Lcy06g005860 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g005860
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description5'-adenylylsulfate reductase 1
LocationChr06: 5556073 .. 5559000 (-)
RNA-Seq ExpressionLcy06g005860
SyntenyLcy06g005860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAATTACGATTAACCGAAAGAGAGAGCAAGATTCTTTTGTCCTTCATGGCGCTTCCTGCTGCTTCTTCTTCTTCTTCTTCAATTTCCGCTTCCAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGGTATTGTTCAGAAATATGGTCTCCTGTTTACATTGTGAAGCGATGTCGAAGGTTTTTGTCGTGTTTGAATGGGTTTTTTTTTTTTGAAGAATTGCTGTTGAATACAATGTTGTTTCTTAATTGTGGATATCGTTTCGATTTGCAGCTCCTCGAATTGGTTCTTTTCAGCTGTTGAATCGGCCGCACTTTGTATCTGCTGGTGTCAATTATTCATTAAGAGGATGCTCGGTGAAGCCTATTAATGCGCAATCCAAACGGAATGATTCGCTTGTTCCTCTGGCAGCGAATACCCTGAATGCTGGTAATTCTGATTTCTTAGTAGCCCTATCTCATGTTCTTTTATATTTTCAAGAATCCGAGTTCAGTTCTGGGAATTTGTGTAAAAGAATGTTTCTGGTGTGGATAATGATGATGTTTTGGGGTTGGATCTGCAGAGGTGGCAGATAAAGCAGAGACGGTAGACTATGATGGATTAGCCAAAGACCTTGAAAATGCTTCTCCTCTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGGTTTGTTGTTATTGGACCATTTGATTAAATTGCGTCTTTAGCTTTTATTATCCAACATCCTGTCTCAAATGTCTAAAGTTCAAATTGCAATGCTGTTGAATCTATGATCTGTAAGGTGTCTCTTTAATCATATATATCATTGTCGTTCGAGGATAAGACCATTTTCAAGCATCTCCTTACCATAGAGATTCGTATTACCAGAGTTTATTTATTCTCTCTGCCGCCACCTGTTCCCATTTCTTTAATTTTCCACAAGCTTTTAGCTACGATCAAATGTAGATGGTAGAGCATAATGAAACACAATGGAAATGTTGAAGAGAAGTGTCAACCGAGTAAAGCTTGGATCAATTTTGTTGAAATTCATAATTAACACACATAATAAACTAATAATATTAGATTTCGGGTTTTTATTTTCCTTTTATTATCAGCAAAGTATATTTCCGACCTTCTTTCTGAACACTGAATTATAAATTCAAGGGTATTAATTTCATTCTTCGCCAATAATTTACTAGTAAAGCATTTGGTTTCTGATTCTTGGTCAGTTAAGTTGTAAAGCATCTCCTTGTGCTCTTCTTACCACAAAACATCTAACAAAAGTTATCCAATGCAGTGGCGCCGAAGATGTCGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCATCAGGAGTGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGACTCCGAGCATGGATCACTGGACAAAGAAAAGACCAATCTCCTGGAACAAGGTCTGAGATTCCTGTTGTCCAAGTTGATCCTGTTTTTGAGGGGTTGGACGGGGGCGTTGGGAGCTTGATCAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTTCCTGTGAACTCTTTGCATTCTCAAGGATACGTCTCGATTGGCTGTGAGCCGTGCACGAGGCCTGTCCTCCCAGGTCAGCACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCTAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAGGAAGATGTTGCCCAGCTCAATGGCAATGGACACGTAAATGGATCTCCCCCAGTTAATGACATCTTCATCTCCCCAAATTTGGTGAACCTGAACAGAACTGGAATAGAAAACTTGGCCAGGCTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGTAAAAATTCATTTTGCATGATAACGTTTCAGTTGATAATCGTAATGGTAACAACAAAGTTTTAGTTTGGAATATCATCACTAAATGACATTTTTGGTCGAATTTCTACAGGCGATGGAAGGGTCATATGTTGAATTGGCTGAGAAGTTGGCTGGGAGTGGAGTGAAAGTTGGAAAGTTCAGATCCGACGGCGAGCAGAAGGAGTTTGCCAAACGAGAGCTGCAGCTAGGATCCTTCCCCACCATTCTCTTTTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCTGAGAAGAGGGACGTTGATTCGTTGATGGCTTTTGTGAATGCCCTACGATGATATATACAAAAATATATGATATAGCAAAAGTCAAGTCCTAAATTCCTAAAATACGGTCTGATCGCTATTAACGAAGGAAAAATTCACACAGGCTCCAATCGTTTGTTGGATACTGTATACATCTGAGGAAGGATAACAAACCACTACAGTGGAAGATGAGATTAAAATCAAAGCTTGGGCATCTTTTTGAAGCTCAGAGCATTCGACCCTGATATCTTCCGATTATGTTTTTGTTATTCGTGGCATGTACATGTATATATCAGTTTTTCCGTAGGCTCCCACAACAACGATGTAGTAATGATCCAACGGATGTAGGTTCTAGATTGTTTTATCAGAGGATTTGTTTGATCTTGTGTCTCTTTTGGTTGTACTAATTTACGTTTGTGCTATTCGAGGAAGCCATCACAGTGTGACAGTCAGTTTTTCAGTTTTTTCCTCATAAATTTGAATCAATTAAGGGAAAATCATACAGATTTTCGA

mRNA sequence

GAAGAATTACGATTAACCGAAAGAGAGAGCAAGATTCTTTTGTCCTTCATGGCGCTTCCTGCTGCTTCTTCTTCTTCTTCTTCAATTTCCGCTTCCAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGCTCCTCGAATTGGTTCTTTTCAGCTGTTGAATCGGCCGCACTTTGTATCTGCTGGTGTCAATTATTCATTAAGAGGATGCTCGGTGAAGCCTATTAATGCGCAATCCAAACGGAATGATTCGCTTGTTCCTCTGGCAGCGAATACCCTGAATGCTGAGGTGGCAGATAAAGCAGAGACGGTAGACTATGATGGATTAGCCAAAGACCTTGAAAATGCTTCTCCTCTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGTGGCGCCGAAGATGTCGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCATCAGGAGTGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGACTCCGAGCATGGATCACTGGACAAAGAAAAGACCAATCTCCTGGAACAAGGTCTGAGATTCCTGTTGTCCAAGTTGATCCTGTTTTTGAGGGGTTGGACGGGGGCGTTGGGAGCTTGATCAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTTCCTGTGAACTCTTTGCATTCTCAAGGATACGTCTCGATTGGCTGTGAGCCGTGCACGAGGCCTGTCCTCCCAGGTCAGCACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCTAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAGGAAGATGTTGCCCAGCTCAATGGCAATGGACACGTAAATGGATCTCCCCCAGTTAATGACATCTTCATCTCCCCAAATTTGGTGAACCTGAACAGAACTGGAATAGAAAACTTGGCCAGGCTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGCGATGGAAGGGTCATATGTTGAATTGGCTGAGAAGTTGGCTGGGAGTGGAGTGAAAGTTGGAAAGTTCAGATCCGACGGCGAGCAGAAGGAGTTTGCCAAACGAGAGCTGCAGCTAGGATCCTTCCCCACCATTCTCTTTTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCTGAGAAGAGGGACGTTGATTCGTTGATGGCTTTTGTGAATGCCCTACGATGATATATACAAAAATATATGATATAGCAAAAGTCAAGTCCTAAATTCCTAAAATACGGTCTGATCGCTATTAACGAAGGAAAAATTCACACAGGCTCCAATCGTTTGTTGGATACTGTATACATCTGAGGAAGGATAACAAACCACTACAGTGGAAGATGAGATTAAAATCAAAGCTTGGGCATCTTTTTGAAGCTCAGAGCATTCGACCCTGATATCTTCCGATTATGTTTTTGTTATTCGTGGCATGTACATGTATATATCAGTTTTTCCGTAGGCTCCCACAACAACGATGTAGTAATGATCCAACGGATGTAGGTTCTAGATTGTTTTATCAGAGGATTTGTTTGATCTTGTGTCTCTTTTGGTTGTACTAATTTACGTTTGTGCTATTCGAGGAAGCCATCACAGTGTGACAGTCAGTTTTTCAGTTTTTTCCTCATAAATTTGAATCAATTAAGGGAAAATCATACAGATTTTCGA

Coding sequence (CDS)

ATGGCGCTTCCTGCTGCTTCTTCTTCTTCTTCTTCAATTTCCGCTTCCAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGCTCCTCGAATTGGTTCTTTTCAGCTGTTGAATCGGCCGCACTTTGTATCTGCTGGTGTCAATTATTCATTAAGAGGATGCTCGGTGAAGCCTATTAATGCGCAATCCAAACGGAATGATTCGCTTGTTCCTCTGGCAGCGAATACCCTGAATGCTGAGGTGGCAGATAAAGCAGAGACGGTAGACTATGATGGATTAGCCAAAGACCTTGAAAATGCTTCTCCTCTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGTGGCGCCGAAGATGTCGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCATCAGGAGTGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGACTCCGAGCATGGATCACTGGACAAAGAAAAGACCAATCTCCTGGAACAAGGTCTGAGATTCCTGTTGTCCAAGTTGATCCTGTTTTTGAGGGGTTGGACGGGGGCGTTGGGAGCTTGATCAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTTCCTGTGAACTCTTTGCATTCTCAAGGATACGTCTCGATTGGCTGTGAGCCGTGCACGAGGCCTGTCCTCCCAGGTCAGCACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCTAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAGGAAGATGTTGCCCAGCTCAATGGCAATGGACACGTAAATGGATCTCCCCCAGTTAATGACATCTTCATCTCCCCAAATTTGGTGAACCTGAACAGAACTGGAATAGAAAACTTGGCCAGGCTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGCGATGGAAGGGTCATATGTTGAATTGGCTGAGAAGTTGGCTGGGAGTGGAGTGAAAGTTGGAAAGTTCAGATCCGACGGCGAGCAGAAGGAGTTTGCCAAACGAGAGCTGCAGCTAGGATCCTTCCCCACCATTCTCTTTTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCTGAGAAGAGGGACGTTGATTCGTTGATGGCTTTTGTGAATGCCCTACGATGA

Protein sequence

MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Homology
BLAST of Lcy06g005860 vs. ExPASy Swiss-Prot
Match: P92979 (5'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR1 PE=1 SV=2)

HSP 1 Score: 702.2 bits (1811), Expect = 3.9e-201
Identity = 351/471 (74.52%), Postives = 400/471 (84.93%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYS-LRGCSVKP 60
           M++  +SSSSS I  S    F  S E K  +IGS +LL+R H     +N S  R  SVKP
Sbjct: 3   MSVNVSSSSSSGIINS---RFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKP 62

Query: 61  INAQSKRNDSLVPLAANTLNAEVADKAETV---DYDGLAKDLENASPLEIMDKALEKFGN 120
           +NA+ K  DS++PLAA T+ AE+A++ E V   D++ LAK LENASPLEIMDKALEK+GN
Sbjct: 63  LNAEPKTKDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGN 122

Query: 121 DIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPD 180
           DIAIAFSGAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD
Sbjct: 123 DIAIAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPD 182

Query: 181 AVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIP 240
           +VEVQGLVR+KGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP
Sbjct: 183 SVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIP 242

Query: 241 VVQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCT 300
           VVQVDPVFEGLDGGVGSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT
Sbjct: 243 VVQVDPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCT 302

Query: 301 RPVLPGQHEREGRWWWEDSKAKECGLHKGNLKQ-EDVAQLNGNGHVNGSPPVNDIFISPN 360
           + VLPGQHEREGRWWWED+KAKECGLHKGN+K+  D A++NG         V DIF S N
Sbjct: 303 KAVLPGQHEREGRWWWEDAKAKECGLHKGNVKENSDDAKVNG----ESKSAVADIFKSEN 362

Query: 361 LVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRS 420
           LV L+R GIENL +LENRKEPW+VVLYAPWC FCQAME SY ELA+KLAGSG+KV KFR+
Sbjct: 363 LVTLSRQGIENLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRA 422

Query: 421 DGEQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           DG+QKEFAK+ELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL +F+N +R
Sbjct: 423 DGDQKEFAKQELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Lcy06g005860 vs. ExPASy Swiss-Prot
Match: P92980 (5'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR3 PE=2 SV=2)

HSP 1 Score: 687.6 bits (1773), Expect = 1.0e-196
Identity = 341/466 (73.18%), Postives = 389/466 (83.48%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           +A+  +SSSSS+IS+S+      SS+ K  +IGS +LLNR +  +A ++ S +  SVK +
Sbjct: 3   LAINVSSSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKAL 62

Query: 61  NAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAI 120
           N QS   +S+V         E  D  E  D++ LAK LENASPLEIMDKALEKFGNDIAI
Sbjct: 63  NVQSITKESIVASEV----TEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAI 122

Query: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180
           AFSGAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEV
Sbjct: 123 AFSGAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEV 182

Query: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 240
           Q LVRNKGLFSFYEDGHQECCR+RKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV
Sbjct: 183 QALVRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 242

Query: 241 DPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300
           DPVFEGLDGGVGSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VL
Sbjct: 243 DPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVL 302

Query: 301 PGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLN 360
           PGQHEREGRWWWED+KAKECGLHKGN+K+      N   +VNG+  V DIF S N+VNL+
Sbjct: 303 PGQHEREGRWWWEDAKAKECGLHKGNIKEN--TNGNATANVNGTASVADIFNSENVVNLS 362

Query: 361 RTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQK 420
           R GIENL +LENRKE W+VVLYAPWC FCQAME S+ ELA+KL GSGVKV KFR+DG+QK
Sbjct: 363 RQGIENLMKLENRKEAWIVVLYAPWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQK 422

Query: 421 EFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           +FAK+ELQLGSFPTIL FPK+SS+ IKYPSEKRDVDSL +F+N +R
Sbjct: 423 DFAKKELQLGSFPTILVFPKNSSRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Lcy06g005860 vs. ExPASy Swiss-Prot
Match: P92981 (5'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR2 PE=1 SV=2)

HSP 1 Score: 681.4 bits (1757), Expect = 7.2e-195
Identity = 345/469 (73.56%), Postives = 393/469 (83.80%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           MAL A +SSS++IS S+     ASSE+KA +I S +L +R H        S R  S+KP+
Sbjct: 1   MAL-AVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPL 60

Query: 61  NAQS-KRNDSLVPLAANTLNAEVADK-AETVDYDGLAKDLENASPLEIMDKALEKFGNDI 120
           NA+S  R++S V  A+  +  EV +K  E  D++ LAK LE+ASPLEIMDKALE+FG+ I
Sbjct: 61  NAESHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQI 120

Query: 121 AIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAV 180
           AIAFSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAV
Sbjct: 121 AIAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAV 180

Query: 181 EVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVV 240
           EVQ LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP+V
Sbjct: 181 EVQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIV 240

Query: 241 QVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRP 300
           QVDPVFEGLDGGVGSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRP
Sbjct: 241 QVDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRP 300

Query: 301 VLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSP-PVNDIFISPNLV 360
           VLPGQHEREGRWWWED+KAKECGLHKGN+K+ED       G  +  P  V +IF S N+V
Sbjct: 301 VLPGQHEREGRWWWEDAKAKECGLHKGNIKEED-------GAADSKPAAVQEIFESNNVV 360

Query: 361 NLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDG 420
            L++ G+ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKLAG GVKV KFR+DG
Sbjct: 361 ALSKGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADG 420

Query: 421 EQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           EQKEFAK+ELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 421 EQKEFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Lcy06g005860 vs. ExPASy Swiss-Prot
Match: Q6Z4A7 (Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=APR1 PE=2 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 5.0e-180
Identity = 320/474 (67.51%), Postives = 366/474 (77.22%), Query Frame = 0

Query: 11  SSISASNLQHFAASSEAKAPRIGSF--QLLNRPHFVSAGVNYS-------LRGC--SVKP 70
           +S +AS   H  A  + KA RIG+   Q+   P   +A            LR    + +P
Sbjct: 2   ASATASISSHSVALRDLKAARIGAVKQQVAAAPAAGTAAARAQRARAVRPLRAAEPARQP 61

Query: 71  INAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIA 130
           ++A +    +  P+A +   A V   A  VDY+ LA++L+ ASPLEIMD+AL  FG+DIA
Sbjct: 62  VSASAAAAPAAAPVAEDAAAAAVDAPAPAVDYEALAQELQGASPLEIMDRALAMFGSDIA 121

Query: 131 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 190
           IAFSGAEDVALIEYA+LTGRPFRVFSLDTGRLN ETYQ FD VEKHYGI IEYMFPDA E
Sbjct: 122 IAFSGAEDVALIEYAKLTGRPFRVFSLDTGRLNPETYQLFDKVEKHYGIRIEYMFPDAGE 181

Query: 191 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQ 250
           VQ LVR KGLFSFYEDGHQECCR RKVRPLRRAL+GLRAWITGQRKDQSPGTR+ IPVVQ
Sbjct: 182 VQALVRAKGLFSFYEDGHQECCRARKVRPLRRALRGLRAWITGQRKDQSPGTRAAIPVVQ 241

Query: 251 VDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 310
           VDP FEGL GG GSL+KWNPVANVDGKD+W FLR M+VPVN+LH+QGYVSIGCEPCTRPV
Sbjct: 242 VDPSFEGLAGGAGSLVKWNPVANVDGKDVWTFLRAMDVPVNALHAQGYVSIGCEPCTRPV 301

Query: 311 LPGQHEREGRWWWEDSKAKECGLHKGNLKQED-----VAQLNGNGHVNGSPPVNDIFISP 370
           LPGQHEREGRWWWED+KAKECGLHKGN+  +       A   G  + NGS    DIF S 
Sbjct: 302 LPGQHEREGRWWWEDAKAKECGLHKGNIDDQGGAAAAAAHKAGGANGNGSAGAPDIFESS 361

Query: 371 NLVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKL--AGSGVKVGK 430
            +V+L R G+ENL RLE+R EPWLVVLYAPWC FCQAME SY+ELAE+L  AG GVKVGK
Sbjct: 362 GVVSLTRAGVENLLRLESRAEPWLVVLYAPWCPFCQAMEASYLELAERLGGAGGGVKVGK 421

Query: 431 FRSDGEQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           FR+DGEQK FA++ELQL SFPTIL FP  +++ IKYPSEKRDVDSL+AFVN+LR
Sbjct: 422 FRADGEQKAFAQQELQLQSFPTILLFPSRTARPIKYPSEKRDVDSLLAFVNSLR 475

BLAST of Lcy06g005860 vs. ExPASy Swiss-Prot
Match: B7VBC3 (Adenosine 5'-phosphosulfate reductase OS=Pseudomonas aeruginosa (strain LESB58) OX=557722 GN=cysH PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.9e-78
Identity = 130/242 (53.72%), Postives = 177/242 (73.14%), Query Frame = 0

Query: 86  AETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYARLTGRPFRVFS 145
           ++  D   LA  L + SP +I+  A E FG+++ I+FSGAEDV L++ A    R  +VFS
Sbjct: 25  SQPFDLPALASSLADKSPQDILKAAFEHFGDELWISFSGAEDVVLVDMAWKLNRNVKVFS 84

Query: 146 LDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRK 205
           LDTGRL+ ETY+F D V +HYGI I+ + PD   ++ LV+ KGLFSFY DGH ECC +RK
Sbjct: 85  LDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRK 144

Query: 206 VRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGSLIKWNPVANVDG 265
           + PL+R L G+RAW TGQR+DQSPGTRS++ V+++D  F   +     L K+NP++++  
Sbjct: 145 IEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPE---KPLYKFNPLSSMTS 204

Query: 266 KDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDSKAKECGLHKG 325
           +++W ++R + +P NSLH +GY+SIGCEPCTRPVLP QHEREGRWWWE++  KECGLH G
Sbjct: 205 EEVWGYIRMLELPYNSLHERGYISIGCEPCTRPVLPNQHEREGRWWWEEATHKECGLHAG 263

Query: 326 NL 328
           NL
Sbjct: 265 NL 263

BLAST of Lcy06g005860 vs. ExPASy TrEMBL
Match: A0A6J1E7I7 (5'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431516 PE=4 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.2e-242
Identity = 413/453 (91.17%), Postives = 428/453 (94.48%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS QLLN PH  S+GVNY  RGC +KPIN+QS+RN+  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSQSQRNNLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NT+ +EV +KAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+PPVNDIFIS NLVNLNRTGIENLARL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPPVNDIFISQNLVNLNRTGIENLARLGNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKSELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Lcy06g005860 vs. ExPASy TrEMBL
Match: A0A6J1KM85 (5'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495445 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.8e-241
Identity = 411/453 (90.73%), Postives = 427/453 (94.26%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS Q LN PH +S+GVNY  RGC VKPIN+QS+RND  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQPLNWPHVLSSGVNYLRRGCRVKPINSQSQRNDLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NTL +EV +KAETVDYDGLAKD E+ASPLEIMD+ALEKFGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTLASEVVEKAETVDYDGLAKDFEDASPLEIMDQALEKFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNP+ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPLANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+P VNDIFIS NLVNLNRTGIENL+RL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPLVNDIFISQNLVNLNRTGIENLSRLVNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSL+AFVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLLAFVNALR 457

BLAST of Lcy06g005860 vs. ExPASy TrEMBL
Match: A0A5A7UP26 (5'-adenylylsulfate reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005680 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.9e-240
Identity = 418/469 (89.13%), Postives = 441/469 (94.03%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAA-SSEAKAPRIGSFQLLNRPHFVSAGVNYS-LRGCS-V 60
           MAL  +SSS S  S+SN  HFAA SSE K PRIGS QLL+RPH +S+GVNYS + GCS V
Sbjct: 1   MALSVSSSSIS--SSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVV 60

Query: 61  KPINAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGND 120
           KP+NAQSKR+DSLVP  ANTL AEVA+K E VDYDGLA+DLENASPLEIMD+ALEKFG+D
Sbjct: 61  KPMNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDD 120

Query: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180
           IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA
Sbjct: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180

Query: 181 VEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPV 240
           VEVQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRS++PV
Sbjct: 181 VEVQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPV 240

Query: 241 VQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300
           VQVDPVFEGLDGG+GSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR
Sbjct: 241 VQVDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300

Query: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLV 360
           PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQ DV QLNGNG  N  PPVNDIFIS +LV
Sbjct: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNG--NEIPPVNDIFISQDLV 360

Query: 361 NLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDG 420
           NLNRTG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFR+DG
Sbjct: 361 NLNRTGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADG 420

Query: 421 EQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           EQK+FAK+ELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 EQKQFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Lcy06g005860 vs. ExPASy TrEMBL
Match: E5GC59 (Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.9e-240
Identity = 418/469 (89.13%), Postives = 441/469 (94.03%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAA-SSEAKAPRIGSFQLLNRPHFVSAGVNYS-LRGCS-V 60
           MAL  +SSS S  S+SN  HFAA SSE K PRIGS QLL+RPH +S+GVNYS + GCS V
Sbjct: 1   MALSVSSSSIS--SSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVV 60

Query: 61  KPINAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGND 120
           KP+NAQSKR+DSLVP  ANTL AEVA+K E VDYDGLA+DLENASPLEIMD+ALEKFG+D
Sbjct: 61  KPMNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDD 120

Query: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180
           IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA
Sbjct: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180

Query: 181 VEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPV 240
           VEVQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRS++PV
Sbjct: 181 VEVQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPV 240

Query: 241 VQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300
           VQVDPVFEGLDGG+GSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR
Sbjct: 241 VQVDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300

Query: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLV 360
           PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQ DV QLNGNG  N  PPVNDIFIS +LV
Sbjct: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNG--NEIPPVNDIFISQDLV 360

Query: 361 NLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDG 420
           NLNRTG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFR+DG
Sbjct: 361 NLNRTGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADG 420

Query: 421 EQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           EQK+FAK+ELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 EQKQFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Lcy06g005860 vs. ExPASy TrEMBL
Match: A0A1S3B2C8 (5'-adenylylsulfate reductase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485224 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.9e-240
Identity = 418/469 (89.13%), Postives = 441/469 (94.03%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAA-SSEAKAPRIGSFQLLNRPHFVSAGVNYS-LRGCS-V 60
           MAL  +SSS S  S+SN  HFAA SSE K PRIGS QLL+RPH +S+GVNYS + GCS V
Sbjct: 1   MALSVSSSSIS--SSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVV 60

Query: 61  KPINAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGND 120
           KP+NAQSKR+DSLVP  ANTL AEVA+K E VDYDGLA+DLENASPLEIMD+ALEKFG+D
Sbjct: 61  KPMNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDD 120

Query: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180
           IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA
Sbjct: 121 IAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDA 180

Query: 181 VEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPV 240
           VEVQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRS++PV
Sbjct: 181 VEVQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPV 240

Query: 241 VQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300
           VQVDPVFEGLDGG+GSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR
Sbjct: 241 VQVDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTR 300

Query: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLV 360
           PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQ DV QLNGNG  N  PPVNDIFIS +LV
Sbjct: 301 PVLPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNG--NEIPPVNDIFISQDLV 360

Query: 361 NLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDG 420
           NLNRTG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFR+DG
Sbjct: 361 NLNRTGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADG 420

Query: 421 EQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           EQK+FAK+ELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 EQKQFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Lcy06g005860 vs. NCBI nr
Match: XP_038895661.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 854.4 bits (2206), Expect = 4.7e-244
Identity = 420/461 (91.11%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 8   SSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRN 67
           SS SSISASN QHF ASSEAK PRIGS QL+NRPH +S+GVNYS RGCS+KP+NAQS RN
Sbjct: 6   SSHSSISASNFQHFPASSEAKVPRIGSVQLVNRPHVLSSGVNYSQRGCSMKPMNAQSNRN 65

Query: 68  DSLVPL--AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGA 127
            SLV L  AANTL AEVA++AE V+YDGLAK LENASPLEIMD+ALEKFG+DIAIAFSGA
Sbjct: 66  VSLVSLAVAANTLTAEVAERAEIVNYDGLAKHLENASPLEIMDQALEKFGDDIAIAFSGA 125

Query: 128 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVR 187
           EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVR
Sbjct: 126 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVR 185

Query: 188 NKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFE 247
           NKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFE
Sbjct: 186 NKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFE 245

Query: 248 GLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHE 307
           GLDGG+GSLIKWNPVANVDGKD+WNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHE
Sbjct: 246 GLDGGIGSLIKWNPVANVDGKDVWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHE 305

Query: 308 REGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIE 367
           REGRWWWEDSKAKECGLHKGNLKQ DV QLNGN   NG+PPVNDIFIS NLVNLNRTGIE
Sbjct: 306 REGRWWWEDSKAKECGLHKGNLKQGDVPQLNGN--ENGTPPVNDIFISQNLVNLNRTGIE 365

Query: 368 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKR 427
           NLARL+NRKEPWLVVLYAPWCQFCQ MEGSYVELAEKL GSGVKVGKFR+DGEQK+FAK+
Sbjct: 366 NLARLDNRKEPWLVVLYAPWCQFCQGMEGSYVELAEKLGGSGVKVGKFRADGEQKQFAKQ 425

Query: 428 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           ELQLGSFPTIL FPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 426 ELQLGSFPTILLFPKHSSKAIKYPSEKRDVDSLMAFVNALR 464

BLAST of Lcy06g005860 vs. NCBI nr
Match: XP_022923952.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita moschata] >KAG7019694.1 5'-adenylylsulfate reductase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 848.6 bits (2191), Expect = 2.6e-242
Identity = 413/453 (91.17%), Postives = 428/453 (94.48%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS QLLN PH  S+GVNY  RGC +KPIN+QS+RN+  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSQSQRNNLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NT+ +EV +KAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+PPVNDIFIS NLVNLNRTGIENLARL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPPVNDIFISQNLVNLNRTGIENLARLGNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKSELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Lcy06g005860 vs. NCBI nr
Match: KAG6584094.1 (5'-adenylylsulfate reductase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.0 bits (2187), Expect = 7.5e-242
Identity = 412/453 (90.95%), Postives = 428/453 (94.48%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS QLLN PH  S+GVNY  RGC +KPIN++S+RN+  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSRSQRNNLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NT+ +EV +KAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+PPVNDIFIS NLVNLNRTGIENLARL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPPVNDIFISQNLVNLNRTGIENLARLGNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKSELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Lcy06g005860 vs. NCBI nr
Match: XP_023520098.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.7 bits (2181), Expect = 3.7e-241
Identity = 412/453 (90.95%), Postives = 427/453 (94.26%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS QLLN PH +S+GVNY  RGC +KPIN+QS+RN+  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NTL +EV +KAETVDYDGLAK LENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+P VNDIFIS NLVNLNRTGIENLARL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPLVNDIFISQNLVNLNRTGIENLARLGNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSLM FVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 457

BLAST of Lcy06g005860 vs. NCBI nr
Match: XP_023001259.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 844.7 bits (2181), Expect = 3.7e-241
Identity = 411/453 (90.73%), Postives = 427/453 (94.26%), Query Frame = 0

Query: 14  SASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPINAQSKRNDSLVPL 73
           S SNL HFA SS+AKAPRIGS Q LN PH +S+GVNY  RGC VKPIN+QS+RND  VPL
Sbjct: 7   SVSNLLHFAVSSDAKAPRIGSVQPLNWPHVLSSGVNYLRRGCRVKPINSQSQRNDLFVPL 66

Query: 74  AANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEY 133
           A NTL +EV +KAETVDYDGLAKD E+ASPLEIMD+ALEKFGNDIAIAFSGAEDVALIEY
Sbjct: 67  AVNTLASEVVEKAETVDYDGLAKDFEDASPLEIMDQALEKFGNDIAIAFSGAEDVALIEY 126

Query: 134 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 193
           ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY
Sbjct: 127 ARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFY 186

Query: 194 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGS 253
           EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GS
Sbjct: 187 EDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGS 246

Query: 254 LIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWE 313
           LIKWNP+ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRWWWE
Sbjct: 247 LIKWNPLANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWE 306

Query: 314 DSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLNRTGIENLARLENR 373
           DSKAKECGLHKGNLKQ D+ QLNGNG  NG+P VNDIFIS NLVNLNRTGIENL+RL NR
Sbjct: 307 DSKAKECGLHKGNLKQGDIPQLNGNG--NGTPLVNDIFISQNLVNLNRTGIENLSRLVNR 366

Query: 374 KEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQKEFAKRELQLGSFP 433
           KEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFR+DGEQKEFAK ELQLGSFP
Sbjct: 367 KEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFP 426

Query: 434 TILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           TILFFPKHSSKAIKYPSEKRDVDSL+AFVNALR
Sbjct: 427 TILFFPKHSSKAIKYPSEKRDVDSLLAFVNALR 457

BLAST of Lcy06g005860 vs. TAIR 10
Match: AT4G04610.1 (APS reductase 1 )

HSP 1 Score: 702.2 bits (1811), Expect = 2.8e-202
Identity = 351/471 (74.52%), Postives = 400/471 (84.93%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYS-LRGCSVKP 60
           M++  +SSSSS I  S    F  S E K  +IGS +LL+R H     +N S  R  SVKP
Sbjct: 3   MSVNVSSSSSSGIINS---RFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKP 62

Query: 61  INAQSKRNDSLVPLAANTLNAEVADKAETV---DYDGLAKDLENASPLEIMDKALEKFGN 120
           +NA+ K  DS++PLAA T+ AE+A++ E V   D++ LAK LENASPLEIMDKALEK+GN
Sbjct: 63  LNAEPKTKDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGN 122

Query: 121 DIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPD 180
           DIAIAFSGAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD
Sbjct: 123 DIAIAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPD 182

Query: 181 AVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIP 240
           +VEVQGLVR+KGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP
Sbjct: 183 SVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIP 242

Query: 241 VVQVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCT 300
           VVQVDPVFEGLDGGVGSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT
Sbjct: 243 VVQVDPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCT 302

Query: 301 RPVLPGQHEREGRWWWEDSKAKECGLHKGNLKQ-EDVAQLNGNGHVNGSPPVNDIFISPN 360
           + VLPGQHEREGRWWWED+KAKECGLHKGN+K+  D A++NG         V DIF S N
Sbjct: 303 KAVLPGQHEREGRWWWEDAKAKECGLHKGNVKENSDDAKVNG----ESKSAVADIFKSEN 362

Query: 361 LVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRS 420
           LV L+R GIENL +LENRKEPW+VVLYAPWC FCQAME SY ELA+KLAGSG+KV KFR+
Sbjct: 363 LVTLSRQGIENLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRA 422

Query: 421 DGEQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           DG+QKEFAK+ELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL +F+N +R
Sbjct: 423 DGDQKEFAKQELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Lcy06g005860 vs. TAIR 10
Match: AT4G21990.1 (APS reductase 3 )

HSP 1 Score: 687.6 bits (1773), Expect = 7.1e-198
Identity = 341/466 (73.18%), Postives = 389/466 (83.48%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           +A+  +SSSSS+IS+S+      SS+ K  +IGS +LLNR +  +A ++ S +  SVK +
Sbjct: 3   LAINVSSSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKAL 62

Query: 61  NAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAI 120
           N QS   +S+V         E  D  E  D++ LAK LENASPLEIMDKALEKFGNDIAI
Sbjct: 63  NVQSITKESIVASEV----TEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAI 122

Query: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180
           AFSGAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEV
Sbjct: 123 AFSGAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEV 182

Query: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 240
           Q LVRNKGLFSFYEDGHQECCR+RKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV
Sbjct: 183 QALVRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 242

Query: 241 DPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300
           DPVFEGLDGGVGSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VL
Sbjct: 243 DPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVL 302

Query: 301 PGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSPPVNDIFISPNLVNLN 360
           PGQHEREGRWWWED+KAKECGLHKGN+K+      N   +VNG+  V DIF S N+VNL+
Sbjct: 303 PGQHEREGRWWWEDAKAKECGLHKGNIKEN--TNGNATANVNGTASVADIFNSENVVNLS 362

Query: 361 RTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGEQK 420
           R GIENL +LENRKE W+VVLYAPWC FCQAME S+ ELA+KL GSGVKV KFR+DG+QK
Sbjct: 363 RQGIENLMKLENRKEAWIVVLYAPWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQK 422

Query: 421 EFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           +FAK+ELQLGSFPTIL FPK+SS+ IKYPSEKRDVDSL +F+N +R
Sbjct: 423 DFAKKELQLGSFPTILVFPKNSSRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Lcy06g005860 vs. TAIR 10
Match: AT1G62180.1 (5'adenylylphosphosulfate reductase 2 )

HSP 1 Score: 681.4 bits (1757), Expect = 5.1e-196
Identity = 345/469 (73.56%), Postives = 393/469 (83.80%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           MAL A +SSS++IS S+     ASSE+KA +I S +L +R H        S R  S+KP+
Sbjct: 1   MAL-AVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPL 60

Query: 61  NAQS-KRNDSLVPLAANTLNAEVADK-AETVDYDGLAKDLENASPLEIMDKALEKFGNDI 120
           NA+S  R++S V  A+  +  EV +K  E  D++ LAK LE+ASPLEIMDKALE+FG+ I
Sbjct: 61  NAESHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQI 120

Query: 121 AIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAV 180
           AIAFSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAV
Sbjct: 121 AIAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAV 180

Query: 181 EVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVV 240
           EVQ LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP+V
Sbjct: 181 EVQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIV 240

Query: 241 QVDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRP 300
           QVDPVFEGLDGGVGSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRP
Sbjct: 241 QVDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRP 300

Query: 301 VLPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSP-PVNDIFISPNLV 360
           VLPGQHEREGRWWWED+KAKECGLHKGN+K+ED       G  +  P  V +IF S N+V
Sbjct: 301 VLPGQHEREGRWWWEDAKAKECGLHKGNIKEED-------GAADSKPAAVQEIFESNNVV 360

Query: 361 NLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDG 420
            L++ G+ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKLAG GVKV KFR+DG
Sbjct: 361 ALSKGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADG 420

Query: 421 EQKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           EQKEFAK+ELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 421 EQKEFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Lcy06g005860 vs. TAIR 10
Match: AT1G62180.2 (5'adenylylphosphosulfate reductase 2 )

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-194
Identity = 343/468 (73.29%), Postives = 390/468 (83.33%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           MAL A +SSS++IS S+     ASSE+KA +I S +L +R H        S R  S+KP+
Sbjct: 1   MAL-AVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPL 60

Query: 61  NAQS-KRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           NA+S  R++S V  A+  +  EV       D++ LAK LE+ASPLEIMDKALE+FG+ IA
Sbjct: 61  NAESHSRSESWVTRASTLIAPEVE------DFEQLAKKLEDASPLEIMDKALERFGDQIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQ 240
           VQ LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP+VQ
Sbjct: 181 VQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQ 240

Query: 241 VDPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDPVFEGLDGGVGSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPV 300

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEDVAQLNGNGHVNGSP-PVNDIFISPNLVN 360
           LPGQHEREGRWWWED+KAKECGLHKGN+K+ED       G  +  P  V +IF S N+V 
Sbjct: 301 LPGQHEREGRWWWEDAKAKECGLHKGNIKEED-------GAADSKPAAVQEIFESNNVVA 360

Query: 361 LNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRSDGE 420
           L++ G+ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKLAG GVKV KFR+DGE
Sbjct: 361 LSKGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGE 420

Query: 421 QKEFAKRELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 467
           QKEFAK+ELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 421 QKEFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 447

BLAST of Lcy06g005860 vs. TAIR 10
Match: AT4G21990.2 (APS reductase 3 )

HSP 1 Score: 459.9 bits (1182), Expect = 2.4e-129
Identity = 231/310 (74.52%), Postives = 259/310 (83.55%), Query Frame = 0

Query: 1   MALPAASSSSSSISASNLQHFAASSEAKAPRIGSFQLLNRPHFVSAGVNYSLRGCSVKPI 60
           +A+  +SSSSS+IS+S+      SS+ K  +IGS +LLNR +  +A ++ S +  SVK +
Sbjct: 38  LAINVSSSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKAL 97

Query: 61  NAQSKRNDSLVPLAANTLNAEVADKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAI 120
           N QS   +S+V         E  D  E  D++ LAK LENASPLEIMDKALEKFGNDIAI
Sbjct: 98  NVQSITKESIVASEV----TEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAI 157

Query: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180
           AFSGAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEV
Sbjct: 158 AFSGAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEV 217

Query: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 240
           Q LVRNKGLFSFYEDGHQECCR+RKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV
Sbjct: 218 QALVRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 277

Query: 241 DPVFEGLDGGVGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300
           DPVFEGLDGGVGSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VL
Sbjct: 278 DPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVL 337

Query: 301 PGQHEREGRW 311
           PGQHEREGRW
Sbjct: 338 PGQHEREGRW 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929793.9e-20174.525'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P929801.0e-19673.185'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P929817.2e-19573.565'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6Z4A75.0e-18067.51Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. ja... [more]
B7VBC31.9e-7853.72Adenosine 5'-phosphosulfate reductase OS=Pseudomonas aeruginosa (strain LESB58) ... [more]
Match NameE-valueIdentityDescription
A0A6J1E7I71.2e-24291.175'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1KM851.8e-24190.735'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 G... [more]
A0A5A7UP269.9e-24089.135'-adenylylsulfate reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
E5GC599.9e-24089.13Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3B2C89.9e-24089.135'-adenylylsulfate reductase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
XP_038895661.14.7e-24491.115'-adenylylsulfate reductase 3, chloroplastic-like [Benincasa hispida][more]
XP_022923952.12.6e-24291.175'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita moschata] >KAG7019... [more]
KAG6584094.17.5e-24290.955'-adenylylsulfate reductase 3, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_023520098.13.7e-24190.955'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001259.13.7e-24190.735'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G04610.12.8e-20274.52APS reductase 1 [more]
AT4G21990.17.1e-19873.18APS reductase 3 [more]
AT1G62180.15.1e-19673.565'adenylylphosphosulfate reductase 2 [more]
AT1G62180.22.1e-19473.295'adenylylphosphosulfate reductase 2 [more]
AT4G21990.22.4e-12974.52APS reductase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 89..313
e-value: 4.0E-71
score: 241.0
IPR004508Thioredoxin-independent 5'-adenylylsulphate reductaseTIGRFAMTIGR00424TIGR00424coord: 1..466
e-value: 9.0E-268
score: 886.3
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 352..464
e-value: 1.1E-18
score: 69.2
NoneNo IPR availablePANTHERPTHR464825'-ADENYLYLSULFATE REDUCTASE 3, CHLOROPLASTICcoord: 22..466
NoneNo IPR availablePANTHERPTHR46482:SF85'-ADENYLYLSULFATE REDUCTASE 1, CHLOROPLASTICcoord: 22..466
NoneNo IPR availableCDDcd02993PDI_a_APS_reductasecoord: 354..462
e-value: 7.18124E-63
score: 197.675
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 91..286
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 369..463
e-value: 1.7E-11
score: 44.0
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 323..466
score: 11.753935
IPR002500Phosphoadenosine phosphosulphate reductasePFAMPF01507PAPS_reductcoord: 118..298
e-value: 4.3E-44
score: 150.6
IPR002500Phosphoadenosine phosphosulphate reductaseCDDcd01713PAPS_reductasecoord: 117..291
e-value: 3.39745E-54
score: 177.212
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 355..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g005860.1Lcy06g005860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0019419 sulfate reduction
molecular_function GO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016671 oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor