Lcy05g015530 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g015530
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	O-methyltransferase family protein
Location	Chr05: 19673297 .. 19678107 (-)
RNA-Seq Expression	Lcy05g015530
Synteny	Lcy05g015530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCGCTTGCTTACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGGCACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGGACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACCAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGTTTGTTTATGTTATAGCCTTGGACTATGGGATTGGTTTCAATTATACTCTTGAACTTTCAAAAGTTTCATTTTAACCTTTTAAGTCTGGAGTTTGTTTCAAAACAACCCTTGAAGACATTTTTTTCGTTAAACTTATGGACGGAAAATGATGAGTTTGTATATGTGTATAATGCTTATGTGCAGGTATTTGAACATATACATGGAAAAAACAATAAACACTCATGCAAACACATCATTTTTCGTCCATAAATTTAACGGAGATCTCCACAAACTTCAAACTTTGAGGATTAAAATGAAACTAGGTATAATTGAAACCAACTCCATAGTTTAGAGGTATTTTTTTTGATAATTTAACCAATGCTAAAGTTAATAAACAACTTGTGTAGTTTAACTTTGAGGTGAAAATGCAGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTCATTGATGAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCAAAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGGTTTTAACCGATATACCATTACTCCTACTCGAACTATCCAGTCAGTCATTCAAGGGTTTCCTTGAGACCTTGACATCAATCAAAGAAAATGAAATTTGGATTTGATTTCCTCATAAGTACTTTTATTGTGTTATGTCTCGATTTCATGTATGTGTCGTTGGTCTGTCTTTCTCTTTTGATTCTAAAAGTTTGTAATAATGATTTTAAAAGTAAGAGCATGCATTTCTATTCAATATTATTATTAGAAAATTTGTGTTCAATGTTTTTCTTTGCATTAATGTTATTTGATACTAGGGATAATTGTATAGATTAAATTCGTATAATTTGTGAATTTTATAAATTATCTTCAATTTTAAGGTTTTTTTTTTTTTTTTTGAGAAAGTATCTTAAATTTTAAGTTAAAATTTACTTGGAACAAAATAGTATGATTGGTAAAGTAAACAAAATAGATAGGTATAATATTCGCGAATATCTCTTGATGGGTATATCTTTAATTTTTTTTTCTCCTCTGTGAATTGTAACATAAATTATATAACTTTTAAGTTTATTGCATTTGATTAGACTTTTCTAATTGCATTATTATCCCTTCTTTATTTTATATCATAGATAGACTTCAATCATAATTGTCATTATTGTGAATGAGGTGTTGTAGTCTATCAACGATTCAAAGTCGATTAGTGATAGTAGTGAGACTGCTACTAGCGGTACTAGCCTTGTACAAGAGATAGGCAACATGTTGTGAATGAAGTCTATCAATGATAGGCTTGACTGATGAAGCAATCTCAACAAATATAACATTCTATTTTGTTTTGTTTGATGTTTAGTTGATTCAATATTGAACTCACAAGAGTCTAAAAAAATCATCATTTCATCAACCAAAACTCCAGTAGTCTTGTAATTCTCATAACAATCTTTATCATTCCATTGTTGATAACTCACAAAATGAGCTATTTTTCCTGATATAAATTAGTTAGCCTTTGAGATTAAATTGGCCAAAATCATACACTTTTGTGTTTTTTTTTTTGTAGGAATTGAATAATTGGGATGAAATTGAGAAATTGGAGTTGTCCGAGTGTTCGGGAACAAAAAAGAAACACAAGACTTGAAGAAGAATGGATTTGGGAGCTGCAACAACCAAGCAACCCGAGAGCTCAGCGTCAAGATGCTGTTGCATACCTATTCCCAGCTGAAGTCCCGAGACCACAGCATCTCGACGCTGTGGTAAGAGCTTCTCGACGCTGTACAACTTCCTTTTCTGATTCACGCGGCAACAGGATAGTGTCTCGACGTTGTCGTACATTTTCTATAAATACAATACGATTTCACACGACCAAGAGGAGGGGAGGTCCTTTTGGAGAGATTTTAGCCCTAATTCTGACTAGAGACCTCTTGTGGGAGCTTGAGCAAGGACAAGCAGCGGGATTCAAAGCTAGAACGCGTGGAGATCGTGACGGGGACGTTCGGTTTCGACGACATTCAAGTTTTTCTCTTCTTTCCTTATCTTTAGTTAGTTTATGACTTTTGTTTCTAGCTTATATTGTAACGAGGATTTCATGAGTAGCTAGATCTTTAATTCTTAGGGATTGATGCAGCCCTTTGGGCGCTGTGGATTAATTCTTGGATGTATGTTTTGAATTCATTTATGCATATTCTAGTCTTAATGCTTTTGATTCATTGTCCATGAATTGAATGTTATTTTCTCTAAGTCTTGCTCGGGAGAGGGGATTTAGTTTAAAACTTGTTTTGCATAACTAAGATAATCTTAAGATCGAAAGTATAGTCTTAGAAATCGTTAAAAAGGATCAGACTTAAGGCCTTGCATGATAATAAATCTAAATAGGGATATTGAATTTATTGCATGTATGAGAATTAGTTATGCTCGGGAGAGATAACCATGATATTAGTTTGTCCTAAGATTTATGAATCTTTGCATATTAACCATTAAAGTAATTAATTCACAACCTCAATTGGTGAAATCAACTCCCTAAGAGTTCTTTCCATTTGAATTTCCATCTTTTAGATTTCTTTAGTTTTAATTGATTTTTCTCACTAAGTTTTGATCTCTAAATAAAATTGAACAAGATTAATCACGACAATTAATTAGCTAGAACAACCAATCCAGAGGACGACACTCGTTAATCACGAAAGCACTATATTAAAACTTGACTCGTACACTTGCGGTAGCCATTTTGCACATCAATTGTCCACCAGGACAAACAACTATTGGAAGCTTAAGCATAATTTCAAATTATTGGAAAAACACACACATAATAAAAAACATCAAACTAGTTCATTTGCTTGCTTATCATATAACCTTTGAAAGTTAAGGGATGAGTTACTTTGAGGTTGTTCGAGTTCATGTTTCTTAATCTTAGGTCGTTACTTAGCAAAAAGTTGGTCGTTTATGTTTGTTGTATAAGTGTTGATGTTATTTTTCTAAATCTTTGATTTTGTTAATTAGCTTCTCTAGTTCACTTGTTGAATAGGTTTTGAGCATGTGAGTGTAGCTTTTCTAAGTTCACTTGTTGGCTTTGTTTGAAGGTTTTCTAATGTGAGGTGGGGTACGTCTCAAAAATTTAGATTTTCTTTTTTGTACTAGTTCTTATGTGTGGAACTTTGGATAAGGAAAAACAAGATTGGTTCGTTATGCATGTTCATTATTTGTTTGATTTTGTAAGTTGAGGGATGGTTTACTTAATAGTTTGTTATATCTTACGTATGTTGGCTATAAGTTTGATGTTACACTTCTATCTTTTATTGTGTTAGTTCCTTTGCTTGTTTATTGTTTAACCTTTTGAAAGTTGAGGGATGAGTTACTTCGAGGTAGTTCGAGTTCATGTTTCTTAACCTTATGATATGAACCACCAAGAACATTAAAATTATTATTTATATTTAATTTAATATTCTGGTGTAATTTTATTGTTTTTCAATTTTGTCCTTTTTATGACTTTATCAAGTAGGGGTATTTTTGTCATTTTGTTATGATCAATGATGTGACATGCAGTTGGGATTTTGGGGAGCTATTTTAAAGCTTTGCCATTAGGTTGTACAGGGGAGAACGATGAATTGAGATTTGAGCAATTGCGCTTTTCCCTTCAAGTTTAGCCAACAAAGCTTTTTGAATTTTGCAATTCATGTCTTGAGTTTTCATGCCAAGAACATTCAAGTAGGATTGATCATCCTATCTTGTGGAGTGTTCGACTCTTAGGTAAGGAACTTGTTCTTTGTCCGAAGAGCTTTGATCTTGAGGCAATCCGTGTCTAACCTAATCCTTAGGGTTGAAATCAAATCGATTTGAGGGTTTTAGCGTTGGTGAATTAAGGGTTCAAATACCCAAAGGATTCTTAATTCCAATTTGCTAGAGTTTTATTATCACCTTAGGTTACTTAGCAAAAAGTTGGTCGTTTATGTTCGTTGTATAAGTGTTGATGTTATTCTTCTAAAGCTGAATTCTTCTGACCTTGAGAGAACCGCTAGACCTCGCTATGATGTGGAGTTTTGGCTTGAATTGTTGTTTGTTGCATTATACTATAAAGAGGTGTTCAAATGCTTGAATTTGTAATCTCCTGTCACTTATCTATTTTGATATGCTCCAATGCGAAGGATTTGGGCGCATGACATGGGAAGTATTGGTCCTAAGAAAATCAACCACATCTTCTGGTGTCAGAGGCCAAAGTTCTGGTACATCGTTTCTAA

