Lcy05g011770 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g011770
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	rRNA N-glycosidase
Location	Chr05: 13441953 .. 13442789 (+)
RNA-Seq Expression	Lcy05g011770
Synteny	Lcy05g011770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAGATTCACATTTCTCTCTTTGCTAATAATTCTCATTGCTTTCTTTACTGTTGAAGGTGCTAATGTGAGCTTCAGTTTGTCGGGGGCTGATTCCAAATCCTACAGCAAGTTCATCACAGCTCTGAGGAAGGCTCTTCCATCTAAGGAGAAAGTGTCCAATATACCTCTATTGCTTCCATCCGCTTCCGGCGCAAGTCGCTACATACTAATGCAACTCTCCAATTACGACGCCAAAGCCATCACAATGGCTATAGATGTAACAAACGTTTACATTATGGGCTATCTTGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAACTAGCTTCTCAATACGTATTCAAAGGTAGTACCATCGTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAAATGCTGCAGGCAAAGTGAGAGAAAAAATTCCACTTGGATTCCGAGCTTTCGACAGTGCAATTACCTCCTTGTTTCATTACGACTCCACGGCTGCTGCTGGGGCATTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTACATTGAGGGACAGATTATTGAGAGAATTCCCAAAAACGAGGTGCCGAGTCCAGCGGCTCTAAGTTTAGAAAACGAATGGTCTGCTCTCTCCAAACAGATTCAATTAGCACAAACTAACAACGGAGCGTTTAGAACTCCCGTTGTGATTATAGACAATAAAGGCCAACGAGTTGAAATAAAGGACGTTAATTCAAAAGTTGTAACCAACAACATCAAGTTGCTTCTAAACAAACAAAATATTGCAGCTTTTGACGATGGTATTCCTACAAAACACTGA

mRNA sequence

ATGAACAGATTCACATTTCTCTCTTTGCTAATAATTCTCATTGCTTTCTTTACTGTTGAAGGTGCTAATGTGAGCTTCAGTTTGTCGGGGGCTGATTCCAAATCCTACAGCAAGTTCATCACAGCTCTGAGGAAGGCTCTTCCATCTAAGGAGAAAGTGTCCAATATACCTCTATTGCTTCCATCCGCTTCCGGCGCAAGTCGCTACATACTAATGCAACTCTCCAATTACGACGCCAAAGCCATCACAATGGCTATAGATGTAACAAACGTTTACATTATGGGCTATCTTGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAACTAGCTTCTCAATACGTATTCAAAGGTAGTACCATCGTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAAATGCTGCAGGCAAAGTGAGAGAAAAAATTCCACTTGGATTCCGAGCTTTCGACAGTGCAATTACCTCCTTGTTTCATTACGACTCCACGGCTGCTGCTGGGGCATTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTACATTGAGGGACAGATTATTGAGAGAATTCCCAAAAACGAGGTGCCGAGTCCAGCGGCTCTAAGTTTAGAAAACGAATGGTCTGCTCTCTCCAAACAGATTCAATTAGCACAAACTAACAACGGAGCGTTTAGAACTCCCGTTGTGATTATAGACAATAAAGGCCAACGAGTTGAAATAAAGGACGTTAATTCAAAAGTTGTAACCAACAACATCAAGTTGCTTCTAAACAAACAAAATATTGCAGCTTTTGACGATGGTATTCCTACAAAACACTGA

Coding sequence (CDS)

ATGAACAGATTCACATTTCTCTCTTTGCTAATAATTCTCATTGCTTTCTTTACTGTTGAAGGTGCTAATGTGAGCTTCAGTTTGTCGGGGGCTGATTCCAAATCCTACAGCAAGTTCATCACAGCTCTGAGGAAGGCTCTTCCATCTAAGGAGAAAGTGTCCAATATACCTCTATTGCTTCCATCCGCTTCCGGCGCAAGTCGCTACATACTAATGCAACTCTCCAATTACGACGCCAAAGCCATCACAATGGCTATAGATGTAACAAACGTTTACATTATGGGCTATCTTGTCAATTCAACATCCTACTTTTTCAACGAGTCTGATGCTAAACTAGCTTCTCAATACGTATTCAAAGGTAGTACCATCGTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAAATGCTGCAGGCAAAGTGAGAGAAAAAATTCCACTTGGATTCCGAGCTTTCGACAGTGCAATTACCTCCTTGTTTCATTACGACTCCACGGCTGCTGCTGGGGCATTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCCAGATTCAAGTACATTGAGGGACAGATTATTGAGAGAATTCCCAAAAACGAGGTGCCGAGTCCAGCGGCTCTAAGTTTAGAAAACGAATGGTCTGCTCTCTCCAAACAGATTCAATTAGCACAAACTAACAACGGAGCGTTTAGAACTCCCGTTGTGATTATAGACAATAAAGGCCAACGAGTTGAAATAAAGGACGTTAATTCAAAAGTTGTAACCAACAACATCAAGTTGCTTCTAAACAAACAAAATATTGCAGCTTTTGACGATGGTATTCCTACAAAACACTGA

Protein sequence

MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH

Homology

[Close]

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 6.5e-119
Identity = 229/250 (91.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 22  ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 81
           ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 82  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 141
           ITMAIDVTNVYIMGYLVNSTSYF NESDAKLASQYVFKGST+VT+PYSGNYERLQNAAGK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGK 120

Query: 142 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEV 201
           +REKIPLGFRA DSA+TS+FHYDSTAAA AFLVI+QTTAEASRFKYIEGQIIERIPKNEV
Sbjct: 121 IREKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEV 180

Query: 202 PSPAALSLENE-WSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTN--NI 261
           PSPAALSLENE WS LSKQIQLAQTNNGAFRTPVVIIDNKGQRVEI ++ SKV     N 
Sbjct: 181 PSPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNS 240

Query: 262 KLLLNKQNIA 269
           KLLLNKQNIA
Sbjct: 241 KLLLNKQNIA 250

[Close]

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.6e-112
Identity = 223/279 (79.93%), Postives = 241/279 (86.38%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60
           M RFT L +L I +A  TVE A+V FSLSG+ S SYSKFI  LRKALPS   V NI LLL
Sbjct: 1   MKRFTVL-ILAIFVAASTVE-ADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLL 60

Query: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
            SASGASRY LM LSNYD KAIT+A+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  SSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180
           STIVTLPYSGNYE+LQ AAGK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240
           EASRFKYIEGQIIERI KN+VPS A +SLENEWSALSKQIQLAQTNNG F+TPVVI D+K
Sbjct: 181 EASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDK 240

Query: 241 GQRVEIKDVNSKVVTNNIKLLLN-KQNIAAFDDGIPTKH 279
           GQRVEI +V SKVVT NI+LLLN KQN+AAFD+ +  KH
Sbjct: 241 GQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDEDVSAKH 277

[Close]

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 5.0e-103
Identity = 201/241 (83.40%), Postives = 214/241 (88.80%), Query Frame = 0


Query: 23  NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 82
           +VSFSLSG+ S SYSKFI ALRKALPS   V NI LLL SASGASRY LM+LSNYD KAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 83  TMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKV 142
           T+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYE+LQ AAGK+
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 143 REKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEVP 202
           REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAE+SRFKYIEGQII RI KN VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 203 SPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLL 262
           S A +SLENEWSALSKQIQLAQTNNG F+TPVVI+D  GQRVEI +V SKVVT NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 263 N 264
           N
Sbjct: 241 N 241

[Close]

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 5.6e-94
Identity = 188/281 (66.90%), Postives = 223/281 (79.36%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAF-FTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLL 60
           M+RF+ LS LI+ I    ++   +VSF LSGAD +SY  FI  LR ALP +EKV NIPLL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLL 60

Query: 61  LPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFK 120
           LPS SGA RY+LM L NYD K IT+A+DVTNVYIMGYL ++TSYFFNE  A+LASQYVF+
Sbjct: 61  LPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFR 120

Query: 121 GS-TIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180
            +   +TLPYSGNYERLQ AAGK REKIP+G  A DSAI++L HYDSTAAAGA LV+IQT
Sbjct: 121 DARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQT 180

Query: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240
           TAEA+RFKYIE QI ER  ++EVPS A +SLEN WS LSKQIQLAQ NNG FRTP+V++D
Sbjct: 181 TAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVD 240

Query: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDG-IPTKH 279
           NKG RV+I +V SKVVT+NI+LLLN +NIA  D+G + T H
Sbjct: 241 NKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 281

[Close]

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.9e-90
Identity = 176/278 (63.31%), Postives = 223/278 (80.22%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFF---TVEG-ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNI 60
           MNRF+ L  L+IL  F    T EG   V FSL G++ KSYSKFIT++R ALP+   + NI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PLLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQY 120
           PLL+PS SG+ RYILMQLSNY+   ITMA+DVTNVYIMGYLVN TSYFFNE+DA+LAS++
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVII 180
           VF+G+  +TLPYSGNY++LQ+ A K R+ IPLGF A DSAI++L++YDS +A  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVI 240
           QTTAEA+R+KYIE QII+RI  ++VP  AA+SLENEWS LSKQIQ+A++NNG F+TPV I
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 IDNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGI 275
           I++KG   E+ +V+S VVT NI LLLNK NIA+F+D +
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHV 278

[Close]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.7e-146
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60
           MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
           PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180
           STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240
           EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 279
           GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.7e-146
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60
           MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
           PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180
           STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240
           EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 279
           GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 9.5e-97
Identity = 192/263 (73.00%), Postives = 223/263 (84.79%), Query Frame = 0


Query: 7   LSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGA 66
           L +L I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GA
Sbjct: 19  LVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGA 78

Query: 67  SRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTL 126
           SRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TL
Sbjct: 79  SRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITL 138

Query: 127 PYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFK 186
           PYSGNYE+LQNAAGK REKIPLGF A DSAIT+LFHYDSTAAA AF+V+IQ TAEASR+K
Sbjct: 139 PYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYK 198

Query: 187 YIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEI 246
           YIE Q+I+RI K++VPSPA +SLEN WSALSKQIQLAQTN G F+ PV+I D++GQRVEI
Sbjct: 199 YIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEI 258

Query: 247 KDVNSKVVTNNIKLLLNKQNIAA 270
            +V+S VVT NI+LLLN QNIAA
Sbjct: 259 TNVSSNVVTKNIQLLLNSQNIAA 281

[Close]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 7.5e-94
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 0


Query: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 269
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

[Close]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL
Match: A0A6J1GMJ7 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.6e-91
Identity = 183/270 (67.78%), Postives = 224/270 (82.96%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAF--FTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60
           MNR + LSLLI+ I     T  G N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPL
Sbjct: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60

Query: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120
           LLP+  G++RY LM+LSNY+ K IT+AIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF
Sbjct: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120

Query: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180
           +G+  + LPYSGNY++LQ AA K R+ IPLGF A D+AI++L+HYD+ AA  AFLV+IQT
Sbjct: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180

Query: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240
           TAEASRFKYIE QII+RI +NEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +I+
Sbjct: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240

Query: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 269
           +KG  VE+ +V+S VVTNNIKLLLNKQN+A
Sbjct: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 270

[Close]

BLAST of Lcy05g011770 vs. NCBI nr
Match: ABY71834.1 (ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aegyptiaca])

HSP 1 Score: 528.5 bits (1360), Expect = 3.5e-146
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60
           MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL
Sbjct: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60

Query: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
           PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180
           STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240
           EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK
Sbjct: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240

Query: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 279
           GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH
Sbjct: 241 GQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Lcy05g011770 vs. NCBI nr
Match: 1NIO_A (Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution [Luffa aegyptiaca])

HSP 1 Score: 468.0 bits (1203), Expect = 5.7e-128
Identity = 246/247 (99.60%), Postives = 247/247 (100.00%), Query Frame = 0


Query: 22  ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 81
           ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 82  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 141
           ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 120

Query: 142 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEV 201
           VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQII+RIPKNEV
Sbjct: 121 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEV 180

Query: 202 PSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLL 261
           PSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLL
Sbjct: 181 PSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLL 240

Query: 262 LNKQNIA 269
           LNKQNIA
Sbjct: 241 LNKQNIA 247

[Close]

BLAST of Lcy05g011770 vs. NCBI nr
Match: P22851.1 (RecName: Full=Ribosome-inactivating protein luffin-B; AltName: Full=rRNA N-glycosidase [Luffa aegyptiaca])

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-116
Identity = 229/250 (91.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 22  ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 81
           ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA
Sbjct: 1   ANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKA 60

Query: 82  ITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGK 141
           ITMAIDVTNVYIMGYLVNSTSYF NESDAKLASQYVFKGST+VT+PYSGNYERLQNAAGK
Sbjct: 61  ITMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGK 120

Query: 142 VREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEV 201
           +REKIPLGFRA DSA+TS+FHYDSTAAA AFLVI+QTTAEASRFKYIEGQIIERIPKNEV
Sbjct: 121 IREKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEV 180

Query: 202 PSPAALSLENE-WSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTN--NI 261
           PSPAALSLENE WS LSKQIQLAQTNNGAFRTPVVIIDNKGQRVEI ++ SKV     N 
Sbjct: 181 PSPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNS 240

Query: 262 KLLLNKQNIA 269
           KLLLNKQNIA
Sbjct: 241 KLLLNKQNIA 250

[Close]

BLAST of Lcy05g011770 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 407.1 bits (1045), Expect = 1.2e-109
Identity = 223/279 (79.93%), Postives = 241/279 (86.38%), Query Frame = 0


Query: 1   MNRFTFLSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLL 60
           M RFT L +L I +A  TVE A+V FSLSG+ S SYSKFI  LRKALPS   V NI LLL
Sbjct: 1   MKRFTVL-ILAIFVAASTVE-ADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLL 60

Query: 61  PSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120
            SASGASRY LM LSNYD KAIT+A+DVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG
Sbjct: 61  SSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKG 120

Query: 121 STIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTA 180
           STIVTLPYSGNYE+LQ AAGK+REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTA
Sbjct: 121 STIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTA 180

Query: 181 EASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNK 240
           EASRFKYIEGQIIERI KN+VPS A +SLENEWSALSKQIQLAQTNNG F+TPVVI D+K
Sbjct: 181 EASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDK 240

Query: 241 GQRVEIKDVNSKVVTNNIKLLLN-KQNIAAFDDGIPTKH 279
           GQRVEI +V SKVVT NI+LLLN KQN+AAFD+ +  KH
Sbjct: 241 GQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDEDVSAKH 277

[Close]

BLAST of Lcy05g011770 vs. NCBI nr
Match: P84530.2 (RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-glycosidase [Luffa acutangula] >2OQA_A X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula] >2OQA_B X-ray Sequence and Crystal Structure of Luffaculin 1, a Novel Type 1 Ribosome-inactivating Protein [Luffa acutangula])

HSP 1 Score: 375.6 bits (963), Expect = 3.8e-100
Identity = 201/241 (83.40%), Postives = 214/241 (88.80%), Query Frame = 0


Query: 23  NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 82
           +VSFSLSG+ S SYSKFI ALRKALPS   V NI LLL SASGASRY LM+LSNYD KAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 83  TMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKV 142
           T+AIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF GSTIVTLPYSGNYE+LQ AAGK+
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 143 REKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIERIPKNEVP 202
           REKIPLGF A DSAIT+LFHYDSTAAA AFLVIIQTTAE+SRFKYIEGQII RI KN VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 203 SPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLL 262
           S A +SLENEWSALSKQIQLAQTNNG F+TPVVI+D  GQRVEI +V SKVVT NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 263 N 264
           N
Sbjct: 241 N 241

The following BLAST results are available for this feature:

BLAST of Lcy05g011770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22851	6.5e-119	91.60	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q00465	1.6e-112	79.93	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	5.0e-103	83.40	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P16094	5.6e-94	66.90	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q9FRX4	2.9e-90	63.31	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of Lcy05g011770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B0EVM6	1.7e-146	100.00	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	1.7e-146	100.00	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1JVG7	9.5e-97	73.00	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1GMM2	7.5e-94	70.00	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]
A0A6J1GMJ7	1.6e-91	67.78	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1	[more]

BLAST of Lcy05g011770 vs. NCBI nr


Match Name	E-value	Identity	Description
ABY71834.1	3.5e-146	100.00	ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aeg...	[more]
1NIO_A	5.7e-128	99.60	Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolu...	[more]
P22851.1	5.0e-116	91.60	RecName: Full=Ribosome-inactivating protein luffin-B; AltName: Full=rRNA N-glyco...	[more]
Q00465.1	1.2e-109	79.93	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]
P84530.2	3.8e-100	83.40	RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-g...	[more]

BLAST of Lcy05g011770 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 172..192 score: 58.15 coord: 204..221 score: 51.17 coord: 81..96 score: 50.99 coord: 34..47 score: 47.18 coord: 143..157 score: 49.47
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 32..221 e-value: 3.9E-33 score: 114.7
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 23..266
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..268 e-value: 1.1E-28 score: 100.9
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 22..183 e-value: 8.6E-47 score: 160.8
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 23..266
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g011770.1	Lcy05g011770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity