Lcy05g006930 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g006930 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 951.0 bits (2457), Expect = 9.1e-276
Identity = 475/871 (54.54%), Postives = 621/871 (71.30%), Query Frame = 0

Query: 14  CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKV--GFIKYSHRNLCKM 73
           C  N G+  ++K   +   T   +KIK  S  +   R   +G  V      Y    +C  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQFSKICAS 72

Query: 74  YASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGSGFAVYIEMSSL 133
             S  +    S +R     ++ +  KV +Y FRT+ G LV V V K    +++ + +SSL
Sbjct: 73  KTSIELREALSSRR----AEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSL 132

Query: 134 QLASVDGIPM-LSWGVYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFAVELEFD 193
           +L+  D   + + WGVYRSDS+   P +F++S  +    TTET FV +S  +  + LEFD
Sbjct: 133 ELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFD 192

Query: 194 AKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINF 253
            K  PFYLSF LK   G D    E+ +HR T+FCIPVGF  G+P PLGLS    D S NF
Sbjct: 193 GKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNF 252

Query: 254 SIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVSYGYRCKGS 313
           S FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++W+AS +   +FV YGYRCK +
Sbjct: 253 SFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKET 312

Query: 314 KFHENKDKVEVSRVLLDPYAKILTASIPKTCGQGLGQICKVPAFDWDGEARPNLPMEKLF 373
              +    VE   ++LDPYA ++  S+ +   + LG + K P+FDW  +  PN+P+EKL 
Sbjct: 313 AHSKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLL 372

Query: 374 VYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFP 433
           VYRLNVK FT  +SS+L +++ GTFSG+ +K  HLK LG NAVLLEPIF F E++GPYFP
Sbjct: 373 VYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFP 432

Query: 434 SHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTHSAEDGALKGIDDSSY 493
            HFFSP +IYGPS S  SA+NS+K MVK+LH+ GIEV+LEVVFTH+A+ GAL+GIDDSSY
Sbjct: 433 FHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSY 492

Query: 494 YFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLS 553
           Y+  R  +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LS
Sbjct: 493 YYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLS 552

Query: 554 RPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE 613
           RPPLVEAIAFDPLL++TK+IAD WDP E +  E R PHWKRWAE+N+++C +VR+F +G 
Sbjct: 553 RPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR 612

Query: 614 GLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEE 673
           G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELSWNCGEE
Sbjct: 613 GVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEE 672

Query: 674 GPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLK 733
           G TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     RK FDWN+L 
Sbjct: 673 GATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLA 732

Query: 734 TGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVML 793
           + FGTQ TQFI+F++S RA+R D+FQ R+FLK ENI WY  +Q+ P+WEDP+ KFLA+ +
Sbjct: 733 SAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEI 792

Query: 794 R--ADKEENGSIVENPEPRS-DMFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGF 853
           +  +++EE  S+ E  EP+S D+F+ FNASD  ESV LP   +G+ W RL+DTAL FPGF
Sbjct: 793 KSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGF 852

Query: 854 FSSDGEPV----PKTGSVTYEIRAHSCALFE 872
           FS +GE V    P    V YE++ +SC LFE
Sbjct: 853 FSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Lcy05g006930 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 6.0e-219
Identity = 375/722 (51.94%), Postives = 499/722 (69.11%), Query Frame = 0

Query: 176 ASEGKFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPL 235
           A     A EL FD    PFYLSFLL      D+  +EIR+HRKT+F +PVG G G P+PL
Sbjct: 99  AGGASLAAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPL 158

Query: 236 GLSISGDGSINFSIFSSTAESVVLCLYDDST----SEKPVLELDLDPYINRSGNIWYASF 295
           G+SISGDG++NF+++S  A +V L LY  +      ++P LE+DLDPYI+R+GN+W+ S 
Sbjct: 159 GMSISGDGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSL 218

Query: 296 EGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKIL--TASIPKTCGQGL------- 355
                +VSY + C G           + R LLDPYAK++    S      +G+       
Sbjct: 219 ASVDGYVSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRC 278

Query: 356 -GQICKVPAFDWDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLH 415
              +   P+++W  +  P LP+EKL VYR NV  FT D+SS L  D  GTF+GL+ K  H
Sbjct: 279 FASLAIAPSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEH 338

Query: 416 LKNLGVNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANG 475
            ++LGVNA+LLEP+FPF + +GPYFP HFFSP N+Y   G S+SAI S+K+MV+ +H NG
Sbjct: 339 FRSLGVNAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNG 398

Query: 476 IEVILEVVFTHSAEDG------ALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLD 535
           IEV+LEVVFTH+AE        +++GID+SSYY AN +A  +  S L+CN+P+ Q+L+LD
Sbjct: 399 IEVLLEVVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILD 458

Query: 536 SLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQE 595
           SLR+WV +FH+DGFCFINA  L+RG  GE LSRPPL+EAI FDP+LS TKIIAD W P +
Sbjct: 459 SLRHWVLDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLD 518

Query: 596 SVTNETRLPHWKRWAEVNSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFN 655
               +   PHWKRWAEVN++F  DVR F K E L+S LATRLCGSGD+FS  RGPAFSFN
Sbjct: 519 ISNVQFPFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFN 578

Query: 656 FIARNAGLPLVDLVSFSSSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLG 715
            ++RN+GL LVDLVSFS+  L SE SWNCGEEGP+ NS VL+ RL+QIRNF+F+LFVSLG
Sbjct: 579 HVSRNSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLG 638

Query: 716 VPVLNMGDECGQSSGGSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSR 775
           VPVLNMGDECG S+ GSV++ DR   +W  +KT F  + T FI+FL++ R++R D+FQ R
Sbjct: 639 VPVLNMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRR 698

Query: 776 NFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASD 835
            FLK ENI WYG++   P W+DP+  FL + + A+ +E  +       R D+++ FNA++
Sbjct: 699 EFLKLENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANE 758

Query: 836 QSESVALPEPSEGTSWFRLIDTALSFPGFFSSDGEPVPK--TGSVTYEIRAHSCALFEAK 876
           +S S ALP  +EG+ W RL+DT+L+FPGFF+++  P  +   G  +Y + AH+C LFE+K
Sbjct: 759 ESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESK 798

BLAST of Lcy05g006930 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.3e-101
Identity = 256/715 (35.80%), Postives = 364/715 (50.91%), Query Frame = 0

Query: 223 IPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLD 282
           +P  +  G   PLG+S   +G +NF++FS  A SV+LCL       +      V+E  LD
Sbjct: 96  MPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155

Query: 283 PYINRSGNIWYASFEG-ASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTAS--- 342
              N++G+IW+   EG  ++ V YGYR  G +  +   + + S VLLDPYAK+++     
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYF 215

Query: 343 --IPKTCGQGLGQI-CKVPAFDWDGEAR-PNLPMEKLFVYRLNVKRFTVDKSSQLSTDIV 402
               +   Q  G        FDW  + R PNLP   L +Y +NV+ FT D+SS L +   
Sbjct: 216 GVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSR 275

Query: 403 GTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFPS--------------HFFSPTN 462
           G++ GL  K  HL  LGVNAV L P+F +DE E   +P+              +FF+P +
Sbjct: 276 GSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAPMS 335

Query: 463 IYGPSGSS-ISAINSVKEMVKELHANGIEVILEVVFTHS--AEDG-----ALKGIDDSSY 522
            Y  +G   ++A   +K+MVKELH  GIEVIL+VV+ H+  A+D      + +GID+  Y
Sbjct: 336 RYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVY 395

Query: 523 YFANRVANVE------GKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGH 582
           Y  +   N E        + L+CN+P+V++L+LDSLR+WV E+HIDGF F  AS L RG 
Sbjct: 396 YMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCRGP 455

Query: 583 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVR 642
            G  L  PPL++ IA D +LS+ KIIA+ WD    +    R P+W RWAE N K+ +D+R
Sbjct: 456 DGCPLDAPPLIKEIAKDAVLSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGKYRDDLR 515

Query: 643 SFFKGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS------- 702
            F KG+ G+    ATR+ GS D++  + R P    NF+  + G  L DLVS++       
Sbjct: 516 RFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDAN 575

Query: 703 ----SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-- 762
                       SWNCG EG TN+  VL  R +Q++NF   L +S G P++ MGDE G  
Sbjct: 576 GEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHT 635

Query: 763 -----QSSGGSVAFNDRKAFDWNVL---KTGFGTQTTQFITFLSSFRAKRFDLFQSRNFL 822
                 S G     N+   F W  L   + G     ++ I F  S    R D F ++N  
Sbjct: 636 RYGNNNSYGHDTCINN---FQWEQLEQRRDGHFRFFSEMIKFRHSNPILRRDRFLNKN-- 695

Query: 823 KGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSE 874
              ++ W+        WE+   KFLA  +  D    G          D+++AFNA D   
Sbjct: 696 ---DVTWH-----EDCWENQESKFLAFTVH-DHNSGG----------DIYLAFNAHDYFV 755

BLAST of Lcy05g006930 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 369.8 bits (948), Expect = 8.7e-101
Identity = 250/703 (35.56%), Postives = 356/703 (50.64%), Query Frame = 0

Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLDPYINRSG 289
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 290 NIWYASFEGAS-NFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTA-SIPKTCGQGL 349
           + W+   E    N V YGYR  G    +   + + S +LLDPYAK++   S      Q  
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKF 207

Query: 350 GQI-----CKVPAFDWDGEAR-PNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLT 409
            Q       +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G  
Sbjct: 208 AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFI 267

Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP-SG 469
           +K  HL++LG+NAV L P+F FDE E                Y   +FF+P + Y    G
Sbjct: 268 EKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEG 327

Query: 470 SSISAINSVKEMVKELHANGIEVILEVVFTHSAE-------DGALKGIDDSSYYFA---N 529
             I A    KEMVK LH+ GIEVIL+VV+ H+ E         + +GID+  YY     N
Sbjct: 328 DPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNN 387

Query: 530 RVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPP 589
           ++ N  G  + L+CN+P+V +L+LDSLR+WV E+H+DGF F  AS L R   G  LS PP
Sbjct: 388 QLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP 447

Query: 590 LVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE-GL 649
           L+ AIA D +LS+ KIIA+ WD    +    + P+W RWAE N  + +DVR F KG+ G+
Sbjct: 448 LIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGM 507

Query: 650 VSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSKLA 709
             S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +    
Sbjct: 508 KGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCN 567

Query: 710 SELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-------QSSG 769
              SWNCG EG T ++ +   R +Q++NF   L +S G P++ MGDE G        S G
Sbjct: 568 DNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYG 627

Query: 770 GSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQ 829
              + N+   F W  L         +F + +  FR     + +  NFL    I W+  N 
Sbjct: 628 HDTSLNN---FQWKELDAK-KQNHFRFFSEVIKFRHSH-HVLKHENFLTQGEITWHEDN- 687

Query: 830 SPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTS 874
               W++   KFLA  L            +     D+++AFNA D      +P+P  G  
Sbjct: 688 ----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQ 747

BLAST of Lcy05g006930 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.3e-93
Identity = 236/711 (33.19%), Postives = 346/711 (48.66%), Query Frame = 0

Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--DDSTSEKPVLELDLDPYINRSGNIW 289
           G P+PLG + + DG +NF+++S+ A +  LCL+  DD  +++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 290 YASFEGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTA----SIPKTCGQGLG 349
           +   EG  + + YGYR  G          +VS V++DPYAK + +     +P   G    
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 350 QICKV-----PAFDWDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKK 409
           Q+  +       FDW G+     P + L +Y ++++ FT  K S  + +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFT--KHSSSNVEHPGTYIGAISK 278

Query: 410 FLHLKNLGVNAVLLEPIFPFDEKE----------GPYFPSHFFSPTNIYGPSG---SSIS 469
             +LK LGVN V L P   F+E E            Y   +FFSP   Y   G       
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338

Query: 470 AINSVKEMVKELHANGIEVILEVVFTHSAEDG------ALKGIDDSSYYF---ANRVANV 529
           AIN  K  V+E H  GIEVI++VVF H+AE        + +GID+S+YY         N 
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398

Query: 530 EG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRG--------------- 589
            G  +  +CN+P+V++ ++D LRYWV E H+DGF F  AS + RG               
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458

Query: 590 ----HHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKF 649
                 G  L+ PPL++ I+ DP+L   K+IA+ WD    +    + PHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWD-AGGLYQVGQFPHWKIWSEWNGKY 518

Query: 650 CNDVRSFFKG-EGLVSSLATRLCGSGDIF-SDGRGPAFSFNFIARNAGLPLVDLVSFSSS 709
            + VR F KG +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++  
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578

Query: 710 KLAS-----------ELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 769
             +S            LSWNCGEEG      V   R +Q+RNF   L VS GVP+  MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638

Query: 770 ECGQSSGG--SVAFNDR--KAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLK 829
           E G + GG  +   +D     F W+  K    +   +F + ++ FR K+ +     +F  
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698

Query: 830 GENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSES 871
            + + W+G     P W + S +F+A   +             E + ++++AFNAS     
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVAFSTK------------DETKGEIYVAFNASHLPAV 758

BLAST of Lcy05g006930 vs. ExPASy TrEMBL
Match: A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 735/882 (83.33%), Postives = 797/882 (90.36%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATF SS V+QP+ V+N GV E+F+LA SDHHTHGH IK+QSGK DE RMLA G S+ GF
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K S  NL K YAS GISVEK+G RFGISGKS E  KVATY+FRTE GDLVNV V KTGS
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF V IE+SS+QLA+++  PMLSWGVYRSDSA++TPN QSSLP++T+GT ETPFVN SEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKHVPFYLSFLLK+P+GVDSGSSEIRSHRKT+FC+PVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGS+NFSIFSS+AESVVLCLY+DSTSEKPV+ELDLD YINRSGNIW+ SFE  SNFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGY CKGSK H+ +D VE+SR+L+DPYAK+LT S  KT G G       LGQICKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNL MEKLF YRLNVKRFTVDKSSQL+TDI GTF+GLTKK LH KNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEKEGPYFPSHFFSPTNIYGPSG SISAINS+KEMVKELHANGIEV+LEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +AEDG L+GIDDSSYYF NRVAN+EGK+AL+CNYP+VQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ES++ ETR PHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           N KFC+DVR FF+GEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           +AF DR+ FDW++LKTGFGTQTTQFITF SSFRA+RFDLFQSRNFLKGENIDWYG N SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDPSCKFLAVMLRADKEENG   ENPEP+SD+FMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKS 875
           RLIDTALSFPGFFSSDGE VP   SVTYE++AHSCALFEAKS
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 888

BLAST of Lcy05g006930 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 734/884 (83.03%), Postives = 796/884 (90.05%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS +IQPFC YN G  ESFKLAVSD+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 1   MATFLSS-LIQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K  HRN+C+ YA S ISV KSGQ+FG  GK+ E  KVATY+FRTEFGDLV  +VGK GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF   IE+SSL+LAS+D   +LSWGVYRS+SAI+TPNFQS LP ET+G TETPFV  SEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKH PFYLSFLLKYP+GVDSGSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIW+ASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILTASIPKT GQG       LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLF+YRLNVK FTVDKSSQL  D  GTFSGLT+K LH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PSG+SIS++NSVKEMVKELHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +A DGAL+GIDDS YYF NRVAN EGKSAL+CN+PIVQQLLLDSLR+WV EFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQESV  ETR PHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF ND++ FF+GEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAF DRK+F+W+ LKT FGTQTTQFITFLSSFR++RFDLFQSRNFLK ENIDW+  NQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           P WEDPSCKFLAVMLRADKEE+GSI ENP+P+SD+FMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           RL+DTALSFPGFFSSDGEPVP T  VTY ++AHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 883

BLAST of Lcy05g006930 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1501.1 bits (3885), Expect = 0.0e+00
Identity = 729/884 (82.47%), Postives = 797/884 (90.16%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
           +K SHRNL K Y +SGI V KSGQR GI GKS E  KVATY+FRTEFGDLVNV+VGKTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
            F V IE+SS+QL S D   +LSW VYRSDSA +T NFQSS P+ T G TETPFV  SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIW+ASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGY+CKG K HEN+D +EVSR+++DPYAKIL  SIPK+  QG       LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLFVYRLNVKRFT+DKSSQL  DI GTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           ++ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTN  +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAFNDR++F+W++LKT FGTQTTQFI FLSSFR++RFDLFQSRNFLKGENIDW+  NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAVMLRADKEEN SI ENP+ RSD+FMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           R++DTAL FPGFFSSDGE VP TGSVTYE++AHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Lcy05g006930 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 728/884 (82.35%), Postives = 796/884 (90.05%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
           +K SHRNL K Y +SGISV KSGQR GI GKS E  KVATY+FRTEFGDLVNV+VGKTGS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
            F V IE+SS+QL S D   +LSW VYRSDSA +T NFQSS P+ T G TETPFV  SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSHRKT+F +PVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIW+ASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGY+CKG K HEN+D +EVSR+++DPYAKIL  SIPK+  QG       LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLFVYRLNVKRFT+DKSSQL  DI GTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           ++ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTN  +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAFNDR++F+W++LKT FGTQTTQFI FLSSFR++RFDLFQSRNFLKGENIDW+  NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAVMLRADKEEN SI ENP+ RSD+FMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           R++DTAL FPGFFSSDGE VP TGSVTYE++AHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Lcy05g006930 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 720/884 (81.45%), Postives = 795/884 (89.93%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS VIQPFCVYNCGV ES KLA SDH T+G K KYQ GKMDEARMLA G +KVG 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
           +K SHRNL K YA SGISV KSGQR GI GKS E  +VATY+FRTEFGDLVNV+VGK GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
            F V IE+ S+QL S+D   +LSWGVYRSDSA++TPNF+SS P+ET G TETPFV  SEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEIRSH+KT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGS+NFSIFSS+AES+VLCLY+DSTSEKP+LELDLDPYINRSGNIW+ASFEGAS FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGY+CKGSK HEN+D +EVSR+++DPYAKIL  SIPK+ GQG       LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE  PNLPMEKLFVYRLNV+RFT+DKSSQL  DI GTFSGLTKK LH KNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPSG+SISAINS+KEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           ++ +GAL+GIDDSSYYF NRVAN+E KSAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NAS LLRGHHGELLSRPP VEAIAFDPLLSKTK++ADFWDPQE  + ETR PHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+N +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAFND+++F+W++LKT FGTQTTQFI FLSSFR++RFDLFQ+RNFLKGENIDW+  NQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAVMLRADKEEN SI ENP+ RS++FM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           R++DTAL FPGFFSSDGE VP TGSVTYEI+AHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884

BLAST of Lcy05g006930 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1537.7 bits (3980), Expect = 0.0e+00
Identity = 747/883 (84.60%), Postives = 806/883 (91.28%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS VIQPFCVYN GV ESFKLAV DHHT+G KIKY SGKMDEARMLA G S+VG 
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K SHRNL K+YA+SGI V KSGQRFG  GKS EH KVATY+FRTEFGDLVNV+VGKTGS
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF V +E+SS+QLAS++   +LSWGVYRSDSAI+TPNFQSSLP+ET+G TETPFV  S G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKH PFYLSFLLKYP+GVDSGSSEIRSHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLD YINRSG+IW+ASFE ASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG------LGQICKVPAFDW 360
           YGYRCKGSKFHEN+D VEV+R+++DPYAKILTAS+ KT GQG      LGQICKVPAFDW
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLPKFLGQICKVPAFDW 360

Query: 361 DGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLLE 420
           DGE  PNLPMEKLF YRLNVKRFTVDKSSQL  DI GTFSGLTKK LH KNLGVNAVLLE
Sbjct: 361 DGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLE 420

Query: 421 PIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTHS 480
           PIF FDEKEG YFP HFFSP N YGPSG SISAINS+K MVK+LHANG+EVILEVVFTH+
Sbjct: 421 PIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTHT 480

Query: 481 AEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFIN 540
           A DGAL+GIDDSSYYF NRVAN+EGKSAL+CNYPIVQQLLLDSLRYWV EFHIDGFCFIN
Sbjct: 481 AMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFIN 540

Query: 541 ASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVN 600
           ASSLLRGHHGE LSR P VEAIAFDPLLSKTK++ADFWDPQE V+ ET  PHWKRWAEVN
Sbjct: 541 ASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEVN 600

Query: 601 SKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSS 660
           SKFCNDVR FF+GEGL+SSLATRLCGSGD+FS+GRGPAFSFNFI RN GLPLVDLVSFS+
Sbjct: 601 SKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFSN 660

Query: 661 SKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720
           SKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGSV 720

Query: 721 AFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSPP 780
           AFNDR++F+W++LKT FGTQTTQFITFLSSFR++RFDLFQSRNFLKGENIDW+  NQSPP
Sbjct: 721 AFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPP 780

Query: 781 QWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWFR 840
           QWEDPSCKFLAVMLRADKEENGSI EN +PRSD+FMAFNASDQSE+VALPEPSEGTSWFR
Sbjct: 781 QWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWFR 840

Query: 841 LIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           L+DTALSFPGFFSSDGE VP TGSVTYE++AHSCALFEAKS N
Sbjct: 841 LVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFN 883

BLAST of Lcy05g006930 vs. NCBI nr
Match: XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 735/882 (83.33%), Postives = 797/882 (90.36%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATF SS V+QP+ V+N GV E+F+LA SDHHTHGH IK+QSGK DE RMLA G S+ GF
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K S  NL K YAS GISVEK+G RFGISGKS E  KVATY+FRTE GDLVNV V KTGS
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF V IE+SS+QLA+++  PMLSWGVYRSDSA++TPN QSSLP++T+GT ETPFVN SEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKHVPFYLSFLLK+P+GVDSGSSEIRSHRKT+FC+PVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGS+NFSIFSS+AESVVLCLY+DSTSEKPV+ELDLD YINRSGNIW+ SFE  SNFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGY CKGSK H+ +D VE+SR+L+DPYAK+LT S  KT G G       LGQICKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNL MEKLF YRLNVKRFTVDKSSQL+TDI GTF+GLTKK LH KNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEKEGPYFPSHFFSPTNIYGPSG SISAINS+KEMVKELHANGIEV+LEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +AEDG L+GIDDSSYYF NRVAN+EGK+AL+CNYP+VQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ES++ ETR PHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           N KFC+DVR FF+GEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           +AF DR+ FDW++LKTGFGTQTTQFITF SSFRA+RFDLFQSRNFLKGENIDWYG N SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDPSCKFLAVMLRADKEENG   ENPEP+SD+FMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKS 875
           RLIDTALSFPGFFSSDGE VP   SVTYE++AHSCALFEAKS
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 888

BLAST of Lcy05g006930 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1505.0 bits (3895), Expect = 0.0e+00
Identity = 732/884 (82.81%), Postives = 797/884 (90.16%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS +IQPFC YN G  ESFKLAV+D+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 24  MATFLSS-LIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K  HRN+CK YA SGISV KSGQ+FG  GK+ E  KVATY+FRTEFGDLV  +VGK GS
Sbjct: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF V IE+SSL+LAS+D   +LSWGVYRS+SAI+TPNFQS LP ET+G TETPFV  SEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKH PFYLSFLLKYP+GVDSGSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIW+ SFEGASNFVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILTAS PKT GQG       LGQIC VPAF 
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLF+YRLNVK FTVDKSSQL  DI GTFSGLT+K LH K LGVNAVLL
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PSG+SIS++NSVKEMVKELHANG+EVILEVVFTH
Sbjct: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +A DGAL+GIDDS YYF NRVAN EGKSAL+CN+PIVQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIA+FWDPQE V  ETR PHWKRWAE+
Sbjct: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF ND++ FF+GEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAF DRK+F+W+ LKT FGTQTT+FI F SSFR++RFDLFQSR+FLKGENIDW+  NQSP
Sbjct: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           P+WEDPSCKFLAVMLRADKEE+GSI ENP+P+SD+FMAFNASDQS SVALPEPSEGT+WF
Sbjct: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           RL+DTALSFPGFFSSDGEPVP T  VTYE++AHSC LFEAKS N
Sbjct: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFN 906

BLAST of Lcy05g006930 vs. NCBI nr
Match: XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 734/884 (83.03%), Postives = 796/884 (90.05%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS +IQPFC YN G  ESFKLAVSD+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 1   MATFLSS-LIQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K  HRN+C+ YA S ISV KSGQ+FG  GK+ E  KVATY+FRTEFGDLV  +VGK GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF   IE+SSL+LAS+D   +LSWGVYRS+SAI+TPNFQS LP ET+G TETPFV  SEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKH PFYLSFLLKYP+GVDSGSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIW+ASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILTASIPKT GQG       LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLF+YRLNVK FTVDKSSQL  D  GTFSGLT+K LH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PSG+SIS++NSVKEMVKELHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +A DGAL+GIDDS YYF NRVAN EGKSAL+CN+PIVQQLLLDSLR+WV EFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQESV  ETR PHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF ND++ FF+GEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAF DRK+F+W+ LKT FGTQTTQFITFLSSFR++RFDLFQSRNFLK ENIDW+  NQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           P WEDPSCKFLAVMLRADKEE+GSI ENP+P+SD+FMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           RL+DTALSFPGFFSSDGEPVP T  VTY ++AHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 883

BLAST of Lcy05g006930 vs. NCBI nr
Match: XP_023548355.1 (isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548356.1 isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 731/884 (82.69%), Postives = 794/884 (89.82%), Query Frame = 0

Query: 1   MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
           MATFLSS +IQPFC YN G  ESFKLAV+D+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 1   MATFLSS-LIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  IKYSHRNLCKMYASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGS 120
            K  HRN+C+ YA SGISV KSGQ+FG  GK+ E  KVATY+FRTEFGDLV  +VGK GS
Sbjct: 61  TKSLHRNVCRAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 GFAVYIEMSSLQLASVDGIPMLSWGVYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
           GF V IE+SSL+LAS+D   +LSWGVYRS+SAI+TPNFQS LP ET+G TE PFV  SEG
Sbjct: 121 GFMVNIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATEAPFVRTSEG 180

Query: 181 KFAVELEFDAKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
           KFAVELEFDAKH PFYLSFLLKYP+GVDSGSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIW+ASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTASIPKTCGQG-------LGQICKVPAFD 360
           YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILTASIPKT GQG       LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLL 420
           WDGE RPNLPMEKLF+YRLNVK FTVDKSSQL  DI GTFSGLT+K LH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PSG+SIS++NSVKEMVK LHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKVLHANGVEVILEVVFTH 480

Query: 481 SAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
           +A DGAL+GIDDS YYF NRV N EGKSAL+CN+PIVQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVTNSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
           NASSLL+GHHGELLSRPP  EAIAFDPLLSKTKIIADFWDPQESV  E R PHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFAEAIAFDPLLSKTKIIADFWDPQESVPKEIRFPHWKRWAEL 600

Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF ND++ FF+GEGLV SLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVRSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSP 780
           VAF DRK+F+W+ LKT FGTQTTQFITFLSSFR++RFDLFQSRNFLK ENIDW+  NQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTSWF 840
           P WEDPSCKFLAVMLRADKEE+GSI ENP+P+SD+FMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTTWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEIRAHSCALFEAKSSN 877
           RL+DTALSFPGFFSSDGEPVP T  +TYE++AHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPITYEMQAHSCTLFEAKSFN 883

BLAST of Lcy05g006930 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 951.0 bits (2457), Expect = 6.5e-277
Identity = 475/871 (54.54%), Postives = 621/871 (71.30%), Query Frame = 0

Query: 14  CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKV--GFIKYSHRNLCKM 73
           C  N G+  ++K   +   T   +KIK  S  +   R   +G  V      Y    +C  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQFSKICAS 72

Query: 74  YASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGSGFAVYIEMSSL 133
             S  +    S +R     ++ +  KV +Y FRT+ G LV V V K    +++ + +SSL
Sbjct: 73  KTSIELREALSSRR----AEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSL 132

Query: 134 QLASVDGIPM-LSWGVYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFAVELEFD 193
           +L+  D   + + WGVYRSDS+   P +F++S  +    TTET FV +S  +  + LEFD
Sbjct: 133 ELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFD 192

Query: 194 AKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINF 253
            K  PFYLSF LK   G D    E+ +HR T+FCIPVGF  G+P PLGLS    D S NF
Sbjct: 193 GKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNF 252

Query: 254 SIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVSYGYRCKGS 313
           S FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++W+AS +   +FV YGYRCK +
Sbjct: 253 SFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKET 312

Query: 314 KFHENKDKVEVSRVLLDPYAKILTASIPKTCGQGLGQICKVPAFDWDGEARPNLPMEKLF 373
              +    VE   ++LDPYA ++  S+ +   + LG + K P+FDW  +  PN+P+EKL 
Sbjct: 313 AHSKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLL 372

Query: 374 VYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFP 433
           VYRLNVK FT  +SS+L +++ GTFSG+ +K  HLK LG NAVLLEPIF F E++GPYFP
Sbjct: 373 VYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFP 432

Query: 434 SHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTHSAEDGALKGIDDSSY 493
            HFFSP +IYGPS S  SA+NS+K MVK+LH+ GIEV+LEVVFTH+A+ GAL+GIDDSSY
Sbjct: 433 FHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSY 492

Query: 494 YFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLS 553
           Y+  R  +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LS
Sbjct: 493 YYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLS 552

Query: 554 RPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE 613
           RPPLVEAIAFDPLL++TK+IAD WDP E +  E R PHWKRWAE+N+++C +VR+F +G 
Sbjct: 553 RPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR 612

Query: 614 GLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEE 673
           G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELSWNCGEE
Sbjct: 613 GVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEE 672

Query: 674 GPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLK 733
           G TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     RK FDWN+L 
Sbjct: 673 GATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLA 732

Query: 734 TGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVML 793
           + FGTQ TQFI+F++S RA+R D+FQ R+FLK ENI WY  +Q+ P+WEDP+ KFLA+ +
Sbjct: 733 SAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEI 792

Query: 794 R--ADKEENGSIVENPEPRS-DMFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGF 853
           +  +++EE  S+ E  EP+S D+F+ FNASD  ESV LP   +G+ W RL+DTAL FPGF
Sbjct: 793 KSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGF 852

Query: 854 FSSDGEPV----PKTGSVTYEIRAHSCALFE 872
           FS +GE V    P    V YE++ +SC LFE
Sbjct: 853 FSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Lcy05g006930 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 951.0 bits (2457), Expect = 6.5e-277
Identity = 475/871 (54.54%), Postives = 621/871 (71.30%), Query Frame = 0

Query: 14  CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKV--GFIKYSHRNLCKM 73
           C  N G+  ++K   +   T   +KIK  S  +   R   +G  V      Y    +C  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQFSKICAS 72

Query: 74  YASSGISVEKSGQRFGISGKSTEHTKVATYMFRTEFGDLVNVYVGKTGSGFAVYIEMSSL 133
             S  +    S +R     ++ +  KV +Y FRT+ G LV V V K    +++ + +SSL
Sbjct: 73  KTSIELREALSSRR----AEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSL 132

Query: 134 QLASVDGIPM-LSWGVYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFAVELEFD 193
           +L+  D   + + WGVYRSDS+   P +F++S  +    TTET FV +S  +  + LEFD
Sbjct: 133 ELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFD 192

Query: 194 AKHVPFYLSFLLKYPLGVDSGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINF 253
            K  PFYLSF LK   G D    E+ +HR T+FCIPVGF  G+P PLGLS    D S NF
Sbjct: 193 GKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNF 252

Query: 254 SIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWYASFEGASNFVSYGYRCKGS 313
           S FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++W+AS +   +FV YGYRCK +
Sbjct: 253 SFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKET 312

Query: 314 KFHENKDKVEVSRVLLDPYAKILTASIPKTCGQGLGQICKVPAFDWDGEARPNLPMEKLF 373
              +    VE   ++LDPYA ++  S+ +   + LG + K P+FDW  +  PN+P+EKL 
Sbjct: 313 AHSKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLL 372

Query: 374 VYRLNVKRFTVDKSSQLSTDIVGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFP 433
           VYRLNVK FT  +SS+L +++ GTFSG+ +K  HLK LG NAVLLEPIF F E++GPYFP
Sbjct: 373 VYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFP 432

Query: 434 SHFFSPTNIYGPSGSSISAINSVKEMVKELHANGIEVILEVVFTHSAEDGALKGIDDSSY 493
            HFFSP +IYGPS S  SA+NS+K MVK+LH+ GIEV+LEVVFTH+A+ GAL+GIDDSSY
Sbjct: 433 FHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSY 492

Query: 494 YFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLS 553
           Y+  R  +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LS
Sbjct: 493 YYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLS 552

Query: 554 RPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE 613
           RPPLVEAIAFDPLL++TK+IAD WDP E +  E R PHWKRWAE+N+++C +VR+F +G 
Sbjct: 553 RPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR 612

Query: 614 GLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEE 673
           G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELSWNCGEE
Sbjct: 613 GVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEE 672

Query: 674 GPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLK 733
           G TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     RK FDWN+L 
Sbjct: 673 GATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLA 732

Query: 734 TGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVML 793
           + FGTQ TQFI+F++S RA+R D+FQ R+FLK ENI WY  +Q+ P+WEDP+ KFLA+ +
Sbjct: 733 SAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEI 792

Query: 794 R--ADKEENGSIVENPEPRS-DMFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGF 853
           +  +++EE  S+ E  EP+S D+F+ FNASD  ESV LP   +G+ W RL+DTAL FPGF
Sbjct: 793 KSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGF 852

Query: 854 FSSDGEPV----PKTGSVTYEIRAHSCALFE 872
           FS +GE V    P    V YE++ +SC LFE
Sbjct: 853 FSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Lcy05g006930 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 369.8 bits (948), Expect = 6.2e-102
Identity = 250/703 (35.56%), Postives = 356/703 (50.64%), Query Frame = 0

Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLDPYINRSG 289
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 290 NIWYASFEGAS-NFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTA-SIPKTCGQGL 349
           + W+   E    N V YGYR  G    +   + + S +LLDPYAK++   S      Q  
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKF 207

Query: 350 GQI-----CKVPAFDWDGEAR-PNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLT 409
            Q       +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G  
Sbjct: 208 AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFI 267

Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP-SG 469
           +K  HL++LG+NAV L P+F FDE E                Y   +FF+P + Y    G
Sbjct: 268 EKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEG 327

Query: 470 SSISAINSVKEMVKELHANGIEVILEVVFTHSAE-------DGALKGIDDSSYYFA---N 529
             I A    KEMVK LH+ GIEVIL+VV+ H+ E         + +GID+  YY     N
Sbjct: 328 DPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNN 387

Query: 530 RVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPP 589
           ++ N  G  + L+CN+P+V +L+LDSLR+WV E+H+DGF F  AS L R   G  LS PP
Sbjct: 388 QLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP 447

Query: 590 LVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE-GL 649
           L+ AIA D +LS+ KIIA+ WD    +    + P+W RWAE N  + +DVR F KG+ G+
Sbjct: 448 LIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGM 507

Query: 650 VSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSKLA 709
             S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +    
Sbjct: 508 KGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCN 567

Query: 710 SELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-------QSSG 769
              SWNCG EG T ++ +   R +Q++NF   L +S G P++ MGDE G        S G
Sbjct: 568 DNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYG 627

Query: 770 GSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQSRNFLKGENIDWYGTNQ 829
              + N+   F W  L         +F + +  FR     + +  NFL    I W+  N 
Sbjct: 628 HDTSLNN---FQWKELDAK-KQNHFRFFSEVIKFRHSH-HVLKHENFLTQGEITWHEDN- 687

Query: 830 SPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNASDQSESVALPEPSEGTS 874
               W++   KFLA  L            +     D+++AFNA D      +P+P  G  
Sbjct: 688 ----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQ 747

BLAST of Lcy05g006930 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 329.7 bits (844), Expect = 7.1e-90
Identity = 228/689 (33.09%), Postives = 335/689 (48.62%), Query Frame = 0

Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YDDSTSEKPVLELDLDPYINRSGNIW 289
           G PSP G ++  DG +NFS++S+ + S  +CL    D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 290 YASFEGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTA-------SIPKTCGQ 349
           +    G    + YGYR  G    E     + S +LLDPYAK + +            C  
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWP 191

Query: 350 GLGQICKVPA----FDWDGEARPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIVGTFSGLT 409
            +   C VP     FDW+G+    LP + L +Y ++V+ FT  +SS++  +  GT+ G+ 
Sbjct: 192 QMA--CMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVA 251

Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE---------------GPYFPSHFFSPTNIYGPSG 469
           +K  HLK LG+N + L P   F+E E                 Y    FFSP   Y  + 
Sbjct: 252 EKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASAS 311

Query: 470 SSI---SAINSVKEMVKELHANGIEVILEVVFTHSAEDG------ALKGIDDSSYYF--- 529
           S+     AIN  K +VKE H  GIEVI++VV  H+AE        + +G+D+S YY    
Sbjct: 312 SNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAP 371

Query: 530 ANRVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGH------- 589
                N  G  +  +CN+P+V+Q +LD LRYWV E H+DGF F   S + R         
Sbjct: 372 KGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAAN 431

Query: 590 ------HGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRW 649
                  G+LL      S PP+++ I+ DP+L   K+IA+ WD    +      PHW  W
Sbjct: 432 VYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWD-AGGLYQVGMFPHWGIW 491

Query: 650 AEVNSKFCNDVRSFFKG-EGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDL 709
           +E N KF + VR F KG +G   + A  LCGS +++  GR P  S NFI  + G  L DL
Sbjct: 492 SEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADL 551

Query: 710 VSFSS-SKLAS----------ELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVP 769
           V++++ + LA+            SWNCGEEG   +  V   R +Q+RNF   L VS GVP
Sbjct: 552 VTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVP 611

Query: 770 VLNMGDECGQSSGGS----VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRAKRFDLFQ 829
           ++ MGDE G + GG+       N    F W+  K    +   +F   L  FR    +   
Sbjct: 612 MIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFR-DECESLG 671

Query: 830 SRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIVENPEPRSDMFMAFNA 843
             +F   + + W+G     P W + S +F+A           S+V++   + ++++AFN 
Sbjct: 672 LNDFPTAKRLQWHGLAPEIPNWSETS-RFVAF----------SLVDS--VKKEIYVAFNT 731