Lcy05g012270 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g012270
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptioncalmodulin-like protein 1
LocationChr05: 14175196 .. 14176285 (+)
RNA-Seq ExpressionLcy05g012270
SyntenyLcy05g012270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCACTTTGCTTCGCACACCACCACAAGTTTTCAACGAATCAAAATTCATCTTCTCCTCCTCCATTTCCACCATTTAAGCCCTTCAAATCCTTCAGGGTTCGTCTTAAATTTCAAAACCAATCTCCTCTGTTTCAGCGACAAAAAAAAAAAAACAGAGCACTCAAATCAATCTTCCTCTGTTTCAACGACAAAAAACAGAGCAACCCAGTTCTCATTACTTCAACGCAATCTTCCTCTGTTTCAGCGAAAAACAGAGCAACCCAACAGACAAAAGGGGGCTGAGAAGATGCCGATGAATCCGAATCTGCTGGACCTGCAATACAACGTCTCGAAGAAAAAGTTTCTCCGGGAGCCCTCGCGGCTGTTCTCGTTCAAGGACCGGCAGGGCTCCGGGCTGCGGCGGGCGCTGCAGCCAAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGGATCTCGAAGCACGAGTACAGGGGAATTCTGAAGGCGCTGGGGAGAGGGAACTCCATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGACGCGGACGGAGATGGGTATATAAACTTGAACGAGTTCATGGAGGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGAGAGGTGGAATTTGCTTTCAAGACATTCGATTTGAATGGGGATCGGAAGATTAGTGCGGAGGAGGTGATGAGAGTGTTGAGGGGATTGGGGGAAAAGTGTAGCATCGAGGATTGCCGGAGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACCATGATGACTCGCTCTGGAAAGCATGTTTAGGAGAGCTCTTTCTTTAATGGAGTTTGGTGGATTGTGCAAGTGTTTTATGTAAATTAAGTGCTCTGTTAATTTGCAATTATAGCCACTTCATAATCATACTTTCAGTTTAAGAACTAAAATGTTTGTGAATGTGGACTGTGTTCGTAGATTTATAGATGAGCTAAAGAATCGATTAATATTTCCGATATATGATGAAATATGAAGAAAAAAAAAATCATCCTAGTTCAATCAAACATATATCA

mRNA sequence

TCCACTTTGCTTCGCACACCACCACAAGTTTTCAACGAATCAAAATTCATCTTCTCCTCCTCCATTTCCACCATTTAAGCCCTTCAAATCCTTCAGGGTTCGTCTTAAATTTCAAAACCAATCTCCTCTGTTTCAGCGACAAAAAAAAAAAAACAGAGCACTCAAATCAATCTTCCTCTGTTTCAACGACAAAAAACAGAGCAACCCAGTTCTCATTACTTCAACGCAATCTTCCTCTGTTTCAGCGAAAAACAGAGCAACCCAACAGACAAAAGGGGGCTGAGAAGATGCCGATGAATCCGAATCTGCTGGACCTGCAATACAACGTCTCGAAGAAAAAGTTTCTCCGGGAGCCCTCGCGGCTGTTCTCGTTCAAGGACCGGCAGGGCTCCGGGCTGCGGCGGGCGCTGCAGCCAAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGGATCTCGAAGCACGAGTACAGGGGAATTCTGAAGGCGCTGGGGAGAGGGAACTCCATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGACGCGGACGGAGATGGGTATATAAACTTGAACGAGTTCATGGAGGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGAGAGGTGGAATTTGCTTTCAAGACATTCGATTTGAATGGGGATCGGAAGATTAGTGCGGAGGAGGTGATGAGAGTGTTGAGGGGATTGGGGGAAAAGTGTAGCATCGAGGATTGCCGGAGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACCATGATGACTCGCTCTGGAAAGCATGTTTAGGAGAGCTCTTTCTTTAATGGAGTTTGGTGGATTGTGCAAGTGTTTTATGTAAATTAAGTGCTCTGTTAATTTGCAATTATAGCCACTTCATAATCATACTTTCAGTTTAAGAACTAAAATGTTTGTGAATGTGGACTGTGTTCGTAGATTTATAGATGAGCTAAAGAATCGATTAATATTTCCGATATATGATGAAATATGAAGAAAAAAAAAATCATCCTAGTTCAATCAAACATATATCA

Coding sequence (CDS)

ATGCCGATGAATCCGAATCTGCTGGACCTGCAATACAACGTCTCGAAGAAAAAGTTTCTCCGGGAGCCCTCGCGGCTGTTCTCGTTCAAGGACCGGCAGGGCTCCGGGCTGCGGCGGGCGCTGCAGCCAAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGAGGATCTCGAAGCACGAGTACAGGGGAATTCTGAAGGCGCTGGGGAGAGGGAACTCCATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGACGCGGACGGAGATGGGTATATAAACTTGAACGAGTTCATGGAGGTTCACCGGAGTGGCGGCGGAGTTCAGGCGAGAGAGGTGGAATTTGCTTTCAAGACATTCGATTTGAATGGGGATCGGAAGATTAGTGCGGAGGAGGTGATGAGAGTGTTGAGGGGATTGGGGGAAAAGTGTAGCATCGAGGATTGCCGGAGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGCATGGTGGACATTGATGAGTTCATGACCATGATGACTCGCTCTGGAAAGCATGTTTAG

Protein sequence

MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSGKHV
Homology
BLAST of Lcy05g012270 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-50
Identity = 97/182 (53.30%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY +SK K LR+PSR+FS +DRQ SGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARE 123
           +DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

BLAST of Lcy05g012270 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-28
Identity = 59/141 (41.84%), Postives = 92/141 (65.25%), Query Frame = 0

Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFM 105
           EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++    DADGDG+I+L+EF 
Sbjct: 39  EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFA 98

Query: 106 EVHRSGGGVQA---REVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAV 165
            ++ +  G  A    ++  AF+ FD +G+  ISA E+ RVL GLGEK +++ CRRM+  V
Sbjct: 99  ALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGV 158

Query: 166 DSDGDGMVDIDEFMTMMTRSG 184
           D +GDG++  +EF  MM   G
Sbjct: 159 DQNGDGLISFEEFKVMMAGGG 179

BLAST of Lcy05g012270 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-28
Identity = 63/155 (40.65%), Postives = 103/155 (66.45%), Query Frame = 0

Query: 35  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 94
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFM---EVHRSGGGVQAREV---EFAFKTFDLNGDRKISAEEVMRVLRGLGE 154
           G+G+I+L+EF+   ++   GGG    +V   + AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSG 184
           KCS++DC++M+  VD DGDG V+ DEF  MM+  G
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNGG 159

BLAST of Lcy05g012270 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.0e-28
Identity = 64/152 (42.11%), Postives = 93/152 (61.18%), Query Frame = 0

Query: 37  LRRALQPSTE--EIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 96
           L++A    TE  E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  
Sbjct: 25  LQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRK 84

Query: 97  GDGYINLNEFMEVHRSGGGVQ--AREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSI 156
           GDGYIN  EF+E++  G         ++ AF  +D++G+  ISAEE+  VLR LG++CSI
Sbjct: 85  GDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSI 144

Query: 157 EDCRRMVRAVDSDGDGMVDIDEFMTMMTRSGK 185
            +CR+M+  VD DGDG +D +EF  MMT   +
Sbjct: 145 AECRKMIGGVDKDGDGTIDFEEFKIMMTMGSR 176

BLAST of Lcy05g012270 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.9e-28
Identity = 62/144 (43.06%), Postives = 95/144 (65.97%), Query Frame = 0

Query: 44  STEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNE 103
           S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEV----HRSGGGVQAREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMV 163
           F+ +     +S      R+++ AF  +DL+ + +ISA E+  V++ LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDSDGDGMVDIDEFMTMMTRSG 184
             VDSDGDG VD +EF  MM  +G
Sbjct: 132 NKVDSDGDGCVDFEEFKKMMMING 155

BLAST of Lcy05g012270 vs. ExPASy TrEMBL
Match: A0A6J1JEH1 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 8.6e-94
Identity = 175/182 (96.15%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQY VSKKKFLREPSRLFSFKDRQ SG+RRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD DGDGYINLNEFMEVHRSG GVQAREVE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

BLAST of Lcy05g012270 vs. ExPASy TrEMBL
Match: A0A0A0K804 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.3e-93
Identity = 173/184 (94.02%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA+EVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSGK 185
           RS K
Sbjct: 181 RSAK 184

BLAST of Lcy05g012270 vs. ExPASy TrEMBL
Match: A0A5D3C643 (Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00450 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.5e-93
Identity = 173/184 (94.02%), Postives = 180/184 (97.83%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA+EVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSGK 185
           RS K
Sbjct: 181 RSAK 184

BLAST of Lcy05g012270 vs. ExPASy TrEMBL
Match: A0A1S3C0X9 (calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.5e-93
Identity = 173/184 (94.02%), Postives = 180/184 (97.83%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA+EVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSGK 185
           RS K
Sbjct: 181 RSAK 184

BLAST of Lcy05g012270 vs. ExPASy TrEMBL
Match: A0A6J1ELL0 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-92
Identity = 173/182 (95.05%), Postives = 177/182 (97.25%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQY VSKKKFLREPSRLFSFKDRQ SG+RRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD DGDGYINLNEFMEVHRSG GVQAR+VE
Sbjct: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

BLAST of Lcy05g012270 vs. NCBI nr
Match: XP_022988967.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 352.8 bits (904), Expect = 1.8e-93
Identity = 175/182 (96.15%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQY VSKKKFLREPSRLFSFKDRQ SG+RRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD DGDGYINLNEFMEVHRSG GVQAREVE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQAREVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

BLAST of Lcy05g012270 vs. NCBI nr
Match: KAG6588656.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013282.1 Calmodulin-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 351.7 bits (901), Expect = 4.0e-93
Identity = 174/182 (95.60%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQY VSKKKFLREPSRLFSFKDRQ SG+RRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRG+LKALGRGNSMEEVQKIFRAVD DGDGYINLNEFMEVHRSG GVQAR+VE
Sbjct: 61  GRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

BLAST of Lcy05g012270 vs. NCBI nr
Match: XP_004137114.2 (calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Csa_017094 [Cucumis sativus])

HSP 1 Score: 350.5 bits (898), Expect = 8.8e-93
Identity = 173/184 (94.02%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA+EVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSGK 185
           RS K
Sbjct: 181 RSAK 184

BLAST of Lcy05g012270 vs. NCBI nr
Match: XP_038888181.1 (calmodulin-like protein 30 [Benincasa hispida])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 174/185 (94.05%), Postives = 180/185 (97.30%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPNLLDLQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPSTEEIKQIFNKFD NRD
Sbjct: 1   MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSTEEIKQIFNKFDRNRD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISK+EYRGILKALGR NSMEEVQ IFRAVDADGDGYINLNEFMEVHRSGGGVQARE+E
Sbjct: 61  GRISKNEYRGILKALGRENSMEEVQNIFRAVDADGDGYINLNEFMEVHRSGGGVQAREIE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVD+DGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDNDGDGMVDINEFMTMMT 180

Query: 181 RSGKH 186
           RS K+
Sbjct: 181 RSAKY 185

BLAST of Lcy05g012270 vs. NCBI nr
Match: XP_008454944.1 (PREDICTED: calmodulin-like protein 1 [Cucumis melo] >KAA0031292.1 calmodulin-like protein 1 [Cucumis melo var. makuwa] >TYK06744.1 calmodulin-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 173/184 (94.02%), Postives = 180/184 (97.83%), Query Frame = 0

Query: 1   MPMNPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQYNVSKKKFLREPSRLFSFKDRQ SGLRRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQAREVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA+EVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSGK 185
           RS K
Sbjct: 181 RSAK 184

BLAST of Lcy05g012270 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 201.1 bits (510), Expect = 8.0e-52
Identity = 97/182 (53.30%), Postives = 142/182 (78.02%), Query Frame = 0

Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQGSGLRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY +SK K LR+PSR+FS +DRQ SGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARE 123
           +DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

BLAST of Lcy05g012270 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 125.9 bits (315), Expect = 3.3e-29
Identity = 63/155 (40.65%), Postives = 103/155 (66.45%), Query Frame = 0

Query: 35  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 94
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFM---EVHRSGGGVQAREV---EFAFKTFDLNGDRKISAEEVMRVLRGLGE 154
           G+G+I+L+EF+   ++   GGG    +V   + AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSG 184
           KCS++DC++M+  VD DGDG V+ DEF  MM+  G
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNGG 159

BLAST of Lcy05g012270 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 125.6 bits (314), Expect = 4.3e-29
Identity = 64/152 (42.11%), Postives = 93/152 (61.18%), Query Frame = 0

Query: 37  LRRALQPSTE--EIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 96
           L++A    TE  E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  
Sbjct: 25  LQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRK 84

Query: 97  GDGYINLNEFMEVHRSGGGVQ--AREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSI 156
           GDGYIN  EF+E++  G         ++ AF  +D++G+  ISAEE+  VLR LG++CSI
Sbjct: 85  GDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSI 144

Query: 157 EDCRRMVRAVDSDGDGMVDIDEFMTMMTRSGK 185
            +CR+M+  VD DGDG +D +EF  MMT   +
Sbjct: 145 AECRKMIGGVDKDGDGTIDFEEFKIMMTMGSR 176

BLAST of Lcy05g012270 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 125.2 bits (313), Expect = 5.6e-29
Identity = 62/144 (43.06%), Postives = 95/144 (65.97%), Query Frame = 0

Query: 44  STEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNE 103
           S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEV----HRSGGGVQAREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMV 163
           F+ +     +S      R+++ AF  +DL+ + +ISA E+  V++ LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDSDGDGMVDIDEFMTMMTRSG 184
             VDSDGDG VD +EF  MM  +G
Sbjct: 132 NKVDSDGDGCVDFEEFKKMMMING 155

BLAST of Lcy05g012270 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-28
Identity = 63/137 (45.99%), Postives = 89/137 (64.96%), Query Frame = 0

Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAREVEFAFKTFDLNGDRKISAEEVMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQE61.1e-5053.30Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1[more]
Q8RZB54.6e-2841.84Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P250704.6e-2840.65Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2[more]
Q9FYK26.0e-2842.11Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q9C8Y17.9e-2843.06Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JEH18.6e-9496.15calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111486169 PE=4 SV=1[more]
A0A0A0K8044.3e-9394.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1[more]
A0A5D3C6439.5e-9394.02Calmodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3C0X99.5e-9394.02calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1[more]
A0A6J1ELL01.2e-9295.05calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111434472 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_022988967.11.8e-9396.15calmodulin-like protein 1 [Cucurbita maxima][more]
KAG6588656.14.0e-9395.60Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_004137114.28.8e-9394.02calmodulin-like protein 30 [Cucumis sativus] >KGN43936.1 hypothetical protein Cs... [more]
XP_038888181.12.0e-9294.05calmodulin-like protein 30 [Benincasa hispida][more]
XP_008454944.12.0e-9294.02PREDICTED: calmodulin-like protein 1 [Cucumis melo] >KAA0031292.1 calmodulin-lik... [more]
Match NameE-valueIdentityDescription
AT2G15680.18.0e-5253.30Calcium-binding EF-hand family protein [more]
AT5G37770.13.3e-2940.65EF hand calcium-binding protein family [more]
AT1G24620.14.3e-2942.11EF hand calcium-binding protein family [more]
AT1G66400.15.6e-2943.06calmodulin like 23 [more]
AT1G18210.12.1e-2845.99Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 83..111
e-value: 3.7E-5
score: 33.1
coord: 154..182
e-value: 0.0063
score: 25.7
coord: 47..75
e-value: 7.2E-7
score: 38.8
coord: 118..146
e-value: 4.6E-4
score: 29.5
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 119..179
e-value: 2.9E-12
score: 46.8
coord: 46..106
e-value: 2.0E-14
score: 53.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 150..185
score: 14.262346
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 43..78
score: 15.685013
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 79..114
score: 14.5413
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 118..149
score: 12.504936
IPR002048EF-hand domainCDDcd00051EFhcoord: 118..180
e-value: 3.27073E-18
score: 72.5805
IPR002048EF-hand domainCDDcd00051EFhcoord: 47..109
e-value: 9.33013E-19
score: 74.1213
NoneNo IPR availableGENE3D1.10.238.10coord: 6..111
e-value: 1.5E-23
score: 85.7
NoneNo IPR availableGENE3D1.10.238.10coord: 114..185
e-value: 5.4E-21
score: 76.4
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 8..184
NoneNo IPR availablePANTHERPTHR10891:SF685CALCIUM-BINDING PROTEIN CML34-RELATEDcoord: 8..184
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 92..104
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 163..175
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 127..139
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 56..68
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 45..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g012270.1Lcy05g012270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005509 calcium ion binding