Lcy05g018000 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g018000 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 1.4e-27
Identity = 97/373 (26.01%), Postives = 179/373 (47.99%), Query Frame = 0

Query: 3   PTKAS-GPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSY-PK 62
           PTK S GPDG  + FYQ + + +   +  +  K   E    +S     I+LIPK    P 
Sbjct: 473 PTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGTLPNSFYEATITLIPKPQKDPT 532

Query: 63  SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 122
            + +FRPISL N+  KI+ K LANR++  + +II P +  F+P     +N+      IH 
Sbjct: 533 KIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHY 592

Query: 123 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 182
           + + +  ++ ++ I LD  KA+D+++  F+ KV+   G    +++ +         ++ +
Sbjct: 593 INKLK--DKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKV 652

Query: 183 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 242
           NG          G RQG PLSPYLF +  E L   I ++     + G +I +    ++ L
Sbjct: 653 NGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQK---EIKGIQIGKEEVKISLL 712

Query: 243 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKS-KFMASKN-VSNREVAEISQ 302
             AD   +++   ++       ++  +  V G  IN  KS  F+ +KN  + +E+ E + 
Sbjct: 713 --ADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEKEIRETTP 772

Query: 303 I-LGIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKE---NLFSELNLFNQA 362
             +    ++ LG+ L    +   +K   F  + + +++ +++WK+   +    +N+   A
Sbjct: 773 FSIVTNNIKYLGVTLTKEVKDLYDKN--FKSLKKEIKEDLRRWKDLPCSWIGRINIVKMA 832

Query: 363 LLAKQSWRILRSP 368
           +L K  +R    P
Sbjct: 833 ILPKAIYRFNAIP 836

BLAST of Lcy05g018000 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 8.9e-27
Identity = 93/376 (24.73%), Postives = 177/376 (47.07%), Query Frame = 0

Query: 3   PTKAS-GPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPKS 62
           PTK S GPDG  + FYQ+Y + +   +  +      E    +S     I LIPK     +
Sbjct: 466 PTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTT 525

Query: 63  MAD-FRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 122
             + FRPISL N+  KI+ K LANR+++ +  +I   +  F+P     +N+      I  
Sbjct: 526 KKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQH 585

Query: 123 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 182
           +   R  ++ +V I +D  KA+D+++  F+ K +  +G +  ++  +    +  + ++++
Sbjct: 586 I--NRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIIL 645

Query: 183 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 242
           NG          G RQG PLSP LF +  E L   I +E     + G ++ +    L+  
Sbjct: 646 NGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEK---EIKGIQLGKEEVKLS-- 705

Query: 243 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSK---FMASKNVSNREVAEIS 302
            FAD   ++L+          +++  + +VSG  IN++KS+   +  ++   ++ + E+ 
Sbjct: 706 LFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQIMGELP 765

Query: 303 QILGIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKE---NLFSELNLFNQA 362
             +  K ++ LGI L    +    +   +  +++ +++   KWK    +    +N+   A
Sbjct: 766 FTIASKRIKYLGIQLTRDVKDLFKEN--YKPLLKEIKEDTNKWKNIPCSWVGRINIVKMA 825

Query: 363 LLAKQSWRILRSPDSL 371
           +L K  +R    P  L
Sbjct: 826 ILPKVIYRFNAIPIKL 832

BLAST of Lcy05g018000 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 3.5e-23
Identity = 91/365 (24.93%), Postives = 165/365 (45.21%), Query Frame = 0

Query: 5   KASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTW----ISLIPKTSY-P 64
           K+ GPDG  S FYQ +     +E+  + L           +  T+    I+LIPK    P
Sbjct: 468 KSPGPDGFTSEFYQTF----KEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDP 527

Query: 65  KSMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIH 124
               ++RPISL N+  KI+ K L NR+++ +  II   +  F+P     +N+      I 
Sbjct: 528 TRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQ 587

Query: 125 SLTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVL 184
            + + +  N+ ++ + +D  KA+D ++  F+ + +  +G    ++  +       + +++
Sbjct: 588 HINKLK--NKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANII 647

Query: 185 INGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTH 244
           +NG          G RQG PLSP LF +  E L   I  E +   + G  I      L+ 
Sbjct: 648 LNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIREEKA---IKGIHIGSEEIKLS- 707

Query: 245 LFFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKS-KFMASKNVSNREVAEISQ 304
             FAD   ++L+ +R        +++EY  VSG  IN  KS  F+ + N    +  + S 
Sbjct: 708 -LFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKDSI 767

Query: 305 ILGI--KGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKE---NLFSELNLFNQ 359
              +  K ++ LG+YL    +    +   +  + + + + V KWK    +    +N+   
Sbjct: 768 PFTVVPKKMKYLGVYLTKDVKDLYKEN--YETLRKEIAEDVNKWKNIPCSWLGRINIVKM 819

BLAST of Lcy05g018000 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.5e-21
Identity = 99/366 (27.05%), Postives = 159/366 (43.44%), Query Frame = 0

Query: 1   MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
           M   K+ G DG    F+Q +W  +G +   V  +A  +     S  R  +SL+PK    +
Sbjct: 461 MPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLR 520

Query: 61  SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
            + ++RP+SL +  YKIVAK+++ RLK VL  +I P +   VP R I  NV +  + +H 
Sbjct: 521 LIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHF 580

Query: 121 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
               RR       + LD  KA+DRV+  ++   + A  F   ++  +     SA   V I
Sbjct: 581 ---ARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKI 640

Query: 181 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
           N          RG+RQG PLS  L+ +  E  L L+ +      L G  +      +   
Sbjct: 641 NWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRK-----RLTGLVLKEPDMRVVLS 700

Query: 241 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNV-------SNREV 300
            +AD   I +     +    +   E Y   S   IN  KS  +   ++       + R++
Sbjct: 701 AYAD-DVILVAQDLVDLERAQECQEVYAAASSARINWSKSSGLLEGSLKVDFLPPAFRDI 760

Query: 301 AEISQILGIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENLFSELNLFNQ 360
           +  S+I     ++ LG+YL       S     F  + E V   + KWK      L++  +
Sbjct: 761 SWESKI-----IKYLGVYLSAEEYPVSQN---FIELEECVLTRLGKWK-GFAKVLSMRGR 808

BLAST of Lcy05g018000 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.3e-14
Identity = 89/339 (26.25%), Postives = 147/339 (43.36%), Query Frame = 0

Query: 53  IPKTSYPKSMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVP-DRLITYNV 112
           IPKT   K   DFRPIS+ +V+ + +   LA RL   ++    P +  F+P D       
Sbjct: 403 IPKTVTAKRPQDFRPISVPSVLVRQLNAILATRLNSSIN--WDPRQRGFLPTDGCADNAT 462

Query: 113 IIGFECIHSLTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCV 172
           I+     HS    +     Y+A  LD+SKA+D +    I   + A G   G++D V N  
Sbjct: 463 IVDLVLRHS---HKHFRSCYIA-NLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTY 522

Query: 173 ESASFSVLINGHPYPIEK--PSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFK 232
           E    S  +NG  +  E+  P+RG++QGDPLSP LF +  + LL  +  E+      G K
Sbjct: 523 EGGGTS--LNGDGWSSEEFVPARGVKQGDPLSPILFNLVMDRLLRTLPSEI------GAK 582

Query: 233 INRYCPPLTHLFFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVS 292
           +           FAD   +F +      V+  + L ++  + G  +N +K   +  K   
Sbjct: 583 VGNAI--TNAAAFADDLVLFAETRMGLQVLLDKTL-DFLSIVGLKLNADKCFTVGIKGQP 642

Query: 293 NREVAEI---------SQILGIKGVEE---LGIYLGMPSQGGSNKTRIFHHIVERVRKVV 352
            ++   +         S+I  +K  +E   LGI      +   N        ++R+ K  
Sbjct: 643 KQKCTVLEAQSFYVGSSEIPSLKRTDEWKYLGINFTATGRVRCNPAEDIGPKLQRLTKAP 702

Query: 353 QKWKENLFSEL-----NLFNQALLAKQSWRILRSPDSLV 372
            K ++ LF+        L+++  L   +  +LR  D L+
Sbjct: 703 LKPQQRLFALRTVLIPQLYHKLALGSVAIGVLRKTDKLI 724

BLAST of Lcy05g018000 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 7.3e-141
Identity = 258/576 (44.79%), Postives = 349/576 (60.59%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            M PTKA GPDG  +LFYQ YW  VG +    CL ALN   DI   N T+I+LIPK   P+
Sbjct: 602  MFPTKALGPDGFPALFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPR 661

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
            S++DFRPISLCNV YKI++KS+ NRLK V+  +IS  + AFVP R I+ NVIIG EC+H+
Sbjct: 662  SISDFRPISLCNVSYKIISKSITNRLKNVIGLVISDAQSAFVPSRAISDNVIIGHECLHT 721

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            +   + G  G  A+KLD+SKA+DRVEW +++ +M  MGFN+GWI  ++ C+ +  FS+ +
Sbjct: 722  INSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHL 781

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG P    +PSRG+RQGDPLSPYLFL+CAEGL ALIN E +   L G         +THL
Sbjct: 782  NGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSALINHENNSGRLTGIHFEENNTSITHL 841

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
             FAD S IFL++  SEC+  +R+L+ Y R SGQ IN  KS  + S NV       +  IL
Sbjct: 842  LFADDSLIFLRSLESECLALRRLLDSYGRASGQCINFSKSALLFSPNVHPERQQYLQCIL 901

Query: 301  GIKGVEELGIYLGMPS-----QGGSNKTRIFHHIVERVRKVVQKWKENL-FSELNLFNQA 360
             +K V   G YLG+PS     +G S K     H ++  R    K    L F +L  FNQA
Sbjct: 902  NVKLVSHFGNYLGLPSHFTRRRGESRKL----HWMKWGRMCYPKECGGLNFRDLEGFNQA 961

Query: 361  LLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFVQGFRW 420
            L+AK  WR L+ P+ LV++VLK KYF++   L+A   SK+S+ W+  +WGRDL V+G R 
Sbjct: 962  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1021

Query: 421  KVGSGKCISIDQDPWIDRRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEIFCPADA 480
            +VG+G  I    DPW+ R   F P+  N+        S    DGNW+   I   FC  D 
Sbjct: 1022 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNWDVTSISHSFCNEDR 1081

Query: 481  EAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENPSWKKL 540
            + IL+MP  S +L D  +W YD +G +SV+S Y+L + +     ++++N+      W  +
Sbjct: 1082 DLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSASTNY--RGTQWNSI 1141

Query: 541  WKSDIMPKAKIEVWRIINNAIPTQKNICARGIGVNP 571
            WK  +  K KI +WR  +  IPT +N+  RGIG  P
Sbjct: 1142 WKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELP 1171

BLAST of Lcy05g018000 vs. ExPASy TrEMBL
Match: A0A803PI64 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 7.1e-136
Identity = 288/758 (37.99%), Postives = 412/758 (54.35%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            MNPTKA G DG  +LFY K+W  +  +V  +CLK LN  A++  +N T I+LIPK   PK
Sbjct: 692  MNPTKAPGCDGLPALFYHKFWSNLKQDVIGMCLKVLNHGANLECLNETIIALIPKVEKPK 751

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
             + +FRPISLCNVIYKIV+K L  RL  V+D +IS T+ AF+ DRLI  N IIG+E +H 
Sbjct: 752  KVEEFRPISLCNVIYKIVSKCLVARLSGVMDLVISDTQSAFIKDRLIHDNAIIGYESLHC 811

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            + + R  N G +A+KLDM+KAYDRVEW+F+  VM  +GF   W+DK+M CV S SFS LI
Sbjct: 812  MRKNRFQNGGKMALKLDMAKAYDRVEWLFLSAVMSRLGFAQCWVDKIMRCVTSTSFSFLI 871

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG       P RGLRQGDPLSP+LFL CAE L +LI +E S   L G + NR    ++HL
Sbjct: 872  NGETKGKLIPERGLRQGDPLSPFLFLFCAEALSSLIQQEESAGRLRGIRFNRLGVSVSHL 931

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
            FFAD S +F+ A    C+ F++IL +Y   SGQ +N  KS+     NVS     +++ ++
Sbjct: 932  FFADDSLVFIDADMDSCLQFQQILTKYTAASGQIVNYHKSEACFGCNVSAETRFQLAGMM 991

Query: 301  GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQ-KW--------KENL-FSELNL 360
            G++ V+  G YLG+PS  G NK      I  + +K+   KW        K  L F +L +
Sbjct: 992  GVREVDNHGKYLGLPSFVGRNKKEFLDEIKNKEKKIHWCKWRYLCRPKDKGGLGFRDLGM 1051

Query: 361  FNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFVQ 420
            FNQALLAKQ WR +R P  L +RVLK+ YF    FL A   + ASF WRS+VWG+ L ++
Sbjct: 1052 FNQALLAKQIWRCIRHPQQLCSRVLKASYFPHKGFLEAGCGANASFVWRSLVWGKKLILK 1111

Query: 421  GFRWKVGSGKCISIDQDPWIDRRGIFSPIWVNDSLRGRLGCS-IRKGDGNWNEELIKEIF 480
            G+RW+VG+G+ + + +DPW+ R   F  ++   +L   L  + +++ DG W+E  I+ +F
Sbjct: 1112 GYRWRVGNGESVRVLEDPWLPRPLTFK-VYDQPTLPENLYVTDLKRADGQWDESFIRSVF 1171

Query: 481  CPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENP 540
               DAE IL +P    D  D+I+  Y   G ++VKS YR+A  + +    S+ + L +  
Sbjct: 1172 NTIDAELILAIPFSDCDFEDKILLHYSKNGEYTVKSGYRMASSLITEHHQSSDHSLVQ-- 1231

Query: 541  SWKKLWKSDIMPKA------------------------------------KIEVWRIINN 600
             WKKLW+  I PKA                                    K+E + +++ 
Sbjct: 1232 WWKKLWRLKIPPKASKGYWVVSGVYDEFKKMLGTDNLSLLMRMAAEWEKEKLEFFLLVSW 1291

Query: 601  AIPTQKNICARGIGVNPMAPKPSVESC-------KGHAS------------WSPPPPSSW 660
             +   +N    G   +P  P+  +E C       +G A             W P      
Sbjct: 1292 NVWNVRNSVVHG-SYHP-KPEDMIEWCGRYLDEFRGEAGSRSNSAMVEERRWVPSVVDQV 1351

Query: 661  KLNSDASKNSSSSGGSIGWVLRDSFGSLICAGARLISWNWDVKTLEAYAILEGLKS-IVR 692
             +N DA          +G V+RDS G+++CA A ++        L   AI +GL++ + R
Sbjct: 1352 TINVDAGVKPGGLHSGLGNVMRDSAGAVLCAAATVLQQELPPLQLVLMAIKKGLQAGLQR 1411

BLAST of Lcy05g018000 vs. ExPASy TrEMBL
Match: A0A803Q9W0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 4.3e-133
Identity = 252/642 (39.25%), Postives = 359/642 (55.92%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            +N  KA G DG   LFY+K+W+ +G ++  VCL  LN N D   +N+T + LIPK   PK
Sbjct: 885  INTMKAPGIDGMPRLFYEKHWEVIGRDITKVCLDILNNNKDCRQLNKTLLCLIPKIRQPK 944

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
             + D+RPISLCNV YKI+AK LANR+K  L  +IS  + AF+  RLI  N I+GFE +H 
Sbjct: 945  QVGDYRPISLCNVSYKIIAKCLANRMKDSLKEVISENQSAFIRGRLIQDNAILGFESLHC 1004

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            + + R GN   +A+KLDMSKAYDRVEW F++ +M  +G++  W+DK+MNC++S SFS+L+
Sbjct: 1005 MKKGRFGNGRKMALKLDMSKAYDRVEWRFLETMMICLGYDKRWVDKIMNCIKSISFSILL 1064

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG       PSRGLRQGDPLSPY+FL+C+EGL  LI        L+G +  R    L+HL
Sbjct: 1065 NGDVSGQIHPSRGLRQGDPLSPYMFLLCSEGLSCLIQEAERANRLHGLRFGRDNIKLSHL 1124

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
            FFAD SFIFL A+ S+C   K IL+EY  +SGQ IN +KS+    K +++ +   ++ IL
Sbjct: 1125 FFADDSFIFLDATPSDCQSLKSILDEYSLLSGQRINFDKSELCVGKQINHGDGILLAAIL 1184

Query: 301  GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENLFSE------------- 360
            G+K V+    YLG+P+  G  K  +F  I  ++R  +Q WK +LFS+             
Sbjct: 1185 GVKLVDCHTKYLGIPASVGKKKKEVFEDIRTKIRTKLQGWKASLFSQAGREILLKAIIQA 1244

Query: 361  ----------------------------------------------------------LN 420
                                                                      L 
Sbjct: 1245 IPTYIMSCFRLPKELIKDIHAMMARFWWGSSDTKQKTHWGNWKKLCKPKEKGGMGFKNLE 1304

Query: 421  LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
            LFNQ+LLAKQ W+I+ +P S++ARVLK+ Y+   +FL A      S+ WRSI+WGR +  
Sbjct: 1305 LFNQSLLAKQGWKIINNPHSMLARVLKACYYTNSNFLEAKVGGFGSYMWRSILWGRQIID 1364

Query: 481  QGFRWKVGSGKCISIDQDPWIDRRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEIF 540
            +G RW+V +G+ + I++D W+ R   FS        +G    +I+  DG+WN + I+E F
Sbjct: 1365 KGIRWRVMAGRDVRINEDKWLPRPSTFSLRTPAHVPQGTTINTIKDEDGHWNTQKIEECF 1424

Query: 541  CPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENP 572
             P D   IL +  GST   D++IW Y   G ++V S Y++      +  A TSN      
Sbjct: 1425 HPDDVPLILGITSGSTTQNDDLIWHYTPDGCYTVSSGYKVGTNNEQI--AGTSNDNDAKK 1484

BLAST of Lcy05g018000 vs. ExPASy TrEMBL
Match: A0A7N2L6Z9 (Reverse transcriptase domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 3.7e-132
Identity = 280/662 (42.30%), Postives = 361/662 (54.53%), Query Frame = 0

Query: 1   MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
           ++PTK+ GPDG  ++F+QKYW  VG  V  + L  LN    +  IN+T I LIPKTS PK
Sbjct: 319 IHPTKSPGPDGMSAIFFQKYWDIVGSNVSNMILNVLNYGMSLDVINKTNIVLIPKTSNPK 378

Query: 61  SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
            M DFRPISLCNVIYK+++K+LANRLK  L  II+  + AF  DRLIT NV+I +E +H 
Sbjct: 379 RMTDFRPISLCNVIYKLISKTLANRLKAFLPLIITENQSAFTADRLITDNVLIAYELMHY 438

Query: 121 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
           L  ++ G + ++A KLDMSKA+DRVEW FI++VM  MGFN+GWI  +M C+ S S+SV+I
Sbjct: 439 LKHKKDGKDCFMATKLDMSKAFDRVEWGFIERVMRKMGFNEGWISLIMRCISSVSYSVII 498

Query: 181 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
           NG  +    P+RGLRQGDPLSPYLFL+CAEGL AL++       LNG  + R CP +THL
Sbjct: 499 NGEYFGNIVPTRGLRQGDPLSPYLFLLCAEGLSALLHDAARNQLLNGISLCRGCPRITHL 558

Query: 241 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
           FFAD S +F KA+R EC   K ILE+YE  SGQ +N +KS    S N +      I  IL
Sbjct: 559 FFADDSLLFCKANREECEKLKEILEKYEAASGQKVNSDKSSIFFSPNTTPELKETIFNIL 618

Query: 301 GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENL---------------- 360
           G         YLG+PS  G +K  +F  I ERV   +  WK  L                
Sbjct: 619 GPMQDSRHNKYLGLPSIIGRSKKLVFAEIKERVGLKLAGWKGKLLSSGGKEILIKAVAQA 678

Query: 361 -------------------------------------------------------FSELN 420
                                                                  F  L+
Sbjct: 679 IPTYTMSCFLLPKSLCDELEKMMRNFWWGQKNQESKVAWISWRKMCKPKSLGGLGFRNLH 738

Query: 421 LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
            FN ALLAKQ+WRIL +P SL AR+LK+KYF  GD L A   S  S+TWRSI    ++  
Sbjct: 739 AFNLALLAKQAWRILTNPGSLAARILKAKYFPYGDVLNASLGSNPSYTWRSIYNSLEVLK 798

Query: 481 QGFRWKVGSGKCISIDQDPWIDRRG---IFSPIWVNDSLRGRLGCSIRKGDGNWNEELIK 540
           +G RW+VG+G+ I I  D W+       + +P  + +     +   I      W  + I+
Sbjct: 799 KGTRWRVGNGRRIHIWDDKWLPSPSTYKVITPPRITEDY-PMVSSLIDPDTRWWKIDSIR 858

Query: 541 EIFCPADAEAILNMPGGSTDLPDE-IIWGYDSKGRFSVKSAYRLAV---EVASLKEASTS 584
            +F P DAEAIL +P  S +LPD+ IIW  + KG FSVKSAY +AV   E A + E S+ 
Sbjct: 859 ALFLPVDAEAILKIP-LSFNLPDDRIIWIGNKKGEFSVKSAYFVAVNLLESAEIVECSSG 918

BLAST of Lcy05g018000 vs. ExPASy TrEMBL
Match: A0A803P996 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 4.8e-132
Identity = 288/836 (34.45%), Postives = 424/836 (50.72%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            M P K+ G DG  ++FYQK W  VGD V  V L  LN+NAD +++N+T I+LIPK   P+
Sbjct: 856  MGPDKSPGIDGMSAMFYQKNWTTVGDLVTKVVLSILNDNADPTTLNKTLITLIPKVKKPQ 915

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
             + ++RPISLCNVI K+V K L +R K  L  +IS  + AF+P+RLIT NV++ FE +H+
Sbjct: 916  HIQEYRPISLCNVISKLVTKVLVSRFKIALPMVISEEQSAFLPNRLITDNVLVAFELVHA 975

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            +  +  G  G  + KLDMSKA+DRVEW FI++VM  MGF + WI  +M+C+ + +FS +I
Sbjct: 976  IKNKTAGRNGVASFKLDMSKAFDRVEWKFIEEVMRKMGFAERWISLIMSCLTTNNFSFII 1035

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG       PSRGL+QG PLSPYLFL+C+EG   L+  E    NL GFK+ R+ PP+THL
Sbjct: 1036 NGEVMGDLTPSRGLQQGCPLSPYLFLICSEGFSRLLQHEQDSHNLMGFKLTRHAPPITHL 1095

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
            FFAD S +F +A+   C+  KR+L+ Y + SGQ +NL+KS    S N +        Q L
Sbjct: 1096 FFADDSLLFCQANERSCLAIKRVLDTYHKASGQELNLDKSVMSFSPNTTLAAQVFFHQQL 1155

Query: 301  GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENLFS-------------- 360
             +   E    YLG+PS  G +K  +F +I ER+RK++  W E +FS              
Sbjct: 1156 SMPICECHEKYLGLPSYSGRDKKEMFSNIKERIRKLMNSWNEKIFSAAYFCKQVESMMAN 1215

Query: 361  ---------------ELNL-----------------FNQALLAKQSWRILRSPDSLVARV 420
                             NL                 FNQALLAKQ+WRI   P SL+ R+
Sbjct: 1216 FWWGLTENGSRIHWRSWNLLCKSKLEGGMGFRSFIHFNQALLAKQAWRIFEKPHSLLGRI 1275

Query: 421  LKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFVQGFRWKVGSGKCISIDQDPWIDRRG 480
            LKS+YF   +FL A      S TW+ I W R+L ++G RWKVG G+ I    DPWI    
Sbjct: 1276 LKSRYFPNNNFLEASLGHSPSLTWQGIHWARELLIKGLRWKVGDGRHIQSGSDPWIPGHN 1335

Query: 481  IFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEIFCPADAEAILNMPGGSTDLPDEIIWG 540
             F P   +    G +  ++   +  W+   +++ F   D E IL++P       D +IW 
Sbjct: 1336 TFLPFHYSGPSNGVVS-NLITDERQWDLPSLQQFFSALDVERILSVPLSFFSSRDVLIWH 1395

Query: 541  YDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENPSWKKLWKSDIMPKAKIEVWRIINNA 600
            + S G ++VKS Y LA ++  + ++S+SN   ++  WK  W   + PK KI  WR I++A
Sbjct: 1396 HHSSGLYTVKSGYHLAADIDIIDQSSSSNR--DSHWWKFFWSLQLPPKVKIFAWRAIHDA 1455

Query: 601  IPTQKNICARGIGVNPMAP--KPSVESCKGHASWSPPPPSS----------WKLN----- 660
            +P   ++  R +  +      K + ES  GHA +S     +          W+L+     
Sbjct: 1456 LPVATSLVKRKVITDSTCSVCKQAWESV-GHALFSCRYAKAVWHHSNLVFDWRLSAAMYK 1515

Query: 661  --------------------------------------SDASKNSSSSGGS--------- 720
                                                  + ++K+ +S   +         
Sbjct: 1516 GDYILHLSTIYSKAEMEQIFCSLWTIWTERNRIVHGQKARSAKDLASYAVTYLLNYRNAQ 1575

BLAST of Lcy05g018000 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-140
Identity = 258/576 (44.79%), Postives = 349/576 (60.59%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            M PTKA GPDG  +LFYQ YW  VG +    CL ALN   DI   N T+I+LIPK   P+
Sbjct: 602  MFPTKALGPDGFPALFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPR 661

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
            S++DFRPISLCNV YKI++KS+ NRLK V+  +IS  + AFVP R I+ NVIIG EC+H+
Sbjct: 662  SISDFRPISLCNVSYKIISKSITNRLKNVIGLVISDAQSAFVPSRAISDNVIIGHECLHT 721

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            +   + G  G  A+KLD+SKA+DRVEW +++ +M  MGFN+GWI  ++ C+ +  FS+ +
Sbjct: 722  INSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHL 781

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG P    +PSRG+RQGDPLSPYLFL+CAEGL ALIN E +   L G         +THL
Sbjct: 782  NGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSALINHENNSGRLTGIHFEENNTSITHL 841

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
             FAD S IFL++  SEC+  +R+L+ Y R SGQ IN  KS  + S NV       +  IL
Sbjct: 842  LFADDSLIFLRSLESECLALRRLLDSYGRASGQCINFSKSALLFSPNVHPERQQYLQCIL 901

Query: 301  GIKGVEELGIYLGMPS-----QGGSNKTRIFHHIVERVRKVVQKWKENL-FSELNLFNQA 360
             +K V   G YLG+PS     +G S K     H ++  R    K    L F +L  FNQA
Sbjct: 902  NVKLVSHFGNYLGLPSHFTRRRGESRKL----HWMKWGRMCYPKECGGLNFRDLEGFNQA 961

Query: 361  LLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFVQGFRW 420
            L+AK  WR L+ P+ LV++VLK KYF++   L+A   SK+S+ W+  +WGRDL V+G R 
Sbjct: 962  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1021

Query: 421  KVGSGKCISIDQDPWIDRRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEIFCPADA 480
            +VG+G  I    DPW+ R   F P+  N+        S    DGNW+   I   FC  D 
Sbjct: 1022 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNWDVTSISHSFCNEDR 1081

Query: 481  EAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENPSWKKL 540
            + IL+MP  S +L D  +W YD +G +SV+S Y+L + +     ++++N+      W  +
Sbjct: 1082 DLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSASTNY--RGTQWNSI 1141

Query: 541  WKSDIMPKAKIEVWRIINNAIPTQKNICARGIGVNP 571
            WK  +  K KI +WR  +  IPT +N+  RGIG  P
Sbjct: 1142 WKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELP 1171

BLAST of Lcy05g018000 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 485.7 bits (1249), Expect = 6.8e-133
Identity = 256/653 (39.20%), Postives = 370/653 (56.66%), Query Frame = 0

Query: 1   MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
           M+PTKA GPDG  ++F+QKYW  VG+++  + L  LN N  +  IN+T I+L+PK   P 
Sbjct: 280 MHPTKAPGPDGMSAIFFQKYWNIVGNDIVCMVLDVLNSNMSMVEINKTNITLVPKIKNPT 339

Query: 61  SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
            M+DFRPISLCNV+YK+++K LANRLK +L  IIS  + AF+  RLIT NV++ FE +H 
Sbjct: 340 KMSDFRPISLCNVVYKLISKVLANRLKNILPQIISENQSAFLSGRLITDNVLVAFELMHY 399

Query: 121 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
           L  ++ G EG+ AIKLDMSKAYDRVEW FIK+VM  MGF++ WI  VM+C+ S S+S+L+
Sbjct: 400 LEHKKEGKEGFAAIKLDMSKAYDRVEWGFIKQVMEKMGFHEKWIKLVMHCITSVSYSILV 459

Query: 181 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
           NG  Y    P+RGLRQGDP+SPY+FL+CA+G  +L+N       ++G  I R CP +THL
Sbjct: 460 NGGAYGSITPTRGLRQGDPISPYIFLLCADGFSSLLNDVARKLRISGVSICRGCPKITHL 519

Query: 241 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
           FFAD S +F KA+  EC     IL+ YE  SGQ IN++KS    S N  + +  E+ ++L
Sbjct: 520 FFADDSLLFCKANSQECQTLIDILQLYEDASGQKINVDKSSVFFSNNTPDEKRCEVLRML 579

Query: 301 GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENL---------------- 360
           G         YLG+PS  G +K  IF  + ERV + +  WKE L                
Sbjct: 580 GHMQDTRHKKYLGLPSIIGKSKVEIFAEVKERVERKLSGWKEKLLSVGGREILIKAVAQA 639

Query: 361 -------------------------------------------------------FSELN 420
                                                                  F  L 
Sbjct: 640 IPTYTMSCFQIPKTLCEEIEAMMRRFWWGQRGQESKIAWVSWKKLCKAKKNGGMGFRNLQ 699

Query: 421 LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
            FN A+LAKQ WR++ +P+SLVA++ K++Y+  GD  +A   +  S+TWRSI  G ++  
Sbjct: 700 AFNLAMLAKQGWRLISNPNSLVAQIYKARYYPHGDVFQAKLGASPSYTWRSIFNGLEVVR 759

Query: 481 QGFRWKVGSGKCISIDQDPWID---RRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIK 540
           +G RW+VG+G+ I I +D W+       + SP    D    R+   I +    W +++++
Sbjct: 760 RGTRWRVGNGERILIWEDKWLPTPITYKVISPPKPFDDY-PRVSALIDRERKRWKDDVVR 819

Query: 541 EIFCPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVA-SLKEASTSNHL 579
           ++F P +A  IL++P       D+IIW  + KG FSVKSAY +AV V  +L+   +S+  
Sbjct: 820 DLFLPFEARTILSIPLSHNFPEDQIIWVGNRKGEFSVKSAYYIAVGVIDNLEVGESSSGD 879

BLAST of Lcy05g018000 vs. NCBI nr
Match: XP_042974784.1 (uncharacterized protein LOC122306423 [Carya illinoinensis])

HSP 1 Score: 483.8 bits (1244), Expect = 2.6e-132
Identity = 296/837 (35.36%), Postives = 430/837 (51.37%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            M+P+KA GP+G   LF+QKYW  +G+ +    L ALN       +N T+I+LIPK   P 
Sbjct: 289  MHPSKAPGPNGMSPLFFQKYWGVIGNSITTALLSALNSGMFPKGLNHTFITLIPKKVSPS 348

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
             +ADFRPISLCNV+YKI++K +ANRLKR+L  +IS ++ AFVP R I+ NV+I +E +H 
Sbjct: 349  KVADFRPISLCNVLYKILSKVIANRLKRILPDVISDSQSAFVPGRQISDNVLIAYELLHF 408

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            L  +R+G +G++++KLDMSKAYDRVEW+F++K+M ++GF+   I  VM CV + SFSVL+
Sbjct: 409  LRTKRKGRKGFMSLKLDMSKAYDRVEWMFLEKIMESLGFDKKLISLVMLCVRTVSFSVLV 468

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG+P     PSRGLRQGDPLSPYLFL+C EGL++L+ R V    ++G +I R  P + HL
Sbjct: 469  NGNPKGPIIPSRGLRQGDPLSPYLFLLCTEGLISLLKRNVGREGVDGIRICRGAPRINHL 528

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
             FAD S  F KA  S  +  + +L +YER SGQ IN EK+  + SKNV +    +I Q+ 
Sbjct: 529  LFADDSVFFCKADVSTNMKIQCLLSKYERASGQCINKEKTSMVFSKNVKDDLKRDIMQLW 588

Query: 301  GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENL---------------- 360
            G    ++   YLG+P   G +K + F  I +RV + +Q WK NL                
Sbjct: 589  GGSFTQQYEKYLGLPPMVGRSKKQAFSDIKKRVWQKLQAWKGNLLSQGGREVLIKAVAMS 648

Query: 361  -------------------------------------------------------FSELN 420
                                                                   F +L+
Sbjct: 649  IPTYAMSCFLFPKTLCHELEMMMAKFWWGDQSQENKIHWCSWEKLCVSKFQGRMGFRDLH 708

Query: 421  LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
            LFN ALLAKQ WR+LR+ DSL+ +V K+KYF       A A + +S+ W+ I+   D   
Sbjct: 709  LFNLALLAKQGWRLLRNEDSLLYKVYKAKYFPSSSLFEAKAGANSSYVWKGILEALDCLR 768

Query: 481  QGFRWKVGSGKCISIDQDPWI-DRRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEI 540
            +G RW+VG+G+ I I +DPW+ +   + + I V+++L  ++   I    G WN  +++ +
Sbjct: 769  KGCRWRVGNGQTIRIFKDPWLPEGVSVSALIEVDENL--KVNSLIDANTGWWNVHMMRAL 828

Query: 541  FCPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAEN 600
            F P   + IL +   S +  D + W ++  G FSVKSAYR   +   L    +S+  +E 
Sbjct: 829  FNPNLIQQILKL-HISVNSEDSLYWSHEKNGSFSVKSAYRFIQQHHHLLYGQSSSGSSEA 888

Query: 601  PSWKKLWKSDIMPKAKIEVWRIINNAIPTQKN-------------ICARG---------- 660
              WK LW   +  K KI  WR     +PT  N             +C +G          
Sbjct: 889  VFWKSLWHLKLPKKMKIFAWRACQEKLPTYLNLKKRHVLDDATCVLCNQGMEDAAHALRN 948

Query: 661  ------------IGVNPM--------------APKPSVESCKGHASWSPPPPSSWKLNSD 716
                        + VN                A  P +        W PPP    KLN D
Sbjct: 949  KQLYENISMPFNVTVNNALALEKEYEQVHLFDASNPKISKV---VRWHPPPSDFLKLNID 1008

BLAST of Lcy05g018000 vs. NCBI nr
Match: VVA38592.1 (PREDICTED: reverse mRNAase, partial [Prunus dulcis])

HSP 1 Score: 480.7 bits (1236), Expect = 2.2e-131
Identity = 257/648 (39.66%), Postives = 361/648 (55.71%), Query Frame = 0

Query: 1   MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
           M P+KA GPDG   LFYQKYW+ VGD+V A     L  N  +  +N T+++LIPK   P+
Sbjct: 56  MQPSKAPGPDGLPPLFYQKYWRIVGDDVVAAIRAFLQSNDMLRQLNHTFVTLIPKVKEPR 115

Query: 61  SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
           +MA   PISLCNV+Y+I AK+LANR+K V+ S+IS ++ AFVP RLIT N I+ FE  H 
Sbjct: 116 TMAQLHPISLCNVLYRIGAKTLANRMKFVMQSVISESQSAFVPGRLITDNSIVAFEIAHF 175

Query: 121 LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
           L QRRRG +G +A+KLDMSKAYDRVEW F++K+M AMGF   W+  VM+CV + S+S L+
Sbjct: 176 LKQRRRGRKGSLALKLDMSKAYDRVEWEFLEKMMLAMGFPIPWVRMVMDCVTTVSYSFLV 235

Query: 181 NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
           NG P  I  P+RGLRQGDPLSPYLFL+CAEG   L+++      L G  I R  P ++HL
Sbjct: 236 NGEPTRILYPTRGLRQGDPLSPYLFLLCAEGFTTLLSKAERQGQLQGIVICRGAPTVSHL 295

Query: 241 FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
           FFAD SF+F KA+ + C V K I E YER SGQ IN +KS    S N+     + ++ +L
Sbjct: 296 FFADDSFVFAKATDNNCGVLKHIFEVYERASGQQINCQKSCVAFSTNIHMDTQSRLASVL 355

Query: 301 GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENL---------------- 360
           G+  V+    YLG+P   G NKT  F ++ ERV K +Q W+E                  
Sbjct: 356 GVPRVDSHATYLGLPMMLGRNKTFCFRYLKERVWKKLQGWREQTLSIAGKEVLLKVVAQS 415

Query: 361 -------------------------------------------------------FSELN 420
                                                                  F  L 
Sbjct: 416 IPLYVMSCFLLPQGLCHEIEQMMARFWWGQQGENRKIHWMRWERLCKAKTEGGMGFRCLQ 475

Query: 421 LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
            FN A+LAKQ WR++ +P SL +R+LK+KYF + +F  A   S+ S  W+SI   R +  
Sbjct: 476 AFNMAMLAKQGWRLVHNPHSLASRLLKAKYFPQTNFWEATLGSRPSCVWKSIWTARKVLE 535

Query: 481 QGFRWKVGSGKCISIDQDPWIDRRGIFSPI-----WVNDSLRGRLGCSIRKGDGNWNEEL 540
            G R+++G GK + I  D W+ R   F+ I      + ++    L C+  +G   W+ + 
Sbjct: 536 MGSRFQIGDGKSVRIWGDKWVPRPATFAVITSPLDGMENTKVSELICN--EGSPQWDLQK 595

Query: 541 IKEIFCPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKE-ASTSN 572
           +  +F P D   I+ +P      PD I+W YD  G F+VKSAYR+A+ V S  E  S+S+
Sbjct: 596 LNNLFLPVDVVDIVRIPLSIRAPPDRIVWNYDKHGLFTVKSAYRVALRVTSGDEDESSSS 655

BLAST of Lcy05g018000 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-129
Identity = 290/898 (32.29%), Postives = 420/898 (46.77%), Query Frame = 0

Query: 1    MNPTKASGPDGAHSLFYQKYWKGVGDEVCAVCLKALNENADISSINRTWISLIPKTSYPK 60
            M PTKA GPDG  + F+QK+W+ VG+ +   CL  LNE   + S+N T+I+LIPK   P+
Sbjct: 535  MCPTKAPGPDGLPAAFFQKHWQIVGEGLTKTCLHILNEQGTLDSLNHTFIALIPKVEKPR 594

Query: 61   SMADFRPISLCNVIYKIVAKSLANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHS 120
             + +FRPISLCNV+Y+IVAK++ANRLK +L+ IISP + AF+P+RLIT NVIIG+EC+H 
Sbjct: 595  KVMEFRPISLCNVVYRIVAKAIANRLKPILNHIISPNQSAFIPNRLITDNVIIGYECLHK 654

Query: 121  LTQRRRGNEGYVAIKLDMSKAYDRVEWVFIKKVMGAMGFNDGWIDKVMNCVESASFSVLI 180
            +   +    G VA+KLD+SKAYDRVEW F+++ M  +GF+  WI  +M+C+ +  FSVLI
Sbjct: 655  IRLSKGRRNGLVALKLDISKAYDRVEWNFLEQTMSNLGFSAKWISLIMSCITTTCFSVLI 714

Query: 181  NGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLTHL 240
            NG+P  + KP RGLRQG PLSPYLF++CAE    L+N+      + G K  +    +THL
Sbjct: 715  NGNPVGLIKPERGLRQGCPLSPYLFILCAEAFSNLLNQAEREQKIRGLKFAQDI-TITHL 774

Query: 241  FFADGSFIFLKASRSECVVFKRILEEYERVSGQAINLEKSKFMASKNVSNREVAEISQIL 300
             FAD S +F KAS ++C   K I + Y + SGQ  N EKS    S   S+ +++ I  I 
Sbjct: 775  LFADDSLVFSKASVADCKYLKGIFDCYAKASGQIFNFEKSSMFFSGKASSEQISAIKSIF 834

Query: 301  GIKGVEELGIYLGMPSQGGSNKTRIFHHIVERVRKVVQKWKENLFS-------------- 360
             +K V +   YLG+P   G NK   F  +  +V   +  W   LFS              
Sbjct: 835  QLKVVPKYEKYLGLPPMLGRNKMSFFKEVKLKVTSKISSWHHKLFSAGGKEILIKAVAQA 894

Query: 361  ---------------------------------------------------------ELN 420
                                                                     +L 
Sbjct: 895  VPAYAMSVFKLPKGLCEDIQKEIARFWWGTKKDKHGIHWARWDSMSKAKRRGGLGFRDLP 954

Query: 421  LFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFV 480
             FNQAL+AKQ WR++R P+SL+ARV+K++Y++   F  A   S  SF WRSI+WG  +  
Sbjct: 955  SFNQALVAKQGWRLVRYPNSLMARVMKARYYKNSTFWNAKVGSNPSFIWRSILWGSQVIK 1014

Query: 481  QGFRWKVGSGKCISIDQDPWIDRRGIFSPIWVNDSLRGRLGCSIRKGDGNWNEELIKEIF 540
            +G RW++G GK + + +D WI R   F PI         +   +   +  W  + +++ F
Sbjct: 1015 KGVRWRIGDGKKVLVYKDKWIPRPATFQPISPKTLPHETVVADLIDSENKWRVDRLEQHF 1074

Query: 541  CPADAEAILN--MPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAE 600
               D EAIL   +P G  +  DE++W +D KG +SVKS Y+LA+      E  +SN  + 
Sbjct: 1075 MKEDIEAILKILLPSGKEE--DEVLWHFDKKGEYSVKSGYQLALNQNFPNEPESSN--SS 1134

Query: 601  NPSWKKLWKSDIMPKAKIEVWRIINNAIPTQKNICARGIGVNPMAPKPSVE--------- 660
            +  WK  W  D+  K KI +WR + N +PT +N+  R     P+  +  ++         
Sbjct: 1135 SRLWKIPWMLDLPEKVKIFMWRALKNILPTAENLWKRRSLQEPICQRCKLQVETVSHVLI 1194

Query: 661  SCKG-------------------------------------------------------- 720
             CK                                                         
Sbjct: 1195 ECKAARKIWDLAPLIVQPSKDHNQDFFSAIQEMWSRSSTAEAELMIVYCWVIWSARNKFI 1254

BLAST of Lcy05g018000 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 1.4e-30
Identity = 69/224 (30.80%), Postives = 123/224 (54.91%), Query Frame = 0

Query: 345 FSELNLFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWG 404
           F ++  FN ALL KQ WR+L  P+SL+A+V KS+YF + D L AP  S+ SF W+SI   
Sbjct: 58  FKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHAS 117

Query: 405 RDLFVQGFRWKVGSGKCISIDQDPWIDRRGIFSPI---------WVNDSLRGRLGCSIRK 464
           +++  QG R  VG+G+ I I +  W+D +   + +         + + S   ++   I +
Sbjct: 118 QEILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDE 177

Query: 465 GDGNWNEELIKEIFCPADAEAILNMPGGSTDLPDEIIWGYDSKGRFSVKSAYRLAVEVAS 524
               W +++I+ +F   + + I  +  G   + D   W Y S G ++VKS Y +  ++  
Sbjct: 178 SGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQI-- 237

Query: 525 LKEASTSNHLAE---NPSWKKLWKSDIMPKAKIEVWRIINNAIP 557
           + + S+   ++E   NP ++K+WKS   PK +  +W+ ++N++P
Sbjct: 238 INKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLP 279

BLAST of Lcy05g018000 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.9e-16
Identity = 59/232 (25.43%), Postives = 112/232 (48.28%), Query Frame = 0

Query: 375 LKSKYFREGDFLRAPAESKASFTWRSIVWGRDLFVQGFRWKVGSGKCISIDQDPWIDRRG 434
           +K++YF++   L A    + S+ W S++ G  L  +G R  +G G+ I I  D  +D   
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

Query: 435 IFSPIWVNDSLRGRLGCSIRKGDGN---WNEELIKEIFCPADAEAILNMPGGSTDLPDEI 494
              P+   ++ +     ++ +  G+   W++  I +    +D   I  +    +  PD+I
Sbjct: 61  P-RPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 495 IWGYDSKGRFSVKSAYRLAVEVASLKEASTSNHLAENPSWKKLWKSDIMPKAKIEVWRII 554
           IW Y++ G ++V+S Y L     S    + +          ++W   IMPK K  +WR +
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRAL 180

Query: 555 NNAIPTQKNICARGIGVNPMAPKPSVES-CKGHASWSPP-PPSSWKLNSDAS 602
           + A+ T + +  RG+ ++P  P+   E+    HA ++ P    +W+L SD+S
Sbjct: 181 SQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRL-SDSS 230

BLAST of Lcy05g018000 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 77.8 bits (190), Expect = 4.0e-14
Identity = 35/86 (40.70%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 345 FSELNLFNQALLAKQSWRILRSPDSLVARVLKSKYFREGDFLRAPAESKASFTWRSIVWG 404
           F +L  FNQALLAKQS+RI+  P +L++R+L+S+YF     +     ++ S+ WRSI+ G
Sbjct: 59  FRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHG 118

Query: 405 RDLFVQGFRWKVGSGKCISIDQDPWI 431
           R+L  +G    +G G    +  D WI
Sbjct: 119 RELLSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lcy05g018000 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 73.2 bits (178), Expect = 9.8e-13
Identity = 28/86 (32.56%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 82  LANRLKRVLDSIISPTEFAFVPDRLITYNVIIGFECIHSLTQRRRGNEGYVAIKLDMSKA 141
           +  RLK ++ ++I P + +F+P R+ T N++   E +HS+ +R++G +G++ +KLD+ KA
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSM-RRKKGVKGWMLLKLDLEKA 60

Query: 142 YDRVEWVFIKKVMGAMGFNDGWIDKV 168
           YDR+ W +++  + + GF + W+ ++
Sbjct: 61  YDRIRWDYLEDTLISAGFPEVWLPEI 85

BLAST of Lcy05g018000 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-12
Identity = 33/66 (50.00%), Postives = 41/66 (62.12%), Query Frame = 0

Query: 179 LINGHPYPIEKPSRGLRQGDPLSPYLFLMCAEGLLALINREVSMFNLNGFKINRYCPPLT 238
           +ING P  +  PSRGLRQGDPLSPYLF++C E L  L  R      L G +++   P + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 239 HLFFAD 245
           HL FAD
Sbjct: 73  HLLFAD 78