Lcy06g006970 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g006970
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr06: 6723717 .. 6724137 (-)
RNA-Seq ExpressionLcy06g006970
SyntenyLcy06g006970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGATTTGGCGCTTGTAGCGGTGACGTGCTGGGCCATTTGGATGGACAGAATCAAGAAAGCTCATGGAGAAGAAATACCTCCAGTTAGTATTCGAAGCAATTCGATCGCAAATTATTTAGAAGATTATCAAAAGGCTAACCTGATTCCATCAACTTCATTCTGCGGTGACCTCTGCATGCTGGATTCCCCCTAAAGATGGAGTCTTAAAAATTAATGTCGATGCGGCTTGGTCTGCAAACCCCCAAAGAATTGGTCATGGAGCTCTCTTTCGAGATTCAAGAGGTTTTCTGAAGGGAGCCTTGGTGTCGTCGATTGACCTAGATTTTTTACCCCCTTTAGCAGAGTTGAGGGCGATTTTGGATGGTTTGAAGCTGGCTAAACATCTTCTTTGCTCTAAAATCATTTGTGGAATCTGA

mRNA sequence

ATGGAGGATTTGGCGCTTGTAGCGGTGACGTGCTGGGCCATTTGGATGGACAGAATCAAGAAAGCTCATGGAGAAGAAATACCTCCAGTTAGTATTCGAAGCAATTCGATCGCAAATTATTTAGAAGATTATCAAAAGGCTAACCTGATTCCATCAACTTCATTCTGCGATGGAGTCTTAAAAATTAATGTCGATGCGGCTTGGTCTGCAAACCCCCAAAGAATTGGTCATGGAGCTCTCTTTCGAGATTCAAGAGGTTTTCTGAAGGGAGCCTTGGTGTCGTCGATTGACCTAGATTTTTTACCCCCTTTAGCAGAGTTGAGGGCGATTTTGGATGGTTTGAAGCTGGCTAAACATCTTCTTTGCTCTAAAATCATTTGTGGAATCTGA

Coding sequence (CDS)

ATGGAGGATTTGGCGCTTGTAGCGGTGACGTGCTGGGCCATTTGGATGGACAGAATCAAGAAAGCTCATGGAGAAGAAATACCTCCAGTTAGTATTCGAAGCAATTCGATCGCAAATTATTTAGAAGATTATCAAAAGGCTAACCTGATTCCATCAACTTCATTCTGCGATGGAGTCTTAAAAATTAATGTCGATGCGGCTTGGTCTGCAAACCCCCAAAGAATTGGTCATGGAGCTCTCTTTCGAGATTCAAGAGGTTTTCTGAAGGGAGCCTTGGTGTCGTCGATTGACCTAGATTTTTTACCCCCTTTAGCAGAGTTGAGGGCGATTTTGGATGGTTTGAAGCTGGCTAAACATCTTCTTTGCTCTAAAATCATTTGTGGAATCTGA

Protein sequence

MEDLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQKANLIPSTSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELRAILDGLKLAKHLLCSKIICGI
Homology
BLAST of Lcy06g006970 vs. ExPASy TrEMBL
Match: A0A0Q3GGJ7 (RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_2g53033v3 PE=4 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.4e-10
Identity = 44/128 (34.38%), Postives = 68/128 (53.12%), Query Frame = 0

Query: 6   LVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSI----ANYLEDYQKANLIPSTSF---CDG 65
           L AV CW IW +R +  HGE IPP    + SI     NY+  Y K + I    +    +G
Sbjct: 91  LCAVACWYIWWERRQLMHGEPIPPPDTTAFSIQALVMNYVRSYSKQSGIQRLGWQKPPEG 150

Query: 66  VLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELRAILDGLKLAK 125
           + K+NVDA++  + +    GA+ RD+ G    A  S I + +   +AE  A+ +G++ AK
Sbjct: 151 MQKLNVDASFIEDKEEGATGAVIRDASGLFVRASNSCIPIVYDATMAEAMALWNGIQFAK 210

Query: 126 HLLCSKII 127
            L CS ++
Sbjct: 211 SLGCSNLL 218

BLAST of Lcy06g006970 vs. ExPASy TrEMBL
Match: A0A6J1CK80 (uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012047 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.0e-10
Identity = 46/133 (34.59%), Postives = 70/133 (52.63%), Query Frame = 0

Query: 1   MEDLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANY----LEDYQKANLIPSTSFC 60
           +E+L L  + CWA W DR ++ +G ++  V   S+ I NY    L   +K N       C
Sbjct: 25  LEELLLAGIICWANWNDRNQRNNGIDVADVHTESDWITNYAMELLTRRRKTNEAQKNLPC 84

Query: 61  --------DGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELR 120
                    G +K+NVDAA+    QR G G L RD R  +  AL+SS +++  P LAE+ 
Sbjct: 85  LAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDRNSILAALISSHNVN-SPLLAEIC 144

Query: 121 AILDGLKLAKHLL 122
           AI + ++LA+  +
Sbjct: 145 AIREAVRLAERCI 156

BLAST of Lcy06g006970 vs. ExPASy TrEMBL
Match: A0A803NM27 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.5e-08
Identity = 44/134 (32.84%), Postives = 64/134 (47.76%), Query Frame = 0

Query: 3    DLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQK-------ANLIPS--- 62
            +L ++  T W+IW DR    HG+     S+ S   A++L  +Q        A L P+   
Sbjct: 1556 ELEMIFCTMWSIWSDRNNVLHGKIPQQPSVISAKAASFLSSFQSAQQLSLHAGLAPADTP 1615

Query: 63   ------TSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAE 121
                  T     +LK+NVDAA+    +RIG GA+ RDS G +K A+   ID    P   E
Sbjct: 1616 TAHRAWTPPPPNLLKLNVDAAFDDTRKRIGFGAIIRDSTGNVKAAMSHPIDGCCRPQDME 1675

BLAST of Lcy06g006970 vs. ExPASy TrEMBL
Match: A0A803QAH8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.5e-08
Identity = 44/134 (32.84%), Postives = 64/134 (47.76%), Query Frame = 0

Query: 3    DLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQKAN-------LIPS--- 62
            +L ++  T W+IW DR    HG+     S+ S   A++L  +Q A+       L P+   
Sbjct: 1543 ELEMIFCTMWSIWSDRNNVIHGKLAQQPSVISAKAASFLSSFQSAHQLSLHAGLAPADTP 1602

Query: 63   ------TSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAE 121
                  T      LK+NVDAA+    +RIG GA+ RDS G +K A+   ID    P   E
Sbjct: 1603 TAHKTWTPPPPTFLKLNVDAAFDDTRKRIGFGAIIRDSTGKVKAAMSHPIDGCCRPQDME 1662

BLAST of Lcy06g006970 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.2e-07
Identity = 42/134 (31.34%), Postives = 60/134 (44.78%), Query Frame = 0

Query: 2    EDLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQKANLI----------- 61
            +DL L A+T W IW DR    HG+++ PV  +   +  +L+ + +A +            
Sbjct: 1229 KDLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHR 1288

Query: 62   -------PSTSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPP 118
                   PS+S     LK+N DAA          G + RDS   L  A    +     P 
Sbjct: 1289 PVVQYWRPSSSVS---LKLNTDAA--CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPL 1348

BLAST of Lcy06g006970 vs. NCBI nr
Match: XP_024315667.1 (uncharacterized protein LOC112271019 [Brachypodium distachyon] >KQK10208.2 hypothetical protein BRADI_2g53033v3 [Brachypodium distachyon])

HSP 1 Score: 75.1 bits (183), Expect = 5.0e-10
Identity = 44/128 (34.38%), Postives = 68/128 (53.12%), Query Frame = 0

Query: 6   LVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSI----ANYLEDYQKANLIPSTSF---CDG 65
           L AV CW IW +R +  HGE IPP    + SI     NY+  Y K + I    +    +G
Sbjct: 91  LCAVACWYIWWERRQLMHGEPIPPPDTTAFSIQALVMNYVRSYSKQSGIQRLGWQKPPEG 150

Query: 66  VLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELRAILDGLKLAK 125
           + K+NVDA++  + +    GA+ RD+ G    A  S I + +   +AE  A+ +G++ AK
Sbjct: 151 MQKLNVDASFIEDKEEGATGAVIRDASGLFVRASNSCIPIVYDATMAEAMALWNGIQFAK 210

Query: 126 HLLCSKII 127
            L CS ++
Sbjct: 211 SLGCSNLL 218

BLAST of Lcy06g006970 vs. NCBI nr
Match: XP_022141766.1 (uncharacterized protein LOC111012047 [Momordica charantia])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 46/133 (34.59%), Postives = 70/133 (52.63%), Query Frame = 0

Query: 1   MEDLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANY----LEDYQKANLIPSTSFC 60
           +E+L L  + CWA W DR ++ +G ++  V   S+ I NY    L   +K N       C
Sbjct: 25  LEELLLAGIICWANWNDRNQRNNGIDVADVHTESDWITNYAMELLTRRRKTNEAQKNLPC 84

Query: 61  --------DGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELR 120
                    G +K+NVDAA+    QR G G L RD R  +  AL+SS +++  P LAE+ 
Sbjct: 85  LAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDRNSILAALISSHNVN-SPLLAEIC 144

Query: 121 AILDGLKLAKHLL 122
           AI + ++LA+  +
Sbjct: 145 AIREAVRLAERCI 156

BLAST of Lcy06g006970 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 66.6 bits (161), Expect = 1.8e-07
Identity = 44/128 (34.38%), Postives = 61/128 (47.66%), Query Frame = 0

Query: 6   LVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQKA----------NLIPSTSF 65
           L A T WAIW DR   AHG  +   ++R N I++Y ++Y +A          + +P  S 
Sbjct: 54  LAAFTXWAIWCDRNSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPS- 113

Query: 66  C------DGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELRA 118
           C      D  +K+N DAA        G G + RD  G L  A    + +   P  AE+R 
Sbjct: 114 CRWLPPRDSAMKVNSDAA--CRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRG 173

BLAST of Lcy06g006970 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 65.5 bits (158), Expect = 3.9e-07
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 51  PSTSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPPLAELRAI 110
           P TSF    +K+NVDAAW  +P   G  A+ RD++G LK   +SSID  + PPLAE   +
Sbjct: 59  PPTSF----IKLNVDAAWKFSPYSSGFSAIIRDNQGSLKVVQISSIDAVYPPPLAEAFVV 118

Query: 111 LDGLKLAKHLLCSKII 127
           L GL+L   +   KII
Sbjct: 119 LQGLRLTSKMNFKKII 130

BLAST of Lcy06g006970 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 64.7 bits (156), Expect = 6.7e-07
Identity = 42/134 (31.34%), Postives = 60/134 (44.78%), Query Frame = 0

Query: 2    EDLALVAVTCWAIWMDRIKKAHGEEIPPVSIRSNSIANYLEDYQKANLI----------- 61
            +DL L A+T W IW DR    HG+++ PV  +   +  +L+ + +A +            
Sbjct: 1229 KDLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHR 1288

Query: 62   -------PSTSFCDGVLKINVDAAWSANPQRIGHGALFRDSRGFLKGALVSSIDLDFLPP 118
                   PS+S     LK+N DAA          G + RDS   L  A    +     P 
Sbjct: 1289 PVVQYWRPSSSVS---LKLNTDAA--CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPL 1348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0Q3GGJ72.4e-1034.38RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_2... [more]
A0A6J1CK807.0e-1034.59uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A803NM276.5e-0832.84Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QAH88.5e-0832.84Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DX303.2e-0731.34uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
Match NameE-valueIdentityDescription
XP_024315667.15.0e-1034.38uncharacterized protein LOC112271019 [Brachypodium distachyon] >KQK10208.2 hypot... [more]
XP_022141766.11.4e-0934.59uncharacterized protein LOC111012047 [Momordica charantia][more]
XP_022131661.11.8e-0734.38uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_038902513.13.9e-0746.05uncharacterized protein LOC120089172 [Benincasa hispida][more]
XP_022158377.16.7e-0731.34uncharacterized protein LOC111024874 [Momordica charantia][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 63..126
e-value: 1.1E-6
score: 28.5
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 62..127
e-value: 1.06376E-11
score: 55.7832
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 59..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g006970.1Lcy06g006970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity