Lcy06g004960 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g004960
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionglucuronoxylan 4-O-methyltransferase 1
LocationChr06: 4609720 .. 4610670 (-)
RNA-Seq ExpressionLcy06g004960
SyntenyLcy06g004960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCCAGAAGTAGCGGACTGCCACTCACTTGTGTCATCAGAGTCCTCGTTTTCTCCCCTCTCTGTGTCCCCCTCACAGCAGCCATACCCATCAGCAAGCTTCAAAAACAGTAGGGGAAGCAAAAACATGAATTTTACGAGAAACAAAATCATACCTGTTCTCTTGTTGGTTCTGTCTAGTCTCTCCATTGTCAGACTTGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGGAATTGCATTGGCTCCAAAGCTTCAACACCCAGCATCAGAGCTCTCAAAAAATCATGGTAGAAACTCTGCAAAAGAAGCCGCCATCTCAGAGAAGGAATTCAAGCTTCTTTCAGATCTCGTTGCCAAAAAAGCACCCTGCAATCTTCTGATCTTTGGGTTAGAATCACAGTACCTTAACCTCTCGGCAATCAATGATGGGGGAAATAATGTTTTTCTAGAGGATGATCCCTACAAACTGAGCACATTCAAAATCAACTCAAATCACACACGAATGTACAAAGTTGATTACAAGCTTCATGCAGAAGAAGCTTACCAGCTGTTAAAGCTTGCAAGACGAAACAAAACATGCGCACCAAACGCAGGGCTGATAAATTCATCTAAGTGCCAACTTGCCTTGACAGATCTTCCAAGAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGAGGAGATAGTCCTCAGGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTGTCATAGCAAGAGAAGGGAACACCACCGATGTAGTCGTACACGATATAGATCGCATAATCGAAAAGTGGTATTCCTGGGAGTTTCTTTGTGATGAAAACTTGATTTCCTCCAAAGGAAAACTCTGGATTTTCAGGATTAGAAATGAGATAAATTCTACCAGATTCTGTCCTGCGAAAGACATTGTGATCCAGTAA

mRNA sequence

ATGCCTCCAGAAGTAGCGGACTGCCACTCACTTGTGTCATCAGAGTCCTCGTTTTCTCCCCTCTCTGTGTCCCCCTCACAGCAGCCATACCCATCAGCAAGCTTCAAAAACAGTAGGGGAAGCAAAAACATGAATTTTACGAGAAACAAAATCATACCTGTTCTCTTGTTGGTTCTGTCTAGTCTCTCCATTGTCAGACTTGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGGAATTGCATTGGCTCCAAAGCTTCAACACCCAGCATCAGAGCTCTCAAAAAATCATGGTAGAAACTCTGCAAAAGAAGCCGCCATCTCAGAGAAGGAATTCAAGCTTCTTTCAGATCTCGTTGCCAAAAAAGCACCCTGCAATCTTCTGATCTTTGGGTTAGAATCACAGTACCTTAACCTCTCGGCAATCAATGATGGGGGAAATAATGTTTTTCTAGAGGATGATCCCTACAAACTGAGCACATTCAAAATCAACTCAAATCACACACGAATGTACAAAGTTGATTACAAGCTTCATGCAGAAGAAGCTTACCAGCTGTTAAAGCTTGCAAGACGAAACAAAACATGCGCACCAAACGCAGGGCTGATAAATTCATCTAAGTGCCAACTTGCCTTGACAGATCTTCCAAGAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGAGGAGATAGTCCTCAGGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTGTCATAGCAAGAGAAGGGAACACCACCGATGTAGTCGTACACGATATAGATCGCATAATCGAAAAGTGGTATTCCTGGGAGTTTCTTTGTGATGAAAACTTGATTTCCTCCAAAGGAAAACTCTGGATTTTCAGGATTAGAAATGAGATAAATTCTACCAGATTCTGTCCTGCGAAAGACATTGTGATCCAGTAA

Coding sequence (CDS)

ATGCCTCCAGAAGTAGCGGACTGCCACTCACTTGTGTCATCAGAGTCCTCGTTTTCTCCCCTCTCTGTGTCCCCCTCACAGCAGCCATACCCATCAGCAAGCTTCAAAAACAGTAGGGGAAGCAAAAACATGAATTTTACGAGAAACAAAATCATACCTGTTCTCTTGTTGGTTCTGTCTAGTCTCTCCATTGTCAGACTTGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGGAATTGCATTGGCTCCAAAGCTTCAACACCCAGCATCAGAGCTCTCAAAAAATCATGGTAGAAACTCTGCAAAAGAAGCCGCCATCTCAGAGAAGGAATTCAAGCTTCTTTCAGATCTCGTTGCCAAAAAAGCACCCTGCAATCTTCTGATCTTTGGGTTAGAATCACAGTACCTTAACCTCTCGGCAATCAATGATGGGGGAAATAATGTTTTTCTAGAGGATGATCCCTACAAACTGAGCACATTCAAAATCAACTCAAATCACACACGAATGTACAAAGTTGATTACAAGCTTCATGCAGAAGAAGCTTACCAGCTGTTAAAGCTTGCAAGACGAAACAAAACATGCGCACCAAACGCAGGGCTGATAAATTCATCTAAGTGCCAACTTGCCTTGACAGATCTTCCAAGAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGAGGAGATAGTCCTCAGGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTGTCATAGCAAGAGAAGGGAACACCACCGATGTAGTCGTACACGATATAGATCGCATAATCGAAAAGTGGTATTCCTGGGAGTTTCTTTGTGATGAAAACTTGATTTCCTCCAAAGGAAAACTCTGGATTTTCAGGATTAGAAATGAGATAAATTCTACCAGATTCTGTCCTGCGAAAGACATTGTGATCCAGTAA

Protein sequence

MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLSSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKLHAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRNEINSTRFCPAKDIVIQ
Homology
BLAST of Lcy06g004960 vs. ExPASy Swiss-Prot
Match: Q9FH92 (Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.8e-33
Identity = 97/258 (37.60%), Postives = 142/258 (55.04%), Query Frame = 0

Query: 60  SSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLS 119
           +S ++   V  A+TT+  S L   A+   L + +      H         +S  E K +S
Sbjct: 57  NSATVSSAVNSAVTTATISQLPTTAINAMLHYASRSNDSYH---------MSYGEMKSIS 116

Query: 120 DLVAK-KAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDY 179
           D++ +   PCNLL+FGL  + L   ++N  G  VF+E++ Y  + F+       ++ V Y
Sbjct: 117 DVLRRCSPPCNLLVFGLTHETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIEVFDVQY 176

Query: 180 KLHAEEAYQL---LKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPR 239
              A EA +L   +K A RN+ C P   L+  S C+L L DLP  VY+V WDV++VDGPR
Sbjct: 177 TTKAREARELVSAVKEAARNE-CRPVQNLL-FSDCKLGLNDLPNHVYDVDWDVILVDGPR 236

Query: 240 GDSPQAPGRMATIYSAAVIARE---GN-TTDVVVHDIDRIIEKWYSWEFLCDENLISSKG 299
           GD    PGRM++I++AAV+AR    GN  T V VHD  R +E+    EFLC ENL+ S  
Sbjct: 237 GDGGDVPGRMSSIFTAAVLARSKKGGNPKTHVFVHDYYRDVERLCGDEFLCRENLVESND 296

Query: 300 KLWIFRI-RNEINSTRFC 309
            L  + + + + NST+FC
Sbjct: 297 LLAHYVLEKMDKNSTQFC 303

BLAST of Lcy06g004960 vs. ExPASy Swiss-Prot
Match: Q9SNE5 (Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.0e-31
Identity = 99/282 (35.11%), Postives = 149/282 (52.84%), Query Frame = 0

Query: 55  LLLVLSSLSIVRLVRIALT--------TSYTSPLLGIALAPKLQHPASELSKNHGRNSAK 114
           LL  +S  +IV L+ +  T        TS  + +  +         +S +S ++   SA 
Sbjct: 30  LLAFVSFFTIVFLLTLLYTRDTIPSKNTSVAAAVAAVVTGGSTPSASSPISNSNLPTSAI 89

Query: 115 EAAI------------SEKEFKLLSDLVAKKA-PCNLLIFGLESQYLNLSAINDGGNNVF 174
            A +            S  E K +SD++ + A PCNLL+FGL  + L   ++N  G  VF
Sbjct: 90  NALLHYASRSNDSFHMSYGEMKSISDVLRRCAPPCNLLVFGLTHETLLWKSLNHNGRTVF 149

Query: 175 LEDDPYKLSTFKINSNHTRMYKVDYKLHAEEAYQLLKLARR--NKTCAPNAGLINSSKCQ 234
           +E++ Y  + F+       ++ V Y   A EA +L+  A+      C P   L+  S C+
Sbjct: 150 IEENRYYAAYFEEIHPEIDVFDVQYTTKAHEAGELVTAAKEAAGNECRPVQNLL-FSDCK 209

Query: 235 LALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMATIYSAAVIAR--EGNT--TDVVVHD 294
           L L DLP  VY+V WDV+ VDGPRGD+ + PGRM++I++AAV+AR  +G T  T V VHD
Sbjct: 210 LGLNDLPNHVYDVDWDVIFVDGPRGDAHEGPGRMSSIFTAAVLARSKKGGTPKTHVFVHD 269

Query: 295 IDRIIEKWYSWEFLCDENLISSKGKLWIFRI-RNEINSTRFC 309
             R +E+    EFLC ENL+ S   L  + + + + NST+FC
Sbjct: 270 YYRDVERLCGDEFLCRENLVESNDLLAHYVLDKMDKNSTKFC 310

BLAST of Lcy06g004960 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.9e-30
Identity = 93/276 (33.70%), Postives = 143/276 (51.81%), Query Frame = 0

Query: 49  NKIIPVLLLVLSSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKN------HGR 108
           +K+  +L+ +L++L ++ +VR  LT+S          + +     ++L ++      H  
Sbjct: 9   HKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSGACNKLPRSLAQALIHYS 68

Query: 109 NSAKEAAISEKEFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLS 168
            S      + KE  + S ++ KK+PCN L+FGL    L  S++N GG  VFLE+D   + 
Sbjct: 69  TSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYGGRTVFLEEDEAWIK 128

Query: 169 TFKINSNHTRMYKVDYKLHAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVY 228
             K        Y V Y     +A  L+++ +  +  A   G    S CQLAL  LP E+Y
Sbjct: 129 QIKRRFPMLESYHVTYDSKVNQADNLIEVGKGPECTA--IGDPRYSMCQLALKGLPAEIY 188

Query: 229 EVKWDVVVVDGPRGDSPQAPGRMATIYSAAVIAR---EGNTTDVVVHDIDRIIEKWYSWE 288
           E  WD+++VD P G   +APGRM  IY+A ++AR   +G  TDV VHD++R IE  +S  
Sbjct: 189 ETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVHDVNREIEDKFSKA 248

Query: 289 FLCDENLISSKGKLWIF-----RIRNEINSTR-FCP 310
           FLC+  +   +G+L  F     R  +E  S R FCP
Sbjct: 249 FLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Lcy06g004960 vs. ExPASy Swiss-Prot
Match: Q9T0F7 (Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.9e-30
Identity = 76/202 (37.62%), Postives = 115/202 (56.93%), Query Frame = 0

Query: 114 EFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRM 173
           E  +   ++ KK+PCN L+FGL    L  +++N GG  +FLE+D   + T      +   
Sbjct: 85  EVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFLEEDEAWIETVTKKFPNLES 144

Query: 174 YKVDYKLHAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDG 233
           Y V Y    +++ +L++L +R + C   +     SKC L+L   P +VYE +WDV++VD 
Sbjct: 145 YHVVYDTKVKDSNKLMEL-KRTEDCKAVSD-PRDSKCALSLKGFPADVYETQWDVIMVDA 204

Query: 234 PRGDSPQAPGRMATIYSAAVIAR---EGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSK 293
           P G   +APGRM+ IY+A ++AR   +G  TDV VHDI+R +E  +S  FLC   +   +
Sbjct: 205 PTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVFVHDINRPVEDEFSVAFLCGGYMKEQQ 264

Query: 294 GKLWIFRIRNEINS--TRFCPA 311
           G+L  F I +   S  T FCPA
Sbjct: 265 GRLRHFNIPSHRASFGTPFCPA 284

BLAST of Lcy06g004960 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.9e-30
Identity = 71/189 (37.57%), Postives = 112/189 (59.26%), Query Frame = 0

Query: 114 EFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRM 173
           E  +  +++ K AP N L+FGL    L  +++N  G  +FLE+D         +S   R 
Sbjct: 84  EISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEEDLEWFQKVTKDSPFLRA 143

Query: 174 YKVDYKLHAEEAYQLLKLARRNKTCAP-NAGLINSSKCQLALTDLPREVYEVKWDVVVVD 233
           + V Y+   ++A  LL+  +    C P  + L  + KC+LALT LP E Y+ +WD+++VD
Sbjct: 144 HHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKCKLALTGLPDEFYDTEWDLLMVD 203

Query: 234 GPRGDSPQAPGRMATIYSAAVIAR---EGNTTDVVVHDIDRIIEKWYSWEFLCDENLISS 293
            P+G   +APGRMA I+SAAV+AR   +   T V +HD++R +EK ++ EFLC +  +++
Sbjct: 204 APKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNRRVEKTFAEEFLCRKYRVNA 263

Query: 294 KGKLWIFRI 299
            G+LW F I
Sbjct: 264 AGRLWHFAI 272

BLAST of Lcy06g004960 vs. ExPASy TrEMBL
Match: A0A6J1C675 (glucuronoxylan 4-O-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111008808 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.9e-142
Identity = 262/316 (82.91%), Postives = 282/316 (89.24%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           MPP++ADCH LVSSES FSP SV   QQPYPS S KNSRGSK+MNFTR KIIPVL+LVLS
Sbjct: 1   MPPDLADCHPLVSSESLFSPPSVPSLQQPYPSLSLKNSRGSKSMNFTRKKIIPVLVLVLS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TS LL IAL PKLQ P    S N  R SA E AISEKEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSSTSSLLAIALPPKLQRPIFGFSINQSRASANEVAISEKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+AKKAPCNLL+FGLESQYLNLSAINDGG NVFLEDDPYKLSTFKI+SNHTR+YKVDYK 
Sbjct: 121 LIAKKAPCNLLVFGLESQYLNLSAINDGGKNVFLEDDPYKLSTFKISSNHTRIYKVDYKH 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
            AE+AY+LLK AR+NK CAPN+GL N SKCQLALTDLPREVYEVKWDVVVVDGP GD+PQ
Sbjct: 181 PAEKAYKLLKHARQNKACAPNSGLKNLSKCQLALTDLPREVYEVKWDVVVVDGPIGDAPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVV+HDIDR+IEKWYSWEFLCDENLISS+GKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVIHDIDRMIEKWYSWEFLCDENLISSRGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDIVIQ 317
           EINSTRFCP K I+IQ
Sbjct: 301 EINSTRFCPDKGIMIQ 316

BLAST of Lcy06g004960 vs. ExPASy TrEMBL
Match: A0A5A7UNX6 (Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001790 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.0e-139
Identity = 255/313 (81.47%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           M P V DCHSLV SE + SP S+SPSQQ  PS SF NS+GSKNM+FTRNK++PVL+L+LS
Sbjct: 1   MLPAVEDCHSLVPSE-ALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TSPLL IAL PKLQ P S  S+ H R SA EAAIS KEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+A+K PCNLL FG+ESQYLNLSA+NDGGNNVFLEDDPYKLSTFK+NSNHTRMYKVDYK 
Sbjct: 121 LIAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKF 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
           HAE+AY+LLK AR NK+CAP++GL NSSKC+LALTDLPREVYEVKWDVVVVDGPRGDSPQ
Sbjct: 181 HAEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVVVHD DR+IEKWYSWEFLCDENLISSKGKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDI 314
           E NST FCP K I
Sbjct: 301 EKNSTSFCPDKVI 312

BLAST of Lcy06g004960 vs. ExPASy TrEMBL
Match: A0A5D3BHK3 (Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004720 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.0e-139
Identity = 255/313 (81.47%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           M P V DCHSLV SE + SP S+SPSQQ  PS SF NS+GSKNM+FTRNK++PVL+L+LS
Sbjct: 1   MLPAVEDCHSLVPSE-ALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TSPLL IAL PKLQ P S  S+ H R SA EAAIS KEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+A+K PCNLL FG+ESQYLNLSA+NDGGNNVFLEDDPYKLSTFK+NSNHTRMYKVDYK 
Sbjct: 121 LIAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKF 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
           HAE+AY+LLK AR NK+CAP++GL NSSKC+LALTDLPREVYEVKWDVVVVDGPRGDSPQ
Sbjct: 181 HAEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVVVHD DR+IEKWYSWEFLCDENLISSKGKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDI 314
           E NST FCP K I
Sbjct: 301 EKNSTSFCPDKVI 312

BLAST of Lcy06g004960 vs. ExPASy TrEMBL
Match: A0A0A0LRK1 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G050520 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.8e-137
Identity = 252/312 (80.77%), Postives = 275/312 (88.14%), Query Frame = 0

Query: 5   VADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLSSLSI 64
           V DCHSLV SE S SP S+SPSQQ  PS SF NS+GSKNM+FTRNK++PVL+L+LSSLSI
Sbjct: 5   VEDCHSLVPSE-SLSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILSSLSI 64

Query: 65  VRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSDLVAK 124
           +RLVRIALTTS TSPLL IAL  KLQHP S  S+   R SA EAAIS KEFKLLS+L+A+
Sbjct: 65  LRLVRIALTTSNTSPLLAIALPSKLQHPKSGFSEKQSRTSANEAAISGKEFKLLSNLIAE 124

Query: 125 KAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKLHAEE 184
           K PCN+L FG+ESQYLNLSA+NDGGNNVFLEDD YKLSTFK+NSNHTR YKVDYK HAE+
Sbjct: 125 KTPCNVLFFGIESQYLNLSAVNDGGNNVFLEDDSYKLSTFKVNSNHTRTYKVDYKFHAEK 184

Query: 185 AYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGR 244
           AY+LLK AR NK+CAP + L NSSKC+LALTDLPREVYEVKWDVVVVDGPRGDSPQAPGR
Sbjct: 185 AYKLLKHARGNKSCAPESALRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGR 244

Query: 245 MATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRNEINS 304
           MATIYSAA+IAREGNTTDVVVHD DR+IEKWYSWEFLCDENLISSKGKLWIFRIRNE NS
Sbjct: 245 MATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRNEKNS 304

Query: 305 TRFCPAKDIVIQ 317
           T FCP K IVIQ
Sbjct: 305 TSFCPDKGIVIQ 315

BLAST of Lcy06g004960 vs. ExPASy TrEMBL
Match: A0A6J1KL98 (glucuronoxylan 4-O-methyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111495203 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.9e-128
Identity = 231/273 (84.62%), Postives = 249/273 (91.21%), Query Frame = 0

Query: 44  MNFTRNKIIPVLLLVLSSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRN 103
           M+ TRNKI+PVL+L+LSSLSI RLVRIALTTSYTSPLL IAL PKLQ P S  S+NH R 
Sbjct: 1   MSSTRNKILPVLVLILSSLSIFRLVRIALTTSYTSPLLAIALPPKLQRPISGFSRNHSRT 60

Query: 104 SAKEAAISEKEFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLST 163
           SA EA ISEKEFKLLS+L+AKK PCNLL FG+ESQYLNLSA+N GG NVFLEDDPYKLS 
Sbjct: 61  SANEATISEKEFKLLSNLIAKKVPCNLLFFGIESQYLNLSAVNPGGKNVFLEDDPYKLSI 120

Query: 164 FKINSNHTRMYKVDYKLHAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYE 223
           FK+NSNHTRMYKVDYKLHAE+AY+LLK AR NK+CAPN+GL NSS+CQLALTDLPREVYE
Sbjct: 121 FKVNSNHTRMYKVDYKLHAEKAYKLLKHARGNKSCAPNSGLRNSSRCQLALTDLPREVYE 180

Query: 224 VKWDVVVVDGPRGDSPQAPGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCD 283
           VKWDVVVVDGPRGDSP APGRMATIYSAA+IAREGNTTDVVVHDIDR+IEKWYSWEFLCD
Sbjct: 181 VKWDVVVVDGPRGDSPLAPGRMATIYSAAIIAREGNTTDVVVHDIDRMIEKWYSWEFLCD 240

Query: 284 ENLISSKGKLWIFRIRNEINSTRFCPAKDIVIQ 317
           ENLISSKGKLWIFRIRNE NSTRFCP K I+IQ
Sbjct: 241 ENLISSKGKLWIFRIRNEKNSTRFCPDKSILIQ 273

BLAST of Lcy06g004960 vs. NCBI nr
Match: XP_022137311.1 (glucuronoxylan 4-O-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 513.8 bits (1322), Expect = 1.0e-141
Identity = 262/316 (82.91%), Postives = 282/316 (89.24%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           MPP++ADCH LVSSES FSP SV   QQPYPS S KNSRGSK+MNFTR KIIPVL+LVLS
Sbjct: 1   MPPDLADCHPLVSSESLFSPPSVPSLQQPYPSLSLKNSRGSKSMNFTRKKIIPVLVLVLS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TS LL IAL PKLQ P    S N  R SA E AISEKEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSSTSSLLAIALPPKLQRPIFGFSINQSRASANEVAISEKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+AKKAPCNLL+FGLESQYLNLSAINDGG NVFLEDDPYKLSTFKI+SNHTR+YKVDYK 
Sbjct: 121 LIAKKAPCNLLVFGLESQYLNLSAINDGGKNVFLEDDPYKLSTFKISSNHTRIYKVDYKH 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
            AE+AY+LLK AR+NK CAPN+GL N SKCQLALTDLPREVYEVKWDVVVVDGP GD+PQ
Sbjct: 181 PAEKAYKLLKHARQNKACAPNSGLKNLSKCQLALTDLPREVYEVKWDVVVVDGPIGDAPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVV+HDIDR+IEKWYSWEFLCDENLISS+GKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVIHDIDRMIEKWYSWEFLCDENLISSRGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDIVIQ 317
           EINSTRFCP K I+IQ
Sbjct: 301 EINSTRFCPDKGIMIQ 316

BLAST of Lcy06g004960 vs. NCBI nr
Match: KAG6584154.1 (Protein IRREGULAR XYLEM 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019746.1 Protein IRREGULAR XYLEM 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.9 bits (1317), Expect = 3.9e-141
Identity = 260/317 (82.02%), Postives = 280/317 (88.33%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVS-PSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVL 60
           MPPEV D  SLV SES FSP SVS PSQ PYPS +FK SRG K M+ TRNKI+PVL+L+L
Sbjct: 1   MPPEVTDWPSLVPSESFFSPPSVSPPSQHPYPSVNFKISRGIKGMSSTRNKILPVLVLIL 60

Query: 61  SSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLS 120
           SSL I RLVRIALTTSYTSP+L IAL PKLQ P S  S+NH R SA EA ISEKEFKLLS
Sbjct: 61  SSLCIFRLVRIALTTSYTSPILAIALPPKLQRPISGFSRNHSRTSANEATISEKEFKLLS 120

Query: 121 DLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYK 180
           +L+AKK PCNLL FG+ESQYLNLSA+N GG NVFLEDDPYKLS FK+NSNHTRMYKVDYK
Sbjct: 121 NLIAKKVPCNLLFFGIESQYLNLSAVNPGGKNVFLEDDPYKLSIFKVNSNHTRMYKVDYK 180

Query: 181 LHAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSP 240
           LHAE+AY+LLK AR NK+CAPN+GL NSS+CQLALTDLPREVYEVKWDVVVVDGPRGDSP
Sbjct: 181 LHAEKAYKLLKHARGNKSCAPNSGLRNSSRCQLALTDLPREVYEVKWDVVVVDGPRGDSP 240

Query: 241 QAPGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIR 300
            APGRMATIYSAA+IAREGNTTDVVVHDIDR+IEKWYSWEFLCDENLISSKGKLWIFRIR
Sbjct: 241 LAPGRMATIYSAAIIAREGNTTDVVVHDIDRMIEKWYSWEFLCDENLISSKGKLWIFRIR 300

Query: 301 NEINSTRFCPAKDIVIQ 317
           NE NSTRFCP K I+IQ
Sbjct: 301 NEKNSTRFCPDKSILIQ 317

BLAST of Lcy06g004960 vs. NCBI nr
Match: XP_038895890.1 (glucuronoxylan 4-O-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 511.5 bits (1316), Expect = 5.1e-141
Identity = 254/316 (80.38%), Postives = 286/316 (90.51%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           MPPE+ DCHSLV SE S SP S+SPSQQ YPS SF+NSRGSK+M+FTRNKI+PVL+L+LS
Sbjct: 1   MPPELVDCHSLVPSE-SLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTSYTSPLL IAL PKLQ P S  S+NH R SA EAAISE EFKLLS+
Sbjct: 61  SLSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RTSANEAAISETEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+ +KAPCNLL FG+E QYLNLSA+NDGG NVFLEDDPYKLSTF+++SNHTRMYK+DYK 
Sbjct: 121 LIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKF 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
           HAE+AY+LLK AR NK+CAP++GL NSSKC+LALTDLP+EVYEVKWDVVVVDGPRGDSP 
Sbjct: 181 HAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPH 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA++AREGNTTDVVVHD DR+IE+WYSWEFLCDENLISSKGKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDIVIQ 317
           E NS+RFCP K IV+Q
Sbjct: 301 EKNSSRFCPDKRIVVQ 314

BLAST of Lcy06g004960 vs. NCBI nr
Match: KAA0055335.1 (protein IRX15-LIKE [Cucumis melo var. makuwa])

HSP 1 Score: 506.1 bits (1302), Expect = 2.1e-139
Identity = 255/313 (81.47%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           M P V DCHSLV SE + SP S+SPSQQ  PS SF NS+GSKNM+FTRNK++PVL+L+LS
Sbjct: 1   MLPAVEDCHSLVPSE-ALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TSPLL IAL PKLQ P S  S+ H R SA EAAIS KEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+A+K PCNLL FG+ESQYLNLSA+NDGGNNVFLEDDPYKLSTFK+NSNHTRMYKVDYK 
Sbjct: 121 LIAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKF 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
           HAE+AY+LLK AR NK+CAP++GL NSSKC+LALTDLPREVYEVKWDVVVVDGPRGDSPQ
Sbjct: 181 HAEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVVVHD DR+IEKWYSWEFLCDENLISSKGKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDI 314
           E NST FCP K I
Sbjct: 301 EKNSTSFCPDKVI 312

BLAST of Lcy06g004960 vs. NCBI nr
Match: TYJ99260.1 (protein IRX15-LIKE [Cucumis melo var. makuwa])

HSP 1 Score: 506.1 bits (1302), Expect = 2.1e-139
Identity = 255/313 (81.47%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSPLSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVLS 60
           M P V DCHSLV SE + SP S+SPSQQ  PS SF NS+GSKNM+FTRNK++PVL+L+LS
Sbjct: 1   MLPAVEDCHSLVPSE-ALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILS 60

Query: 61  SLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSD 120
           SLSI+RLVRIALTTS TSPLL IAL PKLQ P S  S+ H R SA EAAIS KEFKLLS+
Sbjct: 61  SLSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSN 120

Query: 121 LVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKL 180
           L+A+K PCNLL FG+ESQYLNLSA+NDGGNNVFLEDDPYKLSTFK+NSNHTRMYKVDYK 
Sbjct: 121 LIAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKF 180

Query: 181 HAEEAYQLLKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240
           HAE+AY+LLK AR NK+CAP++GL NSSKC+LALTDLPREVYEVKWDVVVVDGPRGDSPQ
Sbjct: 181 HAEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQ 240

Query: 241 APGRMATIYSAAVIAREGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAA+IAREGNTTDVVVHD DR+IEKWYSWEFLCDENLISSKGKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRN 300

Query: 301 EINSTRFCPAKDI 314
           E NST FCP K I
Sbjct: 301 EKNSTSFCPDKVI 312

BLAST of Lcy06g004960 vs. TAIR 10
Match: AT4G24910.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 248.1 bits (632), Expect = 9.7e-66
Identity = 148/328 (45.12%), Postives = 203/328 (61.89%), Query Frame = 0

Query: 1   MPPEVADCHSLVSSESSFSP-LSVSPSQQPYPSASFKNSRGSKNMNFTRNKIIPVLLLVL 60
           MPP+        SS S  +P L  SP   P  +   +     K   FT  K+IP+L+ VL
Sbjct: 1   MPPQFHQ-----SSLSILNPLLRFSPPSSP-DNPKHQRLSTIKMPKFTVRKLIPLLIFVL 60

Query: 61  SSLSIVRLVRIAL---TTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFK 120
           SSLS++RL+RI+    + S +S      L+P     + +L  N G       A+ EKE K
Sbjct: 61  SSLSVLRLLRISYKSSSKSQSSSSTTFRLSP--ADSSQQLRANEG-----PTALMEKELK 120

Query: 121 LLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFK--INSNHTRMY 180
           LLSD V +++PCN+L+FG   QYL LS+IN  G  V LED+P K+   K  +N N+TR+Y
Sbjct: 121 LLSDTVTRRSPCNILVFGFAPQYLMLSSINTRGITVILEDEPAKIMIPKAEVNPNNTRIY 180

Query: 181 KVDY-KLHAEEAYQLLKLARRNKTCAPNAGLIN--SSKCQLALTDLPREVYEVKWDVVVV 240
            + Y ++    AY LL+ AR N  CAPN    +  SS C+L L DLP++V+  KWDV+VV
Sbjct: 181 SLKYHQMEVRNAYNLLQHARANPACAPNMNNQHQGSSDCKLELRDLPQQVHNTKWDVIVV 240

Query: 241 DGPRGDSPQAPGRMATIYSAAVIAREG---NTTDVVVHDIDRIIEKWYSWEFLCDENLIS 300
           DGPRGD  + PGRM +IY+AAV+AR+G   +TTDV VHD+ R  EKW SWEFLC ENL+S
Sbjct: 241 DGPRGDDLETPGRMGSIYTAAVLARKGSSNSTTDVFVHDVHRTAEKWLSWEFLCQENLVS 300

Query: 301 SKGKLWIFRIRNEINSTRFCPAKDIVIQ 317
           +KG  W FRI+ + N++RFC  + ++I+
Sbjct: 301 AKGTFWKFRIKRQSNASRFCSPETVIIR 315

BLAST of Lcy06g004960 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 152.1 bits (383), Expect = 7.3e-37
Identity = 96/286 (33.57%), Postives = 147/286 (51.40%), Query Frame = 0

Query: 38  SRGSKNMNFTRNKIIPVLLLVLSS-----LSIVRLVRIALTTSYTSPLLGIALAPKLQHP 97
           S+G+K     R   + V L  L        S +R     L+   T+     ++A     P
Sbjct: 6   SKGAKQTLLERPWFLAVALAGLIGGAMLITSFIRATDNTLSLCSTAKNTAASIAKYTATP 65

Query: 98  ASELSKNHGRNSAKEAAISEKEFKLLSDLVAKKAPCNLLIFGLESQYLNLSAINDGGNNV 157
               S  H   S      S +E  +  +++ ++ PCN L+FGL    L  +++N GG  V
Sbjct: 66  IQLQSIVHYATSHTVPQQSFEEISISLNVLKERLPCNFLVFGLGRDSLMWASLNPGGTTV 125

Query: 158 FLEDDPYKLSTFKINSNHTRMYKVDYKLHAEEAYQLLKLARRNKTCAPNAG--LINSSKC 217
           FLE+DP  +     ++   R + V Y+ H  EA +LL   +    C P     +  + KC
Sbjct: 126 FLEEDPEWIEAVLKDAPSLRAHHVQYRTHLSEAGRLLSTYKNEPMCLPAKAFPIRYNEKC 185

Query: 218 QLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMATIYSAAVIARE---GNTTDVVVHD 277
            LALT LP E Y+ +WD+++VD P+G  P+APGRMA I+S+A++AR      TT V +HD
Sbjct: 186 PLALTSLPDEFYDTEWDLIMVDAPKGYFPEAPGRMAAIFSSAIMARNRKGDGTTHVFLHD 245

Query: 278 IDRIIEKWYSWEFLCDENLISSKGKLWIFRIRNEINST-----RFC 309
           ++R +E  ++ EFLC++  ++S G+LW F I N  N T     RFC
Sbjct: 246 VNRKVENAFANEFLCEKYKVNSVGRLWHFEIPNAANMTDQPGDRFC 291

BLAST of Lcy06g004960 vs. TAIR 10
Match: AT5G67210.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 143.3 bits (360), Expect = 3.4e-34
Identity = 97/258 (37.60%), Postives = 142/258 (55.04%), Query Frame = 0

Query: 60  SSLSIVRLVRIALTTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLS 119
           +S ++   V  A+TT+  S L   A+   L + +      H         +S  E K +S
Sbjct: 57  NSATVSSAVNSAVTTATISQLPTTAINAMLHYASRSNDSYH---------MSYGEMKSIS 116

Query: 120 DLVAK-KAPCNLLIFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDY 179
           D++ +   PCNLL+FGL  + L   ++N  G  VF+E++ Y  + F+       ++ V Y
Sbjct: 117 DVLRRCSPPCNLLVFGLTHETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIEVFDVQY 176

Query: 180 KLHAEEAYQL---LKLARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPR 239
              A EA +L   +K A RN+ C P   L+  S C+L L DLP  VY+V WDV++VDGPR
Sbjct: 177 TTKAREARELVSAVKEAARNE-CRPVQNLL-FSDCKLGLNDLPNHVYDVDWDVILVDGPR 236

Query: 240 GDSPQAPGRMATIYSAAVIARE---GN-TTDVVVHDIDRIIEKWYSWEFLCDENLISSKG 299
           GD    PGRM++I++AAV+AR    GN  T V VHD  R +E+    EFLC ENL+ S  
Sbjct: 237 GDGGDVPGRMSSIFTAAVLARSKKGGNPKTHVFVHDYYRDVERLCGDEFLCRENLVESND 296

Query: 300 KLWIFRI-RNEINSTRFC 309
            L  + + + + NST+FC
Sbjct: 297 LLAHYVLEKMDKNSTQFC 303

BLAST of Lcy06g004960 vs. TAIR 10
Match: AT2G15440.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 142.5 bits (358), Expect = 5.8e-34
Identity = 83/231 (35.93%), Postives = 128/231 (55.41%), Query Frame = 0

Query: 73  TTSYTSPLLGIALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSDLVAKKAP-CNLL 132
           +T+ +  L     +  L  P      ++  +S    ++S  E   +S+++    P CNLL
Sbjct: 58  STTSSHSLSSSPSSSSLPPPVLAALLHYTSSSPPNTSMSFLELSTISNIIHSHGPACNLL 117

Query: 133 IFGLESQYLNLSAINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKLHAEEAYQLLKL 192
           IFGL  + L   +IN  G  VF+++ PY +S F+ ++     Y+V Y     +A +LL  
Sbjct: 118 IFGLTHESLLWRSINFQGRTVFVDESPYSVSKFEQSNPGVEAYEVVYSTKVSQAKKLLGY 177

Query: 193 ARRNKTCAPNAGLINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMATIYSA 252
            +    C P   L+  S C+L + DLP  VYE+ WDV+++DGPRG +  +PGRMA I+++
Sbjct: 178 YKTRPECRPVQNLL-FSDCKLGINDLPNFVYEIDWDVILIDGPRGYASDSPGRMAPIFTS 237

Query: 253 AVIAR------EGNTTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIF 297
           AV+A+      +   TDV+VH+  R IE+ YS EFLC+ENLI   G L  F
Sbjct: 238 AVLAKSKDFGTKTKKTDVLVHEFGRKIERVYSEEFLCEENLIEVVGDLGHF 287

BLAST of Lcy06g004960 vs. TAIR 10
Match: AT1G71690.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 139.0 bits (349), Expect = 6.4e-33
Identity = 81/234 (34.62%), Postives = 124/234 (52.99%), Query Frame = 0

Query: 84  ALAPKLQHPASELSKNHGRNSAKEAAISEKEFKLLSDLVAKKAPCNLLIFGLESQYLNLS 143
           +LA  L H AS  S    + +  E ++++KE +       KK+PCN L+FGL    L  +
Sbjct: 71  SLADALVHYAS--SNVTPQQTLSEISVTKKELE-------KKSPCNFLVFGLGHDSLMWA 130

Query: 144 AINDGGNNVFLEDDPYKLSTFKINSNHTRMYKVDYKLHAEEAYQLLKLARRNKTCAPNAG 203
            +N GG  +FL++D   +            Y V YK    +A  L+   +  + C   + 
Sbjct: 131 TLNHGGRTIFLDEDESWIHQIAEKFPSLESYHVRYKTKVRDAEALMAATKDREECRRVST 190

Query: 204 LINSSKCQLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMATIYSAAVIAR----EGN 263
            +  S C+LAL  LP  VYE +WD+++VD P G   +APGRM+ IY+A +IAR    E  
Sbjct: 191 DLRVSTCELALKGLPEVVYETEWDLIMVDAPTGFHEEAPGRMSAIYTAGMIARRRKDEEE 250

Query: 264 TTDVVVHDIDRIIEKWYSWEFLCDENLISSKGKLWIFRIRNE----INSTRFCP 310
           TT V VHD+DR +E  +S EFLC + +   +G+L  F + +     ++  +FCP
Sbjct: 251 TTAVFVHDVDRKVEDEFSMEFLCRDYMTKQEGRLRHFTVPSHRNYGVSGVKFCP 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FH924.8e-3337.60Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1[more]
Q9SNE52.0e-3135.11Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1[more]
Q6NMK12.9e-3033.70Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 P... [more]
Q9T0F72.9e-3037.62Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 P... [more]
Q9C7F94.9e-3037.57Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g2793... [more]
Match NameE-valueIdentityDescription
A0A6J1C6754.9e-14282.91glucuronoxylan 4-O-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5A7UNX61.0e-13981.47Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00... [more]
A0A5D3BHK31.0e-13981.47Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0... [more]
A0A0A0LRK12.8e-13780.77Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G05... [more]
A0A6J1KL986.9e-12884.62glucuronoxylan 4-O-methyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114952... [more]
Match NameE-valueIdentityDescription
XP_022137311.11.0e-14182.91glucuronoxylan 4-O-methyltransferase 1 [Momordica charantia][more]
KAG6584154.13.9e-14182.02Protein IRREGULAR XYLEM 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_038895890.15.1e-14180.38glucuronoxylan 4-O-methyltransferase 1 [Benincasa hispida][more]
KAA0055335.12.1e-13981.47protein IRX15-LIKE [Cucumis melo var. makuwa][more]
TYJ99260.12.1e-13981.47protein IRX15-LIKE [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G24910.19.7e-6645.12Protein of unknown function (DUF579) [more]
AT1G67330.17.3e-3733.57Protein of unknown function (DUF579) [more]
AT5G67210.13.4e-3437.60Protein of unknown function (DUF579) [more]
AT2G15440.15.8e-3435.93Protein of unknown function (DUF579) [more]
AT1G71690.16.4e-3334.62Protein of unknown function (DUF579) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006514IRX15/IRX15L/IGXMTIGRFAMTIGR01627TIGR01627coord: 99..308
e-value: 4.5E-75
score: 250.2
IPR006514IRX15/IRX15L/IGXMPANTHERPTHR31444OS11G0490100 PROTEINcoord: 1..311
IPR021148Polysaccharide biosynthesis domainPFAMPF04669Polysacc_synt_4coord: 114..295
e-value: 3.7E-58
score: 196.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..41
NoneNo IPR availablePANTHERPTHR31444:SF34GLUCURONOXYLAN 4-O-METHYLTRANSFERASE-LIKE PROTEIN (DUF579)coord: 1..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g004960.1Lcy06g004960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0009834 plant-type secondary cell wall biogenesis
biological_process GO:0045492 xylan biosynthetic process
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity