Lcy05g009660 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g009660
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	pectinesterase inhibitor-like
Location	Chr05: 10071309 .. 10071949 (-)
RNA-Seq Expression	Lcy05g009660
Synteny	Lcy05g009660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAAATGGCGAATTCCATCATCCTTTCAGCCTATTCTCTTCTAGCTTTCTTCCTTTCACTTGTTCTGTCCACGCATGCGGCTTCCTCAAACGACGTCGTCTCCACCATCTGCTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACGGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACAGTCCCTAGCGTCGAAGGCCGCCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGTGCCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGAACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACGGAGGCCGACGACTGTCAGGACAACTTCGCGCAGCCGCCGACGGACCCATCGGCGCTGTCCGCCAACGCTAAGGCTCTGGAGGATATTTGTAGTATTATCTTGGTCATAGCCAATCTTCTCCTTGGCCGCATTTGATAAAAAAAATGATTGCATCTCTCTGGCTCTCATCTATTCTGATCTGATAACTATACATTGTAATCTTCTTTCATAATTCAATAAACTTTTTTCTACTTGGAA

mRNA sequence

CCAAAATGGCGAATTCCATCATCCTTTCAGCCTATTCTCTTCTAGCTTTCTTCCTTTCACTTGTTCTGTCCACGCATGCGGCTTCCTCAAACGACGTCGTCTCCACCATCTGCTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACGGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACAGTCCCTAGCGTCGAAGGCCGCCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGTGCCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGAACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACGGAGGCCGACGACTGTCAGGACAACTTCGCGCAGCCGCCGACGGACCCATCGGCGCTGTCCGCCAACGCTAAGGCTCTGGAGGATATTTGTAGTATTATCTTGGTCATAGCCAATCTTCTCCTTGGCCGCATTTGATAAAAAAAATGATTGCATCTCTCTGGCTCTCATCTATTCTGATCTGATAACTATACATTGTAATCTTCTTTCATAATTCAATAAACTTTTTTCTACTTGGAA

Coding sequence (CDS)

ATGGCGAATTCCATCATCCTTTCAGCCTATTCTCTTCTAGCTTTCTTCCTTTCACTTGTTCTGTCCACGCATGCGGCTTCCTCAAACGACGTCGTCTCCACCATCTGCTCAAGAACTAGAAACCCTCCCTTTTGCACCAACGTCTTGAAATCTGCTGGCACCACGGAGCTGAAAGGGCTGGCCACCTTCACCCTGAACCTGGCCCGCACGAGGGCTGGCGAGAGCCGCGTCCTGGCACAGTCCCTAGCGTCGAAGGCCGCCGATCCCAAGCTCAAGGAGCGCTACGCCTCTTGTGCCGAAAACTACGACGATGCCGTTGGCGACATCGACGATGCCGGGAAGAACTTGGCGTCGGGTGACTACAATGGCGTCAACGTTCACGCTTCTGGAGCCATGACGGAGGCCGACGACTGTCAGGACAACTTCGCGCAGCCGCCGACGGACCCATCGGCGCTGTCCGCCAACGCTAAGGCTCTGGAGGATATTTGTAGTATTATCTTGGTCATAGCCAATCTTCTCCTTGGCCGCATTTGA

Protein sequence

MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGRI

Homology

[Close]

BLAST of Lcy05g009660 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-26
Identity = 69/177 (38.98%), Postives = 107/177 (60.45%), Query Frame = 0


Query: 2   ANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKS---AGTTELK 61
           ++S+ +S   ++ F   L       + N ++S IC +TRNP  C   L+S   + + +LK
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 66

Query: 62  GLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLAS 121
           GL  F++++A+  A ++  +  SL ++A DPKLK RY +C+ENY DA+  +  A + L S
Sbjct: 67  GLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTS 126

Query: 122 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 176
           GDYN +N++AS A   A  C+D+F  PP  P+ L      LED+C I+LVI+NLL G
Sbjct: 127 GDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Lcy05g009660 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-18
Identity = 61/174 (35.06%), Postives = 106/174 (60.92%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSA-GTTELKG 60
           MA ++  +A+     FL L+ S++A +S++ +STIC +T NP FC   L +   +  L+ 
Sbjct: 1   MAANLRNNAFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQA 60

Query: 61  LATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLASG 120
           LA  TL+  + RA ++    QS+     DP+ K  Y SC + Y+ A+G++++A ++LASG
Sbjct: 61  LAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASG 120

Query: 121 DYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 174
           D  G+N+  S A+  AD C D+  +  +  S++  N+K ++++C I LVI+N+L
Sbjct: 121 DGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Lcy05g009660 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.0e-18
Identity = 61/176 (34.66%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTT---EL 60
           + N +++S  SL+ F ++  L+   A     +  IC + +N  FCT+ +KS   T   +L
Sbjct: 5   LTNRVLMS--SLMFFVMTGSLNAQVAD----IKAICGKAKNQSFCTSYMKSNPKTSGADL 64

Query: 61  KGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLA 120
           + LA  T   A+T A E     QSL   A +P +K+ Y SC ++Y  A+  ++DA ++LA
Sbjct: 65  QTLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLA 124

Query: 121 SGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 174
           SGD  G+N+  S AM     C+ + A    DPSA+  N+   ++IC I+LVI+N++
Sbjct: 125 SGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVK-NSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL
Match: A0A6J1I1S1 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111469059 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.7e-55
Identity = 125/177 (70.62%), Postives = 140/177 (79.10%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGL 60
           MA+S  L A SL+   + L+L  HAAS NDV STIC +TRNP FC NVLKSAGTT+LKGL
Sbjct: 1   MASSSFL-AVSLIVLSV-LLLPMHAASQNDVASTICKKTRNPSFCFNVLKSAGTTDLKGL 60

Query: 61  ATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLASGD 120
           ATFTLNLA  +  E+R LAQSL SKAADPKLKERYA+CAE YD+A GDI+D   NL  GD
Sbjct: 61  ATFTLNLAHDKVAETRALAQSLTSKAADPKLKERYATCAELYDNAAGDIEDGKVNLGEGD 120

Query: 121 YNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGRI 178
           YNGVN+ A  AMTEA DC D+FAQPP DPSALS N K +EDICSIILVIANLLLGR+
Sbjct: 121 YNGVNIKAFAAMTEAGDCLDSFAQPPKDPSALSGNGKTVEDICSIILVIANLLLGRV 175

[Close]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL
Match: A0A6J1F073 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111437853 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 7.5e-55
Identity = 123/178 (69.10%), Postives = 140/178 (78.65%), Query Frame = 0


Query: 1   MANS--IILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELK 60
           MA+S  + +S   L   F + V+S HAAS NDV STIC +TRNP FC +VLKSAGTT+LK
Sbjct: 1   MASSSFLAVSLIVLSVLFFNGVMSMHAASQNDVASTICKKTRNPSFCFDVLKSAGTTDLK 60

Query: 61  GLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLAS 120
           GL+TFTLNLA  +  ESR LAQSLASKA DPKLKERYA+CAE YD+A+ DI+D   NL  
Sbjct: 61  GLSTFTLNLAHDKTVESRALAQSLASKAIDPKLKERYATCAEQYDNAIDDIEDGKVNLGE 120

Query: 121 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 177
           GDYNGVN+ AS AMTEA DC D+FAQPP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFAQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL
Match: A0A6J1GB33 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452548 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.3e-54
Identity = 114/153 (74.51%), Postives = 127/153 (83.01%), Query Frame = 0


Query: 24  HAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLARTRAGESRVLAQSLA 83
           HAAS +D+VSTIC +TRNP FC NVLKSAGTT+LKGLATFTLNLA  + G++R LA SLA
Sbjct: 2   HAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVGQTRALALSLA 61

Query: 84  SKAADPKLKERYASCAENYDDAVGDIDDAGKNLASGDYNGVNVHASGAMTEADDCQDNFA 143
           SKAADPKLKERYA+CAE YDDA  DI+D   +L  GDYNGVN+ AS AMTEA DC D+F 
Sbjct: 62  SKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFT 121

Query: 144 QPPTDPSALSANAKALEDICSIILVIANLLLGR 177
           QPP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 122 QPPKDPSALSGNGKTVEDICSIILVIANLLLGR 154

[Close]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL
Match: A0A6J1IDF4 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111475152 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.8e-54
Identity = 119/178 (66.85%), Postives = 140/178 (78.65%), Query Frame = 0


Query: 1   MANSIILSAYS--LLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELK 60
           MA+S  L+ +   LL  F + ++  HAAS +D++STIC +TRNP FC NVLKSAGTT+LK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDILSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLAS 120
            LATFTLNLA  +  ++R LAQSLASKA+DPKLKERYA+CAE Y +A  DI+D  K+L  
Sbjct: 61  RLATFTLNLAHDKVAQTRALAQSLASKASDPKLKERYATCAEQYGEAADDIEDGKKDLGE 120

Query: 121 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 177
           GDYNGVN+ AS AMTEA DCQDNF QPP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCQDNFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL
Match: A0A6J1EWA8 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111436712 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.8e-54
Identity = 122/178 (68.54%), Postives = 138/178 (77.53%), Query Frame = 0


Query: 1   MANS--IILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELK 60
           MA+S  + +S   L   F + V+S HAAS NDV STIC +TRNP FC +VLKSAGTT+LK
Sbjct: 1   MASSSFLAVSLIVLSVLFFNGVMSMHAASQNDVASTICKKTRNPSFCFDVLKSAGTTDLK 60

Query: 61  GLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLAS 120
           GLATFTLNLA  +  ESR LAQSLASK  DPKLKERYA+CAE YD+A+ DI+D   NL  
Sbjct: 61  GLATFTLNLAHDKTVESRALAQSLASKTIDPKLKERYATCAEQYDNAIDDIEDGKVNLGE 120

Query: 121 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 177
           GDYNGVN+ AS AMTEA DC D+FAQPP DPSA S N K +EDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFAQPPKDPSAFSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lcy05g009660 vs. NCBI nr
Match: XP_038896410.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 243.8 bits (621), Expect = 1.1e-60
Identity = 136/182 (74.73%), Postives = 149/182 (81.87%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSL-----VLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTT 60
           MANSIIL+A SLL F LSL     V+  HAAS N+VVS IC RT NPPFC+N+LKSA TT
Sbjct: 1   MANSIILTASSLLPFVLSLLFFHHVVPMHAASQNNVVSIICQRTGNPPFCSNLLKSARTT 60

Query: 61  ELKGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKN 120
           +LKGLATFTLNLART A +SR LAQ LA KAADPKLKERYASCAE+YDDA GD+DDA   
Sbjct: 61  DLKGLATFTLNLARTNATKSRALAQFLALKAADPKLKERYASCAEHYDDAAGDLDDAKNY 120

Query: 121 LASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 178
           LA+GDYNGVN+ ASGAMTE DDCQDNF Q P D S LS N KALEDICSIILVIAN+LLG
Sbjct: 121 LATGDYNGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKNGKALEDICSIILVIANILLG 180

[Close]

BLAST of Lcy05g009660 vs. NCBI nr
Match: XP_038896411.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 238.4 bits (607), Expect = 4.6e-59
Identity = 132/182 (72.53%), Postives = 148/182 (81.32%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSL-----VLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTT 60
           MANSIIL A SLL F LSL     V+  HAAS NDVVSTIC RTRNPPFC+N+LKSA TT
Sbjct: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60

Query: 61  ELKGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKN 120
           +LKGL TFT+NLA T+A ESR LAQ LASKAADPK KERYASCA++YDDA  D++DA   
Sbjct: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120

Query: 121 LASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 178
           LA+GDY+GVN+ ASGAMTE DDCQDNF Q P D S LS + KALEDICSIILVIAN+LLG
Sbjct: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180

[Close]

BLAST of Lcy05g009660 vs. NCBI nr
Match: XP_023549346.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229.6 bits (584), Expect = 2.2e-56
Identity = 128/181 (70.72%), Postives = 143/181 (79.01%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSL-----VLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTT 60
           MA+S  L+  SLLAF LSL     V+  HAAS +DVVSTIC +TRNP FC N LKSAGTT
Sbjct: 1   MASSSFLAVSSLLAFVLSLLFFNGVVPMHAASPDDVVSTICKKTRNPSFCFNALKSAGTT 60

Query: 61  ELKGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKN 120
           +LKGLATFTLNLA  +A ES  LAQSLASKA DPKLKERY++CAE YDDA  DI+D   N
Sbjct: 61  DLKGLATFTLNLAHDKAVESHALAQSLASKATDPKLKERYSTCAELYDDATDDIEDGKSN 120

Query: 121 LASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLG 177
           L  GDYNGVN+ AS AMTEADDC D+F QPP DPSALS N +A+E+ICSIILVIANLLLG
Sbjct: 121 LREGDYNGVNIKASAAMTEADDCLDSFTQPPKDPSALSGNGEAMENICSIILVIANLLLG 180

[Close]

BLAST of Lcy05g009660 vs. NCBI nr
Match: XP_023521203.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.6 bits (571), Expect = 6.9e-55
Identity = 121/178 (67.98%), Postives = 139/178 (78.09%), Query Frame = 0


Query: 1   MANSIILSAYS--LLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELK 60
           MA+S  L+ +   LL  F + ++   AAS +D+VSTIC +TRNP FC NVLKSAGTT+LK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMQAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLAS 120
           GLATFTLNLA  +  ++R LAQSLASKA DPKLKERYA+CAE YDDA GDI+D   NL  
Sbjct: 61  GLATFTLNLAHDKVAQTRALAQSLASKAVDPKLKERYATCAEQYDDAAGDIEDGKNNLGK 120

Query: 121 GDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGR 177
           GDYNGVN+ AS AMTEA DC D+F QPP DPSALS N K +EDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Lcy05g009660 vs. NCBI nr
Match: XP_022970060.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 223.8 bits (569), Expect = 1.2e-54
Identity = 125/177 (70.62%), Postives = 140/177 (79.10%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTTELKGL 60
           MA+S  L A SL+   + L+L  HAAS NDV STIC +TRNP FC NVLKSAGTT+LKGL
Sbjct: 1   MASSSFL-AVSLIVLSV-LLLPMHAASQNDVASTICKKTRNPSFCFNVLKSAGTTDLKGL 60

Query: 61  ATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLASGD 120
           ATFTLNLA  +  E+R LAQSL SKAADPKLKERYA+CAE YD+A GDI+D   NL  GD
Sbjct: 61  ATFTLNLAHDKVAETRALAQSLTSKAADPKLKERYATCAELYDNAAGDIEDGKVNLGEGD 120

Query: 121 YNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLLLGRI 178
           YNGVN+ A  AMTEA DC D+FAQPP DPSALS N K +EDICSIILVIANLLLGR+
Sbjct: 121 YNGVNIKAFAAMTEAGDCLDSFAQPPKDPSALSGNGKTVEDICSIILVIANLLLGRV 175

[Close]

BLAST of Lcy05g009660 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 94.4 bits (233), Expect = 1.0e-19
Identity = 61/174 (35.06%), Postives = 106/174 (60.92%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSA-GTTELKG 60
           MA ++  +A+     FL L+ S++A +S++ +STIC +T NP FC   L +   +  L+ 
Sbjct: 1   MAANLRNNAFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQA 60

Query: 61  LATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLASG 120
           LA  TL+  + RA ++    QS+     DP+ K  Y SC + Y+ A+G++++A ++LASG
Sbjct: 61  LAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASG 120

Query: 121 DYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 174
           D  G+N+  S A+  AD C D+  +  +  S++  N+K ++++C I LVI+N+L
Sbjct: 121 DGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Lcy05g009660 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 92.0 bits (227), Expect = 5.0e-19
Identity = 61/176 (34.66%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 1   MANSIILSAYSLLAFFLSLVLSTHAASSNDVVSTICSRTRNPPFCTNVLKSAGTT---EL 60
           + N +++S  SL+ F ++  L+   A     +  IC + +N  FCT+ +KS   T   +L
Sbjct: 5   LTNRVLMS--SLMFFVMTGSLNAQVAD----IKAICGKAKNQSFCTSYMKSNPKTSGADL 64

Query: 61  KGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDAGKNLA 120
           + LA  T   A+T A E     QSL   A +P +K+ Y SC ++Y  A+  ++DA ++LA
Sbjct: 65  QTLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLA 124

Query: 121 SGDYNGVNVHASGAMTEADDCQDNFAQPPTDPSALSANAKALEDICSIILVIANLL 174
           SGD  G+N+  S AM     C+ + A    DPSA+  N+   ++IC I+LVI+N++
Sbjct: 125 SGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVK-NSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Lcy05g009660 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.0e-08
Identity = 49/143 (34.27%), Postives = 73/143 (51.05%), Query Frame = 0


Query: 32  VSTICSRTRNPPFCTNVLKSAGTTELKGLATFTLNLARTRAGESRVLAQSLASKAADPKL 91
           + TIC +T NPP C + +    T +LK   +  L LA        + A   A+KAA   +
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILA--------IQASITATKAAMAIV 189

Query: 92  KERYAS-CAENYDDAVGDIDDAGKNLASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPS 151
           ++  AS C E YDDAV +++DA   + S D   VN + S AMT+   C D F +   +P+
Sbjct: 190 EKVDASDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGF-EESGEPN 249

Query: 152 ALSANAKALEDICSIILVIANLL 174
            L+  A  L  + S  L I+ L+
Sbjct: 250 PLAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of Lcy05g009660 vs. TAIR 10
Match: AT1G48010.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.8e-06
Identity = 36/122 (29.51%), Postives = 64/122 (52.46%), Query Frame = 0


Query: 53  GTTELKGLATFTLNLARTRAGESRVLAQSLASKAADPKLKERYASCAENYDDAVGDIDDA 112
           G  +L G+A   ++ A+  A ++    Q LA+   D + KE Y SC++ YD A+   D+A
Sbjct: 54  GKLDLPGVAKCLIHYAQQNALDTHNQLQLLANSTTDNRTKESYISCSKYYDKALYSFDEA 113

Query: 113 GKNLASGDYNGVNVHASGAMTEADDCQDNFAQPPTDPS-ALSANAKALEDICSIILVIAN 172
            K+L   + + +N+  + A   A+DC+    +    P   L      LE++C I+L I++
Sbjct: 114 LKDLKVNNPDYLNIEITAARQNANDCKTLDLKDVFKPDPKLIMKIDFLENVCGILLSISD 173

Query: 173 LL 174
           +L
Sbjct: 174 IL 175

The following BLAST results are available for this feature:

BLAST of Lcy05g009660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	1.1e-26	38.98	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	1.4e-18	35.06	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	7.0e-18	34.66	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Lcy05g009660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I1S1	5.7e-55	70.62	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111469059 PE=4 S...	[more]
A0A6J1F073	7.5e-55	69.10	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111437853 PE=4...	[more]
A0A6J1GB33	1.3e-54	74.51	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452548 PE=4...	[more]
A0A6J1IDF4	2.8e-54	66.85	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111475152 PE=4 S...	[more]
A0A6J1EWA8	2.8e-54	68.54	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111436712 PE=4...	[more]

BLAST of Lcy05g009660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896410.1	1.1e-60	74.73	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_038896411.1	4.6e-59	72.53	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_023549346.1	2.2e-56	70.72	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_023521203.1	6.9e-55	67.98	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970060.1	1.2e-54	70.62	pectinesterase inhibitor-like [Cucurbita maxima]	[more]

BLAST of Lcy05g009660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	1.0e-19	35.06	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	5.0e-19	34.66	pectin methylesterase inhibitor 2	[more]
AT3G36659.1	1.0e-08	34.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G48010.1	2.8e-06	29.51	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..169 e-value: 1.9E-28 score: 110.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..169 e-value: 1.6E-22 score: 80.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..173 e-value: 4.4E-34 score: 115.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..173 e-value: 5.0E-37 score: 129.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..174
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 7..173
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 7..173
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 29..173 e-value: 4.95521E-45 score: 143.717

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g009660.1	Lcy05g009660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity