Lcy06g012250 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g012250
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionThymidine kinase
LocationChr06: 15672272 .. 15675572 (+)
RNA-Seq ExpressionLcy06g012250
SyntenyLcy06g012250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGATTCCCGCTCCAAATCGAAGAACCCTCATTGTTCAACAATTTACAGAGAGACATTTCTCAACCCTTTCATAAAACCCCATTAAAACCCTCATCGACAAAGTTCATGTTAATCATTTCAAAGATGAAATCCTTCGTATCTTCCTCGTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTTCCGCTTCTCCAACATTCTTCTCCTTACCTTCTAAATCGACGCAATGCAACCCTATATTCACCCAATTCTCCAATCATTTCACTTCCAAAACGCCCTCAATTTCCTTCCCCACCAAAGGGCTTTTTTCAACCCACAATCGAACTCTGCAAATGGGAGCTTCTTTGTCTCCGCCCTCCGGCCAGGTTCATGTCATAGTGGGCCCGATGTTCGCTGGGAAAACAACCACTCTTCTTCAGCGGATTCAGTCCGAGAGCAGCCATGGCAGGTCTTTTATGCTTTGCTTTTCTGCTTCTCTTTATTAATCGAGTGGGGTGTGAGTGTTTAGTGTTATGTTTCTTCTAGCCTCGTTTGTTTGAACTTTTCTCCCTTGTATTTGTAGCTCTTTGCGATTAATGGAAATGGGGTTTACACGTATGATAGTAGATGGTAAGGTAGGTGATAGAGTGGGAGAATTTTAATCTTTTGATGTATTCTCGACACTCTCTCTGTATGGGCTTGAATATTGGCATTAGGGTATTGATGTGGAATGGGAAGAAAATGGCATTGTAAGGTTTTGAACTTGGAACCTCTTGTGCTATATTTTACTTAAATCGCTACTGATATTGAAAGTTTGGGTGGATGGGTTATGATAACAAATTCTCAACAATAGAGTTACGTATATTGGTGATAAACAAGGTTTTAAACTGATATATAACGTGGGATTTGGCCTCTCGAGAACGAATGAGTTAAACTGGTGAATCGGCAAACTGAAGTAACAGATTCTTGGGGGAAAAGCTCTAGAAAGAAGCACAAAAAGAATTGAATCCCAAGCTTTCCCTTGCTTCCTTAGCTCTTCTGAAAAATTCTTCCCTTTTTTAGCTTAACACACTAGAAGATAGCCTATTTACATTAGAGCACATGACCTTGGCTGTTTCTTTGAAAGAAAACTGCTGATATGTAGACATTGTTGCAGAGAAAATTTCACACCAAAGGTCTAGGACAGGTTCTACTCACCTGTAAAATAAAGCGACATTTGAGAAGTGGAGTTGGTAACTTGTATTTTCACCATGCCTTCTGCAAAGTATGTGCCAGTTCAGTTGGATTGTACAATTTAGACATCTAGTTTGAATCTTATCATTAGTGTTTATGGATAAGCTTCCTGCTATTCATGTATATCAGAGAAAGAGAGTGAAGGGAGGGACCCAATTAGTAGCTAGCGTGGGTAAATTCTTAGGGCTACACTGATAGCTATGTGATGCAAGTAATGGAAGGACTAGGAAAGATGGGACTACAAATAGTATTTGGCAAAGATAGAGTACAAGACCTTGGTTCAAGTGCTCTTTGTTCAAACCCCTTTGTTTTTGTCTTCTTTTGGTGTGTCATATTCAAGACCTCTTGTTATCTAGCAAGTAAACCCCTTTTTTAGATTTTCATTAATTAGATTTTGTTTTTCTTTTTTTGGGGTACCCTCTCCTTTAGTTTGTTAGGATGACATCTTGTCTTCCCTATTCTTTTTCTTTTGTCCTGTTTAATGATTTGTATCTTTATAGTATTAGATTGCCCTATCTTTTTATATTGATGGTCAAGGCAGCATCTTTCTGCTGAAGGCTTAAGCTCTACATGCTTGTTGTTGGTTGCATATAAATATGTCTTGAAAATTTTAGAACATCAACCTTGTGATTACTTGGATGGAATGTCTGATGTATCTTGATTGGTGTAGAAGCTTTCTTTTTTTGGGTACAATTTATAAGAATTATTCTTTTTTCTTTTTATGCATGTTTTTTACAGAAGTGTAGCTATAATTAAGTCAAATAAGGACACGAGATATGGATTGGATTCGATTGTGACGCATGACGGCTTGAAACTTCCATGCTGGGCATTGCCAAGCTTGTCATCTTTCAAACAAAAATTTGGTCAAGGTGCTTATGACAAGGTATCAGAATTCCTTATGATGTCAATGTACATGACTATACATTTTATCAATTCAGAAGCCTTTTGAGTTTTCAGTGACCATTTTTTTAATTGAACTTCGTTGTTTTCCTGGTCCGCTAAATGGTTAATTTACTTATGGTATTGGTGTGACAGCTAGACGTGATTGGAATCGATGAAGCTCAATTCTTTGATGATCTTTATGATTTCTGTCGCGAAGCTGCCGATATTGATGGCAAAACGGTTATAGTTGCTGGGTTGGATGGGGACTACTTGAGGTATTATCAAATTTACTGCCATAGTTTTTGTTCATTCGTTCAAATAATATCAATGCTTTTCTTAAAAGGTGACGACCTTTCAGATTGATTTAAATTTACACATCAGTAACTCAGTTAAAGCTTTTGTGTTGGGGCTTATGCAAAATCTGAACAGGAGGAGCTTCGGTTCAGTTCTTGATATAATTCCACTTGCTGATTCTGTAACAAAGTTAACTGCTCAATGTGAGATATGCGGGAACCGAGCTTTCTTTACTTTAAGAAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGCGCAGACGTGTATATGCCGGTATGTCGACAACATTATGTCAGCGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTTGAATCACACAAGGTTGAATGTAGGAGCCCAGCATAGTTGTTTTTAGTAGTAGCTCTGCAGAATCACATACATGCTCGGTAGCAAATGCCGAAACATATTTGTTGTCTCGTCAATTAAGTTAACTAGGGATCGGGCGATTTTGGAAACGGAGGGAATTATAGTAAGCGTAAGTATAATGTTAGGATTAAGGATATTTACTCAAAGAATTGAAGGCAAGAATGTAGAAGGAAGTGAAAGGGTCTTGTCTTGTAGGTTTGGAAAATGGAGTTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGTTTGATTGATTGGATGAGAGGGAAAAATGAGTCAATGTTGTGTCATATGTATTTGTGTTGGTTGAATGTAACCAACAAATTGAAAAATGAGAGGGAAAAATGAGTCAATGTTGTGTCATATGTATTTGTGTTGGTTGAATGTAACCAACAAAATTGAAAACTCATTCATTATTGGGGGAGGAGACATTCAAGTGGCAACCATGTGTGTGCTTGGTAAGCTGTTGATCAGCCAGCTGTTTATTGTATTGTGAAGA

mRNA sequence

GCCGATTCCCGCTCCAAATCGAAGAACCCTCATTGTTCAACAATTTACAGAGAGACATTTCTCAACCCTTTCATAAAACCCCATTAAAACCCTCATCGACAAAGTTCATGTTAATCATTTCAAAGATGAAATCCTTCGTATCTTCCTCGTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTTCCGCTTCTCCAACATTCTTCTCCTTACCTTCTAAATCGACGCAATGCAACCCTATATTCACCCAATTCTCCAATCATTTCACTTCCAAAACGCCCTCAATTTCCTTCCCCACCAAAGGGCTTTTTTCAACCCACAATCGAACTCTGCAAATGGGAGCTTCTTTGTCTCCGCCCTCCGGCCAGGTTCATGTCATAGTGGGCCCGATGTTCGCTGGGAAAACAACCACTCTTCTTCAGCGGATTCAGTCCGAGAGCAGCCATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAGGACACGAGATATGGATTGGATTCGATTGTGACGCATGACGGCTTGAAACTTCCATGCTGGGCATTGCCAAGCTTGTCATCTTTCAAACAAAAATTTGGTCAAGGTGCTTATGACAAGCTAGACGTGATTGGAATCGATGAAGCTCAATTCTTTGATGATCTTTATGATTTCTGTCGCGAAGCTGCCGATATTGATGGCAAAACGGTTATAGTTGCTGGGTTGGATGGGGACTACTTGAGGAGGAGCTTCGGTTCAGTTCTTGATATAATTCCACTTGCTGATTCTGTAACAAAGTTAACTGCTCAATGTGAGATATGCGGGAACCGAGCTTTCTTTACTTTAAGAAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGCGCAGACGTGTATATGCCGGTATGTCGACAACATTATGTCAGCGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTTGAATCACACAAGGTTGAATGTAGGAGCCCAGCATAGTTGTTTTTAGTAGTAGCTCTGCAGAATCACATACATGCTCGGTAGCAAATGCCGAAACATATTTGTTGTCTCGTCAATTAAGTTAACTAGGGATCGGGCGATTTTGGAAACGGAGGGAATTATAGTAAGCGTAAGTATAATGTTAGGATTAAGGATATTTACTCAAAGAATTGAAGGCAAGAATGTAGAAGGAAGTGAAAGGGTCTTGTCTTGTAGGTTTGGAAAATGGAGTTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGTTTGATTGATTGGATGAGAGGGAAAAATGAGTCAATGTTGTGTCATATGTATTTGTGTTGGTTGAATGTAACCAACAAATTGAAAAATGAGAGGGAAAAATGAGTCAATGTTGTGTCATATGTATTTGTGTTGGTTGAATGTAACCAACAAAATTGAAAACTCATTCATTATTGGGGGAGGAGACATTCAAGTGGCAACCATGTGTGTGCTTGGTAAGCTGTTGATCAGCCAGCTGTTTATTGTATTGTGAAGA

Coding sequence (CDS)

ATGTTAATCATTTCAAAGATGAAATCCTTCGTATCTTCCTCGTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTTCCGCTTCTCCAACATTCTTCTCCTTACCTTCTAAATCGACGCAATGCAACCCTATATTCACCCAATTCTCCAATCATTTCACTTCCAAAACGCCCTCAATTTCCTTCCCCACCAAAGGGCTTTTTTCAACCCACAATCGAACTCTGCAAATGGGAGCTTCTTTGTCTCCGCCCTCCGGCCAGGTTCATGTCATAGTGGGCCCGATGTTCGCTGGGAAAACAACCACTCTTCTTCAGCGGATTCAGTCCGAGAGCAGCCATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAGGACACGAGATATGGATTGGATTCGATTGTGACGCATGACGGCTTGAAACTTCCATGCTGGGCATTGCCAAGCTTGTCATCTTTCAAACAAAAATTTGGTCAAGGTGCTTATGACAAGCTAGACGTGATTGGAATCGATGAAGCTCAATTCTTTGATGATCTTTATGATTTCTGTCGCGAAGCTGCCGATATTGATGGCAAAACGGTTATAGTTGCTGGGTTGGATGGGGACTACTTGAGGAGGAGCTTCGGTTCAGTTCTTGATATAATTCCACTTGCTGATTCTGTAACAAAGTTAACTGCTCAATGTGAGATATGCGGGAACCGAGCTTTCTTTACTTTAAGAAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGCGCAGACGTGTATATGCCGGTATGTCGACAACATTATGTCAGCGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTTGAATCACACAAGGTTGAATGTAGGAGCCCAGCATAG

Protein sequence

MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSISFPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKVECRSPA
Homology
BLAST of Lcy06g012250 vs. ExPASy Swiss-Prot
Match: O81263 (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 4.1e-84
Identity = 148/197 (75.13%), Postives = 170/197 (86.29%), Query Frame = 0

Query: 85  GQVHVIVGPMFAGKTTTLLQRIQSESSHG-RSVAIIKSNKDTRYGLDSIVTHDGLKLPCW 144
           G++HVIVGPMFAGKTT LL+R+Q E+  G R+VA+IKS+KD RYGLDS+VTHDG K+PCW
Sbjct: 67  GEIHVIVGPMFAGKTTALLRRVQVEAGTGSRNVALIKSDKDNRYGLDSVVTHDGTKMPCW 126

Query: 145 ALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 204
           ALP LSSF+ K G  AYDK+DVIGIDEAQFFDDL+DFC +AAD DGK V+VAGLDGDY R
Sbjct: 127 ALPELSSFQDKLGTEAYDKVDVIGIDEAQFFDDLHDFCCKAADRDGKIVVVAGLDGDYKR 186

Query: 205 RSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 264
             FGSVLDIIPLADSVTKLTA+CE+CG RAFFTLRKT E +TELIGGADVYMPVCRQHY+
Sbjct: 187 NKFGSVLDIIPLADSVTKLTARCELCGRRAFFTLRKTRETKTELIGGADVYMPVCRQHYL 246

Query: 265 SGQVVIETARTVVESHK 281
            GQ+VIE  R V++  K
Sbjct: 247 DGQIVIEATRIVLDLEK 263

BLAST of Lcy06g012250 vs. ExPASy Swiss-Prot
Match: F4KBF5 (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.1e-81
Identity = 164/274 (59.85%), Postives = 204/274 (74.45%), Query Frame = 0

Query: 7   MKSFVSSSSFSTLSPYFPISASPTFFSLPSK-STQCNPIFTQFSNHFTSKTPSISFPTKG 66
           M++ +S S    L+P+      P+ FS   + S   N I    S   T  T  +   TK 
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQ--TKA 65

Query: 67  LFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDT 126
              T + + Q  +S SP  G++HV+VGPMF+GKTTTLL+RI +E   G+ +AIIKSNKDT
Sbjct: 66  TRVTSSSSSQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP LSSFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVLD+IP+AD+VTKLT++CE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

BLAST of Lcy06g012250 vs. ExPASy Swiss-Prot
Match: Q9S750 (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 4.7e-80
Identity = 139/194 (71.65%), Postives = 169/194 (87.11%), Query Frame = 0

Query: 84  SGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTRYGLDSIVTHDGLKLPCW 143
           SG VHVI+GPMF+GK+T+LL+RI+SE S GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           RSFG+VLDIIP+ADSVTKLTA+CE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

BLAST of Lcy06g012250 vs. ExPASy Swiss-Prot
Match: P23335 (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-37
Identity = 79/180 (43.89%), Postives = 115/180 (63.89%), Query Frame = 0

Query: 85  GQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTRYGLDSIVTHDGLKLPCWA 144
           G +H+I+GPMF+GK+T L++ +        +  +IK +KD RYG D++ THD   +   +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 145 LPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRR 204
             SL   K        D +D++GIDE QFF+D+ +FC   A+  GK VIVA LDG Y R+
Sbjct: 64  TDSLFDIKD-----TLDDVDIVGIDEGQFFNDIVEFCEYIAN-KGKIVIVAALDGTYERK 123

Query: 205 SFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVS 264
            FG++L++IPL++ VTKL A C IC   A F+ R ++EKE ELIGG + Y+ VCR  Y++
Sbjct: 124 PFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCYLT 177

BLAST of Lcy06g012250 vs. ExPASy Swiss-Prot
Match: P04184 (Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3)

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-34
Identity = 86/185 (46.49%), Postives = 112/185 (60.54%), Query Frame = 0

Query: 78  ASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTRYGLDSIVTHDG 137
           +S S   GQ+ VI+GPMF+GK+T L++R++          +IK  KDTRY  +S  THD 
Sbjct: 12  SSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYS-NSFSTHDR 71

Query: 138 LKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGL 197
             +       L    Q+    A     VIGIDE QFF D+ DFC   A+ +GKTVIVA L
Sbjct: 72  NTMDALPACMLRDVTQESLGVA-----VIGIDEGQFFPDIVDFCEMMAN-EGKTVIVAAL 131

Query: 198 DGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPV 257
           DG + R++FGS+L+++PLA+SV KLTA C  C   A +T R   EKE E+IGGAD Y  V
Sbjct: 132 DGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSV 189

Query: 258 CRQHY 263
           CR  Y
Sbjct: 192 CRLCY 189

BLAST of Lcy06g012250 vs. ExPASy TrEMBL
Match: A0A0A0LYL4 (Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.2e-136
Identity = 245/284 (86.27%), Postives = 264/284 (92.96%), Query Frame = 0

Query: 4   ISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSISFPT 63
           IS MKSFV SSSFS LSPYFPISASP+FFSLPSKS QC P+FTQ SN FT KTP+IS P+
Sbjct: 70  ISNMKSFVFSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQLSNFFTFKTPTISLPS 129

Query: 64  KGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNK 123
           KG FST NR  QM ASLSPPSG++HVI+GPMFAGKTTTLL+RIQSES +GRSVAIIKSNK
Sbjct: 130 KGFFSTQNRNPQMRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNK 189

Query: 124 DTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCRE 183
           DTRYGLDSIVTHDG+KLPCWA+P+LSSFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC E
Sbjct: 190 DTRYGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCE 249

Query: 184 AADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEK 243
           AADIDGKTVIVAGLDGDYLRR+FGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT+EK
Sbjct: 250 AADIDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEK 309

Query: 244 ETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKVECRSPA 288
           ETELIGGAD+YMPVCRQHYVSGQV IETARTVVES KV  R+PA
Sbjct: 310 ETELIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

BLAST of Lcy06g012250 vs. ExPASy TrEMBL
Match: A0A1S4E495 (Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.4e-135
Identity = 243/281 (86.48%), Postives = 262/281 (93.24%), Query Frame = 0

Query: 7   MKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSISFPTKGL 66
           MKS VSSSSFS LSPYFPISASP+FFSLPSKS QC P+FTQ SN FT KTP+ S P+KG 
Sbjct: 1   MKSLVSSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQVSNFFTFKTPTTSLPSKGF 60

Query: 67  FSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTR 126
           FST NR  Q  ASLSPPSG++HVI+GPMFAGKTTTLL+RIQSES +GR+VAIIKSNKDTR
Sbjct: 61  FSTQNRNPQTRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDG+KLPCWA+P+LSSFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRR+FGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKVECRSPA 288
           LIGGADVYMPVCRQHYVSGQVVIE ARTVVES KVECR+PA
Sbjct: 241 LIGGADVYMPVCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

BLAST of Lcy06g012250 vs. ExPASy TrEMBL
Match: A0A6J1HQ68 (Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111466371 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.7e-133
Identity = 249/288 (86.46%), Postives = 265/288 (92.01%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMKSFVSSSSFSTLSPYFPI+ASPT  SL  KSTQCNPIFT  SN F+ KTP +S
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLAYKSTQCNPIFTHLSNFFSLKTPRVS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P    FST  RT QMGASLSPPSG+VHVIVGPMFAGKTT+LL+RIQSES +GRSVAIIK
Sbjct: 61  LPRNEGFSTQKRTAQMGASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWA+P+LSSFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWAVPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKVECRSPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HKVE  + A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

BLAST of Lcy06g012250 vs. ExPASy TrEMBL
Match: A0A6J1EBD4 (Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111432595 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.0e-130
Identity = 245/288 (85.07%), Postives = 263/288 (91.32%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMKSFVSSSSFSTLSPYFPI+ASPT  SL SKSTQCNPIFT  SN F+ KT  +S
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTLRVS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P    FS   R+ Q+ ASLSPPSG+VHVIVGPMFAGKTT+LL+RIQSES +GRSVA+IK
Sbjct: 61  LPRNQGFSNQKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAVIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWALP+LSSFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKVECRSPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HKVE  + A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

BLAST of Lcy06g012250 vs. ExPASy TrEMBL
Match: A0A6J1CKS9 (Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111012527 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.9e-130
Identity = 243/290 (83.79%), Postives = 259/290 (89.31%), Query Frame = 0

Query: 1   MLIISKMKSFV-SSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSI 60
           M  IS+MK FV SSSSFSTL P  PI+  P FFSL SK T C PIF+Q  N FT KTP+I
Sbjct: 3   MSTISRMKPFVSSSSSFSTLFPGIPITTPPPFFSLLSKPTHCRPIFSQPVNFFTFKTPTI 62

Query: 61  SFPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAII 120
           S P  G+FST NRT  +GAS SPPSG+VHVIVGPMFAGKTTTLL+RIQSESS GRSVAII
Sbjct: 63  SLPINGVFSTQNRTGPIGASSSPPSGEVHVIVGPMFAGKTTTLLRRIQSESSDGRSVAII 122

Query: 121 KSNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYD 180
           KSNKDTRYGLDSIVTHDG+KLPCWALP+LSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYD
Sbjct: 123 KSNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYD 182

Query: 181 FCREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRK 240
           FCREAADIDGKT+IV+GLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRK
Sbjct: 183 FCREAADIDGKTIIVSGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRK 242

Query: 241 TEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESH--KVECRSPA 288
           TEEKETELIGGADVYMPVCR+HYVSGQV IE ARTV+ESH  KVEC +PA
Sbjct: 243 TEEKETELIGGADVYMPVCRRHYVSGQVAIEAARTVIESHQDKVECGNPA 292

BLAST of Lcy06g012250 vs. NCBI nr
Match: XP_038893570.1 (thymidine kinase a-like [Benincasa hispida])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 253/287 (88.15%), Postives = 271/287 (94.43%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMK FVSSSS STLSPYFPI+ASP+FFSLPSKSTQC P FT  SN+ T KTP IS
Sbjct: 1   MLTISKMKPFVSSSSVSTLSPYFPITASPSFFSLPSKSTQCKPTFTHVSNYLTFKTPIIS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P+KG+FST NR  QM AS SPPSG++HVIVGPMFAGKTTTLL+RIQSESS+GRSVAIIK
Sbjct: 61  LPSKGVFSTQNRIGQMKASFSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWALP+L+SFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAADIDGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKVECRSPA 288
           EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHK+EC++PA
Sbjct: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKIECQTPA 287

BLAST of Lcy06g012250 vs. NCBI nr
Match: XP_016903056.1 (PREDICTED: thymidine kinase a-like [Cucumis melo])

HSP 1 Score: 492.3 bits (1266), Expect = 2.9e-135
Identity = 243/281 (86.48%), Postives = 262/281 (93.24%), Query Frame = 0

Query: 7   MKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSISFPTKGL 66
           MKS VSSSSFS LSPYFPISASP+FFSLPSKS QC P+FTQ SN FT KTP+ S P+KG 
Sbjct: 1   MKSLVSSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQVSNFFTFKTPTTSLPSKGF 60

Query: 67  FSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTR 126
           FST NR  Q  ASLSPPSG++HVI+GPMFAGKTTTLL+RIQSES +GR+VAIIKSNKDTR
Sbjct: 61  FSTQNRNPQTRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDG+KLPCWA+P+LSSFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRR+FGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKVECRSPA 288
           LIGGADVYMPVCRQHYVSGQVVIE ARTVVES KVECR+PA
Sbjct: 241 LIGGADVYMPVCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

BLAST of Lcy06g012250 vs. NCBI nr
Match: XP_022966756.1 (thymidine kinase a-like [Cucurbita maxima])

HSP 1 Score: 485.3 bits (1248), Expect = 3.5e-133
Identity = 249/288 (86.46%), Postives = 265/288 (92.01%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMKSFVSSSSFSTLSPYFPI+ASPT  SL  KSTQCNPIFT  SN F+ KTP +S
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLAYKSTQCNPIFTHLSNFFSLKTPRVS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P    FST  RT QMGASLSPPSG+VHVIVGPMFAGKTT+LL+RIQSES +GRSVAIIK
Sbjct: 61  LPRNEGFSTQKRTAQMGASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWA+P+LSSFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWAVPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKVECRSPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HKVE  + A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

BLAST of Lcy06g012250 vs. NCBI nr
Match: KAG6573533.1 (Thymidine kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012648.1 Thymidine kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 248/288 (86.11%), Postives = 267/288 (92.71%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMKSFVSSSSFSTLSPYFPI+ASPT  SL SKSTQCNPIFT  SN F+ KTP++S
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTPTVS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P    FSTH R+ Q+ ASLSPPSG+VHVIVGPMFAGKTT+LL+RIQSES +GRSVAIIK
Sbjct: 61  LPRNQGFSTHKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWALP+LSSFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKVECRSPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E++KVE  + A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEANKVEFTTTA 287

BLAST of Lcy06g012250 vs. NCBI nr
Match: XP_022925302.1 (thymidine kinase a-like [Cucurbita moschata])

HSP 1 Score: 476.1 bits (1224), Expect = 2.1e-130
Identity = 245/288 (85.07%), Postives = 263/288 (91.32%), Query Frame = 0

Query: 1   MLIISKMKSFVSSSSFSTLSPYFPISASPTFFSLPSKSTQCNPIFTQFSNHFTSKTPSIS 60
           ML ISKMKSFVSSSSFSTLSPYFPI+ASPT  SL SKSTQCNPIFT  SN F+ KT  +S
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTLRVS 60

Query: 61  FPTKGLFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIK 120
            P    FS   R+ Q+ ASLSPPSG+VHVIVGPMFAGKTT+LL+RIQSES +GRSVA+IK
Sbjct: 61  LPRNQGFSNQKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAVIK 120

Query: 121 SNKDTRYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDG+KLPCWALP+LSSFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTA+CEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKVECRSPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HKVE  + A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

BLAST of Lcy06g012250 vs. TAIR 10
Match: AT5G23070.1 (Thymidine kinase )

HSP 1 Score: 304.7 bits (779), Expect = 8.0e-83
Identity = 164/274 (59.85%), Postives = 204/274 (74.45%), Query Frame = 0

Query: 7   MKSFVSSSSFSTLSPYFPISASPTFFSLPSK-STQCNPIFTQFSNHFTSKTPSISFPTKG 66
           M++ +S S    L+P+      P+ FS   + S   N I    S   T  T  +   TK 
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQ--TKA 65

Query: 67  LFSTHNRTLQMGASLSPPSGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDT 126
              T + + Q  +S SP  G++HV+VGPMF+GKTTTLL+RI +E   G+ +AIIKSNKDT
Sbjct: 66  TRVTSSSSSQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGLKLPCWALPSLSSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP LSSFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVLD+IP+AD+VTKLT++CE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

BLAST of Lcy06g012250 vs. TAIR 10
Match: AT3G07800.1 (Thymidine kinase )

HSP 1 Score: 299.3 bits (765), Expect = 3.3e-81
Identity = 139/194 (71.65%), Postives = 169/194 (87.11%), Query Frame = 0

Query: 84  SGQVHVIVGPMFAGKTTTLLQRIQSESSHGRSVAIIKSNKDTRYGLDSIVTHDGLKLPCW 143
           SG VHVI+GPMF+GK+T+LL+RI+SE S GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPSLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RSFGSVLDIIPLADSVTKLTAQCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           RSFG+VLDIIP+ADSVTKLTA+CE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O812634.1e-8475.13Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2[more]
F4KBF51.1e-8159.85Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1[more]
Q9S7504.7e-8071.65Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1[more]
P233351.3e-3743.89Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1[more]
P041841.3e-3446.49Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LYL48.2e-13686.27Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1[more]
A0A1S4E4951.4e-13586.48Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1[more]
A0A6J1HQ681.7e-13386.46Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111466371 PE=3 SV=1[more]
A0A6J1EBD41.0e-13085.07Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111432595 PE=3 SV=1[more]
A0A6J1CKS93.9e-13083.79Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111012527 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038893570.11.8e-14088.15thymidine kinase a-like [Benincasa hispida][more]
XP_016903056.12.9e-13586.48PREDICTED: thymidine kinase a-like [Cucumis melo][more]
XP_022966756.13.5e-13386.46thymidine kinase a-like [Cucurbita maxima][more]
KAG6573533.11.3e-13286.11Thymidine kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012648.1 ... [more]
XP_022925302.12.1e-13085.07thymidine kinase a-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G23070.18.0e-8359.85Thymidine kinase [more]
AT3G07800.13.3e-8171.65Thymidine kinase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001267Thymidine kinasePFAMPF00265TKcoord: 85..262
e-value: 1.8E-54
score: 184.3
IPR001267Thymidine kinasePANTHERPTHR11441THYMIDINE KINASEcoord: 79..284
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 80..222
e-value: 9.2E-47
score: 160.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 85..223
NoneNo IPR availableGENE3D3.30.60.20coord: 223..264
e-value: 1.9E-17
score: 65.3
NoneNo IPR availablePANTHERPTHR11441:SF8THYMIDINE KINASE Bcoord: 79..284
NoneNo IPR availableSUPERFAMILY57716Glucocorticoid receptor-like (DNA-binding domain)coord: 224..265
IPR020633Thymidine kinase, conserved sitePROSITEPS00603TK_CELLULAR_TYPEcoord: 249..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g012250.1Lcy06g012250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009157 deoxyribonucleoside monophosphate biosynthetic process
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0046104 thymidine metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0004797 thymidine kinase activity