mRNA sequence

ATGTCTCGCTTGCTTACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGGCACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGGACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACCAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTCATTGATGAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCAAAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGAGGCCAAAGTTCTGGTACATCGTTTCTAA

Coding sequence (CDS)

ATGTCTCGCTTGCTTACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGGCACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGGACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACCAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTCATTGATGAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCAAAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGAGGCCAAAGTTCTGGTACATCGTTTCTAA

Protein sequence

MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKAEAKVLVHRF

Homology

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BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.0e-64
Identity = 125/244 (51.23%), Postives = 169/244 (69.26%), Query Frame = 0


Query: 4   LLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA--NGTT--PFEMAHGKDLWSYAAGD 63
           ++T     SLAP +L E +P MLAPW  LS+ + +  NG+T  PF+  HGKD+WS+A  +
Sbjct: 119 MITRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDN 178

Query: 64  PSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRG 123
           P  + ++NE MAC AR  ++P V   C  +F+GV ++VDVGGG G  + +LVK  PWI+G
Sbjct: 179 PFLSDMINEAMACDAR-RVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKG 238

Query: 124 INFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAI 183
            NFDLPHV+ V+    GV+++ G+MFD IP  DA FI  VLHDW D++CIKILKNCKEA+
Sbjct: 239 FNFDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAV 298

Query: 184 PEGTGKVIIVE---------VVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINV 235
           P   GKV+IVE         +++DE++E KL  VRLM+DM+MMAHT  GKERT +EW  V
Sbjct: 299 PPNIGKVLIVESVIGENKKTMIVDERDE-KLEHVRLMLDMVMMAHTSTGKERTLKEWDFV 358

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BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.7e-51
Identity = 109/238 (45.80%), Postives = 156/238 (65.55%), Query Frame = 0


Query: 2   SRLL----TSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAH-GKDLWSYA 61
           SRLL    T   + +LAP +     P + APW  L+  +K  G   FEM H G+ LW  +
Sbjct: 103 SRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLARCVKEGG-NGFEMVHGGRQLWDLS 162

Query: 62  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 121
            G+P  N + N+GMA  AR+  + A+L +  ++F G+ SLVDVGG  G  +S +VK+ P 
Sbjct: 163 PGNPEFNKVFNDGMASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPH 222

Query: 122 IRGINFDLPHVVSVSPEYKG-VQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNC 181
           I+GIN+DLPHVV+ +P Y G V H+GGNMF+ IP A A F+  +LHDW DE+C+KILKNC
Sbjct: 223 IKGINYDLPHVVATAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNC 282

Query: 182 KEAIPEGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQK 234
           + A+PE  GK+IIV++V++ +      D  +M+D+ +MA T +GKERT +EW  VL++
Sbjct: 283 RRAMPEKGGKIIIVDIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEE 337

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BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.3e-49
Identity = 103/236 (43.64%), Postives = 145/236 (61.44%), Query Frame = 0


Query: 2   SRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPS 61
           ++ L  G +  +   +L       +APWH L   L     T FE A G ++W Y A  P 
Sbjct: 95  AKYLVKGWDKCMVGSILAITDKDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPE 154

Query: 62  HNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGIN 121
            N L NE MA  +R+ I+ A++++CG IF G+ +LVDVGGG GT +  +  A P I+   
Sbjct: 155 KNQLFNEAMANDSRL-IMSALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTV 214

Query: 122 FDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPE 181
           +DLPHV++ SP Y  V  + G+MF  IPKADA  +  +LHDWDD+ECI+ILK CKEA+P 
Sbjct: 215 YDLPHVIADSPGYSEVHCVAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPV 274

Query: 182 GTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKAEAK 238
             GKVIIV++V++ + E   + +RL +D+ MM +T  GKERT EEW  ++  A  K
Sbjct: 275 KGGKVIIVDIVLNVQSEHPYTKMRLTLDLDMMLNT-GGKERTEEEWKKLIHDAGYK 328

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BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-49
Identity = 96/231 (41.56%), Postives = 145/231 (62.77%), Query Frame = 0


Query: 4   LLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPSHN 63
           LL    E S+ P++L       +  WH +   L    TT FE   G D+W Y  G+P  +
Sbjct: 108 LLLRDAERSMVPMILGMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQS 167

Query: 64  MLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFD 123
            L NEGMA   R+ +   ++E C + F+G+ SLVD+GGGNGT +  + +A P I+   +D
Sbjct: 168 QLFNEGMAGETRL-LTKTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYD 227

Query: 124 LPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGT 183
           LPHVV+ S +   ++ + G+MF  +P A A  +  +LHDW DEEC+ ILK CKEAIP+ T
Sbjct: 228 LPHVVANSHDLPNIEKVPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKET 287

Query: 184 GKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           GKVIIV+V ++E+   +L+  RL++D+ M+ +T  G+ERT ++W N+L++A
Sbjct: 288 GKVIIVDVALEEESNHELTKTRLILDIDMLVNT-GGRERTADDWENLLKRA 336

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BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.6e-49
Identity = 104/235 (44.26%), Postives = 148/235 (62.98%), Query Frame = 0


Query: 2   SRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPS 61
           S+ L    + +L+P++L E +P++L PW  L    +   ++PFE AHG ++W  A  DP 
Sbjct: 101 SKWLLKDSKFNLSPLVLWETNPILLKPWQYLGKCAQEK-SSPFERAHGCEIWDLALADPK 160

Query: 62  HNMLVNEGMACFARVEILPAVLEKCGEIFEGV-GSLVDVGGGNGTCLSILVKACPWIRGI 121
            N  +N  M C +   I+  +L +  + F G+ GSLVDVGGG G+ ++ +VKA P I+GI
Sbjct: 161 FNNFLNGAMQC-STTTIINEMLLEYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGI 220

Query: 122 NFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAI- 181
           NFDLPHVV+ + E+ GV+H+GG+MF  IP+ADA  +  +LHDW DE+C  ILKNC  AI 
Sbjct: 221 NFDLPHVVATAAEFPGVKHVGGDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIR 280

Query: 182 PEGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
            +  GKVIIV+ V+          + L+ D++MMAHT  GKERT  EW  +L  A
Sbjct: 281 KKKNGKVIIVDCVLRPDGNDLFDKMGLIFDVLMMAHTTAGKERTEAEWKILLNNA 333

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BLAST of Lcy05g015530 vs. ExPASy TrEMBL
Match: A0A6J1C1G3 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007584 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-109
Identity = 192/234 (82.05%), Postives = 213/234 (91.03%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDP 60
           MSRLLTS  ++S+AP+LLLE +PVMLAPWH LSAFLK + T PFE AHG+DLWSYAA DP
Sbjct: 99  MSRLLTSSSKSSMAPLLLLESNPVMLAPWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADP 158

Query: 61  SHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGI 120
           S  ML+NE MACFAR+ I+PAV+E+CGEIFEGVGSLVDVGGGNGTCLSILVKA PWIRGI
Sbjct: 159 SFTMLLNEAMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGI 218

Query: 121 NFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIP 180
           NFDLPHVV VSP Y+GV+H+GGNMFDC+PKADAAFIMEVLHDWDDEECIK+LKNCKEAIP
Sbjct: 219 NFDLPHVVLVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIP 278

Query: 181 EGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           + TGKVIIVE+VIDEKE++KL DVRLMMDMIMMAHT KGKERTREEW  VLQKA
Sbjct: 279 KKTGKVIIVEIVIDEKEQNKLLDVRLMMDMIMMAHTNKGKERTREEWAYVLQKA 332

[Close]

BLAST of Lcy05g015530 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.1e-108
Identity = 196/239 (82.01%), Postives = 214/239 (89.54%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+AP+ LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NE MACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLINESMACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+G+QH+GGNMFD +PKADAAFIMEVLHDWDDEECIKILKNCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE-SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVEVVIDEKEE SK SD RLMMDMIMMAHT KGKERT EEW NVLQKA
Sbjct: 280 EAIPEKTGKVIIVEVVIDEKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKA 338

[Close]

BLAST of Lcy05g015530 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.2e-106
Identity = 193/240 (80.42%), Postives = 215/240 (89.58%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+API LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NEGMACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLLNEGMACFARLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE--SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVE+VIDEKE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKA
Sbjct: 280 EAIPEKTGKVIIVEIVIDEKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKA 339

[Close]

BLAST of Lcy05g015530 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.2e-106
Identity = 193/240 (80.42%), Postives = 215/240 (89.58%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+API LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NEGMACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLLNEGMACFARLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE--SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVE+VIDEKE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKA
Sbjct: 280 EAIPEKTGKVIIVEIVIDEKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKA 339

[Close]

BLAST of Lcy05g015530 vs. ExPASy TrEMBL
Match: A0A6J1HVJ9 (xanthohumol 4'-O-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.7e-105
Identity = 192/236 (81.36%), Postives = 211/236 (89.41%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKAN--GTTPFEMAHGKDLWSYAAG 60
           MSRLLTS G +SLAPI +LE S VMLAPWHNLSA+LKAN  GT PFEMAHGKDLWSY A 
Sbjct: 65  MSRLLTSSGAHSLAPIHVLESSLVMLAPWHNLSAYLKANNGGTQPFEMAHGKDLWSYCAA 124

Query: 61  DPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIR 120
           + SHNM++NEGMA F+RV I+PAVLE+CG +FEGVGSLVDVGGGNG+CLS+LVKA PWIR
Sbjct: 125 NSSHNMVMNEGMASFSRVVIVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIR 184

Query: 121 GINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEA 180
           GINFDLPHVVSVS EY+GV+H+GGNMFD +PKADAAFIM  LHDWDDEECIKILKNCKEA
Sbjct: 185 GINFDLPHVVSVSQEYEGVRHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEA 244

Query: 181 IPEGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           IPE TGKVIIVEVVIDEKEES +SDVRLM+DMIM+AHT KGKERTREEW  VLQ A
Sbjct: 245 IPEKTGKVIIVEVVIDEKEESMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGA 300

[Close]

BLAST of Lcy05g015530 vs. NCBI nr
Match: XP_022135686.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 404.8 bits (1039), Expect = 5.1e-109
Identity = 192/234 (82.05%), Postives = 213/234 (91.03%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDP 60
           MSRLLTS  ++S+AP+LLLE +PVMLAPWH LSAFLK + T PFE AHG+DLWSYAA DP
Sbjct: 99  MSRLLTSSSKSSMAPLLLLESNPVMLAPWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADP 158

Query: 61  SHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGI 120
           S  ML+NE MACFAR+ I+PAV+E+CGEIFEGVGSLVDVGGGNGTCLSILVKA PWIRGI
Sbjct: 159 SFTMLLNEAMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGI 218

Query: 121 NFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIP 180
           NFDLPHVV VSP Y+GV+H+GGNMFDC+PKADAAFIMEVLHDWDDEECIK+LKNCKEAIP
Sbjct: 219 NFDLPHVVLVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIP 278

Query: 181 EGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           + TGKVIIVE+VIDEKE++KL DVRLMMDMIMMAHT KGKERTREEW  VLQKA
Sbjct: 279 KKTGKVIIVEIVIDEKEQNKLLDVRLMMDMIMMAHTNKGKERTREEWAYVLQKA 332

[Close]

BLAST of Lcy05g015530 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 401.7 bits (1031), Expect = 4.4e-108
Identity = 196/239 (82.01%), Postives = 214/239 (89.54%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+AP+ LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPLHLLESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NE MACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLINESMACFARLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+G+QH+GGNMFD +PKADAAFIMEVLHDWDDEECIKILKNCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE-SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVEVVIDEKEE SK SD RLMMDMIMMAHT KGKERT EEW NVLQKA
Sbjct: 280 EAIPEKTGKVIIVEVVIDEKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKA 338

[Close]

BLAST of Lcy05g015530 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 396.0 bits (1016), Expect = 2.4e-106
Identity = 193/240 (80.42%), Postives = 215/240 (89.58%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+API LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NEGMACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLLNEGMACFARLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE--SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVE+VIDEKE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKA
Sbjct: 280 EAIPEKTGKVIIVEIVIDEKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKA 339

[Close]

BLAST of Lcy05g015530 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 396.0 bits (1016), Expect = 2.4e-106
Identity = 193/240 (80.42%), Postives = 215/240 (89.58%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA----NGTTPFEMAHGKDLWSYA 60
           MSRLLT+ G NS+API LLE SPVM+APWHNLSA +KA    NG+ PFEMAHG DLW+YA
Sbjct: 100 MSRLLTTTGPNSMAPIHLLESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYA 159

Query: 61  AGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPW 120
           A +PSH+ L+NEGMACFAR+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPW
Sbjct: 160 AANPSHSSLLNEGMACFARLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPW 219

Query: 121 IRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCK 180
           I+GINFDLPHVVSVS EY+GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCK
Sbjct: 220 IKGINFDLPHVVSVSKEYEGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCK 279

Query: 181 EAIPEGTGKVIIVEVVIDEKEE--SKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           EAIPE TGKVIIVE+VIDEKE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKA
Sbjct: 280 EAIPEKTGKVIIVEIVIDEKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKA 339

[Close]

BLAST of Lcy05g015530 vs. NCBI nr
Match: XP_022969152.1 (xanthohumol 4'-O-methyltransferase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 391.0 bits (1003), Expect = 7.7e-105
Identity = 192/236 (81.36%), Postives = 211/236 (89.41%), Query Frame = 0


Query: 1   MSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKAN--GTTPFEMAHGKDLWSYAAG 60
           MSRLLTS G +SLAPI +LE S VMLAPWHNLSA+LKAN  GT PFEMAHGKDLWSY A 
Sbjct: 65  MSRLLTSSGAHSLAPIHVLESSLVMLAPWHNLSAYLKANNGGTQPFEMAHGKDLWSYCAA 124

Query: 61  DPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIR 120
           + SHNM++NEGMA F+RV I+PAVLE+CG +FEGVGSLVDVGGGNG+CLS+LVKA PWIR
Sbjct: 125 NSSHNMVMNEGMASFSRVVIVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIR 184

Query: 121 GINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEA 180
           GINFDLPHVVSVS EY+GV+H+GGNMFD +PKADAAFIM  LHDWDDEECIKILKNCKEA
Sbjct: 185 GINFDLPHVVSVSQEYEGVRHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEA 244

Query: 181 IPEGTGKVIIVEVVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINVLQKA 235
           IPE TGKVIIVEVVIDEKEES +SDVRLM+DMIM+AHT KGKERTREEW  VLQ A
Sbjct: 245 IPEKTGKVIIVEVVIDEKEESMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGA 300

[Close]

BLAST of Lcy05g015530 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 246.1 bits (627), Expect = 2.8e-65
Identity = 125/244 (51.23%), Postives = 169/244 (69.26%), Query Frame = 0


Query: 4   LLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKA--NGTT--PFEMAHGKDLWSYAAGD 63
           ++T     SLAP +L E +P MLAPW  LS+ + +  NG+T  PF+  HGKD+WS+A  +
Sbjct: 119 MITRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDN 178

Query: 64  PSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRG 123
           P  + ++NE MAC AR  ++P V   C  +F+GV ++VDVGGG G  + +LVK  PWI+G
Sbjct: 179 PFLSDMINEAMACDAR-RVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKG 238

Query: 124 INFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAI 183
            NFDLPHV+ V+    GV+++ G+MFD IP  DA FI  VLHDW D++CIKILKNCKEA+
Sbjct: 239 FNFDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAV 298

Query: 184 PEGTGKVIIVE---------VVIDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINV 235
           P   GKV+IVE         +++DE++E KL  VRLM+DM+MMAHT  GKERT +EW  V
Sbjct: 299 PPNIGKVLIVESVIGENKKTMIVDERDE-KLEHVRLMLDMVMMAHTSTGKERTLKEWDFV 358

[Close]

BLAST of Lcy05g015530 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 211.8 bits (538), Expect = 6.0e-55
Identity = 104/195 (53.33%), Postives = 134/195 (68.72%), Query Frame = 0


Query: 48  HGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCL 107
           HGKDLW++A  +  H+ L+NE MAC AR  ++P V   C  +F+GV ++VDVGGG G  +
Sbjct: 110 HGKDLWAFAQDNLCHSQLINEAMACDAR-RVVPRVAGACQGLFDGVATVVDVGGGTGETM 169

Query: 108 SILVKACPWIRGINFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEE 167
            ILVK  PWI+G NFDLPHV+ V+    GV+++ G+MFD IP +DA  I  VLHDW D++
Sbjct: 170 GILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDAVIIKWVLHDWGDKD 229

Query: 168 CIKILKNCKEAIPEGTGKVIIVEVVIDEKE--------ESKLSDVRLMMDMIMMAHTKKG 227
           CIKILKNCKEA+    GKV+IVE VI EK+        + KL  VRL +DM+MM HT  G
Sbjct: 230 CIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTG 289

Query: 228 KERTREEWINVLQKA 235
           KERT +EW  VL +A
Sbjct: 290 KERTLKEWDFVLTEA 303

[Close]

BLAST of Lcy05g015530 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-44
Identity = 90/217 (41.47%), Postives = 137/217 (63.13%), Query Frame = 0


Query: 12  SLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMA 71
           S+A + L+    V++  W++L   +  +G  PF  A+G   + Y   DP  N + N GM+
Sbjct: 121 SIAALCLMNQDKVLMESWYHLKDAI-LDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMS 180

Query: 72  CFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVS 131
             + +  +  +LE   + FEG+ SLVDVGGG G  L ++V   P ++GINFDLPHV+  +
Sbjct: 181 NHSTI-TMKKILETY-KGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDA 240

Query: 132 PEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEV 191
           P + G++H+GG+MF  +PK DA F+  + HDW DE C+K LKNC E++PE  GKVI+ E 
Sbjct: 241 PSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPE-DGKVILAEC 300

Query: 192 VIDEKEESKLSDVRLM-MDMIMMAHTKKGKERTREEW 228
           ++ E  +S LS  +++ +D IM+AH   GKERT +E+
Sbjct: 301 ILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEF 333

[Close]

BLAST of Lcy05g015530 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 159.8 bits (403), Expect = 2.7e-39
Identity = 93/229 (40.61%), Postives = 136/229 (59.39%), Query Frame = 0


Query: 4   LLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPSHN 63
           L  S G  SL+ +LLL  S V+L  W NL   +   G   F  AH   L+ Y + D   +
Sbjct: 132 LKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVI-LEGKDAFSSAHDMRLFEYISSDDQFS 191

Query: 64  MLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFD 123
            L +  M+  +   ++  VLE+    FE V +LVDVGGG GT L ++    P I+G+NFD
Sbjct: 192 KLFHRAMS-ESSTMVMKKVLEEY-RGFEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFD 251

Query: 124 LPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGT 183
           L  V++ +P Y GV+H+ G+MF  +PK DA F+  +LHDW DE+CIKILKNC +++PE  
Sbjct: 252 LAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPE-K 311

Query: 184 GKVIIVEVV--IDEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINV 231
           GKVIIVE++  ++ K      +  L MD++M+     GKER+  ++ N+
Sbjct: 312 GKVIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENL 356

[Close]

BLAST of Lcy05g015530 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 159.5 bits (402), Expect = 3.5e-39
Identity = 91/232 (39.22%), Postives = 131/232 (56.47%), Query Frame = 0


Query: 4   LLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANGTTPFEMAHGKDLWSYAAGDPSHN 63
           L  S G  SL P+ +L  + V    W NL   +   G   F  AHG  ++ Y   D    
Sbjct: 132 LRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVI-LEGRDAFNSAHGMKIFEYINSDQPFA 191

Query: 64  MLVNEGMACFARVEILPAVLEKCGEI---FEGVGSLVDVGGGNGTCLSILVKACPWIRGI 123
            L N  M+     E    +++K  ++   FE V +LVDVGGGNGT L ++    P I+G+
Sbjct: 192 ELFNRAMS-----EPSTMIMKKVLDVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPHIKGV 251

Query: 124 NFDLPHVVSVSPEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIP 183
           NFDL  V++ +P Y GV+H+ G+MF  +PK DA F+  +LHDW DE+CIKILKNC +++P
Sbjct: 252 NFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLP 311

Query: 184 EGTGKVIIVEVVI--DEKEESKLSDVRLMMDMIMMAHTKKGKERTREEWINV 231
           E  GK+IIVE V   + K     S+    MD++M+     GKER+  ++ N+
Sbjct: 312 E-KGKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENL 356

The following BLAST results are available for this feature:

BLAST of Lcy05g015530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	4.0e-64	51.23	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
B0ZB56	1.7e-51	45.80	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
Q9LEL6	1.3e-49	43.64	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
Q7XB10	2.1e-49	41.56	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu...	[more]
Q6WUC2	3.6e-49	44.26	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]

BLAST of Lcy05g015530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1G3	2.5e-109	82.05	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A0A0K0Q2	2.1e-108	82.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1	[more]
A0A5D3DI94	1.2e-106	80.42	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BHY1	1.2e-106	80.42	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1HVJ9	3.7e-105	81.36	xanthohumol 4'-O-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Lcy05g015530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135686.1	5.1e-109	82.05	(R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia]	[more]
XP_004144871.1	4.4e-108	82.01	acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical p...	[more]
KAA0040348.1	2.4e-106	80.42	(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233...	[more]
XP_008447905.1	2.4e-106	80.42	PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo]	[more]
XP_022969152.1	7.7e-105	81.36	xanthohumol 4'-O-methyltransferase-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Lcy05g015530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	2.8e-65	51.23	O-methyltransferase family protein	[more]
AT4G35150.1	6.0e-55	53.33	O-methyltransferase family protein	[more]
AT5G54160.1	3.6e-44	41.47	O-methyltransferase 1	[more]
AT1G77530.1	2.7e-39	40.61	O-methyltransferase family protein	[more]
AT1G77520.1	3.5e-39	39.22	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 217..237
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 7..242 e-value: 4.8E-73 score: 247.2
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 1..234
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 29..234 e-value: 1.1E-51 score: 175.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 1..234
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..243 score: 56.594425
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 12..234

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g015530.1	Lcy05g015530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity