Homology
BLAST of Lcy05g007860 vs. ExPASy Swiss-Prot
Match:
P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)
HSP 1 Score: 265.0 bits (676), Expect = 1.2e-69
Identity = 142/334 (42.51%), Postives = 201/334 (60.18%), Query Frame = 0
Query: 4 AKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASKEF 63
A FVSNW ++V ES++ P +KR VP+ +P++DL H LL+++I A ++F
Sbjct: 2 ATFVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDF 61
Query: 64 GFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAK--------ECSRDPNKSCRVYTSSE 123
G FQVINHG +++ ETM++ KEF A+ EK K + + ++Y E
Sbjct: 62 GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121
Query: 124 NYFKEQTHCWRDALIFNCHPLDKYL-HFWPQNPPKYREVVGAYCVAMRKLVLEILELISE 183
E+ W+D L CHPLD+ L + WP+ P KYREVV Y V +RKL + +L+ I E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181
Query: 184 GLGLGRGYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQ--VL 243
GLGL GY E S+ ++L N+YPPCP+PS TLG H D +LIT+L QD+ GLQ ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241
Query: 244 KDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVT 303
KD WI V PI AFVVN+G L+VI+N K + + HR VT+ R S+ L+ P T
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301
Query: 304 MEPAKCLINEANPPRYRSLNFKDFQRNYLPRAAD 327
+EPAK L+N+ NPP Y+ ++ +F YL +D
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332
BLAST of Lcy05g007860 vs. ExPASy Swiss-Prot
Match:
Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)
HSP 1 Score: 264.6 bits (675), Expect = 1.5e-69
Identity = 141/337 (41.84%), Postives = 207/337 (61.42%), Query Frame = 0
Query: 4 AKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASKEF 63
AK +S T+PE+YV P RP V + P++DL++ DR+ LI++I A F
Sbjct: 3 AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62
Query: 64 GFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTH 123
GFFQVINHGV++++++E + + +EF +MS EK K S DP K+ R+ TS N KE+ +
Sbjct: 63 GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122
Query: 124 CWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYL 183
WRD L +C+P+ KY++ WP NPP ++E+V Y +R++ +I ELISE LGL + Y+
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182
Query: 184 G---GEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD--VNGLQVLKDGQWI 243
GE+ ++ + VN+YPPCP P LT GL H DP+ +TIL QD V GLQ+L DGQW
Sbjct: 183 KKVLGEQGQH--MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWF 242
Query: 244 GVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKC 303
V+P +AFV+NIG LQ +SNG K+ HRAVTN + R S+ + P D M PAK
Sbjct: 243 AVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKP 302
Query: 304 L---INEANPPRYRSLNFKDFQRNYLPRAADTKAVMQ 333
L ++ P Y+ + ++ + + R D + ++
Sbjct: 303 LWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336
BLAST of Lcy05g007860 vs. ExPASy Swiss-Prot
Match:
Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)
HSP 1 Score: 262.3 bits (669), Expect = 7.5e-69
Identity = 134/324 (41.36%), Postives = 195/324 (60.19%), Query Frame = 0
Query: 14 ETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASKEFGFFQVINHGV 73
+T+P YV P +RP +V + IPVVDL + DRA ++ + DA + GFFQV+NHG+
Sbjct: 18 DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77
Query: 74 SRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCWRDALIFNC 133
++ M++ +EF + EKAK S DP K R+ TS N KE H WRD L +C
Sbjct: 78 DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137
Query: 134 HPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLG---GEKSEN 193
+PL +++ WP NPP ++E++G YC +R+L + E ISE LGL GY+ GE+ ++
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197
Query: 194 PVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD--VNGLQVLKDGQWIGVHPIDNAFV 253
+ VN+YP CP P LT GL H DP+ +TIL D V GLQVL DG+WI V+P A V
Sbjct: 198 --MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALV 257
Query: 254 VNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRY 313
+NIG LQ +SNGK ++ HRAV N+ R S+ + P + V + PAK LI + +P Y
Sbjct: 258 INIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVY 317
Query: 314 RSLNFKDFQRNYLPRAADTKAVMQ 333
R+ + ++ + + R D + ++
Sbjct: 318 RNYTYDEYYKKFWSRNLDQEHCLE 338
BLAST of Lcy05g007860 vs. ExPASy Swiss-Prot
Match:
Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)
HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 129/327 (39.45%), Postives = 191/327 (58.41%), Query Frame = 0
Query: 13 VETVPESYVYPPEKRPG-NVVVPMEKAIPVVDLTTHD-----RALLIRKIFDASKEFGFF 72
V+ VP +YV P RP + V +IP++DL HD RA +I + A GFF
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74
Query: 73 QVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCWR 132
Q+ NHGV + +++ M +EF S +E+ K S D K+ R+ TS N KE+ WR
Sbjct: 75 QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134
Query: 133 DALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLGGE 192
D L +C+P++ +++ WP P +REV Y ++R LVL +LE ISE LGL + +
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194
Query: 193 KSEN-PVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD-VNGLQVLKDGQWIGVHPID 252
++ + +N+YP CP P LT GL H D +LIT+L QD V+GLQV KDG+WI V+P+
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254
Query: 253 NAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA- 312
N F+VN+G +QVISN K K+ HRAV N+ + R S+ P ++ + PA+ LINE
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314
Query: 313 -NPPRYRSLNFKDFQRNYLPRAADTKA 330
+P YR+ + ++ + A DT++
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338
BLAST of Lcy05g007860 vs. ExPASy Swiss-Prot
Match:
Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)
HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 125/312 (40.06%), Postives = 183/312 (58.65%), Query Frame = 0
Query: 15 TVPESYVYPPEKRPGNVVVPMEKAI---PVVDLTTHDRALLIRKIFDASKEFGFFQVINH 74
++PES+V+ +K P P KA+ P++DL+ R + R I +A KE GFFQV+NH
Sbjct: 13 SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72
Query: 75 GVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCWRDALIF 134
GVS++V+ + + ++F + A+KA S + +K R++ S Y WRD L
Sbjct: 73 GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRL 132
Query: 135 NC-HPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLGGEKS-E 194
C P+D ++ WP P R+V+ + R + E+L L+ EG+G+ Y G+ S
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192
Query: 195 NPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD-VNGLQVLKDGQWIGVHPIDNAFV 254
N +L +NHYP CPNP LG HCD +LIT+L VNGL+V G WI V P NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252
Query: 255 VNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRY 314
VN G L+V++NG LK+ EHRA+TN+ ++R S+ + P E + PAK +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312
Query: 315 RSLNFKDFQRNY 321
R+ F+DF R Y
Sbjct: 313 RTTMFRDFMRIY 317
BLAST of Lcy05g007860 vs. ExPASy TrEMBL
Match:
A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)
HSP 1 Score: 639.4 bits (1648), Expect = 8.4e-180
Identity = 299/335 (89.25%), Postives = 319/335 (95.22%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP YVYPPEKRPGNVVVPM AIPV+DL+THDR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVSR+VLEET +IFK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPLDKY+HFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYLGGE SENP++LVNHYP CPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIG 336
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIG 335
BLAST of Lcy05g007860 vs. ExPASy TrEMBL
Match:
A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)
HSP 1 Score: 636.3 bits (1640), Expect = 7.1e-179
Identity = 300/339 (88.50%), Postives = 320/339 (94.40%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP Y+YPPEKRPGNVVVPM AIPVVDL+T DR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVSR+VLEET +IFK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPL+KY+HFWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYL E SENP++LVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG N S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339
BLAST of Lcy05g007860 vs. ExPASy TrEMBL
Match:
A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)
HSP 1 Score: 630.2 bits (1624), Expect = 5.1e-177
Identity = 294/338 (86.98%), Postives = 316/338 (93.49%), Query Frame = 0
Query: 2 GDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASK 61
G AKFVSNWE VE+VPESYVYPPEKRPGN+VVPM KAIPV+DL+ DR LL+RKI DASK
Sbjct: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
Query: 62 EFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQ 121
EFGFFQ+INHGVS+KV EETM+IFKEFHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
Query: 122 THCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRG 181
HCWRDALIFNCHPL+KY+HFWPQ PPKYREVVGAYC+AMRKLVLEILEL+SEGLGLG+G
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGVH 241
Y GGE SENP+LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQDVNGLQVLKDGQWIGV
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
Query: 302 EANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
EANPPRYRSLNFKDFQRNYLPRA DTKAVMQYI N S
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of Lcy05g007860 vs. ExPASy TrEMBL
Match:
A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)
HSP 1 Score: 627.1 bits (1616), Expect = 4.3e-176
Identity = 295/338 (87.28%), Postives = 316/338 (93.49%), Query Frame = 0
Query: 2 GDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASK 61
GDAKFVSNWE E+VPESYVYPPEKRPGNVVVPM KAIPV+DL+ DRALL+RKI ASK
Sbjct: 4 GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63
Query: 62 EFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQ 121
E GFFQ+INHGVS++V E TM+IFKEFHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64 ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123
Query: 122 THCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRG 181
HCWRDALIFNCHPL+KY+HFWPQNPPKYREVVGAYCVAMRKLVLEILEL+SEGLGLG+G
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGVH 241
YLGGE SENP+LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQDVNGLQVLKDGQWIGV
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PID AFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE TMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303
Query: 302 EANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
EANPPRYRSLNFKDFQRNYLPRA DTKAVMQYI N S
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of Lcy05g007860 vs. ExPASy TrEMBL
Match:
A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)
HSP 1 Score: 605.5 bits (1560), Expect = 1.3e-169
Identity = 279/340 (82.06%), Postives = 309/340 (90.88%), Query Frame = 0
Query: 1 MGD-AKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDA 60
MGD FVSNW GVE+VPESYVYPPEKRPGNV VP+E AIPVVDLT HDRA L+ KI DA
Sbjct: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
Query: 61 SKEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFK 120
S+EFGFFQVINHGVS+ ++EETM++F+EFHAMSG EK +ECS+DPN+SCRVYTSSEN+ K
Sbjct: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
Query: 121 EQTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLG 180
E+THCWRDALIFNCHPLDKY+HFWPQNP YREVVGAYC+ MRKLV+EILELISEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
Query: 181 RGYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIG 240
+GY G E SENP+LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQ +NGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
Query: 241 VHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL 300
VHP+DNAFVVNIGFVLQV+SNGKLKA EHRAV NAK+SRQS+TYLVYPKD+ TMEPAKC+
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
Query: 301 INEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
INEANP Y SL FKDFQRNYLP AADTKAV+QYI +HS
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340
BLAST of Lcy05g007860 vs. NCBI nr
Match:
XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 300/339 (88.50%), Postives = 321/339 (94.69%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP YVYPPEKRPGNVVVPM IPV+DL+T DR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVS++VLEET +IFK+FHAMSGAEKAKECSRDPNKSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPLDKY+HFWPQNPPKYREVVGAYCV MR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYLGGE SENP++LVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG + S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
BLAST of Lcy05g007860 vs. NCBI nr
Match:
KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 299/335 (89.25%), Postives = 319/335 (95.22%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP YVYPPEKRPGNVVVPM AIPV+DL+T DR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVSR+VLEET +IFK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPLDKY+HFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYLGGE SENP++LVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIG 336
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIG 335
BLAST of Lcy05g007860 vs. NCBI nr
Match:
XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])
HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 299/335 (89.25%), Postives = 319/335 (95.22%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP YVYPPEKRPGNVVVPM AIPV+DL+THDR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVSR+VLEET +IFK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPLDKY+HFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYLGGE SENP++LVNHYP CPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIG 336
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIG 335
BLAST of Lcy05g007860 vs. NCBI nr
Match:
KAG7025486.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 638.3 bits (1645), Expect = 3.9e-179
Identity = 299/335 (89.25%), Postives = 319/335 (95.22%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP YVYPPEKRPGNVVVPM AIPV+DL+T DR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGV R+VLEET +IFK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPLDKY+HFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYLGGE SENP++LVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIG 336
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIG 335
BLAST of Lcy05g007860 vs. NCBI nr
Match:
XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])
HSP 1 Score: 636.3 bits (1640), Expect = 1.5e-178
Identity = 300/339 (88.50%), Postives = 320/339 (94.40%), Query Frame = 0
Query: 1 MGDAKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDAS 60
MG+ KFVSNWEGV++VP Y+YPPEKRPGNVVVPM AIPVVDL+T DR LL+RKI DAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60
Query: 61 KEFGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKE 120
+EFGFFQVINHGVSR+VLEET +IFK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGR 180
QTHCWRDALIFNCHPL+KY+HFWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
GYL E SENP++LVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRSLNFKDFQRNYLPRAADTKAVMQYIGFNHS 340
NEANPPRYR+L FKDFQRNYLPRA+DTKAVM+ IG N S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339
BLAST of Lcy05g007860 vs. TAIR 10
Match:
AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 264.6 bits (675), Expect = 1.1e-70
Identity = 141/337 (41.84%), Postives = 207/337 (61.42%), Query Frame = 0
Query: 4 AKFVSNWEGVETVPESYVYPPEKRPGNVVVPMEKAIPVVDLTTHDRALLIRKIFDASKEF 63
AK +S T+PE+YV P RP V + P++DL++ DR+ LI++I A F
Sbjct: 3 AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62
Query: 64 GFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTH 123
GFFQVINHGV++++++E + + +EF +MS EK K S DP K+ R+ TS N KE+ +
Sbjct: 63 GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122
Query: 124 CWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYL 183
WRD L +C+P+ KY++ WP NPP ++E+V Y +R++ +I ELISE LGL + Y+
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182
Query: 184 G---GEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD--VNGLQVLKDGQWI 243
GE+ ++ + VN+YPPCP P LT GL H DP+ +TIL QD V GLQ+L DGQW
Sbjct: 183 KKVLGEQGQH--MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWF 242
Query: 244 GVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKC 303
V+P +AFV+NIG LQ +SNG K+ HRAVTN + R S+ + P D M PAK
Sbjct: 243 AVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKP 302
Query: 304 L---INEANPPRYRSLNFKDFQRNYLPRAADTKAVMQ 333
L ++ P Y+ + ++ + + R D + ++
Sbjct: 303 LWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336
BLAST of Lcy05g007860 vs. TAIR 10
Match:
AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 231.5 bits (589), Expect = 1.0e-60
Identity = 129/327 (39.45%), Postives = 191/327 (58.41%), Query Frame = 0
Query: 13 VETVPESYVYPPEKRPG-NVVVPMEKAIPVVDLTTHD-----RALLIRKIFDASKEFGFF 72
V+ VP +YV P RP + V +IP++DL HD RA +I + A GFF
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74
Query: 73 QVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCWR 132
Q+ NHGV + +++ M +EF S +E+ K S D K+ R+ TS N KE+ WR
Sbjct: 75 QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134
Query: 133 DALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLGGE 192
D L +C+P++ +++ WP P +REV Y ++R LVL +LE ISE LGL + +
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194
Query: 193 KSEN-PVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD-VNGLQVLKDGQWIGVHPID 252
++ + +N+YP CP P LT GL H D +LIT+L QD V+GLQV KDG+WI V+P+
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254
Query: 253 NAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA- 312
N F+VN+G +QVISN K K+ HRAV N+ + R S+ P ++ + PA+ LINE
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314
Query: 313 -NPPRYRSLNFKDFQRNYLPRAADTKA 330
+P YR+ + ++ + A DT++
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338
BLAST of Lcy05g007860 vs. TAIR 10
Match:
AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 222.2 bits (565), Expect = 6.1e-58
Identity = 123/334 (36.83%), Postives = 191/334 (57.19%), Query Frame = 0
Query: 7 VSNWEGVETVPESYVYPPEKRPG-NVVVPMEKAIPVVDLTT---HDRALLIRKIFDASKE 66
VS++ +P +YV P RP + V +IP++DL +RA++++++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70
Query: 67 FGFFQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQT 126
+GFFQ+ NHGV + + + +EF +E+ K S DP K+ R+ TS N ++
Sbjct: 71 YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130
Query: 127 HCWRDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGY 186
WRD L +C P++ ++ WP +P +REV Y ++R LVL +LE ISE LGL +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190
Query: 187 LG---GEKSENPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQD-VNGLQVLKDGQWI 246
+ G+ +++ + N+YPPCP P LT GL H DP++IT+L QD V+GLQV KD +W+
Sbjct: 191 ISNILGKHAQH--MAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 250
Query: 247 GVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKC 306
V PI N F+VNIG +QVISN K K+ HRAV N + R S+ +P + + PA
Sbjct: 251 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 310
Query: 307 LINEANP-PRYRSLNFKDFQRNYLPRAADTKAVM 332
L+NE + YR+ F ++ + R+ T + +
Sbjct: 311 LVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341
BLAST of Lcy05g007860 vs. TAIR 10
Match:
AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 208.4 bits (529), Expect = 9.2e-54
Identity = 122/318 (38.36%), Postives = 175/318 (55.03%), Query Frame = 0
Query: 12 GVETVPESYVYPPEKRP--GNVVVPMEKAIPVVDLT-THD---RALLIRKIFDASKEFGF 71
GV VP+ YV PP +RP G+ + E +PV+DL+ H R+L I +I A KEFGF
Sbjct: 23 GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82
Query: 72 FQVINHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCW 131
FQVINHG+ V+ + + +F + EK S + ++ R Y +S N+ ++ H W
Sbjct: 83 FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142
Query: 132 RDALIFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLGG 191
RD + HPL K++ WP NPP Y++ VG Y A L +++E ISE LGL + YL
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202
Query: 192 EKSE-NPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLK-DGQWIGVHPI 251
E E + V+ VN YP CP P + LG+ H D S +TIL Q GLQ++ + W+ V I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262
Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
+ A +V +G ++V+SNG K+ HR N ++ R S L + PA L+N
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322
Query: 312 NPPRYRSLNFKDFQRNYL 322
N P Y +F DF NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338
BLAST of Lcy05g007860 vs. TAIR 10
Match:
AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 192.2 bits (487), Expect = 6.8e-49
Identity = 115/309 (37.22%), Postives = 161/309 (52.10%), Query Frame = 0
Query: 16 VPESYVYPPEKRP--GNVVVPMEKAIPVVDLT-THD---RALLIRKIFDASKEFGFFQVI 75
VP Y+ PP +RP G + +PV+DL+ HD R +I +I A K FGFFQVI
Sbjct: 27 VPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGFFQVI 86
Query: 76 NHGVSRKVLEETMKIFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYFKEQTHCWRDAL 135
NHG+S V+++ F + EK S + + R Y +S N+ ++ H WRD +
Sbjct: 87 NHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHYWRDFI 146
Query: 136 IFNCHPLDKYLHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLGLGRGYLGGEKSE 195
HPL +++ WP NPP Y+E VG Y A L +++E ISE LGL + YL E E
Sbjct: 147 KHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQEEIEE 206
Query: 196 -NPVLLVNHYPPCPNPSLTLGLAQHCDPSLITILFQDVNGLQVLK-DGQWIGVHPIDNAF 255
+ V+ VN YP CP P + LG+ H D +TIL Q GLQ+ + W+ V I+ A
Sbjct: 207 GSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPYIEGAL 266
Query: 256 VVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPR 315
+V +G ++V+SNG K+ HR N R S L + PA L+NE P
Sbjct: 267 IVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNENKPAA 326
Query: 316 YRSLNFKDF 317
Y +F DF
Sbjct: 327 YGEFSFNDF 334
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P24397 | 1.2e-69 | 42.51 | Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1 | [more] |
| Q9FLV0 | 1.5e-69 | 41.84 | Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... | [more] |
| Q8W2X5 | 7.5e-69 | 41.36 | Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... | [more] |
| Q9ZSA7 | 1.4e-59 | 39.45 | Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1 | [more] |
| Q40062 | 1.3e-57 | 40.06 | 2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1H5H6 | 8.4e-180 | 89.25 | hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=... | [more] |
| A0A6J1KW36 | 7.1e-179 | 88.50 | hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ... | [more] |
| A0A0A0K5H3 | 5.1e-177 | 86.98 | Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... | [more] |
| A0A1S3BR20 | 4.3e-176 | 87.28 | hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1 | [more] |
| A0A6J1CP90 | 1.3e-169 | 82.06 | hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_023514953.1 | 1.7e-179 | 88.50 | hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG6593078.1 | 1.7e-179 | 89.25 | Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022959666.1 | 1.7e-179 | 89.25 | hyoscyamine 6-dioxygenase-like [Cucurbita moschata] | [more] |
| KAG7025486.1 | 3.9e-179 | 89.25 | Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| XP_023004374.1 | 1.5e-178 | 88.50 | hyoscyamine 6-dioxygenase-like [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G24530.1 | 1.1e-70 | 41.84 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10490.1 | 1.0e-60 | 39.45 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10500.1 | 6.1e-58 | 36.83 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT2G44800.1 | 9.2e-54 | 38.36 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT3G60290.1 | 6.8e-49 | 37.22 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
