Lcy05g006840 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g006840 vs. ExPASy Swiss-Prot
Match: F4IN58 (Piezo-type mechanosensitive ion channel homolog OS=Arabidopsis thaliana OX=3702 GN=At2g48060/At2g48040/At2g48050 PE=2 SV=1)

HSP 1 Score: 3275.3 bits (8491), Expect = 0.0e+00
Identity = 1687/2487 (67.83%), Postives = 2000/2487 (80.42%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            M SFL GFLLP LLL AALINWS++S  DL+ FL + +   +IG+ F RR  +LWPI IF
Sbjct: 1    MASFLVGFLLPSLLLAAALINWSVISFLDLIAFLLVHYIAPEIGYRFQRRHWLLWPIFIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            S  V ++QV +LVIW+  G  W   +  W+++IGFM ++SW++P+V+YFL +QLL  LVA
Sbjct: 61   SFAVFLAQVVYLVIWAALGQDWDTPDTGWMRVIGFMILKSWRNPTVMYFLALQLLTSLVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADIY  R G     D+ W HF    + LGSHLRVASCLLLPA+Q+ VGI +PSWVSLPF
Sbjct: 121  LADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRVASCLLLPAVQLAVGICNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSC GLVDWSLTSN  GLFRWWR L +YAGF+I L+Y+YQLP+ +  M++W+A FIGL
Sbjct: 181  FIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNIVLLYLYQLPINFSDMIRWIASFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            F+IS  +E P ICS + L+LFYIMLS V+ DLE+MDFIMS  E+NL E+LLP K+SFFIR
Sbjct: 241  FRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDMDFIMSTSENNLAERLLPPKYSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            E R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL ALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLFALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAF+FLN K+GK                 
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFSFLNTKVGK----------------- 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
                  FGLG+LVAL NLVNNSVFL LS E  RSSN+ S      ETKVL+VATIAWGLR
Sbjct: 421  ------FGLGMLVALGNLVNNSVFLYLSEESSRSSNERSYVEADEETKVLVVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            K SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLSH+++RK+R+SLILL  VHFALLY+L
Sbjct: 481  KCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLSHNINRKIRKSLILLCEVHFALLYIL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            +I LVS+ L +EGS  RE+L QLGLL  +S W+FLEIALLACFC IHNHGFE+LFSFSAI
Sbjct: 541  EIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFLEIALLACFCAIHNHGFEVLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPP+GFSIL+AG NKSVLLSVY++ +++Y  D+ ++ER IASFLS+IG+KFLSMY
Sbjct: 601  VRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYSQDNTTYERHIASFLSAIGQKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAF+TIL++V+ VKPNY+SFG++FLLL+WI GRQL E TKRRLWFPLKAYA +V
Sbjct: 661  RSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLWITGRQLFEETKRRLWFPLKAYAVLV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF S ++WLS  IDL FYLGYNS+A  L N W+SLAVLIVMQLYSYERRQS 
Sbjct: 721  FMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPLLDNVWESLAVLIVMQLYSYERRQSG 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            +    +  LL     GF +RFL WH  KILFAALFYAS+SPIS FG +YLLGLVIC T P
Sbjct: 781  HYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALFYASLSPISVFGFVYLLGLVICTTFP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K+S IPSK FL YTG +++AEYLFQ+WG QA+MFPGQK++ LS++LG RV+EPG WG+E 
Sbjct: 841  KSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFPGQKYAELSFYLGLRVYEPGFWGIES 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVL++AACTLQYNVFRWLE+  G  + KGK+++PCPLFVS ED    +S ++ +N 
Sbjct: 901  GLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKYEEPCPLFVSAEDTTASVSSSNGENP 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
             S D   + +    +   S   F+P  +  +     K   SE  SS+K SFG  WGS KE
Sbjct: 961  SSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLHPKTGGSESGSSRKFSFGHFWGSIKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SH+W++ RI++L+KERFE QK + KIY+KFW+EN+FNL+GLEI MI+LLLASFALLN++S
Sbjct: 1021 SHRWNRRRILALKKERFETQKNLLKIYLKFWIENMFNLYGLEINMIALLLASFALLNAIS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWQPSKAGVHCHD 1140
            + Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +EY+A W +   S+  PS+  VHCHD
Sbjct: 1081 MVYIALLAACVLLRRRVIQKLWPVVVFLFASILAIEYVATWNSFLPSDQAPSETSVHCHD 1140

Query: 1141 CWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMMS 1200
            CW IA LYF+FC  CWLG+ VDD R L SYFVVFML+  KLRADH+S F  SSTY +M S
Sbjct: 1141 CWSIAALYFKFCRECWLGVRVDDPRTLISYFVVFMLACFKLRADHISSFSESSTYHQMKS 1200

Query: 1201 QRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            QRKN+ VWRDLSFETKSMWT+LDYLRLYCY HLLD+VL LILITGTLEYD+LHLGYLAFA
Sbjct: 1201 QRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLLDVVLILILITGTLEYDILHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVF R+RLEILKKKNK+F+FLR YNF LII SLAYQSP VG  + GKCET+ YI+E+IGF
Sbjct: 1261 LVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLAYQSPFVGNFNDGKCETVDYIYEVIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYV RYLEAEQIGAIVREQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVSRYLEAEQIGAIVREQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLRK 1440
             KTEQLQ IR++EE KRQRNLQVEKMKSEMLNL++QLH MNS  D N    SP  E LR+
Sbjct: 1381 RKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLRVQLHRMNS--DSNFGVASPRTEGLRR 1440

Query: 1441 RGTSRIHDD--AMTIDIEGTL-GKVEQILREDSFPHPELQESLASLRTEITTESRMHSME 1500
            R +  +  D  A + +I+G +  K EQ + EDS    E  E   S   E   +S  +S  
Sbjct: 1441 RKSPYLIPDSGAASPEIDGVVHRKEEQPIDEDSQYPFEAHEFPVSTTPE-ALDSPEYSFG 1500

Query: 1501 LPVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNL 1560
                EI EV   +  +S++ +RK+K +G  K NPLISAVQ IGDGVSQVQ IGNQAV+NL
Sbjct: 1501 ASPCEITEVQQDLDVMSMERERKQKSEG--KENPLISAVQLIGDGVSQVQFIGNQAVNNL 1560

Query: 1561 ASFLNVS-QDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIF 1620
             +FLN+S ++ D NEQ++ +D VYD++ESQ+ ++   ERS+SLQSD+SSD  S Q+GRIF
Sbjct: 1561 VNFLNISPENSDTNEQSSVDDEVYDEMESQKRKHTPFERSTSLQSDRSSDGTSFQIGRIF 1620

Query: 1621 RHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIY 1680
            RHIWS+M+SNND+VCYCCFI+ FLWNFSLLSM YLAALF+YALCV+TGP ++FWV+ML+Y
Sbjct: 1621 RHIWSRMQSNNDIVCYCCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMY 1680

Query: 1681 TELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSS 1740
            TE+Y+LLQYLYQIIIQHCGL+ID+ LL ELGFPT RI SSFVVSSLPLFL+Y+FTL+QSS
Sbjct: 1681 TEIYILLQYLYQIIIQHCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSS 1740

Query: 1741 ITAKDGEWTYSNAF--NKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKS 1800
            IT KDG+W  S  F   ++A   ++ L    L++R  ++    R     V+RS+ +YW S
Sbjct: 1741 ITVKDGDWVPSADFTSRRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWIS 1800

Query: 1801 LTQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSR 1860
            LT+GAESPPYF+QV+MDV +WPEDGIQPER+E  +NQLL+++H ERC++ NP  CP+SSR
Sbjct: 1801 LTRGAESPPYFVQVTMDVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSR 1860

Query: 1861 VHVQSIVRSKENTKVALVVLEVVYSSP-SADTCAEWCDSLTPAADVANEIRLAQRNGFVE 1920
            VHVQSI RS E    ALVVLEV Y+SP +  + AEW  SLTPA+DVA EIR AQ +G  E
Sbjct: 1861 VHVQSIERSTETPNEALVVLEVEYASPTNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGE 1920

Query: 1921 NTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQF 1980
             TGFPY ILSVIGGGKR+ DLYAY+FGADLIVFF VAIFYQSVIKN SEF+DVYQLEDQF
Sbjct: 1921 GTGFPYPILSVIGGGKRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQF 1980

Query: 1981 PKEFVFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAG 2040
            P +FV ILM+IFFLIV+DR IYLCSFATGKV++YLF+L+LFTYAVT+YAW I P+ QHA 
Sbjct: 1981 PFDFVIILMVIFFLIVVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAA 2040

Query: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100
             LALR IFLAKA+SLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY GYRLYRALPFLYE
Sbjct: 2041 GLALRIIFLAKAMSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYE 2100

Query: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGIC 2160
            LRCVLDWSCT TSLTMYDWLKLED+NASLYLVKCD VLNR+ HK GEKQT MTKCCNGIC
Sbjct: 2101 LRCVLDWSCTATSLTMYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGIC 2160

Query: 2161 LFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKL 2220
            LFFIL+CVIWAPMLMYSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+IS D +
Sbjct: 2161 LFFILLCVIWAPMLMYSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNI 2220

Query: 2221 NANVVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWI 2280
            +  + L    +L  YNKNDIQLICCQADASVLWLVP  V +RFI SLD + D+ I FTW+
Sbjct: 2221 DLGLDLGSQSFLPTYNKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWV 2280

Query: 2281 LTRERPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRP 2340
            L R+RPKGKE V+Y+R V   D+P +SD+Q VLNGSM+GFR+ N+Y ++FRVTGSGDVR 
Sbjct: 2281 LNRDRPKGKETVKYERSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRS 2340

Query: 2341 LEQEDSFVSADLILNRENHE-WWSFHDIQPI-NVSKCGHFTGPVAFVISEEIPPQGILGD 2400
             E +   VSAD+++N  N + WWSFH+++   N+S C    GPVA ++SEE PPQG LGD
Sbjct: 2341 FEDQTDEVSADILINHANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGD 2400

Query: 2401 TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGEL 2460
            TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICED+YAARAEGEL
Sbjct: 2401 TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDLYAARAEGEL 2459

Query: 2461 GVEEVLYWTLVKIYRSPHMLLEYTKID 2476
            GVEEVLYWTLVKIYRSPHMLLEYTK+D
Sbjct: 2461 GVEEVLYWTLVKIYRSPHMLLEYTKLD 2459

BLAST of Lcy05g006840 vs. ExPASy Swiss-Prot
Match: Q54S52 (Protein PIEZO homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282801 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 9.4e-52
Identity = 155/602 (25.75%), Postives = 274/602 (45.51%), Query Frame = 0

Query: 1933 DLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVFILMIIFFLIVLDR 1992
            D Y  +   D    FF+ IF Q+     S  +  +  ++  P++++ IL+  F +I+LDR
Sbjct: 2456 DYYMPLLFTDFACLFFLVIFPQNFTGIPSSDIAEFLEQNVIPRQYIVILLAQFGVIILDR 2515

Query: 1993 CIYLCSFATGK--------VIFYLFNLVLFTYAVTK-----YAWQIEPSNQHAGELALRA 2052
             IYL      K        V++++F    F   + K     Y W             L  
Sbjct: 2516 IIYLYKSVKAKFVLQIVLTVLYHVFLFFYFPDLIVKPFSFGYTW------------PLVV 2575

Query: 2053 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2112
             +L K + L   A+QI YG P  S    +FL    S  + +GY LY+A+PF+YELR +LD
Sbjct: 2576 FYLMKCIYLYYSALQICYGYPILS--QNRFLMDGYSDFHNIGYALYKAIPFVYELRTLLD 2635

Query: 2113 WSCTTTSLTMYDWLKLEDINASLYLVKCDAV-LNRSQHKQGEKQTVMTKCCNGICLFFIL 2172
            W  T T++  YDWLK ED+ ++++ VKC    + R   ++G KQ    K   G+  F  L
Sbjct: 2636 WIATDTTMLFYDWLKFEDLYSTIFSVKCRLEWIKRQGRQKGHKQPKFEKFVTGVTFFIGL 2695

Query: 2173 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEK----ISWDKLN 2232
            + ++W P+++ SSG P +   P+ +   +V +   +  L + Q    E      + D+ +
Sbjct: 2696 VILLWFPLIILSSGLPGSNIEPVNNIQIEVSVVGWNPFLKINQDLSLESGDGDSTMDQNS 2755

Query: 2233 ANVVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWIL 2292
             N + +   +L+  ++  IQ I     +  +W +    +++ I+ L  N  + I  ++ L
Sbjct: 2756 FNNLKEDYSFLTTDDRQGIQNISINTFSEEIWNLSPPAKAQLINYLLTNTSLQIEVSYTL 2815

Query: 2293 TRERPKGKEVVRYDRLVDIPDQS-DVQKVL------------------------------ 2352
            TR       +V  + +   PDQ  +   +L                              
Sbjct: 2816 TRSGGVNSVIVGSNSVQLKPDQELNFYNILTRVQSNNSNNSNNPNENSSSGSDDNNNNSN 2875

Query: 2353 -NGSMNGFRIKNVYQRYFRVTG-SGD-VRPLEQEDSFVSADLIL----------NRENHE 2412
             N   N F ++ ++ ++ ++ G SG+ + P +   + ++ D +           N+    
Sbjct: 2876 NNIGSNSFIVEGLFYKFIKLPGVSGNPIYPTDNNGNVLALDALFTMNSTNFNNSNQPPQY 2935

Query: 2413 WWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFI 2472
            +W  +                  + IS ++ P GI   TL    I GLY++ VL+VGRF+
Sbjct: 2936 FWQVNAYDSTKTYNISTLESIQFYTISSKL-PNGIT-STLVSAGIIGLYVSVVLSVGRFL 2995

BLAST of Lcy05g006840 vs. ExPASy Swiss-Prot
Match: Q9H5I5 (Piezo-type mechanosensitive ion channel component 2 OS=Homo sapiens OX=9606 GN=PIEZO2 PE=1 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-50
Identity = 225/960 (23.44%), Postives = 425/960 (44.27%), Query Frame = 0

Query: 1548 SIGNQAVSNLASFLNVSQDDDMNEQTNTE--DRVYDQIESQETRYAHLERSSSLQSDKSS 1607
            S G+Q  S   + + VS DD   ++  +     +  ++ + E     +     L+  +  
Sbjct: 1841 SFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKF 1900

Query: 1608 DPASMQLGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGP 1667
                 +   +F  +++ + + +++VCY   IL  + + S++++     +F++A+     P
Sbjct: 1901 YVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRP 1960

Query: 1668 GYMFWVVMLIYTELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLF 1727
               FW++ ++YTE+ ++++Y +Q         ++ +  ++  +    I          L+
Sbjct: 1961 SRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVN--KDKPYHPPNIIGVEKKEGYVLY 2020

Query: 1728 -LVYLFTL-LQSSITAKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMF 1787
             L+ L  L    SI    G W   +   +S + R++S     L          GR+    
Sbjct: 2021 DLIQLLALFFHRSILKCHGLWD-EDDMTESGMAREESDDELSLGH--------GRRDSSD 2080

Query: 1788 VLRSLCKYWKSLTQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKE 1847
             L+S+     +L    ES    + V+     +PE      R  SG +            E
Sbjct: 2081 SLKSI-----NLAASVES----VHVT-----FPEQQTAVRRKRSGSS-----------SE 2140

Query: 1848 ENPRSCPFSSRVHVQSIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEI 1907
             + RS  FSS    +    ++ +++    VL +           +  + L  A     + 
Sbjct: 2141 PSQRS-SFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKK 2200

Query: 1908 RLAQRNGFVENTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEF 1967
             L     +V    F Y ++          D+Y  +F AD + F  +   + +  K+++  
Sbjct: 2201 TL---EIYVPIKQFFYNLIH--PEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAA 2260

Query: 1968 LDVYQL-EDQFPKEFVFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYA 2027
                 L EDQ P  F+ +++I F  +V+DR +YL     GKVIF     V+  + +  + 
Sbjct: 2261 DITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQ----VILVFGIHFWM 2320

Query: 2028 WQIEP---SNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY 2087
            + I P     + +  L  +  +  K V   L A QIR G P  + +   FLT   + +N 
Sbjct: 2321 FFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYP--TRVLGNFLTKSYNYVNL 2380

Query: 2088 LGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC-DAVLNRSQHKQ 2147
              ++ +R +PFL ELR V+DW  T T+L++  W+ +EDI A ++++KC      R    +
Sbjct: 2381 FLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPR 2440

Query: 2148 GEKQTVMTKCCNGICLFFILICVIWAPMLMYS-SGNPTNVANPIKDASCQVDIKTTSGRL 2207
            G+K+  + K   G  +  +LIC++W P+L  S   +   V N   D S  + +       
Sbjct: 2441 GQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIF 2500

Query: 2208 TL----YQTTLCEKISWDKLNANVVLDPGG--YLSPYNKNDIQLICCQADASVLWLVPGV 2267
            T+     Q  + ++ S++K       D G   +L  Y K DI +   + +++ LW +   
Sbjct: 2501 TMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPP 2560

Query: 2268 VQSRFIHS-LDRNQDISIAFTWILTRERPKG-KEVVRYDRL---VDIPDQSDVQKVLNG- 2327
             + + IH  LD N   S+ F+W + R    G K  +  D+L   +    + ++ K++ G 
Sbjct: 2561 SKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGN 2620

Query: 2328 ----SMNGFRIKNVYQRYFRVTGSGDVRPLEQ---EDSFVSADLILNRE-----NHEWWS 2387
                S     I+ +Y  Y +     + +P++Q   E++F+   +IL+R+     N EWW 
Sbjct: 2621 STESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWV 2680

Query: 2388 FHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQ 2447
             +       +        V F  ++++ P  +    L+ + I GLY + VL +G+F+R  
Sbjct: 2681 LNLTGNRIYNPNSQALELVVF--NDKVSPPSL--GFLAGYGIMGLYASVVLVIGKFVREF 2740

Query: 2448 CSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2474
             S +   I +E LP+ DR++ +C DI+  R  GEL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2741 FSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTR 2748

BLAST of Lcy05g006840 vs. ExPASy Swiss-Prot
Match: M9MSG8 (Piezo-type mechanosensitive ion channel component OS=Drosophila melanogaster OX=7227 GN=Piezo PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 7.5e-49
Identity = 161/613 (26.26%), Postives = 288/613 (46.98%), Query Frame = 0

Query: 1923 SVIGGGKREIDLYAYVFGADLIVFFFVAIF----YQSVIKNNSEFLDVYQLEDQFPKEFV 1982
            S++   +   D+YA +F  D  V FFV +F    + +    + E +  Y  E++ P  F+
Sbjct: 1924 SLVHKSRLATDVYALMFLCD-FVNFFVLLFGFTAFGTQQTESDEGVQTYLAENKVPIPFL 1983

Query: 1983 FILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEP--SNQHAGELA 2042
             +L++ F LIV+DR +YL      K+IF+ F+++     +  + + + P  + +    LA
Sbjct: 1984 IMLLVQFLLIVIDRALYLRKALVNKIIFHFFSVI----GIHIWMFFVVPAVTERTFNSLA 2043

Query: 2043 LRAIF-LAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELR 2102
               IF + K   + L + QI+ G P +  +   F T   S +N + +++Y  +PFLYELR
Sbjct: 2044 PPIIFYVIKCFYMLLSSYQIKSGYPKR--ILGNFFTKGFSMVNMIAFKVYMQIPFLYELR 2103

Query: 2103 CVLDWSCTTTSLTMYDWLKLEDINASLYLVKC------DAVLNRSQHKQGEKQTVMTKCC 2162
             +LDW C  +++T++DWLK+EDI +++YL++C      D    R+Q K    + +M    
Sbjct: 2104 TILDWVCIDSTMTIFDWLKMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIM---- 2163

Query: 2163 NGICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQV------DIKTTSGRLTLYQTT 2222
             G  +  I+IC IW P+ +++ GN    +N     S  +       I TT+   ++++  
Sbjct: 2164 GGTIVLLIVIC-IWGPLCLFALGNAVGTSNVPFHVSLSIRIGPYDPIYTTNNYDSIFE-- 2223

Query: 2223 LCEKISWDKLNANVVLDPG-GYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDR 2282
            +  ++     NA +       +++ Y+  D+  +    ++  LW +    + R ++ L  
Sbjct: 2224 INPEMYSQMTNAYIKEKQALTFIAGYDATDVAAVRLAGNSPSLWNIAPPDRQRLLNDLRN 2283

Query: 2283 NQDISIAFTWILTRERP-KG-KEVVRYDRLVDIPDQ--------------SDVQKVL-NG 2342
            N  +   F++ LTR+ P KG KE V  +  + + +                DV+ +  NG
Sbjct: 2284 NHTLKARFSYSLTRKAPAKGLKENVGDEHAISLDESFEGRAALIHMLSETHDVEPIYSNG 2343

Query: 2343 SMNGFR--------IKNVYQRYFRVTGSGD--VRPLEQEDSFVSADLIL-----NRENHE 2402
            + NG          I  +  ++ +V  SGD  V  +     +    L++     N  N  
Sbjct: 2344 TTNGTTPEVEEVVVIPGMIPKFIKVLNSGDAAVVSVLSPKHYDYRPLVIKMHRDNETNGL 2403

Query: 2403 WWSFHD----------IQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIWGLYI 2462
            WW   D          +     S C   +G V +  +++  P       L+   I GLY 
Sbjct: 2404 WWEIRDYCNDTFYNETLSKFAYSNC--TSGIVMYTFNDKKFPSTF--SFLTAGGIIGLYT 2463

Query: 2463 TFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYWTLVK 2474
            TFVL   RF++        +I +E+LP  DR++ +C DIY  R   E  +EE L+  L+ 
Sbjct: 2464 TFVLLASRFMKSFIGGQNRKIMFEDLPYVDRVLQLCLDIYLVREALEFALEEDLFAKLLF 2518

BLAST of Lcy05g006840 vs. ExPASy Swiss-Prot
Match: Q0KL00 (Piezo-type mechanosensitive ion channel component 1 OS=Rattus norvegicus OX=10116 GN=Piezo1 PE=1 SV=3)

HSP 1 Score: 195.7 bits (496), Expect = 6.3e-48
Identity = 156/585 (26.67%), Postives = 272/585 (46.50%), Query Frame = 0

Query: 1933 DLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQ--LEDQFPKEFVFILMIIFFLIVL 1992
            D+YA +F AD IV   V IF       +S   D+     +DQ P+ F+F+L++ F  +V+
Sbjct: 1963 DVYALMFLAD-IVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVI 2022

Query: 1993 DRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPS---NQHAGELALRAIFLAKAVS 2052
            DR +YL     G + F     V+   A+  + + I P+            +  +  K + 
Sbjct: 2023 DRALYLRKTVLGNLAFQ----VVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVKCIY 2082

Query: 2053 LALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSL 2112
             AL A QIR G P  + +   FLT + + +N   ++ +R +PFL ELR V+DW  T T+L
Sbjct: 2083 FALSAYQIRCGYP--TRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTL 2142

Query: 2113 TMYDWLKLEDINASLYLVKCDAVLNRS-QHKQGEKQTVMTKCCNGICLFFILICVIWAPM 2172
            ++ +W+ +EDI A+++++KC     +     +G+K+  + K   G  +   LI +IW P+
Sbjct: 2143 SLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPL 2202

Query: 2173 LMYS-SGNPTNVANPIKDASCQVDIKTTSGRLTL--YQTTLCEKISWDKLNANVVLDP-- 2232
            L  S   +   V N   D +  + +       T+   Q ++      D    +   DP  
Sbjct: 2203 LFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQFDPYP 2262

Query: 2233 --GGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSL-DRNQDISIAFTWILTRER 2292
                ++S Y+  DI     +  +  LW +    +++  H L +   DI++ FTW   R+ 
Sbjct: 2263 LAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNFQRDL 2322

Query: 2293 PKG--KEVVRYDRLVDIPDQSDVQKVLNGSMNG-----FRIKNVYQRYFRVTGSGDVRPL 2352
             KG   E       +++   S  ++ L   + G       I +++ +Y R     +  P+
Sbjct: 2323 AKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPV 2382

Query: 2353 EQ-----EDSFVSADLILNREN-----------------HEWWSFHDIQPINVSKCGHFT 2412
            +Q     E+ ++   + L RE                   EWW       I +  C    
Sbjct: 2383 KQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWW------VIELQDCQAEC 2442

Query: 2413 GPVAFVI-SEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPS 2472
              +  VI S+++ P  +    L+ + I GLY++ VL VG+F+R   SD+   I +E LP 
Sbjct: 2443 NLLPMVIFSDKVSPPSL--GFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMFEELPC 2502

Query: 2473 CDRLIAICEDIYAARAEGELGVEEVLYWTLVKIYRSPHMLLEYTK 2474
             DR++ +C+DI+  R   EL +EE LY  L+ +YRSP  ++++T+
Sbjct: 2503 VDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTR 2532

BLAST of Lcy05g006840 vs. ExPASy TrEMBL
Match: A0A1S3BRG7 (LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel homolog OS=Cucumis melo OX=3656 GN=LOC103492709 PE=3 SV=1)

HSP 1 Score: 4575.0 bits (11865), Expect = 0.0e+00
Identity = 2317/2479 (93.47%), Postives = 2398/2479 (96.73%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSLVSLTDLLVFLFI FN HKIGFIFGRRFLVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFL+IWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILAQVTFLLIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            TADI+ YRIGLVPRGDSCW+HF SF++ LGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFWYRIGLVPRGDSCWIHFFSFVNHLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKISS SEWP+ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSNSEWPEICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLCLSGEDE SSNDNSS  EAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSSNDNSSPGEAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDV+RKMRQSLILLNVVHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVTRKMRQSLILLNVVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI L+S+VLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPPVGFSILRAG NKSVLLSVYTATTNNY HD+PSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VEHTPSPPVGFSILRAGLNKSVLLSVYTATTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNY+SFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYLSFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            YSSSDEPELLE+ RLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP
Sbjct: 781  YSSSDEPELLEFGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS LSYFLG R F+PG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSYFLGLREFQPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGSVLNKGKWDDPCPLFV+EEDD YDIS ++EKNK
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVTEEDD-YDISISNEKNK 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
              LDSGRLF   EG DHI R SF   Q+ V +SASSKR+NSECSS++K SFGF+WGSTKE
Sbjct: 961  SPLDSGRLFEQQEGLDHIRRPSFVSAQNQVPHSASSKRDNSECSSNRKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SHKWDK+RIISLRKERFE+QKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS
Sbjct: 1021 SHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWHMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTY KMM
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYHKMM 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            SQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVS+QSYMFSSQEF+YVCRYLEAEQIGAIVREQEKK 
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSVQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKX 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNN SPSPGNES R
Sbjct: 1381 AWKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNVSPSPGNESFR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMELP 1500
             R TSRIHDDAMT DIEGTLGKVEQI+REDS PHPELQESLASLR  +TTESRMHSME P
Sbjct: 1441 MRSTSRIHDDAMTTDIEGTLGKVEQIIREDSSPHPELQESLASLRAGLTTESRMHSMESP 1500

Query: 1501 VIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLAS 1560
            V EICEVDSQISD+SLDLDRKRKHKGSAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLAS
Sbjct: 1501 VAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLAS 1560

Query: 1561 FLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620
            FLNV+QDDD+NEQ+ TED VYDQIE QETRYAHLERSSSLQSD+SSDPASMQLGRIFRHI
Sbjct: 1561 FLNVTQDDDINEQSKTEDGVYDQIEIQETRYAHLERSSSLQSDRSSDPASMQLGRIFRHI 1620

Query: 1621 WSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680
            W+QMR+NNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL
Sbjct: 1621 WAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680

Query: 1681 YVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740
            Y+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA
Sbjct: 1681 YILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740

Query: 1741 KDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800
            KDGEW YS+AFNK+ALPRKQS GHYGLT+RAYELLY+GRKMMMFVLRSLCKYWKSLTQGA
Sbjct: 1741 KDGEWAYSSAFNKNALPRKQSFGHYGLTDRAYELLYIGRKMMMFVLRSLCKYWKSLTQGA 1800

Query: 1801 ESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQS 1860
            ESPPYFIQVS+DVQIWPEDGIQPERIESGIN +LQIIHVERCKE+NPR CPFSSRVHVQS
Sbjct: 1801 ESPPYFIQVSLDVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCPFSSRVHVQS 1860

Query: 1861 IVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFPY 1920
            I RSKENTKVALVVLEVVYSSPS +TCAEWCDSLTPAADVANEIRLAQRNGFVE+TGFPY
Sbjct: 1861 IERSKENTKVALVVLEVVYSSPSTNTCAEWCDSLTPAADVANEIRLAQRNGFVESTGFPY 1920

Query: 1921 QILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980
            QILSVIGGGKREIDLYAYVFGAD+IVFF VAIFYQS+IKNNSEFLDVYQLEDQFPKEFVF
Sbjct: 1921 QILSVIGGGKREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVF 1980

Query: 1981 ILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALRA 2040
            +LMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGELALRA
Sbjct: 1981 VLMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRA 2040

Query: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100
            IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLD
Sbjct: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLD 2100

Query: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFILI 2160
            WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQT+MTKCCNGICLFFILI
Sbjct: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTIMTKCCNGICLFFILI 2160

Query: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVVL 2220
            CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLN N+VL
Sbjct: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVL 2220

Query: 2221 DPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRERP 2280
            DPGGYLSPYN++DIQLICCQADASVLWLVP VVQS FIHSLDR QDI I+FTWILTR+RP
Sbjct: 2221 DPGGYLSPYNQDDIQLICCQADASVLWLVPDVVQSGFIHSLDRKQDIIISFTWILTRDRP 2280

Query: 2281 KGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQEDS 2340
            KGKEVV+YDR++   D+P+QSDVQKVLNGSMNGFRIKNVYQRYFRVTGSG+VRPLEQE+S
Sbjct: 2281 KGKEVVKYDRVIEPRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEES 2340

Query: 2341 FVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400
            FVSADLILNR N+EWWSFHDIQPINVS+CG FTGPVAFVISEEIPPQGILGDTLSKFSIW
Sbjct: 2341 FVSADLILNRNNYEWWSFHDIQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400

Query: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460
            GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW
Sbjct: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460

Query: 2461 TLVKIYRSPHMLLEYTKID 2476
            TLVKIYRSPHMLLEYTKID
Sbjct: 2461 TLVKIYRSPHMLLEYTKID 2478

BLAST of Lcy05g006840 vs. ExPASy TrEMBL
Match: A0A6J1JXP1 (piezo-type mechanosensitive ion channel homolog OS=Cucurbita maxima OX=3661 GN=LOC111488810 PE=3 SV=1)

HSP 1 Score: 4482.9 bits (11626), Expect = 0.0e+00
Identity = 2277/2480 (91.81%), Postives = 2366/2480 (95.40%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSL+SLTDLL+FLFI FN  KIGFIFGRRFLV WPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVFWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLL+I VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADI+ YRIG V RGDSCWVHF SF+D LGSHLRVASCLLLPAIQIIVGIS+PSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRGDSCWVHFFSFVDHLGSHLRVASCLLLPAIQIIVGISNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+SSTSEWP ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTSEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLN+KIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNQKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC+S EDERSSNDN SAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+VSRKMRQSLILLN +HFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI+L+S+VLDREGSLCREILLQLGLLGRDSMWEFLEI +LA FCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEITMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAG NKSVLLSVYT TTNNY HD+PSHER+IASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERQIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF SFRIWLSRSIDL F+LGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            Y+SSDEPE LEY RLGF+KRFL WHSDKILFA+LFYASISPISAFGLLYLLGL +C+TLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS+LSYFLGFRVFEPG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGS LNKGKWDDPCPLFVS+EDD YDIS +HEKN 
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSALNKGKWDDPCPLFVSDEDD-YDISISHEKNN 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
            PSLD+GRLFV  EGSDHI R SF   QS V +SASSKR+NS CSS+ K SFGF+WGSTKE
Sbjct: 961  PSLDAGRLFVQQEGSDHILRPSFVSNQSQVPHSASSKRDNSRCSSTSKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
             HKWDKMRII  RKERFEMQKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA+LNSVS
Sbjct: 1021 CHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTYRKM+
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMV 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            S RKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEF+YVCRYLEAEQIGAIV EQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KR+RNLQVEKMKSEMLN QIQLHNMNSS DGNNASP PGNE LR
Sbjct: 1381 AWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASPIPGNEFLR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGT-LGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMEL 1500
            K+ TSRI DDAMT DIEGT LGKVEQ++R DS P PELQESLA+LR    TESRMHSME 
Sbjct: 1441 KQSTSRIDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHSMEF 1500

Query: 1501 PVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLA 1560
             V EICE+DSQISD+SLDLDRKRKHK SAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLA
Sbjct: 1501 SVPEICEIDSQISDISLDLDRKRKHKSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560

Query: 1561 SFLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRH 1620
            SFLNV+QDDDM EQ NTEDRVYDQIESQETRY H ERSSSL SDKSSDPA+MQLGRIFRH
Sbjct: 1561 SFLNVTQDDDMIEQRNTEDRVYDQIESQETRYTHFERSSSLHSDKSSDPATMQLGRIFRH 1620

Query: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTE 1680
            IWSQMRSNNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWV+MLIYTE
Sbjct: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTE 1680

Query: 1681 LYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSIT 1740
            LY+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFV+SSLPLFLVYLFTLLQSSIT
Sbjct: 1681 LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSIT 1740

Query: 1741 AKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQG 1800
            AKDGEWTYSNAFNKS LPRK SLGHYGLTE+AYELL+LG KMMMFVLRSLCKYWKSLTQG
Sbjct: 1741 AKDGEWTYSNAFNKSDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTQG 1800

Query: 1801 AESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQ 1860
            AESPPYFIQVSMDVQ+WPE GIQPERIESGINQ+LQIIH  RCKE+NPR CPFSSRVHVQ
Sbjct: 1801 AESPPYFIQVSMDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQ 1860

Query: 1861 SIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFP 1920
            SI RSKEN K+ALVVLEVVYSSPS DTCAEW DSLTPAADV NEIRLAQ  G VENTGFP
Sbjct: 1861 SIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFP 1920

Query: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFV 1980
            YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNN+EFLDVYQLEDQFPKEFV
Sbjct: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFV 1980

Query: 1981 FILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALR 2040
            FILMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTY VT+YAWQ+EPSNQHAGELALR
Sbjct: 1981 FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYVVTEYAWQMEPSNQHAGELALR 2040

Query: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100
            AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL
Sbjct: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100

Query: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFIL 2160
            DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQTVMTKCCNGICLFFIL
Sbjct: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFIL 2160

Query: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVV 2220
            ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKT+SGRLTLYQTTLCEKI W+KLNAN+V
Sbjct: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMV 2220

Query: 2221 LDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRER 2280
            LDPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLD NQDISIAFTW+LTR+R
Sbjct: 2221 LDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDR 2280

Query: 2281 PKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQED 2340
            PKGKEVVRYDRLV   D+P+QSD+QKVLNGSMN F+IKNVY+RYFRVTGSGDVRPLEQED
Sbjct: 2281 PKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQED 2340

Query: 2341 SFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSI 2400
            +FVSADLILNREN++WWSFHDIQPINVSKCGHFTGPVA VISEEIPPQGILGDTLSKFSI
Sbjct: 2341 NFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSI 2400

Query: 2401 WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY 2460
            WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY
Sbjct: 2401 WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY 2460

Query: 2461 WTLVKIYRSPHMLLEYTKID 2476
            WTLVKIYRSPHMLLEYTKID
Sbjct: 2461 WTLVKIYRSPHMLLEYTKID 2475

BLAST of Lcy05g006840 vs. ExPASy TrEMBL
Match: A0A6J1GNI2 (piezo-type mechanosensitive ion channel homolog OS=Cucurbita moschata OX=3662 GN=LOC111456038 PE=3 SV=1)

HSP 1 Score: 4476.4 bits (11609), Expect = 0.0e+00
Identity = 2276/2481 (91.74%), Postives = 2366/2481 (95.36%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSL+SLTDLL+FLFI FN  KIGFIFGRRFLVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLL+I VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADI+ YRIG V R DSCWVH  SF+D LGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+SST EWP ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC+S EDERSSNDN SAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+VSRKMRQSLILLN +HFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI+L+S+VLDREGSLCREILLQLGLLGRDSMWEFLEIA+LA FCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAG NKSVLLSVYT TTNNY HD+PSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVER KRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF SFRIWLSRSIDL F+LGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            Y+SSDEPE LEY RLGF+KRFL WHSDKILFA+LFYASISPISAFGLLYLLGL +C+TLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS+LSYFLGFRVFEPG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGSVLNKGKWDDPCPLFVS+EDD +DIS +HEKN 
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDD-FDISISHEKNN 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
            PSLD+GRLFV  EGSDHI R SF   QS V +SASSKR+NSECSS+ K SFGF WGSTKE
Sbjct: 961  PSLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKRDNSECSSTNKYSFGFTWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
             HKWDKMRII  RKERFEMQKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA+LNSVS
Sbjct: 1021 CHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTYRKM+
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMV 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            S RKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEF+YVCRYLEAEQIGAIV EQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNA-SPSPGNESL 1440
            AWKTEQLQHIRDSEE KR+RNLQVEKMKSEMLN QIQLHNMNSS DGNNA SP PGNE L
Sbjct: 1381 AWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFL 1440

Query: 1441 RKRGTSRIHDDAMTIDIEGT-LGKVEQILREDSFPHPELQESLASLRTEITTESRMHSME 1500
            RK+ TSR+ DDAMT DIEGT LGKVEQ++R DS P PELQESLA+LR    TESRMHSME
Sbjct: 1441 RKQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHSME 1500

Query: 1501 LPVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNL 1560
              V EICE+DSQISD+SLDLDRKRKH+ SAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNL
Sbjct: 1501 FSVPEICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNL 1560

Query: 1561 ASFLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFR 1620
            ASFLNV+QDDDM EQ NTEDRVYDQIESQETRY H ERSSSLQSDKSSDPA+MQLGRIFR
Sbjct: 1561 ASFLNVTQDDDMIEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFR 1620

Query: 1621 HIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYT 1680
            H+WSQMRSNNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWV+MLIYT
Sbjct: 1621 HVWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYT 1680

Query: 1681 ELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSI 1740
            ELY+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFV+SSLPLFLVYLFTLLQSSI
Sbjct: 1681 ELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSI 1740

Query: 1741 TAKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQ 1800
            TAKDGEWTYSNAFNK+ALPRK SLGHYGLTE+AYELL+LG KMMMFVLRSLCKYWKSLT+
Sbjct: 1741 TAKDGEWTYSNAFNKNALPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTK 1800

Query: 1801 GAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHV 1860
            GAESPPYFIQVS+DVQ+WPE GIQPERIESGINQ+LQIIH  RCKE+NPR CPFSSRVHV
Sbjct: 1801 GAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHV 1860

Query: 1861 QSIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGF 1920
            QSI RSKEN K+ALVVLEVVYSSPS DTCAEW DSLTPAADV NEIRLAQ  G VENTGF
Sbjct: 1861 QSIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGF 1920

Query: 1921 PYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEF 1980
            PYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNN+EFLDVYQLEDQFPKEF
Sbjct: 1921 PYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEF 1980

Query: 1981 VFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELAL 2040
            VFILMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVTKYAWQ+EPSNQHAGELAL
Sbjct: 1981 VFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELAL 2040

Query: 2041 RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV 2100
            RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV
Sbjct: 2041 RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV 2100

Query: 2101 LDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFI 2160
            LDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLFFI
Sbjct: 2101 LDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFI 2160

Query: 2161 LICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANV 2220
            LICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKT+SGRLTLYQTTLCEKI W+KLNAN+
Sbjct: 2161 LICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANM 2220

Query: 2221 VLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRE 2280
            VLDPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLD NQDISIAFTW+LTR+
Sbjct: 2221 VLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRD 2280

Query: 2281 RPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQE 2340
            RPKGKEVVRYDRLV   D+P+QSD+QKVLNGSMN F+IKNVY+RYFRVTGSGDVRPLEQE
Sbjct: 2281 RPKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQE 2340

Query: 2341 DSFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFS 2400
            D+FVSADLILNREN++WWSFHDIQPINVSKCGHFTGPVA VISEEIPPQGILGDTLSKFS
Sbjct: 2341 DNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFS 2400

Query: 2401 IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL 2460
            IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL
Sbjct: 2401 IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL 2460

Query: 2461 YWTLVKIYRSPHMLLEYTKID 2476
            YWTLVKIYRSPHMLLEYTKID
Sbjct: 2461 YWTLVKIYRSPHMLLEYTKID 2476

BLAST of Lcy05g006840 vs. ExPASy TrEMBL
Match: A0A6J1KQD8 (piezo-type mechanosensitive ion channel homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497741 PE=3 SV=1)

HSP 1 Score: 4471.8 bits (11597), Expect = 0.0e+00
Identity = 2281/2481 (91.94%), Postives = 2371/2481 (95.57%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSLV+L DLLVFLFI FN HKIGFIFGRRFLVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVALADLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI+SQVTFLVIWSLEGY WS+ANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILSQVTFLVIWSLEGYNWSLANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADIY YRIGLVPRGDSCWV F SF+DRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIYWYRIGLVPRGDSCWVQFSSFVDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVY YQLPV YPSMLKWVA+FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYAYQLPVGYPSMLKWVAKFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
             KISSTSEWP+ICSYVSLILFYIMLSCVKCDLEEMDFIMS RESNLVEQLLPSKHSFFIR
Sbjct: 241  SKISSTSEWPEICSYVSLILFYIMLSCVKCDLEEMDFIMSTRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGV+HTNVLLRR+VFRTFTINFFTYGFPV+LVALSFWSFHFASLCAFGLLAYVGYI+
Sbjct: 301  ELRSGVRHTNVLLRRDVFRTFTINFFTYGFPVALVALSFWSFHFASLCAFGLLAYVGYIV 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            Y FPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDM VWEMVGLWHYPIPG
Sbjct: 361  YVFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMHVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLC S EDERSSN+NSS REAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCSSDEDERSSNENSSVREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAI+LTLIFLVAMKPG IHAVYVVFFLLYLLSHDV RKMRQ++ILLN VHFALLYLL
Sbjct: 481  KSSRAILLTLIFLVAMKPGLIHAVYVVFFLLYLLSHDVGRKMRQAIILLNEVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLG-RDSMWEFLEIALLACFCTIHNHGFEMLFSFSA 600
            QI+LVSDVL REGSLCREILLQLGLLG RDS WEFLEIALLACFC+IHNHGFEMLFSFSA
Sbjct: 541  QINLVSDVLGREGSLCREILLQLGLLGRRDSTWEFLEIALLACFCSIHNHGFEMLFSFSA 600

Query: 601  IVQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSM 660
            +VQHTPSPPVGFSILRAG NKSVLLSVYTAT+NNY HD+PSHERKIASFLSSIGEKFLSM
Sbjct: 601  VVQHTPSPPVGFSILRAGLNKSVLLSVYTATSNNYCHDNPSHERKIASFLSSIGEKFLSM 660

Query: 661  YRSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANI 720
            YRSCGTYIAFLTILLTV FVKPNYISFG+LFLLLVWIIGRQLVERTKRRLWFPLKAYA I
Sbjct: 661  YRSCGTYIAFLTILLTVCFVKPNYISFGYLFLLLVWIIGRQLVERTKRRLWFPLKAYAII 720

Query: 721  VFVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQS 780
            VF+FIYC+SSFSSFRIWLSRSIDL+FYLGY+SEASSLQNCWQSLAV+IVMQLYSYERRQS
Sbjct: 721  VFIFIYCMSSFSSFRIWLSRSIDLNFYLGYDSEASSLQNCWQSLAVMIVMQLYSYERRQS 780

Query: 781  RYSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATL 840
            RY SSDEP LLEY   GFIKRFLIWH DKILFAALFYASISPISAFGLLYLLGLV+CATL
Sbjct: 781  RYLSSDEPGLLEYG--GFIKRFLIWHCDKILFAALFYASISPISAFGLLYLLGLVVCATL 840

Query: 841  PKTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLE 900
            PK SHIPSKLFLAYTGVIMTAEYLFQMWG+QAEMFPGQKH+ LSYFLGFRVF PG WGLE
Sbjct: 841  PKASHIPSKLFLAYTGVIMTAEYLFQMWGEQAEMFPGQKHAHLSYFLGFRVFRPGFWGLE 900

Query: 901  LGLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYY-DISFTHEK 960
            LGLR KVL+IAA TLQYNVFRWLE++PGSVLNKGKWDDPCPLFVSEEDDYY DIS ++EK
Sbjct: 901  LGLRGKVLVIAASTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVSEEDDYYDDISSSNEK 960

Query: 961  NKPSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGST 1020
            NKPS DSGR FV  E SDHIS SSFA G++ + + ASSKRENSECSSSKKS FGF+WGS 
Sbjct: 961  NKPSFDSGRSFVPQEVSDHISSSSFASGRAQMPHDASSKRENSECSSSKKSPFGFIWGSI 1020

Query: 1021 KESHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNS 1080
            KESHKWDK RI SLRKERFEMQKIIF+IYMKFWMENLF LFGLEITMISLLL SFALLNS
Sbjct: 1021 KESHKWDKTRITSLRKERFEMQKIIFEIYMKFWMENLFYLFGLEITMISLLLTSFALLNS 1080

Query: 1081 VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWQ-PSKAGVH 1140
            VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNM NSNW  PSKA VH
Sbjct: 1081 VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMMNSNWLIPSKADVH 1140

Query: 1141 CHDCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRK 1200
            CHDCWRI+NLY++FCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRAD LSGF LSSTY K
Sbjct: 1141 CHDCWRISNLYYKFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADRLSGFSLSSTYHK 1200

Query: 1201 MMSQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL 1260
            MMSQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL
Sbjct: 1201 MMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL 1260

Query: 1261 AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM 1320
            AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM
Sbjct: 1261 AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM 1320

Query: 1321 IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEK 1380
            IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEF+YVCRYLEAEQIGAIVREQEK
Sbjct: 1321 IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVREQEK 1380

Query: 1381 KAAWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNES 1440
            KAAWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNSSV+GNNASPSPG+ES
Sbjct: 1381 KAAWKTEQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSSVNGNNASPSPGDES 1440

Query: 1441 LRKRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSME 1500
            LRKR TSRIHDDAMT DIEGT GKVEQI+R+DS    ELQESLA+LRTEITTESR  SME
Sbjct: 1441 LRKRSTSRIHDDAMTTDIEGTHGKVEQIIRDDSSSQLELQESLANLRTEITTESRTQSME 1500

Query: 1501 LPVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNL 1560
            LPV +ICE++SQISD+SLD DRKRKHKGS KGNPL+SAVQFIGDGVSQVQSIGNQAVSNL
Sbjct: 1501 LPVADICEINSQISDVSLDSDRKRKHKGSTKGNPLMSAVQFIGDGVSQVQSIGNQAVSNL 1560

Query: 1561 ASFLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFR 1620
            A+FLNV+QDDDMNEQTNTEDRVYDQIESQ+ RYA LERSSSLQSDKSSDPASMQLGRIF 
Sbjct: 1561 ATFLNVTQDDDMNEQTNTEDRVYDQIESQQPRYADLERSSSLQSDKSSDPASMQLGRIFH 1620

Query: 1621 HIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYT 1680
            +IWSQMRSNNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVN GPGYMFWVVMLIYT
Sbjct: 1621 YIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNAGPGYMFWVVMLIYT 1680

Query: 1681 ELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSI 1740
            ELY+LLQYLYQIIIQHCGLAI+SDLL+ELGFPT+RITSSFVVSSLPLFLVYLFTLLQ SI
Sbjct: 1681 ELYILLQYLYQIIIQHCGLAINSDLLQELGFPTNRITSSFVVSSLPLFLVYLFTLLQRSI 1740

Query: 1741 TAKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQ 1800
            TAKDGEWTY NAFNKSALPRK SLG YGLTERAYE LY+GRKM+M +LRS  KYWKSLTQ
Sbjct: 1741 TAKDGEWTYLNAFNKSALPRKHSLGRYGLTERAYEFLYVGRKMVMVMLRSSWKYWKSLTQ 1800

Query: 1801 GAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHV 1860
            GAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLL+IIH ERCK++N R CPF SRVHV
Sbjct: 1801 GAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLRIIHEERCKKQNARLCPFYSRVHV 1860

Query: 1861 QSIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGF 1920
            QSI RSKENT+VALVVLEV YSSPS+DTC+EWCDSLTPAADVANEIRLAQRNGFVENTGF
Sbjct: 1861 QSIERSKENTEVALVVLEVEYSSPSSDTCSEWCDSLTPAADVANEIRLAQRNGFVENTGF 1920

Query: 1921 PYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEF 1980
            PY+ILSVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEF
Sbjct: 1921 PYRILSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEF 1980

Query: 1981 VFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELAL 2040
            VF+LMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGELAL
Sbjct: 1981 VFVLMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELAL 2040

Query: 2041 RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV 2100
            RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV
Sbjct: 2041 RAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCV 2100

Query: 2101 LDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFI 2160
            LDWSCT+TSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFI
Sbjct: 2101 LDWSCTSTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFI 2160

Query: 2161 LICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANV 2220
            L+ VIWAPMLMYSSGNPTNV NPIKDASCQVDIKTTSGRLTLYQTTLCEKISW+ LNAN+
Sbjct: 2161 LLFVIWAPMLMYSSGNPTNVENPIKDASCQVDIKTTSGRLTLYQTTLCEKISWNNLNANL 2220

Query: 2221 VLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRE 2280
            VLDPGGYL PYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQ+IS++FTWILTR+
Sbjct: 2221 VLDPGGYLGPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQNISVSFTWILTRD 2280

Query: 2281 RPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQE 2340
            RPKGKEVV+YDR+V   D+P+ SDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQE
Sbjct: 2281 RPKGKEVVQYDRVVDPRDLPNPSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQE 2340

Query: 2341 DSFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFS 2400
            DSFV+ADLILNREN+EWWSFHDIQPINVS+CGHFTGPVAFVISEEIPPQGILGDTLSKFS
Sbjct: 2341 DSFVNADLILNRENYEWWSFHDIQPINVSECGHFTGPVAFVISEEIPPQGILGDTLSKFS 2400

Query: 2401 IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL 2460
            IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL
Sbjct: 2401 IWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVL 2460

Query: 2461 YWTLVKIYRSPHMLLEYTKID 2476
            YWTLVKIYRSPHMLLEYTKID
Sbjct: 2461 YWTLVKIYRSPHMLLEYTKID 2479

BLAST of Lcy05g006840 vs. ExPASy TrEMBL
Match: A0A6J1D7Y8 (piezo-type mechanosensitive ion channel homolog OS=Momordica charantia OX=3673 GN=LOC111018458 PE=3 SV=1)

HSP 1 Score: 4455.6 bits (11555), Expect = 0.0e+00
Identity = 2279/2485 (91.71%), Postives = 2365/2485 (95.17%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALIN SLVSL DLLVFLFI FN HKIGFIFGRR LVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINCSLVSLIDLLVFLFIQFNAHKIGFIFGRRNLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI+SQ  FL++WSLEGY  S+ANAWW K+IGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILSQGIFLLVWSLEGYNISLANAWWAKVIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            TADI+R RIGLVPRGDSCWVH LSF D+LGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFRDRIGLVPRGDSCWVHLLSFFDQLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPV+YPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVQYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKI STSEWP+ICSYVSL LFYIMLSCVKCDLEEMDFI+SMRE+NLVEQLLPSKHSFFIR
Sbjct: 241  FKILSTSEWPEICSYVSLTLFYIMLSCVKCDLEEMDFILSMRENNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF LHRLNGLLLVFILFWAISTYIFNVAFTFLNRK+GKDMDVWEM+GLWHYPIPG
Sbjct: 361  YAFPSLFHLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKLGKDMDVWEMMGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSN-----DNSSAREAGETKVLIVATI 480
            FFLLAQF LGILVALVNLVNNSVFLCLS EDE SSN     DNSSAREAGETKVLIVATI
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCLSNEDECSSNDNSNADNSSAREAGETKVLIVATI 480

Query: 481  AWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFA 540
            AWGLRKSSR IVLTLIFLVAMKPGFIHAVYVVFFL+YLLSHDVSRKMRQSLILLN VHF+
Sbjct: 481  AWGLRKSSRPIVLTLIFLVAMKPGFIHAVYVVFFLVYLLSHDVSRKMRQSLILLNEVHFS 540

Query: 541  LLYLLQIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLF 600
            LLYLLQI+L+SDVLDREGS CREILLQLGLLGRDSMW+FLEIALLACFC+IHNHGFEMLF
Sbjct: 541  LLYLLQINLISDVLDREGSFCREILLQLGLLGRDSMWDFLEIALLACFCSIHNHGFEMLF 600

Query: 601  SFSAIVQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEK 660
            SFSAIVQHTP PPVGFSILRAG NKSVLLSVYT+T N+Y HD+ SHERKIASFLSSIGEK
Sbjct: 601  SFSAIVQHTPCPPVGFSILRAGLNKSVLLSVYTSTKNHYGHDNSSHERKIASFLSSIGEK 660

Query: 661  FLSMYRSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKA 720
            FLSMYRSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVW+IGRQLV RTKR+LWFPLKA
Sbjct: 661  FLSMYRSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWMIGRQLVGRTKRQLWFPLKA 720

Query: 721  YANIVFVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYE 780
            YA  VFVFIYCLSSF SFRIWLSRSIDL FYLGYNSEASSL NCWQSLAVLIVMQLYSYE
Sbjct: 721  YAITVFVFIYCLSSFPSFRIWLSRSIDLYFYLGYNSEASSLHNCWQSLAVLIVMQLYSYE 780

Query: 781  RRQSRYSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGL-V 840
            RRQSRYSSSDEPELLEY R GFIKRFLIWH DKILFAALFYASISPISAFGLLYLLGL V
Sbjct: 781  RRQSRYSSSDEPELLEYGRFGFIKRFLIWHCDKILFAALFYASISPISAFGLLYLLGLVV 840

Query: 841  ICATLPKTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPG 900
            +CATLPK SHIPSKLFLAYTGV++TAEYLFQMWGKQA MFPGQKH  LS FLG RVFEPG
Sbjct: 841  VCATLPKMSHIPSKLFLAYTGVVLTAEYLFQMWGKQAGMFPGQKHYHLSRFLGLRVFEPG 900

Query: 901  VWGLELGLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISF 960
             WGLELGLR KVLIIAACTLQYNVFRWLE++P SVLNKGKWDDPCPLFVSEE+DYYDIS 
Sbjct: 901  FWGLELGLRGKVLIIAACTLQYNVFRWLERMPSSVLNKGKWDDPCPLFVSEENDYYDISI 960

Query: 961  THEKNKPSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFM 1020
            ++E+ +PSL+SGRLF+H EGSDHISRSSFA G+S V +SASSKR+NSECSSSKK SFGF+
Sbjct: 961  SNER-RPSLNSGRLFLHPEGSDHISRSSFASGRSQVPHSASSKRDNSECSSSKKYSFGFI 1020

Query: 1021 WGSTKESHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFA 1080
            WGSTKESHKWDKMRI+SLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFA
Sbjct: 1021 WGSTKESHKWDKMRIMSLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFA 1080

Query: 1081 LLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWQP-SK 1140
            LLNSVS+FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW   SK
Sbjct: 1081 LLNSVSIFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWHMLSK 1140

Query: 1141 AGVHCHDCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSS 1200
            AGVHCHDCWR +NLYF+FCLNCWLGLTVDDSRMLFSYF+VFMLSSLKLRADHLSGF LSS
Sbjct: 1141 AGVHCHDCWRFSNLYFRFCLNCWLGLTVDDSRMLFSYFMVFMLSSLKLRADHLSGFSLSS 1200

Query: 1201 TYRKMMSQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLH 1260
            TYRKMMSQRKNT VWRDLSFETKSMWTI+DYLRLYCYCHLLDLVLALILITGTLEYDVLH
Sbjct: 1201 TYRKMMSQRKNTFVWRDLSFETKSMWTIIDYLRLYCYCHLLDLVLALILITGTLEYDVLH 1260

Query: 1261 LGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY 1320
            LGYLAFALVFFRLRLEILKKKNK+FKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY
Sbjct: 1261 LGYLAFALVFFRLRLEILKKKNKLFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY 1320

Query: 1321 IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVR 1380
            IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEF+YVCRYLEAEQIGAIV 
Sbjct: 1321 IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVH 1380

Query: 1381 EQEKKAAWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSP 1440
            EQEKKAAWKT QLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNS VD NNA  SP
Sbjct: 1381 EQEKKAAWKTSQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSFVDANNA--SP 1440

Query: 1441 GNESLRKRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRM 1500
            G+ES RKR TSR HDD MT DIEGTLGKVEQI+R+DS PHPELQES A+LRTEITTESR 
Sbjct: 1441 GDESRRKR-TSRTHDDTMTTDIEGTLGKVEQIVRQDSSPHPELQESFANLRTEITTESRT 1500

Query: 1501 HSMELPVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQA 1560
            HSMELPV EICEVDSQISD+SLDLDRKRKH+ SAKGNPL SAVQFIGDGVSQVQSIGNQA
Sbjct: 1501 HSMELPVPEICEVDSQISDVSLDLDRKRKHRDSAKGNPLKSAVQFIGDGVSQVQSIGNQA 1560

Query: 1561 VSNLASFLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLG 1620
            VSNLASFLNV+ DDDMNE TNTEDRVYDQIESQET+Y+ LERSSSLQSDKSSDPASMQLG
Sbjct: 1561 VSNLASFLNVTLDDDMNEHTNTEDRVYDQIESQETKYSDLERSSSLQSDKSSDPASMQLG 1620

Query: 1621 RIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVM 1680
            RIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWVVM
Sbjct: 1621 RIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVVM 1680

Query: 1681 LIYTELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLL 1740
            LIYTELY+LLQYLYQIIIQHCGL I+ D+L+ELGFPTHRITSSFVVSSLPLFLVYLFTLL
Sbjct: 1681 LIYTELYILLQYLYQIIIQHCGLTINLDVLQELGFPTHRITSSFVVSSLPLFLVYLFTLL 1740

Query: 1741 QSSITAKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWK 1800
            QSSITAKDGEWT SNA NKSALPRK S  HYGLTERAYELLYLGR MMMF+LRSLCKYWK
Sbjct: 1741 QSSITAKDGEWTSSNALNKSALPRKHSPAHYGLTERAYELLYLGRNMMMFLLRSLCKYWK 1800

Query: 1801 SLTQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSS 1860
            SLT+GAESPPYFIQVSMDVQ+WPEDGIQPERIESGINQLLQIIH +RCK +NPR CPF S
Sbjct: 1801 SLTRGAESPPYFIQVSMDVQVWPEDGIQPERIESGINQLLQIIHKDRCKVQNPRLCPFPS 1860

Query: 1861 RVHVQSIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVE 1920
            RVHVQSI RSKEN++VALVVLEVVYSSPS+D+CAEW DSLTPAADVANEIRLAQRNG VE
Sbjct: 1861 RVHVQSIERSKENSQVALVVLEVVYSSPSSDSCAEWHDSLTPAADVANEIRLAQRNGLVE 1920

Query: 1921 NTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQF 1980
            NTGFPYQI SVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNNSEFLDVYQLEDQF
Sbjct: 1921 NTGFPYQIFSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQF 1980

Query: 1981 PKEFVFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAG 2040
            PKEFVF+LMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQ+EPSNQ  G
Sbjct: 1981 PKEFVFMLMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQMEPSNQQTG 2040

Query: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100
            ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE
Sbjct: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100

Query: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGIC 2160
            LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGIC
Sbjct: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGIC 2160

Query: 2161 LFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKL 2220
            LFFILICVIW PMLMYSSGNPTNVANPIKDASCQVDIKT+SGRLTLYQTTLCEKISWDKL
Sbjct: 2161 LFFILICVIWTPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKISWDKL 2220

Query: 2221 NANVVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWI 2280
            N+N+VLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLD  QDISI FTW+
Sbjct: 2221 NSNMVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDWKQDISIDFTWV 2280

Query: 2281 LTRERPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRP 2340
            LTRERPKGKEVVRYDR V   D+P+QSDVQKVLNGS N FRIKNVYQRYFRVTGSGDVRP
Sbjct: 2281 LTRERPKGKEVVRYDRTVEAPDLPEQSDVQKVLNGSRNSFRIKNVYQRYFRVTGSGDVRP 2340

Query: 2341 LEQEDSFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTL 2400
            +EQEDS+V+ADLILNR N++WWSFHDIQPIN+S+CG FTGPVAFVISEEIPPQGILGDTL
Sbjct: 2341 MEQEDSYVTADLILNRANYKWWSFHDIQPINISECGSFTGPVAFVISEEIPPQGILGDTL 2400

Query: 2401 SKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGV 2460
            SKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGV
Sbjct: 2401 SKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGV 2460

Query: 2461 EEVLYWTLVKIYRSPHMLLEYTKID 2476
            EEVLYWTLVKIYRSPHMLLEYTKID
Sbjct: 2461 EEVLYWTLVKIYRSPHMLLEYTKID 2481

BLAST of Lcy05g006840 vs. NCBI nr
Match: XP_038899059.1 (piezo-type mechanosensitive ion channel homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 4593.5 bits (11913), Expect = 0.0e+00
Identity = 2334/2479 (94.15%), Postives = 2401/2479 (96.85%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL G LLPLLLLTAALINWSLVSLTDLLVFLFI FN HKIGFIFGRR+LVLWPIIIF
Sbjct: 1    MGSFLGGLLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRYLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            +ADI+ YRIGLVPRGDSCWVHFLSF+D LGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  SADIFWYRIGLVPRGDSCWVHFLSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFCIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKISSTSEWP+ICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSTSEWPEICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSND SS RE GETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDTSSPREEGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+VSRKMRQSLILLN VHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI L+SDVLDRE SLCREILLQLGLLGRDSMWEFLEI LLACFCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISDVLDRESSLCREILLQLGLLGRDSMWEFLEITLLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAG NKSVLLSVYTATT NY HDS SHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTATTINYCHDSHSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FVFIYCLSSFSSFRIWLSRSIDLDFYLG+NSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGFNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            YSSSDEPELLEY RLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP
Sbjct: 781  YSSSDEPELLEYGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS LS+FLGFRVF+PG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSHFLGFRVFQPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGSVLNKGKWDDPCPLFVSEEDD YDIS ++EKNK
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVSEEDD-YDISISNEKNK 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
            PSLDSGRLF+  EG DHI R SF  GQS VS+SASSKR+NSECSSS+K SFGF+WGSTKE
Sbjct: 961  PSLDSGRLFLQQEGLDHIVRPSFVSGQSQVSHSASSKRDNSECSSSRKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SHKWDK+RIISLRKERFE+QKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS
Sbjct: 1021 SHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW  PS  GVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWLMPSNTGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHL GF LSSTYRKMM
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLPGFSLSSTYRKMM 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            SQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEF+YVCRYLEAEQIGAIVREQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNN SPSPGNES R
Sbjct: 1381 AWKTEQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNVSPSPGNESFR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMELP 1500
            KR TSRIHDDAMT DIEGTLG  EQI+REDS PHPELQESLA++R  ITTESRMHSMELP
Sbjct: 1441 KRSTSRIHDDAMTTDIEGTLGNAEQIIREDSSPHPELQESLANMRIGITTESRMHSMELP 1500

Query: 1501 VIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLAS 1560
            V EICEVDSQISD+SLDLDRKRKHKGSAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLAS
Sbjct: 1501 VAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLAS 1560

Query: 1561 FLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620
            FLNV+QDDD+NEQ+N EDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI
Sbjct: 1561 FLNVTQDDDINEQSNNEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620

Query: 1621 WSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680
            W+QMR+NNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL
Sbjct: 1621 WAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680

Query: 1681 YVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740
            Y+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA
Sbjct: 1681 YILLQYLYQIIIQHCGLNINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740

Query: 1741 KDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800
            KDGEW  SNA NK+A+PR+QSLGHYGLTE+AYELLYLGRKMMMFVLRSLCKYWKSLTQGA
Sbjct: 1741 KDGEWMSSNALNKNAVPRRQSLGHYGLTEKAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800

Query: 1801 ESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQS 1860
            ESPPYFIQVS+DVQIWPEDGIQPERIESGINQ+LQIIH ERCKE+N R CPFSSRVHVQS
Sbjct: 1801 ESPPYFIQVSLDVQIWPEDGIQPERIESGINQMLQIIHDERCKEQNRRLCPFSSRVHVQS 1860

Query: 1861 IVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFPY 1920
            I RSKENTKVALVVLEVVYSSPS DTCAEWCDSLTPAADVANEIRLAQRNG VE TGFPY
Sbjct: 1861 IERSKENTKVALVVLEVVYSSPSTDTCAEWCDSLTPAADVANEIRLAQRNGLVEKTGFPY 1920

Query: 1921 QILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980
            QILSVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF
Sbjct: 1921 QILSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980

Query: 1981 ILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALRA 2040
            ILMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGELALRA
Sbjct: 1981 ILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRA 2040

Query: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100
            IFLAKAVSL LQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD
Sbjct: 2041 IFLAKAVSLTLQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100

Query: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFILI 2160
            WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQTVMTKCCNGICLFFILI
Sbjct: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILI 2160

Query: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVVL 2220
            CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSG+LTLYQTTLCEKISWDKLNAN+VL
Sbjct: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGKLTLYQTTLCEKISWDKLNANMVL 2220

Query: 2221 DPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRERP 2280
            DPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISI+FTWILTR+RP
Sbjct: 2221 DPGGYLSPYNQDDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISISFTWILTRDRP 2280

Query: 2281 KGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQEDS 2340
            KGKEVV+YDR++   D+P QSDVQKVLNGS+NGFRIKNVYQRYFRVTGSGDVRPLEQE+S
Sbjct: 2281 KGKEVVKYDRVIDPRDLPSQSDVQKVLNGSLNGFRIKNVYQRYFRVTGSGDVRPLEQEES 2340

Query: 2341 FVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400
            FVSADLILNR N++WWSFHDIQPINVS+CG FTGPVAFVISEEIPPQGILGDTLSKFSIW
Sbjct: 2341 FVSADLILNRNNYKWWSFHDIQPINVSECGSFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400

Query: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460
            GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW
Sbjct: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460

Query: 2461 TLVKIYRSPHMLLEYTKID 2476
            TLVKIYRSPHMLLEYTKID
Sbjct: 2461 TLVKIYRSPHMLLEYTKID 2478

BLAST of Lcy05g006840 vs. NCBI nr
Match: XP_008451406.1 (PREDICTED: LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel homolog [Cucumis melo])

HSP 1 Score: 4575.0 bits (11865), Expect = 0.0e+00
Identity = 2317/2479 (93.47%), Postives = 2398/2479 (96.73%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSLVSLTDLLVFLFI FN HKIGFIFGRRFLVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFL+IWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILAQVTFLLIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            TADI+ YRIGLVPRGDSCW+HF SF++ LGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFWYRIGLVPRGDSCWIHFFSFVNHLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKISS SEWP+ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSNSEWPEICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLCLSGEDE SSNDNSS  EAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSSNDNSSPGEAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDV+RKMRQSLILLNVVHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVTRKMRQSLILLNVVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI L+S+VLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPPVGFSILRAG NKSVLLSVYTATTNNY HD+PSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VEHTPSPPVGFSILRAGLNKSVLLSVYTATTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNY+SFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYLSFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            YSSSDEPELLE+ RLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP
Sbjct: 781  YSSSDEPELLEFGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS LSYFLG R F+PG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSYFLGLREFQPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGSVLNKGKWDDPCPLFV+EEDD YDIS ++EKNK
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVTEEDD-YDISISNEKNK 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
              LDSGRLF   EG DHI R SF   Q+ V +SASSKR+NSECSS++K SFGF+WGSTKE
Sbjct: 961  SPLDSGRLFEQQEGLDHIRRPSFVSAQNQVPHSASSKRDNSECSSNRKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SHKWDK+RIISLRKERFE+QKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS
Sbjct: 1021 SHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWHMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTY KMM
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYHKMM 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            SQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVS+QSYMFSSQEF+YVCRYLEAEQIGAIVREQEKK 
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSVQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKX 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNN SPSPGNES R
Sbjct: 1381 AWKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNVSPSPGNESFR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMELP 1500
             R TSRIHDDAMT DIEGTLGKVEQI+REDS PHPELQESLASLR  +TTESRMHSME P
Sbjct: 1441 MRSTSRIHDDAMTTDIEGTLGKVEQIIREDSSPHPELQESLASLRAGLTTESRMHSMESP 1500

Query: 1501 VIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLAS 1560
            V EICEVDSQISD+SLDLDRKRKHKGSAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLAS
Sbjct: 1501 VAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLAS 1560

Query: 1561 FLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620
            FLNV+QDDD+NEQ+ TED VYDQIE QETRYAHLERSSSLQSD+SSDPASMQLGRIFRHI
Sbjct: 1561 FLNVTQDDDINEQSKTEDGVYDQIEIQETRYAHLERSSSLQSDRSSDPASMQLGRIFRHI 1620

Query: 1621 WSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680
            W+QMR+NNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL
Sbjct: 1621 WAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680

Query: 1681 YVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740
            Y+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA
Sbjct: 1681 YILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740

Query: 1741 KDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800
            KDGEW YS+AFNK+ALPRKQS GHYGLT+RAYELLY+GRKMMMFVLRSLCKYWKSLTQGA
Sbjct: 1741 KDGEWAYSSAFNKNALPRKQSFGHYGLTDRAYELLYIGRKMMMFVLRSLCKYWKSLTQGA 1800

Query: 1801 ESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQS 1860
            ESPPYFIQVS+DVQIWPEDGIQPERIESGIN +LQIIHVERCKE+NPR CPFSSRVHVQS
Sbjct: 1801 ESPPYFIQVSLDVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCPFSSRVHVQS 1860

Query: 1861 IVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFPY 1920
            I RSKENTKVALVVLEVVYSSPS +TCAEWCDSLTPAADVANEIRLAQRNGFVE+TGFPY
Sbjct: 1861 IERSKENTKVALVVLEVVYSSPSTNTCAEWCDSLTPAADVANEIRLAQRNGFVESTGFPY 1920

Query: 1921 QILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980
            QILSVIGGGKREIDLYAYVFGAD+IVFF VAIFYQS+IKNNSEFLDVYQLEDQFPKEFVF
Sbjct: 1921 QILSVIGGGKREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFVF 1980

Query: 1981 ILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALRA 2040
            +LMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGELALRA
Sbjct: 1981 VLMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRA 2040

Query: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100
            IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVLD
Sbjct: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVLD 2100

Query: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFILI 2160
            WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQT+MTKCCNGICLFFILI
Sbjct: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTIMTKCCNGICLFFILI 2160

Query: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVVL 2220
            CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLN N+VL
Sbjct: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMVL 2220

Query: 2221 DPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRERP 2280
            DPGGYLSPYN++DIQLICCQADASVLWLVP VVQS FIHSLDR QDI I+FTWILTR+RP
Sbjct: 2221 DPGGYLSPYNQDDIQLICCQADASVLWLVPDVVQSGFIHSLDRKQDIIISFTWILTRDRP 2280

Query: 2281 KGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQEDS 2340
            KGKEVV+YDR++   D+P+QSDVQKVLNGSMNGFRIKNVYQRYFRVTGSG+VRPLEQE+S
Sbjct: 2281 KGKEVVKYDRVIEPRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEES 2340

Query: 2341 FVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400
            FVSADLILNR N+EWWSFHDIQPINVS+CG FTGPVAFVISEEIPPQGILGDTLSKFSIW
Sbjct: 2341 FVSADLILNRNNYEWWSFHDIQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400

Query: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460
            GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW
Sbjct: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLYW 2460

Query: 2461 TLVKIYRSPHMLLEYTKID 2476
            TLVKIYRSPHMLLEYTKID
Sbjct: 2461 TLVKIYRSPHMLLEYTKID 2478

BLAST of Lcy05g006840 vs. NCBI nr
Match: XP_011659324.1 (piezo-type mechanosensitive ion channel homolog isoform X1 [Cucumis sativus] >KAE8646323.1 hypothetical protein Csa_016796 [Cucumis sativus])

HSP 1 Score: 4539.6 bits (11773), Expect = 0.0e+00
Identity = 2305/2484 (92.79%), Postives = 2390/2484 (96.22%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSLVSLTDLLVFLFI FN HKIGFIFGRRFLVLWPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            TADI+ YRIGLVPRGDSCWVHF SF++ LGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFWYRIGLVPRGDSCWVHFFSFVNHLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKISS SEWP+ICS VSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSNSEWPEICSNVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLCLSGEDE SSNDNSS  EAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSSNDNSSPGEAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI L+S VLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI
Sbjct: 541  QISLISSVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPPVGFSILRAG NKSVLLSVYTATT+NY HD+PSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VEHTPSPPVGFSILRAGLNKSVLLSVYTATTSNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FVFIYCLSSFSSFR+WLSRSIDLDFYLG+NSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRMWLSRSIDLDFYLGFNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            YSSSDEPELLE+ RLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP
Sbjct: 781  YSSSDEPELLEFERLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGV+MT EYLFQMWG+QA MFPGQKHS LSYFLGFR F+PG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVVMTVEYLFQMWGRQAGMFPGQKHSYLSYFLGFREFQPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGS LNKGKWDDPCPLFV+EEDD YDIS ++EK+K
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSALNKGKWDDPCPLFVTEEDD-YDISISNEKSK 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
             S DSGRLF   EG DHI RSSF  GQS V +SASSKR+NSECSS+ K SFGF+WGS KE
Sbjct: 961  SSSDSGRLFEQQEGLDHIRRSSFVSGQSQVPHSASSKRDNSECSSNSKYSFGFIWGSIKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SHKWDK+RIISLRKERFE+QKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS
Sbjct: 1021 SHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWPMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+N YFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTYRKMM
Sbjct: 1141 DCWRISNQYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMM 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            SQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVS+QSYMFSSQEF+YVCRYLEAEQIGAIVREQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSIQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNS VDGNN SPSPGNES R
Sbjct: 1381 AWKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQIQLHNMNSFVDGNNVSPSPGNESFR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMELP 1500
            KR TS IHDDAMT DIEGT GKVEQI+RE+S  HPELQ+SLA+LR  +TTESRMHSMELP
Sbjct: 1441 KRSTSGIHDDAMTTDIEGTPGKVEQIIRENSSLHPELQDSLANLRAGLTTESRMHSMELP 1500

Query: 1501 VIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLAS 1560
            V EICE+D QISDLSLDLDRK+KHKGSAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLAS
Sbjct: 1501 VAEICEIDYQISDLSLDLDRKKKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLAS 1560

Query: 1561 FLNVSQDDDMNEQTNTEDRVYDQIESQETRYA-----HLERSSSLQSDKSSDPASMQLGR 1620
            FLNV  DDD NEQ+ TEDRVYDQIESQETRYA     HLERSSSLQSDKSSDPASMQLGR
Sbjct: 1561 FLNVIPDDDDNEQSKTEDRVYDQIESQETRYAHLERSHLERSSSLQSDKSSDPASMQLGR 1620

Query: 1621 IFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVML 1680
            IFRHIW+QMR+NNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVML
Sbjct: 1621 IFRHIWAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVML 1680

Query: 1681 IYTELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQ 1740
            IYTELY+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFVVSSLPLFLVYLFTLLQ
Sbjct: 1681 IYTELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQ 1740

Query: 1741 SSITAKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKS 1800
            SSITAKDGEW YS+AFNK+ALP KQSLGHYGLT+RAYELLY+GRKMM+FVLRSLCKYWKS
Sbjct: 1741 SSITAKDGEWAYSSAFNKNALPSKQSLGHYGLTDRAYELLYIGRKMMLFVLRSLCKYWKS 1800

Query: 1801 LTQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSR 1860
            LTQGAESPPYFIQVS+DVQIWPEDGIQPERIESGIN +LQIIHVERCKE+NPR C FSSR
Sbjct: 1801 LTQGAESPPYFIQVSLDVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCSFSSR 1860

Query: 1861 VHVQSIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVEN 1920
            VHVQSI RSKENTK+ALVVLEVVY+SPS +TCAEWCDSLTPAADVANEI LAQRN FVE+
Sbjct: 1861 VHVQSIERSKENTKIALVVLEVVYASPSTNTCAEWCDSLTPAADVANEILLAQRNEFVES 1920

Query: 1921 TGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFP 1980
            TGFPY+ILSVIGGGKREIDLYAYVFGAD+IVFF VAIFYQS+IKNNSEFLDVYQLEDQFP
Sbjct: 1921 TGFPYRILSVIGGGKREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFP 1980

Query: 1981 KEFVFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGE 2040
            KEFVF+LMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGE
Sbjct: 1981 KEFVFVLMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGE 2040

Query: 2041 LALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYEL 2100
            LALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTS+VSRINYLGYRLYRALPFLYEL
Sbjct: 2041 LALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYEL 2100

Query: 2101 RCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICL 2160
            RCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQTVMTKCCNGICL
Sbjct: 2101 RCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICL 2160

Query: 2161 FFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLN 2220
            FFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLN
Sbjct: 2161 FFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLN 2220

Query: 2221 ANVVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWIL 2280
             N+VLDPGGYLSPYN++DIQLICCQADASVLWLVP VVQSRF+HSLDR QDI I+FTWIL
Sbjct: 2221 TNMVLDPGGYLSPYNQDDIQLICCQADASVLWLVPDVVQSRFVHSLDRKQDIIISFTWIL 2280

Query: 2281 TRERPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPL 2340
            TR+RPKGKEVV+YDR++   D+P+QSDVQKVLNGSMNGFRIKNVYQRYFRVTGSG+VRPL
Sbjct: 2281 TRDRPKGKEVVKYDRVIESRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPL 2340

Query: 2341 EQEDSFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLS 2400
            EQE+SFVSADLILNR N+EWWSFHDIQPINVS+CG FTGPVAFVISEEIPPQGILGDTLS
Sbjct: 2341 EQEESFVSADLILNRNNYEWWSFHDIQPINVSECGRFTGPVAFVISEEIPPQGILGDTLS 2400

Query: 2401 KFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVE 2460
            KFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVE
Sbjct: 2401 KFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVE 2460

Query: 2461 EVLYWTLVKIYRSPHMLLEYTKID 2476
            EVLYWTLVKIYRSPHMLLEYTK+D
Sbjct: 2461 EVLYWTLVKIYRSPHMLLEYTKVD 2483

BLAST of Lcy05g006840 vs. NCBI nr
Match: XP_038899060.1 (piezo-type mechanosensitive ion channel homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 4503.0 bits (11678), Expect = 0.0e+00
Identity = 2289/2434 (94.04%), Postives = 2356/2434 (96.80%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL G LLPLLLLTAALINWSLVSLTDLLVFLFI FN HKIGFIFGRR+LVLWPIIIF
Sbjct: 1    MGSFLGGLLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRYLVLWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLLAI VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            +ADI+ YRIGLVPRGDSCWVHFLSF+D LGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  SADIFWYRIGLVPRGDSCWVHFLSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVAEFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFCIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKISSTSEWP+ICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSTSEWPEICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSND SS RE GETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDTSSPREEGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+VSRKMRQSLILLN VHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEVHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI L+SDVLDRE SLCREILLQLGLLGRDSMWEFLEI LLACFCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISDVLDRESSLCREILLQLGLLGRDSMWEFLEITLLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAG NKSVLLSVYTATT NY HDS SHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTATTINYCHDSHSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FVFIYCLSSFSSFRIWLSRSIDLDFYLG+NSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGFNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            YSSSDEPELLEY RLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP
Sbjct: 781  YSSSDEPELLEYGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS LS+FLGFRVF+PG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSHFLGFRVFQPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGSVLNKGKWDDPCPLFVSEEDD YDIS ++EKNK
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVSEEDD-YDISISNEKNK 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
            PSLDSGRLF+  EG DHI R SF  GQS VS+SASSKR+NSECSSS+K SFGF+WGSTKE
Sbjct: 961  PSLDSGRLFLQQEGLDHIVRPSFVSGQSQVSHSASSKRDNSECSSSRKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SHKWDK+RIISLRKERFE+QKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS
Sbjct: 1021 SHKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW  PS  GVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWLMPSNTGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHL GF LSSTYRKMM
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLPGFSLSSTYRKMM 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            SQRKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEF+YVCRYLEAEQIGAIVREQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNN SPSPGNES R
Sbjct: 1381 AWKTEQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNVSPSPGNESFR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGTLGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMELP 1500
            KR TSRIHDDAMT DIEGTLG  EQI+REDS PHPELQESLA++R  ITTESRMHSMELP
Sbjct: 1441 KRSTSRIHDDAMTTDIEGTLGNAEQIIREDSSPHPELQESLANMRIGITTESRMHSMELP 1500

Query: 1501 VIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLAS 1560
            V EICEVDSQISD+SLDLDRKRKHKGSAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLAS
Sbjct: 1501 VAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLAS 1560

Query: 1561 FLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620
            FLNV+QDDD+NEQ+N EDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI
Sbjct: 1561 FLNVTQDDDINEQSNNEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRHI 1620

Query: 1621 WSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680
            W+QMR+NNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL
Sbjct: 1621 WAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTEL 1680

Query: 1681 YVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740
            Y+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA
Sbjct: 1681 YILLQYLYQIIIQHCGLNINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSITA 1740

Query: 1741 KDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800
            KDGEW  SNA NK+A+PR+QSLGHYGLTE+AYELLYLGRKMMMFVLRSLCKYWKSLTQGA
Sbjct: 1741 KDGEWMSSNALNKNAVPRRQSLGHYGLTEKAYELLYLGRKMMMFVLRSLCKYWKSLTQGA 1800

Query: 1801 ESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQS 1860
            ESPPYFIQVS+DVQIWPEDGIQPERIESGINQ+LQIIH ERCKE+N R CPFSSRVHVQS
Sbjct: 1801 ESPPYFIQVSLDVQIWPEDGIQPERIESGINQMLQIIHDERCKEQNRRLCPFSSRVHVQS 1860

Query: 1861 IVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFPY 1920
            I RSKENTKVALVVLEVVYSSPS DTCAEWCDSLTPAADVANEIRLAQRNG VE TGFPY
Sbjct: 1861 IERSKENTKVALVVLEVVYSSPSTDTCAEWCDSLTPAADVANEIRLAQRNGLVEKTGFPY 1920

Query: 1921 QILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980
            QILSVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF
Sbjct: 1921 QILSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFVF 1980

Query: 1981 ILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALRA 2040
            ILMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQ+EPSNQHAGELALRA
Sbjct: 1981 ILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALRA 2040

Query: 2041 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100
            IFLAKAVSL LQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD
Sbjct: 2041 IFLAKAVSLTLQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2100

Query: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFILI 2160
            WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQTVMTKCCNGICLFFILI
Sbjct: 2101 WSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILI 2160

Query: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVVL 2220
            CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSG+LTLYQTTLCEKISWDKLNAN+VL
Sbjct: 2161 CVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGKLTLYQTTLCEKISWDKLNANMVL 2220

Query: 2221 DPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRERP 2280
            DPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISI+FTWILTR+RP
Sbjct: 2221 DPGGYLSPYNQDDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISISFTWILTRDRP 2280

Query: 2281 KGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQEDS 2340
            KGKEVV+YDR++   D+P QSDVQKVLNGS+NGFRIKNVYQRYFRVTGSGDVRPLEQE+S
Sbjct: 2281 KGKEVVKYDRVIDPRDLPSQSDVQKVLNGSLNGFRIKNVYQRYFRVTGSGDVRPLEQEES 2340

Query: 2341 FVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400
            FVSADLILNR N++WWSFHDIQPINVS+CG FTGPVAFVISEEIPPQGILGDTLSKFSIW
Sbjct: 2341 FVSADLILNRNNYKWWSFHDIQPINVSECGSFTGPVAFVISEEIPPQGILGDTLSKFSIW 2400

Query: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCD 2431
            GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCD
Sbjct: 2401 GLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCD 2433

BLAST of Lcy05g006840 vs. NCBI nr
Match: XP_022992489.1 (piezo-type mechanosensitive ion channel homolog [Cucurbita maxima])

HSP 1 Score: 4482.9 bits (11626), Expect = 0.0e+00
Identity = 2277/2480 (91.81%), Postives = 2366/2480 (95.40%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            MGSFL GFLLPLLLLTAALINWSL+SLTDLL+FLFI FN  KIGFIFGRRFLV WPIIIF
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVFWPIIIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            SSLVI++QVTFLVIWSLEGYKWSIANAWW KLIGFMTI+SWKSPSVIYFLVVQLL+I VA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADI+ YRIG V RGDSCWVHF SF+D LGSHLRVASCLLLPAIQIIVGIS+PSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRGDSCWVHFFSFVDHLGSHLRVASCLLLPAIQIIVGISNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+SSTSEWP ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTSEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLN+KIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNQKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC+S EDERSSNDN SAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+VSRKMRQSLILLN +HFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            QI+L+S+VLDREGSLCREILLQLGLLGRDSMWEFLEI +LA FCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEITMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAG NKSVLLSVYT TTNNY HD+PSHER+IASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERQIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVW+IGRQLVERTKRRLWFPLKAYA IV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF SFRIWLSRSIDL F+LGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            Y+SSDEPE LEY RLGF+KRFL WHSDKILFA+LFYASISPISAFGLLYLLGL +C+TLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K SHIPSKLFLAYTGVIMTAEYLFQMWGKQA MFPGQKHS+LSYFLGFRVFEPG WGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVLIIAACTLQYNVFRWLE++PGS LNKGKWDDPCPLFVS+EDD YDIS +HEKN 
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSALNKGKWDDPCPLFVSDEDD-YDISISHEKNN 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
            PSLD+GRLFV  EGSDHI R SF   QS V +SASSKR+NS CSS+ K SFGF+WGSTKE
Sbjct: 961  PSLDAGRLFVQQEGSDHILRPSFVSNQSQVPHSASSKRDNSRCSSTSKYSFGFIWGSTKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
             HKWDKMRII  RKERFEMQKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA+LNSVS
Sbjct: 1021 CHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNW-QPSKAGVHCH 1140
            LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W  PSKAGVHCH
Sbjct: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCH 1140

Query: 1141 DCWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMM 1200
            DCWRI+NLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGF LSSTYRKM+
Sbjct: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMV 1200

Query: 1201 SQRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            S RKNT VWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF
Sbjct: 1201 SHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG
Sbjct: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEF+YVCRYLEAEQIGAIV EQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLR 1440
            AWKTEQLQHIRDSEE KR+RNLQVEKMKSEMLN QIQLHNMNSS DGNNASP PGNE LR
Sbjct: 1381 AWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASPIPGNEFLR 1440

Query: 1441 KRGTSRIHDDAMTIDIEGT-LGKVEQILREDSFPHPELQESLASLRTEITTESRMHSMEL 1500
            K+ TSRI DDAMT DIEGT LGKVEQ++R DS P PELQESLA+LR    TESRMHSME 
Sbjct: 1441 KQSTSRIDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHSMEF 1500

Query: 1501 PVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNLA 1560
             V EICE+DSQISD+SLDLDRKRKHK SAKGNPL+SAVQFIGDGVSQVQSIGNQAVSNLA
Sbjct: 1501 SVPEICEIDSQISDISLDLDRKRKHKSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560

Query: 1561 SFLNVSQDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRH 1620
            SFLNV+QDDDM EQ NTEDRVYDQIESQETRY H ERSSSL SDKSSDPA+MQLGRIFRH
Sbjct: 1561 SFLNVTQDDDMIEQRNTEDRVYDQIESQETRYTHFERSSSLHSDKSSDPATMQLGRIFRH 1620

Query: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTE 1680
            IWSQMRSNNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWV+MLIYTE
Sbjct: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTE 1680

Query: 1681 LYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSIT 1740
            LY+LLQYLYQIIIQHCGL I+SDLL+ELGFPTHRITSSFV+SSLPLFLVYLFTLLQSSIT
Sbjct: 1681 LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSIT 1740

Query: 1741 AKDGEWTYSNAFNKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKSLTQG 1800
            AKDGEWTYSNAFNKS LPRK SLGHYGLTE+AYELL+LG KMMMFVLRSLCKYWKSLTQG
Sbjct: 1741 AKDGEWTYSNAFNKSDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTQG 1800

Query: 1801 AESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSRVHVQ 1860
            AESPPYFIQVSMDVQ+WPE GIQPERIESGINQ+LQIIH  RCKE+NPR CPFSSRVHVQ
Sbjct: 1801 AESPPYFIQVSMDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQ 1860

Query: 1861 SIVRSKENTKVALVVLEVVYSSPSADTCAEWCDSLTPAADVANEIRLAQRNGFVENTGFP 1920
            SI RSKEN K+ALVVLEVVYSSPS DTCAEW DSLTPAADV NEIRLAQ  G VENTGFP
Sbjct: 1861 SIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFP 1920

Query: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFV 1980
            YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNN+EFLDVYQLEDQFPKEFV
Sbjct: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFV 1980

Query: 1981 FILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAGELALR 2040
            FILMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTY VT+YAWQ+EPSNQHAGELALR
Sbjct: 1981 FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYVVTEYAWQMEPSNQHAGELALR 2040

Query: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100
            AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL
Sbjct: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100

Query: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLFFIL 2160
            DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG+KQTVMTKCCNGICLFFIL
Sbjct: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFIL 2160

Query: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNANVV 2220
            ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKT+SGRLTLYQTTLCEKI W+KLNAN+V
Sbjct: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMV 2220

Query: 2221 LDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWILTRER 2280
            LDPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLD NQDISIAFTW+LTR+R
Sbjct: 2221 LDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDR 2280

Query: 2281 PKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLEQED 2340
            PKGKEVVRYDRLV   D+P+QSD+QKVLNGSMN F+IKNVY+RYFRVTGSGDVRPLEQED
Sbjct: 2281 PKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQED 2340

Query: 2341 SFVSADLILNRENHEWWSFHDIQPINVSKCGHFTGPVAFVISEEIPPQGILGDTLSKFSI 2400
            +FVSADLILNREN++WWSFHDIQPINVSKCGHFTGPVA VISEEIPPQGILGDTLSKFSI
Sbjct: 2341 NFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSI 2400

Query: 2401 WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY 2460
            WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY
Sbjct: 2401 WGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGELGVEEVLY 2460

Query: 2461 WTLVKIYRSPHMLLEYTKID 2476
            WTLVKIYRSPHMLLEYTKID
Sbjct: 2461 WTLVKIYRSPHMLLEYTKID 2475

BLAST of Lcy05g006840 vs. TAIR 10
Match: AT2G48060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: shoot, sperm cell; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3595 (InterPro:IPR021999); Has 533 Blast hits to 341 proteins in 95 species: Archae - 2; Bacteria - 15; Metazoa - 318; Fungi - 0; Plants - 93; Viruses - 0; Other Eukaryotes - 105 (source: NCBI BLink). )

HSP 1 Score: 3275.3 bits (8491), Expect = 0.0e+00
Identity = 1687/2487 (67.83%), Postives = 2000/2487 (80.42%), Query Frame = 0

Query: 1    MGSFLRGFLLPLLLLTAALINWSLVSLTDLLVFLFILFNTHKIGFIFGRRFLVLWPIIIF 60
            M SFL GFLLP LLL AALINWS++S  DL+ FL + +   +IG+ F RR  +LWPI IF
Sbjct: 1    MASFLVGFLLPSLLLAAALINWSVISFLDLIAFLLVHYIAPEIGYRFQRRHWLLWPIFIF 60

Query: 61   SSLVIISQVTFLVIWSLEGYKWSIANAWWVKLIGFMTIESWKSPSVIYFLVVQLLAILVA 120
            S  V ++QV +LVIW+  G  W   +  W+++IGFM ++SW++P+V+YFL +QLL  LVA
Sbjct: 61   SFAVFLAQVVYLVIWAALGQDWDTPDTGWMRVIGFMILKSWRNPTVMYFLALQLLTSLVA 120

Query: 121  TADIYRYRIGLVPRGDSCWVHFLSFIDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADIY  R G     D+ W HF    + LGSHLRVASCLLLPA+Q+ VGI +PSWVSLPF
Sbjct: 121  LADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRVASCLLLPAVQLAVGICNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240
            FIGSC GLVDWSLTSN  GLFRWWR L +YAGF+I L+Y+YQLP+ +  M++W+A FIGL
Sbjct: 181  FIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNIVLLYLYQLPINFSDMIRWIASFIGL 240

Query: 241  FKISSTSEWPKICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            F+IS  +E P ICS + L+LFYIMLS V+ DLE+MDFIMS  E+NL E+LLP K+SFFIR
Sbjct: 241  FRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDMDFIMSTSENNLAERLLPPKYSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360
            E R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL ALSFWSFHFASLCAFGLLAYVGYII
Sbjct: 301  ESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLFALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF+LHRLNGLLLVFIL WA+STYIFNVAF+FLN K+GK                 
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFSFLNTKVGK----------------- 420

Query: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDNSSAREAGETKVLIVATIAWGLR 480
                  FGLG+LVAL NLVNNSVFL LS E  RSSN+ S      ETKVL+VATIAWGLR
Sbjct: 421  ------FGLGMLVALGNLVNNSVFLYLSEESSRSSNERSYVEADEETKVLVVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVSRKMRQSLILLNVVHFALLYLL 540
            K SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLSH+++RK+R+SLILL  VHFALLY+L
Sbjct: 481  KCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLSHNINRKIRKSLILLCEVHFALLYIL 540

Query: 541  QIHLVSDVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600
            +I LVS+ L +EGS  RE+L QLGLL  +S W+FLEIALLACFC IHNHGFE+LFSFSAI
Sbjct: 541  EIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFLEIALLACFCAIHNHGFEVLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGFNKSVLLSVYTATTNNYYHDSPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPP+GFSIL+AG NKSVLLSVY++ +++Y  D+ ++ER IASFLS+IG+KFLSMY
Sbjct: 601  VRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYSQDNTTYERHIASFLSAIGQKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWIIGRQLVERTKRRLWFPLKAYANIV 720
            RSCGTYIAF+TIL++V+ VKPNY+SFG++FLLL+WI GRQL E TKRRLWFPLKAYA +V
Sbjct: 661  RSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLWITGRQLFEETKRRLWFPLKAYAVLV 720

Query: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF S ++WLS  IDL FYLGYNS+A  L N W+SLAVLIVMQLYSYERRQS 
Sbjct: 721  FMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPLLDNVWESLAVLIVMQLYSYERRQSG 780

Query: 781  YSSSDEPELLEYARLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840
            +    +  LL     GF +RFL WH  KILFAALFYAS+SPIS FG +YLLGLVIC T P
Sbjct: 781  HYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALFYASLSPISVFGFVYLLGLVICTTFP 840

Query: 841  KTSHIPSKLFLAYTGVIMTAEYLFQMWGKQAEMFPGQKHSSLSYFLGFRVFEPGVWGLEL 900
            K+S IPSK FL YTG +++AEYLFQ+WG QA+MFPGQK++ LS++LG RV+EPG WG+E 
Sbjct: 841  KSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFPGQKYAELSFYLGLRVYEPGFWGIES 900

Query: 901  GLRAKVLIIAACTLQYNVFRWLEKIPGSVLNKGKWDDPCPLFVSEEDDYYDISFTHEKNK 960
            GLR KVL++AACTLQYNVFRWLE+  G  + KGK+++PCPLFVS ED    +S ++ +N 
Sbjct: 901  GLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKYEEPCPLFVSAEDTTASVSSSNGENP 960

Query: 961  PSLDSGRLFVHHEGSDHISRSSFAPGQSHVSNSASSKRENSECSSSKKSSFGFMWGSTKE 1020
             S D   + +    +   S   F+P  +  +     K   SE  SS+K SFG  WGS KE
Sbjct: 961  SSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLHPKTGGSESGSSRKFSFGHFWGSIKE 1020

Query: 1021 SHKWDKMRIISLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVS 1080
            SH+W++ RI++L+KERFE QK + KIY+KFW+EN+FNL+GLEI MI+LLLASFALLN++S
Sbjct: 1021 SHRWNRRRILALKKERFETQKNLLKIYLKFWIENMFNLYGLEINMIALLLASFALLNAIS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWQPSKAGVHCHD 1140
            + Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +EY+A W +   S+  PS+  VHCHD
Sbjct: 1081 MVYIALLAACVLLRRRVIQKLWPVVVFLFASILAIEYVATWNSFLPSDQAPSETSVHCHD 1140

Query: 1141 CWRIANLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFLLSSTYRKMMS 1200
            CW IA LYF+FC  CWLG+ VDD R L SYFVVFML+  KLRADH+S F  SSTY +M S
Sbjct: 1141 CWSIAALYFKFCRECWLGVRVDDPRTLISYFVVFMLACFKLRADHISSFSESSTYHQMKS 1200

Query: 1201 QRKNTIVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            QRKN+ VWRDLSFETKSMWT+LDYLRLYCY HLLD+VL LILITGTLEYD+LHLGYLAFA
Sbjct: 1201 QRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLLDVVLILILITGTLEYDILHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVF R+RLEILKKKNK+F+FLR YNF LII SLAYQSP VG  + GKCET+ YI+E+IGF
Sbjct: 1261 LVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLAYQSPFVGNFNDGKCETVDYIYEVIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVCRYLEAEQIGAIVREQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYV RYLEAEQIGAIVREQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVSRYLEAEQIGAIVREQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEECKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNASPSPGNESLRK 1440
             KTEQLQ IR++EE KRQRNLQVEKMKSEMLNL++QLH MNS  D N    SP  E LR+
Sbjct: 1381 RKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLRVQLHRMNS--DSNFGVASPRTEGLRR 1440

Query: 1441 RGTSRIHDD--AMTIDIEGTL-GKVEQILREDSFPHPELQESLASLRTEITTESRMHSME 1500
            R +  +  D  A + +I+G +  K EQ + EDS    E  E   S   E   +S  +S  
Sbjct: 1441 RKSPYLIPDSGAASPEIDGVVHRKEEQPIDEDSQYPFEAHEFPVSTTPE-ALDSPEYSFG 1500

Query: 1501 LPVIEICEVDSQISDLSLDLDRKRKHKGSAKGNPLISAVQFIGDGVSQVQSIGNQAVSNL 1560
                EI EV   +  +S++ +RK+K +G  K NPLISAVQ IGDGVSQVQ IGNQAV+NL
Sbjct: 1501 ASPCEITEVQQDLDVMSMERERKQKSEG--KENPLISAVQLIGDGVSQVQFIGNQAVNNL 1560

Query: 1561 ASFLNVS-QDDDMNEQTNTEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIF 1620
             +FLN+S ++ D NEQ++ +D VYD++ESQ+ ++   ERS+SLQSD+SSD  S Q+GRIF
Sbjct: 1561 VNFLNISPENSDTNEQSSVDDEVYDEMESQKRKHTPFERSTSLQSDRSSDGTSFQIGRIF 1620

Query: 1621 RHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIY 1680
            RHIWS+M+SNND+VCYCCFI+ FLWNFSLLSM YLAALF+YALCV+TGP ++FWV+ML+Y
Sbjct: 1621 RHIWSRMQSNNDIVCYCCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMY 1680

Query: 1681 TELYVLLQYLYQIIIQHCGLAIDSDLLRELGFPTHRITSSFVVSSLPLFLVYLFTLLQSS 1740
            TE+Y+LLQYLYQIIIQHCGL+ID+ LL ELGFPT RI SSFVVSSLPLFL+Y+FTL+QSS
Sbjct: 1681 TEIYILLQYLYQIIIQHCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSS 1740

Query: 1741 ITAKDGEWTYSNAF--NKSALPRKQSLGHYGLTERAYELLYLGRKMMMFVLRSLCKYWKS 1800
            IT KDG+W  S  F   ++A   ++ L    L++R  ++    R     V+RS+ +YW S
Sbjct: 1741 ITVKDGDWVPSADFTSRRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWIS 1800

Query: 1801 LTQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLQIIHVERCKEENPRSCPFSSR 1860
            LT+GAESPPYF+QV+MDV +WPEDGIQPER+E  +NQLL+++H ERC++ NP  CP+SSR
Sbjct: 1801 LTRGAESPPYFVQVTMDVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSR 1860

Query: 1861 VHVQSIVRSKENTKVALVVLEVVYSSP-SADTCAEWCDSLTPAADVANEIRLAQRNGFVE 1920
            VHVQSI RS E    ALVVLEV Y+SP +  + AEW  SLTPA+DVA EIR AQ +G  E
Sbjct: 1861 VHVQSIERSTETPNEALVVLEVEYASPTNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGE 1920

Query: 1921 NTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNSEFLDVYQLEDQF 1980
             TGFPY ILSVIGGGKR+ DLYAY+FGADLIVFF VAIFYQSVIKN SEF+DVYQLEDQF
Sbjct: 1921 GTGFPYPILSVIGGGKRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQF 1980

Query: 1981 PKEFVFILMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQIEPSNQHAG 2040
            P +FV ILM+IFFLIV+DR IYLCSFATGKV++YLF+L+LFTYAVT+YAW I P+ QHA 
Sbjct: 1981 PFDFVIILMVIFFLIVVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAA 2040

Query: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100
             LALR IFLAKA+SLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY GYRLYRALPFLYE
Sbjct: 2041 GLALRIIFLAKAMSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYE 2100

Query: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGIC 2160
            LRCVLDWSCT TSLTMYDWLKLED+NASLYLVKCD VLNR+ HK GEKQT MTKCCNGIC
Sbjct: 2101 LRCVLDWSCTATSLTMYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGIC 2160

Query: 2161 LFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKL 2220
            LFFIL+CVIWAPMLMYSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+IS D +
Sbjct: 2161 LFFILLCVIWAPMLMYSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNI 2220

Query: 2221 NANVVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISIAFTWI 2280
            +  + L    +L  YNKNDIQLICCQADASVLWLVP  V +RFI SLD + D+ I FTW+
Sbjct: 2221 DLGLDLGSQSFLPTYNKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWV 2280

Query: 2281 LTRERPKGKEVVRYDRLV---DIPDQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRP 2340
            L R+RPKGKE V+Y+R V   D+P +SD+Q VLNGSM+GFR+ N+Y ++FRVTGSGDVR 
Sbjct: 2281 LNRDRPKGKETVKYERSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRS 2340

Query: 2341 LEQEDSFVSADLILNRENHE-WWSFHDIQPI-NVSKCGHFTGPVAFVISEEIPPQGILGD 2400
             E +   VSAD+++N  N + WWSFH+++   N+S C    GPVA ++SEE PPQG LGD
Sbjct: 2341 FEDQTDEVSADILINHANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGD 2400

Query: 2401 TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDIYAARAEGEL 2460
            TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICED+YAARAEGEL
Sbjct: 2401 TLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDRLIAICEDLYAARAEGEL 2459

Query: 2461 GVEEVLYWTLVKIYRSPHMLLEYTKID 2476
            GVEEVLYWTLVKIYRSPHMLLEYTK+D
Sbjct: 2461 GVEEVLYWTLVKIYRSPHMLLEYTKLD 2459