Lcy05g015520 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g015520 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.2e-95
Identity = 170/360 (47.22%), Postives = 239/360 (66.39%), Query Frame = 0

Query: 5   HKESKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGS--PMTLSQLSSTLN 64
           +K    +E+ +A + +WKY+F F ++AA KCAI+LKI + IE + S  P+TL++LSS ++
Sbjct: 18  NKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVS 77

Query: 65  CSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSR--LLATNGENNMAPFLLLESS 124
            S   L RI+RFLVH+GIF+E    D   TGY  TPLSR  ++      ++APF+L E++
Sbjct: 78  ASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETT 137

Query: 125 PVMLAPWHNL----SVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVT 184
           P MLAPW  L    S  V  S   PF+A HG+DVWS+A++NP  S +INE MAC    V 
Sbjct: 138 PEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVV 197

Query: 185 CAILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQH 244
             +   C  +F+GV ++VDVGGG G  + +LVK  PW++G NFDLPHV+  +     V++
Sbjct: 198 PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVEN 257

Query: 245 VAGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVI------ 304
           V G+MFD +P  D  F+KWVLHDW D++CI ILKNCKEA+P N G+V+I+E+VI      
Sbjct: 258 VEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKT 317

Query: 305 ---DEKEDNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVI 348
              DE+++    +RLMLD+VMMAHT  GKERT +EW +++   GF+RY +  I  +QS+I
Sbjct: 318 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Lcy05g015520 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.6e-76
Identity = 146/348 (41.95%), Postives = 218/348 (62.64%), Query Frame = 0

Query: 14  GQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLSSTLNCSSP------- 73
           GQA  ++W+++F+F +  A+KCA+EL I D I ++G P+T+S++  +L  ++P       
Sbjct: 10  GQA--EIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSPNID 69

Query: 74  LLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLESSPVMLAP 133
            L RI+R LVH+ +F  E   + N   Y  T  S+ L  + + N++P +L E++P++L P
Sbjct: 70  YLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPILLKP 129

Query: 134 WHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCAILEGCPDI 193
           W  L    +   S PFE AHG ++W  A  +PK +  +N  M CS   +   +L    D 
Sbjct: 130 WQYLGKCAQEKSS-PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEYKDG 189

Query: 194 FNGV-GSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVAGNMFDFV 253
           F+G+ GSLVDVGGG G+I++ +VKA P ++GINFDLPHVV+T+ E+  V+HV G+MF  +
Sbjct: 190 FSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMFVDI 249

Query: 254 PNADVAFLKWVLHDWNDEECINILKNCKEAI-PKNTGRVIIIEAVI-DEKEDNFSDIRLM 313
           P AD   +KW+LHDW+DE+C  ILKNC  AI  K  G+VII++ V+  +  D F  + L+
Sbjct: 250 PEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKMGLI 309

Query: 314 LDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            D++MMAHT  GKERT  EW  ++N  GF RY +    A   +I+ FP
Sbjct: 310 FDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of Lcy05g015520 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 139/337 (41.25%), Postives = 209/337 (62.02%), Query Frame = 0

Query: 19  QMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLSSTL---NCSSPLLHRILRFL 78
           Q+W  I+ F +   ++CA+E+ IAD I+     +TL+QL++ L   N SS  L+R++R+L
Sbjct: 22  QLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVRYL 81

Query: 79  VHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLESSPVMLAPWHNLSVHVK 138
           VH  I  +ET        Y+  P+  LL  + E +M P +L  +    +  WH +   + 
Sbjct: 82  VHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEGLG 141

Query: 139 NSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCAILEGCPDIFNGVGSLVD 198
           N  +  FE   G D+W Y E NP  S+L NEGMA    ++T  ++E C D F G+ SLVD
Sbjct: 142 NGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQGLDSLVD 201

Query: 199 VGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVAGNMFDFVPNADVAFLKW 258
           +GGGNGT +  + +A P ++   +DLPHVV+ S +  N++ V G+MF  VP+A    LK 
Sbjct: 202 IGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAILLKL 261

Query: 259 VLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKEDN-FSDIRLMLDLVMMAHTKK 318
           +LHDW DEEC+NILK CKEAIPK TG+VII++  ++E+ ++  +  RL+LD+ M+ +T  
Sbjct: 262 ILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVNT-G 321

Query: 319 GKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
           G+ERT ++W  ++   GF  + I PI+AIQSVI+ FP
Sbjct: 322 GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

BLAST of Lcy05g015520 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 5.2e-73
Identity = 149/351 (42.45%), Postives = 221/351 (62.96%), Query Frame = 0

Query: 8   SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLSSTLNCSSPL 67
           SK ++  QA  ++WK I+ F E   +KCA++L+IA+T+     PM+LS+L+S L  + P+
Sbjct: 5   SKIDQQNQA--KIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPV 64

Query: 68  ----LHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLESSPVM 127
               L RI+R+LVH  +F+     D     Y+  P ++ L    E +M   +L  +    
Sbjct: 65  NEDRLFRIMRYLVHMELFK----IDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDF 124

Query: 128 LAPWHNLSVHVK-NSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCAILEG 187
           LAPWH+L   +  N D+  FE A G+ +W Y   NP+ ++L N  MAC   +VT A+   
Sbjct: 125 LAPWHHLGDGLTGNCDA--FEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANE 184

Query: 188 CPDIF-NGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVAGNM 247
           C  IF +G+ +LVDVGGG GT +  + KA P ++   +DLPHV++ SPE  N+  ++G+M
Sbjct: 185 CKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDM 244

Query: 248 FDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVID-EKEDNFSDI 307
           F  +P+AD  F+K +LHDWND+ECI ILK CKEA+PK  G+VII++ VID +    ++ I
Sbjct: 245 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPYAKI 304

Query: 308 RLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
           RL LDL MM +T  GKERT+EEW  + +A GF+ + +T I A+QSVI+ +P
Sbjct: 305 RLTLDLDMMLNT-GGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

BLAST of Lcy05g015520 vs. ExPASy Swiss-Prot
Match: Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 146/357 (40.90%), Postives = 216/357 (60.50%), Query Frame = 0

Query: 1   MEFSHKESKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLSST 60
           M F  K+   +   QA   +WK I+ F +   ++CA+EL I D I+    PM L+ L+S 
Sbjct: 1   MAFHGKDDVLDIKAQA--HVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASK 60

Query: 61  L-----NCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFL 120
           L     NC +  L+RILR+LV   I R E  +D     YA  P++ LL+ N + +M P +
Sbjct: 61  LPVSDVNCDN--LYRILRYLVKMEILRVEK-SDDGQKKYALEPIATLLSRNAKRSMVPMI 120

Query: 121 LLESSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMV 180
           L  +    + PWH++   + ++ +  FE A G  +W Y E +P  S+L NEGMA    ++
Sbjct: 121 LGMTQKDFMTPWHSMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLL 180

Query: 181 TCAILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQ 240
           T +++ G  D+F G+ SLVDVGGGNGT +  +  A P ++   FDLPHV++ S +  N++
Sbjct: 181 TSSLISGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIE 240

Query: 241 HVAGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKED 300
            + G+MF  VP+A    LK +LHDWNDE+ I ILK C+ A+PK+ G+VII++  +DE+ D
Sbjct: 241 RIGGDMFKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESD 300

Query: 301 N-FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
           +  S  RL+LD+ M+ +T  GKERT+E W  I+ + GFS   I  I AIQSVI+ FP
Sbjct: 301 HELSSTRLILDIDMLVNT-GGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350

BLAST of Lcy05g015520 vs. ExPASy TrEMBL
Match: A0A6J1EN16 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111435052 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.2e-151
Identity = 265/355 (74.65%), Postives = 304/355 (85.63%), Query Frame = 0

Query: 1   MEFSHKES---KKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQL 60
           ME + KE+   K+EEDGQAIIQMWKYIF F+EMAAIKCAIEL+IAD IE +GSPMTL QL
Sbjct: 1   MEGTEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLL 120
           SSTL+CSSPLLHRILRFLV RGIF+E +I+D +L GYAQTPLSRLLAT   +++APF+LL
Sbjct: 61  SSTLHCSSPLLHRILRFLVRRGIFKEYSISD-DLIGYAQTPLSRLLATKNGSSLAPFMLL 120

Query: 121 ESSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTC 180
           E+SP MLA WHNLS HVK+   FPFEAAHG DVW YA  NPKH++LINE M CS  +V  
Sbjct: 121 EASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVA 180

Query: 181 AILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHV 240
           AIL+GC  IF+GVGSLVDVGGGNG  LS++VKAC W+RGINFDLPHVVSTSP Y+ V+HV
Sbjct: 181 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVVSTSPVYERVEHV 240

Query: 241 AGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKED-N 300
           AG+MFDFVPNAD AF+KW LHDWNDEECI ILKNCKEAIPKNTG+VII++AVIDEKE+  
Sbjct: 241 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETE 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LMLDLVMMAHT  GKERTR+EW Y+INA GFSR+T+T IQAIQSVI+CFP
Sbjct: 301 QSDVGLMLDLVMMAHTSTGKERTRKEWAYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Lcy05g015520 vs. ExPASy TrEMBL
Match: A0A5A7TGF8 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004180 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 259/359 (72.14%), Postives = 305/359 (84.96%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HKE  S+KEEDGQAIIQMW+YIF FTEMAAIKCAI+LKIAD IE+YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           STLNCSS LL+RILRFL+HRGIF+ ETI D+N  GY+QTP+SRLLATN EN+MAP LLLE
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETI-DENQIGYSQTPMSRLLATNVENSMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLAPW +LS H+KNSD+ PFE AHG+D+W+YAE N +H+ L NE MACS  ++  A
Sbjct: 121 TSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           I+EGC D+F+GVG LVDVGGGNG+ L+ILVK CPWM+GINFDLPHVV  SP+Y+NV+HVA
Sbjct: 181 IIEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKE---- 300
           GNMFDFVP+ADVAFLKW+LHDW DEECI ILK CKEAIPK+ G+VIIIEA+I E E    
Sbjct: 241 GNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEK 300

Query: 301 --DNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
                SD+ LM DLVMMAHT KG+ERT EEW ++I+  GF+R+TITP+QAIQS+IQCFP
Sbjct: 301 MKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of Lcy05g015520 vs. ExPASy TrEMBL
Match: A0A6J1JMC1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111486486 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 263/354 (74.29%), Postives = 303/354 (85.59%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME + KE  SKKEE+GQAIIQMWKYIF F+EMAAIKCAIEL+IAD IE +GSPMTL QLS
Sbjct: 1   MERTDKETLSKKEEEGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           STL+CSSPLLHRILRFLV RGIF+E +I+D +L GYAQTPLSRLLAT   +++AP +LLE
Sbjct: 61  STLHCSSPLLHRILRFLVSRGIFKEYSISD-DLIGYAQTPLSRLLATKNGSSLAPLMLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SP MLA WHNLS +VK+   FPFEAAHG DVW YA  NPKH++LINE M CS  +V  A
Sbjct: 121 ASPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           IL+GC  IF+GVGSLVDVGGGNG  LS++VKAC W+RGINFDLPHV+STSP Y+ V+HVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKED-NF 300
           G+MFDFVPNAD AF+KW LHDWNDEECI ILKNCKEAIPKNTG+VII++AVIDEKE+   
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
           SD+ LMLDLVMMAHT  GKERTR+EW Y+INAVGFSR+T+T IQAIQSVI+C P
Sbjct: 301 SDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 353

BLAST of Lcy05g015520 vs. ExPASy TrEMBL
Match: A0A1S3BJE6 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490250 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 6.1e-149
Identity = 258/359 (71.87%), Postives = 304/359 (84.68%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HKE  S+KEEDGQAIIQMW+YIF FTEMAAIKCAI+LKIAD IE+YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           STLNCSS LL+RILRFL+HRGIF+ ETI D+N  GY+QTP+SRLLA N EN+MAP LLLE
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETI-DENQIGYSQTPMSRLLAINVENSMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLAPW +LS H+KNSD+ PFE AHG+D+W+YAE N +H+ L NE MACS  ++  A
Sbjct: 121 TSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           I+EGC D+F+GVG LVDVGGGNG+ L+ILVK CPWM+GINFDLPHVV  SP+Y+NV+HVA
Sbjct: 181 IIEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKE---- 300
           GNMFDFVP+ADVAFLKW+LHDW DEECI ILK CKEAIPK+ G+VIIIEA+I E E    
Sbjct: 241 GNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEK 300

Query: 301 --DNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
                SD+ LM DLVMMAHT KG+ERT EEW ++I+  GF+R+TITP+QAIQS+IQCFP
Sbjct: 301 MKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of Lcy05g015520 vs. ExPASy TrEMBL
Match: A0A0A0K629 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.9e-148
Identity = 256/358 (71.51%), Postives = 303/358 (84.64%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HKE  S+KEEDGQAIIQMW+YIF FTEMAAIKC ++LKIAD IE+YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           STLNCSS LLHRILRFL+HRGIF++ETI D+N  GY+QTP+SRLLATN EN+MAP LLLE
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETI-DENQIGYSQTPMSRLLATNVENSMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLAPW +LS H+KNSD+ PFE AHG+D+W YAE N +H+ L NE MAC   M+  A
Sbjct: 121 TSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           I+EGC D+F+GV  LVDVGGGNG+ LSILVKA PWM+GINFDLPHVV  SP+Y+NV+HVA
Sbjct: 181 IIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVI-----DEK 300
           GNMFDFVPNADVAFLKW+LHDW+DEECI ILK CKE+IPK+ G+VIIIEA+I     ++K
Sbjct: 241 GNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKK 300

Query: 301 EDNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
               SD+ LM DLVMMAHT +GKERT +EW ++I+  GF+ +TITPIQAIQS+IQCFP
Sbjct: 301 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of Lcy05g015520 vs. NCBI nr
Match: XP_038887538.1 (acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-153
Identity = 267/355 (75.21%), Postives = 303/355 (85.35%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HKE  SK EEDGQAI+QMW+YIF FTEMAAIKC I+LKIAD IE+YGSPMTLS LS
Sbjct: 1   MEVKHKECSSKTEEDGQAIVQMWRYIFRFTEMAAIKCVIDLKIADIIESYGSPMTLSHLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           S LNCSS LLHRILRFL+HRGIF+EETI  +N T Y+QTPLSRLLATN E+ MAP LLLE
Sbjct: 61  SALNCSSSLLHRILRFLIHRGIFKEETIG-ENQTAYSQTPLSRLLATNVESTMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLA W NLS H+KNS++FPFE AHG+D+WSYAE NP+H+ L NE MAC   ++  A
Sbjct: 121 TSPVMLAAWPNLSAHLKNSETFPFEIAHGKDLWSYAEANPEHNVLFNEAMACYAKVIAFA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           ILEGC DIF+GVG LVDVGGGNG+ LSILV ACPWM+GINFDLPHVV  SP+Y+NVQHVA
Sbjct: 181 ILEGCGDIFDGVGCLVDVGGGNGSTLSILVNACPWMKGINFDLPHVVCASPQYENVQHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKEDN-- 300
           GNMFDFVP ADVAFLKW+LHDWNDEECI ILK CKEAIPK+ G+VII+EA+I+EK +N  
Sbjct: 241 GNMFDFVPKADVAFLKWILHDWNDEECIKILKRCKEAIPKSGGKVIIVEAIIEEKSENKK 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LM DLVMMAHT KGKERT EEW ++INA GF+RYTITPIQ IQS+IQCFP
Sbjct: 301 VSDVGLMFDLVMMAHTNKGKERTIEEWAFVINAAGFTRYTITPIQPIQSLIQCFP 354

BLAST of Lcy05g015520 vs. NCBI nr
Match: XP_038887354.1 (acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 552.4 bits (1422), Expect = 2.9e-153
Identity = 266/355 (74.93%), Postives = 303/355 (85.35%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HKE  SK EEDGQAI+QMW+YIF FTEMAAIKC I+LKIAD IE+YGSPMTLS LS
Sbjct: 1   MEVKHKECSSKTEEDGQAIVQMWRYIFRFTEMAAIKCVIDLKIADIIESYGSPMTLSHLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           S LNCSS +LHRILRFL+HRGIF+EETI  +N T Y+QTPLSRLLATN E+ MAP LLLE
Sbjct: 61  SALNCSSSILHRILRFLIHRGIFKEETIG-ENQTAYSQTPLSRLLATNVESTMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLA W NLS H+KNS++FPFE AHG+D+WSYAE NP+H+ L NE MAC   ++  A
Sbjct: 121 TSPVMLAAWPNLSAHLKNSETFPFEIAHGKDLWSYAEANPEHNVLFNEAMACYAKVIAFA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           ILEGC DIF+GVG LVDVGGGNG+ LSILV ACPWM+GINFDLPHVV  SP+Y+NVQHVA
Sbjct: 181 ILEGCGDIFDGVGCLVDVGGGNGSTLSILVNACPWMKGINFDLPHVVCASPQYENVQHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKEDN-- 300
           GNMFDFVP ADVAFLKW+LHDWNDEECI ILK CKEAIPK+ G+VII+EA+I+EK +N  
Sbjct: 241 GNMFDFVPKADVAFLKWILHDWNDEECIKILKRCKEAIPKSGGKVIIVEAIIEEKSENKK 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LM DLVMMAHT KGKERT EEW ++INA GF+RYTITPIQ IQS+IQCFP
Sbjct: 301 VSDVGLMFDLVMMAHTNKGKERTIEEWAFVINAAGFTRYTITPIQPIQSLIQCFP 354

BLAST of Lcy05g015520 vs. NCBI nr
Match: XP_038888809.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 547.4 bits (1409), Expect = 9.3e-152
Identity = 262/355 (73.80%), Postives = 303/355 (85.35%), Query Frame = 0

Query: 1   MEFSHKE--SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLS 60
           ME  HK+  SKKE+DGQAIIQMWKY+FSFTEMAAIKC I+LKIAD IE+YGSPMTLS LS
Sbjct: 1   MEVEHKKCSSKKEDDGQAIIQMWKYVFSFTEMAAIKCVIDLKIADIIESYGSPMTLSNLS 60

Query: 61  STLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLE 120
           STLNCSS LL+RILRFL+HRGIF+EETI  +N T Y+QTPLSRLLATN EN MAP LLLE
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKEETIG-ENQTVYSQTPLSRLLATNVENTMAPLLLLE 120

Query: 121 SSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCA 180
           +SPVMLA W NLS H+KNS++ PFE AHG D WSYAE NP+H+ L NE MAC   ++  A
Sbjct: 121 TSPVMLAAWPNLSAHLKNSETLPFEIAHGMDFWSYAEANPEHNVLFNEAMACYAKVIASA 180

Query: 181 ILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVA 240
           ILEGC D+F+GVG LVDVGGGNG+ LSILVKACPWM+GINFDLPHVV  +P+Y+NVQHVA
Sbjct: 181 ILEGCGDVFDGVGCLVDVGGGNGSTLSILVKACPWMKGINFDLPHVVCAAPQYENVQHVA 240

Query: 241 GNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEK--EDN 300
           GNMFDFVP ADVAFLKW+LHDWNDEECI ILK CKE IPK+ G+VII+EA+++EK  ++ 
Sbjct: 241 GNMFDFVPEADVAFLKWILHDWNDEECIKILKKCKEVIPKSGGKVIIVEAIVEEKGEKNK 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LM D+ MMAHT KGKERT EEW ++INA GF+RYTITPIQA+QS+IQCFP
Sbjct: 301 KSDVGLMFDVAMMAHTNKGKERTIEEWAFVINAAGFTRYTITPIQAVQSLIQCFP 354

BLAST of Lcy05g015520 vs. NCBI nr
Match: XP_022928138.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucurbita moschata] >XP_022928139.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 545.0 bits (1403), Expect = 4.6e-151
Identity = 265/355 (74.65%), Postives = 304/355 (85.63%), Query Frame = 0

Query: 1   MEFSHKES---KKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQL 60
           ME + KE+   K+EEDGQAIIQMWKYIF F+EMAAIKCAIEL+IAD IE +GSPMTL QL
Sbjct: 1   MEGTEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLL 120
           SSTL+CSSPLLHRILRFLV RGIF+E +I+D +L GYAQTPLSRLLAT   +++APF+LL
Sbjct: 61  SSTLHCSSPLLHRILRFLVRRGIFKEYSISD-DLIGYAQTPLSRLLATKNGSSLAPFMLL 120

Query: 121 ESSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTC 180
           E+SP MLA WHNLS HVK+   FPFEAAHG DVW YA  NPKH++LINE M CS  +V  
Sbjct: 121 EASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVA 180

Query: 181 AILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHV 240
           AIL+GC  IF+GVGSLVDVGGGNG  LS++VKAC W+RGINFDLPHVVSTSP Y+ V+HV
Sbjct: 181 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVVSTSPVYERVEHV 240

Query: 241 AGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKED-N 300
           AG+MFDFVPNAD AF+KW LHDWNDEECI ILKNCKEAIPKNTG+VII++AVIDEKE+  
Sbjct: 241 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETE 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LMLDLVMMAHT  GKERTR+EW Y+INA GFSR+T+T IQAIQSVI+CFP
Sbjct: 301 QSDVGLMLDLVMMAHTSTGKERTRKEWAYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Lcy05g015520 vs. NCBI nr
Match: KAG6589030.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.3 bits (1396), Expect = 3.0e-150
Identity = 265/355 (74.65%), Postives = 302/355 (85.07%), Query Frame = 0

Query: 1   MEFSHKES---KKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQL 60
           ME S KE+   K+EEDGQAIIQMWKYIF F+EMAAIKCAIEL+IAD IE +GSPMTL QL
Sbjct: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLL 120
           SSTLNCS PLLHRILRFLV RGIF+E +I+D +L GYAQTPLSRLLAT   +++APF+LL
Sbjct: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISD-DLIGYAQTPLSRLLATKNGSSLAPFMLL 120

Query: 121 ESSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTC 180
           E+SP MLA WHNLS HVK+   FPFEAAHG DVW YA  NPKH++LINE M CS  +V  
Sbjct: 121 EASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVA 180

Query: 181 AILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHV 240
           AIL+GC  IF+GVGSLVDVGGGNG  LS++VKAC W+RGINFDL HVVSTSP Y+ V+HV
Sbjct: 181 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHV 240

Query: 241 AGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVIDEKED-N 300
           AG+MFDFVPNAD AF+KW LHDWNDEECI ILKNCKEAIPKNTG+VII++AVIDEKE+  
Sbjct: 241 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETE 300

Query: 301 FSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVIQCFP 352
            SD+ LMLDLVMMAHT  GKERTR+EW Y+INA GFSR+T+T IQAIQSVI+CFP
Sbjct: 301 QSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Lcy05g015520 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.5e-96
Identity = 170/360 (47.22%), Postives = 239/360 (66.39%), Query Frame = 0

Query: 5   HKESKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGS--PMTLSQLSSTLN 64
           +K    +E+ +A + +WKY+F F ++AA KCAI+LKI + IE + S  P+TL++LSS ++
Sbjct: 18  NKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVS 77

Query: 65  CSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSR--LLATNGENNMAPFLLLESS 124
            S   L RI+RFLVH+GIF+E    D   TGY  TPLSR  ++      ++APF+L E++
Sbjct: 78  ASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETT 137

Query: 125 PVMLAPWHNL----SVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVT 184
           P MLAPW  L    S  V  S   PF+A HG+DVWS+A++NP  S +INE MAC    V 
Sbjct: 138 PEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVV 197

Query: 185 CAILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQH 244
             +   C  +F+GV ++VDVGGG G  + +LVK  PW++G NFDLPHV+  +     V++
Sbjct: 198 PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVEN 257

Query: 245 VAGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVI------ 304
           V G+MFD +P  D  F+KWVLHDW D++CI ILKNCKEA+P N G+V+I+E+VI      
Sbjct: 258 VEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKT 317

Query: 305 ---DEKEDNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVI 348
              DE+++    +RLMLD+VMMAHT  GKERT +EW +++   GF+RY +  I  +QS+I
Sbjct: 318 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Lcy05g015520 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.6e-80
Identity = 148/356 (41.57%), Postives = 213/356 (59.83%), Query Frame = 0

Query: 6   KESKK---EEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGS--PMTLSQLSST 65
           +ESK+   +E+ +A + +W+Y+F F ++AA KCAI+LKI + IE + S  P+TLS+LSS 
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 66  LNCSSPLLHRILRFLVHRGIFREETIADQNLTGYAQTPLSRLLATNGENNMAPFLLLESS 125
           ++ S   L RI+RFLVH+G+F+E    D   TGY  TPLSR +                 
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM---------------- 121

Query: 126 PVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCAIL 185
                                    HG+D+W++A++N  HS+LINE MAC    V   + 
Sbjct: 122 ---------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVA 181

Query: 186 EGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVAGN 245
             C  +F+GV ++VDVGGG G  + ILVK  PW++G NFDLPHV+  +     V++V G+
Sbjct: 182 GACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGD 241

Query: 246 MFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVI---------D 305
           MFD +P +D   +KWVLHDW D++CI ILKNCKEA+  N G+V+I+E VI         +
Sbjct: 242 MFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAE 301

Query: 306 EKEDNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITPIQAIQSVI 348
           E++D    +RL LD+VMM HT  GKERT +EW +++   GF+RY +     +QS+I
Sbjct: 302 ERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of Lcy05g015520 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 199.1 bits (505), Expect = 5.8e-51
Identity = 109/314 (34.71%), Postives = 178/314 (56.69%), Query Frame = 0

Query: 32  AIKCAIELKIADTIEAYGSPMTLSQLSSTLNCSSP----LLHRILRFLVHRGIFREETIA 91
           A+K A+EL + + +   GSPM+ ++++S L   +P    +L RILR L    +    T +
Sbjct: 35  ALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL---TCS 94

Query: 92  DQNLTG------YAQTPLSRLLATNGEN-NMAPFLLLESSPVMLAPWHNLSVHVKNSDSF 151
           ++ L+G      Y   P+ + L  N +  ++A   L+    V++  W++L   + +    
Sbjct: 95  NRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDG-GI 154

Query: 152 PFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVTCAILEGCPDIFNGVGSLVDVGGGN 211
           PF  A+G   + Y   +P+ +K+ N GM+    +    ILE     F G+ SLVDVGGG 
Sbjct: 155 PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGI 214

Query: 212 GTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQHVAGNMFDFVPNADVAFLKWVLHDW 271
           G  L ++V   P ++GINFDLPHV+  +P +  ++HV G+MF  VP  D  F+KW+ HDW
Sbjct: 215 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 274

Query: 272 NDEECINILKNCKEAIPKNTGRVIIIEAVIDEKEDNFSDIR--LMLDLVMMAHTKKGKER 331
           +DE C+  LKNC E++P++ G+VI+ E ++ E  D+    +  + +D +M+AH   GKER
Sbjct: 275 SDEHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 334

Query: 332 TREEWTYIINAVGF 333
           T +E+  +  A GF
Sbjct: 335 TEKEFEALAKASGF 342

BLAST of Lcy05g015520 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 121/369 (32.79%), Postives = 192/369 (52.03%), Query Frame = 0

Query: 6   KESKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKIADTIEAYGSPMTLSQLSSTLNCSS 65
           KE ++ ++    +Q    + +      +K A EL + DTI A G+   LS     + CS 
Sbjct: 19  KEEQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCE--IACSL 78

Query: 66  P----------LLHRILRFLVHRGIFREETI--ADQNLTG-----YAQTPLSR--LLATN 125
           P          LL R+L  LV   I +   I   +   TG     YA  P+ +  L  ++
Sbjct: 79  PTKPTNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSD 138

Query: 126 GENNMAPFLLLESSPVMLAPWHNL-SVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLIN 185
           G  ++ P  +L  + V    W NL  V ++  D+  F +AHG  ++ Y   +   ++L N
Sbjct: 139 GTGSLVPLFMLLHTQVFFKTWTNLKDVILEGRDA--FNSAHGMKIFEYINSDQPFAELFN 198

Query: 186 EGMACSGAMVTCAILEGCPDIFNG---VGSLVDVGGGNGTILSILVKACPWMRGINFDLP 245
             M+    M+   +L    D++ G   V +LVDVGGGNGT+L ++    P ++G+NFDL 
Sbjct: 199 RAMSEPSTMIMKKVL----DVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLA 258

Query: 246 HVVSTSPEYKNVQHVAGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGR 305
            V++ +P Y  V+HV+G+MF  VP  D  F+KW+LHDW DE+CI ILKNC +++P+  G+
Sbjct: 259 QVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GK 318

Query: 306 VIIIEAVIDEKE---DNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGFSRYTITP 349
           +II+E V  ++    D  S+    +DL+M+     GKER+  ++  +  A GF R  I  
Sbjct: 319 IIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIIC 378

BLAST of Lcy05g015520 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.5e-46
Identity = 112/339 (33.04%), Postives = 186/339 (54.87%), Query Frame = 0

Query: 8   SKKEEDGQAIIQMWKYIFSFTEMAAIKCAIELKI------ADTIEAYGSPMTLSQLSSTL 67
           S +EED    IQ+    F       +K A EL +      A  + +Y SP+ L+ +++  
Sbjct: 10  SSEEEDMLLAIQLGGLNF---VPYIVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPK 69

Query: 68  NCSSP-LLHRILRFLVHRGIFREETIADQ---NLTGYAQTPLSRLLATNGEN-NMAPFLL 127
           N  +P ++ R+LRFLV   +   + + D+       Y    + + L  + +  ++AP++L
Sbjct: 70  NPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVL 129

Query: 128 LESSPVMLAPWHNLSVHVKNSDSFPFEAAHGQDVWSYAEENPKHSKLINEGMACSGAMVT 187
              +      W+N+   ++   +  +E A+   ++ Y ++N    K+ NE M    ++V 
Sbjct: 130 AGCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVM 189

Query: 188 CAILEGCPDIFNGVGSLVDVGGGNGTILSILVKACPWMRGINFDLPHVVSTSPEYKNVQH 247
             ILE     F GV   VDVGG  G+ L+ ++   P ++GINFDLPH+V  +P+   V+H
Sbjct: 190 KKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEH 249

Query: 248 VAGNMFDFVPNADVAFLKWVLHDWNDEECINILKNCKEAIPKNTGRVIIIEAVID---EK 307
           + G+MFD +P  +V  +KW+LHDWNDE+C+ ILKNCK+A+P+ TGR+I+IE ++     +
Sbjct: 250 IGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSE 309

Query: 308 EDNFSDIRLMLDLVMMAHTKKGKERTREEWTYIINAVGF 333
            D  +   L  DL MM+ T  GKERT++E+  +    GF
Sbjct: 310 TDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGF 343