Lcy06g016870 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy06g016870
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Retrotransposon protein
Location	Chr06: 38741759 .. 38742534 (-)
RNA-Seq Expression	Lcy06g016870
Synteny	Lcy06g016870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTAATTCAATGTGGAAACAAAATTGTTTAGGTGCATTGGACGGTACGTACATAAAGGTTCATGTTAGTGCGGCTAATCGACCAAGGTATAGGACGCGTAAGGGTGAGATTACAACAAATGTATTGGGCGTTGTGTCGTCAAAAGGTGAATTTATTTTTGTTATGCCGAGATGGGAAGGTTCGGCTGCTGATTCTCGTCTACTCAGGGATGCAATATCACGCCCCAACGGACTAATAGTGTTGAAGGGTATGGAACTCAGTATCAATCTAAAATAGGAACACTTATGTCATCCTTAGGTATTAACCTTCTTTTCTGAATAGGCTACTACTACCTTTGTGATGTTGGATACCCAAACGCAGAGGGTTTCTTGGCACCTTACAGAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCGAGAGAGCATTCGATACTCTAAGAGGACGGTGGGTGATACTTCGGGGGAAGTCCTACTACCCTGTTCGGACTCAGTGTCGAATCATAACAGCGTGTTGTTTACTCCACAACCTTATCACCCGGGAGATGGGTCTAGATGTTGGGTTGGATGAAGGTGATATTGGTCGGTCTGAACCCATACCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCGAACAGGATGTTCAACGCGTGGGGGCAACATCACTGA

mRNA sequence

ATGTGTAATTCAATGTGGAAACAAAATTGTTTAGGTGCATTGGACGGTACGTACATAAAGGTTCATGTTAGTGCGGCTAATCGACCAAGGTATAGGACGCGTAAGGGTGAGATTACAACAAATGTATTGGGCGTTGTGTCGTCAAAAGGTGAATTTATTTTTGTTATGCCGAGATGGGAAGGTTCGGCTGCTGATTCTCGTCTACTCAGGGATGCAATATCACGCCCCAACGGACTAATAGTGTTGAAGGGCTACTACTACCTTTGTGATGTTGGATACCCAAACGCAGAGGGTTTCTTGGCACCTTACAGAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCGAGAGAGCATTCGATACTCTAAGAGGACGGTGGGTGATACTTCGGGGGAAGTCCTACTACCCTGTTCGGACTCAGTGTCGAATCATAACAGCGTGTTGTTTACTCCACAACCTTATCACCCGGGAGATGGGTCTAGATGTTGGGTTGGATGAAGGTGATATTGGTCGGTCTGAACCCATACCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCGAACAGGATGTTCAACGCGTGGGGGCAACATCACTGA

Coding sequence (CDS)

ATGTGTAATTCAATGTGGAAACAAAATTGTTTAGGTGCATTGGACGGTACGTACATAAAGGTTCATGTTAGTGCGGCTAATCGACCAAGGTATAGGACGCGTAAGGGTGAGATTACAACAAATGTATTGGGCGTTGTGTCGTCAAAAGGTGAATTTATTTTTGTTATGCCGAGATGGGAAGGTTCGGCTGCTGATTCTCGTCTACTCAGGGATGCAATATCACGCCCCAACGGACTAATAGTGTTGAAGGGCTACTACTACCTTTGTGATGTTGGATACCCAAACGCAGAGGGTTTCTTGGCACCTTACAGAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCGAGAGAGCATTCGATACTCTAAGAGGACGGTGGGTGATACTTCGGGGGAAGTCCTACTACCCTGTTCGGACTCAGTGTCGAATCATAACAGCGTGTTGTTTACTCCACAACCTTATCACCCGGGAGATGGGTCTAGATGTTGGGTTGGATGAAGGTGATATTGGTCGGTCTGAACCCATACCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCGAACAGGATGTTCAACGCGTGGGGGCAACATCACTGA

Protein sequence

MCNSMWKQNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDVGLDEGDIGRSEPIPLDGENITFIQSSTEWTQKRDDLANRMFNAWGQHH

Homology

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BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-12
Identity = 57/192 (29.69%), Postives = 83/192 (43.23%), Query Frame = 0


Query: 9   NCLGALDGTYIKVHVSAANRPRYRTRKGE--ITTNVLGVVSSKGEFIFVMPRWEGSAADS 68
           NC GA+D T+I +++ A          GE   +  +  VV     F+ V+  W GS  D 
Sbjct: 182 NCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLND- 241

Query: 69  RLLRDAISRPNGL--IVLKG-----------------YYYLCDVGYPNAEGFLAPYRGER 128
               D + + +G   +V KG                  Y + D G+P     L PY+G+ 
Sbjct: 242 ----DVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGK- 301

Query: 129 YHLTEWRGGGNAPTT-PREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQC-R 178
                       PT+ P+  FN +HS A    + A   L+ RW I+ G  + P R +  R
Sbjct: 302 ------------PTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPR 355

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BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.9e-12
Identity = 55/180 (30.56%), Postives = 82/180 (45.56%), Query Frame = 0


Query: 11  LGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLL- 70
           +G +D  ++ +    A    Y  RKG  + N L V   +G  + V   W GS  D  +L 
Sbjct: 145 MGVVDCIHVAIKAPNAEDLSYVNRKGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQ 204

Query: 71  RDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREF-FN 130
           + ++S      + K  + L D  +     FL  +     H+         P TP E+ +N
Sbjct: 205 QSSLSSQFEAGMHKDSWLLGDSSF-----FLRTWLMTPLHI---------PETPAEYRYN 264

Query: 131 MKHSSARNVIERAFDTLRGRWVIL---RGKSYYPVRTQCRIITACCLLHNLITREMGLDV 186
           M HS+  +VIE+ F TL  R+  L   +G   Y       II ACC+LHN I+ E G+DV
Sbjct: 265 MAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

[Close]

BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-11
Identity = 55/180 (30.56%), Postives = 82/180 (45.56%), Query Frame = 0


Query: 11  LGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLL- 70
           +G +D  ++ +    A    Y  RKG  + N L V   +G  + V   W GS  D  +L 
Sbjct: 145 IGVVDCMHVAIKAPNAEDLSYVNRKGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQ 204

Query: 71  RDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREF-FN 130
           + ++S      + K  + L D  +     FL  +     H+         P TP E+ +N
Sbjct: 205 QSSLSSQFEAGMHKESWLLGDSSF-----FLRTWLMTPLHI---------PETPAEYRYN 264

Query: 131 MKHSSARNVIERAFDTLRGRWVIL---RGKSYYPVRTQCRIITACCLLHNLITREMGLDV 186
           M HS+  +VIE+ F TL  R+  L   +G   Y       II ACC+LHN I+ E G+DV
Sbjct: 265 MAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

[Close]

BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.2e-10
Identity = 53/180 (29.44%), Postives = 79/180 (43.89%), Query Frame = 0


Query: 11  LGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLL- 70
           +GA+D  ++ +    A    Y  RKG  + N L V   +G  + V   W GS  D  +L 
Sbjct: 145 IGAVDCIHVAIKAPNAEDLSYVNRKGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQ 204

Query: 71  RDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREF-FN 130
           + ++S      + K  + L D  +      L P               + P TP E+ +N
Sbjct: 205 QSSLSSQFETGMPKDSWLLGDSSFFLHTWLLTPL--------------HIPETPAEYRYN 264

Query: 131 MKHSSARNVIERAFDTLRGRWVIL---RGKSYYPVRTQCRIITACCLLHNLITREMGLDV 186
             HS+  +VIE+   TL  R+  L   +G   Y       II ACC+LHN I+ E G+DV
Sbjct: 265 RAHSATHSVIEKTLRTLCCRFRCLDGSKGALQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

[Close]

BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot
Match: Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 51/180 (28.33%), Postives = 75/180 (41.67%), Query Frame = 0


Query: 11  LGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLL- 70
           +G  D  ++ +    A    Y  RKG  + N L V   +G  + V   W GS  D  +L 
Sbjct: 145 IGVADCIHVAIKAPNAEDLSYVNRKGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQ 204

Query: 71  RDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREF-FN 130
           R +++      + K  + L D  +      L P                 P T  E+ +N
Sbjct: 205 RSSLTSQFETGMPKDSWLLGDSSFFLRSWLLTPL--------------PIPETAAEYRYN 264

Query: 131 MKHSSARNVIERAFDTLRGRWVIL---RGKSYYPVRTQCRIITACCLLHNLITREMGLDV 186
             HS+  +VIER   TL  R+  L   +G   Y       II ACC+LHN I+ + G+DV
Sbjct: 265 RAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEKCSHIILACCVLHN-ISLDHGMDV 309

[Close]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL
Match: A0A1S3C1U8 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.8e-86
Identity = 157/224 (70.09%), Postives = 175/224 (78.12%), Query Frame = 0


Query: 6   WKQNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAAD 65
           W +NCLGALDGTYIKV+VSA +RPRYRTRKGE+ TNVLG   +KG+F+FV+  WEGSAAD
Sbjct: 94  WFENCLGALDGTYIKVNVSATDRPRYRTRKGEVATNVLGACDTKGDFVFVLFGWEGSAAD 153

Query: 66  SRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPRE 125
           SR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRGERYHL+EWRG  NAPTT RE
Sbjct: 154 SRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFLAPYRGERYHLSEWRGESNAPTTARE 213

Query: 126 FFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDV 185
           FFNMKHSSARNVIERAF  L+GRW ILRGKSYYPV  QCR I ACCLLHNLI REM    
Sbjct: 214 FFNMKHSSARNVIERAFGLLKGRWAILRGKSYYPVDVQCRTIMACCLLHNLINREM---- 273

Query: 186 GLDEGDIGRSEPIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
                    SE I      I +I++S EW++ RD LA+ MF+ W
Sbjct: 274 -------TNSEII-----EINYIEASNEWSEWRDQLAHTMFSDW 301

[Close]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL
Match: A0A5A7VG45 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1023G00220 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 4.1e-85
Identity = 150/224 (66.96%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   WKQNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAAD 65
           W Q CLGALDGT+IKV+VS ++RPRYR+RKG+ITTNVLGV    GEFIFVMP WEGSA+D
Sbjct: 90  WFQKCLGALDGTHIKVNVSMSDRPRYRSRKGDITTNVLGVCLQNGEFIFVMPGWEGSASD 149

Query: 66  SRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPRE 125
           SR+LRDA+SR  GL V KGYYYLCD GYPNAEGFLAPYRG+RYHLTEWR GGN P  P+E
Sbjct: 150 SRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLTEWR-GGNPPKCPKE 209

Query: 126 FFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDV 185
            FNM+HS ARNVIERAF +L  RW IL+G+SYY V  QC+IITACCLLHNLI REMG + 
Sbjct: 210 LFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLVDIQCKIITACCLLHNLIHREMGSEA 269

Query: 186 GLDEGDIGRSEPIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
              E  +G  +   ++ ENI F++++  WT+ RD+LAN+MF  W
Sbjct: 270 TFKEPHLGEGDSSEMNIENINFVETTNVWTEWRDNLANQMFEDW 312

[Close]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL
Match: A0A5A7TWL4 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001690 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 5.4e-85
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIK +V A +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 101 CNDQRWKCFENCLGALDGTYIKANVPAGDRPTFRTRKGEIATNVLGVCDTKGDFVYVIAG 160

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 161 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 220

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAFD L+GRW ILRGKSYYP++ QCR I AC LLHNLI 
Sbjct: 221 APTNAKEYFNMKHSSARNVIERAFDVLKGRWAILRGKSYYPLQVQCRTILACALLHNLIN 280

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 281 REMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 332

[Close]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL
Match: A0A5A7VE61 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold99G00190 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 9.1e-85
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIKV+V   +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 101 CNDQRWKCFENCLGALDGTYIKVNVPEGDRPTFRTRKGEIATNVLGVCDTKGDFVYVLVS 160

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 161 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 220

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAF  L+GRW ILRGKSYYP++ QCRII AC LLHNLI 
Sbjct: 221 APTNAKEYFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPLQVQCRIILACALLHNLIN 280

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 281 REMTYCNDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 332

[Close]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL
Match: A0A5A7UST8 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold288G00660 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 9.1e-85
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIKV+V A +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 126 CNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFRTRKGEIATNVLGVCDTKGDFVYVLAG 185

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 186 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 245

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAF  L+GRW ILRGKSYYP++ QCR I AC LLHNLI 
Sbjct: 246 APTNAKEYFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPLQVQCRTILACALLHNLIN 305

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 306 REMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 357

[Close]

BLAST of Lcy06g016870 vs. NCBI nr
Match: XP_008455792.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 327.4 bits (838), Expect = 1.0e-85
Identity = 157/224 (70.09%), Postives = 175/224 (78.12%), Query Frame = 0


Query: 6   WKQNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAAD 65
           W +NCLGALDGTYIKV+VSA +RPRYRTRKGE+ TNVLG   +KG+F+FV+  WEGSAAD
Sbjct: 94  WFENCLGALDGTYIKVNVSATDRPRYRTRKGEVATNVLGACDTKGDFVFVLFGWEGSAAD 153

Query: 66  SRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPRE 125
           SR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRGERYHL+EWRG  NAPTT RE
Sbjct: 154 SRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFLAPYRGERYHLSEWRGESNAPTTARE 213

Query: 126 FFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDV 185
           FFNMKHSSARNVIERAF  L+GRW ILRGKSYYPV  QCR I ACCLLHNLI REM    
Sbjct: 214 FFNMKHSSARNVIERAFGLLKGRWAILRGKSYYPVDVQCRTIMACCLLHNLINREM---- 273

Query: 186 GLDEGDIGRSEPIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
                    SE I      I +I++S EW++ RD LA+ MF+ W
Sbjct: 274 -------TNSEII-----EINYIEASNEWSEWRDQLAHTMFSDW 301

[Close]

BLAST of Lcy06g016870 vs. NCBI nr
Match: KAA0065306.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 324.3 bits (830), Expect = 8.5e-85
Identity = 150/224 (66.96%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   WKQNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAAD 65
           W Q CLGALDGT+IKV+VS ++RPRYR+RKG+ITTNVLGV    GEFIFVMP WEGSA+D
Sbjct: 90  WFQKCLGALDGTHIKVNVSMSDRPRYRSRKGDITTNVLGVCLQNGEFIFVMPGWEGSASD 149

Query: 66  SRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPRE 125
           SR+LRDA+SR  GL V KGYYYLCD GYPNAEGFLAPYRG+RYHLTEWR GGN P  P+E
Sbjct: 150 SRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLTEWR-GGNPPKCPKE 209

Query: 126 FFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDV 185
            FNM+HS ARNVIERAF +L  RW IL+G+SYY V  QC+IITACCLLHNLI REMG + 
Sbjct: 210 LFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLVDIQCKIITACCLLHNLIHREMGSEA 269

Query: 186 GLDEGDIGRSEPIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
              E  +G  +   ++ ENI F++++  WT+ RD+LAN+MF  W
Sbjct: 270 TFKEPHLGEGDSSEMNIENINFVETTNVWTEWRDNLANQMFEDW 312

[Close]

BLAST of Lcy06g016870 vs. NCBI nr
Match: KAA0047892.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 323.9 bits (829), Expect = 1.1e-84
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIK +V A +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 101 CNDQRWKCFENCLGALDGTYIKANVPAGDRPTFRTRKGEIATNVLGVCDTKGDFVYVIAG 160

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 161 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 220

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAFD L+GRW ILRGKSYYP++ QCR I AC LLHNLI 
Sbjct: 221 APTNAKEYFNMKHSSARNVIERAFDVLKGRWAILRGKSYYPLQVQCRTILACALLHNLIN 280

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 281 REMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 332

[Close]

BLAST of Lcy06g016870 vs. NCBI nr
Match: KAA0064021.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 323.2 bits (827), Expect = 1.9e-84
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIKV+V   +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 101 CNDQRWKCFENCLGALDGTYIKVNVPEGDRPTFRTRKGEIATNVLGVCDTKGDFVYVLVS 160

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 161 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 220

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAF  L+GRW ILRGKSYYP++ QCRII AC LLHNLI 
Sbjct: 221 APTNAKEYFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPLQVQCRIILACALLHNLIN 280

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 281 REMTYCNDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 332

[Close]

BLAST of Lcy06g016870 vs. NCBI nr
Match: KAA0056561.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 323.2 bits (827), Expect = 1.9e-84
Identity = 154/232 (66.38%), Postives = 179/232 (77.16%), Query Frame = 0


Query: 2   CNSM-WK--QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPR 61
           CN   WK  +NCLGALDGTYIKV+V A +RP +RTRKGEI TNVLGV  +KG+F++V+  
Sbjct: 126 CNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFRTRKGEIATNVLGVCDTKGDFVYVLAG 185

Query: 62  WEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGN 121
           WEGSAADSR+LRDAISR NGL V KGYYYLCD GYPNAEGFLAPYRG+RYHL EWRG  N
Sbjct: 186 WEGSAADSRILRDAISRENGLQVPKGYYYLCDAGYPNAEGFLAPYRGQRYHLQEWRGAAN 245

Query: 122 APTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLIT 181
           APT  +E+FNMKHSSARNVIERAF  L+GRW ILRGKSYYP++ QCR I AC LLHNLI 
Sbjct: 246 APTNAKEYFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPLQVQCRTILACALLHNLIN 305

Query: 182 REMGLDVGLDEGDIGRSE-PIPLDGENITFIQSSTEWTQKRDDLANRMFNAW 230
           REM     +++ D G S        E+I +I+++ EW+Q RDDLA  MF  W
Sbjct: 306 REMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFTDW 357

[Close]

BLAST of Lcy06g016870 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 124.4 bits (311), Expect = 1.2e-28
Identity = 71/239 (29.71%), Postives = 116/239 (48.54%), Query Frame = 0


Query: 8   QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSR 67
           ++C+G +D  +I V V    +  +R   G +T NVL   S    F +V+  WEGSA+D +
Sbjct: 141 KDCVGVVDSFHIPVMVGVDEQGPFRNGNGLLTQNVLAASSFDLRFNYVLAGWEGSASDQQ 200

Query: 68  LLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLTEWRGGGNAPTTPREFF 127
           +L  A++R N L V +G YY+ D  YPN  GF+APY G            N+    +E F
Sbjct: 201 VLNAALTRRNKLQVPQGKYYIVDNKYPNLPGFIAPYHGV---------STNSREEAKEMF 260

Query: 128 NMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACCLLHNLITREMGLDVGL 187
           N +H      I R F  L+ R+ IL     YP++TQ +++ A C LHN +  E   D+  
Sbjct: 261 NERHKLLHRAIHRTFGALKERFPILLSAPPYPLQTQVKLVIAACALHNYVRLEKPDDLVF 320

Query: 188 ------DEGDIGRSEPIPLDGENITFI--------QSSTEWTQKRDDLANRMFNAWGQH 233
                    + G    + L+ E +  +        +   +  + RD++A+ ++N + Q+
Sbjct: 321 RMFEEETLAEAGEDREVALEEEQVEIVGQEHGFRPEEVEDSLRLRDEIASELWNHYVQN 370

[Close]

BLAST of Lcy06g016870 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 117.1 bits (292), Expect = 1.9e-26
Identity = 61/152 (40.13%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 52  FIFVMPRWEGSAADSRLLRDAISRPNGLIVLKGYYYLCDVGYPNAEGFLAPYRGERYHLT 111
           FI+V+  WEGSA DSR+L DA+ +          +YL D G+ N   FLAP+RG RYHL 
Sbjct: 25  FIYVLSGWEGSAHDSRVLSDALRK----------FYLVDCGFANRLNFLAPFRGVRYHLQ 84

Query: 112 EWRGGGNAPTTPREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQCRIITACC 171
           E+ G    P TP E FN++H S RNVIER F   + R+ I +    +  + Q  ++  C 
Sbjct: 85  EFAGQRRDPETPHELFNLRHVSLRNVIERIFGIFKSRFAIFKSAPPFSYKKQAGLVLTCA 144

Query: 172 LLHNLITREMGLD-------VGLDEGDIGRSE 197
            LHN + +E   D       VG +EGD+  +E
Sbjct: 145 ALHNFLRKECRSDEADFPDEVG-NEGDVVNNE 165

[Close]

BLAST of Lcy06g016870 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-15
Identity = 47/138 (34.06%), Postives = 70/138 (50.72%), Query Frame = 0


Query: 11  LGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSRLLR 70
           +GA+DGT++ V V    +  Y  R    + N++ +   K  F ++     GS  D+ +L+
Sbjct: 177 VGAMDGTHVCVKVKPDLQGMYWNRHDNASLNIMAICDLKMLFTYIWNGAPGSCYDTAVLQ 236

Query: 71  DAISRPNGL-IVLKGYYYLCDVGYPNAEGFLAPYRGE-----RYHLTEWRGGGNAPTTPR 130
            A    +   +     YYL D GYPN +G LAPYR       RYH++++  G   P    
Sbjct: 237 IAQQSDSEFPLPPSEKYYLVDSGYPNKQGLLAPYRSSRNRVVRYHMSQFYYGPR-PRNKH 296

Query: 131 EFFNMKHSSARNVIERAF 143
           E FN  H+S R+VIER F
Sbjct: 297 ELFNQCHTSLRSVIERTF 313

[Close]

BLAST of Lcy06g016870 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-13
Identity = 57/192 (29.69%), Postives = 83/192 (43.23%), Query Frame = 0


Query: 9   NCLGALDGTYIKVHVSAANRPRYRTRKGE--ITTNVLGVVSSKGEFIFVMPRWEGSAADS 68
           NC GA+D T+I +++ A          GE   +  +  VV     F+ V+  W GS  D 
Sbjct: 182 NCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLND- 241

Query: 69  RLLRDAISRPNGL--IVLKG-----------------YYYLCDVGYPNAEGFLAPYRGER 128
               D + + +G   +V KG                  Y + D G+P     L PY+G+ 
Sbjct: 242 ----DVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGK- 301

Query: 129 YHLTEWRGGGNAPTT-PREFFNMKHSSARNVIERAFDTLRGRWVILRGKSYYPVRTQC-R 178
                       PT+ P+  FN +HS A    + A   L+ RW I+ G  + P R +  R
Sbjct: 302 ------------PTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPR 355

[Close]

BLAST of Lcy06g016870 vs. TAIR 10
Match: AT5G28950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 448 Blast hits to 446 proteins in 74 species: Archae - 0; Bacteria - 0; Metazoa - 31; Fungi - 21; Plants - 396; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 2.1e-12
Identity = 30/68 (44.12%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 8  QNCLGALDGTYIKVHVSAANRPRYRTRKGEITTNVLGVVSSKGEFIFVMPRWEGSAADSR 67
          ++C+GA+D T+I   VS    P +R RKG+I+ N+L   +   EF++V+  WEGSA DS+
Sbjct: 23 KDCVGAIDDTHIFAMVSQKKMPSFRNRKGDISQNMLAACNFDVEFMYVLSGWEGSAHDSK 82

Query: 68 LLRDAISR 76
          +L DA++R
Sbjct: 83 VLNDALTR 90

The following BLAST results are available for this feature:

BLAST of Lcy06g016870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U3	2.0e-12	29.69	Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1	[more]
Q96MB7	9.9e-12	30.56	Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1	[more]
Q17QR8	2.9e-11	30.56	Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1	[more]
B0BN95	4.2e-10	29.44	Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1	[more]
Q8BR93	2.3e-08	28.33	Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1	[more]

BLAST of Lcy06g016870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C1U8	4.8e-86	70.09	putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1	[more]
A0A5A7VG45	4.1e-85	66.96	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7TWL4	5.4e-85	66.38	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7VE61	9.1e-85	66.38	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7UST8	9.1e-85	66.38	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of Lcy06g016870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455792.1	1.0e-85	70.09	PREDICTED: putative nuclease HARBI1 [Cucumis melo]	[more]
KAA0065306.1	8.5e-85	66.96	retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0047892.1	1.1e-84	66.38	retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0064021.1	1.9e-84	66.38	retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0056561.1	1.9e-84	66.38	retrotransposon protein [Cucumis melo var. makuwa]	[more]

BLAST of Lcy06g016870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41980.1	1.2e-28	29.71	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT5G35695.1	1.9e-26	40.13	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT1G43722.1	2.0e-15	34.06	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G55350.1	1.4e-13	29.69	PIF / Ping-Pong family of plant transposases	[more]
AT5G28950.1	2.1e-12	44.12	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027806	Harbinger transposase-derived nuclease domain	PFAM	PF13359	DDE_Tnp_4	coord: 14..175 e-value: 4.5E-25 score: 88.2
None	No IPR available	PANTHER	PTHR22930	UNCHARACTERIZED	coord: 6..178
None	No IPR available	PANTHER	PTHR22930:SF211	NUCLEASE HARBI1-RELATED	coord: 6..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy06g016870.1	Lcy06g016870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding