Lcy05g012530 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g012530
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMethyl esterase 1
LocationChr05: 14502067 .. 14505336 (-)
RNA-Seq ExpressionLcy05g012530
SyntenyLcy05g012530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTGGAAGTCATCAGATCTAGTGTTGTCCGAAGAGGCTGAGGACTTGTCAGCGTTGAAGTCCATCACCGACTCCATCGTTGGCCTCGCTTCTCGACTCCCTTCGGTCGAATTCTGCCACTGTAGTAGAGCCTCGAACTCAATCGCCCACTGCGTTGCGAGGCAAGCCTGTAATTTGTCTTTTGTCGCTGACCAGGAAGTTCCCCTCCTGCGGGAAGTTGGACAGTTACGTTTTTTGTAGCCCCTGGTTTTTCATTTTGTTTTGGTGATTCTTTTGTTGAGAACATTTTGGTTGGTGGTTAGCTTTAATGAAGTATTTATTTTTCAAAAAAAATCCTAAACCACCAACCTCATCAAAGATTTCATTTGATAACTATTTGATTTTTTTTTTTTTTTAAATTAAGCTTATAAATAATACTTCCACCTATGGGTTTCTTTGTTTTGTTATCTATTTTGATTTTATAAATATTAAAAAAAAAACCAATGAAACTGAAAAAAGTAGGTTTTTAAAAACTTGTTTTCATTAGACAGATCGAAATGATGGTAAACAAATTGTCTGAAAACCATCACAATTTTTAAAAACCAAGATCCTTTATAAAATCATTTGGGGTCGTTTGATAACCATTTTGTTTTTATTTTTTATTTTTTAAAACATGGTTGTTTGATAATCATTTCTGTTGTTTGTTTTTTTTTTTTTAAAAAACGTTTTTTAAATTATAAGCCAATTTCAAAAACAGATTTCTGTTTTTAAAAAACACATTTTTCTTCTAATGCTTTTTTTTTCCTCTTCTACCTATCTCTCTCCAACAAATTCTCTTTTTTTCTCTCCCTCTAAAACGTTTTTCTCTCTACAAATTTCTTTTTCTCTCTCAAAACTTTTTCTTCTACTTTTTTATTTCTAAAAATTTATCCATAACTTTTCTCTCCCTAGTAAACATATTCTAACTTTTCTTTTGAAAAAAAATCTCCCAGAATTTTCTCTCGCTAAGACTTTTCTTTATTTTTTTAATTTATCATTATTTCTTTGGCATATCATTTACTCTTAACCTTACAATCTCTCTAAATTTCTCCTTTTATTTAATTTTTCTATCTAAAATTTATCTCTTCTACTCTAACTTTTTTCTTTTTTCTCTAACTAATATCTTTTATGGAGTCTAACTATGTTTAAATTTTCTCTCGAAAAAATTTATATTTGTGTCTCTAAAGTTTTGTTTCAATGTCTCTACACATTTTTCTTTCTCTAAAACATCTTTTCATCTTTCTAATTTTTTAAAAATGAAAAACCAAAAACATTTATCAAACAAGTTTGGTTTTCAGTTTTTTAAAAACAGAAAACAAAAAACAGTTATCAAACATATATGGTTTTTGTTTTTAAAAAACATAAAAACCAAAAACCAAAAACTAAGGCCCCGTTTGATAACCATTTGGTTTTTGGTTTTTGGTTTTTCAAAATTATGCTTGTTTTCTCTCAATTTCCTTACCTTGGTTTTCATTTTGGTTAAGGAGACATTAGAGTTCTTAGCCAAATTCCAGAAACAAAAACAAGTTTTTTAAAACCACTTTTTTTTTGTTTTCAAAATTTGACTTGGTTTTTGAAAACATGGGAAGGATGTAAGTTCAAACCAAAGAAATTGATAGGTGGAAGAGTGTTTATAAGCTTAATTTTCAAAAACCAAAAACTAAAAACGAAATGGTTATCAAACAGGGTTATTTTTTGTTTTTAAAAATTAAGCTTATAAACACTCTACTCCCCTAAGTTTTTTTGTTTTGTTATTTACTTTCTTTCCATGTTTTCAAAAACTTGTTTTCGTTTTAGAATTTAGAAATAAAAATTATAAAAAAATAATATTGTAAAGAAGAGCCAAAATCTTTGGTCAAGTGACACTAATCTGTTTATTTGATCAGTAGCTTCCTTTTTTTTTTTTTTTTTTTGAGAAAATCAGTAGCTTTCCTTTTGGGGACACGTATCCAAATTCTTCAATGTCATTAGATTTCAGGTCAATTTCATGGAGATTTTCAAACAATAAACGCAACACATCTTCAACTTTCATTCATTTCTTAAAACCACAATTATCTTCGTCGAATCAATCAAAATCATCAATCAAATGGACAAGAACAACAACAAGAACAAGCACCTTCCATCAGCTTTAATTCTCGTGTGCCTCTTCCTCGCAGTTTCCACTTCCAAATCCAAAACCTCTCAGCCGCATTTTCACAAACACTTTGTGCTCATCCACGGCTCCTGCCATGGCGCCTGGTCCTGGTTCAAGCTCCTCCCTCTCCTCACATCCTCCGGCCACCGCGTCACCGCCCTCGACTTGGCCGCCTCCGGAATCGATCTCCGGCAGCCGGGCTCCGTCCGATCAATCTCCGAATACATCCGGCCTCTGATGGATTTCATGGTGGCTCTGCCTCAGCACCAGAAGGTGATCCTCGTGGGGCATAGCCTCGGCGGACTGGCCGTCGCCAAAGCCATGGAGGAGTTTCCGGCGAGGATTTCCGCCGCCGTTTTCGTCACTGCCTCGATGCCCGGGCCGGCTCTGAGCATATCGAGGATTTATCGGAAGGTGTTGGAAAGGAATGAATCGATGATGGATTGCGTTTACTCGTATGGCGATGGCCCCGATCGGCCTCCCACTGCCTTCGTCTTTGGGCCGCTCTATTTGGCTTCCAAAGTCTATCAGCTCAGCCCATCGGAGGTAAGGATTTGCAAAATTTTGAGTTTTTTTTTTTTTTTTTTTTTTCCTTTCTTCAAACTTTCGTAAAAGTAACAATTTACTTCATGAATTTTAGTTTGAAACAATTTAGTTCATGTACTTTTAATTTAGTAATGATTTAGTCTCTGAATTTTAATATATAACAATGTAGTCTGTGTATTTTAAGTTTTGTAACGGTTTTAGTCCTTGATGTGAAAATTTTCATCGAAATTAAGTGTCAATATTTATTATGTAACGACTTAGTCTTTGCCAAAGCAAGCTTAGCTTAGCGGTAATTGGCAACATTGCTAATGAGAGCAGTGCCGCTGTTTAGCGAAAAGGACATGTCCGATGAATTGAAGCTTTCAGAGGAGAAATACGGGTCGGTGAAACGGGTATTCGTCATGTCGGAAAAGGACTTGGTTGGAAACACACATTTTCAACGGTGGATGATTGAGAGAAACCCACCCGATCGTGTGGTGGAGATTGAAGGATCGGATCATATGGTCATGATGTCTAAGCCTTTGGAGCTGTGGGCCCATCTTCAACTCGTTGCCCAACACTACCATTAG

mRNA sequence

ATGCATTGGAAGTCATCAGATCTAGTGTTGTCCGAAGAGGCTGAGGACTTGTCAGCGTTGAAGTCCATCACCGACTCCATCGTTGGCCTCGCTTCTCGACTCCCTTCGGTCGAATTCTGCCACTGTAGTAGAGCCTCGAACTCAATCGCCCACTGCGTTGCGAGGCAAGCCTTTTCCACTTCCAAATCCAAAACCTCTCAGCCGCATTTTCACAAACACTTTGTGCTCATCCACGGCTCCTGCCATGGCGCCTGGTCCTGGTTCAAGCTCCTCCCTCTCCTCACATCCTCCGGCCACCGCGTCACCGCCCTCGACTTGGCCGCCTCCGGAATCGATCTCCGGCAGCCGGGCTCCGTCCGATCAATCTCCGAATACATCCGGCCTCTGATGGATTTCATGGTGGCTCTGCCTCAGCACCAGAAGGTGATCCTCGTGGGGCATAGCCTCGGCGGACTGGCCGTCGCCAAAGCCATGGAGGAGTTTCCGGCGAGGATTTCCGCCGCCGTTTTCGTCACTGCCTCGATGCCCGGGCCGGCTCTGAGCATATCGAGGATTTATCGGAAGGTGTTGGAAAGGAATGAATCGATGATGGATTGCGTTTACTCGTATGGCGATGGCCCCGATCGGCCTCCCACTGCCTTCGTCTTTGGGCCGCTCTATTTGGCTTCCAAAGTCTATCAGCTCAGCCCATCGGAGGACATGTCCGATGAATTGAAGCTTTCAGAGGAGAAATACGGGTCGGTGAAACGGGTATTCGTCATGTCGGAAAAGGACTTGGTTGGAAACACACATTTTCAACGGTGGATGATTGAGAGAAACCCACCCGATCGTGTGGTGGAGATTGAAGGATCGGATCATATGGTCATGATGTCTAAGCCTTTGGAGCTGTGGGCCCATCTTCAACTCGTTGCCCAACACTACCATTAG

Coding sequence (CDS)

ATGCATTGGAAGTCATCAGATCTAGTGTTGTCCGAAGAGGCTGAGGACTTGTCAGCGTTGAAGTCCATCACCGACTCCATCGTTGGCCTCGCTTCTCGACTCCCTTCGGTCGAATTCTGCCACTGTAGTAGAGCCTCGAACTCAATCGCCCACTGCGTTGCGAGGCAAGCCTTTTCCACTTCCAAATCCAAAACCTCTCAGCCGCATTTTCACAAACACTTTGTGCTCATCCACGGCTCCTGCCATGGCGCCTGGTCCTGGTTCAAGCTCCTCCCTCTCCTCACATCCTCCGGCCACCGCGTCACCGCCCTCGACTTGGCCGCCTCCGGAATCGATCTCCGGCAGCCGGGCTCCGTCCGATCAATCTCCGAATACATCCGGCCTCTGATGGATTTCATGGTGGCTCTGCCTCAGCACCAGAAGGTGATCCTCGTGGGGCATAGCCTCGGCGGACTGGCCGTCGCCAAAGCCATGGAGGAGTTTCCGGCGAGGATTTCCGCCGCCGTTTTCGTCACTGCCTCGATGCCCGGGCCGGCTCTGAGCATATCGAGGATTTATCGGAAGGTGTTGGAAAGGAATGAATCGATGATGGATTGCGTTTACTCGTATGGCGATGGCCCCGATCGGCCTCCCACTGCCTTCGTCTTTGGGCCGCTCTATTTGGCTTCCAAAGTCTATCAGCTCAGCCCATCGGAGGACATGTCCGATGAATTGAAGCTTTCAGAGGAGAAATACGGGTCGGTGAAACGGGTATTCGTCATGTCGGAAAAGGACTTGGTTGGAAACACACATTTTCAACGGTGGATGATTGAGAGAAACCCACCCGATCGTGTGGTGGAGATTGAAGGATCGGATCATATGGTCATGATGTCTAAGCCTTTGGAGCTGTGGGCCCATCTTCAACTCGTTGCCCAACACTACCATTAG

Protein sequence

MHWKSSDLVLSEEAEDLSALKSITDSIVGLASRLPSVEFCHCSRASNSIAHCVARQAFSTSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSKPLELWAHLQLVAQHYH
Homology
BLAST of Lcy05g012530 vs. ExPASy Swiss-Prot
Match: O80477 (Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.4e-55
Identity = 117/262 (44.66%), Postives = 162/262 (61.83%), Query Frame = 0

Query: 66  SQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDL-RQPGSVRSISE 125
           S+    +H VL+HG+CHGAW W+K+ P L +SGHRVTA+DLAASGID+ R    + +  +
Sbjct: 2   SEEERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQ 61

Query: 126 YIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISR 185
           Y  PLM  M +LP  +KV+LVGHSLGGL++A AM+ FP +IS +VFVTA MP    S S 
Sbjct: 62  YSEPLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSF 121

Query: 186 IYRKVLER--NESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSP------------ 245
           ++ K+ +    E  +D V++  + PD P   ++FGP ++A  +YQLSP            
Sbjct: 122 VWDKLRKETSREEWLDTVFT-SEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVR 181

Query: 246 -----SEDMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSD 305
                 +DM++    SEE YGSV R+F++  KDLV    +QR MI   PP  V+EI+ +D
Sbjct: 182 ANPLIKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDAD 241

Query: 306 HMVMMSKPLELWAHLQLVAQHY 308
           HM M SKP +L A L  +A  Y
Sbjct: 242 HMPMFSKPQQLCALLLEIANKY 262

BLAST of Lcy05g012530 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.4e-52
Identity = 111/256 (43.36%), Postives = 159/256 (62.11%), Query Frame = 0

Query: 72  KHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMD 131
           KHFVL+HG+CHG WSW+KL PLL ++GH+VTALDLAASG DLR+   +R++ +Y  PLM+
Sbjct: 5   KHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLME 64

Query: 132 FMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVLE 191
            M +L   +KVILVGHSLGG+ +  AME++P +I AAVF+ A MP    + S +  +  E
Sbjct: 65  LMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNE 124

Query: 192 RN--ESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQL-SPS----------------E 251
           R   E+ +D  +     P+ P T+  FGP +LA K+YQL SP                 E
Sbjct: 125 RTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLFME 184

Query: 252 DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSK 309
           D+S     ++E++GSVKRV+++  +D      FQRW I+       +EI+G+DHM M+ +
Sbjct: 185 DLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAMLCE 244

BLAST of Lcy05g012530 vs. ExPASy Swiss-Prot
Match: Q8S9K8 (Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.9e-52
Identity = 108/257 (42.02%), Postives = 153/257 (59.53%), Query Frame = 0

Query: 73  HFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMDF 132
           HFV +HGSCHGAW WFKL   L   GHRVTA+DL  SG+D RQ   VR +S Y+ PLM F
Sbjct: 21  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 80

Query: 133 MVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMP----GPALSISRIYRK 192
           M +LP+++KV+LVGHS GG+  + AME FP ++S  +F++A MP     PA+ I   + +
Sbjct: 81  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 140

Query: 193 VLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQ-------------LSPS---- 252
           + E     MDC +++ +G + PP++ +FG  +L  K Y              + PS    
Sbjct: 141 LPE--GFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYT 200

Query: 253 EDMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMS 309
           ++M  E  +++E+YGS KRVF++ E D V     Q+WMI    P  V  IE + HM M++
Sbjct: 201 KEMGGEDLITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLT 260

BLAST of Lcy05g012530 vs. ExPASy Swiss-Prot
Match: Q9LFT6 (Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.0e-49
Identity = 104/252 (41.27%), Postives = 153/252 (60.71%), Query Frame = 0

Query: 73  HFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMDF 132
           HFVL+H + HGAW W+KL PLL S+GHRVTA++LAASGID R   +V ++ EY +PL++ 
Sbjct: 6   HFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSKPLIET 65

Query: 133 MVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVLER 192
           + +LP++++VILVG S GG+ +A A + FPA+I   VF+ A +P      S +  K +E 
Sbjct: 66  LKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLDKYMEM 125

Query: 193 NESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSP-----------------SEDMS 252
              + DC +S  +  +   +    GP ++ +++YQ  P                 +ED+S
Sbjct: 126 PGGLGDCEFSSHETRNGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQGSFFTEDLS 185

Query: 253 DELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSKPLE 308
            + K SEE YGSV+RV+VMS +D      F RWMI+     +V EI+G DHMVM+SKP +
Sbjct: 186 KKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHMVMLSKPQK 245

BLAST of Lcy05g012530 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 8.7e-48
Identity = 96/244 (39.34%), Postives = 149/244 (61.07%), Query Frame = 0

Query: 72  KHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMD 131
           KHFVL+HG C GAW W+KL PLL S+GH+VTA+DL+A+GI+ R+   + +  +Y  PLM+
Sbjct: 11  KHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLME 70

Query: 132 FMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVLE 191
            M ++P  +KV+L+GHS GG+++  AME +P +IS AVF++A MP P  S++  + K  E
Sbjct: 71  VMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNE 130

Query: 192 R--NESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSE----------------- 251
           +   + M+D  +S    P+ P  + + GP ++A K++Q    E                 
Sbjct: 131 KCPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSLFFQ 190

Query: 252 DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSK 297
           D++   K S E+YGSVKR ++   +D      FQ+W +E    D+V EI+ +DHM M+S+
Sbjct: 191 DLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGMLSQ 250

BLAST of Lcy05g012530 vs. ExPASy TrEMBL
Match: A0A1S3BZM4 (methylesterase 3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495209 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 6.2e-105
Identity = 200/268 (74.63%), Postives = 214/268 (79.85%), Query Frame = 0

Query: 58  FSTSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPG 117
           F  S + +   HFHKHFV IHGSCHGAWSWFKLLPLL SSGHRVTALDLAASGID R P 
Sbjct: 23  FPLSDNTSHPHHFHKHFVFIHGSCHGAWSWFKLLPLLQSSGHRVTALDLAASGIDHRNPD 82

Query: 118 SVRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPG 177
           S+RSIS+Y +PL DFM ALPQHQKVILVGHSLGGLAVAKAMEEFP RISAAVFVTA+MPG
Sbjct: 83  SIRSISQYFQPLTDFMSALPQHQKVILVGHSLGGLAVAKAMEEFPTRISAAVFVTATMPG 142

Query: 178 PALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSE----- 237
           PAL+IS IY KV ERNESMMD VYSYGDG +RPPTAF+FGP +LASKVYQ SP E     
Sbjct: 143 PALNISTIYGKVFERNESMMDSVYSYGDGTNRPPTAFLFGPRFLASKVYQRSPPEDLTLA 202

Query: 238 -------------DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVV 297
                        DMSD LKLSE  YGSVKRVFVMS  DLV N  FQRWMIE N PDRVV
Sbjct: 203 TLLMRPVPLFTEKDMSDVLKLSERNYGSVKRVFVMSGMDLVSNKEFQRWMIENNSPDRVV 262

Query: 298 EIEGSDHMVMMSKPLELWAHLQLVAQHY 308
           E+EGSDHMVMMSKP +L AHLQL+AQ+Y
Sbjct: 263 EMEGSDHMVMMSKPFQLCAHLQLLAQYY 290

BLAST of Lcy05g012530 vs. ExPASy TrEMBL
Match: A0A0A0K4V3 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074920 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.6e-100
Identity = 191/253 (75.49%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 71  HKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLM 130
           HKHFV IHGSCHGAWSWFKLLPLL SSGHRVTALDLAASGID R P SVRSIS+Y +PL 
Sbjct: 31  HKHFVFIHGSCHGAWSWFKLLPLLQSSGHRVTALDLAASGIDHRNPDSVRSISQYFQPLT 90

Query: 131 DFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVL 190
           DFM ALPQHQKVILVGHSLGGL V+KAME+FP +ISAAVFVTA+MPGPAL+IS IY KV 
Sbjct: 91  DFMSALPQHQKVILVGHSLGGLVVSKAMEDFPTKISAAVFVTATMPGPALNISTIYSKVF 150

Query: 191 ERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSE------------------ 250
           ERNESMMD VYSYGDG +RPPTAF+FG  +LASKVYQ SP+E                  
Sbjct: 151 ERNESMMDSVYSYGDGRNRPPTAFLFGSRFLASKVYQRSPAEDLTLATLLMRAVPLFTEK 210

Query: 251 DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSK 306
           DMSD LKLSE  YGSVKRVFV+SE DLV N  FQRWMIE NPPD VVEIEGSDH+VMMSK
Sbjct: 211 DMSDVLKLSERNYGSVKRVFVVSEMDLVSNIEFQRWMIENNPPDHVVEIEGSDHVVMMSK 270

BLAST of Lcy05g012530 vs. ExPASy TrEMBL
Match: A0A2I4GLU0 (salicylic acid-binding protein 2-like OS=Juglans regia OX=51240 GN=LOC109008987 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 6.0e-84
Identity = 157/259 (60.62%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 67  QPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYI 126
           +P   KHFVL+HGSC GAWSW+KLLPLL SSGH VTALDLAASG++  QP  +RSIS + 
Sbjct: 32  RPSISKHFVLVHGSCLGAWSWYKLLPLLKSSGHNVTALDLAASGVNPVQPSDLRSISGFF 91

Query: 127 RPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIY 186
           RPL +FM  LP H++VILVGHSLGGLA+++AME FP+RIS AVF+TA MPGP L+IS + 
Sbjct: 92  RPLRNFMETLPPHERVILVGHSLGGLAISQAMESFPSRISVAVFLTALMPGPTLNISTLN 151

Query: 187 RKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDM------------ 246
           ++   R ++++D  +SY  GPD+PPT+F+FGP+YLAS V+QLSP+ED+            
Sbjct: 152 QESFSRQDTLLDSRFSYDKGPDKPPTSFIFGPMYLASNVFQLSPTEDLALATMLLRPLPL 211

Query: 247 ------SDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMV 306
                 S EL LS  KYGSV RV+++SEKD VG   FQ WMIERNPP+RVVEI GSDHMV
Sbjct: 212 FSDEDHSMELMLSTVKYGSVNRVYIISEKDKVGKRDFQWWMIERNPPNRVVEITGSDHMV 271

Query: 307 MMSKPLELWAHLQLVAQHY 308
           MMSKP +LWA LQ +A+ Y
Sbjct: 272 MMSKPKQLWARLQEIAEKY 290

BLAST of Lcy05g012530 vs. ExPASy TrEMBL
Match: A0A7J7HG73 (AB hydrolase-1 domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_009633 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 7.8e-84
Identity = 158/267 (59.18%), Postives = 198/267 (74.16%), Query Frame = 0

Query: 59  STSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGS 118
           +T  S+TS P   KHFVL+HGSCHGAWSW+KL+PLL SSGHRVTALDLAASGI+      
Sbjct: 21  ATIASETSPPTSSKHFVLVHGSCHGAWSWYKLVPLLRSSGHRVTALDLAASGINPELVDD 80

Query: 119 VRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGP 178
           + S+S+Y +PL DFM +LP+H++V+LVGHS GGLAV +AME FP +IS AVFVTA MPGP
Sbjct: 81  IPSVSDYFKPLQDFMASLPRHERVVLVGHSFGGLAVCQAMENFPEKISVAVFVTAMMPGP 140

Query: 179 ALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSED----- 238
           +L+IS ++++   R ES++D  Y+Y +GP+ P T F+FGPLYLA+KVYQLSP ED     
Sbjct: 141 SLNISTLFQESFRRQESVLDSHYTYDNGPNNPATTFIFGPLYLAAKVYQLSPIEDVALAT 200

Query: 239 -------------MSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVE 298
                        MS EL LS +KYGSV+RVFV+SEKD VG   FQ+W++E+NPPD V E
Sbjct: 201 TLLRPLRLYSLENMSRELVLSSKKYGSVRRVFVISEKDKVGKKEFQKWLVEKNPPDEVEE 260

Query: 299 IEGSDHMVMMSKPLELWAHLQLVAQHY 308
           I+GSDHMVMMSKP+EL   L  +A  Y
Sbjct: 261 IKGSDHMVMMSKPVELCVVLLGIAGKY 287

BLAST of Lcy05g012530 vs. ExPASy TrEMBL
Match: A0A5D3C473 (Methylesterase 3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00240 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 8.7e-83
Identity = 170/250 (68.00%), Postives = 183/250 (73.20%), Query Frame = 0

Query: 58  FSTSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPG 117
           F  S + +   HFHKHFV IHGSCHGAWSWFKLLPLL SSGHRVTALDLAASGID R P 
Sbjct: 23  FPLSDNTSHPHHFHKHFVFIHGSCHGAWSWFKLLPLLQSSGHRVTALDLAASGIDHRNPD 82

Query: 118 SVRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPG 177
           S+RSIS+Y +PL DFM ALPQHQKVILVGHSLGGLAVAKAMEEFP RISAAVFVTA+MPG
Sbjct: 83  SIRSISQYFQPLTDFMSALPQHQKVILVGHSLGGLAVAKAMEEFPTRISAAVFVTATMPG 142

Query: 178 PALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDMSDE 237
           PAL+IS IY K L     +M           RP   F                 +DMSD 
Sbjct: 143 PALNISTIYGKDLTLATLLM-----------RPVPLFT---------------EKDMSDV 202

Query: 238 LKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSKPLELW 297
           LKLSE  YGSVKRVFVMS  DLV N  FQRWMIE N PDRVVE+EGSDHMVMMSKP +L 
Sbjct: 203 LKLSERNYGSVKRVFVMSGMDLVSNKEFQRWMIENNSPDRVVEMEGSDHMVMMSKPFQLC 246

Query: 298 AHLQLVAQHY 308
           AHLQL+AQ+Y
Sbjct: 263 AHLQLLAQYY 246

BLAST of Lcy05g012530 vs. NCBI nr
Match: XP_008454904.1 (PREDICTED: methylesterase 3-like isoform X1 [Cucumis melo])

HSP 1 Score: 390.6 bits (1002), Expect = 1.3e-104
Identity = 200/268 (74.63%), Postives = 214/268 (79.85%), Query Frame = 0

Query: 58  FSTSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPG 117
           F  S + +   HFHKHFV IHGSCHGAWSWFKLLPLL SSGHRVTALDLAASGID R P 
Sbjct: 23  FPLSDNTSHPHHFHKHFVFIHGSCHGAWSWFKLLPLLQSSGHRVTALDLAASGIDHRNPD 82

Query: 118 SVRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPG 177
           S+RSIS+Y +PL DFM ALPQHQKVILVGHSLGGLAVAKAMEEFP RISAAVFVTA+MPG
Sbjct: 83  SIRSISQYFQPLTDFMSALPQHQKVILVGHSLGGLAVAKAMEEFPTRISAAVFVTATMPG 142

Query: 178 PALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSE----- 237
           PAL+IS IY KV ERNESMMD VYSYGDG +RPPTAF+FGP +LASKVYQ SP E     
Sbjct: 143 PALNISTIYGKVFERNESMMDSVYSYGDGTNRPPTAFLFGPRFLASKVYQRSPPEDLTLA 202

Query: 238 -------------DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVV 297
                        DMSD LKLSE  YGSVKRVFVMS  DLV N  FQRWMIE N PDRVV
Sbjct: 203 TLLMRPVPLFTEKDMSDVLKLSERNYGSVKRVFVMSGMDLVSNKEFQRWMIENNSPDRVV 262

Query: 298 EIEGSDHMVMMSKPLELWAHLQLVAQHY 308
           E+EGSDHMVMMSKP +L AHLQL+AQ+Y
Sbjct: 263 EMEGSDHMVMMSKPFQLCAHLQLLAQYY 290

BLAST of Lcy05g012530 vs. NCBI nr
Match: XP_004137122.1 (methylesterase 3 [Cucumis sativus] >KGN43959.1 hypothetical protein Csa_017298 [Cucumis sativus])

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-100
Identity = 191/253 (75.49%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 71  HKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLM 130
           HKHFV IHGSCHGAWSWFKLLPLL SSGHRVTALDLAASGID R P SVRSIS+Y +PL 
Sbjct: 31  HKHFVFIHGSCHGAWSWFKLLPLLQSSGHRVTALDLAASGIDHRNPDSVRSISQYFQPLT 90

Query: 131 DFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVL 190
           DFM ALPQHQKVILVGHSLGGL V+KAME+FP +ISAAVFVTA+MPGPAL+IS IY KV 
Sbjct: 91  DFMSALPQHQKVILVGHSLGGLVVSKAMEDFPTKISAAVFVTATMPGPALNISTIYSKVF 150

Query: 191 ERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSE------------------ 250
           ERNESMMD VYSYGDG +RPPTAF+FG  +LASKVYQ SP+E                  
Sbjct: 151 ERNESMMDSVYSYGDGRNRPPTAFLFGSRFLASKVYQRSPAEDLTLATLLMRAVPLFTEK 210

Query: 251 DMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSK 306
           DMSD LKLSE  YGSVKRVFV+SE DLV N  FQRWMIE NPPD VVEIEGSDH+VMMSK
Sbjct: 211 DMSDVLKLSERNYGSVKRVFVVSEMDLVSNIEFQRWMIENNPPDHVVEIEGSDHVVMMSK 270

BLAST of Lcy05g012530 vs. NCBI nr
Match: XP_018844854.1 (salicylic acid-binding protein 2-like [Juglans regia] >KAF5444014.1 hypothetical protein F2P56_036521 [Juglans regia])

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-83
Identity = 157/259 (60.62%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 67  QPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYI 126
           +P   KHFVL+HGSC GAWSW+KLLPLL SSGH VTALDLAASG++  QP  +RSIS + 
Sbjct: 32  RPSISKHFVLVHGSCLGAWSWYKLLPLLKSSGHNVTALDLAASGVNPVQPSDLRSISGFF 91

Query: 127 RPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIY 186
           RPL +FM  LP H++VILVGHSLGGLA+++AME FP+RIS AVF+TA MPGP L+IS + 
Sbjct: 92  RPLRNFMETLPPHERVILVGHSLGGLAISQAMESFPSRISVAVFLTALMPGPTLNISTLN 151

Query: 187 RKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDM------------ 246
           ++   R ++++D  +SY  GPD+PPT+F+FGP+YLAS V+QLSP+ED+            
Sbjct: 152 QESFSRQDTLLDSRFSYDKGPDKPPTSFIFGPMYLASNVFQLSPTEDLALATMLLRPLPL 211

Query: 247 ------SDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMV 306
                 S EL LS  KYGSV RV+++SEKD VG   FQ WMIERNPP+RVVEI GSDHMV
Sbjct: 212 FSDEDHSMELMLSTVKYGSVNRVYIISEKDKVGKRDFQWWMIERNPPNRVVEITGSDHMV 271

Query: 307 MMSKPLELWAHLQLVAQHY 308
           MMSKP +LWA LQ +A+ Y
Sbjct: 272 MMSKPKQLWARLQEIAEKY 290

BLAST of Lcy05g012530 vs. NCBI nr
Match: KAF5951689.1 (hypothetical protein HYC85_009633 [Camellia sinensis])

HSP 1 Score: 320.5 bits (820), Expect = 1.6e-83
Identity = 158/267 (59.18%), Postives = 198/267 (74.16%), Query Frame = 0

Query: 59  STSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGS 118
           +T  S+TS P   KHFVL+HGSCHGAWSW+KL+PLL SSGHRVTALDLAASGI+      
Sbjct: 21  ATIASETSPPTSSKHFVLVHGSCHGAWSWYKLVPLLRSSGHRVTALDLAASGINPELVDD 80

Query: 119 VRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGP 178
           + S+S+Y +PL DFM +LP+H++V+LVGHS GGLAV +AME FP +IS AVFVTA MPGP
Sbjct: 81  IPSVSDYFKPLQDFMASLPRHERVVLVGHSFGGLAVCQAMENFPEKISVAVFVTAMMPGP 140

Query: 179 ALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSED----- 238
           +L+IS ++++   R ES++D  Y+Y +GP+ P T F+FGPLYLA+KVYQLSP ED     
Sbjct: 141 SLNISTLFQESFRRQESVLDSHYTYDNGPNNPATTFIFGPLYLAAKVYQLSPIEDVALAT 200

Query: 239 -------------MSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVE 298
                        MS EL LS +KYGSV+RVFV+SEKD VG   FQ+W++E+NPPD V E
Sbjct: 201 TLLRPLRLYSLENMSRELVLSSKKYGSVRRVFVISEKDKVGKKEFQKWLVEKNPPDEVEE 260

Query: 299 IEGSDHMVMMSKPLELWAHLQLVAQHY 308
           I+GSDHMVMMSKP+EL   L  +A  Y
Sbjct: 261 IKGSDHMVMMSKPVELCVVLLGIAGKY 287

BLAST of Lcy05g012530 vs. NCBI nr
Match: XP_028102002.1 (polyneuridine-aldehyde esterase-like [Camellia sinensis])

HSP 1 Score: 320.1 bits (819), Expect = 2.1e-83
Identity = 157/267 (58.80%), Postives = 198/267 (74.16%), Query Frame = 0

Query: 59  STSKSKTSQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGS 118
           +T  S+TS P   KHFVL+HGSCHGAWSW+KL+PLL SSGHRVTALDLAASGI+      
Sbjct: 21  ATIASETSPPTSSKHFVLVHGSCHGAWSWYKLVPLLRSSGHRVTALDLAASGINPELVDD 80

Query: 119 VRSISEYIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGP 178
           + S+S+Y +PL DFM +LP+H++V+LVGHS GGLAV +AME FP +IS AVFVTA MPGP
Sbjct: 81  IPSVSDYFKPLQDFMASLPRHERVVLVGHSFGGLAVCQAMENFPEKISVAVFVTAMMPGP 140

Query: 179 ALSISRIYRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSED----- 238
           +L+IS ++++   R ES++D  Y+Y +GP+ P T F+FGPLYLA+KVYQLSP ED     
Sbjct: 141 SLNISTLFQESFRRQESVLDSHYTYDNGPNNPATTFIFGPLYLAAKVYQLSPIEDVALAT 200

Query: 239 -------------MSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVE 298
                        MS EL LS +KYGSV+RVFV+SEKD VG   FQ+W++E+NPPD V E
Sbjct: 201 TLLRPLRLYSLENMSRELVLSSKKYGSVRRVFVISEKDKVGKKEFQKWLVEKNPPDEVEE 260

Query: 299 IEGSDHMVMMSKPLELWAHLQLVAQHY 308
           I+GSDHM+MMSKP+EL   L  +A  Y
Sbjct: 261 IKGSDHMIMMSKPVELCVVLLGIAGKY 287

BLAST of Lcy05g012530 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 215.7 bits (548), Expect = 5.2e-56
Identity = 117/262 (44.66%), Postives = 162/262 (61.83%), Query Frame = 0

Query: 66  SQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDL-RQPGSVRSISE 125
           S+    +H VL+HG+CHGAW W+K+ P L +SGHRVTA+DLAASGID+ R    + +  +
Sbjct: 2   SEEERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQ 61

Query: 126 YIRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISR 185
           Y  PLM  M +LP  +KV+LVGHSLGGL++A AM+ FP +IS +VFVTA MP    S S 
Sbjct: 62  YSEPLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSF 121

Query: 186 IYRKVLER--NESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSP------------ 245
           ++ K+ +    E  +D V++  + PD P   ++FGP ++A  +YQLSP            
Sbjct: 122 VWDKLRKETSREEWLDTVFT-SEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVR 181

Query: 246 -----SEDMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSD 305
                 +DM++    SEE YGSV R+F++  KDLV    +QR MI   PP  V+EI+ +D
Sbjct: 182 ANPLIKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDAD 241

Query: 306 HMVMMSKPLELWAHLQLVAQHY 308
           HM M SKP +L A L  +A  Y
Sbjct: 242 HMPMFSKPQQLCALLLEIANKY 262

BLAST of Lcy05g012530 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 205.3 bits (521), Expect = 7.1e-53
Identity = 108/257 (42.02%), Postives = 153/257 (59.53%), Query Frame = 0

Query: 73  HFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMDF 132
           HFV +HGSCHGAW WFKL   L   GHRVTA+DL  SG+D RQ   VR +S Y+ PLM F
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 133 MVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMP----GPALSISRIYRK 192
           M +LP+++KV+LVGHS GG+  + AME FP ++S  +F++A MP     PA+ I   + +
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 153

Query: 193 VLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQ-------------LSPS---- 252
           + E     MDC +++ +G + PP++ +FG  +L  K Y              + PS    
Sbjct: 154 LPE--GFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYT 213

Query: 253 EDMSDELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMS 309
           ++M  E  +++E+YGS KRVF++ E D V     Q+WMI    P  V  IE + HM M++
Sbjct: 214 KEMGGEDLITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLT 273

BLAST of Lcy05g012530 vs. TAIR 10
Match: AT5G10300.1 (methyl esterase 5 )

HSP 1 Score: 196.1 bits (497), Expect = 4.3e-50
Identity = 104/252 (41.27%), Postives = 153/252 (60.71%), Query Frame = 0

Query: 73  HFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEYIRPLMDF 132
           HFVL+H + HGAW W+KL PLL S+GHRVTA++LAASGID R   +V ++ EY +PL++ 
Sbjct: 6   HFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSKPLIET 65

Query: 133 MVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKVLER 192
           + +LP++++VILVG S GG+ +A A + FPA+I   VF+ A +P      S +  K +E 
Sbjct: 66  LKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLDKYMEM 125

Query: 193 NESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSP-----------------SEDMS 252
              + DC +S  +  +   +    GP ++ +++YQ  P                 +ED+S
Sbjct: 126 PGGLGDCEFSSHETRNGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQGSFFTEDLS 185

Query: 253 DELKLSEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMMSKPLE 308
            + K SEE YGSV+RV+VMS +D      F RWMI+     +V EI+G DHMVM+SKP +
Sbjct: 186 KKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHMVMLSKPQK 245

BLAST of Lcy05g012530 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-47
Identity = 109/259 (42.08%), Postives = 150/259 (57.92%), Query Frame = 0

Query: 66  SQPHFHKHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGIDLRQPGSVRSISEY 125
           S+    +HFVL+HGSC GAW W+K+ PLL +SGHRVTALDLAA GID R    + +  +Y
Sbjct: 20  SEEMMKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQY 79

Query: 126 IRPLMDFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRI 185
             PL+  M +LP  +KV+LVGHS GGL +A AM++FP +IS +VFVT+ MP    S S  
Sbjct: 80  SEPLIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPS-- 139

Query: 186 YRKVLERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDMSDELKL----- 245
              VLE+  S M        G +  P   VF   +   ++ QLSP ED+   L L     
Sbjct: 140 --FVLEKFASTM--TPEDWMGSELEPYV-VFSAEFTKHRILQLSPIEDLELRLLLKRPGS 199

Query: 246 ------------SEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMV 305
                       SE+ YGSV R +++S+ D   +  +QRWMI+  PP+ V+E+EG+DH+ 
Sbjct: 200 LFLNDLSRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLP 259

Query: 306 MMSKPLELWAHLQLVAQHY 308
           +  KP  L  HL  +A  +
Sbjct: 260 LFCKPQLLSDHLLAIADKF 271

BLAST of Lcy05g012530 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 186.8 bits (473), Expect = 2.6e-47
Identity = 107/257 (41.63%), Postives = 150/257 (58.37%), Query Frame = 0

Query: 72  KHFVLIHGSCHGAWSWFKLLPLLTSSGHRVTALDLAASGID-LRQPGSVRSISEYIRPLM 131
           +HFVL+HG+CHGAW W+K+ PLL + GHRVTALDLAASGID  R    + +  +Y  PLM
Sbjct: 8   QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67

Query: 132 DFMVALPQHQKVILVGHSLGGLAVAKAMEEFPARISAAVFVTASMPGPALSISRIYRKV- 191
             M +LP  +KV+LVGHS GGL++A AM++FP +IS +VFVTA MP    S S +  K  
Sbjct: 68  QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127

Query: 192 --LERNESMMDCVYSYGDGPDRPPTAFVFGPLYLASKVYQLSPSEDMSDELKL------- 251
             +     M   + +YG   D    +  F   ++  ++YQLSP ED+   L L       
Sbjct: 128 SSMTPEGWMGSELETYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLF 187

Query: 252 ----------SEEKYGSVKRVFVMSEKDLVGNTHFQRWMIERNPPDRVVEIEGSDHMVMM 308
                     SE+ YGSV R +++ ++D + +   QRWMI   P + V+E+E +DHM M 
Sbjct: 188 INELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPMF 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O804777.4e-5544.66Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1[more]
Q6RYA03.4e-5243.36Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1[more]
Q8S9K89.9e-5242.02Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1[more]
Q9LFT66.0e-4941.27Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1[more]
Q9SE938.7e-4839.34Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3BZM46.2e-10574.63methylesterase 3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495209 PE=4 SV... [more]
A0A0A0K4V31.6e-10075.49AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074... [more]
A0A2I4GLU06.0e-8460.62salicylic acid-binding protein 2-like OS=Juglans regia OX=51240 GN=LOC109008987 ... [more]
A0A7J7HG737.8e-8459.18AB hydrolase-1 domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_0... [more]
A0A5D3C4738.7e-8368.00Methylesterase 3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
XP_008454904.11.3e-10474.63PREDICTED: methylesterase 3-like isoform X1 [Cucumis melo][more]
XP_004137122.13.2e-10075.49methylesterase 3 [Cucumis sativus] >KGN43959.1 hypothetical protein Csa_017298 [... [more]
XP_018844854.11.2e-8360.62salicylic acid-binding protein 2-like [Juglans regia] >KAF5444014.1 hypothetical... [more]
KAF5951689.11.6e-8359.18hypothetical protein HYC85_009633 [Camellia sinensis][more]
XP_028102002.12.1e-8358.80polyneuridine-aldehyde esterase-like [Camellia sinensis][more]
Match NameE-valueIdentityDescription
AT2G23610.15.2e-5644.66methyl esterase 3 [more]
AT3G50440.17.1e-5342.02methyl esterase 10 [more]
AT5G10300.14.3e-5041.27methyl esterase 5 [more]
AT2G23590.11.5e-4742.08methyl esterase 8 [more]
AT2G23600.12.6e-4741.63acetone-cyanohydrin lyase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 69..308
e-value: 4.5E-64
score: 218.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 72..300
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 74..298
e-value: 2.0E-18
score: 67.8
NoneNo IPR availablePANTHERPTHR10992:SF1002ESTERASE PIR7A-RELATEDcoord: 55..306
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 55..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g012530.1Lcy05g012530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity