Lcy07g002640 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g002640
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationChr07: 10356986 .. 10358867 (-)
RNA-Seq ExpressionLcy07g002640
SyntenyLcy07g002640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAGAGGAGATCAATCCTCAAGTACGGCCCAAAATGGGAAGAACTCTGCAAACTATTATCTTGTTAGTATTAGTTTGTGTGTTTTCTTCTCTTTCTGCTTATTCCTTGCCTTTGTCGACAAAAGGAAGATGGATTGTTGATTCAAAAACAGGGCATCGAGTCAAGCTTGTGTGTGCGAATTGGCCTGCCCACACCCAAAGCATGCTAGCAGAAGGCCTTAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATCAAGTTAAAGTTCAATTGTGTGCGGCTCACATATGCAACCCACATGTTCACTCGCTACGCTAATAGGACAATTGAAGAGAACTTCGACCTTCTTGATTTAAAACAAGCCAAAGCTGGATTAGCCCAATATAACCCTTTTGTGTTGAATAAGACGATCGTTGAAGCCTATGAAGCTGTTGTTGATATGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAACCGAGATGGTGTTGCTCTCTTGATGATGGTAATGGCTTCTTCGGAAATCGAAATTTTGACCCTCAAGAATGGTTACAGGGTCTTCAGTTAGTCGCTAAACGTTTTCTCAACAAACCAACGGTAACATATTCTTGAAACTAAGCATGAGCATTTGATGACTGGAAACAAAATTATTTCATTAATAAATTTTTATTTTGACATATAGGTGGTAGGAATGAGCCTACGAAATGAGATACGAGGAACGATGGAAAATGCGAATGATTGGAACAAGTATATAACTCAAGGAGTAACAACAATCCACAACGCAAATCCAGAAGTTTTAGTGATTGTGTCAGGTCTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCCTTGAATGTTGGCACATTAGACAATAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAGAGCAAATTTGTTCAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTTATAGACCATGCTGAGTTTGTGATAGAAGGATCAAACCCATTTCCTTTGTTTGTTAGCGAATACGGGTACGATCAAACGGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCAAAAAGACTTGGATTGGGCACTATGGACTTGGCAAGGTAGCTATTACTATAGAGAAGGTCAAGCAGAGCCTGCAGAAACATTTGGTGTTCTAAACTCTAATTGGACCCAAATTAAGAATCCCAATTTTCCTAAGAAGTTTCAACTATTGCAGACCATGTTACAAGGTAATTAAATTAAATTTATGATTGTGAATATATTTAAGGATTATTTAAAAGAAAACTGGTTATATATGAAAATTTCCAAGCAACAAAACAACTAAACTAGTTTTTGTAAGATTAATATATAATTCTTTAACCATTTTATGATACGCAGATCCAAATTCCAATGCTTCATCTTCGTATGTTATGTATCATCCACAAAGTGGCCAATGTGTCCAAGCCTCAAATGACAATACTGGAATTTTTTTGAGCAATTGCTCCACCTCAAGTCGTTGGAATCATGACAAAGATGGTGCCCCAATTAGGATGACATCCAATGGTTTGTGTTTGAAGGCCAATGGAAAAGGCCTGGGAGCATCCCTCTCAAGTGACTGCTTAGGTCAACAGAGTGCTTGGAGAGCCATTTCTAACTCTAAGCTTCACCTGGCCGTCTTCACCCAAGATGGGAAGAGCCTTTGCTTACAGATTGAAAATTCCAAGTCTTCAAAAATTGTGGTAAGCTCTTGTATTTGTACCAATGGCGATTCAAATTGTCTCCAAGACACCCAAAGTCAATGGTTCACACTTGTTCCAACCAATACCTTGTAA

mRNA sequence

GAAAAGAGGAGATCAATCCTCAAGTACGGCCCAAAATGGGAAGAACTCTGCAAACTATTATCTTGTTAGTATTAGTTTGTGTGTTTTCTTCTCTTTCTGCTTATTCCTTGCCTTTGTCGACAAAAGGAAGATGGATTGTTGATTCAAAAACAGGGCATCGAGTCAAGCTTGTGTGTGCGAATTGGCCTGCCCACACCCAAAGCATGCTAGCAGAAGGCCTTAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATCAAGTTAAAGTTCAATTGTGTGCGGCTCACATATGCAACCCACATGTTCACTCGCTACGCTAATAGGACAATTGAAGAGAACTTCGACCTTCTTGATTTAAAACAAGCCAAAGCTGGATTAGCCCAATATAACCCTTTTGTGTTGAATAAGACGATCGTTGAAGCCTATGAAGCTGTTGTTGATATGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAACCGAGATGGTGTTGCTCTCTTGATGATGGTAATGGCTTCTTCGGAAATCGAAATTTTGACCCTCAAGAATGGTTACAGGGTCTTCAGTTAGTCGCTAAACGTTTTCTCAACAAACCAACGGTGGTAGGAATGAGCCTACGAAATGAGATACGAGGAACGATGGAAAATGCGAATGATTGGAACAAGTATATAACTCAAGGAGTAACAACAATCCACAACGCAAATCCAGAAGTTTTAGTGATTGTGTCAGGTCTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCCTTGAATGTTGGCACATTAGACAATAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAGAGCAAATTTGTTCAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTTATAGACCATGCTGAGTTTGTGATAGAAGGATCAAACCCATTTCCTTTGTTTGTTAGCGAATACGGGTACGATCAAACGGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCAAAAAGACTTGGATTGGGCACTATGGACTTGGCAAGGTAGCTATTACTATAGAGAAGGTCAAGCAGAGCCTGCAGAAACATTTGGTGTTCTAAACTCTAATTGGACCCAAATTAAGAATCCCAATTTTCCTAAGAAGTTTCAACTATTGCAGACCATGTTACAAGATCCAAATTCCAATGCTTCATCTTCGTATGTTATGTATCATCCACAAAGTGGCCAATGTGTCCAAGCCTCAAATGACAATACTGGAATTTTTTTGAGCAATTGCTCCACCTCAAGTCGTTGGAATCATGACAAAGATGGTGCCCCAATTAGGATGACATCCAATGGTTTGTGTTTGAAGGCCAATGGAAAAGGCCTGGGAGCATCCCTCTCAAGTGACTGCTTAGGTCAACAGAGTGCTTGGAGAGCCATTTCTAACTCTAAGCTTCACCTGGCCGTCTTCACCCAAGATGGGAAGAGCCTTTGCTTACAGATTGAAAATTCCAAGTCTTCAAAAATTGTGGTAAGCTCTTGTATTTGTACCAATGGCGATTCAAATTGTCTCCAAGACACCCAAAGTCAATGGTTCACACTTGTTCCAACCAATACCTTGTAA

Coding sequence (CDS)

ATGGGAAGAACTCTGCAAACTATTATCTTGTTAGTATTAGTTTGTGTGTTTTCTTCTCTTTCTGCTTATTCCTTGCCTTTGTCGACAAAAGGAAGATGGATTGTTGATTCAAAAACAGGGCATCGAGTCAAGCTTGTGTGTGCGAATTGGCCTGCCCACACCCAAAGCATGCTAGCAGAAGGCCTTAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATCAAGTTAAAGTTCAATTGTGTGCGGCTCACATATGCAACCCACATGTTCACTCGCTACGCTAATAGGACAATTGAAGAGAACTTCGACCTTCTTGATTTAAAACAAGCCAAAGCTGGATTAGCCCAATATAACCCTTTTGTGTTGAATAAGACGATCGTTGAAGCCTATGAAGCTGTTGTTGATATGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAACCGAGATGGTGTTGCTCTCTTGATGATGGTAATGGCTTCTTCGGAAATCGAAATTTTGACCCTCAAGAATGGTTACAGGGTCTTCAGTTAGTCGCTAAACGTTTTCTCAACAAACCAACGGTGGTAGGAATGAGCCTACGAAATGAGATACGAGGAACGATGGAAAATGCGAATGATTGGAACAAGTATATAACTCAAGGAGTAACAACAATCCACAACGCAAATCCAGAAGTTTTAGTGATTGTGTCAGGTCTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCCTTGAATGTTGGCACATTAGACAATAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAGAGCAAATTTGTTCAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTTATAGACCATGCTGAGTTTGTGATAGAAGGATCAAACCCATTTCCTTTGTTTGTTAGCGAATACGGGTACGATCAAACGGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCAAAAAGACTTGGATTGGGCACTATGGACTTGGCAAGGTAGCTATTACTATAGAGAAGGTCAAGCAGAGCCTGCAGAAACATTTGGTGTTCTAAACTCTAATTGGACCCAAATTAAGAATCCCAATTTTCCTAAGAAGTTTCAACTATTGCAGACCATGTTACAAGATCCAAATTCCAATGCTTCATCTTCGTATGTTATGTATCATCCACAAAGTGGCCAATGTGTCCAAGCCTCAAATGACAATACTGGAATTTTTTTGAGCAATTGCTCCACCTCAAGTCGTTGGAATCATGACAAAGATGGTGCCCCAATTAGGATGACATCCAATGGTTTGTGTTTGAAGGCCAATGGAAAAGGCCTGGGAGCATCCCTCTCAAGTGACTGCTTAGGTCAACAGAGTGCTTGGAGAGCCATTTCTAACTCTAAGCTTCACCTGGCCGTCTTCACCCAAGATGGGAAGAGCCTTTGCTTACAGATTGAAAATTCCAAGTCTTCAAAAATTGTGGTAAGCTCTTGTATTTGTACCAATGGCGATTCAAATTGTCTCCAAGACACCCAAAGTCAATGGTTCACACTTGTTCCAACCAATACCTTGTAA

Protein sequence

MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSKLHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL
Homology
BLAST of Lcy07g002640 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 6.2e-118
Identity = 231/546 (42.31%), Postives = 329/546 (60.26%), Query Frame = 0

Query: 5   LQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGLNH 64
           L T +LL+LV    +  ++SLPL T+GRWIVD  TG RVKL C NW  H +  L EGLN 
Sbjct: 13  LLTALLLLLV----AAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNR 72

Query: 65  RPLKELADEAIKLKFNCVRLTYATHMFTR--YANRTIEENFDLLDLKQAKAGLAQYNPFV 124
            P+  +A     L FNCVRLTY+ HM TR  Y N T+ + F  L+L +A +G+   NP +
Sbjct: 73  LPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPEL 132

Query: 125 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 184
           L+   V AY  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+R F+P  W++GL 
Sbjct: 133 LDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLG 192

Query: 185 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 244
           L+A  F N P VV MSLRNE+RG       W++++  G  T+H ANP+VLVI+SGL +D 
Sbjct: 193 LMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDT 252

Query: 245 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 304
           DL  L   P+ +     K+V+E H YSF     +       N++C N    F  +  FV 
Sbjct: 253 DLSFLPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVT 312

Query: 305 EGSN--PFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQ- 364
             +N    PLF+SE+G DQ  VND +NR+++C +A+LA++DLDWALWT  GSYYYR  + 
Sbjct: 313 SSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQ 372

Query: 365 --AEPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSY-VMYHPQSGQCVQA 424
              +  ET+G  N +W++I+NP+F  + + +Q  +QDP       Y ++YHP SG CV++
Sbjct: 373 PVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVES 432

Query: 425 SNDNTGIFLSNC-STSSRWNHDKD-GAPIRMTSNGLCLKANGKGLGASLSSDCLG-QQSA 484
              NT + L +C S  SRWN+D     PI +  +  C+   G GL A ++ +C     + 
Sbjct: 433 GIGNT-VHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTL 492

Query: 485 WRAISNSKLHLA--VFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQ--DTQSQWF 536
           W  +S+++L L   V  +DGK   + ++ SKS  I  + CIC   DS+C    + + QWF
Sbjct: 493 WSTVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWF 547

BLAST of Lcy07g002640 vs. ExPASy Swiss-Prot
Match: P54583 (Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0614 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.9e-16
Identity = 84/366 (22.95%), Postives = 150/366 (40.98%), Query Frame = 0

Query: 29  TKGRWIVDSKTGHRVKLVCANW-PAHTQSMLAEGLNHRPLKELADEAIKLKFNCVRLTYA 88
           T GR I+D+     V++   NW    T + +  GL  R  + + D+   L +N +RL Y+
Sbjct: 49  TSGREILDA-NNVPVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYS 108

Query: 89  THMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIVEAYEAVVDMLGASGLMVIA 148
             +       T+  + +   + Q   GL          T ++  + +V   G  GL +I 
Sbjct: 109 DDIL---KPGTMPNSINFYQMNQDLQGL----------TSLQVMDKIVAYAGQIGLRIIL 168

Query: 149 DNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQLVAKRFLNKPTVVGMSLRNEIR--- 208
           D H    R  CS    +  +   +     W+  LQ +A+R+   PTVVG  L NE     
Sbjct: 169 DRH----RPDCS--GQSALWYTSSVSEATWISDLQALAQRYKGNPTVVGFDLHNEPHDPA 228

Query: 209 --GTMENANDWNKYITQGVTTIHNANPEVLVIVSGL-NYDND-------LRCLKEKPLNV 268
             G  + + DW     +    + + NP +L+ V G+ +Y+ D       L+   + P+ V
Sbjct: 229 CWGCGDPSIDWRLAAERAGNAVLSVNPNLLIFVEGVQSYNGDSYWWGGNLQGAGQYPV-V 288

Query: 269 GTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSE 328
             + N+LV+  H Y+ S   ++ F      N    I N    +    +   N  P+++ E
Sbjct: 289 LNVPNRLVYSAHDYATSVYPQTWFSDPTFPNNMPGIWNKNWGY----LFNQNIAPVWLGE 348

Query: 329 YGYD-QTEVNDAENRFMSCFMAHLAQKDLD---WALWTWQGSYYYREGQAEPAETFGVLN 377
           +G   Q+  +    + +  ++   AQ   D   W  W+W           +  +T G+L 
Sbjct: 349 FGTTLQSTTDQTWLKTLVQYLRPTAQYGADSFQWTFWSW---------NPDSGDTGGILK 380

BLAST of Lcy07g002640 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 2.9e-14
Identity = 100/413 (24.21%), Postives = 167/413 (40.44%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCAN-WPAHTQSMLAEG 62
           RT  T+ L   + + +   A+S  ++   R IVD  +G  V+L   N +   T + +  G
Sbjct: 5   RTASTLALATALALAAG-PAFSYSIN-NSRQIVDD-SGKVVQLKGVNVFGFETGNHVMHG 64

Query: 63  LNHRPLKELADEAIKLKFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKAGLAQY 122
           L  R  K++  +   L FN VRL +        T  A+     N DL  L   +      
Sbjct: 65  LWARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ------ 124

Query: 123 NPFVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWL 182
              +L+K I E          A G+ V+ D+H      C  + +    +   ++   +WL
Sbjct: 125 ---ILDKVIAE--------FNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWL 184

Query: 183 QGLQLVAKRFLNKPTVVGMSLRNEIR-----GTMENANDWNKYITQGVTTIHNANPEVLV 242
             L+ VA R+ N P V+G+ L+NE       GT   A DWNK   +G   +    P+ L+
Sbjct: 185 ADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLI 244

Query: 243 IVSGLN------------YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQ 302
            V G+             +  +L+ L   PLN+    N+L+   H+Y         FVQ 
Sbjct: 245 AVEGITDNPVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVY-----GPDVFVQS 304

Query: 303 PLNNICANIMNGF--IDHAEFVIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQ 362
             N+  +N  N    I    F  + +    L + E+G    E +  +  +    + +L  
Sbjct: 305 YFND--SNFPNNMPAIWERHFG-QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRS 364

Query: 363 KDLDWAL-WTWQGSYYYREGQAEPAETFGVLNSNWTQIKNPNFPKKFQLLQTM 392
           K ++    W+W              +T G+L  +WT ++      K  LL+T+
Sbjct: 365 KGINQGFYWSW---------NPNSGDTGGILRDDWTSVRQ----DKMTLLRTL 368

BLAST of Lcy07g002640 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.6e-271
Identity = 450/535 (84.11%), Postives = 486/535 (90.84%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGL 62
           RT  TI+L+ LV VFS LS  SLPLST+GRWI+DS+TG RVKLVC NWP+HTQSML EGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 122
           NHRPLK+LADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 182
           LNKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL 
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 242
           LVA+RF NK TVVGMSLRNEIRG MENANDWNKYITQGVTTIHN N EVLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 302
           DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEP 362
           +GSNPFPLFVSE+GYDQ EVNDAENRFMSCF AHLA +DLDWALW WQGSYY+REGQAEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTG 422
            E+FGVL+SNWTQ+KNPNF KKFQLLQTMLQDPNSN S SYV+YHPQS QC+Q SNDN  
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSKL 482
           IFL++CST +RW+H  D  PI+M+S GLCLKA+G+GL ASLS+DCL QQS W AISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           HLA  TQ GKSLCLQI++S SSK+V +SCICTNGD NCLQDT+SQWF LV TNTL
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of Lcy07g002640 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 8.1e-270
Identity = 449/535 (83.93%), Postives = 484/535 (90.47%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGL 62
           RT  TI+LL LV VFS LS  SLPLST+GRWI+D +TG RVKLVC NWP+HTQSML EGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 122
           NHRPLK+LADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 182
           LNKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL 
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 242
           LVA+RF NK TVVGMSLRNEIRG MENANDWNKYITQGVTTIHN N EVLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 302
           DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEP 362
           +GSNPFPLFVSE+GYDQ EVNDAENRFMSCF AHLA +DLDWALW WQGSYY+REGQAEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTG 422
            E+FGVL+SNWTQ+KNPNF KKFQLLQTMLQDPNSNAS SYV+YHPQ+ QC+Q SNDN  
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 423 IFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSKL 482
           IFL++CST +RW+H  D  PI+M+S GLCLKA+G+GL ASLS+DCL QQS W AISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 483 HLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           HLA   Q G SLCLQI++S SSK+V +SCICTNGD NCLQDT+SQWF LV TNTL
Sbjct: 482 HLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of Lcy07g002640 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.2e-268
Identity = 450/538 (83.64%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           M RT+Q I+LL LV VFSS SAYSLPLST GRWI+DS++G RVKLVC NWP+HTQSML E
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNP 120
           GLNHRPLKELADEAIKL+FNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 FVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           FVLNKTI EAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNR FDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNY 240
           L LVA+RF NK TVVGMSLRNE+RG MENANDWN Y+TQGVTTIH  NP VLVIVSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQ EV+DAENRFMSCF AHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDN 420
           E AETFGVL+SNWTQIKNPNF +KFQLLQTMLQDP SNAS SYV+YH QSGQC++ SNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 TGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNS 480
             IFL+NCSTSSRW+HD D  PI+M+S GLCLKA+G+GL ASLS+DC+G+QS W AISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLAVFTQDGKSLCLQ-IENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
            LHL   T+DGKSLCLQ IE+S SSKIV +SCICT  D  CLQDTQSQWF LV TNTL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of Lcy07g002640 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.6e-268
Identity = 449/536 (83.77%), Postives = 484/536 (90.30%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGL 62
           RT   I+LL LV VFS L A SLPLST+GRWI+DS+TG RVKLVC NWP+HTQSML EGL
Sbjct: 5   RTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 64

Query: 63  NHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 122
           NHRPLK+LADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA YNPFV
Sbjct: 65  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFV 124

Query: 123 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 182
           LNKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL 
Sbjct: 125 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 184

Query: 183 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 242
           LVA+RF NK TVVGMSLRNEIRG MENANDWNKYITQGVTTIHN N EVLVIVSGLNYDN
Sbjct: 185 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 244

Query: 243 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 302
           DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV+
Sbjct: 245 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 304

Query: 303 EGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEP 362
           +GSNPFPLFVSE+GYDQ EVNDAENRFMSCF AHLA +DLDWALW WQGSYY+REGQAEP
Sbjct: 305 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 364

Query: 363 AETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTG 422
            E+FGVL+SNWTQ+KNPNF KKFQLLQTMLQDPNSNAS SYV+YHPQS QC+Q SNDN  
Sbjct: 365 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNKE 424

Query: 423 IFLSNC-STSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSK 482
           IFL++C ST +RW+H  D  PI+M+S GLCLKA+G+GL ASLS+DCL QQS W AISNSK
Sbjct: 425 IFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSK 484

Query: 483 LHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           LHLA  TQ GKSLCLQI++S  SK+V +SCICTNGD NCLQDT+SQWF LV TNTL
Sbjct: 485 LHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of Lcy07g002640 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.4e-266
Identity = 445/537 (82.87%), Postives = 474/537 (88.27%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           MGR  Q +  L L  VF S   YSLPLSTK +WI+DS TG RVKLVC NWP+HTQSML E
Sbjct: 1   MGRIFQVVFFLALF-VFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNP 120
           GLNHRPLKELADEAIKL+FNCVRLTYAT MFTRYANRT+EENFDLLDL+QAKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 121 FVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           FVLNKTI EAYEAVVD+LG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNY 240
           L LVA+RF  K TVVGMSLRNEIRGT ENANDWN Y+TQGVTTIHN NP VLVIVSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLKEKPLNV TLDNKLVFEVHLYSFSGD ESKF+ QPLNNICANI+NGF+DHAEF
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQA 360
           V EGSNPFPLFVSEYGYDQ EVNDAENRFMSCF AHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDN 420
           EP ETFGVL+SNWTQIKNPNF +KFQLLQTMLQDPNSNAS SYV+YHPQSGQC+Q SNDN
Sbjct: 361 EPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 TGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNS 480
             IF+ NCS SSRW HD D  PIRM+S GLCLK  G+GL  SLS+DCLG QS+W AISN+
Sbjct: 421 KDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNT 480

Query: 481 KLHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           KLHLA  +QDGKSLCLQ+E+S SSKIV +SCICTNG  NCLQDT+SQWF LV TNTL
Sbjct: 481 KLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

BLAST of Lcy07g002640 vs. NCBI nr
Match: XP_038900453.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.2e-289
Identity = 481/537 (89.57%), Postives = 505/537 (94.04%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           MGR LQTI+ LVLV  F   SAYSLPLSTKGRWIVDSKTGHRVK VCANWP+HTQSMLAE
Sbjct: 1   MGRNLQTIVFLVLVFEFLFFSAYSLPLSTKGRWIVDSKTGHRVKFVCANWPSHTQSMLAE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNP 120
           GLNHRPLKELADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQ+NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQHNP 120

Query: 121 FVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           F+LNKTIVEAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG
Sbjct: 121 FLLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180

Query: 181 LQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNY 240
           L+LVAKRF+NK TVV MSLRNEIRGTMENANDWN Y+TQGVTTIHN NPEVLVIVSGLNY
Sbjct: 181 LRLVAKRFVNKSTVVAMSLRNEIRGTMENANDWNNYVTQGVTTIHNINPEVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV QPLN+ICA IMNGF+DHA F
Sbjct: 241 DNDLRCLKEKPLNVNTLDNKLVFEVHLYSFSGDSESKFVTQPLNDICATIMNGFVDHAGF 300

Query: 301 VIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEGSNPFPLFVSEYGYDQ EVN+AENRFMSCF AHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGSNPFPLFVSEYGYDQREVNEAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDN 420
           EPAETFGVL+SNWTQIKNPNFPKKFQLLQTMLQDPNSNAS+SYVMYHPQSGQCVQA +DN
Sbjct: 361 EPAETFGVLDSNWTQIKNPNFPKKFQLLQTMLQDPNSNASTSYVMYHPQSGQCVQALDDN 420

Query: 421 TGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNS 480
            G+FL+NCST SRW+H+ DG+PIR+ S GLCLKANG+GLGASLSSDCL QQSAWRAISNS
Sbjct: 421 KGMFLNNCSTPSRWSHNGDGSPIRLRSTGLCLKANGEGLGASLSSDCLSQQSAWRAISNS 480

Query: 481 KLHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           KLHLA F QDGKSLCLQ+ENS SSKIVV+SCICTNGD  CLQDTQSQW TLV TNTL
Sbjct: 481 KLHLATFAQDGKSLCLQLENSNSSKIVVNSCICTNGDPKCLQDTQSQWLTLVATNTL 537

BLAST of Lcy07g002640 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 943.0 bits (2436), Expect = 1.2e-270
Identity = 450/535 (84.11%), Postives = 486/535 (90.84%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGL 62
           RT  TI+L+ LV VFS LS  SLPLST+GRWI+DS+TG RVKLVC NWP+HTQSML EGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 122
           NHRPLK+LADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 182
           LNKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL 
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 242
           LVA+RF NK TVVGMSLRNEIRG MENANDWNKYITQGVTTIHN N EVLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 302
           DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEP 362
           +GSNPFPLFVSE+GYDQ EVNDAENRFMSCF AHLA +DLDWALW WQGSYY+REGQAEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTG 422
            E+FGVL+SNWTQ+KNPNF KKFQLLQTMLQDPNSN S SYV+YHPQS QC+Q SNDN  
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSKL 482
           IFL++CST +RW+H  D  PI+M+S GLCLKA+G+GL ASLS+DCL QQS W AISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           HLA  TQ GKSLCLQI++S SSK+V +SCICTNGD NCLQDT+SQWF LV TNTL
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of Lcy07g002640 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 939.1 bits (2426), Expect = 1.7e-269
Identity = 449/535 (83.93%), Postives = 484/535 (90.47%), Query Frame = 0

Query: 3   RTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGL 62
           RT  TI+LL LV VFS LS  SLPLST+GRWI+D +TG RVKLVC NWP+HTQSML EGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 122
           NHRPLK+LADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQ 182
           LNKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL 
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDN 242
           LVA+RF NK TVVGMSLRNEIRG MENANDWNKYITQGVTTIHN N EVLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVI 302
           DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEP 362
           +GSNPFPLFVSE+GYDQ EVNDAENRFMSCF AHLA +DLDWALW WQGSYY+REGQAEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTG 422
            E+FGVL+SNWTQ+KNPNF KKFQLLQTMLQDPNSNAS SYV+YHPQ+ QC+Q SNDN  
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 423 IFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNSKL 482
           IFL++CST +RW+H  D  PI+M+S GLCLKA+G+GL ASLS+DCL QQS W AISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 483 HLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
           HLA   Q G SLCLQI++S SSK+V +SCICTNGD NCLQDT+SQWF LV TNTL
Sbjct: 482 HLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of Lcy07g002640 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 935.3 bits (2416), Expect = 2.4e-268
Identity = 450/538 (83.64%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           M RT+Q I+LL LV VFSS SAYSLPLST GRWI+DS++G RVKLVC NWP+HTQSML E
Sbjct: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNP 120
           GLNHRPLKELADEAIKL+FNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNP
Sbjct: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148

Query: 121 FVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           FVLNKTI EAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNR FDPQEWLQG
Sbjct: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208

Query: 181 LQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNY 240
           L LVA+RF NK TVVGMSLRNE+RG MENANDWN Y+TQGVTTIH  NP VLVIVSGLNY
Sbjct: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEF
Sbjct: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328

Query: 301 VIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQ EV+DAENRFMSCF AHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388

Query: 361 EPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDN 420
           E AETFGVL+SNWTQIKNPNF +KFQLLQTMLQDP SNAS SYV+YH QSGQC++ SNDN
Sbjct: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448

Query: 421 TGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNS 480
             IFL+NCSTSSRW+HD D  PI+M+S GLCLKA+G+GL ASLS+DC+G+QS W AISNS
Sbjct: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508

Query: 481 KLHLAVFTQDGKSLCLQ-IENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
            LHL   T+DGKSLCLQ IE+S SSKIV +SCICT  D  CLQDTQSQWF LV TNTL
Sbjct: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 566

BLAST of Lcy07g002640 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 935.3 bits (2416), Expect = 2.4e-268
Identity = 450/538 (83.64%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           M RT+Q I+LL LV VFSS SAYSLPLST GRWI+DS++G RVKLVC NWP+HTQSML E
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNP 120
           GLNHRPLKELADEAIKL+FNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 FVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           FVLNKTI EAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNR FDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNY 240
           L LVA+RF NK TVVGMSLRNE+RG MENANDWN Y+TQGVTTIH  NP VLVIVSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQ EV+DAENRFMSCF AHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDN 420
           E AETFGVL+SNWTQIKNPNF +KFQLLQTMLQDP SNAS SYV+YH QSGQC++ SNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 TGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKANGKGLGASLSSDCLGQQSAWRAISNS 480
             IFL+NCSTSSRW+HD D  PI+M+S GLCLKA+G+GL ASLS+DC+G+QS W AISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLAVFTQDGKSLCLQ-IENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLVPTNTL 538
            LHL   T+DGKSLCLQ IE+S SSKIV +SCICT  D  CLQDTQSQWF LV TNTL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of Lcy07g002640 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 406.4 bits (1043), Expect = 3.6e-113
Identity = 207/514 (40.27%), Postives = 316/514 (61.48%), Query Frame = 0

Query: 24  SLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGLNHRPLKELADEAIKLKFNCVR 83
           S PLST  RWIVD + G RVKLVCANWP+H Q ++AEGL+ +P+  +A + +++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 84  LTYATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIVEAYEAVVDMLGA 143
           LT+   + T      N T+ ++F  L L     G    NP +++  ++EAY+ VV  LG 
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 144 SGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQLVAKRFLNKPTVVGMSLR 203
           + +MVI DNH+++P WCC+ DDGNGFFG++ FDP  W+  L+ +A  F     VVGMSLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 204 NEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNK 263
           NE+RG  +N NDW KY+ QG   +H+AN +VLVI+SGL++D DL  ++ +P+ + +   K
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKL-SFTGK 271

Query: 264 LVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQT 323
           LVFE+H YSFS D  S     P N+IC  ++N   +   +++  +  FPLF+SE+G D+ 
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLL--NQGFPLFLSEFGIDER 331

Query: 324 EVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEPAETFGVLNSNWTQIKNPN 383
            VN  +NR+  C     A+ D+DW+LW   GSYY R+G+    E +GVL+S+W  ++N +
Sbjct: 332 GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSS 391

Query: 384 FPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTGIF-LSNCSTSSRWNHDKD 443
           F +K   LQ+ LQ P     +  +++HP +G C+  S D+  +  L  C++S  W++ K 
Sbjct: 392 FLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKK 451

Query: 444 GAPIRMTSNGLCLKANG-KGLGASLSSDCLGQQSAWRAISNSKLHLAVFTQDGKSLCLQI 503
              +R+    LCL++NG K       + C    S W+ IS S++HLA  T +  SLCL +
Sbjct: 452 A--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDV 511

Query: 504 ENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLV 533
           +   ++ +V ++C C + D +C  +  SQWF ++
Sbjct: 512 DT--ANNVVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of Lcy07g002640 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 384.8 bits (987), Expect = 1.1e-106
Identity = 210/523 (40.15%), Postives = 303/523 (57.93%), Query Frame = 0

Query: 20  LSAYSLPLSTKGRWIV-DSKTGHRVKLVCANWPAHTQSMLAEGLNHRPLKELADEAIKLK 79
           ++ ++ P ST  RWIV D   G RVKL C NWP+H ++ +AEGL+ +PL  +A++ + + 
Sbjct: 16  ITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIAEKIVSMG 75

Query: 80  FNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIVEAYEAVV 139
           FNCVRLT+  ++ T     A  T+ ++     L +A +G   +NP +L+  +++A++ VV
Sbjct: 76  FNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLIKAFQEVV 135

Query: 140 DMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQLVAKRFLN-KPTV 199
             L    +MVI DNHISQP WCCS +DGNGFFG+++ +PQ W++GL+ +A  F N    V
Sbjct: 136 YCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMFANVSSNV 195

Query: 200 VGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDNDLRCLKEKPLNV 259
           VGMSLRNE+RG  +N  DW KY+ +G   +H+ NP VLVIVSGLNY  DL  L+E+P  V
Sbjct: 196 VGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFLRERPFEV 255

Query: 260 GTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSE 319
            +   K+VFE+H Y F    E       LN IC       +  + F++E     PLFVSE
Sbjct: 256 -SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGFLLE--KGIPLFVSE 315

Query: 320 YGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEPAETFGVLNSNWT 379
           +G DQ   N  +N+F+SCFMA  A +DLDW+LWT  GSYY RE      E++GVL+ NW+
Sbjct: 316 FGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGVLDFNWS 375

Query: 380 QIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCVQASNDNTGIFLSNCSTSSRW 439
            I+N    +    +QT             +M+HP +G C+        + L +C+ S  W
Sbjct: 376 SIRNSTILQMISAIQTPFIG-LMETQPKKIMFHPSTGLCI-VRKSLFQLKLGSCNRSESW 435

Query: 440 NHDKDGAPIRMTSNGLCLKANGKGLGASL-----SSDCLGQQSAWRAISNSKLHLAVFTQ 499
                          LCLKA  KG    L      S C    S W+  S+SK+ L+  T+
Sbjct: 436 RLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYC----SKWKLFSDSKMQLSSITK 495

Query: 500 DGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTLV 533
           +G S+CL ++ ++++ IV +SC C  G+S+C  D +SQWF LV
Sbjct: 496 NGFSVCLDVD-TENNNIVTNSCKCLRGNSSC--DPRSQWFKLV 522

BLAST of Lcy07g002640 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 379.8 bits (974), Expect = 3.6e-105
Identity = 199/514 (38.72%), Postives = 304/514 (59.14%), Query Frame = 0

Query: 26  PLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGLNHRPLKELADEAIKLKFNCVRLT 85
           PLST  RWI+D K G RVKL C NWP+H Q ++AEGL+ + + +LA + + + FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIVEAYEAVVDMLGASG 145
           +   + T      N T+ ++F  L L    +G    NP +++  ++EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQLVAKRFLNKPTVVGMSLRNE 205
           +MVI DNH+++P WCC  +DGNGFFG+  FDP  W+ GL  +A  F     VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 IRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLV 265
           +RG  +N +DW KY+ QG   +H ANP VLVI+SGL+YD DL  ++ + +N+ T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 266 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQTEV 325
           FE+H YSF+  + + +  +  N  C  I+    +   F +     FP+F+SE+G D    
Sbjct: 244 FELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGK 303

Query: 326 NDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEPAETFGVLNSNWTQIKNPNFP 385
           N  +NR++ C +   A+ D+DW++WT QGSYY REG    +E +G+L+S+W ++++ +F 
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 386 KKFQLLQTMLQDPNSNASSSYVMYHPQSGQC-VQASNDNTGIFLSNCSTSSRWNHDKDGA 445
           ++  L+ + LQ P S +    +++HP +G C +Q+  D T + L  C+ S  W++     
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 446 PIRMTSNGLCLKANGKGLGASLS-SDCLGQQ-SAWRAISNSKLHLAVFTQDGKSLCLQIE 505
            + +    LCL++ G      LS + C     S W  IS S + LA       SLCL ++
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAA-KSTNNSLCLDVD 483

Query: 506 NSKSSKIVVSSCICTNG-DSNCLQDTQSQWFTLV 533
             +++ ++ S+C C  G DS+C  D  SQWF +V
Sbjct: 484 --ETNNLMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of Lcy07g002640 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-100
Identity = 192/531 (36.16%), Postives = 306/531 (57.63%), Query Frame = 0

Query: 7   TIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAEGLNHRP 66
           T+ L + + + S   A   PL TK RWIV++K GHRVKL CANWP+H + ++AEGL+ +P
Sbjct: 8   TVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQP 67

Query: 67  LKELADEAIKLKFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKAGLAQYNPFVL 126
           +  ++ +   + FNCVRLT+   +    T   N T++++F+   L     G+  +NP+++
Sbjct: 68  MDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIV 127

Query: 127 NKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLQL 186
           N  ++  ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+ GL+ 
Sbjct: 128 NTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKK 187

Query: 187 VAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSGLNYDND 246
           +A  F+N   VVGMSLRNE+RG    + DW KY+ +G   +H +NP VLVI+SGLN+D D
Sbjct: 188 MATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDAD 247

Query: 247 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 306
           L  LK++P+N+ +   KLV E+H YSF+ D   ++    +N+ C+ + +       FV++
Sbjct: 248 LSFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD 307

Query: 307 GSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGQAEPA 366
               FPLF+SE+G DQ   +   NR+M+C +A  A+KDLDWA+W   G YY+REG+    
Sbjct: 308 --QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVV 367

Query: 367 ETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCV--QASNDNT 426
           E +G+L++NW  + N  + ++  ++Q     P    +    ++HP +G C+  ++    +
Sbjct: 368 EAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHES 427

Query: 427 GIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKA-NGKGLGASLSSDCLGQQSAWRAISNS 486
            + L  C+    W++   G       +  CL+     G    L   C    +    IS +
Sbjct: 428 ELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRIC----TKIEQISAT 487

Query: 487 KLHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFTL 532
           K+HL+  T DG  +CL +++   + +V +SC C  GD+ C  +  SQWF +
Sbjct: 488 KMHLSFNTSDGSLVCLDVDS--DNNVVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Lcy07g002640 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 6.1e-84
Identity = 189/542 (34.87%), Postives = 289/542 (53.32%), Query Frame = 0

Query: 1   MGRTLQTIILLVLVCVFSSLSAYSLPLSTKGRWIVDSKTGHRVKLVCANWPAHTQSMLAE 60
           M R+    +  + +   S L+  S PLSTK RWIVD K G RVKL C NWPAH Q  +AE
Sbjct: 1   MARSFYFCLFFLFISSTSKLTT-SYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQ 120
           GL+ +PL  ++ + + + FNCVRLT+   + T        T++++F+ L L +   G+  
Sbjct: 61  GLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQT 120

Query: 121 YNPFVLNKTIVEAYEAVVDMLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEW 180
           +NP +L+  +  A++ VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W
Sbjct: 121 HNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVW 180

Query: 181 LQGLQLVAKRFLNKPTVVGMSLRNEIRGTMENANDWNKYITQGVTTIHNANPEVLVIVSG 240
            +GL+ +A  F N   V+GMSLRNE RG  +  + W +++ QG   +H ANP++LVI+SG
Sbjct: 181 AKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSG 240

Query: 241 LNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDH 300
           +++D +L  L+++ +NV   D KLVFE+H YSFS D    + +   N+ C  I+     +
Sbjct: 241 IDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHN 300

Query: 301 AEFVIEGSNPFPLFVSEYGYDQTEVNDAENRFMSCFMAHLAQKDLDWALWTWQGSYYYRE 360
             F++     FPL +SE+G DQ   + + NR+M+C +A  A+ DLDWA+W   G YY R 
Sbjct: 301 GGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRT 360

Query: 361 GQAEPAETFGVLNSNWTQIKNPNFPKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCV--Q 420
           G                                    PN N     +++HP +G CV   
Sbjct: 361 GPG--------------------------------LRPNKN-----LLFHPSTGLCVTNN 420

Query: 421 ASNDNTGIFLSNCSTSSRWNHDKDGAPIRMTSNGLCLKA-NGKG----LGASLSSDCLGQ 480
            S++   + L  C  S  W  +     + +  N +C++A N  G    LG       LGQ
Sbjct: 421 PSDNIPTLRLGPCPKSDPWTFNPSEGILWI--NKMCVEAPNVVGQKVKLGVGTKCSKLGQ 480

Query: 481 QSAWRAISNSKLHLAVFTQDGKSLCLQIENSKSSKIVVSSCICTNGDSNCLQDTQSQWFT 533
                 IS +K+HL+  T +G  LCL ++  + + +V + C     D++C  D  SQWF 
Sbjct: 481 ------ISATKMHLSFKTSNGLLLCLDVD-ERDNSVVANRCKFLTMDASC--DPASQWFK 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA06.2e-11842.31Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P545836.9e-1622.95Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351... [more]
P194872.9e-1424.21Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
A0A5A7TMC95.6e-27184.11Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG18.1e-27083.93Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0K8531.2e-26883.64Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
A0A5A7TMX02.6e-26883.77Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1JZU82.4e-26682.87Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900453.17.2e-28989.57glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
KAA0042711.11.2e-27084.11major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042713.11.7e-26983.93major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAE8646606.12.4e-26883.64hypothetical protein Csa_005432 [Cucumis sativus][more]
XP_011658389.12.4e-26883.64glycosyl hydrolase 5 family protein [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G13130.13.6e-11340.27Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.11.1e-10640.15Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.13.6e-10538.72Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.2e-10036.16Glycosyl hydrolase superfamily protein [more]
AT5G16700.16.1e-8434.87Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 63..350
e-value: 1.0E-26
score: 94.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..393
e-value: 1.5E-68
score: 233.6
NoneNo IPR availableGENE3D2.80.10.50coord: 404..536
e-value: 3.9E-5
score: 25.8
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 7..535
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 400..531
score: 10.239
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 410..518
e-value: 0.00144937
score: 36.7147
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 402..517
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..380

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g002640.1Lcy07g002640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds