Lcy05g003770 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g003770 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 5.9e-233
Identity = 399/543 (73.48%), Postives = 461/543 (84.90%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           M  +T Q  + IFRSKLPDI+IPNHLPLH Y F+  ++F SRPCLINGA   +Y+Y DV+
Sbjct: 1   MEKDTKQV-DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SR+VA GLH  GI+  D IM LLPNSPEFVF F+ ASY GAI T ANP +TP E+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFA 180
           AKA++ K+IVT AC  ++VKD A EN VKI+C+D A EGCLHFSVL+ A+E   P V+  
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQ 180

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY H +DV+LCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IVS L+L+++Y+V++ P VPPI LAIAKSP  + YD+
Sbjct: 241 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 300

Query: 301 SSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELED VRAKFP A LGQGYGMTEAGPVLAM LAFAKEPF +K+GA
Sbjct: 301 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 360

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N +GEICIRGDQIMKGYLND E+T RT+DKEGWL+TG
Sbjct: 361 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 420

Query: 421 DIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVP 480
           DIG+ID+DDELFIVDRLKELIK+K FQVAPAELEALLL HP +SDAAV+ M DEQAGEVP
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG TITE++VK FISKQV+FYKR+KRVFFV+AIPK+PSGKILRK+LRAKLA+G
Sbjct: 481 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 LPN 542

BLAST of Lcy05g003770 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 5.0e-232
Identity = 393/543 (72.38%), Postives = 455/543 (83.79%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           M  ETNQ  + IFRSKLPDI+IP HLPLH Y F+  ++F SRPCLINGA  H+Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LTSR+VA GL+ LGI++ D IM LLPNSPEFVF F+ ASY GAI T ANP +TP E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANVKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFA 180
           AKA+N KLI+T ACF ++VKD A +N + ++C+D A EGC+HFS L+ ADE   P V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIY 240
           +DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY H +DVL+CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IV   +L+EKY+V++ P VPPI LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVLAM LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RT+DKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVP 480
           DIG+ID DDELFIVDRLKELIK+K FQVAPAELEALLL HP +SDAAV+ M DEQAGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  ITE++VK F+SKQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 VPN 544

BLAST of Lcy05g003770 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-231
Identity = 392/540 (72.59%), Postives = 453/540 (83.89%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQLTS 63
           ET Q+ + IFRSKLPDI+IP HLPLH Y F+  ++F SRPCLINGA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQAKA 123
           R+VA GL+ LGI++ D IM LLPNSPEFVF F+ ASY GAI T ANP +TP E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANVKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAADD 183
           ++ K+I+T +CF  +VKD A EN VK++C+D A EGCLHFS L+ +DE   P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY H +DVL+CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVSSV 303
           SILLCGLR GAAILIMQKF+I   L+L++KY+VS+ P VPPI LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVLAM LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPVAF 483
           FIDEDDELFIVDRLKELIK+K FQVAPAE+EALLL HP +SDAAV+ M DEQAGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
           VV++NG  ITE++VK FISKQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Lcy05g003770 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.0e-229
Identity = 388/543 (71.45%), Postives = 453/543 (83.43%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           M +ET Q+ + IFRSKLPDI+IP HLPLH Y F+  ++F SRPCLI+GA   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LTSR+VA GL+ LGI++ D IM LLPN PEFVF F+ ASY GAI T ANP +TP E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANVKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFA 180
           AKA++ K+++T ACF  +VKD A EN +K++CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG N NLY H DDVL+CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LR GAAILIMQKF+I   L+L+ K++V++ P VPPI LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVLAM LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVP 480
           DIGFID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG TITE++VK FISKQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Lcy05g003770 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.2e-229
Identity = 398/546 (72.89%), Postives = 457/546 (83.70%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           +A+E  Q  + IFRSKLPDI+IP +LPLH Y F+  +KF SRPCLINGAT  +++Y DV+
Sbjct: 5   VAIE-EQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRV +GL  LGIK+GD IM LLPNSPEFVF FL AS+ G+I T ANPF+T  E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKAANVKLIVTMACFYDRVKDLA-ENGVKIVCVDFA-----VEGCLHFSVLSGADESHAP 180
           AKA+N KLI+T  C+ D+VKD A ENGVKI+ +D           LHFS L+GADE+  P
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMP 184

Query: 181 PVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLP 240
            V+ + D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY H DDVLLCVLP
Sbjct: 185 KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 244

Query: 241 FFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEF 300
            FHIYSLNS+LLCGLRAG+ ILIMQKFEIV  L+L++KY+V++ P VPPI LAIAKS   
Sbjct: 245 LFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVV 304

Query: 301 EKYDVSSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFP 360
           + YD+SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVLAM LAFAKEPF 
Sbjct: 305 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 364

Query: 361 VKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEG 420
           +K+GACGTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGYLND E+T RT+DKEG
Sbjct: 365 IKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEG 424

Query: 421 WLHTGDIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQ 480
           WLHTGDIG+ID+DDELFIVDRLKELIK+K FQVAPAELEALLLTHP +SDAAV+ M DE 
Sbjct: 425 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEA 484

Query: 481 AGEVPVAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRA 540
           AGEVPVAFVVK+NG  ITE+++KQFISKQV+FYKR+ RVFFV AIPKAPSGKILRK+LRA
Sbjct: 485 AGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRA 544

BLAST of Lcy05g003770 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.1e-289
Identity = 504/542 (92.99%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQ  +KFGSRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LT+RRVA+GLH LGIKKGDV+MNLLPNSPEFVFTFL ASYRGAIMTAANPFYT VEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAANVKLIVTMACFYDRVKDLAENGVKIVCVD+AVE CLH+SVLSGADESH P VDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKY+VS+MPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID DDELFIVDRLKELIKFKAFQVAPAELEALL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lcy05g003770 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 6.0e-289
Identity = 501/542 (92.44%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQ  +KFGSRPCLINGATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRVA GLH LGIKKGDV+MNLLPNSPEFVFTFL ASY GA+MTAANPFYT VEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADESH PPV+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK++VS+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELE+LL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lcy05g003770 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.3e-288
Identity = 499/542 (92.07%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQ  +KFGSRPCLINGATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRVA GLH LGIKK DV+MNLLPNSPEFVFTFLAASY GA+MTAANPFYT VEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADESH P V+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL+L+EK++VS+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELE+LL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lcy05g003770 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.5e-287
Identity = 498/542 (91.88%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQ  +K GSRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LT+RRVA+GLH LGIKKG+V+MNLLPNSPEFVFTFL ASYRGAIMTAANPFYT VEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAANVKLIVTMACFYDRVKDLAENGVKIVCVD+AVEGCLH+SVLSGADESH P VDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKY+VS+MPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGF+D+++ELFIVDRLKELIKFKAFQVAPAELEALL+THPKLSDAAVIGM DE+AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDV QFIS+QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Lcy05g003770 vs. ExPASy TrEMBL
Match: A0A5A7US61 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001380 PE=4 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.7e-281
Identity = 489/542 (90.22%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MA E+NQT EFIFRSKLPDIHIPNHLPLH+YVFQ   KF SRPCLINGATG VY+Y DVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LT+RRVA GLH LGIKKGDV+MNLLPNSPEFVFTFL ASYRGAIMTAANPFYT VEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADES AP VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG NPNLYYH DDV+L VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GA ILIMQKF+IVSLLQL+EK+++S+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELEALL+THPKLSDAAVIGM D +AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Lcy05g003770 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.1e-289
Identity = 505/542 (93.17%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQ  +KFGSRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LT+RRVA+GLH LGIKKGDV+MNLLPNSPEFVFTFL ASYRGAIMTAANPFYT VEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAANVKLIVTMACFYDRVKDLAENGVKIVCVD+AVE CLH+SVLSGADESH P VDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKY+VS+MPIVPPIFLAIAKSPE EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELEALL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lcy05g003770 vs. NCBI nr
Match: XP_022959958.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.3e-289
Identity = 504/542 (92.99%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQ  +KFGSRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           LT+RRVA+GLH LGIKKGDV+MNLLPNSPEFVFTFL ASYRGAIMTAANPFYT VEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAANVKLIVTMACFYDRVKDLAENGVKIVCVD+AVE CLH+SVLSGADESH P VDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS LQLVEKY+VS+MPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID DDELFIVDRLKELIKFKAFQVAPAELEALL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Lcy05g003770 vs. NCBI nr
Match: XP_023547628.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.2e-288
Identity = 502/542 (92.62%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQ  +KFGSRPCLINGATG V+SY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVHSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRVA GLH LGIKKGDV+MNLLPNSPEFVFTFLAASY GA+MTAANPFYT VEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADESH P V+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK++VS+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELE+LL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lcy05g003770 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.2e-288
Identity = 501/542 (92.44%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQ  +KFGSRPCLINGATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRVA GLH LGIKKGDV+MNLLPNSPEFVFTFL ASY GA+MTAANPFYT VEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADESH PPV+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+L+EK++VS+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELE+LL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lcy05g003770 vs. NCBI nr
Match: XP_022953208.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.7e-288
Identity = 499/542 (92.07%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQ  +KFGSRPCLINGATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQ 120
           L SRRVA GLH LGIKK DV+MNLLPNSPEFVFTFLAASY GA+MTAANPFYT VEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESHAPPVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADESH P V+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG NPNLYYHG DV+LCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL+L+EK++VS+MPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGAC 360
           SVR+LKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL MSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELE+LL+THPKLSDAAVIGM DEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGG ITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Lcy05g003770 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 758.4 bits (1957), Expect = 3.8e-219
Identity = 375/543 (69.06%), Postives = 448/543 (82.50%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQ  ++F ++PCLING TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQAKAAN 125
           +A   H LG+ + DV+M LLPN PEFV +FLAAS+RGA  TAANPF+TP EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 VKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCLHFSVLSGADESHAPPVD-- 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D  
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 -FAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFF 245
             + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY+H DDV+LCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ +V+V P+VPPI LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVK 365
           YD+SS+R++KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVLAMSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAG 485
           HTGDIG ID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 540
           EVPVAFVVK+    ++E+DVKQF+SKQVVFYKR+ +VFF  +IPKAPSGKILRK+LRAKL
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Lcy05g003770 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 752.3 bits (1941), Expect = 2.7e-217
Identity = 367/539 (68.09%), Postives = 451/539 (83.67%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQLTSRRVA 67
           +N+ IFRS+LPDI+IPNHLPLH+Y+F+  ++F ++PCLING TG VY+Y DV +TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  TGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQAKAANVK 127
            GLH LG+K+ DV+M LLPNSPE V TFLAAS+ GAI T+ANPF+TP EI+KQAKA+  K
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDFAV--EGCLHFSVLSGADESHAP--PVDFAADDV 187
           LIVT + + D++K+L  +GV IV  D     E CL FS L+ ++E      P   + +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY++ DDV+LCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVSSVR 307
           I+LC LR GA ILIM KFEI  LL+ +++ +V+V  +VPPI LAIAKSPE EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 ILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGACGTV 367
           ++KSG APLGKELEDA+ AKFP A LGQGYGMTEAGPVLAMSL FAKEPFPVK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  T+DK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPVAFV 487
           ID+DDELFIVDRLKELIK+K FQVAPAELE+LL+ HP+++D AV+ M +E AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 543
           V++    I+E+++KQF+SKQVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Lcy05g003770 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 704.1 bits (1816), Expect = 8.5e-203
Identity = 348/508 (68.50%), Postives = 416/508 (81.89%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQ  ++F ++PCLING TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQAKAAN 125
           +A   H LG+ + DV+M LLPN PEFV +FLAAS+RGA  TAANPF+TP EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 VKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCLHFSVLSGADESHAPPVD-- 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D  
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 -FAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFF 245
             + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY+H DDV+LCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ +V+V P+VPPI LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVK 365
           YD+SS+R++KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVLAMSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAG 485
           HTGDIG ID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKANGGTITEEDVKQFISKQV 505
           EVPVAFVVK+    ++E+DVKQF+SKQV
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Lcy05g003770 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 682.2 bits (1759), Expect = 3.5e-196
Identity = 332/546 (60.81%), Postives = 426/546 (78.02%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQTNAKFG----SRPCLINGATGHVYSYDDV 63
           E   +++FIFRSKLPDI IPNHLPL +YVFQ  +  G    S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTSRRVATGLHTLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAK 123
           Q   RR+A G+H LGI+ GDV+M LLPNSPEF  +FLA +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANVKLIVTMACFYDRVKDLAENGVKIVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+  K+I+T  C  D++ +L  +GV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHAPPVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLL 243
           +       + +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG NPNL +  +DV+L
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +FE+  +++L+++Y+V+V+P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRILKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAK 363
           SPE E+YD+SSVRI+ SG A L KELEDAVR KFP A+ GQGYGMTE+G V A SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTV 423
            PF  K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RT+
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDEDDELFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGM 483
           DK+GWLHTGDIGF+D+DDE+FIVDRLKELIKFK +QVAPAELEALL++HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 ADEQAGEVPVAFVVKANGGTITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            DE A EVPVAFV ++ G  +TE+DVK +++KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Lcy05g003770 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 670.2 bits (1728), Expect = 1.4e-192
Identity = 332/527 (63.00%), Postives = 412/527 (78.18%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNHLPLHEYVFQTNAKFGSRPCLINGATGHVYSYDDVQLTSRRVATGLH 71
           IFRSKLPDI IPNHLPLH Y F+  +    +PCLI G+TG  Y+Y +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  TLGIKKGDVIMNLLPNSPEFVFTFLAASYRGAIMTAANPFYTPVEIAKQAKAANVKLIVT 131
            LGI+KGDVIM LL NS EFVF+F+ AS  GA+ T ANPFYT  E+ KQ K++  KLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESH--APPVDFAADDVVALPYS 191
            + + D++K+L EN   I   +   E CL FS L   DE++     VD   DD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQIDGHNPNLYYHGDDVLLCVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY   +DV+LCVLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAILIMQKFEIVSLLQLVEKYRVSVMPIVPPIFLAIAKSPEFEKYDVSSVRILKSGG 311
           R+GA +L+M KFEI +LL L++++RV++  +VPP+ +A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLAMSLAFAKEPFPVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P A+LGQGYGMTEAGPVL+MSL FAKEP P K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGASLPANSAGEICIRGDQIMKGYLNDSESTKRTVDKEGWLHTGDIGFIDEDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  T+D+EGWLHTGDIG++DEDDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLLTHPKLSDAAVIGMADEQAGEVPVAFVVKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LL+ H  ++DAAV+   DE AGEVPVAFVV++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 TITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 537
            ITEEDVK++++KQVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560