Lcy06g010180 (gene) Sponge gourd (P93075) v1

BLAST of Lcy06g010180 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.0e-149
Identity = 272/539 (50.46%), Postives = 365/539 (67.72%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWI---VAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPF 60
           MK        L F   S+  L I   V+AN   +   FLQCLS    DS  +SKV++TP 
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQ-SSFLQCLSLQLNDSNIVSKVIHTPN 60

Query: 61  NSSYSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDF 120
           ++S+SSVL  +++N RFS P  PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+
Sbjct: 61  DTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 120

Query: 121 EGLSYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLC 180
           EGLSYV H PF+ILDL  L SI++DV +++ WVQ+GAT+GELYY I +K+RTLAFPAG+C
Sbjct: 121 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 180

Query: 181 PTVGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGG 240
           PTVGVGGHFSGGGYG +LRK+GLAAD+VIDA +VDA G+  +R  MGED FWAIRGGGG 
Sbjct: 181 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 240

Query: 241 SFGIVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGN 300
           SF +V++WK+ L+ VP TVTV    + SE  A  +IHRWQ+VA+K+ ++LF+ ++L    
Sbjct: 241 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML---- 300

Query: 301 FTRQEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQ 360
              Q  K    A F   YLG    ++ ++   FPEL L + DC EMSWIES +      +
Sbjct: 301 ---QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---E 360

Query: 361 PGKVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGR 420
            G+   ++L+K T  + + K KSD+V+EP+P  AI  +W RL+  + E  QLIF P+GG+
Sbjct: 361 LGEEPINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 420

Query: 421 MSQISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAA 480
           MS+I++ ETPF HR G +Y+I Y+  W  RG   EK ++ W+  +Y  M+ FV+KSPR A
Sbjct: 421 MSEIADYETPFPHRKGNIYEIQYLNYW--RGDVKEK-YMRWVERVYDDMSEFVAKSPRGA 480

Query: 481 YVNYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIP 537
           Y+N RDLD+G      ++ Y++   WG+KYF +NNF RLV VKT  DP DFF  EQSIP
Sbjct: 481 YINLRDLDLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Lcy06g010180 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 260/514 (50.58%), Postives = 347/514 (67.51%), Query Frame = 0

Query: 33  EGFLQCLSHHSPDSYSISKVVYTPFNSSYSSVLNFTLRNLRFSTPKTPKPLLIITPTNIS 92
           E FL+C S H P++ +  K+VYT  +  Y S+LN T++NLRF +  TPKPL+I+TP+N S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQAAVVCSKSHGLQIRTRSGGHDFEGLSYVAHLPFIILDLIKLNSISIDVQSKTAWVQS 152
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++ +PF+++DL  ++SI IDV S+TAWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPETVTVCFANRTSE-DGATN 272
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T+    +  E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVANKLDENLFLGILLGGGNFTRQEGKINPL--ALFFSQYLGKADEVVAILKAT 332
           L ++WQ +A K D++L L       N T   GK        F S + G  D +V ++  +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELALTKKDCIEMSWIESALIMGTGLQPGKV---LEDLLSKTTITNGSTKIKSDYVKEP 392
           FPEL + K DC E SWI++ +     +         E LL ++     +  IK DYVK+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 393 IPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQISESETPFSHRAGYMYKIGYVLTWAE 452
           IP  A+  I E+L  ED+     +  PYGG M +ISES  PF HRAG MY++ Y  +W +
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 453 RGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYKQASVWGLK 512
           +     ++H+NW+R +Y    P+VS++PR AY+NYRDLD+G  N     +Y QA +WG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 513 YFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 541
           YFG+ NFNRLV VKTK DP++FFR+EQSIP L P
Sbjct: 512 YFGK-NFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of Lcy06g010180 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.2e-147
Identity = 255/514 (49.61%), Postives = 348/514 (67.70%), Query Frame = 0

Query: 33  EGFLQCLSHHSPDSYSISKVVYTPFNSSYSSVLNFTLRNLRFSTPKTPKPLLIITPTNIS 92
           E FL+C S + P++ +  K +YT  +  Y SVLN T++NLRF++  TPKPL+I+TP+N+S
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 93  HIQAAVVCSKSHGLQIRTRSGGHDFEGLSYVAHLPFIILDLIKLNSISIDVQSKTAWVQS 152
           HIQA+++CSK  GLQIRTRSGGHD EGLSY++ +PF I+DL  ++++ +D+ S+TAWV++
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +   +FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPETVTVCFANRTSE-DGATN 272
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T+    +  E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVANKLDENLFLGILLGGGNFTRQEGKINPL--ALFFSQYLGKADEVVAILKAT 332
           L ++WQ +A K D++L L       N T   GK        F S +LG  D +V ++  +
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331

Query: 333 FPELALTKKDCIEMSWIESALIMGTGLQPGKV---LEDLLSKTTITNGSTKIKSDYVKEP 392
           FPEL + K DC E+SWI++ +     +         E LL ++     +  IK DYVK+ 
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391

Query: 393 IPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQISESETPFSHRAGYMYKIGYVLTWAE 452
           IP  A+  I E+L  E++ +   +  PYGG M +ISES  PF HRAG MY++ Y  TW +
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451

Query: 453 RGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYKQASVWGLK 512
           +     ++H+NW+R +Y    P+VS++PR AY+NYRDLD+G  N     +Y QA +WG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEK 511

Query: 513 YFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 541
           YFG+ NFNRLV VKTK DP++FFR+EQSIP L P
Sbjct: 512 YFGK-NFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of Lcy06g010180 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 255/512 (49.80%), Postives = 348/512 (67.97%), Query Frame = 0

Query: 33  EGFLQCLSHHSPDSYSISKVVYTPFNSSYSSVLNFTLRNLRFSTPKTPKPLLIITPTNIS 92
           E FL+C S + P + + +K+VYT  +  Y S+LN T++NLRF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRTRSGGHDFEGLSYVAHLPFIILDLIKLNSISIDVQSKTAWVQS 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+I+DL  ++S+ IDV S+TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPETVTVCFANRTSE-DGATN 272
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP   T+    +  E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIHRWQYVANKLDENLFLGILLGGGNFTRQEGKINPL--ALFFSQYLGKADEVVAILKAT 332
           L+++WQ +A   ++ L L       N T  +GK      + F S + G  D +V ++  +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELALTKKDCIEMSWIESALIMGTGLQPGKVL---EDLLSKTTITNGSTKIKSDYVKEP 392
           FPEL + K DC ++SWI++ +     +         E LL ++     +  IK DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 IPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQISESETPFSHRAGYMYKIGYVLTWAE 452
           IP  A+  I E+L  ED+ +   +F PYGG M +ISES  PF HRAG MY+I Y+ +W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 RGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNYRDLDIGSNNKYGKTSYKQASVWGLK 512
           +     ++H+NWIR +Y    P+VS++PR AY+NYRDLD+G  N     +Y QA +WG K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTL 539
           YFG+ NFNRLV VKTK DP +FFR+EQSIP L
Sbjct: 512 YFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Lcy06g010180 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.9e-146
Identity = 258/536 (48.13%), Postives = 367/536 (68.47%), Query Frame = 0

Query: 6   LIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS--YSS 65
           L+ L L  + LSSS+L           + F++CL  +S  S+ I+   ++P  ++  +  
Sbjct: 14  LVTLLLISVPLSSSTL----------QQDFVKCLVDNSDVSFPITASFFSPDQNATLFKE 73

Query: 66  VLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGLSYV 125
            L  T +NLR+ TP  PKP+ I  P   +H+QAAVVC+K   L +R RSGGHD+EGLS+V
Sbjct: 74  ELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFV 133

Query: 126 A--HLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTVG 185
           A    PF+I+DL KL  + +D+ S +AW  +GAT+GE+YYRI EKS+T  FPAGLC ++G
Sbjct: 134 AEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLG 193

Query: 186 VGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 245
           +GGH  GG YG M+RK+GL ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+
Sbjct: 194 IGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGV 253

Query: 246 VVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTRQ 305
           ++AWK+KLVPVP TVTV    +T E   T ++++W+ +A+KLD++LF+ +++   + T +
Sbjct: 254 ILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTK 313

Query: 306 EGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGKV 365
            G       + +Q+LG ++ ++ +++ +FPEL LTKKDC EMSWI+S + +  G      
Sbjct: 314 PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI-AGFPNSAA 373

Query: 366 LEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQI 425
            E LL+  ++     K KSD+VKEPIP+  +EG+WER   ED   P  I+ PYGG MS+I
Sbjct: 374 PEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRI 433

Query: 426 SESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNY 485
           SESE PF HR G ++KI ++ TW + G  +E+RH+ WIRE+Y +M  +VSK+PR AYVNY
Sbjct: 434 SESEIPFPHRNGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNY 493

Query: 486 RDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPT 538
           RDLD+G+N   G+T  ++   WG KY+ + NF RLV +K + DP +FFRHEQS+PT
Sbjct: 494 RDLDLGTNE--GETDARE---WGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Lcy06g010180 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.4e-260
Identity = 434/541 (80.22%), Postives = 486/541 (89.83%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYYRIA+KSRTLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q GKINPLALFFS YLGKADE++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSK  + NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M Q
Sbjct: 366 KLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           I E ETPFSHR  Y+YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.2e-258
Identity = 430/541 (79.48%), Postives = 485/541 (89.65%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV++KTAWVQSGATLGELYY IA+KS+TLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q GKINPLALFFS YLGKA+E++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSK T+ NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           I E ETPFSHR   +YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.9e-257
Identity = 434/545 (79.63%), Postives = 484/545 (88.81%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V+AN+G+ HE FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YS+VLNFTLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYY IA+KS+TL FPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYG +LRKYGLAADNVIDA LVDANGKF +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLV VP +VT+C  N+TSEDGAT LIH+WQYVA+KL+ ++FLGILLGGGNFTR
Sbjct: 246 IVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGT----GL 360
           Q  KINP+ALFFS YLGKADE+VAIL  TFPEL L+KKDC+E SW+E+A+IMGT    GL
Sbjct: 306 QGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGL 365

Query: 361 QPGKVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGG 420
           QPG  LEDLLSKTT    STKIKSDYVKEPIP+AAIEGIWERL+ +DIE+PQ IFVPYGG
Sbjct: 366 QPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGG 425

Query: 421 RMSQISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRA 480
           RM QISESETPFSHR  YMYKIGYV+ W E+G+ AEKRH++WIRELY +M PFVSKSPRA
Sbjct: 426 RMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRA 485

Query: 481 AYVNYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIP 540
           AY+NYRDLDIGSNNKYG+TSY+QAS+WG KYFG  NF+RLVHVKTK DP+DFFRHEQSIP
Sbjct: 486 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHEQSIP 545

Query: 541 TLSPK 542
           TLS K
Sbjct: 546 TLSSK 549

BLAST of Lcy06g010180 vs. ExPASy TrEMBL
Match: A0A6J1GRE5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456787 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.1e-253
Identity = 426/541 (78.74%), Postives = 475/541 (87.80%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + HE FLQCLSH+S D+ SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YS VLNFTLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHD EGL
Sbjct: 66  YSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYYRIAEKSR+LAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG++LRKYGLAADNVIDA LVDANGKF DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKV LV VP TVT+C  N+T EDGAT LIH+WQYVA+KL+ NLFLGILL GGNFTR
Sbjct: 246 IVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q  KINPLALFFS YLGKADE++ IL  TFPEL L+KKDCIE SW+E+ALIMGTGLQPG 
Sbjct: 306 QGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSKT +   STK+KSDYVKEPIP+AAIEGI +RL+  DIE PQ+IF+PYGG+MS+
Sbjct: 366 KLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSE 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           ISE ETPFSHR  Y YKIGY  +W ERG+ AE +HLNWIRELY +MAPFVSKSPRAAY+N
Sbjct: 426 ISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QA +WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPT S 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTFSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. ExPASy TrEMBL
Match: A0A6J1K3Z1 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490441 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.4e-252
Identity = 426/545 (78.17%), Postives = 480/545 (88.07%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLIKLNSISIDV+SKTAWVQSGATLGELYY IA+KS+TLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYG +LRKYGLAADNVIDA LVDANGKF +RE MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKV LV VP +VT+C A++TSEDGAT LIH+WQYVA+KL+ ++FLGILLGGGNF+R
Sbjct: 246 IVVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFSR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGT----GL 360
           Q  KINP+ALFFS YLGKADE+VAIL   FPEL L+KKDC+E SW+E+A+IMGT    GL
Sbjct: 306 QGDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGLQPGL 365

Query: 361 QPGKVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGG 420
           QPG  LEDL SKTT  N STKIKSDYVKEPIP+AAIEGIWERL+ +DIE+PQ +FVPYGG
Sbjct: 366 QPGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFVPYGG 425

Query: 421 RMSQISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRA 480
           RM QI E ETPFSHR  YMYKIGY + W E G+ AEKRH++WIRE+Y +M PFVSKSPRA
Sbjct: 426 RMGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSKSPRA 485

Query: 481 AYVNYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIP 540
           AY+NYRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIP
Sbjct: 486 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 545

Query: 541 TLSPK 542
           TLS K
Sbjct: 546 TLSSK 549

BLAST of Lcy06g010180 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 907.1 bits (2343), Expect = 7.1e-260
Identity = 434/541 (80.22%), Postives = 486/541 (89.83%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYYRIA+KSRTLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q GKINPLALFFS YLGKADE++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSK  + NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M Q
Sbjct: 366 KLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           I E ETPFSHR  Y+YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. NCBI nr
Match: XP_038895570.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 906.4 bits (2341), Expect = 1.2e-259
Identity = 439/542 (81.00%), Postives = 488/542 (90.04%), Query Frame = 0

Query: 1   MKSSPLI-PLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNS 60
           MKSSPLI  LSL  I+LSSS+ W VA+N  EKHE FL+CL HHSP   +ISKV+YTPFNS
Sbjct: 1   MKSSPLILLLSLVVILLSSSAPW-VASNSSEKHENFLECLFHHSP---NISKVIYTPFNS 60

Query: 61  SYSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEG 120
           SYSS+LNFTLRNLRFSTP TPKPLLIITP++ISHIQ AV+CSKS+GLQIRTRSGGHDFEG
Sbjct: 61  SYSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEG 120

Query: 121 LSYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPT 180
           LSYVA+LPFII+DLI L+SI I++++ TAWV+SGATLGELYYRIAEKSRTLAFPAG CP 
Sbjct: 121 LSYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPI 180

Query: 181 VGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGH SGGGYGM++RKYGLAADNVIDAYLVDANG+FHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFT 300
           GIVVAWKVKLV VP TVT+CF ++T E+GATNLIHRWQYVA+ LDENLF+GILL GGNFT
Sbjct: 241 GIVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFT 300

Query: 301 RQEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPG 360
           RQEGK NP ALFFS YLGK DE+V+IL  TFPEL L KKDCIE SW+ESALIMGTGLQPG
Sbjct: 301 RQEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPG 360

Query: 361 KVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMS 420
           K LEDLLSK ++TNG+TKIKSDY+K+PIPLAA+EGIW+RLK EDIEMPQL+F PYGGRMS
Sbjct: 361 KSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMS 420

Query: 421 QISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYV 480
           QISESE+PFSHRAGY+YKIGYV  W ++    EKRHL+WIRELY +M PFVSKSPRAAY+
Sbjct: 421 QISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYI 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLS 540
           NYRDLDIGSNNKYGKTSYKQAS+WGLKYFG NNFNRLV VKT+ DPH+FFRHEQSIPT S
Sbjct: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFS 537

Query: 541 PK 542
            K
Sbjct: 541 NK 537

BLAST of Lcy06g010180 vs. NCBI nr
Match: KAG6573426.1 (Cannabidiolic acid synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 903.3 bits (2333), Expect = 1.0e-258
Identity = 432/541 (79.85%), Postives = 485/541 (89.65%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SS L+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNS+
Sbjct: 6   MNSSSLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNST 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYY IA+KS+TLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q GKINPLALFFS YLGKADE++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSK T+ NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           I E ETPFSHR  Y+YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. NCBI nr
Match: XP_023542525.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 434/542 (80.07%), Postives = 487/542 (89.85%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V+AN+ + H+ FLQCLSH+S D+ SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSANFVKPHQSFLQCLSHYSQDASSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV+SKTAWVQSGATLGELYY IA+KS+TLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRES-MGEDLFWAIRGGGGGSF 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF +R+  MGEDLFWAIRGGGGGSF
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKKLMGEDLFWAIRGGGGGSF 245

Query: 241 GIVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFT 300
           GIVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFT
Sbjct: 246 GIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFT 305

Query: 301 RQEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPG 360
           RQ GKINPLALFFS YLGKADE++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G
Sbjct: 306 RQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQFG 365

Query: 361 KVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMS 420
             LEDLLSK T+ NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M 
Sbjct: 366 SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMG 425

Query: 421 QISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYV 480
           QI E ETPFSHR  Y+YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+
Sbjct: 426 QIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYL 485

Query: 481 NYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLS 540
           NYRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS
Sbjct: 486 NYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLS 545

Query: 541 PK 542
            K
Sbjct: 546 SK 546

BLAST of Lcy06g010180 vs. NCBI nr
Match: XP_022954543.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 430/541 (79.48%), Postives = 485/541 (89.65%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS 60
           M SSPL+PLSL  +VL S S W V++N+ + H+ FLQCLSH+S D  SIS V+YTPFNSS
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSW-VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGL 120
           YSSVLN TLRNLRFSTP TPKPLLIITP ++SHIQAAV+CSKSHGLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTV 180
           SYVA LPFII+DLI LNSISIDV++KTAWVQSGATLGELYY IA+KS+TLAFPAG+CPTV
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGM+LRKYGLAADNVIDA LVDANGKF +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 300
           IVVAWKVKLVPVP +VT+C AN+TSEDGAT LIH+WQYVA+KL+ N FLGILL GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGK 360
           Q GKINPLALFFS YLGKA+E++ IL  TFPEL L+KKDC+E SW+E+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 VLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQ 420
            LEDLLSK T+ NGSTKIKSDY+KEPIP+AAIEGIWERL++EDIEMPQ IFVPYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVN 480
           I E ETPFSHR   +YKIGY++ WAE+G+ AEKRH++WIRELY +M PFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLSP 540
           YRDLDIGSNNKYG+TSY+QAS+WG KYFG+ NF+RLVHVKTK DP+DFFRHEQSIPTLS 
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

Query: 541 K 542
           K
Sbjct: 546 K 545

BLAST of Lcy06g010180 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 6.4e-150
Identity = 272/539 (50.46%), Postives = 365/539 (67.72%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFIVLSSSSLWI---VAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPF 60
           MK        L F   S+  L I   V+AN   +   FLQCLS    DS  +SKV++TP 
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQ-SSFLQCLSLQLNDSNIVSKVIHTPN 60

Query: 61  NSSYSSVLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDF 120
           ++S+SSVL  +++N RFS P  PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+
Sbjct: 61  DTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 120

Query: 121 EGLSYVAHLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLC 180
           EGLSYV H PF+ILDL  L SI++DV +++ WVQ+GAT+GELYY I +K+RTLAFPAG+C
Sbjct: 121 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 180

Query: 181 PTVGVGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGG 240
           PTVGVGGHFSGGGYG +LRK+GLAAD+VIDA +VDA G+  +R  MGED FWAIRGGGG 
Sbjct: 181 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 240

Query: 241 SFGIVVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGN 300
           SF +V++WK+ L+ VP TVTV    + SE  A  +IHRWQ+VA+K+ ++LF+ ++L    
Sbjct: 241 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML---- 300

Query: 301 FTRQEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQ 360
              Q  K    A F   YLG    ++ ++   FPEL L + DC EMSWIES +      +
Sbjct: 301 ---QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---E 360

Query: 361 PGKVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGR 420
            G+   ++L+K T  + + K KSD+V+EP+P  AI  +W RL+  + E  QLIF P+GG+
Sbjct: 361 LGEEPINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 420

Query: 421 MSQISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAA 480
           MS+I++ ETPF HR G +Y+I Y+  W  RG   EK ++ W+  +Y  M+ FV+KSPR A
Sbjct: 421 MSEIADYETPFPHRKGNIYEIQYLNYW--RGDVKEK-YMRWVERVYDDMSEFVAKSPRGA 480

Query: 481 YVNYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIP 537
           Y+N RDLD+G      ++ Y++   WG+KYF +NNF RLV VKT  DP DFF  EQSIP
Sbjct: 481 YINLRDLDLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Lcy06g010180 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.3e-147
Identity = 258/536 (48.13%), Postives = 367/536 (68.47%), Query Frame = 0

Query: 6   LIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSS--YSS 65
           L+ L L  + LSSS+L           + F++CL  +S  S+ I+   ++P  ++  +  
Sbjct: 14  LVTLLLISVPLSSSTL----------QQDFVKCLVDNSDVSFPITASFFSPDQNATLFKE 73

Query: 66  VLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGLSYV 125
            L  T +NLR+ TP  PKP+ I  P   +H+QAAVVC+K   L +R RSGGHD+EGLS+V
Sbjct: 74  ELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFV 133

Query: 126 A--HLPFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTVG 185
           A    PF+I+DL KL  + +D+ S +AW  +GAT+GE+YYRI EKS+T  FPAGLC ++G
Sbjct: 134 AEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLG 193

Query: 186 VGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 245
           +GGH  GG YG M+RK+GL ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+
Sbjct: 194 IGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGV 253

Query: 246 VVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTRQ 305
           ++AWK+KLVPVP TVTV    +T E   T ++++W+ +A+KLD++LF+ +++   + T +
Sbjct: 254 ILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTK 313

Query: 306 EGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGKV 365
            G       + +Q+LG ++ ++ +++ +FPEL LTKKDC EMSWI+S + +  G      
Sbjct: 314 PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI-AGFPNSAA 373

Query: 366 LEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQI 425
            E LL+  ++     K KSD+VKEPIP+  +EG+WER   ED   P  I+ PYGG MS+I
Sbjct: 374 PEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRI 433

Query: 426 SESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNY 485
           SESE PF HR G ++KI ++ TW + G  +E+RH+ WIRE+Y +M  +VSK+PR AYVNY
Sbjct: 434 SESEIPFPHRNGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNY 493

Query: 486 RDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPT 538
           RDLD+G+N   G+T  ++   WG KY+ + NF RLV +K + DP +FFRHEQS+PT
Sbjct: 494 RDLDLGTNE--GETDARE---WGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Lcy06g010180 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 267/540 (49.44%), Postives = 361/540 (66.85%), Query Frame = 0

Query: 8   PLSLAFIVLSSSSLWIV--AANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFN--SSYSS 67
           PL L  I++   SL+ +   ++     + F+ C+  ++   + + K  + P    S +S 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQ 65

Query: 68  VLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGLSYV 127
           VL  T +NLRF     PKP  I +P + SH+QA+++CSK   + +R RSGGHD+EGLSYV
Sbjct: 66  VLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYV 125

Query: 128 AHL--PFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTVG 187
           + +  PFI++DL K+  ++I++Q  +AWVQSGAT+GELYYRIAEKS+   FPAGLC ++G
Sbjct: 126 SQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLG 185

Query: 188 VGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 247
           +GGH +GG YG M+RKYGL ADNV+DA +VDANGK  DR +MGED FWAIRGG GGSFGI
Sbjct: 186 IGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGI 245

Query: 248 VVAWKVKLVPVPETVTVCFANRT-SEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTR 307
           ++AWK+KLVPVP+TVTV    +T  +D    +I +WQ VA+KL E LF+ +L    N   
Sbjct: 246 ILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAG 305

Query: 308 QEGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIES-ALIMGTGLQ-P 367
             G       + + +LG    ++ ++K +FPEL LT KDCIEMSW+ES A I G     P
Sbjct: 306 TGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTP 365

Query: 368 GKVLEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRM 427
             VL  L  K+     S K KSD+VK PIP + ++GI+++L  EDI  P +I+ PYGG M
Sbjct: 366 TNVL--LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMM 425

Query: 428 SQISESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAY 487
           ++I ES+ PF HR G ++K+ YV +W +       RH+NWIR+LY +M P+VS +PR AY
Sbjct: 426 AKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPREAY 485

Query: 488 VNYRDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTL 539
           VNYRDLD+G N K  KT  KQA VWG  YF +NNFNRL+ +K K DP +FFRHEQSIP +
Sbjct: 486 VNYRDLDLGRNTKDVKTCIKQAQVWGANYF-KNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lcy06g010180 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 514.6 bits (1324), Expect = 9.6e-146
Identity = 257/530 (48.49%), Postives = 362/530 (68.30%), Query Frame = 0

Query: 14  IVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYTPFNSSYSSVLNFTLRNLR 73
           I+L S ++  + + +G  HE FL+CLS+   D+    KV++T  +SS+ S+L+ +++N R
Sbjct: 10  ILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPR 69

Query: 74  FSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGLSYVAH-LPFIILD 133
           FS  +TPKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGLSY+A+  PF ++D
Sbjct: 70  FSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVID 129

Query: 134 LIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTVGVGGHFSGGGYG 193
           L  L SIS+DV ++T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG FSGGGYG
Sbjct: 130 LRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYG 189

Query: 194 MMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 253
            +LRKYGLAADN+IDA +VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV V
Sbjct: 190 TLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDV 249

Query: 254 PETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTRQEGKINPLALFF 313
           P T+TV    +TS+  A  +I +WQY A+K+ ++LF+   L   N      K    ALF 
Sbjct: 250 PSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN------KNAVHALFT 309

Query: 314 SQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGKVLEDLLSKTTIT 373
             Y+G  + ++A+++  FPEL L K+ C EMSWIES L        G+ L  +L+    T
Sbjct: 310 GLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWF-ADFPKGESL-GVLTNRERT 369

Query: 374 NGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQISESETPFSHRA 433
           + S K K D+V+EPIP AAI+ IW RL+  +  + ++I  P+GG+MS+++E ETPF HR 
Sbjct: 370 SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRG 429

Query: 434 GYMYKIGYVLTWAE---RGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNYRDLDIGSN 493
           G +Y+I YV  W E   +      ++L W+  +Y  M P+VSKSPR AYVN++D+D+G  
Sbjct: 430 GNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMY 489

Query: 494 NKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPTLS 540
               KT Y++   WG+KYF +NNF RLV VKT+ DP DFF  EQSIP ++
Sbjct: 490 LGKKKTKYEEGKSWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Lcy06g010180 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 508.8 bits (1309), Expect = 5.2e-144
Identity = 257/536 (47.95%), Postives = 362/536 (67.54%), Query Frame = 0

Query: 6   LIPLSLAFIVLSSSSLWIVAANYGEKHEGFLQCLSHHSPDSYSISKVVYT-PFN-SSYSS 65
           L+ LSL++I LS S++           + F+ CL  +S  S+ +    +T   N SS+  
Sbjct: 16  LLLLSLSYIPLSFSTI----------QQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKL 75

Query: 66  VLNFTLRNLRFSTPKTPKPLLIITPTNISHIQAAVVCSKSHGLQIRTRSGGHDFEGLSYV 125
            L  + +NLR+  P  PKP  I  P   +H+QAAV+C+K   L +R RSGGHD+EGLSYV
Sbjct: 76  ALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYV 135

Query: 126 AHL--PFIILDLIKLNSISIDVQSKTAWVQSGATLGELYYRIAEKSRTLAFPAGLCPTVG 185
           + +   F+I+DL KL  IS+D++S +AWV +GA++GE+YYRI EKS+   FPAGLC ++G
Sbjct: 136 SEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLG 195

Query: 186 VGGHFSGGGYGMMLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 245
           +GGH  GG YG M+RK+GL ADNV+DA +VDA+GK  +R +MGED+FWAIRGGGGGSFG+
Sbjct: 196 IGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGV 255

Query: 246 VVAWKVKLVPVPETVTVCFANRTSEDGATNLIHRWQYVANKLDENLFLGILLGGGNFTRQ 305
           ++AWK+KLVPVPE VTV    RT E   T L+++WQ VA+KLDE+LF+ +++   + T +
Sbjct: 256 ILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPK 315

Query: 306 EGKINPLALFFSQYLGKADEVVAILKATFPELALTKKDCIEMSWIESALIMGTGLQPGKV 365
             +      +  Q+LG A+ ++ +++ +FP+L LTKKDC+E SWI+S + +  G      
Sbjct: 316 SKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYI-AGFPSTAP 375

Query: 366 LEDLLSKTTITNGSTKIKSDYVKEPIPLAAIEGIWERLKFEDIEMPQLIFVPYGGRMSQI 425
            E LL   ++     K KSDYV+EPIP+  +EG+WE+L  ED   P  I+ PYGG M++I
Sbjct: 376 SEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGMMAKI 435

Query: 426 SESETPFSHRAGYMYKIGYVLTWAERGVAAEKRHLNWIRELYGHMAPFVSKSPRAAYVNY 485
            E+ETPF HR+G ++KI ++  W + G  +E +H+ W+RE+Y +M  +VSKSPR+AYVNY
Sbjct: 436 PETETPFPHRSGTLFKIQWLTLWQD-GKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNY 495

Query: 486 RDLDIGSNNKYGKTSYKQASVWGLKYFGRNNFNRLVHVKTKTDPHDFFRHEQSIPT 538
           RDLD+G N K        A  WG +YF + NF RLV +K K DP +FFRHEQSIPT
Sbjct: 496 RDLDLGMNGK-----GSDAREWGNRYF-KGNFERLVEIKAKFDPENFFRHEQSIPT 531