Lcy07g002340 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g002340
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationChr07: 9104111 .. 9105917 (+)
RNA-Seq ExpressionLcy07g002340
SyntenyLcy07g002340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATAACTTTACAAACTATGGTATTTTTAGCACTAGTCCTTGTGTTTTCATCTCTTTCAGCTTATTCCTTACCTTTGTCGACAAAAGGAAGATGGATCGTCGATTCAAAGACAGGGAATCGAGTCAAGCTTGTGTGCATGAATTGGCCTTCTCACACCCAAAGTATGTTGGCAGAAGGCCTCAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATTAAACTGAGGTTTAATTGTGTGCGTCTCACATACGCAACCCACATGTTCACTCGTTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATTTAAAAGAAGCCAAGGCTGGATTGGCCAAATATAACCCTTTTGTTTTAAACAAGACCATCGTTGAAGCCTACGAAGCTGTTGTGGATGTGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGTAACGGCTTCTTTGGTAATCGTAATTTCGACCCTCAAGAATGGTTACAAGGTCTACGCTTAGTTGCAGAACGTTTTGTTAACAAATCAGCGGTATGTAACATTAAAAAATATTCTCATGTTCTTGAAATTATCCCAACCATTTGAAGACTAGAAGCTAAATTATTTCATTAACGAGATTTTTATTTTGCATATAGTTGGTAGCAATGAGCCTACGGAATGAGATACGAGGAATGATGGAAAATGCGAATGATTGGAACAACTATATAACTCAAGGAGTAACAACAATCCACAACACAAATTCAGAAGTTTTAGTTATTGTGTCTGGTCTAAATTATGACAACGATCTTCGATGCTTGAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAACAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAAAGCAAATTTGTACAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTCATAGATCATGCTGAGTTTGTAATAGAAGGACCAAACCCATTTCCTCTATTTGTTAGTGAATATGGGTACGATCAAAGAGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCGGAAAGACTTGGATTGGGCACTATGGAGTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAGCAGAGCCTGGAGAAGCATTTGGCGTTCTCAACTCGGACTGGACTCAAATTAAAAACCCAAACTTTTCTAAGAAGTTTCAATTATTGCAGACCATGTTGCAAGGTAATTAAATTAATGATTTTCAATACATTCAACTATTAAGGTAGTTTGTATGATCAAAATATAATTCATATAACATTTTTATGACACAGATCCAAATTCAAATGCTTCATCCTCATATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCCTCGAATGACAATACAGGAATTTTTTTGAGCAATTGTTCCACCTCAAGTCATTGGAGTCATGACGGTGATGGTGCCCCAATTAGGATGACATCAAATGGTTTGTGTTTGAAGGCCAATGGAGAAGCTCTTGGGGCATCCCTGTCGAGTGACTGCTCAAGTCAACAGAGTGCTTGGAAAGCCATTTCTAACTCTAAGCTTCACCTGGCTACCTTCACTCAAGATGGGAAGAACCTTTGCTTACAAATTGAAAATTCTAATTCTACAAAAATTGTAGTAAACTCTTGTATTTGCACCAATGGTGACTCACATTGCCTCCAAGACACACAAAGTCAATGGTTCACACTTGTTCCAACCAATGCCTTGTAA

mRNA sequence

ATGGGAATAACTTTACAAACTATGGTATTTTTAGCACTAGTCCTTGTGTTTTCATCTCTTTCAGCTTATTCCTTACCTTTGTCGACAAAAGGAAGATGGATCGTCGATTCAAAGACAGGGAATCGAGTCAAGCTTGTGTGCATGAATTGGCCTTCTCACACCCAAAGTATGTTGGCAGAAGGCCTCAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATTAAACTGAGGTTTAATTGTGTGCGTCTCACATACGCAACCCACATGTTCACTCGTTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATTTAAAAGAAGCCAAGGCTGGATTGGCCAAATATAACCCTTTTGTTTTAAACAAGACCATCGTTGAAGCCTACGAAGCTGTTGTGGATGTGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGTAACGGCTTCTTTGGTAATCGTAATTTCGACCCTCAAGAATGGTTACAAGGTCTACGCTTAGTTGCAGAACGTTTTGTTAACAAATCAGCGTTGGTAGCAATGAGCCTACGGAATGAGATACGAGGAATGATGGAAAATGCGAATGATTGGAACAACTATATAACTCAAGGAGTAACAACAATCCACAACACAAATTCAGAAGTTTTAGTTATTGTGTCTGGTCTAAATTATGACAACGATCTTCGATGCTTGAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAACAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAAAGCAAATTTGTACAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTCATAGATCATGCTGAGTTTGTAATAGAAGGACCAAACCCATTTCCTCTATTTGTTAGTGAATATGGGTACGATCAAAGAGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCGGAAAGACTTGGATTGGGCACTATGGAGTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAGCAGAGCCTGGAGAAGCATTTGGCGTTCTCAACTCGGACTGGACTCAAATTAAAAACCCAAACTTTTCTAAGAAGTTTCAATTATTGCAGACCATGTTGCAAGATCCAAATTCAAATGCTTCATCCTCATATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCCTCGAATGACAATACAGGAATTTTTTTGAGCAATTGTTCCACCTCAAGTCATTGGAGTCATGACGGTGATGGTGCCCCAATTAGGATGACATCAAATGGTTTGTGTTTGAAGGCCAATGGAGAAGCTCTTGGGGCATCCCTGTCGAGTGACTGCTCAAGTCAACAGAGTGCTTGGAAAGCCATTTCTAACTCTAAGCTTCACCTGGCTACCTTCACTCAAGATGGGAAGAACCTTTGCTTACAAATTGAAAATTCTAATTCTACAAAAATTGTAGTAAACTCTTGTATTTGCACCAATGGTGACTCACATTGCCTCCAAGACACACAAAGTCAATGGTTCACACTTGTTCCAACCAATGCCTTGTAA

Coding sequence (CDS)

ATGGGAATAACTTTACAAACTATGGTATTTTTAGCACTAGTCCTTGTGTTTTCATCTCTTTCAGCTTATTCCTTACCTTTGTCGACAAAAGGAAGATGGATCGTCGATTCAAAGACAGGGAATCGAGTCAAGCTTGTGTGCATGAATTGGCCTTCTCACACCCAAAGTATGTTGGCAGAAGGCCTCAACCATAGACCACTGAAAGAACTTGCAGATGAGGCAATTAAACTGAGGTTTAATTGTGTGCGTCTCACATACGCAACCCACATGTTCACTCGTTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATTTAAAAGAAGCCAAGGCTGGATTGGCCAAATATAACCCTTTTGTTTTAAACAAGACCATCGTTGAAGCCTACGAAGCTGTTGTGGATGTGCTTGGGGCGAGTGGTTTAATGGTTATTGCCGACAACCACATTAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGTAACGGCTTCTTTGGTAATCGTAATTTCGACCCTCAAGAATGGTTACAAGGTCTACGCTTAGTTGCAGAACGTTTTGTTAACAAATCAGCGTTGGTAGCAATGAGCCTACGGAATGAGATACGAGGAATGATGGAAAATGCGAATGATTGGAACAACTATATAACTCAAGGAGTAACAACAATCCACAACACAAATTCAGAAGTTTTAGTTATTGTGTCTGGTCTAAATTATGACAACGATCTTCGATGCTTGAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAACAAGTTAGTTTTCGAAGTCCACTTATATTCCTTTAGTGGAGATTCTGAAAGCAAATTTGTACAACAACCATTGAACAATATTTGTGCAAATATCATGAATGGGTTCATAGATCATGCTGAGTTTGTAATAGAAGGACCAAACCCATTTCCTCTATTTGTTAGTGAATATGGGTACGATCAAAGAGAGGTTAATGATGCTGAAAATCGATTCATGAGTTGCTTTATGGCTCATCTTGCTCGGAAAGACTTGGATTGGGCACTATGGAGTTGGCAAGGTAGCTATTATTATAGGGAAGGTCAAGCAGAGCCTGGAGAAGCATTTGGCGTTCTCAACTCGGACTGGACTCAAATTAAAAACCCAAACTTTTCTAAGAAGTTTCAATTATTGCAGACCATGTTGCAAGATCCAAATTCAAATGCTTCATCCTCATATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCCTCGAATGACAATACAGGAATTTTTTTGAGCAATTGTTCCACCTCAAGTCATTGGAGTCATGACGGTGATGGTGCCCCAATTAGGATGACATCAAATGGTTTGTGTTTGAAGGCCAATGGAGAAGCTCTTGGGGCATCCCTGTCGAGTGACTGCTCAAGTCAACAGAGTGCTTGGAAAGCCATTTCTAACTCTAAGCTTCACCTGGCTACCTTCACTCAAGATGGGAAGAACCTTTGCTTACAAATTGAAAATTCTAATTCTACAAAAATTGTAGTAAACTCTTGTATTTGCACCAATGGTGACTCACATTGCCTCCAAGACACACAAAGTCAATGGTTCACACTTGTTCCAACCAATGCCTTGTAA

Protein sequence

MGITLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRLVAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPGEAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGIFLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLHLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL
Homology
BLAST of Lcy07g002340 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.2e-114
Identity = 224/539 (41.56%), Postives = 320/539 (59.37%), Query Frame = 0

Query: 12  ALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLNHRPLKELA 71
           AL+L+  +  ++SLPL T+GRWIVD  TG RVKL C+NW  H +  L EGLN  P+  +A
Sbjct: 16  ALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVA 75

Query: 72  DEAIKLRFNCVRLTYATHMFTR--YANRTIEENFDLLDLKEAKAGLAKYNPFVLNKTIVE 131
                L FNCVRLTY+ HM TR  Y N T+ + F  L+L EA +G+   NP +L+   V 
Sbjct: 76  HTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVA 135

Query: 132 AYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRLVAERFV 191
           AY  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+R F+P  W++GL L+A  F 
Sbjct: 136 AYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFN 195

Query: 192 NKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDNDLRCLKE 251
           N   +VAMSLRNE+RG       W+ ++  G  T+H  N +VLVI+SGL +D DL  L  
Sbjct: 196 NTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPV 255

Query: 252 KPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGPN--P 311
            P+ +     K+V+E H YSF     +       N++C N    F  +  FV    N   
Sbjct: 256 LPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSANATA 315

Query: 312 FPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQ---AEPGE 371
            PLF+SE+G DQR VND +NR+++C +A+LA +DLDWALW+  GSYYYR  +    +  E
Sbjct: 316 APLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEE 375

Query: 372 AFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSY-VMYHPQSGQCAQASNDNTGI 431
            +G  N DW++I+NP+F  + + +Q  +QDP       Y ++YHP SG C ++   NT +
Sbjct: 376 TYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNT-V 435

Query: 432 FLSNC-STSSHWSHDGD-GAPIRMTSNGLCLKANGEALGASLSSDCSS-QQSAWKAISNS 491
            L +C S  S W++D     PI +  +  C+   G  L A ++ +CS+   + W  +S++
Sbjct: 436 HLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSA 495

Query: 492 KLHLAT--FTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQ--DTQSQWFTLVPTN 536
           +L L T    +DGK   + ++ S S  I  N CIC   DSHC    + + QWF ++ TN
Sbjct: 496 QLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVITTN 547

BLAST of Lcy07g002340 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 1.8e-11
Identity = 96/405 (23.70%), Postives = 159/405 (39.26%), Query Frame = 0

Query: 11  LALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMN-WPSHTQSMLAEGLNHRPLKE 70
           LA  L  ++  A+S  ++   R IVD  +G  V+L  +N +   T + +  GL  R  K+
Sbjct: 12  LATALALAAGPAFSYSIN-NSRQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKD 71

Query: 71  LADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKEAKAGLAKYNPFVLNKT 130
           +  +   L FN VRL +        T  A+     N DL  L   +         +L+K 
Sbjct: 72  MIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ---------ILDKV 131

Query: 131 IVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRLVAE 190
           I E          A G+ V+ D+H      C  + +    +   ++   +WL  LR VA 
Sbjct: 132 IAE--------FNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLRFVAN 191

Query: 191 RFVNKSALVAMSLRNEIRGMM-----ENANDWNNYITQGVTTIHNTNSEVLVIVSGLN-- 250
           R+ N   ++ + L+NE  G         A DWN    +G   +     + L+ V G+   
Sbjct: 192 RYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDN 251

Query: 251 ----------YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAN 310
                     +  +L+ L   PLN+    N+L+   H+Y         FVQ   N+  +N
Sbjct: 252 PVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVY-----GPDVFVQSYFND--SN 311

Query: 311 IMNGF--IDHAEFVIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWAL- 370
             N    I    F  +      L + E+G    E +  +  +    + +L  K ++    
Sbjct: 312 FPNNMPAIWERHFG-QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFY 368

Query: 371 WSWQGSYYYREGQAEPGEAFGVLNSDWTQIKNPNFSKKFQLLQTM 392
           WSW             G+  G+L  DWT ++      K  LL+T+
Sbjct: 372 WSW---------NPNSGDTGGILRDDWTSVR----QDKMTLLRTL 368

BLAST of Lcy07g002340 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.6e-268
Identity = 447/534 (83.71%), Postives = 486/534 (91.01%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T  T++ +ALV VFS LS  SLPLST+GRWI+DS+TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 3   TFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLN 62

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA+YNPFVL
Sbjct: 63  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVL 122

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 123 NKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 182

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 183 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 242

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++
Sbjct: 243 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQ 302

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 303 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 362

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSN S SYV+YHPQS QC Q SNDN  I
Sbjct: 363 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKEI 422

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FL++CST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC SQQS W AISNSKLH
Sbjct: 423 FLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLH 482

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           LAT TQ GK+LCLQI++SNS+K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 483 LATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of Lcy07g002340 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.4e-267
Identity = 444/534 (83.15%), Postives = 483/534 (90.45%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T  T++ LALV VFS LS  SLPLST+GRWI+D +TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 3   TFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGLN 62

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA+YNPFVL
Sbjct: 63  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVL 122

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 123 NKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 182

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 183 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 242

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV++
Sbjct: 243 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQ 302

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 303 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 362

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSNAS SYV+YHPQ+ QC Q SNDN  I
Sbjct: 363 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKEI 422

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FL++CST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC  QQS W AISNSKLH
Sbjct: 423 FLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKLH 482

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           LAT  Q G +LCLQI++SNS+K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 483 LATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of Lcy07g002340 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.4e-266
Identity = 446/535 (83.36%), Postives = 484/535 (90.47%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T   ++ LALV VFS L A SLPLST+GRWI+DS+TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 6   TFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLN 65

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA YNPFVL
Sbjct: 66  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFVL 125

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 126 NKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 185

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 186 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 245

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++
Sbjct: 246 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQ 305

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 306 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 365

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSNAS SYV+YHPQS QC Q SNDN  I
Sbjct: 366 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNKEI 425

Query: 424 FLSNC-STSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKL 483
           FL++C ST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC SQQS W AISNSKL
Sbjct: 426 FLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 485

Query: 484 HLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           HLAT TQ GK+LCLQI++SN +K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 486 HLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of Lcy07g002340 vs. ExPASy TrEMBL
Match: A0A6J1H6A6 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459997 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 8.7e-264
Identity = 437/532 (82.14%), Postives = 480/532 (90.23%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           TLQ +V LALV +FSSLS  SLPLST+GRWI+DSKTG RVKLVC+NWPSHTQSML EGLN
Sbjct: 4   TLQAIVCLALVFIFSSLSTCSLPLSTRGRWIIDSKTGRRVKLVCVNWPSHTQSMLIEGLN 63

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLKELADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLK AKAGL +YNPFVL
Sbjct: 64  HRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKPAKAGLVQYNPFVL 123

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EA+EAVVDVLG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQGL+L
Sbjct: 124 NKTIAEAFEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQGLQL 183

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA RF+NK A++AMSLRNEIRG  ENANDWNNYITQGVT +HNTN ++LVIVSGLN+DND
Sbjct: 184 VAWRFINKPAMIAMSLRNEIRGTRENANDWNNYITQGVTIVHNTNPDILVIVSGLNFDND 243

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNV  LDNKLVFEVHLYS SGD ESKFVQQPLNNICANI+NGF+DHA FV++
Sbjct: 244 LRCLKEKPLNVTNLDNKLVFEVHLYSLSGDPESKFVQQPLNNICANIINGFVDHAGFVMD 303

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           GPNPFPLFVSEYGYDQRE NDAENRFMSCF +HLA+KDLDWALW+WQGSYYYREGQAEP 
Sbjct: 304 GPNPFPLFVSEYGYDQRESNDAENRFMSCFTSHLAQKDLDWALWTWQGSYYYREGQAEPT 363

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E FGVLNS+WT+I+NPNFSKKF LLQ+MLQDPNSNAS SY++YHPQSGQCAQ SN++T I
Sbjct: 364 EVFGVLNSNWTKIQNPNFSKKFHLLQSMLQDPNSNASFSYILYHPQSGQCAQTSNNDTQI 423

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FLS+CSTSS WSH   G  I+M + GLCLKANGE LG SLSSDC SQQS W+ ISN+ LH
Sbjct: 424 FLSDCSTSSRWSHGDGGNSIKMATTGLCLKANGEGLGVSLSSDCLSQQSVWRTISNTNLH 483

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTN 536
           LAT TQDGK LCLQ+E+S+S+KIV N CICTNG+ +CLQDTQSQWF LV TN
Sbjct: 484 LATVTQDGKYLCLQVESSSSSKIVTNPCICTNGEPNCLQDTQSQWFQLVATN 535

BLAST of Lcy07g002340 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.3e-263
Identity = 443/535 (82.80%), Postives = 479/535 (89.53%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T+Q ++ LALV VFSS SAYSLPLST GRWI+DS++G RVKLVC+NWPSHTQSML EGLN
Sbjct: 4   TIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLN 63

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL++AKAGLA+YNPFVL
Sbjct: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVL 123

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGNR FDPQEWLQGL L
Sbjct: 124 NKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSL 183

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNE+RGMMENANDWNNY+TQGVTTIH  N  VLVIVSGLNYDND
Sbjct: 184 VAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDND 243

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEFVIE
Sbjct: 244 LRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIE 303

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           GPNPFPLFVSEYGYDQREV+DAENRFMSCF AHLA+KDLDWALW+WQGSYYYREGQAE  
Sbjct: 304 GPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELA 363

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E FGVL+S+WTQIKNPNF +KFQLLQTMLQDP SNAS SYV+YH QSGQC + SNDN  I
Sbjct: 364 ETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKEI 423

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FL+NCSTSS WSHD D  PI+M+S GLCLKA+GE L ASLS+DC  +QS W AISNS LH
Sbjct: 424 FLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNLH 483

Query: 484 LATFTQDGKNLCLQ-IENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           L T T+DGK+LCLQ IE+SNS+KIV NSCICT  D  CLQDTQSQWF LV TN L
Sbjct: 484 LGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of Lcy07g002340 vs. NCBI nr
Match: XP_038900453.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 979.2 bits (2530), Expect = 1.5e-281
Identity = 472/537 (87.90%), Postives = 499/537 (92.92%), Query Frame = 0

Query: 1   MGITLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAE 60
           MG  LQT+VFL LV  F   SAYSLPLSTKGRWIVDSKTG+RVK VC NWPSHTQSMLAE
Sbjct: 1   MGRNLQTIVFLVLVFEFLFFSAYSLPLSTKGRWIVDSKTGHRVKFVCANWPSHTQSMLAE 60

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNP 120
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA++NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQHNP 120

Query: 121 FVLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
           F+LNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG
Sbjct: 121 FLLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180

Query: 181 LRLVAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNY 240
           LRLVA+RFVNKS +VAMSLRNEIRG MENANDWNNY+TQGVTTIHN N EVLVIVSGLNY
Sbjct: 181 LRLVAKRFVNKSTVVAMSLRNEIRGTMENANDWNNYVTQGVTTIHNINPEVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 300
           DNDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV QPLN+ICA IMNGF+DHA F
Sbjct: 241 DNDLRCLKEKPLNVNTLDNKLVFEVHLYSFSGDSESKFVTQPLNDICATIMNGFVDHAGF 300

Query: 301 VIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQREVN+AENRFMSCF AHLA+KDLDWALW+WQGSYYYREGQA
Sbjct: 301 VIEGSNPFPLFVSEYGYDQREVNEAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPGEAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDN 420
           EP E FGVL+S+WTQIKNPNF KKFQLLQTMLQDPNSNAS+SYVMYHPQSGQC QA +DN
Sbjct: 361 EPAETFGVLDSNWTQIKNPNFPKKFQLLQTMLQDPNSNASTSYVMYHPQSGQCVQALDDN 420

Query: 421 TGIFLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNS 480
            G+FL+NCST S WSH+GDG+PIR+ S GLCLKANGE LGASLSSDC SQQSAW+AISNS
Sbjct: 421 KGMFLNNCSTPSRWSHNGDGSPIRLRSTGLCLKANGEGLGASLSSDCLSQQSAWRAISNS 480

Query: 481 KLHLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           KLHLATF QDGK+LCLQ+ENSNS+KIVVNSCICTNGD  CLQDTQSQW TLV TN L
Sbjct: 481 KLHLATFAQDGKSLCLQLENSNSSKIVVNSCICTNGDPKCLQDTQSQWLTLVATNTL 537

BLAST of Lcy07g002340 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 934.1 bits (2413), Expect = 5.4e-268
Identity = 447/534 (83.71%), Postives = 486/534 (91.01%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T  T++ +ALV VFS LS  SLPLST+GRWI+DS+TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 3   TFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLN 62

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA+YNPFVL
Sbjct: 63  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVL 122

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 123 NKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 182

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 183 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 242

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++
Sbjct: 243 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQ 302

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 303 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 362

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSN S SYV+YHPQS QC Q SNDN  I
Sbjct: 363 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKEI 422

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FL++CST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC SQQS W AISNSKLH
Sbjct: 423 FLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLH 482

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           LAT TQ GK+LCLQI++SNS+K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 483 LATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of Lcy07g002340 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 929.1 bits (2400), Expect = 1.7e-266
Identity = 444/534 (83.15%), Postives = 483/534 (90.45%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T  T++ LALV VFS LS  SLPLST+GRWI+D +TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 3   TFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGLN 62

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA+YNPFVL
Sbjct: 63  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVL 122

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 123 NKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 182

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 183 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 242

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV++
Sbjct: 243 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQ 302

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 303 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 362

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSNAS SYV+YHPQ+ QC Q SNDN  I
Sbjct: 363 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKEI 422

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FL++CST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC  QQS W AISNSKLH
Sbjct: 423 FLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKLH 482

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           LAT  Q G +LCLQI++SNS+K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 483 LATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of Lcy07g002340 vs. NCBI nr
Match: KAA0042709.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 926.4 bits (2393), Expect = 1.1e-265
Identity = 446/535 (83.36%), Postives = 484/535 (90.47%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           T   ++ LALV VFS L A SLPLST+GRWI+DS+TG RVKLVCMNWPSHTQSML EGLN
Sbjct: 6   TFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLN 65

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLK+AKAGLA YNPFVL
Sbjct: 66  HRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFVL 125

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGL L
Sbjct: 126 NKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSL 185

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA+RF NKS +V MSLRNEIRG MENANDWN YITQGVTTIHN NSEVLVIVSGLNYDND
Sbjct: 186 VAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDND 245

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++
Sbjct: 246 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQ 305

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           G NPFPLFVSE+GYDQREVNDAENRFMSCF AHLA +DLDWALW+WQGSYY+REGQAEPG
Sbjct: 306 GSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPG 365

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E+FGVL+S+WTQ+KNPNF+KKFQLLQTMLQDPNSNAS SYV+YHPQS QC Q SNDN  I
Sbjct: 366 ESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNKEI 425

Query: 424 FLSNC-STSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKL 483
           FL++C ST + WSH  D  PI+M+S GLCLKA+GE L ASLS+DC SQQS W AISNSKL
Sbjct: 426 FLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 485

Query: 484 HLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTNAL 538
           HLAT TQ GK+LCLQI++SN +K+V NSCICTNGD +CLQDT+SQWF LV TN L
Sbjct: 486 HLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of Lcy07g002340 vs. NCBI nr
Match: XP_022958844.1 (uncharacterized protein LOC111459997 [Cucurbita moschata])

HSP 1 Score: 919.1 bits (2374), Expect = 1.8e-263
Identity = 437/532 (82.14%), Postives = 480/532 (90.23%), Query Frame = 0

Query: 4   TLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 63
           TLQ +V LALV +FSSLS  SLPLST+GRWI+DSKTG RVKLVC+NWPSHTQSML EGLN
Sbjct: 4   TLQAIVCLALVFIFSSLSTCSLPLSTRGRWIIDSKTGRRVKLVCVNWPSHTQSMLIEGLN 63

Query: 64  HRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKEAKAGLAKYNPFVL 123
           HRPLKELADEAIKL+FNCVRLTYATHMFTRYANRTIEENFDLLDLK AKAGL +YNPFVL
Sbjct: 64  HRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKPAKAGLVQYNPFVL 123

Query: 124 NKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRL 183
           NKTI EA+EAVVDVLG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQGL+L
Sbjct: 124 NKTIAEAFEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQGLQL 183

Query: 184 VAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDND 243
           VA RF+NK A++AMSLRNEIRG  ENANDWNNYITQGVT +HNTN ++LVIVSGLN+DND
Sbjct: 184 VAWRFINKPAMIAMSLRNEIRGTRENANDWNNYITQGVTIVHNTNPDILVIVSGLNFDND 243

Query: 244 LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 303
           LRCLKEKPLNV  LDNKLVFEVHLYS SGD ESKFVQQPLNNICANI+NGF+DHA FV++
Sbjct: 244 LRCLKEKPLNVTNLDNKLVFEVHLYSLSGDPESKFVQQPLNNICANIINGFVDHAGFVMD 303

Query: 304 GPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPG 363
           GPNPFPLFVSEYGYDQRE NDAENRFMSCF +HLA+KDLDWALW+WQGSYYYREGQAEP 
Sbjct: 304 GPNPFPLFVSEYGYDQRESNDAENRFMSCFTSHLAQKDLDWALWTWQGSYYYREGQAEPT 363

Query: 364 EAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGI 423
           E FGVLNS+WT+I+NPNFSKKF LLQ+MLQDPNSNAS SY++YHPQSGQCAQ SN++T I
Sbjct: 364 EVFGVLNSNWTKIQNPNFSKKFHLLQSMLQDPNSNASFSYILYHPQSGQCAQTSNNDTQI 423

Query: 424 FLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAISNSKLH 483
           FLS+CSTSS WSH   G  I+M + GLCLKANGE LG SLSSDC SQQS W+ ISN+ LH
Sbjct: 424 FLSDCSTSSRWSHGDGGNSIKMATTGLCLKANGEGLGVSLSSDCLSQQSVWRTISNTNLH 483

Query: 484 LATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLVPTN 536
           LAT TQDGK LCLQ+E+S+S+KIV N CICTNG+ +CLQDTQSQWF LV TN
Sbjct: 484 LATVTQDGKYLCLQVESSSSSKIVTNPCICTNGEPNCLQDTQSQWFQLVATN 535

BLAST of Lcy07g002340 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.8e-113
Identity = 207/514 (40.27%), Postives = 318/514 (61.87%), Query Frame = 0

Query: 24  SLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLNHRPLKELADEAIKLRFNCVR 83
           S PLST  RWIVD + G RVKLVC NWPSH Q ++AEGL+ +P+  +A + +++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 84  LTYATHMFTRYA---NRTIEENFDLLDLKEAKAGLAKYNPFVLNKTIVEAYEAVVDVLGA 143
           LT+   + T      N T+ ++F  L L +   G    NP +++  ++EAY+ VV  LG 
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 144 SGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRLVAERFVNKSALVAMSLR 203
           + +MVI DNH+++P WCC+ DDGNGFFG++ FDP  W+  L+ +A  F   S +V MSLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 204 NEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNK 263
           NE+RG  +N NDW  Y+ QG   +H+ N++VLVI+SGL++D DL  ++ +P+ + +   K
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKL-SFTGK 271

Query: 264 LVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGPNPFPLFVSEYGYDQR 323
           LVFE+H YSFS D  S     P N+IC  ++N   +   +++     FPLF+SE+G D+R
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLLN--QGFPLFLSEFGIDER 331

Query: 324 EVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPGEAFGVLNSDWTQIKNPN 383
            VN  +NR+  C     A  D+DW+LW+  GSYY R+G+    E +GVL+SDW  ++N +
Sbjct: 332 GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSS 391

Query: 384 FSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASNDNTGIF-LSNCSTSSHWSHDGD 443
           F +K   LQ+ LQ P     +  +++HP +G C   S D+  +  L  C++S  WS+   
Sbjct: 392 FLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKK 451

Query: 444 GAPIRMTSNGLCLKANGEALGASLS-SDCSSQQSAWKAISNSKLHLATFTQDGKNLCLQI 503
              +R+    LCL++NG     +++ + CS+  S W+ IS S++HLA+ T +  +LCL +
Sbjct: 452 A--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDV 511

Query: 504 ENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLV 533
           + +N+  +V N+C C + D  C  +  SQWF ++
Sbjct: 512 DTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of Lcy07g002340 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 374.4 bits (960), Expect = 1.5e-103
Identity = 210/534 (39.33%), Postives = 308/534 (57.68%), Query Frame = 0

Query: 5   LQTMVFLALVLVFSSLSAYSLPLSTKGRWIV-DSKTGNRVKLVCMNWPSHTQSMLAEGLN 64
           ++   F+++ L+   ++ ++ P ST  RWIV D   G RVKL C+NWPSH ++ +AEGL+
Sbjct: 1   MEKFFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLS 60

Query: 65  HRPLKELADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKEAKAGLAKYNP 124
            +PL  +A++ + + FNCVRLT+  ++ T     A  T+ ++     L EA +G   +NP
Sbjct: 61  KQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNP 120

Query: 125 FVLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 184
            +L+  +++A++ VV  L    +MVI DNHISQP WCCS +DGNGFFG+++ +PQ W++G
Sbjct: 121 TILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKG 180

Query: 185 LRLVAERFVNKSA-LVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLN 244
           L+ +A  F N S+ +V MSLRNE+RG  +N  DW  Y+ +G   +H+ N  VLVIVSGLN
Sbjct: 181 LKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLN 240

Query: 245 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAE 304
           Y  DL  L+E+P  V +   K+VFE+H Y F    E       LN IC       +  + 
Sbjct: 241 YATDLSFLRERPFEV-SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSG 300

Query: 305 FVIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQ 364
           F++E     PLFVSE+G DQR  N  +N+F+SCFMA  A +DLDW+LW+  GSYY RE  
Sbjct: 301 FLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKS 360

Query: 365 AEPGEAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCAQASND 424
               E++GVL+ +W+ I+N    +    +QT             +M+HP +G C      
Sbjct: 361 IGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIG-LMETQPKKIMFHPSTGLCI-VRKS 420

Query: 425 NTGIFLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCS-SQQSAWKAIS 484
              + L +C+ S  W               LCLKA  +     L    S S  S WK  S
Sbjct: 421 LFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFS 480

Query: 485 NSKLHLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLV 533
           +SK+ L++ T++G ++CL ++  N+  IV NSC C  G+S C  D +SQWF LV
Sbjct: 481 DSKMQLSSITKNGFSVCLDVDTENN-NIVTNSCKCLRGNSSC--DPRSQWFKLV 522

BLAST of Lcy07g002340 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.2e-102
Identity = 198/514 (38.52%), Postives = 303/514 (58.95%), Query Frame = 0

Query: 26  PLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLNHRPLKELADEAIKLRFNCVRLT 85
           PLST  RWI+D K G RVKL C+NWPSH Q ++AEGL+ + + +LA + + + FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRYA---NRTIEENFDLLDLKEAKAGLAKYNPFVLNKTIVEAYEAVVDVLGASG 145
           +   + T      N T+ ++F  L L +  +G    NP +++  ++EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLRLVAERFVNKSALVAMSLRNE 205
           +MVI DNH+++P WCC  +DGNGFFG+  FDP  W+ GL  +A  F   + +V MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 IRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLV 265
           +RG  +N +DW  Y+ QG   +H  N  VLVI+SGL+YD DL  ++ + +N+ T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 266 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGPNPFPLFVSEYGYDQREV 325
           FE+H YSF+  + + +  +  N  C  I+    +   F +     FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGK 303

Query: 326 NDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQAEPGEAFGVLNSDWTQIKNPNFS 385
           N  +NR++ C +   A  D+DW++W+ QGSYY REG     E +G+L+SDW ++++ +F 
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 386 KKFQLLQTMLQDPNSNASSSYVMYHPQSGQC-AQASNDNTGIFLSNCSTSSHWSHDGDGA 445
           ++  L+ + LQ P S +    +++HP +G C  Q+  D T + L  C+ S  WS+     
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 446 PIRMTSNGLCLKANGEALGASLS-SDCSSQQ-SAWKAISNSKLHLATFTQDGKNLCLQIE 505
            + +    LCL++ G      LS + CSS   S W+ IS S + LA       +LCL ++
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAA-KSTNNSLCLDVD 483

Query: 506 NSNSTKIVVNSCICTNG-DSHCLQDTQSQWFTLV 533
            +N+  ++ ++C C  G DS C  D  SQWF +V
Sbjct: 484 ETNN--LMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of Lcy07g002340 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 351.7 bits (901), Expect = 1.1e-96
Identity = 197/536 (36.75%), Postives = 311/536 (58.02%), Query Frame = 0

Query: 3   ITLQTMVFLALVLVFSSLSAYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGL 62
           I L   +FL L L+  +L A   PL TK RWIV++K G+RVKL C NWPSH + ++AEGL
Sbjct: 5   IALTVFLFLFLSLISLTL-ATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGL 64

Query: 63  NHRPLKELADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKEAKAGLAKYN 122
           + +P+  ++ +   + FNCVRLT+   +    T   N T++++F+   L     G+  +N
Sbjct: 65  SSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHN 124

Query: 123 PFVLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQ 182
           P+++N  ++  ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+ 
Sbjct: 125 PYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWML 184

Query: 183 GLRLVAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLN 242
           GL+ +A  F+N   +V MSLRNE+RG    + DW  Y+ +G   +H +N  VLVI+SGLN
Sbjct: 185 GLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLN 244

Query: 243 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAE 302
           +D DL  LK++P+N+ +   KLV E+H YSF+ D   ++    +N+ C+ + +       
Sbjct: 245 FDADLSFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGG 304

Query: 303 FVIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQ 362
           FV++    FPLF+SE+G DQR  +   NR+M+C +A  A KDLDWA+W+  G YY+REG+
Sbjct: 305 FVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGK 364

Query: 363 AEPGEAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQC--AQAS 422
               EA+G+L+++W  + N  + ++  ++Q     P    +    ++HP +G C   ++ 
Sbjct: 365 RGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSH 424

Query: 423 NDNTGIFLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGAS--LSSDCSSQQSAWK 482
              + + L  C+    WS+   G       +  CL+    A+G S  L   C+  +    
Sbjct: 425 CHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGE-TAVGKSVKLGRICTKIEQ--- 484

Query: 483 AISNSKLHLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTL 532
            IS +K+HL+  T DG  +CL +++ N+  +V NSC C  GD+ C  +  SQWF +
Sbjct: 485 -ISATKMHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Lcy07g002340 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 8.2e-81
Identity = 185/534 (34.64%), Postives = 287/534 (53.75%), Query Frame = 0

Query: 6   QTMVFLALVLVFSSLS--AYSLPLSTKGRWIVDSKTGNRVKLVCMNWPSHTQSMLAEGLN 65
           ++  F    L  SS S    S PLSTK RWIVD K G RVKL C+NWP+H Q  +AEGL+
Sbjct: 3   RSFYFCLFFLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAEGLS 62

Query: 66  HRPLKELADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKEAKAGLAKYNP 125
            +PL  ++ + + + FNCVRLT+   + T        T++++F+ L L E   G+  +NP
Sbjct: 63  KQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNP 122

Query: 126 FVLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 185
            +L+  +  A++ VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +G
Sbjct: 123 KLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKG 182

Query: 186 LRLVAERFVNKSALVAMSLRNEIRGMMENANDWNNYITQGVTTIHNTNSEVLVIVSGLNY 245
           LR +A  F N + ++ MSLRNE RG  +  + W  ++ QG   +H  N ++LVI+SG+++
Sbjct: 183 LRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDF 242

Query: 246 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEF 305
           D +L  L+++ +NV   D KLVFE+H YSFS D    + +   N+ C  I+     +  F
Sbjct: 243 DTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGF 302

Query: 306 VIEGPNPFPLFVSEYGYDQREVNDAENRFMSCFMAHLARKDLDWALWSWQGSYYYREGQA 365
           ++     FPL +SE+G DQR  + + NR+M+C +A  A  DLDWA+W+  G YY R G  
Sbjct: 303 LL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG-- 362

Query: 366 EPGEAFGVLNSDWTQIKNPNFSKKFQLLQTMLQDPNSNASSSYVMYHPQSGQCA--QASN 425
            PG                               PN N     +++HP +G C     S+
Sbjct: 363 -PG-----------------------------LRPNKN-----LLFHPSTGLCVTNNPSD 422

Query: 426 DNTGIFLSNCSTSSHWSHDGDGAPIRMTSNGLCLKANGEALGASLSSDCSSQQSAWKAIS 485
           +   + L  C  S  W+ +     + +  N +C++A    +G  +     ++ S    IS
Sbjct: 423 NIPTLRLGPCPKSDPWTFNPSEGILWI--NKMCVEA-PNVVGQKVKLGVGTKCSKLGQIS 482

Query: 486 NSKLHLATFTQDGKNLCLQIENSNSTKIVVNSCICTNGDSHCLQDTQSQWFTLV 533
            +K+HL+  T +G  LCL ++  +++ +V N C     D+ C  D  SQWF ++
Sbjct: 483 ATKMHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA03.2e-11441.56Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P194871.8e-1123.70Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
A0A5A7TMC92.6e-26883.71Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG18.4e-26783.15Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMX05.4e-26683.36Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1H6A68.7e-26482.14Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459997 P... [more]
A0A0A0K8534.3e-26382.80Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
Match NameE-valueIdentityDescription
XP_038900453.11.5e-28187.90glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
KAA0042711.15.4e-26883.71major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042713.11.7e-26683.15major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042709.11.1e-26583.36major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
XP_022958844.11.8e-26382.14uncharacterized protein LOC111459997 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G13130.12.8e-11340.27Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.11.5e-10339.33Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.12.2e-10238.52Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.1e-9636.75Glycosyl hydrolase superfamily protein [more]
AT5G16700.18.2e-8134.64Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 63..350
e-value: 1.8E-25
score: 89.9
NoneNo IPR availableGENE3D2.80.10.50coord: 404..531
e-value: 7.1E-6
score: 28.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..396
e-value: 3.0E-65
score: 222.7
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 7..533
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 400..531
score: 10.1625
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 410..519
e-value: 1.6773E-5
score: 42.4927
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 399..511
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g002340.1Lcy07g002340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds