Homology
BLAST of Lcy05g005080 vs. ExPASy Swiss-Prot
Match:
Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)
HSP 1 Score: 411.0 bits (1055), Expect = 1.3e-113
Identity = 201/331 (60.73%), Postives = 256/331 (77.34%), Query Frame = 0
Query: 1 MPCPTAM-RTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLE 60
+P PT+M RTKKTK VGIPTIDLS R++ S+ +V+AC+++GFFKV+NH V I+ L+
Sbjct: 3 VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62
Query: 61 RQGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISN 120
+G +FF+K + EKR+AG + PFGYGCK IGPNGD G+LEYLLL S+ SI+E S +I+
Sbjct: 63 EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122
Query: 121 D-PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 180
D P+ FS IVN+Y+K V++L CEIL+L EGLW+P+KS S +I+D SDS+LRIN+YP
Sbjct: 123 DHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHYPP 182
Query: 181 VKH--TENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDP 240
VK +NW+ S + + N IGFGEHSDPQILTILRSN+V GLQIS GLW+PV PDP
Sbjct: 183 VKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDP 242
Query: 241 SAFYVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPN 300
S FYV++GDA Q +TNGRFVSVRHR + N + K RMS MYF APPLN I+PLS+M+ +
Sbjct: 243 SEFYVMVGDALQVLTNGRFVSVRHRVLTNTT-KPRMSMMYFAAPPLNWLISPLSKMVTAH 302
Query: 301 EQSLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
LY+PFTWAQYK+AAY+LRL D+RLD FK
Sbjct: 303 SPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332
BLAST of Lcy05g005080 vs. ExPASy Swiss-Prot
Match:
Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)
HSP 1 Score: 347.8 bits (891), Expect = 1.3e-94
Identity = 174/325 (53.54%), Postives = 221/325 (68.00%), Query Frame = 0
Query: 5 TAMRTKKTKPVGIPTIDLS-ESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGF 64
T +T + P ID S R++ S+ IV+AC+ GFFKVINHGV I E +G
Sbjct: 12 TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71
Query: 65 DFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSIS-NDPL 124
+FF K +K RAGPA+PFGYGCK IG NGD+G+LEYLLL ++ ++A+ S +IS +DP
Sbjct: 72 EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131
Query: 125 TFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHT 184
FS N+Y++ VR+LACEI+DL E LW S S LIRDV+SDS+LR+N+YP +
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAPYA 191
Query: 185 ENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVL 244
+ +IGFGEHSDPQILT+LRSNDVDGL+I +DGLW+P+ DP+ F+VL
Sbjct: 192 LS---------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVL 251
Query: 245 IGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYK 304
+GD QA+TNGRF SVRHR +AN +KK RMS MYF APPL A I+PL +M+ P Y
Sbjct: 252 VGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYN 311
Query: 305 PFTWAQYKKAAYSLRLADSRLDLFK 328
FTW YKKA YSLRL RL+ FK
Sbjct: 312 SFTWGDYKKATYSLRLDVPRLEFFK 327
BLAST of Lcy05g005080 vs. ExPASy Swiss-Prot
Match:
Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)
HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 167/318 (52.52%), Postives = 209/318 (65.72%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTAPEKRRA 76
IP ID+S+ R++ S IV+AC+ GFFKVINHGV I+ +E++ +FFAK A EK+
Sbjct: 15 IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74
Query: 77 GPAN-PFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISNDP-----LTFSHIVNE 136
P N PF YG + IG NGD G++EYLL +NDP L+FS VN
Sbjct: 75 RPVNQPFRYGFRDIGLNGDSGEVEYLLFH-------------TNDPAFRSQLSFSSAVNC 134
Query: 137 YVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSSK 196
Y++ V++LA EILDL EGL +P S FS LI V SDSVLR+N+YP S
Sbjct: 135 YIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSD 194
Query: 197 SHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQAM 256
S ++GFGEH+DPQILT+LRSN V GLQ+S DG+WV V PDPSAF V +GD Q M
Sbjct: 195 QSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVM 254
Query: 257 TNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEM-MLPNEQSLYKPFTWAQY 316
TNGRF+SVRHRA+ +++R+ST YF PPL A I PLS M M N+ LY+ FTW +Y
Sbjct: 255 TNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEY 314
Query: 317 KKAAYSLRLADSRLDLFK 328
KK AYSLRL DSRLD+F+
Sbjct: 315 KKRAYSLRLEDSRLDMFR 317
BLAST of Lcy05g005080 vs. ExPASy Swiss-Prot
Match:
O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)
HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 151/318 (47.48%), Postives = 214/318 (67.30%), Query Frame = 0
Query: 11 KTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTA 70
K +PV IP IDL++S A+T IV+AC++FGFFKVINHGV + LE++ +FFA
Sbjct: 21 KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80
Query: 71 PEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSI-SNDPLTFSHIVN 130
K +AGP +PFGYG K IGPNGD+G LEY+LL ++L + +T+I + P F V
Sbjct: 81 SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140
Query: 131 EYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSS 190
EY+K ++ ++ + L++V E L + K S L++ +SDS LR+N+YP + T
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEET------- 200
Query: 191 KSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQA 250
P +IGFGEH+DPQ++++LRSND +GLQI ++DG WV V PD S+F+VL+GD Q
Sbjct: 201 ---PVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQV 260
Query: 251 MTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQY 310
MTNGRF SV+HR + N+K++R+S +YF PPL+ IAPLS ++ + LY FTW+QY
Sbjct: 261 MTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQY 320
Query: 311 KKAAYSLRLADSRLDLFK 328
K +AY +L D RL LF+
Sbjct: 321 KLSAYKTKLGDYRLGLFE 325
BLAST of Lcy05g005080 vs. ExPASy Swiss-Prot
Match:
Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)
HSP 1 Score: 297.4 bits (760), Expect = 2.1e-79
Identity = 150/315 (47.62%), Postives = 207/315 (65.71%), Query Frame = 0
Query: 16 GIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTAPEKRR 75
GIP +DL+ A+ IV AC+DFGFFK++NHGV +A LE + FF K+ EK R
Sbjct: 26 GIPVVDLTHPDAK--NLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85
Query: 76 AGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSI-SNDPLTFSHIVNEYVKR 135
AGP +PFGYG K IGPNGD+G +EYLLL ++ I+ S I +P F +V Y+
Sbjct: 86 AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITA 145
Query: 136 VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSSKSHPS 195
V+ + +L+L+ EGL + ++ S L++D +SDS R+N+YP + +
Sbjct: 146 VKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL--------N 205
Query: 196 TNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQAMTNGR 255
N +GFGEH+DPQI+++LRSN GLQI L DG WV V PD ++F++ +GDA Q MTNGR
Sbjct: 206 RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGR 265
Query: 256 FVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQYKKAAY 315
F SV+HR +A+ + K+R+S +YFG P L+ IAPL +ML E+ LYK FTW +YKKAAY
Sbjct: 266 FKSVKHRVLADTT-KSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAY 325
Query: 316 SLRLADSRLDLFKAS 330
+ RLAD+RL F+ S
Sbjct: 326 TSRLADNRLAPFQKS 329
BLAST of Lcy05g005080 vs. ExPASy TrEMBL
Match:
A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)
HSP 1 Score: 587.0 bits (1512), Expect = 4.8e-164
Identity = 287/329 (87.23%), Postives = 303/329 (92.10%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ AC FGFFK+INHGVS HAI+ LER
Sbjct: 1 MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
FDFFAKTAPEK RAGPA PFGYG KAIGPNGD GDLEYLLLRSD AS+ ++S SISND
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
P TFS++VNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPAVK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180
Query: 181 HTENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFY 240
HTENWELSSKS+PS N+IGFGEHSDPQILTILRSNDVDGLQISLQDGLW PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240
Query: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSL 300
VLIGDA QAMTNGRFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300
Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
YKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Lcy05g005080 vs. ExPASy TrEMBL
Match:
A0A6J1H8N0 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460653 PE=3 SV=1)
HSP 1 Score: 580.5 bits (1495), Expect = 4.5e-162
Identity = 294/331 (88.82%), Postives = 306/331 (92.45%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC+ FG FKVINHGVS HAIA LER
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
Q FDFF +TAPEKRRAGPA PFGYGCKAIGPNGD+GDLEY+LLRSD ASIAE+S SISN
Sbjct: 61 QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP-AV 180
PL FS++VNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP AV
Sbjct: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
Query: 181 KHTE-NWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSA 240
KHTE + ELSSKSH STNKIGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
Query: 241 FYVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQ 300
FYVLIGDAFQAMTN RFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAP S+MML +Q
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Lcy05g005080 vs. ExPASy TrEMBL
Match:
A0A6J1KWQ4 (gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=3 SV=1)
HSP 1 Score: 580.1 bits (1494), Expect = 5.9e-162
Identity = 292/331 (88.22%), Postives = 304/331 (91.84%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MP PTA R+KKTK VGIPTIDLSESRARTSKSIVEAC+ FGFFKVINHGVS HAIA LER
Sbjct: 1 MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
Q FDFF +TAPEKRRAGPA PFGYGCKAIGPNGD+GDLEY+LL SD ASIAE+S SISN
Sbjct: 61 QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNS 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP-AV 180
PL FS++VNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP AV
Sbjct: 121 PLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
Query: 181 KHTE-NWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSA 240
KHTE + ELSSKSH STN IGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
Query: 241 FYVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQ 300
FY+LIGDAFQAMTN RFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAP S+MMLP +Q
Sbjct: 241 FYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300
Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
SLYKPFTWAQYKKAAYSLRLADSRL LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331
BLAST of Lcy05g005080 vs. ExPASy TrEMBL
Match:
A0A6J1JXB5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488342 PE=3 SV=1)
HSP 1 Score: 579.7 bits (1493), Expect = 7.6e-162
Identity = 284/329 (86.32%), Postives = 302/329 (91.79%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKKT+ VGIPTIDLS++R TSKSI+ AC FGFFK+INHGVS HAI+ LE
Sbjct: 1 MPCPTAMRTKKTEAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLEC 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
Q FDFFAKTAPEK RAGPA PFGYG KAIGPNGD GDLEYLLLRSD AS+ ++S SISND
Sbjct: 61 QAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
P TFS++VNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPAVK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180
Query: 181 HTENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFY 240
HTENWELSSKS+PS N+IGFGEHSDPQILTILRSNDVDGLQISLQDGLW PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240
Query: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSL 300
VLIGDA QAMTNGRFVSVRHRAMAN+SKKARMS MYFGAPPLNAWIAPLSEM+ PN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANSSKKARMSIMYFGAPPLNAWIAPLSEMIPPNHQSL 300
Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
YKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Lcy05g005080 vs. ExPASy TrEMBL
Match:
A0A6J1DEJ9 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 PE=3 SV=1)
HSP 1 Score: 578.6 bits (1490), Expect = 1.7e-161
Identity = 282/330 (85.45%), Postives = 305/330 (92.42%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKK K VGIPTIDLSESRARTSK+IVEAC++FGFFK++NHGVS AIAGLER
Sbjct: 1 MPCPTAMRTKKAKAVGIPTIDLSESRARTSKAIVEACEEFGFFKLVNHGVSDQAIAGLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
QGFDFFAK APEKRRAGPA+PFGYGCK IGPNGD G+LEYLLLRSD A+IAE+S SIS+D
Sbjct: 61 QGFDFFAKPAPEKRRAGPADPFGYGCKTIGPNGDTGELEYLLLRSDPAAIAETSLSISSD 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
P +FS+ VNEY+K V+ELACEILDLVGEGLWLPNKS+ S LIRDVQSDSVLRIN+YPAVK
Sbjct: 121 PPSFSYAVNEYIKSVKELACEILDLVGEGLWLPNKSIISGLIRDVQSDSVLRINHYPAVK 180
Query: 181 HTENWELSSKSHPSTN-KIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAF 240
HTE+WELSSKSHPS N +IGFGEHSDPQILTILRSNDV GLQISL GLWVPVCPDPSAF
Sbjct: 181 HTEDWELSSKSHPSNNTRIGFGEHSDPQILTILRSNDVGGLQISLPGGLWVPVCPDPSAF 240
Query: 241 YVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQS 300
YVLIGDAFQAM+NGRFVSVRHRAMANNS K RMS MYFGAPPLNAWIAPLSE+MLP+ S
Sbjct: 241 YVLIGDAFQAMSNGRFVSVRHRAMANNSNKPRMSMMYFGAPPLNAWIAPLSELMLPHHPS 300
Query: 301 LYKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
+Y+PFTW QYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 MYQPFTWGQYKKAAYSLRLADSRLDLFKAS 330
BLAST of Lcy05g005080 vs. NCBI nr
Match:
XP_022954040.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])
HSP 1 Score: 587.0 bits (1512), Expect = 9.9e-164
Identity = 287/329 (87.23%), Postives = 303/329 (92.10%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ AC FGFFK+INHGVS HAI+ LER
Sbjct: 1 MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
FDFFAKTAPEK RAGPA PFGYG KAIGPNGD GDLEYLLLRSD AS+ ++S SISND
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
P TFS++VNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPAVK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180
Query: 181 HTENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFY 240
HTENWELSSKS+PS N+IGFGEHSDPQILTILRSNDVDGLQISLQDGLW PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240
Query: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSL 300
VLIGDA QAMTNGRFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300
Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
YKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Lcy05g005080 vs. NCBI nr
Match:
XP_023547502.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014296.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 586.6 bits (1511), Expect = 1.3e-163
Identity = 287/329 (87.23%), Postives = 303/329 (92.10%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ AC FGFFK+INHGVS HAI+ LER
Sbjct: 1 MPCPTAMRTKKTKVVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
FDFFAKTAPEK RAGPA PFGYG KAIGPNGD GDLEYLLLRSD AS+ ++S SISND
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
P TFS++VNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPAVK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180
Query: 181 HTENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFY 240
HTENWELSSKS+PS N+IGFGEHSDPQILTILRSNDVDGLQISLQDGLW PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240
Query: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSL 300
VLIGDA QAMTNGRFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300
Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
YKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Lcy05g005080 vs. NCBI nr
Match:
KAG7025608.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 585.1 bits (1507), Expect = 3.8e-163
Identity = 295/331 (89.12%), Postives = 307/331 (92.75%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC+ FG FKVINHGVS HAIA LER
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
Q FDFF +TAPEKRRAGPA PFGYGCKAIGPNGD+GDLEY+LLRSD ASIAE+S SISN
Sbjct: 61 QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP-AV 180
PL FS++VNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP AV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
Query: 181 KHTE-NWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSA 240
KHTE + ELSSKSH STNKIGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
Query: 241 FYVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQ 300
FYVLIGDAFQAMTN RFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAP S+MMLP +Q
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300
Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Lcy05g005080 vs. NCBI nr
Match:
XP_038896324.1 (gibberellin 2-beta-dioxygenase 2 [Benincasa hispida])
HSP 1 Score: 584.7 bits (1506), Expect = 4.9e-163
Identity = 288/329 (87.54%), Postives = 301/329 (91.49%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MPCPTAMRTKKTK VGIPTIDLS+SRA TSKSIVEAC+DFGFFK+INHGVS AIA LE
Sbjct: 1 MPCPTAMRTKKTKAVGIPTIDLSQSRAITSKSIVEACQDFGFFKLINHGVSDDAIAQLES 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
QGFDFF KTA EK AGPANPFGYGCK+IGPNGDMGDLEYLLLRSD +SI + S SISN
Sbjct: 61 QGFDFFEKTALEKHCAGPANPFGYGCKSIGPNGDMGDLEYLLLRSDPSSILQCSPSISNH 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVK 180
PLTFSH+VNEYVK VR+L CEILDLV EGLWLPNKSVFSNLIRDV +DSVLRINYYP VK
Sbjct: 121 PLTFSHVVNEYVKSVRDLGCEILDLVTEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
Query: 181 HTENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFY 240
HTENW+LS KS S N+IGFGEHSDPQILTILRSNDV+GLQISLQDG+W PVCPDPSAFY
Sbjct: 181 HTENWDLSPKSCLSNNRIGFGEHSDPQILTILRSNDVNGLQISLQDGIWAPVCPDPSAFY 240
Query: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSL 300
VLIGDAFQAMTNGRFVSVRHRAMANNSK ARMS MYFGAPPLNAWIAPLSEMMLPNEQSL
Sbjct: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKNARMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
YKPFTWAQYKKAAYSLRLADSRLDLFK S
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFKTS 329
BLAST of Lcy05g005080 vs. NCBI nr
Match:
XP_023514766.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 582.0 bits (1499), Expect = 3.2e-162
Identity = 293/331 (88.52%), Postives = 306/331 (92.45%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLER 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC+ FG FKVINHGVS HAIA LER
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGIFKVINHGVSDHAIANLER 60
Query: 61 QGFDFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISND 120
Q FDFF +TAPEK RAGPA PFGYGCKAIGPNGD+GDLEY+LLRSD ASIAE+S SISN
Sbjct: 61 QAFDFFGQTAPEKHRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PLTFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP-AV 180
PL FS++VNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYP AV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
Query: 181 KHTE-NWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSA 240
KHTE + ELSSKSH STNK+GFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKVGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
Query: 241 FYVLIGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQ 300
FYVLIGDAFQAMTN RFVSVRHRAMANNSKKARMS MYFGAPPLNAWIAP S+MMLP +Q
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300
Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 330
SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Lcy05g005080 vs. TAIR 10
Match:
AT1G02400.1 (gibberellin 2-oxidase 6 )
HSP 1 Score: 347.8 bits (891), Expect = 9.4e-96
Identity = 174/325 (53.54%), Postives = 221/325 (68.00%), Query Frame = 0
Query: 5 TAMRTKKTKPVGIPTIDLS-ESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGF 64
T +T + P ID S R++ S+ IV+AC+ GFFKVINHGV I E +G
Sbjct: 12 TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71
Query: 65 DFFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSIS-NDPL 124
+FF K +K RAGPA+PFGYGCK IG NGD+G+LEYLLL ++ ++A+ S +IS +DP
Sbjct: 72 EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131
Query: 125 TFSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHT 184
FS N+Y++ VR+LACEI+DL E LW S S LIRDV+SDS+LR+N+YP +
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAPYA 191
Query: 185 ENWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVL 244
+ +IGFGEHSDPQILT+LRSNDVDGL+I +DGLW+P+ DP+ F+VL
Sbjct: 192 LS---------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVL 251
Query: 245 IGDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYK 304
+GD QA+TNGRF SVRHR +AN +KK RMS MYF APPL A I+PL +M+ P Y
Sbjct: 252 VGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYN 311
Query: 305 PFTWAQYKKAAYSLRLADSRLDLFK 328
FTW YKKA YSLRL RL+ FK
Sbjct: 312 SFTWGDYKKATYSLRLDVPRLEFFK 327
BLAST of Lcy05g005080 vs. TAIR 10
Match:
AT1G47990.1 (gibberellin 2-oxidase 4 )
HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 167/318 (52.52%), Postives = 209/318 (65.72%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTAPEKRRA 76
IP ID+S+ R++ S IV+AC+ GFFKVINHGV I+ +E++ +FFAK A EK+
Sbjct: 15 IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74
Query: 77 GPAN-PFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSISNDP-----LTFSHIVNE 136
P N PF YG + IG NGD G++EYLL +NDP L+FS VN
Sbjct: 75 RPVNQPFRYGFRDIGLNGDSGEVEYLLFH-------------TNDPAFRSQLSFSSAVNC 134
Query: 137 YVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSSK 196
Y++ V++LA EILDL EGL +P S FS LI V SDSVLR+N+YP S
Sbjct: 135 YIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSD 194
Query: 197 SHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQAM 256
S ++GFGEH+DPQILT+LRSN V GLQ+S DG+WV V PDPSAF V +GD Q M
Sbjct: 195 QSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVM 254
Query: 257 TNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEM-MLPNEQSLYKPFTWAQY 316
TNGRF+SVRHRA+ +++R+ST YF PPL A I PLS M M N+ LY+ FTW +Y
Sbjct: 255 TNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEY 314
Query: 317 KKAAYSLRLADSRLDLFK 328
KK AYSLRL DSRLD+F+
Sbjct: 315 KKRAYSLRLEDSRLDMFR 317
BLAST of Lcy05g005080 vs. TAIR 10
Match:
AT2G34555.1 (gibberellin 2-oxidase 3 )
HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 151/318 (47.48%), Postives = 214/318 (67.30%), Query Frame = 0
Query: 11 KTKPVGIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTA 70
K +PV IP IDL++S A+T IV+AC++FGFFKVINHGV + LE++ +FFA
Sbjct: 21 KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80
Query: 71 PEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSDLASIAESSTSI-SNDPLTFSHIVN 130
K +AGP +PFGYG K IGPNGD+G LEY+LL ++L + +T+I + P F V
Sbjct: 81 SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140
Query: 131 EYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSS 190
EY+K ++ ++ + L++V E L + K S L++ +SDS LR+N+YP + T
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEET------- 200
Query: 191 KSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQA 250
P +IGFGEH+DPQ++++LRSND +GLQI ++DG WV V PD S+F+VL+GD Q
Sbjct: 201 ---PVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQV 260
Query: 251 MTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQY 310
MTNGRF SV+HR + N+K++R+S +YF PPL+ IAPLS ++ + LY FTW+QY
Sbjct: 261 MTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQY 320
Query: 311 KKAAYSLRLADSRLDLFK 328
K +AY +L D RL LF+
Sbjct: 321 KLSAYKTKLGDYRLGLFE 325
BLAST of Lcy05g005080 vs. TAIR 10
Match:
AT1G30040.1 (gibberellin 2-oxidase )
HSP 1 Score: 282.0 bits (720), Expect = 6.3e-76
Identity = 145/324 (44.75%), Postives = 211/324 (65.12%), Query Frame = 0
Query: 9 TKKTKPV----GIPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFD 68
T K PV IP ++L++ A+T IV+AC++FGFFKV+NHGV + LE++
Sbjct: 19 TYKPVPVLTSHSIPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 78
Query: 69 FFAKTAPEKRRAGPANPFGYGCKAIGPNGDMGDLEYLLLRSD-LASIAESSTSISNDPLT 128
FF K RAGP P+GYG K IGPNGD+G +EYLLL ++ S ++S P
Sbjct: 79 FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQI 138
Query: 129 FSHIVNEYVKRVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTE 188
F V EY+K ++E++ ++L++V E L + + S ++RD +SDS LR+N+YPA
Sbjct: 139 FRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPA----- 198
Query: 189 NWELSSKSHPSTNKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLI 248
+ + K+GFGEH+DPQI+++LRSN+ GLQI ++DG WV V PD S+F++ +
Sbjct: 199 ----AEEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINV 258
Query: 249 GDAFQAMTNGRFVSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYKP 308
GDA Q MTNGRF SV+HR +A +++++R+S +YFG PPL+ IAPL ++ + LYK
Sbjct: 259 GDALQVMTNGRFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKE 318
Query: 309 FTWAQYKKAAYSLRLADSRLDLFK 328
FTW+QYK +AY +L D RL LF+
Sbjct: 319 FTWSQYKSSAYKSKLGDYRLGLFE 330
BLAST of Lcy05g005080 vs. TAIR 10
Match:
AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )
HSP 1 Score: 266.5 bits (680), Expect = 2.8e-71
Identity = 136/312 (43.59%), Postives = 205/312 (65.71%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACKDFGFFKVINHGVSAHAIAGLERQGFDFFAKTAPEKRRA 76
IP ID+S+ ++ ++V+AC+DFGFFKVINHGVSA ++ LE + DFF+ EK +
Sbjct: 18 IPVIDMSDPESK--HALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77
Query: 77 GPANPFGYGCKAIGPNGDMGDLEYLLLRSDLAS-IAESSTSISNDPLTFSHIVNEYVKRV 136
PFGYG IG NGD+G +EYLL+ ++ S S+ P TF + + EY V
Sbjct: 78 A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 137
Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAVKHTENWELSSKSHPST 196
R++ ++L+ + +GL + ++ S L+ D +DS+LR+N+YP + + K++
Sbjct: 138 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLS-----NKKTNGGK 197
Query: 197 NKIGFGEHSDPQILTILRSNDVDGLQISLQDGLWVPVCPDPSAFYVLIGDAFQAMTNGRF 256
N IGFGEH+DPQI+++LRSN+ GLQI+L DG W+ V PD ++F+ +GD+ Q MTNGRF
Sbjct: 198 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 257
Query: 257 VSVRHRAMANNSKKARMSTMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQYKKAAYS 316
SVRHR +A N KK+R+S +YF P L IAPL+ ++ ++ LY+ FTW++YK + Y+
Sbjct: 258 KSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYN 317
Query: 317 LRLADSRLDLFK 328
RL+D+RL F+
Sbjct: 318 SRLSDNRLQQFE 320
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9XHM5 | 1.3e-113 | 60.73 | Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1 | [more] |
Q9FZ21 | 1.3e-94 | 53.54 | Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ... | [more] |
Q9C7Z1 | 1.5e-82 | 52.52 | Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ... | [more] |
O64692 | 1.6e-79 | 47.48 | Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ... | [more] |
Q9XG83 | 2.1e-79 | 47.62 | Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GRC3 | 4.8e-164 | 87.23 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456... | [more] |
A0A6J1H8N0 | 4.5e-162 | 88.82 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460... | [more] |
A0A6J1KWQ4 | 5.9e-162 | 88.22 | gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=... | [more] |
A0A6J1JXB5 | 7.6e-162 | 86.32 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148834... | [more] |
A0A6J1DEJ9 | 1.7e-161 | 85.45 | gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_022954040.1 | 9.9e-164 | 87.23 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata] | [more] |
XP_023547502.1 | 1.3e-163 | 87.23 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1... | [more] |
KAG7025608.1 | 3.8e-163 | 89.12 | Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyros... | [more] |
XP_038896324.1 | 4.9e-163 | 87.54 | gibberellin 2-beta-dioxygenase 2 [Benincasa hispida] | [more] |
XP_023514766.1 | 3.2e-162 | 88.52 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] | [more] |