Lcy05g004510 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g004510
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	cytochrome b5 domain-containing protein RLF
Location	Chr05: 4076315 .. 4080318 (-)
RNA-Seq Expression	Lcy05g004510
Synteny	Lcy05g004510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAACCTAACTACGGTCAGCACCCCGGTCACCGTGGGCTCAAAGTTGGCCAACCACCGATTTCGTACGCAACAAACCATATTCTCCTTTCGTTTCTCTGTTGCAAATCTCGACGAGATTAGGGACCTCGGATTCCGGCCAAAAGATTCGTCGTCACCGGGCCGCCGGCGTGTCGACGGTCGATTCCGATTCAGTAAGTTGACGATTCTTTAGCTTTCTCTAAGTTTCTGAAGTTTTTTTCTTCTGGCTCACGGATTACGATTTACGATTACACAAATACACGAATCAGTTAATCTCCTTCTCAAGTTTCTCGATATTTCTTCTACCGCACCTCCATTTACTTCCTCTTCCTAATTTCAAGCTTGAATGTTAAAGACAATGTGGTATTGCCCCCCCTCTGCCTTCAATTCTGATTCTGTTTTGTCAATTGATTCAGGTAGTTTGAACTTTGATGCCTCTTGTCTCTCTGTTATGTTTTTCCTTCGTCCTGAATTCTTCACTCCTCTTCTGTGCTTTGGTTTTTCAACGAGTTCTGAATCTCAATTAGTGTGAAGCCTTTTATTTCTCTTCCGACGTCGTGATTCGATTTTAGGTTTTTTCCCCTTTCGATTTTGAATCGTGGACTACTGCTTTCTTCTGTTTCATACGATCAATCAATAGGAACAAACGATTGGACAAAATTGGATTCAGAAATACGCTTTGAATCAACCACTTGACGATGTGTTTCAACTCGTACGGTGCAAGTATGTATCGTGCGAGTTTAACATCACTCTGTTTCATTTTAGTGGTGTTAAGGTGATAAATTGGCGCAGTTCACGAATAAAAAAGCTTTACCACCATCATTCCTATGATAAATGTTAAAGTATCGATTTGGTTGCTGGATGGATGATTTTCCAGAATCTCCATAGATTACTGAATTGGTTCAGTCTTCTTAATGTGCTGATTTAAAAGTTTAAGCTTGGTCTCCTTTTCAAGAAGTGGAGTTACGGCTGATATCCTTTTTCTTCCTTGCTTGATCGAGTAAACCAGATGTAAGTTAGATTATATACATTGAATTACTGAGGACAAAAAACCATTGGTTTCAGGCTTTAACTGCTAATTTCATTATCCTCTCTTTTGTTGCTTTATAATACAGTAAAGATACTTTTTATCCCCCTTCCTATGTATTTTATATTTCGTTAAAAGTAGACTTTCACAAAAGCCAGTTAAATAAATTGCTTTTCAATTTATTAGAAATAAGCATCAAATTTTAATAGAAACTCATGTTCTATCTACAATCTGTTGACTTCTGGAATTGCAATGCAGCAGAACAATTTCTTGGAGTTCTGTTGTTGGATGAGAGAGGTTGCTTGAAATTTGAGACTGAATAGACTGAGATGGACAGTGACAACGACTTCACATTTTGTCAGGCAAGTATAGTTTCTATGCTATAAAATTAGGTTTTAAGTTTTCAACTCAATGGCATGTTTACTTCTTTCTATTCTTTTACCTTCTACAGTTGGAATAATGTTTGATATCCAACCTAAGTCATAGTACTGTTGCTGTTATACGAATATATTAGTAGGTGGGTTTACCTGAAAAAGATGATAAAGAGGCGAAGGATCTTGCATCAGATATAGGTGATATTACCTTAAGGGATGAATTGACGAATGGCACTGTTAGTAGGACGCATGTCGTTTGGAATGATAAATTGTCAAATGATATAACTTCCAGAAAGCAGGCAACAGTGGGTTCCCTGCATTTCAATGTTATTGATGTGTCATCTTCAAAACAAGAAGAGGTGTCGAAAGATGCTGGAAAGGTTGTGAAGAATTTAGGAAAACAAGGGACTGCTGCACGTAAGCCTGCAACACGAACGAAGGTTCCTTTTGAAAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACCCATCCTGATCTTGCAGGTATGTGTGGAAATGGTATCCAGTGTAACTGCCTTGCATTTTGATATTATGATTGGCTAAATTATAAAAAATGCCCCTGAACTATGGGATTTGTTTAAAAAATGTCCTGAACTTTAAAAAGTTTCAAAAATACCCTTGAACTTTAAAAAATGTTTCAAAAATACCCATGTCGTTAATTTTTGGATGGAAACCATTAAAAATTTCAGTTTCATTAATACTCTTTAACTTTTAAAAGATTTTAAAAAATAACCTTATTAGAAAATGCTTCTTTTTTTTTTCCTTATCCCACCGTAAATTCTTCCTCCATGAAAATTAATGAGAAGCTTTACCTATTTTTCATGTATTCATACACTAGGAGTTTTTCTTCTCTTTGAACACATCATCCCAAGAGCTTGACTAGATTTTCGATAATGGTATTTTCAAAACATTTTTAACGGTTCCCATCCAAAAATTAACTGCAAAGGTATTTTTGAAACGTTTTGTAAAGTTCAGGGGTATTTTTGAAACTTTTTAAAGTTCAAGGACATTTTTGAAACAAACTCCATAGTTCAGGGGTATTTTTCATAATTTAGCCATTATGATTTGCTTGAGCCTATATGGCAACTCTTTTATTCTTCTTGTTAAGACGATTTTGTATCTTTTAATTTGGGTTAAAAAAACGCTTATGGTCCTTGAACTTTCATGAAAATAACCATTTTTTTTTAAATCTTAGTTTGTAACAAGTCCATGTACTATCAAATTTGTAAGAATTTAGTCATTGAACTTTAGTATGTAATAATTTTGTATTATACTTTCAAATTTATAACAATTTAATCCAAGCCGTGAAAAATTGCATCAAAATTAAGTGTCAATTTTTATGATGTAACGAATTAGTCTTTGTAGTTTATAAACACATTTGGTTTCTAATCAATTTATCGATCTACATATGTTAGAAATTCCATTAAATCTCAACAATATTTTTGATGATAGGGACTAAATTGTTACAAAATTGAAAGTGTAGGGATGAGATCGTTAGAAACTAACATTTAGGGACAGAGTCTTTTTTAACCTTAATTTTATGACCTGGCACCTTGGCTTATGTTTATGTTTGTTTGGGCTATTAAAGAGATATAAATTGTTTGCAGCTAGATGATGCTTGGCATGTTCAGGATTCAGGAAGACGCTAAAAGATCTATTTTATCGAAATTATGAACTTCACAGAGGACCCACTTGCCATTTTCGTTGTCTATAACAGTACATTATTATTATGATAGTTATTGTTTGTGTTGCAAAATAGGACCATTTTATTGTTTTCATGATTACTGGTAGTTTAATGTTATTAATTTCCGCACTGAGAAATAATGCAATAGATTTTCAACATTTGTTTCATAATTGTGGATTAGGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCTGGAGGTATGGGCATCATGCAAAAATTTTCTATGTTTAAATTTTCTTTTAGTGAATTTATTATCCATTCCATCCTGCTATAATGAGCTTGCCTCACTCAGAGGACTCTAGAGTTGGTAAAGTAGCATGATTAGTTTGTAATGTATGTTATGGTGTCTCGTGTTCAAGAATTGCCCTTCCAGTCTATAGCTTCTTCTATATTTTATCTTTAAGACAGCATTCAAAGTTCAATTTACATGAAATTGACTACAGTATCATGTTATTGAATTCCGTGAAGGATGATTATTCTCCTTTATTCGTGACCTAGGTAGGAAGCTTAGCTCATGAATACTGGCTTCTAGTTCTACTTATAGATTGCTGGTTGATTTTCAGATGTACTTTCTAGTTATTATTTGAGACGATTCTTGTATGTTTTAAATGATGGGTGAGTTTTATTGGAGTACATCATAGGCATTTTCTCGGGGGTAGACTTGGACTACTTGACATTTTGACAAACTTCTTTTATCAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCA

mRNA sequence

ATGGTCAACCTAACTACGGTCAGCACCCCGGTCACCGTGGGCTCAAAGTTGGCCAACCACCGATTTCGTACGCAACAAACCATATTCTCCTTTCGTTTCTCTGTTGCAAATCTCGACGAGATTAGGGACCTCGGATTCCGGCCAAAAGATTCGTCGTCACCGGGCCGCCGGCGTGTCGACGGTCGATTCCGATTCAGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCTGGAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCA

Coding sequence (CDS)

ATGGTCAACCTAACTACGGTCAGCACCCCGGTCACCGTGGGCTCAAAGTTGGCCAACCACCGATTTCGTACGCAACAAACCATATTCTCCTTTCGTTTCTCTGTTGCAAATCTCGACGAGATTAGGGACCTCGGATTCCGGCCAAAAGATTCGTCGTCACCGGGCCGCCGGCGTGTCGACGGTCGATTCCGATTCAGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCTGGAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCA

Protein sequence

MVNLTTVSTPVTVGSKLANHRFRTQQTIFSFRFSVANLDEIRDLGFRPKDSSSPGRRRVDGRFRFRLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF

Homology

[Close]

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot
Match: Q9LXD1 (Cytochrome b5 domain-containing protein RLF OS=Arabidopsis thaliana OX=3702 GN=RLF PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.4e-22
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKG+SNKRLIPM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T L
Sbjct: 120 LKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLL 179

Query: 127 F 128
           F
Sbjct: 180 F 180

[Close]

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot
Match: Q28CZ9 (Cytochrome b5 reductase 4 OS=Xenopus tropicalis OX=8364 GN=cyb5r4 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.1e-10
Identity = 38/90 (42.22%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 47  RPKDSSSPGRRRVD-------GRFRFRLKGQSNKRLIPMS--EVKQHQMEGSMWTVLKGR 106
           R K    PGR  +D       G+    LKG    RLI ++  E+ QH  +   W  ++G 
Sbjct: 23  RSKVPLKPGRSLMDWIRLTKSGKDLTGLKG----RLIDVTEEELAQHNKKEDCWICIRGM 82

Query: 107 VYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           VYNI+PYM++HPGG + LMKA G+D T LF
Sbjct: 83  VYNITPYMEYHPGGEEELMKAAGRDGTDLF 108

[Close]

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot
Match: Q68EJ0 (Cytochrome b5 reductase 4 OS=Rattus norvegicus OX=10116 GN=Cyb5r4 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-09
Identity = 25/48 (52.08%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 80  EVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           E+K+H  +   W  ++G VYN+SPYM++HPGG D LM+A G D T LF
Sbjct: 61  ELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLF 108

[Close]

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot
Match: Q32LH7 (Cytochrome b5 reductase 4 OS=Bos taurus OX=9913 GN=CYB5R4 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 38/92 (41.30%), Postives = 51/92 (55.43%), Query Frame = 0


Query: 45  GFRPKDSSSPGRRRVD-------GRFRFRLKGQSNKRLIPMS--EVKQHQMEGSMWTVLK 104
           G R K     GR  +D       G+    LKG    RLI ++  E+K+H  +   W  ++
Sbjct: 21  GGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIR 80

Query: 105 GRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           G VYN+SPYM++HPGG D LM+A G D T LF
Sbjct: 81  GFVYNVSPYMEYHPGGEDELMRAAGSDGTDLF 108

[Close]

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot
Match: Q7L1T6 (Cytochrome b5 reductase 4 OS=Homo sapiens OX=9606 GN=CYB5R4 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 38/92 (41.30%), Postives = 51/92 (55.43%), Query Frame = 0


Query: 45  GFRPKDSSSPGRRRVD-------GRFRFRLKGQSNKRLIPMS--EVKQHQMEGSMWTVLK 104
           G R K     GR  +D       G+    LKG    RLI ++  E+K+H  +   W  ++
Sbjct: 21  GGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIR 80

Query: 105 GRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           G VYN+SPYM++HPGG D LM+A G D T LF
Sbjct: 81  GFVYNVSPYMEYHPGGEDELMRAAGSDGTELF 108

[Close]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL
Match: A0A6J1D8C4 (cytochrome b5 domain-containing protein RLF OS=Momordica charantia OX=3673 GN=LOC111017852 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-26
Identity = 60/61 (98.36%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVKQHQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 145 LKGQSNKRLIPMSEVKQHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 204

Query: 127 F 128
           F
Sbjct: 205 F 205

[Close]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL
Match: A0A6J1H7H2 (cytochrome b5 domain-containing protein RLF-like OS=Cucurbita moschata OX=3662 GN=LOC111461137 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.4e-26
Identity = 59/61 (96.72%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 144 LKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 203

Query: 127 F 128
           F
Sbjct: 204 F 204

[Close]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.8e-26
Identity = 58/61 (95.08%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+H+ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 141 LKGQSNKRLIPMSEVKEHRTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 200

Query: 127 F 128
           F
Sbjct: 201 F 201

[Close]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL
Match: A0A6J1KSP4 (cytochrome b5 domain-containing protein RLF OS=Cucurbita maxima OX=3661 GN=LOC111497877 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 8.9e-26
Identity = 57/61 (93.44%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYN+SPYMKFHPGGADILMKAVG+DCTSL
Sbjct: 144 LKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNLSPYMKFHPGGADILMKAVGRDCTSL 203

Query: 127 F 128
           F
Sbjct: 204 F 204

[Close]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL
Match: A0A0A0K899 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426500 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.6e-25
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+H+ EGSMWTVLKG VYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 229 LKGQSNKRLIPMSEVKEHRTEGSMWTVLKGHVYNISPYMKFHPGGADILMKAVGKDCTSL 288

Query: 127 F 128
           F
Sbjct: 289 F 289

[Close]

BLAST of Lcy05g004510 vs. NCBI nr
Match: KAG7025640.1 (Cytochrome b5 domain-containing protein RLF, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 134.0 bits (336), Expect = 8.8e-28
Identity = 69/110 (62.73%), Postives = 78/110 (70.91%), Query Frame = 0


Query: 23  RTQQTIFSFRFSVANLDEIRDLGFR-----PKDSSSPGRRRVDGRFRFRLKGQSNKRLIP 82
           R Q T+ S  F+V ++   +    +     PKD+    +         RLKGQSNKRLIP
Sbjct: 90  RKQATVGSLHFNVTDMSSSKQSSTQSTEKVPKDAEKVAKVSGKQGTAGRLKGQSNKRLIP 149

Query: 83  MSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           MSEVK+HQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF
Sbjct: 150 MSEVKEHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 199

[Close]

BLAST of Lcy05g004510 vs. NCBI nr
Match: XP_022149422.1 (cytochrome b5 domain-containing protein RLF [Momordica charantia] >XP_022149431.1 cytochrome b5 domain-containing protein RLF [Momordica charantia])

HSP 1 Score: 129.4 bits (324), Expect = 2.2e-26
Identity = 60/61 (98.36%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVKQHQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 145 LKGQSNKRLIPMSEVKQHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 204

Query: 127 F 128
           F
Sbjct: 205 F 205

[Close]

BLAST of Lcy05g004510 vs. NCBI nr
Match: KAG6593288.1 (Cytochrome b5 domain-containing protein RLF, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 128.3 bits (321), Expect = 4.9e-26
Identity = 59/61 (96.72%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 144 LKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 203

Query: 127 F 128
           F
Sbjct: 204 F 204

[Close]

BLAST of Lcy05g004510 vs. NCBI nr
Match: XP_022960400.1 (cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata] >XP_022960401.1 cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata])

HSP 1 Score: 128.3 bits (321), Expect = 4.9e-26
Identity = 59/61 (96.72%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 144 LKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 203

Query: 127 F 128
           F
Sbjct: 204 F 204

[Close]

BLAST of Lcy05g004510 vs. NCBI nr
Match: XP_023515005.1 (cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo] >XP_023515006.1 cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.5 bits (319), Expect = 8.3e-26
Identity = 58/61 (95.08%), Postives = 60/61 (98.36%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYN+SPYMKFHPGGADILMKAVGKDCTSL
Sbjct: 144 LKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNLSPYMKFHPGGADILMKAVGKDCTSL 203

Query: 127 F 128
           F
Sbjct: 204 F 204

[Close]

BLAST of Lcy05g004510 vs. TAIR 10
Match: AT5G09680.1 (reduced lateral root formation )

HSP 1 Score: 105.5 bits (262), Expect = 3.1e-23
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKG+SNKRLIPM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T L
Sbjct: 120 LKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLL 179

Query: 127 F 128
           F
Sbjct: 180 F 180

[Close]

BLAST of Lcy05g004510 vs. TAIR 10
Match: AT5G09680.2 (reduced lateral root formation )

HSP 1 Score: 105.5 bits (262), Expect = 3.1e-23
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 67  LKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSL 126
           LKG+SNKRLIPM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T L
Sbjct: 120 LKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLL 179

Query: 127 F 128
           F
Sbjct: 180 F 180

[Close]

BLAST of Lcy05g004510 vs. TAIR 10
Match: AT3G61580.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 60.8 bits (146), Expect = 8.9e-10
Identity = 21/58 (36.21%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 70  QSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           ++ K+ I   ++K+H   G +W  ++G+VYN+S ++K HPGG  +++  VG+D T  F
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61

[Close]

BLAST of Lcy05g004510 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 21/54 (38.89%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 74  RLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           ++  +SEV +H      W V+ G+VYN++ +++ HPGG D+L+ + GKD T  F
Sbjct: 6   KIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDF 59

[Close]

BLAST of Lcy05g004510 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 20/57 (35.09%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 71  SNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLF 128
           S+++++   EV +H      W ++ G+VY+++P+M  HPGG ++L+ + GKD T+ F
Sbjct: 3   SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDF 59

The following BLAST results are available for this feature:

BLAST of Lcy05g004510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXD1	4.4e-22	78.69	Cytochrome b5 domain-containing protein RLF OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q28CZ9	5.1e-10	42.22	Cytochrome b5 reductase 4 OS=Xenopus tropicalis OX=8364 GN=cyb5r4 PE=2 SV=1	[more]
Q68EJ0	1.1e-09	52.08	Cytochrome b5 reductase 4 OS=Rattus norvegicus OX=10116 GN=Cyb5r4 PE=1 SV=2	[more]
Q32LH7	1.5e-09	41.30	Cytochrome b5 reductase 4 OS=Bos taurus OX=9913 GN=CYB5R4 PE=2 SV=1	[more]
Q7L1T6	1.5e-09	41.30	Cytochrome b5 reductase 4 OS=Homo sapiens OX=9606 GN=CYB5R4 PE=1 SV=1	[more]

BLAST of Lcy05g004510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8C4	1.1e-26	98.36	cytochrome b5 domain-containing protein RLF OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1H7H2	2.4e-26	96.72	cytochrome b5 domain-containing protein RLF-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A1S3CEH4	6.8e-26	95.08	disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=...	[more]
A0A6J1KSP4	8.9e-26	93.44	cytochrome b5 domain-containing protein RLF OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0K899	2.6e-25	93.44	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Lcy05g004510 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025640.1	8.8e-28	62.73	Cytochrome b5 domain-containing protein RLF, partial [Cucurbita argyrosperma sub...	[more]
XP_022149422.1	2.2e-26	98.36	cytochrome b5 domain-containing protein RLF [Momordica charantia] >XP_022149431....	[more]
KAG6593288.1	4.9e-26	96.72	Cytochrome b5 domain-containing protein RLF, partial [Cucurbita argyrosperma sub...	[more]
XP_022960400.1	4.9e-26	96.72	cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata] >XP_022960...	[more]
XP_023515005.1	8.3e-26	95.08	cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo] >X...	[more]

BLAST of Lcy05g004510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09680.1	3.1e-23	78.69	reduced lateral root formation	[more]
AT5G09680.2	3.1e-23	78.69	reduced lateral root formation	[more]
AT3G61580.1	8.9e-10	36.21	Fatty acid/sphingolipid desaturase	[more]
AT2G32720.1	2.0e-09	38.89	cytochrome B5 isoform B	[more]
AT5G53560.1	2.0e-09	35.09	cytochrome B5 isoform E	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 76..127 e-value: 7.2E-6 score: 35.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 78..127 e-value: 4.5E-16 score: 58.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 73..127 score: 18.8561
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 71..127 e-value: 3.1E-19 score: 70.8
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 72..127
None	No IPR available	PANTHER	PTHR43112	FERREDOXIN	coord: 67..127
None	No IPR available	PANTHER	PTHR43112:SF5	CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN RLF	coord: 67..127
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 104..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g004510.1	Lcy05g004510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
cellular_component	GO:0005829	cytosol
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding