Lcy06g011330 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g011330
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDNA-directed RNA polymerase II subunit 4
LocationChr06: 13222400 .. 13224516 (-)
RNA-Seq ExpressionLcy06g011330
SyntenyLcy06g011330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGTGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGGTAACAATCTATATATAAATCTCTGAATTTCCCCTTCTGTGGATGGCTATTTTGCTTAGTCGTGAGAGGAGAAAGACAGTATACTTTCTTGCTATACTCTGAATTTTCCTCTCCATGTGATTATGTCTTGTAGCCATCTACTTTGGGTCGTATGTCGTAGTATCTTATAACTTTCTCAAATATTGTTAGATAAGAAATAGTCATTAGATGGAGTGTCTTATAGGTGGCTGGACGTGCACGTGTACAAAAAGAAGTTTCTTTTGTATACACCTTCTGATTGTTTTTATGATATCATTTTTTGAGTTGGCTCATTTACTACATTGAAGCACTACTTCCTTAATATATTGTTCAAGTCTCAAACAGCTAAATGCCTTGAGTTTTCTGATGTTTTGGATTAATATTATATGGTTCTAAGGTTTTCTATTTTAGATGAAGATATGTGATCTATGCAAAATCTAATTTCAGTCTGTAATCATATTGTCAAAAAGATACTTAACTATTGTAAGCTTTTCCATTTTTGTATGAATCTATGATAATGTTAGGCTATATTATAGCGAAATCTTTAGTTCACATGAATAAGTAAGTATTCAAAATTTAAGTTCTTGCCACCCAAATTTGGTGTATACTCTGTTCTTTTCCATTCTCTTGTATTTATTTCTCACTTTGTTCTTAAGAGAAAACATCAAAGGTGACAACAGACATTTATTTCCTTTGGCTGCTTGCAGAGTTCTTAAAGGTCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAATATGAGCAGCTCCAGCAGATGTCTGAGGATCCAATGAATCAAGTTTCTCAGTGAGTTTTATGTTTATTCTTGATTGATTCATAAGTTAATTTAGAGTTTGTTTTGCTGGTTGAAATAGCTAAAATATATAGCCAAAGTTATGATATTCATTATGACATGTCATATGATGAGATTTTTAATTATGGTATTCTTTCTGTAGAGTGTTCGAAAAGTCATTGCAGTACGTGAAACGTTTCAGCCGCTATAAAAATCCTGACGCTGTTAGACAAGTACGAGAGTATCCCTTAAACTGACGTCTTCTTTTACGGAATGGTGGCAAAGATGGGTTGTGCATATCTACCATATGCTATGACTGGCTCCATTTTGAACTAGTACTTTAGAACTACCATTTTCTCGCTAGCCCATAACTCTTTGGTTAGTTATTGTTTGGAAAGTGGCTAAAGTTCATTACATCTCGACAGCCATTCTATTTTATAGGTTTTTCATATGGATATAGGCTTTCCTAGGTATCCATATGCAAAAGATCGTCTATTTTGATATTTAATTCATGGTGGGAAATAGAATTTACTTTTATCACCTCAGATATGAACGGGAAGTTTGTGTTCATTGTATAGAATTGCATAAACTAGCAGTGTAATTAAACTTTTTTAAGAGGTTGAATCTTAACCGCCATACAGAATTCTAAGCAGATATCAGTTGGCCGAGTTTGAGGTATGCACTAGACACTTTGGTTTCATCAATTTACATTTGTATCTTGTATGCAGTAAAAGGAAAGATTTAATTCATCATTCTTGAAATCTGCAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTGCCATGGTTCCATCTTTGAAGGTATCTTTTGCCACATTTTTTTATGCCTAAATCAGATCAGCAAATATCGGTAGTTTTTCTGTTGCTTGTTACGGACAATATATTTATTGGTATTTTGCTAATCATAACTCATAAGTAGGTACAAACTTTTAGAAAGTTTACTGATATATTTATCTTTCTAACCTTGAACATGTGTCGAATTTTAAAATATTGCCAAAAAATTTTAAAACTTATGTAATTTGAAATTTTTAGTTTTCCCATTTCTGAAATTTGAGAGAATTCAAAAGCGTTTTCGAAGACAAAATGTTTATCGGTTGAAGTGCGATTACTTATGAATGCTTTTCTTGGGTTCAGATTAAAGGGAGGGCGCATGATGATGAAGCAATCAAGAAGATGTTGAATGACCTCTCGTTAATCAAAAAGTTTGAGTAG

mRNA sequence

ATGTCTGGTGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAGGTCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAATATGAGCAGCTCCAGCAGATGTCTGAGGATCCAATGAATCAAGTTTCTCAAGTGTTCGAAAAGTCATTGCAGTACGTGAAACGTTTCAGCCGCTATAAAAATCCTGACGCTGTTAGACAAGTACGAGAAATTCTAAGCAGATATCAGTTGGCCGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTGCCATGGTTCCATCTTTGAAGATTAAAGGGAGGGCGCATGATGATGAAGCAATCAAGAAGATGTTGAATGACCTCTCGTTAATCAAAAAGTTTGAGTAG

Coding sequence (CDS)

ATGTCTGGTGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAGGTCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAATATGAGCAGCTCCAGCAGATGTCTGAGGATCCAATGAATCAAGTTTCTCAAGTGTTCGAAAAGTCATTGCAGTACGTGAAACGTTTCAGCCGCTATAAAAATCCTGACGCTGTTAGACAAGTACGAGAAATTCTAAGCAGATATCAGTTGGCCGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTGCCATGGTTCCATCTTTGAAGATTAAAGGGAGGGCGCATGATGATGAAGCAATCAAGAAGATGTTGAATGACCTCTCGTTAATCAAAAAGTTTGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHDDEAIKKMLNDLSLIKKFE
Homology
BLAST of Lcy06g011330 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.7e-67
Identity = 128/138 (92.75%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHK+EQLQQ+SEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+AMVPSLK KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

BLAST of Lcy06g011330 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-20
Identity = 54/137 (39.42%), Postives = 92/137 (67.15%), Query Frame = 0

Query: 2   SGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF   + L+N EV ++LEH+ +Q +   ++   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIKKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

BLAST of Lcy06g011330 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-20
Identity = 54/137 (39.42%), Postives = 92/137 (67.15%), Query Frame = 0

Query: 2   SGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF   + L+N EV ++LEH+ +Q +   ++   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIKKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

BLAST of Lcy06g011330 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.2e-18
Identity = 55/147 (37.41%), Postives = 89/147 (60.54%), Query Frame = 0

Query: 4   EEEENAAELKIGDEFLKVKCLMNCEVSLILEHK----------YEQ---LQQMSEDPMNQ 63
           +EEE+   LK   +    K L+N EV+++LEH+          + Q   L  +  + +N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA +++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKIKGRAHDDEAIKKMLNDLSLIKKF 138
           SLK   +  DDE ++ +L++LS ++KF
Sbjct: 132 SLK---KMEDDE-LQAILDELSNLRKF 154

BLAST of Lcy06g011330 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 86.3 bits (212), Expect = 3.0e-16
Identity = 48/126 (38.10%), Postives = 80/126 (63.49%), Query Frame = 0

Query: 6   EENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF   + L+  EV ++L+H+  + Q  S D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHDDEAIK 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA A++PSL  +GR  +DE ++
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGR-FEDEELR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

BLAST of Lcy06g011330 vs. ExPASy TrEMBL
Match: A0A6J1BWW4 (DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC111006069 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-67
Identity = 135/138 (97.83%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. ExPASy TrEMBL
Match: A0A6J1JHK7 (DNA-directed RNA polymerase II subunit 4 OS=Cucurbita maxima OX=3661 GN=LOC111487075 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.1e-67
Identity = 134/138 (97.10%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKAKGRVHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. ExPASy TrEMBL
Match: A0A6J1GS12 (DNA-directed RNA polymerase II subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111456918 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.1e-67
Identity = 134/138 (97.10%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKAKGRVHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. ExPASy TrEMBL
Match: A0A6A1VC16 (DNA-directed RNA polymerase II subunit 4 OS=Morella rubra OX=262757 GN=CJ030_MR6G025439 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.6e-67
Identity = 133/138 (96.38%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEV+LILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+K KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. ExPASy TrEMBL
Match: A0A2N9G2U2 (RPOL4c domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21532 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.6e-67
Identity = 133/138 (96.38%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEV+LILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+K KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. NCBI nr
Match: XP_022133507.1 (DNA-directed RNA polymerase II subunit 4 [Momordica charantia])

HSP 1 Score: 265.0 bits (676), Expect = 3.6e-67
Identity = 135/138 (97.83%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. NCBI nr
Match: XP_022954766.1 (DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954778.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022990067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990068.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990070.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_023536652.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.5 bits (672), Expect = 1.1e-66
Identity = 134/138 (97.10%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKAKGRVHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. NCBI nr
Match: XP_030924038.1 (DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-directed RNA polymerase II subunit 4 [Morella rubra] >KAF3942865.1 hypothetical protein CMV_030520 [Castanea mollissima])

HSP 1 Score: 263.1 bits (671), Expect = 1.4e-66
Identity = 133/138 (96.38%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEV+LILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+K KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. NCBI nr
Match: KAF3440471.1 (hypothetical protein FNV43_RR18755 [Rhamnella rubrinervis])

HSP 1 Score: 262.7 bits (670), Expect = 1.8e-66
Identity = 133/138 (96.38%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIG+EFLK KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGEEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+K KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Lcy06g011330 vs. NCBI nr
Match: KAG8649808.1 (hypothetical protein MANES_08G141900v8 [Manihot esculenta])

HSP 1 Score: 261.9 bits (668), Expect = 3.1e-66
Identity = 132/138 (95.65%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEV+LILEHKYEQLQQMS+DPMNQVSQVFEKSLQ
Sbjct: 45  MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQ 104

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPS+K KGRAHD
Sbjct: 105 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 164

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSLIKKFE
Sbjct: 165 DEAIEKMLNDLSLIKKFE 182

BLAST of Lcy06g011330 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 256.5 bits (654), Expect = 1.2e-68
Identity = 128/138 (92.75%), Postives = 135/138 (97.83%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKVKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLK KCLMNCEVSLILEHK+EQLQQ+SEDPMNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+AMVPSLK KGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIKKMLNDLSLIKKFE 139
           DEAI+KMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

BLAST of Lcy06g011330 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 79.3 bits (194), Expect = 2.6e-15
Identity = 43/117 (36.75%), Postives = 73/117 (62.39%), Query Frame = 0

Query: 22  KCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL 81
           +C+M+CE   IL+    QL  +SEDP  ++   ++++L YV+    Y NP +VR+V E L
Sbjct: 90  ECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEPL 149

Query: 82  SRYQLAEFELCVLGNLCPETVEEAIAMVPSLKIKGRAHDDEAIKKMLNDLSLIKKFE 139
             Y +++ E+CV+ N   E+V+E +A +PSLK K +   ++ ++  L +LS +KK E
Sbjct: 150 KTYGISDGEMCVIANASSESVDEVLAFIPSLKTK-KEVINQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O488901.7e-6792.75DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
O155141.5e-2039.42DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE... [more]
Q9D7M81.5e-2039.42DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P... [more]
Q54S047.2e-1837.41DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689... [more]
Q9VEA53.0e-1638.10DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G... [more]
Match NameE-valueIdentityDescription
A0A6J1BWW41.7e-6797.83DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1JHK75.1e-6797.10DNA-directed RNA polymerase II subunit 4 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1GS125.1e-6797.10DNA-directed RNA polymerase II subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6A1VC166.6e-6796.38DNA-directed RNA polymerase II subunit 4 OS=Morella rubra OX=262757 GN=CJ030_MR6... [more]
A0A2N9G2U26.6e-6796.38RPOL4c domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21532 P... [more]
Match NameE-valueIdentityDescription
XP_022133507.13.6e-6797.83DNA-directed RNA polymerase II subunit 4 [Momordica charantia][more]
XP_022954766.11.1e-6697.10DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DN... [more]
XP_030924038.11.4e-6696.38DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-dire... [more]
KAF3440471.11.8e-6696.38hypothetical protein FNV43_RR18755 [Rhamnella rubrinervis][more]
KAG8649808.13.1e-6695.65hypothetical protein MANES_08G141900v8 [Manihot esculenta][more]
Match NameE-valueIdentityDescription
AT5G09920.11.2e-6892.75RNA polymerase II, Rpb4, core protein [more]
AT4G15950.12.6e-1536.75RNA polymerase II, Rpb4, core protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006590RNA polymerase Rpb4/RPC9, coreSMARTSM00657rpol4neu2coord: 16..138
e-value: 1.4E-56
score: 203.9
IPR005574RNA polymerase subunit RPB4/RPC9PFAMPF03874RNA_pol_Rpb4coord: 23..132
e-value: 2.5E-22
score: 79.5
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 2..138
e-value: 4.5E-50
score: 170.9
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 1..138
NoneNo IPR availablePANTHERPTHR21297:SF3DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKEcoord: 1..138
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g011330.1Lcy06g011330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0044237 cellular metabolic process
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0031369 translation initiation factor binding