Lcy06g007200 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy06g007200
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	lactosylceramide 4-alpha-galactosyltransferase-like
Location	Chr06: 7026047 .. 7027886 (-)
RNA-Seq Expression	Lcy06g007200
Synteny	Lcy06g007200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCTCATTTACACTCCGATTGATTTTCATTACGATTACAATCTATTTCCATCGAGGTAAGTAAACAAAACCTAAATACAATATTTTGTAGAGTAGTATTCTAATCTTTTTTTTTTTTCAAAAACCCAACCCAATATTTAACATCCACGTATTCAAATAGCAACACCCAATATGCCAAGGGAAAGAAAAAAGAAACCAAAGCTGGCCATTGGGCAGTGACAATTCCATCCTCTCCTTTTCACAACTTTTTTGGTTTTCTCACAATCACATGCACATTCCCCAATTCTCTTTTCAAAACCCACCTTTCTATTTCATCTTTTCATTTCATTTATTTTCCTCCTCAGTCCACCATTAAAATGCTCTGCAACTTTCTCGAGCTCCAATGCTCTCACTCACTCCTATTTCTTCTCCTCCTCCATTTGTCAGTACCTTTCACTTTCAAAAACTCCAGAACTTTCCTCAGAATATTCCTCTTCCTCCATTTCCAATGATTGCTAGTTTCAACCTTCACCGTTTACAAACTCTCAAGGAATCCACTTTCGATCTTCTCTCTTCCCTTCCCACTTCTCTTCTTTCTCTTCTCCTTCTCTTCCTCTTGGCTTACAATGCCCTTTACGTTTTCAGCATTTTCCCACCGTCGCCGGAGAAGACGATTCCACAAGCTGCCGGTTTCTCGCCGGAGAAACGATGGTCCAATCTTTCTTCTTCAGTTCCCACTTCGACCCATGTTTCCTATTCTATAGAAGAAAAAAACCCACCGGCGATTTTGAAATCAAATTCCTCTGTTTTTCACCATCCCCACTTCAAAATCCGCCGCGCCGGTAACCCCGCCGGCCGGCCGACGAAGAGGGCCGGAAAGCACAAACGCCGCCTCAGAAGCCTCCGGCCGGAATCAAGAGAAAACGAATTCTCAGCAAGAATCGAAGAGTTTTTTGCTGCGAATTCGTGTAAGCTCAGATTCTTCATGACTTGGATTTCACCATTGAATTCGTTTAGCGACAGAGAATTATGGGCAATACAGAGCATATTCAAAGCCCATGAAAATGAAACTCCAAATGAAAATGGAAATGGAAATCCCTGTTTGGTCATCGTCTCGAATTCCCTCGATTCCACGAAAGGGAAACAAATTCTCACTCCAATCTCGAAAAAGGGATTCCCCATAATCGCAATCTCGCCGAACTTCGATTCAATTTTCAGGAACACCGAAGCAGAGCCATGGTTCAACCAACTCCGGCAAGGAATCATAAAACCCGGCGAGATTTCCTTAGGCCAAAACCTCTCAAACTTGCTCAGATTGGCTCTGCTATACAAATTCGGCGGCATTTACATCGACACCGACGTGATAATTCTAAAGAGCTTCACAAATCTCCGCAACGTGATCGGAGCTCAAACCATCGATTTGAAAACAGGGAACTGGAGCAGATTGAACAACGCAGTGATGATTTTCGACAAGAATCACCCACTTCTTCTTCAGTTCATCCAAGAATTCGCCTCGACATTCGACGGAAACAAATGGGGTCACAACGGACCGTACTTAGTTTCAAGAGTTGTATCGAGATTGAACGAAAATCCTGGGTTTAATTTCACTGTTCTTCCTCCGTCGGCGTTTTACCCTGTGGTTTGGAGCAGAATCAGAACTCTGTTTCAAGGTCCAAAAGATGCAGTTCATTTGAAATGGCTGATTGCGAAATTGAGACAAATTCGATCCAACAGTTTGGCTCTGCATTTGTGGAACAGCCATAGCCGAAAGCTTGAAGTCGAAAAGGGCAGCATTGTTGATGTAATAAGATGGAGTTCGTGTGTTTTCTGCAATTACTCTGCTTCTGGATTACGATAA

mRNA sequence

ATGAGCCTCATTTACACTCCGATTGATTTTCATTACGATTACAATCTATTTCCATCGAGAGAATTATGGGCAATACAGAGCATATTCAAAGCCCATGAAAATGAAACTCCAAATGAAAATGGAAATGGAAATCCCTGTTTGGTCATCGTCTCGAATTCCCTCGATTCCACGAAAGGGAAACAAATTCTCACTCCAATCTCGAAAAAGGGATTCCCCATAATCGCAATCTCGCCGAACTTCGATTCAATTTTCAGGAACACCGAAGCAGAGCCATGGTTCAACCAACTCCGGCAAGGAATCATAAAACCCGGCGAGATTTCCTTAGGCCAAAACCTCTCAAACTTGCTCAGATTGGCTCTGCTATACAAATTCGGCGGCATTTACATCGACACCGACGTGATAATTCTAAAGAGCTTCACAAATCTCCGCAACGTGATCGGAGCTCAAACCATCGATTTGAAAACAGGGAACTGGAGCAGATTGAACAACGCAGTGATGATTTTCGACAAGAATCACCCACTTCTTCTTCAGTTCATCCAAGAATTCGCCTCGACATTCGACGGAAACAAATGGGGTCACAACGGACCGTACTTAGTTTCAAGAGTTGTATCGAGATTGAACGAAAATCCTGGGTTTAATTTCACTGTTCTTCCTCCGTCGGCGTTTTACCCTGTGGTTTGGAGCAGAATCAGAACTCTGTTTCAAGGTCCAAAAGATGCAGTTCATTTGAAATGGCTGATTGCGAAATTGAGACAAATTCGATCCAACAGTTTGGCTCTGCATTTGTGGAACAGCCATAGCCGAAAGCTTGAAGTCGAAAAGGGCAGCATTGTTGATGTAATAAGATGGAGTTCGTGTGTTTTCTGCAATTACTCTGCTTCTGGATTACGATAA

Coding sequence (CDS)

ATGAGCCTCATTTACACTCCGATTGATTTTCATTACGATTACAATCTATTTCCATCGAGAGAATTATGGGCAATACAGAGCATATTCAAAGCCCATGAAAATGAAACTCCAAATGAAAATGGAAATGGAAATCCCTGTTTGGTCATCGTCTCGAATTCCCTCGATTCCACGAAAGGGAAACAAATTCTCACTCCAATCTCGAAAAAGGGATTCCCCATAATCGCAATCTCGCCGAACTTCGATTCAATTTTCAGGAACACCGAAGCAGAGCCATGGTTCAACCAACTCCGGCAAGGAATCATAAAACCCGGCGAGATTTCCTTAGGCCAAAACCTCTCAAACTTGCTCAGATTGGCTCTGCTATACAAATTCGGCGGCATTTACATCGACACCGACGTGATAATTCTAAAGAGCTTCACAAATCTCCGCAACGTGATCGGAGCTCAAACCATCGATTTGAAAACAGGGAACTGGAGCAGATTGAACAACGCAGTGATGATTTTCGACAAGAATCACCCACTTCTTCTTCAGTTCATCCAAGAATTCGCCTCGACATTCGACGGAAACAAATGGGGTCACAACGGACCGTACTTAGTTTCAAGAGTTGTATCGAGATTGAACGAAAATCCTGGGTTTAATTTCACTGTTCTTCCTCCGTCGGCGTTTTACCCTGTGGTTTGGAGCAGAATCAGAACTCTGTTTCAAGGTCCAAAAGATGCAGTTCATTTGAAATGGCTGATTGCGAAATTGAGACAAATTCGATCCAACAGTTTGGCTCTGCATTTGTGGAACAGCCATAGCCGAAAGCTTGAAGTCGAAAAGGGCAGCATTGTTGATGTAATAAGATGGAGTTCGTGTGTTTTCTGCAATTACTCTGCTTCTGGATTACGATAA

Protein sequence

MSLIYTPIDFHYDYNLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRSNSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCNYSASGLR

Homology

[Close]

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot
Match: Q67BJ4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4galt PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-24
Identity = 67/214 (31.31%), Postives = 110/214 (51.40%), Query Frame = 0


Query: 71  FPIIAISP-NFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYI 130
           FP + I P +   +F +T    W+++ R    +P ++ +   LS+  R+ALL+KFGGIY+
Sbjct: 142 FPNVWIRPLDLQELFEDTPLAAWYSEARHR-WEPYQLPV---LSDASRIALLWKFGGIYL 201

Query: 131 DTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGN 190
           DTD I+LK+  NL N +G Q+  +       LN A + F++ H  L   + +F + ++G 
Sbjct: 202 DTDFIVLKNLLNLTNTLGIQSRYV-------LNGAFLAFERKHEFLALCLHDFVANYNGW 261

Query: 191 KWGHNGPYLVSRVVSRL-------NENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVH 250
            WGH GP L++RV  +          +     T LPP AFYP+ W   +  F+       
Sbjct: 262 IWGHQGPQLLTRVFKKWCSIQSLEKSHACRGVTALPPEAFYPIPWQNWKKYFEDISP--- 321

Query: 251 LKWLIAKLRQIRSNSLALHLWNSHSRKLEVEKGS 277
                 +L Q+ + + A+H+WN  S+   +E  S
Sbjct: 322 -----EELTQLLNATYAVHVWNKKSQGTHLEATS 336

[Close]

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot
Match: Q9JI93 (Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=A4galt PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.9e-24
Identity = 70/214 (32.71%), Postives = 110/214 (51.40%), Query Frame = 0


Query: 71  FPIIAISP-NFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYI 130
           FP + I P +   +F +T    W+ + +       E  L   LS+  R+ALL+KFGGIY+
Sbjct: 143 FPNVQIRPLDLQELFEDTPLAAWYLEAQH----RWEPYLLPVLSDASRIALLWKFGGIYL 202

Query: 131 DTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGN 190
           DTD I+LK+  NL N++G Q+  +       LN A + F++ H  L   I++F + ++G 
Sbjct: 203 DTDFIVLKNLRNLTNMLGIQSRYV-------LNGAFLAFERKHEFLALCIRDFVAHYNGW 262

Query: 191 KWGHNGPYLVSRV------VSRLNENPGF-NFTVLPPSAFYPVVWSRIRTLFQGPKDAVH 250
            WGH GP L++RV      +  L E+      T LPP AFYP+ W   +  F+       
Sbjct: 263 IWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSP--- 322

Query: 251 LKWLIAKLRQIRSNSLALHLWNSHSRKLEVEKGS 277
                 +L Q+ + + A+H+WN  S+   +E  S
Sbjct: 323 -----EELAQLLNATYAVHVWNKKSQGTHLEATS 337

[Close]

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot
Match: P0C8Q4 (Uncharacterized protein At4g19900 OS=Arabidopsis thaliana OX=3702 GN=At4g19900 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.0e-24
Identity = 83/284 (29.23%), Postives = 130/284 (45.77%), Query Frame = 0


Query: 16  LFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIA 75
           +F  R    ++S+   H           + C+V+ S +++    +       K  + +  
Sbjct: 382 MFSVRHQRGLESLLSQHR----------DACVVVFSETVELDFFRNSFV---KDSYKVAV 441

Query: 76  ISPNFDSIFRNTE----AEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDT 135
             PN D + ++T     A  WF+  R+    P       + S L+RLA LYK+GG+Y+D+
Sbjct: 442 AMPNLDELLQDTPTHVFASVWFD-WRKTKFYP------THYSELVRLAALYKYGGVYLDS 501

Query: 136 DVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKW 195
           DVI+L S ++LRN IG +  D   G    LN AVM F+K  P LL+ + E+  T+D    
Sbjct: 502 DVIVLGSLSSLRNTIGME--DQVAG--ESLNGAVMSFEKKSPFLLECLNEYYLTYDDKCL 561

Query: 196 GHNGPYLVSRVVSRL--NENPGFN---FTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWL 255
             NG  L++RV  R    +N   N     + P S F+P+   +I   F  P         
Sbjct: 562 RCNGADLLTRVAKRFLNGKNRRMNQQELNIRPSSVFFPINSQQITNYFAYPAIEDERSQQ 621

Query: 256 IAKLRQIRSNSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCN 291
               ++I + SL  H WNS +  L  E  S+V      SC+ C+
Sbjct: 622 DESFKKILNESLTFHFWNSVTSSLIPEPESLVAKFLDHSCIRCS 641

[Close]

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot
Match: Q9NPC4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.2e-23
Identity = 69/214 (32.24%), Postives = 112/214 (52.34%), Query Frame = 0


Query: 71  FPIIAISP-NFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYI 130
           FP + + P +   +FR+T    W+  + QG  +P  + +   LS+  R+AL++KFGGIY+
Sbjct: 136 FPNVQMLPLDLRELFRDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYL 195

Query: 131 DTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGN 190
           DTD I+LK+  NL NV+G Q+  +       LN A + F++ H  +   +++F   ++G 
Sbjct: 196 DTDFIVLKNLRNLTNVLGTQSRYV-------LNGAFLAFERRHEFMALCMRDFVDHYNGW 255

Query: 191 KWGHNGPYLVSRV------VSRLNENPGF-NFTVLPPSAFYPVVWSRIRTLFQGPKDAVH 250
            WGH GP L++RV      +  L E+      T LPP AFYP+ W   +  F+       
Sbjct: 256 IWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINP--- 315

Query: 251 LKWLIAKLRQIRSNSLALHLWNSHSRKLEVEKGS 277
                 +L ++ S + A+H+WN  S+    E  S
Sbjct: 316 -----EELPRLLSATYAVHVWNKKSQGTRFEATS 330

[Close]

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot
Match: Q9N289 (Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Pongo pygmaeus OX=9600 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.2e-23
Identity = 68/214 (31.78%), Postives = 111/214 (51.87%), Query Frame = 0


Query: 71  FPIIAISP-NFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYI 130
           FP + + P +   +FR+T    W+  + QG  +P  + +   LS+  R+AL++KFGGIY+
Sbjct: 1   FPNVQMLPLDLRELFRDTPLADWYTAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYL 60

Query: 131 DTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGN 190
           DTD I+LK+  NL NV+G Q+  +       LN A + F + H  +   +++F   ++G 
Sbjct: 61  DTDFIVLKNLRNLTNVLGTQSRYV-------LNGAFLAFQRRHEFMALCMRDFVDHYNGW 120

Query: 191 KWGHNGPYLVSRV------VSRLNENPGF-NFTVLPPSAFYPVVWSRIRTLFQGPKDAVH 250
            WGH GP L++RV      +  L E+      T LPP AFYP+ W   +  F+       
Sbjct: 121 IWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDISP--- 180

Query: 251 LKWLIAKLRQIRSNSLALHLWNSHSRKLEVEKGS 277
                 +L ++ + + A+H+WN  S+    E  S
Sbjct: 181 -----EELPRLLNATYAVHVWNKKSQGTRFEATS 195

[Close]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL
Match: A0A6J1CG17 (uncharacterized protein At4g19900 OS=Momordica charantia OX=3673 GN=LOC111010894 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.2e-135
Identity = 234/281 (83.27%), Postives = 257/281 (91.46%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RELWAIQS+FKAH        GNGN CLVIVSNSLDST+G+QIL+  S+ GF +IAI
Sbjct: 38  FSDRELWAIQSLFKAH--------GNGNSCLVIVSNSLDSTEGRQILSQFSENGFRVIAI 97

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
           SP+FDSIFR+TEAE WFNQLR+GIIKPGEI++GQNLSNLLRLALLYKFGGIYID DVIIL
Sbjct: 98  SPDFDSIFRDTEAEAWFNQLRRGIIKPGEIAIGQNLSNLLRLALLYKFGGIYIDADVIIL 157

Query: 137 KSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGP 196
           KSF NLRN IGAQT+DL+TGNWSRLNNAVMIFDKNHPLLL+FI+EFASTFDGN+WGHNGP
Sbjct: 158 KSFLNLRNAIGAQTVDLRTGNWSRLNNAVMIFDKNHPLLLEFIKEFASTFDGNRWGHNGP 217

Query: 197 YLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRSN 256
           YLVSRVVSRLN+NPGFNF+VLPPSAFYPVVWSR+R LFQGPKDAVH KW+IAKLRQIRS 
Sbjct: 218 YLVSRVVSRLNQNPGFNFSVLPPSAFYPVVWSRVRGLFQGPKDAVHSKWVIAKLRQIRSK 277

Query: 257 SLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCNYSASGLR 298
           SLALHLWNSHSRKLEVEKGSI+D+IRW+SCVFCN SAS LR
Sbjct: 278 SLALHLWNSHSRKLEVEKGSIIDLIRWNSCVFCNDSASRLR 310

[Close]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL
Match: A0A6J1EH01 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111434013 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.3e-131
Identity = 229/267 (85.77%), Postives = 243/267 (91.01%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE WAIQSIFKAHE        NGNPCL IVSNSLDSTKGKQIL+P SKKGFP+I
Sbjct: 162 NSFSDREFWAIQSIFKAHE--------NGNPCLTIVSNSLDSTKGKQILSPFSKKGFPLI 221

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           AISP+FDSIFRNT+AEPWFNQLRQG IKPGEISL QNLSNLLRLALLYKFGGIY+D DVI
Sbjct: 222 AISPDFDSIFRNTQAEPWFNQLRQGTIKPGEISLAQNLSNLLRLALLYKFGGIYLDADVI 281

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFA+TFDGNKWGHN
Sbjct: 282 ILKNFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHN 341

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRVVSRLN+NPGFN TVLPPSAFYPVVWSRIR LFQ PKDA HLKW+ AKL+ I 
Sbjct: 342 GPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIRALFQSPKDAAHLKWVKAKLKHIE 401

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S+SLALHLWNSHSRKL+VEKGS+VD+I
Sbjct: 402 SHSLALHLWNSHSRKLQVEKGSVVDII 420

[Close]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL
Match: A0A5D3DSB7 (Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00610 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.4e-130
Identity = 225/265 (84.91%), Postives = 246/265 (92.83%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RELWAIQSIFK HE        NGNPCL+IVSNSLDSTKGKQ+L+P S+ GF ++AI
Sbjct: 152 FSDRELWAIQSIFKVHE--------NGNPCLIIVSNSLDSTKGKQVLSPFSEMGFSLLAI 211

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
           SP+FDSIF+NT+AE WFNQLRQGIIKPGEISLGQNLSNLLRL LLYKFGGIYID DVIIL
Sbjct: 212 SPDFDSIFKNTDAESWFNQLRQGIIKPGEISLGQNLSNLLRLTLLYKFGGIYIDADVIIL 271

Query: 137 KSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGP 196
           KSFTNLRNVIGAQT+DLKTGNWSRLNNAVMIFDKNHPLLLQFI+EFA+TFDGNKWGHNGP
Sbjct: 272 KSFTNLRNVIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGP 331

Query: 197 YLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRSN 256
           YLVSRV+SRLN+NPGFN T+LPPSAFYPVVW++I+TLFQGPKDAVHLKW+IAKLRQI+S 
Sbjct: 332 YLVSRVISRLNQNPGFNLTILPPSAFYPVVWNKIKTLFQGPKDAVHLKWVIAKLRQIQSK 391

Query: 257 SLALHLWNSHSRKLEVEKGSIVDVI 282
           SLALHLWNSHSRKLEVEKGSIVD+I
Sbjct: 392 SLALHLWNSHSRKLEVEKGSIVDII 408

[Close]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL
Match: A0A6J1KJE1 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111495773 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.4e-130
Identity = 229/267 (85.77%), Postives = 241/267 (90.26%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE WAIQSIFKAHE        NGNPCLVIVSNSLDSTKGKQIL+P S+KGFP+I
Sbjct: 175 NSFSDREFWAIQSIFKAHE--------NGNPCLVIVSNSLDSTKGKQILSPFSEKGFPLI 234

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
            ISP+FDSIFRNTEAEPWFNQLRQGIIKPGEISL QNLSNLLRLALLY FGGIY+D DVI
Sbjct: 235 VISPDFDSIFRNTEAEPWFNQLRQGIIKPGEISLAQNLSNLLRLALLYNFGGIYLDADVI 294

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEF +TFDGNKWGHN
Sbjct: 295 ILKNFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFTTTFDGNKWGHN 354

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRVVSRLN+NPGFN TVLPPSAFYPVVWSRIR LFQ PKDAVHLKW+ AKL+ I 
Sbjct: 355 GPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIRALFQSPKDAVHLKWVKAKLKHIE 414

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S SLALHLWNSHSRK +VEKGSIVD+I
Sbjct: 415 SQSLALHLWNSHSRKFQVEKGSIVDII 433

[Close]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL
Match: A0A1S3B637 (lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103486194 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.4e-130
Identity = 225/265 (84.91%), Postives = 246/265 (92.83%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RELWAIQSIFK HE        NGNPCL+IVSNSLDSTKGKQ+L+P S+ GF ++AI
Sbjct: 152 FSDRELWAIQSIFKVHE--------NGNPCLIIVSNSLDSTKGKQVLSPFSEMGFSLLAI 211

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
           SP+FDSIF+NT+AE WFNQLRQGIIKPGEISLGQNLSNLLRL LLYKFGGIYID DVIIL
Sbjct: 212 SPDFDSIFKNTDAESWFNQLRQGIIKPGEISLGQNLSNLLRLTLLYKFGGIYIDADVIIL 271

Query: 137 KSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGP 196
           KSFTNLRNVIGAQT+DLKTGNWSRLNNAVMIFDKNHPLLLQFI+EFA+TFDGNKWGHNGP
Sbjct: 272 KSFTNLRNVIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGP 331

Query: 197 YLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRSN 256
           YLVSRV+SRLN+NPGFN T+LPPSAFYPVVW++I+TLFQGPKDAVHLKW+IAKLRQI+S 
Sbjct: 332 YLVSRVISRLNQNPGFNLTILPPSAFYPVVWNKIKTLFQGPKDAVHLKWVIAKLRQIQSK 391

Query: 257 SLALHLWNSHSRKLEVEKGSIVDVI 282
           SLALHLWNSHSRKLEVEKGSIVD+I
Sbjct: 392 SLALHLWNSHSRKLEVEKGSIVDII 408

[Close]

BLAST of Lcy06g007200 vs. NCBI nr
Match: XP_022140162.1 (uncharacterized protein At4g19900, partial [Momordica charantia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 234/281 (83.27%), Postives = 257/281 (91.46%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RELWAIQS+FKAH        GNGN CLVIVSNSLDST+G+QIL+  S+ GF +IAI
Sbjct: 38  FSDRELWAIQSLFKAH--------GNGNSCLVIVSNSLDSTEGRQILSQFSENGFRVIAI 97

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
           SP+FDSIFR+TEAE WFNQLR+GIIKPGEI++GQNLSNLLRLALLYKFGGIYID DVIIL
Sbjct: 98  SPDFDSIFRDTEAEAWFNQLRRGIIKPGEIAIGQNLSNLLRLALLYKFGGIYIDADVIIL 157

Query: 137 KSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGP 196
           KSF NLRN IGAQT+DL+TGNWSRLNNAVMIFDKNHPLLL+FI+EFASTFDGN+WGHNGP
Sbjct: 158 KSFLNLRNAIGAQTVDLRTGNWSRLNNAVMIFDKNHPLLLEFIKEFASTFDGNRWGHNGP 217

Query: 197 YLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRSN 256
           YLVSRVVSRLN+NPGFNF+VLPPSAFYPVVWSR+R LFQGPKDAVH KW+IAKLRQIRS 
Sbjct: 218 YLVSRVVSRLNQNPGFNFSVLPPSAFYPVVWSRVRGLFQGPKDAVHSKWVIAKLRQIRSK 277

Query: 257 SLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCNYSASGLR 298
           SLALHLWNSHSRKLEVEKGSI+D+IRW+SCVFCN SAS LR
Sbjct: 278 SLALHLWNSHSRKLEVEKGSIIDLIRWNSCVFCNDSASRLR 310

[Close]

BLAST of Lcy06g007200 vs. NCBI nr
Match: KAG6584005.1 (hypothetical protein SDJN03_19937, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.4 bits (1230), Expect = 4.5e-131
Identity = 230/267 (86.14%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE WAIQSIFKAHE        NGNPCL+IVSNSLDSTKGKQIL+P S+KGFP+I
Sbjct: 175 NSFSDREFWAIQSIFKAHE--------NGNPCLIIVSNSLDSTKGKQILSPFSEKGFPLI 234

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           AISP+FDSIFRNTEAEPWFNQLRQGIIKPGEISL QNLSNLLRLALLYKFGGIY+D DVI
Sbjct: 235 AISPDFDSIFRNTEAEPWFNQLRQGIIKPGEISLAQNLSNLLRLALLYKFGGIYLDADVI 294

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNL+NVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFA+TFDGNKWGHN
Sbjct: 295 ILKNFTNLQNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHN 354

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRVVSRLN+NPGFN TVLPPSAFYPVVWSRIR LFQ PKDA HLKW+ AKL+ I 
Sbjct: 355 GPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIRALFQSPKDAAHLKWVKAKLKHIE 414

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S+SLALHLWNSHSRKL+VEKGSIVD+I
Sbjct: 415 SHSLALHLWNSHSRKLQVEKGSIVDII 433

[Close]

BLAST of Lcy06g007200 vs. NCBI nr
Match: XP_038905948.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Benincasa hispida])

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-130
Identity = 229/267 (85.77%), Postives = 247/267 (92.51%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RELWAIQS+FK HE        +GNPCLVIVSNSLDSTKGKQIL+P S+KGF + 
Sbjct: 167 NSFSDRELWAIQSVFKVHE--------HGNPCLVIVSNSLDSTKGKQILSPFSEKGFSLF 226

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           AISP+FDSIFRNTEAEPWFN LRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYID DVI
Sbjct: 227 AISPDFDSIFRNTEAEPWFNLLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDADVI 286

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNLRNVIGAQTI+LKTGNWSRLNNAVMIFDKNHPLLLQFIQEFA+TFDGNKWGHN
Sbjct: 287 ILKNFTNLRNVIGAQTINLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHN 346

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRV+SRLN+NPGFN TVLPPSAFYPVVW+RI++LFQGPKDAVHLKW+IAKL+ IR
Sbjct: 347 GPYLVSRVISRLNQNPGFNLTVLPPSAFYPVVWNRIKSLFQGPKDAVHLKWVIAKLKYIR 406

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S SLALHLWNSHSRKL+VEKGSIVD+I
Sbjct: 407 STSLALHLWNSHSRKLQVEKGSIVDII 425

[Close]

BLAST of Lcy06g007200 vs. NCBI nr
Match: XP_022927079.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata])

HSP 1 Score: 476.9 bits (1226), Expect = 1.3e-130
Identity = 229/267 (85.77%), Postives = 243/267 (91.01%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE WAIQSIFKAHE        NGNPCL IVSNSLDSTKGKQIL+P SKKGFP+I
Sbjct: 162 NSFSDREFWAIQSIFKAHE--------NGNPCLTIVSNSLDSTKGKQILSPFSKKGFPLI 221

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           AISP+FDSIFRNT+AEPWFNQLRQG IKPGEISL QNLSNLLRLALLYKFGGIY+D DVI
Sbjct: 222 AISPDFDSIFRNTQAEPWFNQLRQGTIKPGEISLAQNLSNLLRLALLYKFGGIYLDADVI 281

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFA+TFDGNKWGHN
Sbjct: 282 ILKNFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHN 341

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRVVSRLN+NPGFN TVLPPSAFYPVVWSRIR LFQ PKDA HLKW+ AKL+ I 
Sbjct: 342 GPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIRALFQSPKDAAHLKWVKAKLKHIE 401

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S+SLALHLWNSHSRKL+VEKGS+VD+I
Sbjct: 402 SHSLALHLWNSHSRKLQVEKGSVVDII 420

[Close]

BLAST of Lcy06g007200 vs. NCBI nr
Match: KAG7019628.1 (hypothetical protein SDJN02_18591, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 476.5 bits (1225), Expect = 1.7e-130
Identity = 229/267 (85.77%), Postives = 244/267 (91.39%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE WAIQSIFKAHE        NGNPCL+IVSNSLDS KGKQIL+P S+KGFP+I
Sbjct: 175 NSFSDREFWAIQSIFKAHE--------NGNPCLIIVSNSLDSAKGKQILSPFSEKGFPLI 234

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           AISP+FDSIFRNTEAEPWFNQLRQGIIKPGEISL QNLSNLLRLALLYKFGGIY+D DVI
Sbjct: 235 AISPDFDSIFRNTEAEPWFNQLRQGIIKPGEISLAQNLSNLLRLALLYKFGGIYLDADVI 294

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
           ILK+FTNL+NVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFA+TFDGNKWGHN
Sbjct: 295 ILKNFTNLQNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHN 354

Query: 195 GPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 254
           GPYLVSRVVSRLN+NPGFN TVLPPSAFYPVVWSRIR LFQ PKDA HLKW+ AKL+ I 
Sbjct: 355 GPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIRALFQSPKDAAHLKWVKAKLKHIE 414

Query: 255 SNSLALHLWNSHSRKLEVEKGSIVDVI 282
           S+SLALHLWNSHSRKL+VEKGSIVD+I
Sbjct: 415 SHSLALHLWNSHSRKLQVEKGSIVDII 433

[Close]

BLAST of Lcy06g007200 vs. TAIR 10
Match: AT1G61050.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 317.8 bits (813), Expect = 9.4e-87
Identity = 152/279 (54.48%), Postives = 198/279 (70.97%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RE + I+S+FK H           N CL++VSNS D  +G  IL P + KG  ++ I
Sbjct: 164 FGDRERFTIESLFKFHP----------NGCLILVSNSFDCDRGTLILKPFTDKGLKVLPI 223

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
            P+F  IF++T AE WF +L++G + PG I L QNLSNLLRL LLYK+GGIY+DTDVIIL
Sbjct: 224 KPDFAYIFKDTSAEKWFERLKKGTLSPGVIPLEQNLSNLLRLVLLYKYGGIYLDTDVIIL 283

Query: 137 KSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNGP 196
           KS +NL NVIGAQT+D  T  WSRLNNAV+IFDKNHPLL +FI EF+ TF+GNKWGHNGP
Sbjct: 284 KSLSNLHNVIGAQTVDPVTKKWSRLNNAVLIFDKNHPLLKRFIDEFSRTFNGNKWGHNGP 343

Query: 197 YLVSRVVSR--LNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIR 256
           YLVSRV++R  ++ +    F+VLPPSAFYPV W+RI+  ++ P +     WL  +L  +R
Sbjct: 344 YLVSRVITRIKISSSSDLGFSVLPPSAFYPVDWTRIKGFYRAPTNESD-AWLRKRLTHLR 403

Query: 257 SNSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCNYSA 294
            N+ A+HLWN  S+KL +E+GSI+  +   SC+FCN S+
Sbjct: 404 KNTFAVHLWNRESKKLRIEEGSIIHQLMSHSCIFCNSSS 431

[Close]

BLAST of Lcy06g007200 vs. TAIR 10
Match: AT5G01250.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.2e-79
Identity = 139/288 (48.26%), Postives = 194/288 (67.36%), Query Frame = 0


Query: 6   TPIDFHYDYNLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTP 65
           +P DF      F +RE+ AI+S+FK+H             CL+I+S ++DS +G   L P
Sbjct: 133 SPADF------FGNREVLAIESVFKSHP----------YGCLMILSATMDSPQGYATLKP 192

Query: 66  ISKKGFPIIAISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFG 125
              +G+ ++A++P+   + + T  E W ++++ G   PG+ISL QNLSNL+RLA LYK+G
Sbjct: 193 FIDRGYKVLAVTPDLPFLLKGTAGELWLDEIKSGKRDPGKISLAQNLSNLMRLAYLYKYG 252

Query: 126 GIYIDTDVIILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFAST 185
           G+Y+DTD+I+LKSF  LRNVIGAQT+D  + NW+RLNNAV+IFDKNHPLLL+F++EFA T
Sbjct: 253 GVYLDTDMIVLKSFKGLRNVIGAQTLDPSSTNWTRLNNAVLIFDKNHPLLLKFMEEFAKT 312

Query: 186 FDGNKWGHNGPYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKW 245
           F+GN WG+NGPYLVSRV   +  + G+NFTV+ PS FY V W  I+ LF+ PK     KW
Sbjct: 313 FNGNIWGYNGPYLVSRVARAVEGSSGYNFTVMRPSVFYSVNWLEIKKLFKVPKTEKDSKW 372

Query: 246 LIAKLRQIRSNSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCNYSA 294
           +  KL  ++ N   LHLWN  SRK E+E+GS +  +    C+ C  S+
Sbjct: 373 VKTKLLHMQRNGYGLHLWNKFSRKYEIEQGSAMWKLVSEHCIICEISS 404

[Close]

BLAST of Lcy06g007200 vs. TAIR 10
Match: AT3G09020.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.1e-78
Identity = 133/275 (48.36%), Postives = 191/275 (69.45%), Query Frame = 0


Query: 16  LFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIA 75
           LF  RE+ +++S+FK+H             CL+I+S+++DS +G +IL P   +G+ ++A
Sbjct: 141 LFGKREILSVESVFKSH----------ARGCLMILSSTMDSLQGFRILKPFLDRGYRVMA 200

Query: 76  ISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVII 135
           ++P+   + ++T  E W  +++ G   PG+ISL QNLSNL+RLA L+KFGG+Y+DTD+I+
Sbjct: 201 VTPDLPFLLKDTAGESWLEEIQTGKRDPGKISLAQNLSNLMRLAYLFKFGGVYLDTDMIV 260

Query: 136 LKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNG 195
           LKSF  LRNVIGAQT++  + NW+RLNNAV+IFDKNHP LL+ I+EFA TF+GN WGHNG
Sbjct: 261 LKSFKTLRNVIGAQTLEPVSRNWTRLNNAVLIFDKNHPFLLKSIEEFALTFNGNVWGHNG 320

Query: 196 PYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRS 255
           PYLVSRV   +    G+NFT+L P AFYPV W  I  LF+ P+     K +  K+ +++ 
Sbjct: 321 PYLVSRVARAVEGTDGYNFTILTPPAFYPVNWVEIEKLFKVPRTEKDSKRVQVKVLEMQK 380

Query: 256 NSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFCN 291
            S  LHLWN  SRK E+E+GS +D +  + C+ C+
Sbjct: 381 RSYGLHLWNKFSRKFEIEQGSAMDKLVSNQCIICD 405

[Close]

BLAST of Lcy06g007200 vs. TAIR 10
Match: AT2G38152.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.8e-77
Identity = 138/274 (50.36%), Postives = 182/274 (66.42%), Query Frame = 0


Query: 17  FPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPIIAI 76
           F  RE+ A++S+FKAH             CL+IVS SLDS +G  IL P++ +G+ + A 
Sbjct: 112 FGKREMLAVESVFKAHP----------QGCLMIVSGSLDSLQGDSILKPLNDRGYKVFAA 171

Query: 77  SPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVIIL 136
           +P+   +  NT A+ WF +++     PG I L QNLSNL RLA LYK+GG+Y+DTD I+ 
Sbjct: 172 TPDMSLLLENTPAKSWFQEMKSCKRDPGRIPLHQNLSNLARLAFLYKYGGVYLDTDFIVT 231

Query: 137 KSFTNLRNVIGAQT-IDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHNG 196
           +SF  L+N IGAQT ++  + NW+RLNNAV+IF+K+HPL+  FI+EFASTFDGNKWGHNG
Sbjct: 232 RSFKGLKNSIGAQTVVEGDSKNWTRLNNAVLIFEKDHPLVYSFIEEFASTFDGNKWGHNG 291

Query: 197 PYLVSRVVSRLNENPGFNFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQIRS 256
           PYLV+RV  R  E  G NFTVLPP AFYP  W  I  LFQ P+ +     L   L ++  
Sbjct: 292 PYLVTRVAQRARETIGDNFTVLPPVAFYPFNWLDIPRLFQTPRGSNDSTLLKTDLVKLNR 351

Query: 257 NSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFC 290
            S  LHLWN  +RKL++ KGS++D+I    CV C
Sbjct: 352 ESYGLHLWNKITRKLKIGKGSVIDIIISDHCVVC 375

[Close]

BLAST of Lcy06g007200 vs. TAIR 10
Match: AT2G38150.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 272.3 bits (695), Expect = 4.5e-73
Identity = 128/276 (46.38%), Postives = 178/276 (64.49%), Query Frame = 0


Query: 15  NLFPSRELWAIQSIFKAHENETPNENGNGNPCLVIVSNSLDSTKGKQILTPISKKGFPII 74
           N F  RE+ AI ++F            N   CL I+SNSLDS  G  IL P+  +GF +I
Sbjct: 132 NSFGPREMLAIDTLFTT----------NPGACLAILSNSLDSPNGYTILKPLFDQGFNLI 191

Query: 75  AISPNFDSIFRNTEAEPWFNQLRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDTDVI 134
           A++ +   + +NT AE W  +L+ G + PG I L  NLS+L RLA+LYK+GG+Y+DTD+I
Sbjct: 192 AVTIDIPFLVKNTPAEAWLKRLKSGNMDPGSIPLFMNLSDLTRLAVLYKYGGVYLDTDII 251

Query: 135 ILKSFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKNHPLLLQFIQEFASTFDGNKWGHN 194
            L   T LRN IGAQ+ D  T  W+RLNNAVM+FD  HPL+ +F+QE+A+TFDGNKWG+N
Sbjct: 252 FLNDMTGLRNAIGAQSSDPATKRWTRLNNAVMVFDIYHPLMREFLQEYATTFDGNKWGYN 311

Query: 195 GPYLVSRVVSRLNENPGF-NFTVLPPSAFYPVVWSRIRTLFQGPKDAVHLKWLIAKLRQI 254
            PYLVSRV+ RL   PG+ N T+  P AFYPV W +I+ LF+ P      KW+   ++ +
Sbjct: 312 SPYLVSRVIKRLGNKPGYNNLTIFSPDAFYPVNWIKIQKLFKKPATTREAKWVEKTVQDM 371

Query: 255 RSNSLALHLWNSHSRKLEVEKGSIVDVIRWSSCVFC 290
              S  +HLWN  +RK+++E+GS++  +  + C  C
Sbjct: 372 NKGSYMIHLWNKVTRKIKIEEGSVMHTLVSTHCTVC 397

The following BLAST results are available for this feature:

BLAST of Lcy06g007200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67BJ4	2.2e-24	31.31	Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4gal...	[more]
Q9JI93	2.9e-24	32.71	Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=...	[more]
P0C8Q4	5.0e-24	29.23	Uncharacterized protein At4g19900 OS=Arabidopsis thaliana OX=3702 GN=At4g19900 P...	[more]
Q9NPC4	3.2e-23	32.24	Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT...	[more]
Q9N289	7.2e-23	31.78	Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Pongo pygmaeus OX=9...	[more]

BLAST of Lcy06g007200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CG17	7.2e-135	83.27	uncharacterized protein At4g19900 OS=Momordica charantia OX=3673 GN=LOC111010894...	[more]
A0A6J1EH01	6.3e-131	85.77	lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=366...	[more]
A0A5D3DSB7	2.4e-130	84.91	Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=11...	[more]
A0A6J1KJE1	2.4e-130	85.77	lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A1S3B637	2.4e-130	84.91	lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Lcy06g007200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140162.1	1.5e-134	83.27	uncharacterized protein At4g19900, partial [Momordica charantia]	[more]
KAG6584005.1	4.5e-131	86.14	hypothetical protein SDJN03_19937, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038905948.1	1.0e-130	85.77	lactosylceramide 4-alpha-galactosyltransferase-like [Benincasa hispida]	[more]
XP_022927079.1	1.3e-130	85.77	lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata]	[more]
KAG7019628.1	1.7e-130	85.77	hypothetical protein SDJN02_18591, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lcy06g007200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61050.1	9.4e-87	54.48	alpha 1,4-glycosyltransferase family protein	[more]
AT5G01250.1	3.2e-79	48.26	alpha 1,4-glycosyltransferase family protein	[more]
AT3G09020.1	2.1e-78	48.36	alpha 1,4-glycosyltransferase family protein	[more]
AT2G38152.1	8.8e-77	50.36	alpha 1,4-glycosyltransferase family protein	[more]
AT2G38150.1	4.5e-73	46.38	alpha 1,4-glycosyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.550.20		coord: 29..216 e-value: 1.8E-24 score: 88.7
None	No IPR available	PANTHER	PTHR46781:SF5	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 16..294
IPR007577	Glycosyltransferase, DXD sugar-binding motif	PFAM	PF04488	Gly_transf_sug	coord: 19..150 e-value: 5.7E-17 score: 62.2
IPR007652	Alpha 1,4-glycosyltransferase domain	PFAM	PF04572	Gb3_synth	coord: 168..290 e-value: 1.5E-27 score: 96.4
IPR044789	Putative alpha 1,4-glycosyltransferase, plant	PANTHER	PTHR46781	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 16..294
IPR029044	Nucleotide-diphospho-sugar transferases	SUPERFAMILY	53448	Nucleotide-diphospho-sugar transferases	coord: 54..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy06g007200.1	Lcy06g007200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016757	glycosyltransferase activity