813), Expect = 6.6e-83
Identity = 165/194 (85.05%), Postives = 177/194 (91.24%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLSLFTSCS KRQ+KQIP+STQ K R    SNSSTSSKH R NLEE+KWAFDK
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISNSSTSSKHVRVNLEEIKWAFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAH AM+GSKEISDAEAAKSFEFVD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFEFVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GD++SAFKVYDSNGDGKISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDVQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           + EFS LMG+YSFK
Sbjct: 181 IDEFSSLMGTYSFK 194

[Close]

BLAST of Lcy07g004240 vs. NCBI nr
Match: KAG6605248.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.5 bits (802), Expect = 1.2e-81
Identity = 164/194 (84.54%), Postives = 175/194 (90.21%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLS+FTSCS KRQ+KQIP+STQ K R    S+SS SSKH R NLEE+KWAFDK
Sbjct: 1   MKNKGRSVLSIFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWAFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FDSNKDGKISFEEYKAAH AM+GSKEISDAEAAKSF+ VD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDSNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GDI+SAFKVYDSNGDGKISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           M EFS LMGSYSFK
Sbjct: 181 MDEFSSLMGSYSFK 194

[Close]

BLAST of Lcy07g004240 vs. NCBI nr
Match: XP_022946935.1 (calmodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 310.1 bits (793), Expect = 1.4e-80
Identity = 162/194 (83.51%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLSLFTSCS KRQ+KQIP+STQ K R    S+SS SSKH R NLEE+KW FDK
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAH AM+GSKEISDAEAAKSF+ VD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GDI+SAFKVYDSNGDGKISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           + EFS LMGSYSFK
Sbjct: 181 IDEFSSLMGSYSFK 194

[Close]

BLAST of Lcy07g004240 vs. NCBI nr
Match: XP_023007371.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 307.8 bits (787), Expect = 6.8e-80
Identity = 158/194 (81.44%), Postives = 176/194 (90.72%), Query Frame = 0


Query: 1   MTNKGRSVLSLFTSCSRKRQIKQIPTSTQLKPR----SNSSTSSKHFRPNLEEVKWAFDK 60
           M NKGRSVLSLFTSCS KRQ+KQIP+STQ K R    S+SS SSKH + NLEE+KWAF+K
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRFKSISDSSASSKHVQVNLEEIKWAFEK 60

Query: 61  FDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAHRAM+GS+EISD EAAKSF+FVD DGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHRAMSGSQEISDVEAAKSFKFVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFID 180
           GEV++GD++SAFKVYDSNGDG+ISAEEVMEIMKILGE T LKACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDVQSAFKVYDSNGDGRISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 MKEFSKLMGSYSFK 191
           + EFS LMG+YSFK
Sbjct: 181 IDEFSSLMGTYSFK 194

[Close]

BLAST of Lcy07g004240 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.1e-33
Identity = 80/184 (43.48%), Postives = 117/184 (63.59%), Query Frame = 0


Query: 7   SVLSLFTSCSRKRQIKQIPTSTQLKPRSNSSTSSK----HFRPNLEEVKWAFDKFDSNKD 66
           S L L    S+ + +++ P+    + R +S  SS       +P++ E++  F +FD +KD
Sbjct: 5   SFLELQYKLSKNKMLRK-PSRMFSRDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKD 64

Query: 67  GKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYTMSGGEVRVG 126
           GKIS  EYK   RA+   + I D    K F+ VD+DGDGF+D +EF++ Y  SGG +R  
Sbjct: 65  GKISQTEYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSS 124

Query: 127 DIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDTDGDGFIDMKEFSK 186
           DI ++F  +D NGDGKISAEEVM ++  LGER +L+ C +MV+ VD DGDG ++M+EF K
Sbjct: 125 DIRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIK 184

[Close]

BLAST of Lcy07g004240 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 112.5 bits (280), Expect = 3.9e-25
Identity = 60/146 (41.10%), Postives = 95/146 (65.07%), Query Frame = 0


Query: 46  NLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDL 105
           +++++K  F +FD N DGKIS +E K   RA+  S   S  E     +  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 106 KEFVELYTM---SGGEVR--VGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKAC 165
            EFV L+ +    GG  R  V D++ AF++YD +G+G+ISA+E+  +MK LGE+ +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 166 RQMVKGVDTDGDGFIDMKEFSKLMGS 187
           ++M+  VD DGDG ++  EF K+M +
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMMSN 157

[Close]

BLAST of Lcy07g004240 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 109.8 bits (273), Expect = 2.5e-24
Identity = 59/152 (38.82%), Postives = 94/152 (61.84%), Query Frame = 0


Query: 36  SSTSSKHFRPNLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFV 95
           S   S++   ++E++K  F +FD N DGKIS +E K    A+  S   S  E     +  
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEF 61

Query: 96  DVDGDGFVDLKEFVELYTM---SGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILG 155
           D+DG+GF+DL EFV L+ +   S     + D++ AF +YD + +G+ISA E+  +MK LG
Sbjct: 62  DLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG 121

Query: 156 ERTNLKACRQMVKGVDTDGDGFIDMKEFSKLM 185
           E+ +++ C++M+  VD+DGDG +D +EF K+M
Sbjct: 122 EKCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Lcy07g004240 vs. TAIR 10
Match: AT5G17470.1 (EF hand calcium-binding protein family )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 60/143 (41.96%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 54  FDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFVDLKEFVELYT 113
           F++ D NKDGKIS++E+  A RA   S  I+  E    F  +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIRAF--SPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 114 MSGGEVRVGD----IESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACRQMVKGVDT 173
           M GGE    D    ++ AF +YD +GDGKISA E+  ++K LGE+  +  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 174 DGDGFIDMKEFSKLMGSYSFKFQ 193
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of Lcy07g004240 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-22
Identity = 58/143 (40.56%), Postives = 91/143 (63.64%), Query Frame = 0


Query: 44  RPNLEEVKWAFDKFDSNKDGKISFEEYKAAHRAMAGSKEISDAEAAKSFEFVDVDGDGFV 103
           +  + E++  F KFD N DGKIS +E  A   ++    E+ + E  K+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 104 DLKEFVELYT--MSGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMKILGERTNLKACR 163
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+ E+++ LG+  ++  CR
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 164 QMVKGVDTDGDGFIDMKEFSKLM 185
           +M+ GVD DGDG ID +EF  +M
Sbjct: 152 KMIGGVDKDGDGTIDFEEFKIMM 171

The following BLAST results are available for this feature:

BLAST of Lcy07g004240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	7.2e-32	43.48	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
P25070	5.5e-24	41.10	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9C8Y1	3.6e-23	38.82	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9LF55	2.0e-21	41.96	Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 ...	[more]
Q9FYK2	2.6e-21	40.56	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]

BLAST of Lcy07g004240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G5E9	6.7e-81	83.51	calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=...	[more]
A0A6J1L0C4	3.3e-80	81.44	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1	[more]
A0A6J1D869	5.3e-78	83.15	calmodulin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111017929 PE=4 SV...	[more]
A0A0A0LMP3	1.9e-75	80.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1	[more]
A0A5D3BJJ3	3.5e-74	80.21	Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Lcy07g004240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901754.1	1.1e-85	86.60	probable calcium-binding protein CML32 [Benincasa hispida]	[more]
XP_023532624.1	6.6e-83	85.05	calmodulin-like protein 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6605248.1	1.2e-81	84.54	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946935.1	1.4e-80	83.51	calmodulin-like protein 1 [Cucurbita moschata]	[more]
XP_023007371.1	6.8e-80	81.44	calmodulin-like protein 1 [Cucurbita maxima]	[more]

BLAST of Lcy07g004240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	5.1e-33	43.48	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	3.9e-25	41.10	EF hand calcium-binding protein family	[more]
AT1G66400.1	2.5e-24	38.82	calmodulin like 23	[more]
AT5G17470.1	1.4e-22	41.96	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.8e-22	40.56	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 159..187 e-value: 0.073 score: 22.2 coord: 123..151 e-value: 3.8E-6 score: 36.4 coord: 49..77 e-value: 7.4E-7 score: 38.8 coord: 87..115 e-value: 0.0022 score: 27.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 48..112 e-value: 2.7E-12 score: 46.9 coord: 124..184 e-value: 1.4E-11 score: 44.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 155..190 score: 12.253878
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 119..154 score: 15.127104
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 45..80 score: 15.629221
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 83..118 score: 13.397589
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 123..184 e-value: 1.66061E-17 score: 71.0397
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 49..111 e-value: 1.4855E-14 score: 63.3357
None	No IPR available	GENE3D	1.10.238.10		coord: 120..190 e-value: 1.0E-18 score: 69.9
None	No IPR available	GENE3D	1.10.238.10		coord: 12..119 e-value: 1.0E-19 score: 72.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..42
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 12..188
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 12..188
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 58..70
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 168..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 96..108
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 132..144
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 47..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g004240.1	Lcy07g004240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding