Homology
BLAST of Lcy05g008790 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 558.9 bits (1439), Expect = 6.0e-158
Identity = 281/507 (55.42%), Postives = 374/507 (73.77%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPP-SIPIIGHLHLLKPPVHRSL 80
ME S +LY + L+++ I++K F S+ +NLPPSP ++P+IGHLHL+ +HRSL
Sbjct: 1 MESSTLLY---TFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSL 60
Query: 81 LALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLG 140
LS+KYG V SL+ G+RLV+VVSS AAEECFTKNDIVFANRP F + + + YN+TT+
Sbjct: 61 YDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMV 120
Query: 141 AAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIV 200
+PYG+HWR LRR++A+EI S+ LN +IR DEV++L+ L + S +DF +VE KS +
Sbjct: 121 GSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKL 180
Query: 201 KELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQ 260
EL+ N+TMRMVAGKRY+G++ +S EA FRAL+ E+FE +S P DF+P L++IDF+
Sbjct: 181 SELSFNVTMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK 240
Query: 261 GFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 320
+ K+ K+ + +D+F Q LI E R K +M+ H L LQESQPEYYTD IIKG
Sbjct: 241 NYEKKVSKISQEMDAFLQRLIHESRINK-----NNVTMIDHLLSLQESQPEYYTDQIIKG 300
Query: 321 LILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLR 380
+I+ ++L G+DT+AVT+EWAMS LLNHP L KAR E+E+Q+G+++L+EE DL KL+YL
Sbjct: 301 IIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLH 360
Query: 381 CIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKP 440
I+ ET RL PA P+L+PH SS DC + GYDVP TILLVNAWAIHRDP WD+P FKP
Sbjct: 361 NIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKP 420
Query: 441 ERFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMT 500
ER G + + +KLM FG+GRR+CPGSG+A RV+GLTLG LIQCF+WKRI ++DM
Sbjct: 421 ERH--GGVELEPSKLM-PFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMA 480
Query: 501 EGKGVSMPKAEPLEALCKAREIARTLI 527
EG G++MPKA+PLEALCK R I ++
Sbjct: 481 EGSGLTMPKAQPLEALCKPRNILHKVV 495
BLAST of Lcy05g008790 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 547.7 bits (1410), Expect = 1.4e-154
Identity = 275/499 (55.11%), Postives = 363/499 (72.75%), Query Frame = 0
Query: 31 FSILSLIAFIIAIKHFLK--SKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLLALSQKYGP 90
+S+LSL +FII IK LK S+ LKNLPP PP+IPIIG+LH LK P+HR+ LSQ YG
Sbjct: 5 YSLLSL-SFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGD 64
Query: 91 VLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGAAPYGDHWR 150
+ SL FGSRLVVVVSS A ECFTKNDI+ ANRP F + + YN+TTLG+A YGDHWR
Sbjct: 65 IFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWR 124
Query: 151 KLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRD---FARVEFKSIVKELTLN 210
LRR++ +++LS+NRLN+ +RRDE RL++KL +D + F +VE + + E+T N
Sbjct: 125 NLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFN 184
Query: 211 ITMRMVAGKRYYGE--EAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQGFMK 270
MRM++GKRYYG+ + ++ EA FR ++ E+ ++ DFLP+L+ +D K
Sbjct: 185 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEK 244
Query: 271 RAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILT 330
R K++ KR ++F +GLI+EHR G I ++ +M+ H LKL ESQPEYY+D +IKGLI
Sbjct: 245 RCKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQG 304
Query: 331 MILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRCIVL 390
M+L G+DT+AVTIEW MS LLNHP VL KA+ EL++Q+G ++L++E DLSKL YL+ I+
Sbjct: 305 MLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIIS 364
Query: 391 ETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPERFE 450
ETLRL+P PLLLPH SS DCTI ++VP DTI+L N W IHRDP W+D +SFKPERFE
Sbjct: 365 ETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE 424
Query: 451 TGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTEGKG 510
E +VNK+M AFGLGRRACPG +A R +G T+GLLIQCF+W+R +++DM EGKG
Sbjct: 425 KEE---EVNKVM-AFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 484
Query: 511 VSMPKAEPLEALCKAREIA 523
++MP PL A+CKA IA
Sbjct: 485 ITMPMKIPLRAMCKALPIA 496
BLAST of Lcy05g008790 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 542.3 bits (1396), Expect = 5.9e-153
Identity = 269/490 (54.90%), Postives = 352/490 (71.84%), Query Frame = 0
Query: 33 ILSLIAFIIAIKHFLK-SKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLLALSQKYGPVLS 92
I+SL II +K F S+ KNLPP P +PIIG+LH LK P+H + LSQKYG + S
Sbjct: 9 IISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 68
Query: 93 LRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGAAPYGDHWRKLR 152
L FGSRLVVVVSS+ A+ECFTKNDIV ANRP F + + YN+TT+ +PYGDHWR LR
Sbjct: 69 LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128
Query: 153 RVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVKELTLNITMRMV 212
R+ ++EILSS+RLN+ IRRDE+ RL++KL + S F VE + + E+T N MRMV
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 188
Query: 213 AGKRYYGE--EAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQGFMKRAKKLI 272
+GKRYYG + ++ EA FR ++ E+ ++ DFL L++ DF G KR KK+
Sbjct: 189 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKIS 248
Query: 273 KRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILGGS 332
KR D+F QGLIDEHR GK ++ N+M+ H L Q+SQPEYYTD IIKGL++ M+L G+
Sbjct: 249 KRTDAFLQGLIDEHRFGKRNS----NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 308
Query: 333 DTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRCIVLETLRLY 392
DT++VTIEWAMSNLLNHP ++ KA+ EL++ +G D+ ++E D+SKL YL+ IV ETLRL+
Sbjct: 309 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 368
Query: 393 PAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPERFETGEGDR 452
A PLL+PH SS D ++ GY++P +TIL+VNAW IHRDP LW DP FKPERFE +
Sbjct: 369 AAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK---EG 428
Query: 453 DVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTEGKGVSMPKA 512
+VNKL ++FGLGRRACPG ++ R GLTLGLLIQCF+WKRI +++DM E KG++ K
Sbjct: 429 EVNKL-LSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKK 488
Query: 513 EPLEALCKAR 520
L A+CK R
Sbjct: 489 TSLNAMCKVR 490
BLAST of Lcy05g008790 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 538.5 bits (1386), Expect = 8.5e-152
Identity = 263/501 (52.50%), Postives = 363/501 (72.46%), Query Frame = 0
Query: 24 SIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLLALS 83
S++ YS F + F I I +++ KNLPP PPS+PIIG+LH LK P+HR+ LS
Sbjct: 5 SLLSYSVFYLALFFIFNIVI----RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 64
Query: 84 QKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGAAPY 143
+KYG V SL FGSRLVVVVSS ++CFTKND+V ANRP F + + YN+TTLG+ Y
Sbjct: 65 EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 124
Query: 144 GDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVKELT 203
G+HWR LRR++AL++LS++R+N+ IRRDE +RL+ +L DSS +FA +E S + ++T
Sbjct: 125 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 184
Query: 204 LNITMRMVAGKRYYGE--EAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQGF 263
N MRM++GKRYYGE + ++ EA+ FR +V EL + + ++ DF+P+L+++DF+
Sbjct: 185 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 244
Query: 264 MKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLI 323
KR K + + D+F +GLI+EHRT K E N+M+ H L LQ+SQPEYYTD IIKGL
Sbjct: 245 EKRLKDISGKTDAFLRGLIEEHRTKK----ERANTMIDHLLNLQDSQPEYYTDQIIKGLA 304
Query: 324 LTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRCI 383
L M+L G+D++AVT+EW+MSNLLNHP VL K + EL++ +G D+L++E+DL KL+YL+ +
Sbjct: 305 LAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNV 364
Query: 384 VLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPER 443
+ ETLRLY PLLLPH +S +C I GY VP DTI+L+NAWAIHRDP LW + +FKPER
Sbjct: 365 INETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPER 424
Query: 444 FE-TGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 503
FE GE ++ +IAFG+GRRACPG G+A R + +TL LLIQCFDWK I G ++D+ E
Sbjct: 425 FEKKGELEK-----LIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 484
Query: 504 GKGVSMPKAEPLEALCKAREI 522
G ++ K PL+A+CK+R +
Sbjct: 485 RDGFTLTKLVPLKAMCKSRPV 492
BLAST of Lcy05g008790 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 526.6 bits (1355), Expect = 3.3e-148
Identity = 259/500 (51.80%), Postives = 360/500 (72.00%), Query Frame = 0
Query: 25 IILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLLALSQ 84
I+ Y +S+ L F I I+ +S+ KNLPP P S+PIIG+LH LK P++R+ AL++
Sbjct: 3 ILSYLCYSLFYLSIFFI-IRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTE 62
Query: 85 KYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGAAPYG 144
KYG V+SL FGSRLVVVVSS+ +ECFTKND+V ANRP F + + YN+TTLG+ YG
Sbjct: 63 KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122
Query: 145 DHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVKELTL 204
+HWR LRR+++L++LS++R+N IRRDE +RL+KKL DSS FA VE ++T
Sbjct: 123 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 182
Query: 205 NITMRMVAGKRYYGEEA--AESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQGFM 264
N MRM++GKRYYG++ +E EA+ FR +V EL + + ++ DF+P+LK++DF+
Sbjct: 183 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 242
Query: 265 KRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLIL 324
KR K++ ++ D F GL+ E R S +E N+M+ H L +QESQPEYYTD IIKGL L
Sbjct: 243 KRVKRIGEKNDVFLSGLLQEQR----SKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 302
Query: 325 TMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRCIV 384
M+L G+D++AVT+EW MSN+LN+P VL K R E+++ +G D+L++E+DL KL+YLR ++
Sbjct: 303 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 362
Query: 385 LETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPERF 444
ETLRLY PLLLPH ++ +C + GY VP DTI+L+NAWAIHRDP W + +FKPERF
Sbjct: 363 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 422
Query: 445 E-TGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTEG 504
+ GE ++ MIAFG+GRRACPG G+A R + +TL LL+QCFDWKRI +++DM+E
Sbjct: 423 DKKGELEK-----MIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSER 482
Query: 505 KGVSMPKAEPLEALCKAREI 522
G +M K PL+A+CK R +
Sbjct: 483 DGFTMTKLLPLKAMCKTRPV 492
BLAST of Lcy05g008790 vs. ExPASy TrEMBL
Match:
A0A6J1H614 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=3 SV=1)
HSP 1 Score: 855.5 bits (2209), Expect = 1.2e-244
Identity = 428/506 (84.58%), Postives = 462/506 (91.30%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
M+DS LYSA IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MDDSTFLYSAL----CIALVVAISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRL KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+VDELFEF L+ YPADFLPILKYID QG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGYDVP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+G+ + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GKEVDMTE
Sbjct: 421 RFEGGDGEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. ExPASy TrEMBL
Match:
A0A6J1KS03 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 SV=1)
HSP 1 Score: 852.0 bits (2200), Expect = 1.3e-243
Identity = 425/506 (83.99%), Postives = 462/506 (91.30%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
M+DS LYS IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MDDSTFLYSVL----CIALVVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRLV KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+V+ELFEF L+ YPADFLPILKYIDFQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT ++ N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGY+VP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+ + + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GKEVDMTE
Sbjct: 421 RFEGGDSEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. ExPASy TrEMBL
Match:
A0A5D3DLU7 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001700 PE=3 SV=1)
HSP 1 Score: 838.2 bits (2164), Expect = 1.9e-239
Identity = 418/508 (82.28%), Postives = 459/508 (90.35%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIA-IKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSL 80
MEDSI+ YSAFSILS+ ++A IK+ LK S KNLPPSPPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 81 LALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLG 140
LSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 141 AAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIV 200
AAPYGDHWRKLRRVS LEIL S+RLNAN+ IRRDEVRR VKK+ S F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 201 KELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQ 260
KELT+NITMRMVAGKRYYGEEAA+SSEA TF+A+V ELFEFTLSSYPADFLPILKYID Q
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 261 GFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 320
GFMKRAKKLI RVD+FWQGLIDEHR G+ISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 321 LILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLR 380
LILTMILGGSDTTAVTIEWAMSNLLNHP+VL KARAE+ESQL D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 381 CIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKP 440
CIVLETLRLYPAGPLLLPH+SSADC+ISGY++P DTILLVNAWAIHRDPTLW+DP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 441 ERFETGEGDRDV-NKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDM 500
ERFE G+ D NKLMIAFGLGRRACPG+GMAHRV+GL LG LIQCFDWK I+GKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 501 TEGKGVSMPKAEPLEALCKAREIARTLI 527
EGKGVSMPKA+PLEALCKAREIA L+
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Lcy05g008790 vs. ExPASy TrEMBL
Match:
A0A1S3CAW8 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1)
HSP 1 Score: 838.2 bits (2164), Expect = 1.9e-239
Identity = 418/508 (82.28%), Postives = 459/508 (90.35%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIA-IKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSL 80
MEDSI+ YSAFSILS+ ++A IK+ LK S KNLPPSPPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 81 LALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLG 140
LSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 141 AAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIV 200
AAPYGDHWRKLRRVS LEIL S+RLNAN+ IRRDEVRR VKK+ S F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 201 KELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQ 260
KELT+NITMRMVAGKRYYGEEAA+SSEA TF+A+V ELFEFTLSSYPADFLPILKYID Q
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 261 GFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 320
GFMKRAKKLI RVD+FWQGLIDEHR G+ISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 321 LILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLR 380
LILTMILGGSDTTAVTIEWAMSNLLNHP+VL KARAE+ESQL D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 381 CIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKP 440
CIVLETLRLYPAGPLLLPH+SSADC+ISGY++P DTILLVNAWAIHRDPTLW+DP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 441 ERFETGEGDRDV-NKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDM 500
ERFE G+ D NKLMIAFGLGRRACPG+GMAHRV+GL LG LIQCFDWK I+GKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 501 TEGKGVSMPKAEPLEALCKAREIARTLI 527
EGKGVSMPKA+PLEALCKAREIA L+
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Lcy05g008790 vs. ExPASy TrEMBL
Match:
A0A097IYP1 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1)
HSP 1 Score: 823.5 bits (2126), Expect = 4.9e-235
Identity = 410/508 (80.71%), Postives = 455/508 (89.57%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIA-IKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSL 80
MEDSI+ YSAFSILS+ ++A IK+ LK S KNLPPSPPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 81 LALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLG 140
LSQKYGPVLSLRFGSR VVVV+S++AAEECF KNDI+FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 141 AAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIV 200
AAPYGDHWRKLRRVS LEIL S+RLNAN+ IRRDEVRR +KK+ S F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 201 KELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQ 260
KELT+N+TMRMVAGKRY+GEEAA+SSEA TF+A+V ELFEFTLSSYPADFLPILKYID Q
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 261 GFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 320
GFMKRAKKLI RVD+FWQGLIDEHR G+ISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 321 LILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLR 380
LILTMILGGSDTTAVTIEWAMSNLLNHP+VL KARAE+ESQLG D+L+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 381 CIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKP 440
I+LETLRLYPAGPLLLPH+SSADC ISGYD+P DT+LLVNAWAIHRDP LW+DP SFKP
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 441 ERFETGEGDRD-VNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDM 500
ERFE G+ D NKLMIAFGLGRRACPG+GMA+RV+GL LG LIQCFDWK I+GKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 501 TEGKGVSMPKAEPLEALCKAREIARTLI 527
EGKGVSMPKA+PLEALCKAREIA L+
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Lcy05g008790 vs. NCBI nr
Match:
XP_038907094.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 869.4 bits (2245), Expect = 1.6e-248
Identity = 438/507 (86.39%), Postives = 464/507 (91.52%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
MEDSI+ YSA SILS+ + IKH LKS KNLPP PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSSM AAEECFTKNDI+FANRPDFAVSRCLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILSSNRLN N IRRDEVRR+++K+ S DF +VEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEEAA+SSEA TF+A+V+ELFEFTLSSYPADFLPILKYID QG
Sbjct: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLI RVD+FWQGLIDEHR G+ISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL
Sbjct: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTMILGGSDTTAVTIEWAMSNLLNHP+VL KARAELESQLG DQLLEETDLSKLSYLRC
Sbjct: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCTI GYDVP DTILLVNAWAIHRDPTLW+DPMSFKPE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
Query: 441 RFETGEGDRD-VNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMT 500
RFE G+ D +KLMIAFGLGRRACPG+GMAHRVLGL+LGLLIQCFDWK IEGKEVDMT
Sbjct: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
Query: 501 EGKGVSMPKAEPLEALCKAREIARTLI 527
EG GVSMPKA+PLEALCKAREIA L+
Sbjct: 481 EGIGVSMPKAQPLEALCKAREIATKLL 507
BLAST of Lcy05g008790 vs. NCBI nr
Match:
XP_023513972.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 857.8 bits (2215), Expect = 4.8e-245
Identity = 428/506 (84.58%), Postives = 463/506 (91.50%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
MED+ LYS IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MEDTTFLYSVLG----IALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRLV KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+VDELFEF L+ YPADFLPILKYIDFQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGYDVP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+G+ + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GK+VDMTE
Sbjct: 421 RFEGGDGEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKDVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. NCBI nr
Match:
XP_022959856.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])
HSP 1 Score: 855.5 bits (2209), Expect = 2.4e-244
Identity = 428/506 (84.58%), Postives = 462/506 (91.30%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
M+DS LYSA IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MDDSTFLYSAL----CIALVVAISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRL KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+VDELFEF L+ YPADFLPILKYID QG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGYDVP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+G+ + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GKEVDMTE
Sbjct: 421 RFEGGDGEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. NCBI nr
Match:
KAG7025244.1 (Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 855.5 bits (2209), Expect = 2.4e-244
Identity = 428/506 (84.58%), Postives = 462/506 (91.30%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
M+DS LYS IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MDDSTFLYSVL----CIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRLV KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+VDELFEF L+ YPADFLPILKYID QG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGYDVP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+G+ + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GKEVDMTE
Sbjct: 421 RFEGGDGEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. NCBI nr
Match:
XP_023005007.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 852.0 bits (2200), Expect = 2.6e-243
Identity = 425/506 (83.99%), Postives = 462/506 (91.30%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPSIPIIGHLHLLKPPVHRSLL 80
M+DS LYS IA ++AI + LK KS KNLPP+PPSIPIIGHLHLLKPPVHRSLL
Sbjct: 1 MDDSTFLYSVL----CIALVVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
Query: 81 ALSQKYGPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLGA 140
LSQKYGPVLSLRFGSRLVVVVSS+DAAEECFTKND+VFANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
Query: 141 APYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVK 200
APYGDHWRKLRRVSALEILS+NRLN N AIRRDEVRRLV KL + S DFARVEFKS+VK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180
Query: 201 ELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQG 260
ELTLNITMRMVAGKRYYGEE ESSEA TFRA+V+ELFEF L+ YPADFLPILKYIDFQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQG 240
Query: 261 FMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 320
FMKRAKKLIKRVD+FWQG++DEHRT ++ N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
Query: 321 ILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLRC 380
ILTM+LGGSDTTAVTIEWAMSNLLNHP VL KARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
Query: 381 IVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPE 440
IVLETLRLYPAGPLLLPH+SSADCT+SGY+VP DTILLVNAWAIHRDPTLW+DP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
Query: 441 RFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTE 500
RFE G+ + + +KLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWK I+GKEVDMTE
Sbjct: 421 RFEGGDSEAN-SKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE 480
Query: 501 GKGVSMPKAEPLEALCKAREIARTLI 527
GKGVSMPKAEPLEALCK R IA L+
Sbjct: 481 GKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of Lcy05g008790 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 545.0 bits (1403), Expect = 6.4e-155
Identity = 279/503 (55.47%), Postives = 372/503 (73.96%), Query Frame = 0
Query: 21 MEDSIILYS-AFSILSLIAFIIAIKHFLKSKSLKNLPPSPP-SIPIIGHLHLLKPPVHRS 80
ME +++S F +LSLI I K K NLPPSP S+P+IGHL LLKPP+HR+
Sbjct: 1 METKTLIFSILFVVLSLIYLI------GKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRT 60
Query: 81 LLALSQKY--GPVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHT 140
L+LSQ P+ SLR G+RLV V SS AEECFTKND+V ANRP+F +++ ++Y++T
Sbjct: 61 FLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYT 120
Query: 141 TLGAAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFK 200
T+ AA YGDHWR LRR+ ++EI S++RLN+ +IR+DE+RRLV +L R+ S++F +V+ K
Sbjct: 121 TMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMK 180
Query: 201 SIVKELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYI 260
S++ +LT N +RMVAGKRYYG+ + EA R L+ ++ + D+LP+L+ +
Sbjct: 181 SMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV 240
Query: 261 DFQGFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDI 320
+ R KKL R+D F QGL+DE R K E N+M+ H L LQESQP+Y+TD I
Sbjct: 241 --SDYETRVKKLAGRLDEFLQGLVDEKREAK----EKGNTMIDHLLTLQESQPDYFTDRI 300
Query: 321 IKGLILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLS 380
IKG +L +IL G+DT+AVT+EWA+SN+LNHP VLNKAR E++ ++G D+L++E+D+S L
Sbjct: 301 IKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLP 360
Query: 381 YLRCIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMS 440
YL+ IV ETLRLYPA P+LLPH +S DC ++GYD+P TILL N WAIHRDP LWDDPMS
Sbjct: 361 YLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMS 420
Query: 441 FKPERFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEV 500
FKPERFE + + KLM FGLGRRACPGSG+AHR++ LTLG LIQC +W++I G+EV
Sbjct: 421 FKPERFEK---EGEAQKLM-PFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEV 480
Query: 501 DMTEGKGVSMPKAEPLEALCKAR 520
DM+EGKGV+MPKA+PLEA+C+AR
Sbjct: 481 DMSEGKGVTMPKAKPLEAMCRAR 486
BLAST of Lcy05g008790 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 531.6 bits (1368), Expect = 7.3e-151
Identity = 278/510 (54.51%), Postives = 368/510 (72.16%), Query Frame = 0
Query: 21 MEDSIILYS-AFSILSLIAFIIAIKHFLKSKSLKNLPPSPP-SIPIIGHLHLLKPPVHRS 80
ME +++++ F +LSLI I IK L NLPPSP ++P+IGHL LLKPP+HR
Sbjct: 1 MEALMLIFTFCFIVLSLIFLIGRIKRKL------NLPPSPAWALPVIGHLRLLKPPLHRV 60
Query: 81 LLALSQKYG--PVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHT 140
L++SQ G P++SLR G+RL+ VVSS AEECFTKND++ ANR ++ +SY ++
Sbjct: 61 FLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNS 120
Query: 141 TLGAAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFK 200
T+ +A Y +HWR LRR+ ALEI S++RLN+ +IRRDE+RRL+ +L R+SS F +VE K
Sbjct: 121 TVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMK 180
Query: 201 SIVKELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYI 260
S+ +LT N +RM+AGK YYG+ + EA R L+ E + AD++PIL +I
Sbjct: 181 SMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI 240
Query: 261 DFQGFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDI 320
+ R KKL R+D F QGL+DE R GK E+ EN+MV H L LQE+QPEYY D I
Sbjct: 241 TYS--ETRIKKLAGRLDEFLQGLVDEKREGK---EKKENTMVDHLLCLQETQPEYYMDRI 300
Query: 321 IKGLILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLS 380
IKG +L++I GG+DTTAVT+EWA+S+LLN+P VLNKAR E++ +G D+LLEE+D+ L
Sbjct: 301 IKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLP 360
Query: 381 YLRCIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMS 440
YL+ IV ETLRLYPA P+LLPH +S DC + GYD+P T+LL NAWAIHRDP LWDDP S
Sbjct: 361 YLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTS 420
Query: 441 FKPERFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEV 500
FKPERFE EG+ K ++ FGLGRRACPGSG+A R++ L+LG LIQCF+W+RI +EV
Sbjct: 421 FKPERFEK-EGEA---KKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEV 480
Query: 501 DMTEGKGVSMPKAEPLEALCKAREIARTLI 527
DMTEG G++MPKA PLEA+C+AR+ ++
Sbjct: 481 DMTEGPGLTMPKARPLEAMCRARDFVGKIL 495
BLAST of Lcy05g008790 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 527.7 bits (1358), Expect = 1.1e-149
Identity = 268/508 (52.76%), Postives = 361/508 (71.06%), Query Frame = 0
Query: 23 DSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPP-SIPIIGHLHLLKPPVHRSLLA 82
+ I++ S S+ +L F++ +SK NLPPSP S+P+IGHLHLLKPP+HR+ L+
Sbjct: 4 NQILILSFLSLFTLAIFLLT-----RSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLS 63
Query: 83 LSQKYG--PVLSLRFGSRLVVVVSSMDAAEECFTKNDIVFANRPDFAVSRCLSYNHTTLG 142
LS+ G PV LR G+RLV V+SS AEECFTKND+V ANRP F +S+ L YN T L
Sbjct: 64 LSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLL 123
Query: 143 AAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIV 202
+A YGDHWR LRR++A+EI S++RLN+ IR+DE+RRL+ L RDS F VE K+++
Sbjct: 124 SASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLL 183
Query: 203 KELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQ 262
L N T+RM+AGKRY+GE ++ +A + LV E + P D+L IL+++
Sbjct: 184 TNLASNTTIRMLAGKRYFGE---DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--S 243
Query: 263 GFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 322
+ KR K L R D+F Q L+DE R K E +M+ H L LQ+ QP+YYTD IIKG
Sbjct: 244 SYEKRIKNLGNRFDTFLQKLVDEKRAEK----EKGETMIDHLLALQDIQPDYYTDVIIKG 303
Query: 323 LILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLSKLSYLR 382
+ILT+I+ G+DT++VT+EWAMSNLLNHP +L KAR E++ ++G D+L++E+D+ LSYL+
Sbjct: 304 IILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQ 363
Query: 383 CIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKP 442
IVLETLR+YPA PLLLPH SS DC + GYD+PS T++L NAWA+HRDP +W+DP FKP
Sbjct: 364 SIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKP 423
Query: 443 ERFE-TGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDM 502
ERFE GE ++ +I+FG+GRRACPG+G+AHR++ LG L+QCF+W+R+ VDM
Sbjct: 424 ERFEKEGEAEK-----LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDM 483
Query: 503 TEGKGVSMPKAEPLEALCKAREIARTLI 527
TE KG ++PKA PL A+CKAR I LI
Sbjct: 484 TEDKGATLPKAIPLRAMCKARSIVDKLI 492
BLAST of Lcy05g008790 vs. TAIR 10
Match:
AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )
HSP 1 Score: 525.0 bits (1351), Expect = 6.9e-149
Identity = 265/501 (52.89%), Postives = 359/501 (71.66%), Query Frame = 0
Query: 33 ILSLIAFIIAIKHFLKSKSLKNLPPSPP-SIPIIGHLHLLKPP-VHRSLLALSQKYGPVL 92
+ +L+A I L +K KNLPPSP PIIGHLHLLK P +HR+L LS GPV
Sbjct: 8 LTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPVF 67
Query: 93 SLRFGSRLVVVVSSMDAAEECF-TKNDIVFANRPDFAVSRCLSYNHTTLGAAPYGDHWRK 152
SLR GSRL V++SS AAEECF TKNDIV ANRP F + + ++Y++T++ APYGDHWR
Sbjct: 68 SLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRN 127
Query: 153 LRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARVEFKSIVKELTLNITMR 212
LRR++ALE+ S+NRLNA+ IR DEV+ L++KL S A+VE + ++ LTLN+ MR
Sbjct: 128 LRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIMR 187
Query: 213 MVAGKRYYGE-EAAESSEATTFRALVDELFEFTLSSYPADFLPILKYIDFQGFMKRAKKL 272
M+ GKR++ E + ++ + FR LV E+ E + + PADFLP L++ D++G +KRAK++
Sbjct: 188 MMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKRI 247
Query: 273 IKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILGG 332
+R+DS QG +DEHR K E +N+M+AH L QE +P Y+D IKGLIL M++GG
Sbjct: 248 GERMDSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILMMVVGG 307
Query: 333 SDTTAVTIEWAMSNLLNHPAVLNKARAELESQL---GADQLLEETDLSKLSYLRCIVLET 392
+DT+A+T+EWAMSNLLNHP +L R +++Q+ + +LL+E DL ++YL+ +V ET
Sbjct: 308 TDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSET 367
Query: 393 LRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDDPMSFKPERFETG 452
LRLYP PL++PH S+DC I G++VP DTI+LVN WAIHRDP++WDDP SFKPERFE
Sbjct: 368 LRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEGS 427
Query: 453 EGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEGKEVDMTEGKGVS 512
+ N M+ FGLGRRACPG +A+RV+GL LG +IQCF+W+ G +VDMTEG G+S
Sbjct: 428 DQFGHYNGKMMPFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPGLS 487
Query: 513 MPKAEPLEALCKAREIARTLI 527
+PKAEPL C+ RE+A L+
Sbjct: 488 LPKAEPLVVTCRTREMASELL 507
BLAST of Lcy05g008790 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 522.3 bits (1344), Expect = 4.5e-148
Identity = 280/513 (54.58%), Postives = 358/513 (69.79%), Query Frame = 0
Query: 21 MEDSIILYSAFSILSLIAFIIAIKHFLKSKSLKNLPPSPPS-IPIIGHLHLLKPPVHRSL 80
ME++ I +SI SLI II+ K FLK K +NLPPSPP +PIIGHL LLKPP+HR+L
Sbjct: 1 MEETNIRVVLYSIFSLIFLIISFK-FLKPKK-QNLPPSPPGWLPIIGHLRLLKPPIHRTL 60
Query: 81 LALSQKY-----GPVLSLRFGSRLVVVVSSMD-AAEECFTKNDIVFANRPDFAVSRCLSY 140
+ S+ G V+SLR GSRLV VVSS AAEECF KND+V ANRP + + + Y
Sbjct: 61 RSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGY 120
Query: 141 NHTTLGAAPYGDHWRKLRRVSALEILSSNRLNANFAIRRDEVRRLVKKLCRDSSRDFARV 200
N+T + AAPYGDHWR LRR+ +EI S++RLN +R DEVRRL+ +L R + V
Sbjct: 121 NNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVV 180
Query: 201 EFKSIVKELTLNITMRMVAGKRYYGEEAAESSEATTFRALVDELFEFTLSSYPADFLPIL 260
E K ++ +LT N MRM+ GKRYYGEE + EA R LV ++ T S D++PIL
Sbjct: 181 ELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPIL 240
Query: 261 KYIDFQGFMKRAKKLIKRVDSFWQGLIDEHRTGKISNEEMENSMVAHFLKLQESQPEYYT 320
+ F + R KKL + D F QGLID+ R +E +M+ H L LQ+S EYYT
Sbjct: 241 RL--FSSYENRVKKLGEETDKFLQGLIDDKR----GQQETGTTMIDHLLVLQKSDIEYYT 300
Query: 321 DDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPAVLNKARAELESQLGADQLLEETDLS 380
D IIKG+IL M++ G++T+AVT+EWA+SNLLNHP V++KAR E+++++G D+L+EE DLS
Sbjct: 301 DQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLS 360
Query: 381 KLSYLRCIVLETLRLYPAGPLLLPHRSSADCTISGYDVPSDTILLVNAWAIHRDPTLWDD 440
+L YL+ IVLETLRL+PA PLL+PH +S DC I YD+P T LLVNAWAIHRDP WDD
Sbjct: 361 ELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDD 420
Query: 441 PMSFKPERFETGEGDRDVNKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKRIEG 500
P SFKPERFE E + KL +AFGLGRRACPGSG+A R++GL LG LIQCF+W+R+
Sbjct: 421 PDSFKPERFEKEE---EAQKL-LAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGN 480
Query: 501 KEVDMTEGKGVSMPKAEPLEALCKAREIARTLI 527
EVDM EG G ++PKA PL+A+CKAR +I
Sbjct: 481 VEVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 6.0e-158 | 55.42 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ9 | 1.4e-154 | 55.11 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| Q6WNQ8 | 5.9e-153 | 54.90 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| P93147 | 8.5e-152 | 52.50 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
| Q6WNR0 | 3.3e-148 | 51.80 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1H614 | 1.2e-244 | 84.58 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=... | [more] |
| A0A6J1KS03 | 1.3e-243 | 83.99 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 ... | [more] |
| A0A5D3DLU7 | 1.9e-239 | 82.28 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
| A0A1S3CAW8 | 1.9e-239 | 82.28 | isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1 | [more] |
| A0A097IYP1 | 4.9e-235 | 80.71 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37370.1 | 6.4e-155 | 55.47 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37360.1 | 7.3e-151 | 54.51 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
| AT4G37330.1 | 1.1e-149 | 52.76 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
| AT4G37310.1 | 6.9e-149 | 52.89 | cytochrome P450, family 81, subfamily H, polypeptide 1 | [more] |
| AT5G36220.1 | 4.5e-148 | 54.58 | cytochrome p450 81d1 | [more] |
