Lcy05g011800 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g011800
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	rRNA N-glycosidase
Location	Chr05: 13475534 .. 13476604 (+)
RNA-Seq Expression	Lcy05g011800
Synteny	Lcy05g011800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACCACACGTTGAGATGCTCCAAAGGCATCCAAAAATATCGAGTGGGAAAGATGAAGAGATTTACAGTGCTAATTCTCGCCATCTTCCTTGCAGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAAACAATAAACAAAATGTTGCAGCTTTTGACGAGGATGTTTCTGCAAAACACTGAGAGCCTCGGAGTCTGTTGTGTGTATTTGGAGTTTAATGCTCTTGTGAAAATTAATAAAAGCATGTTCATGTGATCTAACTACGTGAGTGCTCTGTATCTCTGTTTTGTTTCTTTAAATGAGTAATTAAGATTATATCTACTAGTTGAATGGAGTAGGCTTCCAAAGAAGATTGATC

mRNA sequence

AAAACCACACGTTGAGATGCTCCAAAGGCATCCAAAAATATCGAGTGGGAAAGATGAAGAGATTTACAGTGCTAATTCTCGCCATCTTCCTTGCAGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAAACAATAAACAAAATGTTGCAGCTTTTGACGAGGATGTTTCTGCAAAACACTGAGAGCCTCGGAGTCTGTTGTGTGTATTTGGAGTTTAATGCTCTTGTGAAAATTAATAAAAGCATGTTCATGTGATCTAACTACGTGAGTGCTCTGTATCTCTGTTTTGTTTCTTTAAATGAGTAATTAAGATTATATCTACTAGTTGAATGGAGTAGGCTTCCAAAGAAGATTGATC

Coding sequence (CDS)

ATGAAGAGATTTACAGTGCTAATTCTCGCCATCTTCCTTGCAGCTTCAACTGTTGAAGCCGATGTGAGCTTCAGTTTGTCAGGTTCTTCCTCCACATCTTATAGCAAGTTCATCGGAGATCTGAGGAAGGCTCTTCCATCTAGTGGGACAGTCTACAACATAACTCTCTTACTCTCCTCTGCCTCGGGCGCAAGCCGCTACACTCTCATGAAACTCTCCAATTACGACGGCAAAGCCATCACGGTTGCCATAGATGTAACCAACGTTTACATCATGGGCTATCTCGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAATTAGCTTCTCAATACGTATTCAAAGGCAGTACCATCGTCACGCTTCCATATTCTGGCAATTACGAAAAGCTTCAAACTGCTGAAGGGAAGATAAGAGAGAAGATTCCACTGGGATTCCCAGCTTTGGACAGTGCGATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTCCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTATATCGAGGGACAGATTATTGAGAGAATTTCGAAAAACGGGGTGCCGAGTCTGGCGACTATAAGTTTAGAAAACGAATGGTCTGCTCTCTCTAAACAAATTCAGTTGGAACAGACGAATAACGGAACGTTTAAAACTCCCGTTGTGATAACGGACGATAAAGGCCAACGAGTTGAAATAACCAACGTCACTTCGAAAGTTGTAACCAAGAACATCAAATTGCTTCTAAACAAGTAA

Protein sequence

MKRFTVLILAIFLAASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLLNK

Homology

[Close]

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 7.0e-131
Identity = 252/261 (96.55%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MKRFTVLILAIFLAASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSS 60
           MKRFTVLILAIF+AASTVEADV FSLSGSSSTSYSKFIGDLRKALPS+GTVYNITLLLSS
Sbjct: 1   MKRFTVLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSS 60

Query: 61  ASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST 120
           ASGASRYTLM LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST
Sbjct: 61  ASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST 120

Query: 121 IVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA 180
           IVTLPYSGNYEKLQTA GKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA
Sbjct: 121 IVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA 180

Query: 181 SRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQ 240
           SRFKYIEGQIIERISKN VPSLATISLENEWSALSKQIQL QTNNGTFKTPVVITDDKGQ
Sbjct: 181 SRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQ 240

Query: 241 RVEITNVTSKVVTKNIKLLLN 262
           RVEITNVTSKVVTKNI+LLLN
Sbjct: 241 RVEITNVTSKVVTKNIQLLLN 261

[Close]

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 422.5 bits (1085), Expect = 3.4e-117
Identity = 228/241 (94.61%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 21  DVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 80
           DVSFSLSGSSSTSYSKFIG LRKALPS+GTVYNITLLLSSASGASRYTLMKLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 81  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKI 140
           TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYEKLQTA GKI
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 141 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVP 200
           REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE+SRFKYIEGQII RISKNGVP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 201 SLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLL 260
           SLATISLENEWSALSKQIQL QTNNGTFKTPVVI D  GQRVEI NV SKVVTKNI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 261 N 262
           N
Sbjct: 241 N 241

[Close]

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 7.1e-99
Identity = 200/246 (81.30%), Postives = 215/246 (87.40%), Query Frame = 0


Query: 20  ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKA 79
           A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNYD KA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 80  ITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGK 139
           IT+AIDVTNVYIMGYLVNSTSYF NESDAKLASQYVFKGST+VT+PYSGNYE+LQ A GK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGK 120

Query: 140 IREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGV 199
           IREKIPLGF ALDSA+T++FHYDSTAAAAAFLVI+QTTAEASRFKYIEGQIIERI KN V
Sbjct: 121 IREKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEV 180

Query: 200 PSLATISLENE-WSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTK--NI 259
           PS A +SLENE WS LSKQIQL QTNNG F+TPVVI D+KGQRVEITN+ SKV  K  N 
Sbjct: 181 PSPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNS 240

Query: 260 KLLLNK 263
           KLLLNK
Sbjct: 241 KLLLNK 246

[Close]

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.2e-91
Identity = 187/265 (70.57%), Postives = 210/265 (79.25%), Query Frame = 0


Query: 1   MKRFTV---LILAIFLAASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLL 60
           M RF+V   LILAIFL  S V+ DVSF LSG+   SY  FI DLR ALP    VYNI LL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLL 60

Query: 61  LSSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFK 120
           L S SGA RY LM L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LASQYVF+
Sbjct: 61  LPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFR 120

Query: 121 GS-TIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQT 180
            +   +TLPYSGNYE+LQ A GK REKIP+G PALDSAI+TL HYDSTAAA A LV+IQT
Sbjct: 121 DARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQT 180

Query: 181 TAEASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITD 240
           TAEA+RFKYIE QI ER  ++ VPSLATISLEN WS LSKQIQL Q NNG F+TP+V+ D
Sbjct: 181 TAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVD 240

Query: 241 DKGQRVEITNVTSKVVTKNIKLLLN 262
           +KG RV+ITNVTSKVVT NI+LLLN
Sbjct: 241 NKGNRVQITNVTSKVVTSNIQLLLN 265

[Close]

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-86
Identity = 174/268 (64.93%), Postives = 214/268 (79.85%), Query Frame = 0


Query: 1   MKRFTVL----ILAIFLAASTVEADVS--FSLSGSSSTSYSKFIGDLRKALPSSGTVYNI 60
           M RF+VL    IL+IF    T E DV+  FSL GS+  SYSKFI  +R ALP++G +YNI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  TLLLSSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQY 120
            LL+ S SG+ RY LM+LSNY+G  IT+A+DVTNVYIMGYLVN TSYFFNE+DA+LAS++
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSTIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVII 180
           VF+G+  +TLPYSGNY+KLQ+   K R+ IPLGF ALDSAI+TL++YDS +A  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVI 240
           QTTAEA+R+KYIE QII+RIS + VP LA ISLENEWS LSKQIQ+ ++NNG F+TPV I
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 TDDKGQRVEITNVTSKVVTKNIKLLLNK 263
            +DKG   E+TNV+S VVTKNI LLLNK
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNK 268

[Close]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.7e-103
Identity = 216/264 (81.82%), Postives = 229/264 (86.74%), Query Frame = 0


Query: 1   MKRFTVL-ILAIFLAASTVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLL 60
           M RFT L +L I +A  TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  SSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
            SASGASRY LM+LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTA 180
           STIVTLPYSGNYE+LQ A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDK 240
           EASRFKYIEGQIIERI KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+K
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEITNVTSKVVTKNIKLLLNK 263
           GQRVEI +V SKVVT NIKLLLNK
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNK 264

[Close]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.7e-103
Identity = 216/264 (81.82%), Postives = 229/264 (86.74%), Query Frame = 0


Query: 1   MKRFTVL-ILAIFLAASTVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLL 60
           M RFT L +L I +A  TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  SSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
            SASGASRY LM+LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTA 180
           STIVTLPYSGNYE+LQ A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDK 240
           EASRFKYIEGQIIERI KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+K
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEITNVTSKVVTKNIKLLLNK 263
           GQRVEI +V SKVVT NIKLLLNK
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNK 264

[Close]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 6.6e-100
Identity = 203/259 (78.38%), Postives = 227/259 (87.64%), Query Frame = 0


Query: 4   FTVLILAIFLAAST-VEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSAS 63
           F ++ILAIF  +S+ V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 17  FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 76

Query: 64  GASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123
           GASRYT +KLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 77  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 136

Query: 124 TLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183
           TLPYSGNYEKLQ A GK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 137 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 196

Query: 184 FKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRV 243
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRV
Sbjct: 197 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 256

Query: 244 EITNVTSKVVTKNIKLLLN 262
           EITNV+SKVV KNI+LLLN
Sbjct: 257 EITNVSSKVVAKNIQLLLN 275

[Close]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 8.7e-100
Identity = 204/259 (78.76%), Postives = 226/259 (87.26%), Query Frame = 0


Query: 4   FTVLILAIFLAAST-VEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSAS 63
           F ++ILAIF  +S+ V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 17  FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSAT 76

Query: 64  GASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123
           GASRYT +KLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 77  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 136

Query: 124 TLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183
           TLPYSGNYEKLQ A GK REKIPLGFPALDSAITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 137 TLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASR 196

Query: 184 FKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRV 243
           +KYIE Q+I+RISK+ VPS A ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRV
Sbjct: 197 YKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 256

Query: 244 EITNVTSKVVTKNIKLLLN 262
           EITNV+S VVTKNI+LLLN
Sbjct: 257 EITNVSSNVVTKNIQLLLN 275

[Close]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.2e-88
Identity = 187/265 (70.57%), Postives = 210/265 (79.25%), Query Frame = 0


Query: 1   MKRFTV---LILAIFLAASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLL 60
           M RF+V   LILAIFL  S V+ DVSF LSG+   SY  FI DLR ALP    VYNI LL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLL 60

Query: 61  LSSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFK 120
           L S SGA RY LM L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LASQYVF+
Sbjct: 61  LPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFR 120

Query: 121 GS-TIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQT 180
            +   +TLPYSGNYE+LQ A GK REKIP+G PALDSAI+TL HYDSTAAA A LV+IQT
Sbjct: 121 DARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQT 180

Query: 181 TAEASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITD 240
           TAEA+RFKYIE QI ER  ++ VPSLATISLEN WS LSKQIQL Q NNG F+TP+V+ D
Sbjct: 181 TAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVD 240

Query: 241 DKGQRVEITNVTSKVVTKNIKLLLN 262
           +KG RV+ITNVTSKVVT NI+LLLN
Sbjct: 241 NKGNRVQITNVTSKVVTSNIQLLLN 265

[Close]

BLAST of Lcy05g011800 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 468.0 bits (1203), Expect = 5.3e-128
Identity = 252/261 (96.55%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MKRFTVLILAIFLAASTVEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSS 60
           MKRFTVLILAIF+AASTVEADV FSLSGSSSTSYSKFIGDLRKALPS+GTVYNITLLLSS
Sbjct: 1   MKRFTVLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSS 60

Query: 61  ASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST 120
           ASGASRYTLM LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST
Sbjct: 61  ASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGST 120

Query: 121 IVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA 180
           IVTLPYSGNYEKLQTA GKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA
Sbjct: 121 IVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEA 180

Query: 181 SRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQ 240
           SRFKYIEGQIIERISKN VPSLATISLENEWSALSKQIQL QTNNGTFKTPVVITDDKGQ
Sbjct: 181 SRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQ 240

Query: 241 RVEITNVTSKVVTKNIKLLLN 262
           RVEITNVTSKVVTKNI+LLLN
Sbjct: 241 RVEITNVTSKVVTKNIQLLLN 261

[Close]

BLAST of Lcy05g011800 vs. NCBI nr
Match: P84530.2 (RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-glycosidase [Luffa acutangula] >2OQA_A X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula] >2OQA_B X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula])

HSP 1 Score: 422.5 bits (1085), Expect = 2.6e-114
Identity = 228/241 (94.61%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 21  DVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 80
           DVSFSLSGSSSTSYSKFIG LRKALPS+GTVYNITLLLSSASGASRYTLMKLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 81  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGKI 140
           TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYEKLQTA GKI
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 141 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGVP 200
           REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE+SRFKYIEGQII RISKNGVP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 201 SLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLLL 260
           SLATISLENEWSALSKQIQL QTNNGTFKTPVVI D  GQRVEI NV SKVVTKNI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 261 N 262
           N
Sbjct: 241 N 241

[Close]

BLAST of Lcy05g011800 vs. NCBI nr
Match: ABY71834.1 (ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aegyptiaca])

HSP 1 Score: 385.6 bits (989), Expect = 3.5e-103
Identity = 216/264 (81.82%), Postives = 229/264 (86.74%), Query Frame = 0


Query: 1   MKRFTVL-ILAIFLAASTVE-ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLL 60
           M RFT L +L I +A  TVE A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  SSASGASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
            SASGASRY LM+LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTA 180
           STIVTLPYSGNYE+LQ A GK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDK 240
           EASRFKYIEGQIIERI KN VPS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+K
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEITNVTSKVVTKNIKLLLNK 263
           GQRVEI +V SKVVT NIKLLLNK
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNK 264

[Close]

BLAST of Lcy05g011800 vs. NCBI nr
Match: 1NIO_A (Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution [Luffa aegyptiaca])

HSP 1 Score: 377.1 bits (967), Expect = 1.2e-100
Identity = 204/243 (83.95%), Postives = 216/243 (88.89%), Query Frame = 0


Query: 20  ADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSASGASRYTLMKLSNYDGKA 79
           A+VSFSLSG+ S SYSKFI  LRKALPS   V NI LLL SASGASRY LM+LSNYD KA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 80  ITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAEGK 139
           IT+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYE+LQ A GK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 120

Query: 140 IREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNGV 199
           +REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAEASRFKYIEGQII+RI KN V
Sbjct: 121 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEV 180

Query: 200 PSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIKLL 259
           PS A +SLENEWSALSKQIQL QTNNG F+TPVVI D+KGQRVEI +V SKVVT NIKLL
Sbjct: 181 PSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLL 240

Query: 260 LNK 263
           LNK
Sbjct: 241 LNK 243

[Close]

BLAST of Lcy05g011800 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 373.6 bits (958), Expect = 1.4e-99
Identity = 203/259 (78.38%), Postives = 227/259 (87.64%), Query Frame = 0


Query: 4   FTVLILAIFLAAST-VEADVSFSLSGSSSTSYSKFIGDLRKALPSSGTVYNITLLLSSAS 63
           F ++ILAIF  +S+ V  DVSF+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 17  FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 76

Query: 64  GASRYTLMKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123
           GASRYT +KLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 77  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 136

Query: 124 TLPYSGNYEKLQTAEGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183
           TLPYSGNYEKLQ A GK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 137 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 196

Query: 184 FKYIEGQIIERISKNGVPSLATISLENEWSALSKQIQLEQTNNGTFKTPVVITDDKGQRV 243
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQL QTN G FK PV+ITDD+GQRV
Sbjct: 197 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 256

Query: 244 EITNVTSKVVTKNIKLLLN 262
           EITNV+SKVV KNI+LLLN
Sbjct: 257 EITNVSSKVVAKNIQLLLN 275

The following BLAST results are available for this feature:

BLAST of Lcy05g011800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	7.0e-131	96.55	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	3.4e-117	94.61	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P22851	7.1e-99	81.30	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	3.2e-91	70.57	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q9FRX4	3.1e-86	64.93	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of Lcy05g011800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B0EVM6	1.7e-103	81.82	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	1.7e-103	81.82	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1GMM2	6.6e-100	78.38	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]
A0A6J1JVG7	8.7e-100	78.76	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1DSN8	1.2e-88	70.57	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1	[more]

BLAST of Lcy05g011800 vs. NCBI nr


Match Name	E-value	Identity	Description
Q00465.1	5.3e-128	96.55	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]
P84530.2	2.6e-114	94.61	RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-g...	[more]
ABY71834.1	3.5e-103	81.82	ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aeg...	[more]
1NIO_A	1.2e-100	83.95	Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolu...	[more]
XP_022953306.1	1.4e-99	78.38	ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata]	[more]

BLAST of Lcy05g011800 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 79..94 score: 52.3 coord: 202..219 score: 52.34 coord: 141..155 score: 56.84 coord: 170..190 score: 58.15 coord: 32..45 score: 45.3
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 20..181 e-value: 1.4E-44 score: 153.5
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 182..262 e-value: 1.6E-27 score: 97.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 30..219 e-value: 7.3E-30 score: 104.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 21..258
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 21..258
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 174..190
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 21..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g011800.1	Lcy05g011800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity