Lcy05g005710 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g005710
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Pectinesterase
Location	Chr05: 5217511 .. 5222192 (+)
RNA-Seq Expression	Lcy05g005710
Synteny	Lcy05g005710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTTACACAGCCACATCCAAATCTCCCCCTCTGTTTCTCCTTCCTTCTTCCCCTTCTCTGTTTCAGAAATGTACAGAGCTCAAATCCCTTCAAATCCCGCCTCCATTTCCGCCATTTTCACCTTCTTCTTCATCTTCTTCCTCTTCTCTTCCAAACCCATTTCTTCGAATCCCCAAATTCACCAGACAGCTCTCACCCTCTGCAATGGCACTCTGTACCCACACCTCTGTTTCTCAAAACTTACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGCCGAGATCCACGGCGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTACGGCAGTCTCAATCCCACCGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGGCAGTATTGCTGAGCTCGAAACTTCGATTTCCGATCTGGCGCCGTCGCAGTCCGCCGCCAGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGTATTGATGGATTTTACAACAGTTCCGGGACTGTGAGGAGCCGGGTGGAGCTGTATTTGGGGAAAATTGAACAGCATTTGAGTATTGATTTGGCAATGTTGAAGAAAATCCCTGGAGTTAATCGGGCTACGGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGGTGAGGGGTGGGTTTCCGATGTGGGTTTCGGTTAAGGATCGGAGACTGTTGCAGGCGGCGGTGAATGAGACGAAGTTTGATTTGGTGGTGGCGAAGGACGGCTCCGGGAATTTCACCACCGTTGGTGAAGCAGTCGCCGCCGCTCCGAATTCGAGCACCACCAGGTTCGTTGAACTGAACTCTTTAATTTTTTTTTCTTTAATTTTATTAAAGAAAATTGTCACAATATAATTGTTTTTAACCCATATTTAAAATCAGAATTTTGAAATAAGTATTGTAATTTTTCTCTTATATGTTTAAGAGTGTAAAATTACTAATTAGATGAAAAAAGAAACACTAATTACATACATTATAGATAATTAGGTTGTAAGAGTTTGGAGATAACTCAAATTCAAATGTTATACTAATAACTAAAAGGTAGGTCTTACCCCACATTTTATGAAAAAGAAAAAATTACATTGTGACAGATTTTTCTAATAATTTCTTATTCAAACCTCATACAATTCAAGTTAAGTGGAACTTTTTAGTGTGAGCCTCTCGAAACATAAATGATTAAAGAGAAAGGAAGAAAAAAGTGATATTACTTTTTATGTAAATTTTAAGAATTAAAAAAAAAAATAGTTGAAAGATGAAGGATTAGTATGAAATAAAAACCCATAATTTTGATTAGGAAACCAGGACCAAACACCAAAAAAGACAACACTAAAAAGTTGTTCACAAAAATAAAGGCATTATTAATAAATAAGGTATTTTTTTCTTCCTAAAAAAGTAGGTATTTTATTTTTCAATAATAACAATGTACTCTTTAGAAAAAAAACAAGTTTTTTTTTTAAAAGGTTAAATTATAAATTCAAATTTTAAATTTTGAATATTACAATTATTTAGTCCTCACATAGTTATATAAAATAAAATATTATTTTTATTTTTAGTTCAATCACATGTGAACGTGTGGATTCAAACTTCTAAACTTAAATTTCAATTAAGGATCTTCATTTGACACTAAAATGTTTTTTTTTAAAATGTTAATACAAAATTCTCATTATATCATCTAATTATTTGTCACATTACATCAATTAAGATAAAATTAATCCAACAAGCAAGAAGCATAACATATTGTCAATCTGACCGCCAACATTAACATAACTCAACTTGTGTAAAACTTGTACTAACGACCAAAAGTCTGTAGTTCGAATCAGACATATATGAACGACAAATAATTAGATATTTCAATTCTCCACCATCACATACCGTTGAACTTTTAAAAGAAAAGCATATCATATTTTAATTTCATATTAACAACAAAGATTAAATAGAGATATACAATAGTATACACTACAACTACGTTGGATTAATAGAAATTGTGATTTACATATGAACAGGTTTGTAATATACATAAAAGCAGGAGCTTATTTTGAGAATGTGGAAATAGAAAGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGAAAAACGCTGATAAAGGGCAACCGGAACGTTGTCGACGGCTGGACTACTTTCCGGTCAGCCACTGTCGGTGAGTTCTCTTTTTTATTTATTAAATATTATTCAAAAAAATAAAAACCACCTGTGACCATTATCAGAATTCTCAGATCTTGCCTCTGAATTGATTTTTTTAAAAAGAATTAGTAATTTAATTAACAGTATCAATTTTGGACAGTGATTGTATCTGGGGAAGGAAGTTTTGGATCTGTTTCAATATTTTATATTTAAAAATAGATATAAAACCAAGTTCTTCAATCTCTAGTTTCTATTGCTATTAAATCTAATTGGTTAAAAGAAAAGTGATATTTTCCCTTTAATTATTTATGCATGGCATTCATTCTCACCACCTTTAGATTGAGGTTAAATGCGGAATTATAGTAAAAAATCTAAACCCTAAATTTAAGATCGAAAAAGTTAAATAAAGAAAATAAAAAATCAAAACAGAAAAGAAAAAGTAGATAGTTTTAAGATTTGAAAAGATATATATATATATATATATATTTTTTTTGAAAAGGAAAAGATATATTTTTTTTAAAAAAAATCGTACAAGATGTGGGGTTATACTTGTAGCCAGGCTGTGGATCAAGGCAGAGTACAGTGAGCATAAAGGCATCGAATTCGATCAAACCGCGTGTCGGTTGGATAAGATTGCTAATATGTCGGAAAAAAGGGTATTTTTTTCTTAGACTAACTTCTACATTCTTGATTGAAGATACATATCAATTTATCGTTAAGCTATAATCGCTTTAACAAATTATTTGTTCAACCAACATAATATTTTATACAATATGTTGATTGAACAAATAATTGATAGTTTGACATAACTTCCCATGAAAGATTAAAGTATGCCAAAACTGTGAAACTGCTTAGGCAAATGAAATACGTTTTAAATTGTGTGCTTTGGTCTTTCATCTCTTTATTTCGTTTTGGTTGTTTCTTTTTTTGACAATATGTAGAGGTGAGGATTAAACCACAGACCTCTTAATCGGGTAAAGCTTTTATTCTAGTTGAGCTATGGTCTTTCCTATCTTGTGTGACTTCATTGTGTCAAATCATTACAGCTTCTTAATGTTCAATCAATATTTCATGTCTGTATTTAAAAATAATCTTAATGAAAAATACTCGTCAAAATAAAAAAGCTAAATATATATGTTTTAGCCTCTAATGTTTGAGTGTTTTTCGATGTGGTCCATGATGTTTAGAAACGGTTACTTTAGTCCTTTATGTTTTAACATTTTTTTATTATACTCTCAAAATTTAGAAAATGTTAAAATTGACTTGTGATAAATGTACATGACACGGTACTTAGTGAGTTAAATTTGGAGGAAGAATAATTTCTATTCAAACCTTTATAAAAAAAATTGATATGAGTTTTTTTTTTACCTCCTAACATCTACAACTTTCAATCCTCAAAAATTAACTACTTATCTTCATTGTTGATTATGTTTCTCTTCATCCAGCGGCGGTTGGCAGCGGGTTCATAGCCAAAGGCATAACCTTTGAAAACTTCGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTAAGAAGCAACTCCGACTTCTCGGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCACTCCCTCCGCCAATTCTACCGTGAATGCGACGTCTACGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTCGTCTTCCAAAACTGCAACCTCTACGCCCGAAAACCCAACTCCAACCAACGCAACATATTCACCGCTCAGGGCCGTGAGGACCCCAACCAGAACACCGGAATTTCAATCCTCAATTGCAAGGTTGAAGCTGCCTCCGATTTGATCCCGGTTCTTTCCTCGTTTAAGACTTACTTGGGCCGCCCTTGGAAGTTGTACTCGAGAACGGTTTTTCTCCGATCGTTTATTGGGCAGTTGGTCGACCCGGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCGTTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCAGGTTCGAATACGACCGGACGGGTTACTTGGCCAGGTTATCGGGTGATTACTAACTCGACGGAGGCGAGTCAGTTTACGGTTGAGGCGTTTATACAAGGAACCTCGTGGTTGAACTCGACTGGGATTCCTTTCTTCTCCGGTTTGGCTTGATTGGTAAACCGGTGAGGAGGTTCTAAGATTTGGTTCTTTTTGTTTTTGTATTTTTGTAAGGATTAAGATGCTTTGATTTTGAACTGGGGAGTAAAGTGGTTGATCCACAGTCCACAAAATTAAAGGACTGTATGTGATCTTATGAAATTGCTAGTGGTTGCCATTTTAATCTTAATTATACCTAAGTTTGAGGTAGTATCTATATATGTTATTTTAGATTATGTAACTACCTACTTAAATGCTCGTCAAACGTCACTTTAATAGAGATATTTTTTGTTGAGATGATTTTTTTAGTCCTTAAACTTCAAAATGT

mRNA sequence

CTCTTACACAGCCACATCCAAATCTCCCCCTCTGTTTCTCCTTCCTTCTTCCCCTTCTCTGTTTCAGAAATGTACAGAGCTCAAATCCCTTCAAATCCCGCCTCCATTTCCGCCATTTTCACCTTCTTCTTCATCTTCTTCCTCTTCTCTTCCAAACCCATTTCTTCGAATCCCCAAATTCACCAGACAGCTCTCACCCTCTGCAATGGCACTCTGTACCCACACCTCTGTTTCTCAAAACTTACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGCCGAGATCCACGGCGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTACGGCAGTCTCAATCCCACCGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGGCAGTATTGCTGAGCTCGAAACTTCGATTTCCGATCTGGCGCCGTCGCAGTCCGCCGCCAGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGTATTGATGGATTTTACAACAGTTCCGGGACTGTGAGGAGCCGGGTGGAGCTGTATTTGGGGAAAATTGAACAGCATTTGAGTATTGATTTGGCAATGTTGAAGAAAATCCCTGGAGTTAATCGGGCTACGGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGGTGAGGGGTGGGTTTCCGATGTGGGTTTCGGTTAAGGATCGGAGACTGTTGCAGGCGGCGGTGAATGAGACGAAGTTTGATTTGGTGGTGGCGAAGGACGGCTCCGGGAATTTCACCACCGTTGGTGAAGCAGTCGCCGCCGCTCCGAATTCGAGCACCACCAGGTTTGTAATATACATAAAAGCAGGAGCTTATTTTGAGAATGTGGAAATAGAAAGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGAAAAACGCTGATAAAGGGCAACCGGAACGTTGTCGACGGCTGGACTACTTTCCGGTCAGCCACTGTCGCGGCGGTTGGCAGCGGGTTCATAGCCAAAGGCATAACCTTTGAAAACTTCGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTAAGAAGCAACTCCGACTTCTCGGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCACTCCCTCCGCCAATTCTACCGTGAATGCGACGTCTACGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTCGTCTTCCAAAACTGCAACCTCTACGCCCGAAAACCCAACTCCAACCAACGCAACATATTCACCGCTCAGGGCCGTGAGGACCCCAACCAGAACACCGGAATTTCAATCCTCAATTGCAAGGTTGAAGCTGCCTCCGATTTGATCCCGGTTCTTTCCTCGTTTAAGACTTACTTGGGCCGCCCTTGGAAGTTGTACTCGAGAACGGTTTTTCTCCGATCGTTTATTGGGCAGTTGGTCGACCCGGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCGTTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCAGGTTCGAATACGACCGGACGGGTTACTTGGCCAGGTTATCGGGTGATTACTAACTCGACGGAGGCGAGTCAGTTTACGGTTGAGGCGTTTATACAAGGAACCTCGTGGTTGAACTCGACTGGGATTCCTTTCTTCTCCGGTTTGGCTTGATTGGTAAACCGGTGAGGAGGTTCTAAGATTTGGTTCTTTTTGTTTTTGTATTTTTGTAAGGATTAAGATGCTTTGATTTTGAACTGGGGAGTAAAGTGGTTGATCCACAGTCCACAAAATTAAAGGACTGTATGTGATCTTATGAAATTGCTAGTGGTTGCCATTTTAATCTTAATTATACCTAAGTTTGAGGTAGTATCTATATATGTTATTTTAGATTATGTAACTACCTACTTAAATGCTCGTCAAACGTCACTTTAATAGAGATATTTTTTGTTGAGATGATTTTTTTAGTCCTTAAACTTCAAAATGT

Coding sequence (CDS)

ATGTACAGAGCTCAAATCCCTTCAAATCCCGCCTCCATTTCCGCCATTTTCACCTTCTTCTTCATCTTCTTCCTCTTCTCTTCCAAACCCATTTCTTCGAATCCCCAAATTCACCAGACAGCTCTCACCCTCTGCAATGGCACTCTGTACCCACACCTCTGTTTCTCAAAACTTACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGCCGAGATCCACGGCGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTACGGCAGTCTCAATCCCACCGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGGCAGTATTGCTGAGCTCGAAACTTCGATTTCCGATCTGGCGCCGTCGCAGTCCGCCGCCAGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACCAACCACCGGACTTGTATTGATGGATTTTACAACAGTTCCGGGACTGTGAGGAGCCGGGTGGAGCTGTATTTGGGGAAAATTGAACAGCATTTGAGTATTGATTTGGCAATGTTGAAGAAAATCCCTGGAGTTAATCGGGCTACGGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGGTGAGGGGTGGGTTTCCGATGTGGGTTTCGGTTAAGGATCGGAGACTGTTGCAGGCGGCGGTGAATGAGACGAAGTTTGATTTGGTGGTGGCGAAGGACGGCTCCGGGAATTTCACCACCGTTGGTGAAGCAGTCGCCGCCGCTCCGAATTCGAGCACCACCAGGTTTGTAATATACATAAAAGCAGGAGCTTATTTTGAGAATGTGGAAATAGAAAGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGAAAAACGCTGATAAAGGGCAACCGGAACGTTGTCGACGGCTGGACTACTTTCCGGTCAGCCACTGTCGCGGCGGTTGGCAGCGGGTTCATAGCCAAAGGCATAACCTTTGAAAACTTCGCCGGTCCGAGCAAGCACCAAGCCGTAGCCTTAAGAAGCAACTCCGACTTCTCGGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCACTCCCTCCGCCAATTCTACCGTGAATGCGACGTCTACGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTCGTCTTCCAAAACTGCAACCTCTACGCCCGAAAACCCAACTCCAACCAACGCAACATATTCACCGCTCAGGGCCGTGAGGACCCCAACCAGAACACCGGAATTTCAATCCTCAATTGCAAGGTTGAAGCTGCCTCCGATTTGATCCCGGTTCTTTCCTCGTTTAAGACTTACTTGGGCCGCCCTTGGAAGTTGTACTCGAGAACGGTTTTTCTCCGATCGTTTATTGGGCAGTTGGTCGACCCGGCCGGGTGGCTTGAGTGGAATGGGACGTTTGCGTTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCAGGTTCGAATACGACCGGACGGGTTACTTGGCCAGGTTATCGGGTGATTACTAACTCGACGGAGGCGAGTCAGTTTACGGTTGAGGCGTTTATACAAGGAACCTCGTGGTTGAACTCGACTGGGATTCCTTTCTTCTCCGGTTTGGCTTGA

Protein sequence

MYRAQIPSNPASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGLA

Homology

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BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.6e-155
Identity = 285/519 (54.91%), Postives = 365/519 (70.33%), Query Frame = 0


Query: 40  TALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLY 99
           T L L   T++      + TS  ++      +L  +ALN T + ++ + +N + L   L 
Sbjct: 13  TFLLLFLSTVFSSHVTQRFTSSDDIT-----ELVVTALNQTISNVNLSSSNFSDLLQRLG 72

Query: 100 GSLNPTDRRALDDCLELLDGSIAELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCI 159
            +L+  D  A DDCLELLD ++ +L T+IS L   +S +   H++  L+SAAMTN RTC+
Sbjct: 73  SNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTCL 132

Query: 160 DGFYNSSGT------------VRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAAEEVL 219
           DGF +S               V   ++  L  I  H+S  LAML+ IPG       E+V 
Sbjct: 133 DGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPGHIPGKVKEDV- 192

Query: 220 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTR 279
                   GFPMWVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TR
Sbjct: 193 --------GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETR 252

Query: 280 FVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGF 339
           FVIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK NR+  DGWT F SATV   GSGF
Sbjct: 253 FVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGF 312

Query: 340 IAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTI 399
           IAK ++F N+AGP KHQAVALRS+SD SA+Y+CSF  YQDT+YVHS +QFYRECD+YGT+
Sbjct: 313 IAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTV 372

Query: 400 DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVL 459
           DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV 
Sbjct: 373 DFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ 432

Query: 460 SSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTT 519
           ++FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T
Sbjct: 433 ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMT 492

Query: 520 GRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
            RV WPG++ I    EASQF+V  FI G  WLNST IPF
Sbjct: 493 NRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

[Close]

BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 3.3e-146
Identity = 252/319 (79.00%), Postives = 276/319 (86.52%), Query Frame = 0


Query: 232 AVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 291
           AV +   D+VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V++++KKTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 292 FVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSN 351
           F+GDGIGKT IKGNR+VVDGWTTFRS+TVA VG+GFIA+GI+FEN+AGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 352 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 411
           +DFSAFYQCSF+GYQDTLYVHSLRQFY ECDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 412 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFI 471
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLSSFKTYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 472 GQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 531
             L+DPAGWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 532 FIQGTSWLNSTGIPFFSGL 551
           FIQG+ WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

[Close]

BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-145
Identity = 268/487 (55.03%), Postives = 342/487 (70.23%), Query Frame = 0


Query: 71  KLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISD 130
           +L  + LN T  +++ A +N + L+T L+ +L   +R A +DCL LLD +I++LET++SD
Sbjct: 55  ELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 114

Query: 131 LAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--------IEQ 190
           L   +S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  
Sbjct: 115 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 174

Query: 191 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 250
           +LS  L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET ++L V
Sbjct: 175 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 234

Query: 251 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 310
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 235 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 294

Query: 311 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSF 370
           K NR+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F
Sbjct: 295 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 354

Query: 371 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 430
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 355 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 414

Query: 431 DPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 490
             +Q TGISILNC++ AA DLIPV  +FK YLGRPW+ YSRTV ++SFI  L+ PAGWLE
Sbjct: 415 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 474

Query: 491 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 550
               FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI G++WLNST
Sbjct: 475 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

[Close]

BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 502.7 bits (1293), Expect = 5.4e-141
Identity = 272/530 (51.32%), Postives = 340/530 (64.15%), Query Frame = 0


Query: 38  HQTALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTL 97
           H    ++C+ TLYP LCFS + +       S +++  ++LN T   +   Y    KL   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 98  LYGSLNPTDRRALDDCLELLDGSIAELETSISDL---APSQSAASHSHDLLTLVSAAMTN 157
             G L P +  AL DCLE +D ++ EL  ++ DL      +S   H+ DL TL+S+A+TN
Sbjct: 119 RKG-LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 158 HRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGV--------------- 217
             TC+DGF       + R  L  G+  +E   S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 218 -NRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEA 277
            N     +EV  +  +   G+P W+SV DRRLLQ +    K D  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 278 VAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFR 337
           VAAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I G+RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 338 SATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQ 397
           SATVAAVG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 398 FYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 457
           F+ +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 458 VEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGE 517
           +   SDL+ V  +F TYLGRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 518 YLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

[Close]

BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.2e-140
Identity = 269/529 (50.85%), Postives = 345/529 (65.22%), Query Frame = 0


Query: 32  SSNPQIHQTALTLCNGTLYPHLCFSKLTSFPELAS--LSPEKLASSALNFTDAEIHGAYA 91
           + N   H    + C+ T YP LCFS + + PE +    S + +   +LN T   +   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 92  NCTKLKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISDLAP---SQSAASHSHDLLT 151
              KL  L   +L   ++ AL DCLE +D ++ EL  ++ DL      +S + H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 152 LVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGVN---RAT 211
           L+SAAMTN  TC+DGF +       R  L  G+  +E+  S  LAM+K +   +     T
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 212 AAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAP 271
           +    L E  +   G+P W+S  DRRLLQ+  +    + VVA DGSGNF TV  AVAAAP
Sbjct: 240 SNNRKLTEETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAP 299

Query: 272 NSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVA 331
              T R++I IKAG Y ENVE+ +K  N+MF+GDG  +T+I G+RNVVDG TTF+SAT A
Sbjct: 300 QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 359

Query: 332 AVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYREC 391
            VG GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+  C
Sbjct: 360 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 419

Query: 392 DVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAAS 451
            + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A S
Sbjct: 420 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 479

Query: 452 DLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRG 511
           DL PV  SF TYLGRPWK YSRTV ++S I  L+ PAGW EW+G FAL+TL+YGE+ N G
Sbjct: 480 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 539

Query: 512 PGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGL 551
            G+ T+GRV W G+RVIT++TEA  FT  +FI G+SWL STG PF  GL
Sbjct: 540 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.8e-294
Identity = 506/550 (92.00%), Postives = 526/550 (95.64%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFT F + FLFS KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG LNPTDRRALDDCLELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVLSSF+TYLGRPWKLYSRT+FL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 6.5e-291
Identity = 500/550 (90.91%), Postives = 523/550 (95.09%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFTFF + FL S KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG LNPTDRRALDDCLELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK +RNVVDGWTTFRSATV AVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL SF+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 498/548 (90.88%), Postives = 518/548 (94.53%), Query Frame = 0


Query: 3   RAQIPSNPASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSFP 62
           RAQI SNP SIS I TFFFIFFLFSSKPISSNPQIHQTA +LCNGTL+P+LCFSKL+SFP
Sbjct: 7   RAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIA 122
           +LASLSPEKLA SALNFT  E+  AYANCT LKT LY  LNPTDR ALDDCLELLD SIA
Sbjct: 67  QLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLELLDDSIA 126

Query: 123 ELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+ SI DL PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRAT  EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSF 362
           KG+RNVVDGWTTFRSATVA VG+GFIAKGITFEN+AGPSKHQAVALRSNSDFSAFYQCSF
Sbjct: 307 KGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           IGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 IGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLSSF+TYLGRPWKL+SRTVFLRSFIGQLV+P GWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGSSWLNST 546

Query: 543 GIPFFSGL 551
           GIPFFSGL
Sbjct: 547 GIPFFSGL 554

[Close]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.8e-288
Identity = 497/548 (90.69%), Postives = 518/548 (94.53%), Query Frame = 0


Query: 3   RAQIPSNPASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSFP 62
           RAQI SNP SIS I TFFFIFFLFSSKPISSNPQIHQTA +LCNGTL+P+LCFSKL+SFP
Sbjct: 7   RAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIA 122
           +LASLSPEKLA SALNFT  E+  AYANCT LKT LY  LNPTDR ALDDCLELLD SIA
Sbjct: 67  QLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLELLDDSIA 126

Query: 123 ELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+ SI DL PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRAT  EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSF 362
           KG+RNVVDGWTTFRSATVA +G+GFIAKGITFEN+AGPSKHQAVALRSNSDFSAFYQCSF
Sbjct: 307 KGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           IGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 IGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLSSF+TYLGRPWKL+SRTVFLRSFIGQLV+P GWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGSSWLNST 546

Query: 543 GIPFFSGL 551
           GIPFFSGL
Sbjct: 547 GIPFFSGL 554

[Close]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 5.0e-267
Identity = 461/522 (88.31%), Postives = 485/522 (92.91%), Query Frame = 0


Query: 30  PISSNPQIHQTALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYA 89
           P+      HQTA +LCNGTL+P LCFSKL+SFP+LASLSPEKLA SALNFT  E+  AY 
Sbjct: 90  PLPLFQTFHQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYT 149

Query: 90  NCTKLKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISDLAPSQSAASHSHDLLTLVS 149
           NCT LKT LY  LNPTDR ALDDCLELLDGSIAEL+ SI DLAPSQS A HSHDLLTLVS
Sbjct: 150 NCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVS 209

Query: 150 AAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAA-EEVLP 209
           AAMTNHRTC+DGFYNSSGTVRSRVELYLGKI QHLSIDLAMLKKIPGVNRAT   +E+LP
Sbjct: 210 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLP 269

Query: 210 EYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRF 269
           EYGAV+GGFP WVSVKDRRLLQAAVNETKF++VVAKDGSGNFTTV EAVAAAPN+STTRF
Sbjct: 270 EYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRF 329

Query: 270 VIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFI 329
           VIYIKAGAYFENVEI R K+NLMFVGDGIGKTLIK +RNVVDGWTTFRSATVA VG+GFI
Sbjct: 330 VIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI 389

Query: 330 AKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTID 389
           AKGITFEN+AGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTID
Sbjct: 390 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 449

Query: 390 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 449
           FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS
Sbjct: 450 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 509

Query: 450 SFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTG 509
           SF+TYLGRPWKLYSRTVFLRSFIGQL++P GWLEWNGTFALDTLYYGEYLNRGPGSNTT 
Sbjct: 510 SFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTM 569

Query: 510 RVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGL 551
           RVTWPGYRVITN+TEASQFTVE FIQG+SWLNST IPFFSGL
Sbjct: 570 RVTWPGYRVITNATEASQFTVERFIQGSSWLNSTEIPFFSGL 611

[Close]

BLAST of Lcy05g005710 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.2e-294
Identity = 502/549 (91.44%), Postives = 526/549 (95.81%), Query Frame = 0


Query: 3   RAQIPSNPASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSFP 62
           RAQIPSNP SIS I TFFFIFF FS KPISSNPQIHQTA +LCNGTL+PHLCFSKL+SFP
Sbjct: 7   RAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQIHQTARSLCNGTLFPHLCFSKLSSFP 66

Query: 63  ELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIA 122
           +LASLSPEKLA SA+NFT  E+ GAYANCTKLKT+LYG LNPTDRRALDDCLELLD SIA
Sbjct: 67  QLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIA 126

Query: 123 ELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIEQ 182
           EL+ SI DLAP QSAA HSHDLLTL+SAAMTNHRTC+DGFYNSSGTVRSRVELYLGKI Q
Sbjct: 127 ELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQ 186

Query: 183 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 242
           HLSIDLAMLKKIPGVNRA AA EVLPEYGAV+GGFP WVSVKDRRLLQAAVNETKF+LVV
Sbjct: 187 HLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVV 246

Query: 243 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 302
           AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIER+KTNLMFVGDGIGKTLI
Sbjct: 247 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLI 306

Query: 303 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSF 362
           K +RNVVDGWTTFRSATVA VG+GFIAKGITFEN+AGPSKHQAVALRSNSDFSAFYQCSF
Sbjct: 307 KADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSF 366

Query: 363 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 422
           +GYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE
Sbjct: 367 VGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 426

Query: 423 DPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 482
           DPNQNTGISILNCKVEAASDLIPVLSSF+TYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE
Sbjct: 427 DPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 486

Query: 483 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 542
           WNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITN++EASQFTVE FIQG+SWLNST
Sbjct: 487 WNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNST 546

Query: 543 GIPFFSGLA 552
           GIPFFSGLA
Sbjct: 547 GIPFFSGLA 555

[Close]

BLAST of Lcy05g005710 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1019.6 bits (2635), Expect = 1.0e-293
Identity = 506/550 (92.00%), Postives = 526/550 (95.64%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFT F + FLFS KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG LNPTDRRALDDCLELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVLSSF+TYLGRPWKLYSRT+FL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.2e-292
Identity = 503/550 (91.45%), Postives = 525/550 (95.45%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFTFF + FL S KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG LNPTDRRALDDCLELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL SF+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.6e-291
Identity = 502/550 (91.27%), Postives = 524/550 (95.27%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFTFF +  L S KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG L+PTDRRALDDCLELLDGSI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK NRNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGT+DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDLIPVL SF+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.9e-291
Identity = 502/550 (91.27%), Postives = 523/550 (95.09%), Query Frame = 0


Query: 3   RAQIPSNP-ASISAIFTFFFIFFLFSSKPISSNPQIHQTALTLCNGTLYPHLCFSKLTSF 62
           RAQ  SNP ASISAIFTFF + FL S KPISSNPQ HQTA +LCNGTL+P+LCFSKL+SF
Sbjct: 7   RAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSF 66

Query: 63  PELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSI 122
           P+LASLSPEKLA SALNFT+ E+HGAYANC+KLKTL YG LNPTDRRALDDCLELLD SI
Sbjct: 67  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSI 126

Query: 123 AELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGKIE 182
           AEL+TSI DL+PSQSAA HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR RVELYLGKIE
Sbjct: 127 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVELYLGKIE 186

Query: 183 QHLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLV 242
           QHLSIDLAMLKKIPGV RA  AEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF+LV
Sbjct: 187 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 246

Query: 243 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 302
           VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL
Sbjct: 247 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTL 306

Query: 303 IKGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCS 362
           IK +RNVVDGWTTFRSATVAAVG+GFIAKGITFENFAGPSKHQAVA RSNSDFSAFYQCS
Sbjct: 307 IKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCS 366

Query: 363 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 422
           FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR
Sbjct: 367 FIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGR 426

Query: 423 EDPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWL 482
           EDPNQNTGISILNCKVEAASDL+PVL SF+TYLGRPWKLYSRTVFL+SFIGQLV+P GWL
Sbjct: 427 EDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWL 486

Query: 483 EWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNS 542
           EW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG+ WLNS
Sbjct: 487 EWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNS 546

Query: 543 TGIPFFSGLA 552
           T IPFFSGLA
Sbjct: 547 TEIPFFSGLA 556

[Close]

BLAST of Lcy05g005710 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 548.5 bits (1412), Expect = 6.1e-156
Identity = 285/519 (54.91%), Postives = 365/519 (70.33%), Query Frame = 0


Query: 40  TALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLY 99
           T L L   T++      + TS  ++      +L  +ALN T + ++ + +N + L   L 
Sbjct: 13  TFLLLFLSTVFSSHVTQRFTSSDDIT-----ELVVTALNQTISNVNLSSSNFSDLLQRLG 72

Query: 100 GSLNPTDRRALDDCLELLDGSIAELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCI 159
            +L+  D  A DDCLELLD ++ +L T+IS L   +S +   H++  L+SAAMTN RTC+
Sbjct: 73  SNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTCL 132

Query: 160 DGFYNSSGT------------VRSRVELYLGKIEQHLSIDLAMLKKIPGVNRATAAEEVL 219
           DGF +S               V   ++  L  I  H+S  LAML+ IPG       E+V 
Sbjct: 133 DGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPGHIPGKVKEDV- 192

Query: 220 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTR 279
                   GFPMWVS  DR LLQ  V+ETK +LVVA++G+GN+TT+GEA++AAPNSS TR
Sbjct: 193 --------GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETR 252

Query: 280 FVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGF 339
           FVIYIK G YFEN+EI R+KT +MF+GDGIG+T+IK NR+  DGWT F SATV   GSGF
Sbjct: 253 FVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGF 312

Query: 340 IAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTI 399
           IAK ++F N+AGP KHQAVALRS+SD SA+Y+CSF  YQDT+YVHS +QFYRECD+YGT+
Sbjct: 313 IAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTV 372

Query: 400 DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVL 459
           DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV 
Sbjct: 373 DFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQ 432

Query: 460 SSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTT 519
           ++FK YLGRPW+LYSRTV ++SFI  LVDPAGWL+W   FAL+TLYYGEY+N GPGSN T
Sbjct: 433 ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMT 492

Query: 520 GRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
            RV WPG++ I    EASQF+V  FI G  WLNST IPF
Sbjct: 493 NRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

[Close]

BLAST of Lcy05g005710 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 268/487 (55.03%), Postives = 342/487 (70.23%), Query Frame = 0


Query: 71  KLASSALNFTDAEIHGAYANCTKLKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISD 130
           +L  + LN T  +++ A +N + L+T L+ +L   +R A +DCL LLD +I++LET++SD
Sbjct: 55  ELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 114

Query: 131 LAPSQSAASHSHDLLTLVSAAMTNHRTCIDGFYNSSGTVRSRVELYLGK--------IEQ 190
           L   +S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  
Sbjct: 115 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 174

Query: 191 HLSIDLAMLKKIPGVNRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVV 250
           +LS  L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET ++L V
Sbjct: 175 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 234

Query: 251 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 310
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 235 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 294

Query: 311 KGNRNVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSF 370
           K NR+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F
Sbjct: 295 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 354

Query: 371 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 430
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 355 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 414

Query: 431 DPNQNTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLE 490
             +Q TGISILNC++ AA DLIPV  +FK YLGRPW+ YSRTV ++SFI  L+ PAGWLE
Sbjct: 415 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 474

Query: 491 WNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNST 550
               FAL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI G++WLNST
Sbjct: 475 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

[Close]

BLAST of Lcy05g005710 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 502.7 bits (1293), Expect = 3.8e-142
Identity = 272/530 (51.32%), Postives = 340/530 (64.15%), Query Frame = 0


Query: 38  HQTALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTL 97
           H    ++C+ TLYP LCFS + +       S +++  ++LN T   +   Y    KL   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 98  LYGSLNPTDRRALDDCLELLDGSIAELETSISDL---APSQSAASHSHDLLTLVSAAMTN 157
             G L P +  AL DCLE +D ++ EL  ++ DL      +S   H+ DL TL+S+A+TN
Sbjct: 119 RKG-LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 158 HRTCIDGFYNSSGTVRSRVELYLGK--IEQHLSIDLAMLKKIPGV--------------- 217
             TC+DGF       + R  L  G+  +E   S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 218 -NRATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEA 277
            N     +EV  +  +   G+P W+SV DRRLLQ +    K D  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 278 VAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFR 337
           VAAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I G+RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 338 SATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQ 397
           SATVAAVG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 398 FYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 457
           F+ +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 458 VEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGE 517
           +   SDL+ V  +F TYLGRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 518 YLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 547
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

[Close]

BLAST of Lcy05g005710 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 488.4 bits (1256), Expect = 7.5e-138
Identity = 258/519 (49.71%), Postives = 337/519 (64.93%), Query Frame = 0


Query: 45  CNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTKLKTLLYGSLNP 104
           C+ +LYP+LC   L  FP   +    +L   + N T  +   A    +   T+ Y  + P
Sbjct: 79  CSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TITYTQMPP 138

Query: 105 TDRRALDDCLELLDGSIAELETSISDLAPSQSAASHSHDLLTLVSAAMTNHRTCIDGF-- 164
             R A D CLELLD S+  L  ++S +       SHS D++T +S+AMTNH TC DGF  
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTCTDGFDE 198

Query: 165 -YNSSGTVRSRVELYLGKIEQHLSIDLAM----LKKIPGV----NRATAAEEVLPEYGAV 224
                G V+ +V   +  + + +S  LA+    +K + GV    NR     E   E    
Sbjct: 199 IEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE---- 258

Query: 225 RGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIK 284
               P W+  +DR LL    +  + D+ V+KDGSG F T+ EA+  AP  S+ RFVIY+K
Sbjct: 259 ---LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVK 318

Query: 285 AGAY-FENVEIERKKTNLMFVGDGIGKTLIKGNRNVVDGWTTFRSATVAAVGSGFIAKGI 344
           AG Y  EN+++ RKKTNLMF+GDG GKT+I G +++ D  TTF +AT AA G+GFI + +
Sbjct: 319 AGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDM 378

Query: 345 TFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFG 404
           TFEN+AGP+KHQAVALR   D +  Y+C+ IGYQD LYVHS RQF+REC++YGT+DFIFG
Sbjct: 379 TFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFG 438

Query: 405 NAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFKT 464
           NAAV+ Q+CN+YARKP + Q+   TAQ R+DPNQNTGISI  CK+ A  DL     S+ T
Sbjct: 439 NAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPT 498

Query: 465 YLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTW 524
           YLGRPWKLYSR V++ S +G  +DP GWLEWNG FALD+LYYGEY+N+G GS    RV W
Sbjct: 499 YLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKW 558

Query: 525 PGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPFFSGLA 552
           PGY VIT++ EAS+FTV  FI G+SWL STG+ FFSGL+
Sbjct: 559 PGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]

BLAST of Lcy05g005710 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 482.3 bits (1240), Expect = 5.4e-136
Identity = 264/544 (48.53%), Postives = 339/544 (62.32%), Query Frame = 0


Query: 34  NPQIHQTALTLCNGTLYPHLCFSKLTSFPELASLSPEKLASSALNFTDAEIHGAYANCTK 93
           +P  H    + C+ T YP LC S + +   +   S + +  +++N T   +   Y    K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 94  LKTLLYGSLNPTDRRALDDCLELLDGSIAELETSISDL---APSQSAASHSHDLLTLVSA 153
           L     G L P ++ AL DCLE +D ++ EL  ++ DL      ++   H+ DL TL+S+
Sbjct: 112 LIKKRKG-LTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISS 171

Query: 154 AMTNHRTCIDGFYNSSGTVRSRVELYLGKI--EQHLSIDLAMLK--------------KI 213
           A+TN  TC+DGF +     + R  L  G+I  E   S  LAM+K              KI
Sbjct: 172 AITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKI 231

Query: 214 PGVNR--------ATAAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFDLVVAKDG 273
              NR         T A ++         G+P W+S  DRRLLQ   +  K D  VA DG
Sbjct: 232 TSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADG 291

Query: 274 SGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKGNR 333
           SG F TV  AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N+MF+GDG  +T+I G+R
Sbjct: 292 SGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSR 351

Query: 334 NVVDGWTTFRSATVAAVGSGFIAKGITFENFAGPSKHQAVALRSNSDFSAFYQCSFIGYQ 393
           NVVDG TTF SATVAAVG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFY C  + YQ
Sbjct: 352 NVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQ 411

Query: 394 DTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQ 453
           DTLYVHS RQF+ +C + GT+DFIFGNAAVV Q+C+++AR+PNS Q+N+ TAQGR DPNQ
Sbjct: 412 DTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQ 471

Query: 454 NTGISILNCKVEAASDLIPVLSSFKTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGT 513
           NTGI I  C++ A SDL  V  SF TYLGRPWK YS+TV ++S I  ++ P GW EW GT
Sbjct: 472 NTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGT 531

Query: 514 FALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGTSWLNSTGIPF 551
           FAL+TL Y EY N G G+ T  RV W G++VIT + EA ++T   FI G  WL+STG PF
Sbjct: 532 FALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPF 591

The following BLAST results are available for this feature:

BLAST of Lcy05g005710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81301	8.6e-155	54.91	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
P85076	3.3e-146	79.00	Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
O81415	1.6e-145	55.03	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
Q42534	5.4e-141	51.32	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]
O04886	1.2e-140	50.85	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of Lcy05g005710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JU83	4.8e-294	92.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1	[more]
A0A6J1GNU4	6.5e-291	90.91	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1	[more]
A0A1S3BSU3	1.4e-288	90.88	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1	[more]
A0A5D3D1W6	1.8e-288	90.69	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130...	[more]
A0A0A0K5K8	5.0e-267	88.31	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1	[more]

BLAST of Lcy05g005710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896904.1	1.2e-294	91.44	pectinesterase-like [Benincasa hispida]	[more]
XP_022991224.1	1.0e-293	92.00	pectinesterase-like [Cucurbita maxima]	[more]
KAG6575805.1	4.2e-292	91.45	putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp...	[more]
XP_023548978.1	3.6e-291	91.27	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7014347.1	7.9e-291	91.27	putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subs...	[more]

BLAST of Lcy05g005710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02320.1	6.1e-156	54.91	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	1.1e-146	55.03	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	3.8e-142	51.32	pectin methylesterase 2	[more]
AT5G53370.1	7.5e-138	49.71	pectin methylesterase PCR fragment F	[more]
AT3G14310.1	5.4e-136	48.53	pectin methylesterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 37..190 e-value: 4.2E-26 score: 102.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..189 e-value: 1.8E-23 score: 83.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..193 e-value: 5.7E-25 score: 85.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 233..550 e-value: 3.0E-124 score: 416.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 42..196 e-value: 3.0E-28 score: 100.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 43..195
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 239..535 e-value: 2.1E-147 score: 489.6
None	No IPR available	PANTHER	PTHR31707:SF257	PECTINESTERASE	coord: 36..547
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 36..547
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 43..196 e-value: 1.35643E-34 score: 126.014
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 383..392
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 237..549

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g005710.1	Lcy05g005710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity