Lcy05g003680 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g003680
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAspartyl protease family protein
LocationChr05: 3284670 .. 3288359 (-)
RNA-Seq ExpressionLcy05g003680
SyntenyLcy05g003680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGAAAGCCAGGCCTTCATCTTTTGTTTCCATGCTAAACTCCAATTCCTAAGAATCTCTTTCCCTCCATTTCTTTTCCCTTTCTTTTCCCAATCTTCTTCTCAGCTCTTTCTCTCAGACATGGAGATTGCAAAATCTTTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCCGTCGACACTCGCTCGGTCGGCCCCCATAATCACCATCATAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCATCTTTCAGAAGCCAAGTGAGTGTTCCAAAAAAAATCCCATTCAAAAAATGGGGGGAAAAAATCTCTCTTAATATCAATGTTGATTATTGTTATATTAATTAATTTTCGGATCTGTTTATTTTTTTTTGGTTGAATGTGGCTTTTTTGCTTTGCTTTTGCTAGGATCTAGGATTTGGCTTTTTTTTTTTTTTTTCCTTTCTATTTTTGCTTTAAAAGATGAACCCTCATCATTGGGTTTGATTTAATTTCTAGTTAAACTAGTCTTTTTTGGGGCTTTTGGATGATTATGTGAATCTCTTTTAAAAGGGTCTTTTTTTATTAATACATCACTCTATTTTCTTAAATTACATTTGGGTTTTTTTGAAGAACAATTTGTTTTGAGCAAAAAAAAAAACAAAATGTCATTTTTTTGTGGACTTATCTCACTATACCTTCTCTCTCTTCTGCTGTTTAGAACTTCAATGTTTTTTTTTTTTTGAAATATTTTTTCCTTTTTCCTTTTGGGAAACTATATGCATCAATTAATAAATAATGAACTTTTTTTTTTCCTTCTAATTGTTCATTAGTAATTATAAAGAAATGTGATGTGGGGAGAATAGGGATGGATTAAGAAATATTTCAGCCTGGTAATATATTTGAGTTAGGACCGATTAAGCTTCTTTATTTTAGTTGGTGATTTGAAAAAGAAAAATTAAAGAGCTCTGGACTGAAATGAATACCAAGGATAAAAGTTTTTTTTTTTTTTTTGACAACGTTTATAATTCAATCTAATCCTACATGGATTGGAGTTCGGTTAGATTTGTTTTGAATTTATTGAATTTATAGGTCACTAAATTTTATGAATTTCTTTATAAAAAAAAAATTATGAATTTCCTAACTGTGGTCCTCGTTTTTTTTTTTTTTTTTTTTTTTGTTTAAATTCTATAGAGTAGAGTTCACTCTTTTGACTGACTGGTCTTTTATCATTTTAGTTCTTAGATAGACCTTAGGATTTTACTTTATTAGAATTATAAGGTCGATAGAATATAAATATGAGAAGATAAAAATAGAAATAAATAAAATATGATAAAAGTCTTTTCACTGGCATACTGCATATTATAAAATTTTAACAATTTATATCTAATAAAAATTCCAAAATTACCCTTGATTGGTAAGTTGGGTGGGTAGGTAACATATTTTCATGCTTTACATTAAAGGTTATTTTGGTAATTAAAAAATTTAGAGGGATGCAAGCAAAAGCTTCTTTGTTTTTCCATCCAACCGTCTACTAACTCTATCTATCTACTAATTTGATTTATTTTTATTTGTTCAACAACCTGTGGTTAGTTATTTTTATTAAAATTGATACGGTAAGATTTAACATAAAAGTATGATATTTGAGGTTTTTTTTTTAGTAGTAACTTTATGTCAAATATGCTTATGTTGACAAAACTAAAGTCTAAATGGACGATGCTGCCTTGCTTGAGGTCTTGAGCTTAGAATTTTTATAGTTTATATAAATACTTTAGTAATTTTTGAATCTAATGTCTTCCTAGTCAATAAGTTATGTTTGGACCACAATTTTTTTCATAATGTTTATCTCTATAAATACGCGAAGGTTCCAGGTTTGAAACCTAGATAGACCACTTTTAAAAAACAAAATTGTTAAATGTGGTACCATATATTAGATTTGATGCGAGGTTCAACTTAGAGTAGACATACTTTTCACAAATAGGTCGTGTCATGTTAATTTATTCAATGTATTTATTAACAATTCAGATATTTTAGATCAGTTGAACTTTATTTAAGTTGATGTTAACACCATTTTTGTGGTTTTTATTTTTTTTGAATTGCAGAGGTGGGAAAGGGAGCAACCACATTGCAAATGAAAGAAAGAGACGAGTGTTCAGGCAAAGTGAGGGACTGGGACGAGAATCTCCAAAACCGCGTACTCCTCGACGCCAGTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCCCAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCCTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCGGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCGTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCACCTGTGCGCCTGGGACTGGGAATTCAAACCCCTGTAATTATGAGATTTCCTATGGCGATGGATCTTATTCCAAAGGGGACCTTGCATTCGAGAGGCTGACTTTGGGGAAGATTACCGTCGACAACTTTGTAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGGAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATTGTGTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGCTCTTTAACGATGGGGGGTGGGGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAAATCCTCAGATGCCAAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAACTGAACGTGCCGCGTTTGGCTCCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTGTGATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAAAGTCCTGGATTGCAAAAAGTGGCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTACGCAAATGAAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGAACGTTGATGTTCAAGGGATACTTTACTTTTTGAAATCTGATTCTTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGATGAGGATCAGTTGGGGATTATCGGGAATTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAATGGAGACTTGCAGTTTCTAGTTGGGATCAGTGATTTTGTTTTTCCGGGAAAGTGGAAGGAATTTTCCCAGAAAATTCTTTTATACATAGATTGGATATATTTCAGAAGCCGGGCGGCCGACCTTGATTATTACTGTAAGTGGATGGGGGAGTGGAATTCTTGAGTTGAATTATCTCATTTCTTCTCGAGAAGTTAAAAAATGGAGAGAAATTTGGAATATGTATATTTCATTTTTTGCTTCTCTTGTAATCTGTAAATTGGAATTCAAATTATTGCTATTAATTGATACGTCTCTCTTCTTCTT

mRNA sequence

ACGAAAGCCAGGCCTTCATCTTTTGTTTCCATGCTAAACTCCAATTCCTAAGAATCTCTTTCCCTCCATTTCTTTTCCCTTTCTTTTCCCAATCTTCTTCTCAGCTCTTTCTCTCAGACATGGAGATTGCAAAATCTTTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCCGTCGACACTCGCTCGGTCGGCCCCCATAATCACCATCATAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCATCTTTCAGAAGCCAAAGGTGGGAAAGGGAGCAACCACATTGCAAATGAAAGAAAGAGACGAGTGTTCAGGCAAAGTGAGGGACTGGGACGAGAATCTCCAAAACCGCGTACTCCTCGACGCCAGTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCCCAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCCTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCGGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCGTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCACCTGTGCGCCTGGGACTGGGAATTCAAACCCCTGTAATTATGAGATTTCCTATGGCGATGGATCTTATTCCAAAGGGGACCTTGCATTCGAGAGGCTGACTTTGGGGAAGATTACCGTCGACAACTTTGTAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGGAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATTGTGTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGCTCTTTAACGATGGGGGGTGGGGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAAATCCTCAGATGCCAAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAACTGAACGTGCCGCGTTTGGCTCCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTGTGATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAAAGTCCTGGATTGCAAAAAGTGGCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTACGCAAATGAAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGAACGTTGATGTTCAAGGGATACTTTACTTTTTGAAATCTGATTCTTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGATGAGGATCAGTTGGGGATTATCGGGAATTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAATGGAGACTTGCAGTTTCTAGTTGGGATCAGTGATTTTGTTTTTCCGGGAAAGTGGAAGGAATTTTCCCAGAAAATTCTTTTATACATAGATTGGATATATTTCAGAAGCCGGGCGGCCGACCTTGATTATTACTGTAAGTGGATGGGGGAGTGGAATTCTTGAGTTGAATTATCTCATTTCTTCTCGAGAAGTTAAAAAATGGAGAGAAATTTGGAATATGTATATTTCATTTTTTGCTTCTCTTGTAATCTGTAAATTGGAATTCAAATTATTGCTATTAATTGATACGTCTCTCTTCTTCTT

Coding sequence (CDS)

ATGGAGATTGCAAAATCTTTTCATTCTCCTATTCTCCTCCTCCTTCTTCTCCTTCTCCTCTTCGCCGTCGACACTCGCTCGGTCGGCCCCCATAATCACCATCATAATAAGAAGCTTCTTCTTAACCTTCGGAACCTTCCATGGAGCCAGAATAAGAAAAGAGCACCAACTGATTGCATCTTTCAGAAGCCAAAGGTGGGAAAGGGAGCAACCACATTGCAAATGAAAGAAAGAGACGAGTGTTCAGGCAAAGTGAGGGACTGGGACGAGAATCTCCAAAACCGCGTACTCCTCGACGCCAGTCACGTCAAAGCCCTCCAATCCCGAGTCAGATCCGCCATTTCTCCCAACAAACCCCTGGGAATCTCCGAGTCCCAAATCCCCATATCCCTCGGCCAGCCCCTCCAAACCCTCAACTACGTCGTCACCGTCGGAATCGGCGGCCGGAATTTAACCCTCATCGTCGACACCGGCAGCGACATCACCTGGGTCCAGTGCCGCCCTTGCCGCAAATGTTACACCCAACAAGAACCCATTTTCGATCCCTCAAATTCCACTTCCTTCCTCTCCGTTCCTTGCAATTCCACCACCTGCTCCTCCCTCGTCTACGGCACCTGTGCGCCTGGGACTGGGAATTCAAACCCCTGTAATTATGAGATTTCCTATGGCGATGGATCTTATTCCAAAGGGGACCTTGCATTCGAGAGGCTGACTTTGGGGAAGATTACCGTCGACAACTTTGTAATTGGATGTGGTCAAACTAATGAGGGCTTGTACGGCGGAACTTCTGGGTTGATGGGTTTCGACAGAGATAAATTATCTATTGTGTCTCAAACATCCTCTGTTTTTGGTGGGATTTTTTCTTACTGTTTGCCTGCAACAGGGGATGGAACTTCAGGCTCTTTAACGATGGGGGGTGGGGATTTTTCCAGTTTCAGAAACGTCTCTCCAATTTCCTACACTAGAATGATCTCAAATCCTCAGATGCCAAGTTTTTACCTTTTGAACGTGACCGGAATTAGCATCGGCGGGGTGAAACTGAACGTGCCGCGTTTGGCTCCGGGTAATGGGGTTACGAGTTTACTCGATTCTGGAACTGTGATTACCAGGCTGCCTCCTTCGGTTTACGATGCTTTCAAAGCGGAATTTGAGAAGCAAAGTCCTGGATTGCAAAAAGTGGCAGGATATTCGATTTTGGAGGCTTGTTATAATCTTACAGAGGTTACGCAAATGAAACTTCCGTCTGTGAAATTTTACTTTGAAGGTGATGCAGTGCTGAACGTTGATGTTCAAGGGATACTTTACTTTTTGAAATCTGATTCTTCTCAGACCTGTTTGGCGTTTGCGAGATTGTCTGATGAGGATCAGTTGGGGATTATCGGGAATTACCAGCAGAAGAATCAGAGGGTCCTTTATAATACCCAGGAATCGAAGGTGGGTTTTGCAATGGAGACTTGCAGTTTCTAG

Protein sequence

MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCIFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQESKVGFAMETCSF
Homology
BLAST of Lcy05g003680 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 8.3e-77
Identity = 166/397 (41.81%), Postives = 231/397 (58.19%), Query Frame = 0

Query: 98  LDASHVKALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVTVGIG--GRNLTLIV 157
           LD + V ++ S++   ++ +       + +P   G  L + NY+VTVG+G    +L+LI 
Sbjct: 90  LDQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIF 149

Query: 158 DTGSDITWVQCRPC-RKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSN 217
           DTGSD+TW QC+PC R CY Q+EPIF+PS STS+ +V C+S  C SL   T   G+ +++
Sbjct: 150 DTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSAS 209

Query: 218 PCNYEISYGDGSYSKGDLAFERLTLGKITV-DNFVIGCGQTNEGLYGGTSGLMGFDRDKL 277
            C Y I YGD S+S G LA E+ TL    V D    GCG+ N+GL+ G +GL+G  RDKL
Sbjct: 210 NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKL 269

Query: 278 SIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFY 337
           S  SQT++ +  IFSYCLP++   T G LT G    S     +PIS     +     SFY
Sbjct: 270 SFPSQTATAYNKIFSYCLPSSASYT-GHLTFGSAGISRSVKFTPIS-----TITDGTSFY 329

Query: 338 LLNVTGISIGGVKLNVPRL---APGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGL 397
            LN+  I++GG KL +P      PG    +L+DSGTVITRLPP  Y A ++ F+ +    
Sbjct: 330 GLNIVAITVGGQKLPIPSTVFSTPG----ALIDSGTVITRLPPKAYAALRSSFKAKMSKY 389

Query: 398 QKVAGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFAR 457
              +G SIL+ C++L+    + +P V F F G AV+ +  +GI Y  K   SQ CLAFA 
Sbjct: 390 PTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFK--ISQVCLAFAG 449

Query: 458 LSDEDQLGIIGNYQQKNQRVLYNTQESKVGFAMETCS 488
            SD+    I GN QQ+   V+Y+    +VGFA   CS
Sbjct: 450 NSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of Lcy05g003680 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.3e-69
Identity = 149/427 (34.89%), Postives = 227/427 (53.16%), Query Frame = 0

Query: 72  TLQMKERDEC-SGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKP--LGISESQIP 131
           TL++  RD   S   R+    L  R+  D   V A+  R+   + P+      +++    
Sbjct: 60  TLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSD 119

Query: 132 ISLGQPLQTLNYVVTVGIGG--RNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNST 191
           I  G    +  Y V +G+G   R+  +++D+GSD+ WVQC+PC+ CY Q +P+FDP+ S 
Sbjct: 120 IVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSG 179

Query: 192 SFLSVPCNSTTCSSLVYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFERLTLGKITVDN 251
           S+  V C S+ C  +    C     +S  C YE+ YGDGSY+KG LA E LT  K  V N
Sbjct: 180 SYTGVSCGSSVCDRIENSGC-----HSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRN 239

Query: 252 FVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGG 311
             +GCG  N G++ G +GL+G     +S V Q S   GG F YCL + G  ++GSL  G 
Sbjct: 240 VAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG- 299

Query: 312 GDFSSFRNVSPI--SYTRMISNPQMPSFYLLNVTGISIGGVKLNVP-----RLAPGNGVT 371
                 R   P+  S+  ++ NP+ PSFY + + G+ +GGV++ +P         G+G  
Sbjct: 300 ------REALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 359

Query: 372 SLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVKFY 431
            ++D+GT +TRLP + Y AF+  F+ Q+  L + +G SI + CY+L+    +++P+V FY
Sbjct: 360 -VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 419

Query: 432 FEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQESKV 487
           F    VL +  +  L  +  DS   C AFA  +    L IIGN QQ+  +V ++     V
Sbjct: 420 FTEGPVLTLPARNFLMPV-DDSGTYCFAFA--ASPTGLSIIGNIQQEGIQVSFDGANGFV 470

BLAST of Lcy05g003680 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.4e-62
Identity = 144/393 (36.64%), Postives = 221/393 (56.23%), Query Frame = 0

Query: 99  DASHVKALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVTVGIG--GRNLTLIVD 158
           D + V+++ S++ S  S N+      +++P   G  L + NY+VT+GIG    +L+L+ D
Sbjct: 92  DQARVESIYSKL-SKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFD 151

Query: 159 TGSDITWVQCRPC-RKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSNP 218
           TGSD+TW QC PC   CY+Q+EP F+PS+S+++ +V C+S  C        A     SN 
Sbjct: 152 TGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED------AESCSASN- 211

Query: 219 CNYEISYGDGSYSKGDLAFERLTLGKITV-DNFVIGCGQTNEGLYGGTSGLMGFDRDKLS 278
           C Y I YGD S+++G LA E+ TL    V ++   GCG+ N+GL+ G +GL+G    KLS
Sbjct: 212 CVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLS 271

Query: 279 IVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYL 338
           + +QT++ +  IFSYCLP+    ++G LT G    S     +PIS      N      Y 
Sbjct: 272 LPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN------YG 331

Query: 339 LNVTGISIGGVKLNV-PRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKV 398
           +++ GIS+G  +L + P      G  +++DSGTV TRLP  VY   ++ F+++    +  
Sbjct: 332 IDIIGISVGDKELAITPNSFSTEG--AIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKST 391

Query: 399 AGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSD 458
           +GY + + CY+ T +  +  P++ F F G  V+ +D  GI   +K   SQ CLAFA   +
Sbjct: 392 SGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIK--ISQVCLAFA--GN 451

Query: 459 EDQLGIIGNYQQKNQRVLYNTQESKVGFAMETC 487
           +D   I GN QQ    V+Y+    +VGFA   C
Sbjct: 452 DDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of Lcy05g003680 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.0e-58
Identity = 142/416 (34.13%), Postives = 218/416 (52.40%), Query Frame = 0

Query: 89  DENLQNRVLLDASHVK-----ALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVT 148
           DE   +R+  D+  VK     A Q   R+     +P G S S +    G    +  Y   
Sbjct: 89  DELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVS---GLSQGSGEYFTR 148

Query: 149 VGIG--GRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSL 208
           +G+G   R + +++DTGSDI W+QC PCR+CY+Q +PIFDP  S ++ ++PC+S  C  L
Sbjct: 149 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 208

Query: 209 VYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFERLTLGKITVDNFVIGCGQTNEGLYGG 268
               C         C Y++SYGDGS++ GD + E LT  +  V    +GCG  NEGL+ G
Sbjct: 209 DSAGC---NTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVG 268

Query: 269 TSGLMGFDRDKLSIVSQTSSVFGGIFSYCL-PATGDGTSGSLTMGGGDFSSFRNVSPISY 328
            +GL+G  + KLS   QT   F   FSYCL   +      S+  G    S     +P   
Sbjct: 269 AAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTP--- 328

Query: 329 TRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAP--------GNGVTSLLDSGTVITRLP 388
             ++SNP++ +FY + + GIS+GG +  VP +          GNG   ++DSGT +TRL 
Sbjct: 329 --LLSNPKLDTFYYVGLLGISVGGTR--VPGVTASLFKLDQIGNGGV-IIDSGTSVTRLI 388

Query: 389 PSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQG 448
              Y A +  F   +  L++   +S+ + C++L+ + ++K+P+V  +F G    +V +  
Sbjct: 389 RPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG---ADVSLPA 448

Query: 449 ILYFLKSDSS-QTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQESKVGFAMETCS 488
             Y +  D++ + C AFA       L IIGN QQ+  RV+Y+   S+VGFA   C+
Sbjct: 449 TNYLIPVDTNGKFCFAFA--GTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of Lcy05g003680 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.6e-58
Identity = 138/437 (31.58%), Postives = 237/437 (54.23%), Query Frame = 0

Query: 72  TLQMKERDE-CSGKVRDWDENLQNRVLLDASHVKALQSRVRSAI-----SPNKPLGISES 131
           +L++  RD   + + +D+     +R+  D+S V  + +++R A+     S  KP+   ++
Sbjct: 81  SLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDT 140

Query: 132 Q-------IPISLGQPLQTLNYVVTVGIG--GRNLTLIVDTGSDITWVQCRPCRKCYTQQ 191
           +        P+  G    +  Y   +G+G   + + L++DTGSD+ W+QC PC  CY Q 
Sbjct: 141 RYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQS 200

Query: 192 EPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFER 251
           +P+F+P++S+++ S+ C++  CS L    C      SN C Y++SYGDGS++ G+LA + 
Sbjct: 201 DPVFNPTSSSTYKSLTCSAPQCSLLETSAC-----RSNKCLYQVSYGDGSFTVGELATDT 260

Query: 252 LTLGKI-TVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 311
           +T G    ++N  +GCG  NEGL+ G +GL+G     LSI +Q  +     FSYCL    
Sbjct: 261 VTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD 320

Query: 312 DGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRL--- 371
            G S SL     DF+S +     +   ++ N ++ +FY + ++G S+GG K+ +P     
Sbjct: 321 SGKSSSL-----DFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFD 380

Query: 372 --APGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQK-VAGYSILEACYNLTEVT 431
             A G+G   +LD GT +TRL    Y++ +  F K +  L+K  +  S+ + CY+ + ++
Sbjct: 381 VDASGSGGV-ILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 440

Query: 432 QMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQR 487
            +K+P+V F+F G   L++  +  L  +  DS   C AFA  S    L IIGN QQ+  R
Sbjct: 441 TVKVPTVAFHFTGGKSLDLPAKNYLIPV-DDSGTFCFAFAPTS--SSLSIIGNVQQQGTR 500

BLAST of Lcy05g003680 vs. ExPASy TrEMBL
Match: A0A6J1JS16 (aspartyl protease family protein At5g10770 OS=Cucurbita maxima OX=3661 GN=LOC111488391 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 6.8e-175
Identity = 315/493 (63.89%), Postives = 379/493 (76.88%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCI 60
           MEI+KS     LLLLLLLL F  + RS   +        LL+L+ LPW Q ++ A  +CI
Sbjct: 1   MEISKSL-CFFLLLLLLLLFFVDEARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVVNCI 60

Query: 61  FQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPL 120
           FQKP+V +G TTL+MKERD CSGKV DW  NLQNR++ DA  +++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQNNLQNRLIFDAIRLQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSVPCNSTTCSSLV-----YGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFE 240
           DPSNS+SFLS+ CNS TC +L       G C  G GNS+ C YEI+YGDGSYS+G+L FE
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPG 360
            G SGSLTMGGGDFS++RNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V    P 
Sbjct: 301 AGASGSLTMGGGDFSNYRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----PN 360

Query: 361 NGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPS 420
           NG  SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P+
Sbjct: 361 NGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIPT 420

Query: 421 VKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQ 480
           VKF+FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN++
Sbjct: 421 VKFFFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNSK 480

Query: 481 ESKVGFAMETCSF 489
           ES VGFA E C F
Sbjct: 481 ESTVGFAAEPCGF 485

BLAST of Lcy05g003680 vs. ExPASy TrEMBL
Match: A0A0A0K8J2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G431320 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 4.4e-174
Identity = 312/499 (62.53%), Postives = 383/499 (76.75%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLL---LLLFAVDTRSVG---PHNHHHNKKLLLNLRNLPWSQNKKR 60
           MEI+KS H P+ LLLLL   LL   VD RS      +  +H K LL   +N PW ++ + 
Sbjct: 1   MEISKSLHFPLSLLLLLLLPLLSIGVDARSSSFNLGNGDNHEKGLLQLFQNFPWKEHGE- 60

Query: 61  APTDCIFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAI 120
           A  +CIFQKPK+ KG TTL+MK+RD CSGK+ DW++  QNR++LDA +V +L S  +SAI
Sbjct: 61  AVVNCIFQKPKITKGITTLEMKQRDYCSGKITDWEKIFQNRIILDAINVNSLFSHFKSAI 120

Query: 121 SPNKPLGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYT 180
            P +   +S+SQIPIS G  LQTLNY+VTVGIGG+N TLIVDTGSD+TWVQC PCR CY 
Sbjct: 121 FPGQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYN 180

Query: 181 QQEPIFDPSNSTSFLSVPCNSTTCSSL-----VYGTCAPGTGNSNPCNYEISYGDGSYSK 240
           QQEP+F+PSNS+SFLS+PCNS TC +L       G C+    NS  C+Y+I YGDGSYS+
Sbjct: 181 QQEPLFNPSNSSSFLSLPCNSPTCVALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSR 240

Query: 241 GDLAFERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSY 300
           G+L FE+LTLGK  +DNF+ GCG+ N+GL+GG SGLMG  R +LS+VSQTSS+FG +FSY
Sbjct: 241 GELGFEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSLFGSVFSY 300

Query: 301 CLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNV 360
           CLP TG G+SGSLT+GG DFS+F+N+SPISYTRMI NPQM +FY LN+TGISIGGV LNV
Sbjct: 301 CLPTTGVGSSGSLTLGGADFSNFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNV 360

Query: 361 PRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVT 420
           PRL+   GV SLLDSGTVITRL PS+Y AFKAEFEKQ  G +   G+SIL  C+NLT   
Sbjct: 361 PRLSSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYE 420

Query: 421 QMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQR 480
           ++ +P+VKF FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQR
Sbjct: 421 EVNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQR 480

Query: 481 VLYNTQESKVGFAMETCSF 489
           V+YN++ESKVGFA E CSF
Sbjct: 481 VIYNSKESKVGFAGEPCSF 496

BLAST of Lcy05g003680 vs. ExPASy TrEMBL
Match: A0A1S3CDQ0 (aspartyl protease family protein At5g10770 OS=Cucumis melo OX=3656 GN=LOC103499859 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 5.8e-174
Identity = 313/497 (62.98%), Postives = 384/497 (77.26%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLL---LLLFAVDTR-SVGPHNHHHNKKLLLNLRNLPWSQNKKRAP 60
           ME++KS H P+ LL LL   LL   VD R SVG   ++H K LL   +N PW ++ + A 
Sbjct: 3   MEVSKSLHFPLSLLFLLLLPLLFIIVDARSSVGNGGNYHEKGLLQLFQNFPWKEHGE-AV 62

Query: 61  TDCIFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISP 120
            +CIFQKPK+ KG TTL+MK+RD CSGK+ D ++  QNR++LDA +V +L S V+SAI P
Sbjct: 63  VNCIFQKPKITKGITTLEMKQRDYCSGKITDLEKIFQNRIILDAINVNSLLSHVKSAIFP 122

Query: 121 NKPLGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQ 180
            +   +S+SQIPIS G  LQTLNY+VTVGIGG+N TLIVDTGSD+TWVQC PCR CY QQ
Sbjct: 123 GQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYNQQ 182

Query: 181 EPIFDPSNSTSFLSVPCNSTTCSSL-----VYGTCAPGTGNSNPCNYEISYGDGSYSKGD 240
           EP+F+PSNS+SFLS+PC+S TC +L       G C+    NS  C+Y+I YGDGSYS+G+
Sbjct: 183 EPLFNPSNSSSFLSLPCSSPTCLALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSRGE 242

Query: 241 LAFERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCL 300
           L +E+LTLGK  +DNF+ GCG+ N+GL+GG SGLMG  R +LS+VSQTSSVFG IFSYCL
Sbjct: 243 LGYEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSVFGSIFSYCL 302

Query: 301 PATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPR 360
           P TG G+SGSLT+GG DFSSF+N+SPISYTRMI NPQM +FY LN+TGISIGGV LNVPR
Sbjct: 303 PTTGVGSSGSLTLGGTDFSSFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNVPR 362

Query: 361 LAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQM 420
           L+   GV SLLDSGTVITRL PS+Y AFKAEFEKQ  G +   G+SIL  C+NLT   ++
Sbjct: 363 LSSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYEEV 422

Query: 421 KLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVL 480
            +P+VKF FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+
Sbjct: 423 NIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQRVV 482

Query: 481 YNTQESKVGFAMETCSF 489
           YN++ESKVGFA E CSF
Sbjct: 483 YNSKESKVGFAGEPCSF 496

BLAST of Lcy05g003680 vs. ExPASy TrEMBL
Match: A0A6J1GQV9 (aspartyl protease family protein At5g10770 OS=Cucurbita moschata OX=3662 GN=LOC111456281 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.8e-173
Identity = 313/493 (63.49%), Postives = 376/493 (76.27%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCI 60
           MEI+KS       LLLLLL F    RS   +        LL+L+  PW Q ++ A  +CI
Sbjct: 1   MEISKSL---CFFLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKRPWKQ-QEEAVINCI 60

Query: 61  FQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPL 120
           FQKP+V +G TTL+MKE+D CSG+V DW +NLQNR++ DA HV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKEKDYCSGEVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSVPCNSTTCSSLV-----YGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFE 240
           DPSNS+SFLS+ CNS TC +L       G C  G GNS+ C YEI+YGDGSYS+G+L FE
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPG 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V      
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----SN 360

Query: 361 NGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPS 420
           NG  SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P+
Sbjct: 361 NGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIPT 420

Query: 421 VKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQ 480
           VKFYFEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN++
Sbjct: 421 VKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNSK 480

Query: 481 ESKVGFAMETCSF 489
           ES VGFA E C F
Sbjct: 481 ESTVGFAAEPCGF 483

BLAST of Lcy05g003680 vs. ExPASy TrEMBL
Match: A0A5A7UYY6 (Aspartyl protease family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001610 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.9e-172
Identity = 313/499 (62.73%), Postives = 385/499 (77.15%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFA--VDTRS----VGPHNHHHNKKLLLNLRNLPWSQNKKR 60
           ME++KS H P+ LL LLL L +  VD RS    VG +  +H K LL   +N PW ++ + 
Sbjct: 3   MEVSKSLHFPLSLLFLLLPLLSIIVDARSSSFGVG-NGSNHEKGLLQLFQNFPWKEHGE- 62

Query: 61  APTDCIFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAI 120
           A  +CIFQKPK+ KG TTL+MK+RD CSGK+ D ++  QNR++LDA +V +L S V+SAI
Sbjct: 63  AVVNCIFQKPKITKGITTLEMKQRDYCSGKITDLEKIFQNRIILDAINVNSLLSHVKSAI 122

Query: 121 SPNKPLGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYT 180
            P +   +S+SQIPIS G  LQTLNY+VTVGIGG+N TLIVDTGSD+TWVQC PCR CY 
Sbjct: 123 FPGQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYN 182

Query: 181 QQEPIFDPSNSTSFLSVPCNSTTCSSL-----VYGTCAPGTGNSNPCNYEISYGDGSYSK 240
           QQEP+F+PSNS+SFLS+PC+S TC +L       G C+    NS  C+Y+I YGDGSYS+
Sbjct: 183 QQEPLFNPSNSSSFLSLPCSSPTCLALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSR 242

Query: 241 GDLAFERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSY 300
           G+L +E+LTLGK  +DNF+ GCG+ N+GL+GG SGLMG  R +LS+VSQTSSVFG IFSY
Sbjct: 243 GELGYEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSVFGSIFSY 302

Query: 301 CLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNV 360
           CLP TG G+SGSLT+GG DFSSF+N+SPISYTRMI NPQM +FY LN+TGISIGGV LNV
Sbjct: 303 CLPTTGVGSSGSLTLGGTDFSSFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNV 362

Query: 361 PRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVT 420
           PRL+   GV SLLDSGTVITRL PS+Y AFKAEFEKQ  G +   G+SIL  C+NLT   
Sbjct: 363 PRLSSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYE 422

Query: 421 QMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQR 480
           ++ +P+VKF FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQR
Sbjct: 423 EVNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQR 482

Query: 481 VLYNTQESKVGFAMETCSF 489
           V+YN++ESKVGFA E CSF
Sbjct: 483 VVYNSKESKVGFAGEPCSF 497

BLAST of Lcy05g003680 vs. NCBI nr
Match: KAG6575642.1 (Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630.2 bits (1624), Expect = 1.5e-176
Identity = 321/494 (64.98%), Postives = 383/494 (77.53%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVD-TRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDC 60
           MEI+KS    +LLLLLLLLLF VD  RS   +        LL+L+ LPW Q ++ A  +C
Sbjct: 1   MEISKSLCFFLLLLLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVINC 60

Query: 61  IFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKP 120
           IFQKP+V +G TTL+MKERD CSGKV DW +NLQNR++ DA HV++LQSR++SAI     
Sbjct: 61  IFQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDT 120

Query: 121 LGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPI 180
             IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+
Sbjct: 121 HQISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPL 180

Query: 181 FDPSNSTSFLSVPCNSTTCSSLV-----YGTCAPGTGNSNPCNYEISYGDGSYSKGDLAF 240
           FDPSNS+SFLS+ CNS TC +L       G C  G GNS+ C YEI+YGDGSYS+G+L F
Sbjct: 181 FDPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GYGNSSSCGYEINYGDGSYSRGELGF 240

Query: 241 ERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPAT 300
           ERL LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+T
Sbjct: 241 ERLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPST 300

Query: 301 GDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAP 360
           G G SGSLTMGGGDFS+FRNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V     
Sbjct: 301 GAGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----S 360

Query: 361 GNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLP 420
            NG  SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P
Sbjct: 361 NNGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIP 420

Query: 421 SVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNT 480
           +VKFYFEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN+
Sbjct: 421 TVKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNS 480

Query: 481 QESKVGFAMETCSF 489
           +ES VGFA E C F
Sbjct: 481 KESTVGFAAEPCGF 487

BLAST of Lcy05g003680 vs. NCBI nr
Match: KAG7014194.1 (Aspartyl protease family protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.0 bits (1621), Expect = 3.4e-176
Identity = 318/493 (64.50%), Postives = 380/493 (77.08%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCI 60
           MEI+KS    +LLLLLLLL F    RS   +        LL+L+ LPW Q ++ A  +CI
Sbjct: 1   MEISKSLCFFLLLLLLLLLFFVDQARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVINCI 60

Query: 61  FQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPL 120
           FQKP+V +G TTL+MKERD CSGKV DW +NLQNR++ DA HV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSVPCNSTTCSSLV-----YGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFE 240
           DPSNS+SFLS+ CNS TC +L       G C  G GNS+ C YEI+YGDGSYS+G+L FE
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GYGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPG 360
            G SGSLTMGGGDFS+FRNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V      
Sbjct: 301 AGASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----SN 360

Query: 361 NGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPS 420
           NG  SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P+
Sbjct: 361 NGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIPT 420

Query: 421 VKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQ 480
           VKFYFEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN++
Sbjct: 421 VKFYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNSK 480

Query: 481 ESKVGFAMETCSF 489
           ES VGFA E C F
Sbjct: 481 ESTVGFAAEPCGF 486

BLAST of Lcy05g003680 vs. NCBI nr
Match: XP_023547381.1 (aspartyl protease family protein At5g10770 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.1 bits (1616), Expect = 1.3e-175
Identity = 316/491 (64.36%), Postives = 378/491 (76.99%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCI 60
           MEI+KS      LLLLLLL F    RS   +        LL+L+ LPW Q ++ A  +CI
Sbjct: 1   MEISKSL--CFFLLLLLLLFFVDQARSAAINGDSEKLHRLLHLQKLPWKQ-QEEAVINCI 60

Query: 61  FQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPL 120
           FQKP+V +G TTL+MKERD CSGKV DW +NLQNR++ DA HV++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQKNLQNRLISDAIHVQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSVPCNSTTCSSLVYGTCAPGT---GNSNPCNYEISYGDGSYSKGDLAFERL 240
           DPSNS+SFLS+ CNS TC +L   T   G    GNS+ C YEI+YGDGSYS+G+L FERL
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPSATGNSGLCGYGNSSSCGYEINYGDGSYSRGELGFERL 240

Query: 241 TLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDG 300
            LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG G
Sbjct: 241 NLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTGAG 300

Query: 301 TSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPGNG 360
            SGSLTMGGGDFS+FRNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V    P NG
Sbjct: 301 ASGSLTMGGGDFSNFRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----PNNG 360

Query: 361 VTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVK 420
             SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P+VK
Sbjct: 361 ALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIPTVK 420

Query: 421 FYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQES 480
           FYFEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN++ES
Sbjct: 421 FYFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNSKES 480

Query: 481 KVGFAMETCSF 489
            +GFA E C F
Sbjct: 481 TLGFAAEPCGF 484

BLAST of Lcy05g003680 vs. NCBI nr
Match: XP_022991886.1 (aspartyl protease family protein At5g10770 [Cucurbita maxima])

HSP 1 Score: 623.6 bits (1607), Expect = 1.4e-174
Identity = 315/493 (63.89%), Postives = 379/493 (76.88%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKKRAPTDCI 60
           MEI+KS     LLLLLLLL F  + RS   +        LL+L+ LPW Q ++ A  +CI
Sbjct: 1   MEISKSL-CFFLLLLLLLLFFVDEARSDAINGDSEKLHRLLHLQKLPWKQ-QEEAVVNCI 60

Query: 61  FQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPL 120
           FQKP+V +G TTL+MKERD CSGKV DW  NLQNR++ DA  +++LQSR++SAI      
Sbjct: 61  FQKPRVREGITTLEMKERDYCSGKVTDWQNNLQNRLIFDAIRLQSLQSRIKSAIFSGDTH 120

Query: 121 GISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIF 180
            IS+SQIP+S G  LQTLNY+VTV +GGR+ TLIVDTGSD+TWVQCRPCR CY QQEP+F
Sbjct: 121 QISDSQIPLSSGTRLQTLNYIVTVALGGRDSTLIVDTGSDLTWVQCRPCRLCYNQQEPLF 180

Query: 181 DPSNSTSFLSVPCNSTTCSSLV-----YGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFE 240
           DPSNS+SFLS+ CNS TC +L       G C  G GNS+ C YEI+YGDGSYS+G+L FE
Sbjct: 181 DPSNSSSFLSLSCNSPTCLALPPATGNSGLC--GNGNSSSCGYEINYGDGSYSRGELGFE 240

Query: 241 RLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATG 300
           RL LG+I +DNF+ GCG+ N+GL+GG SGLMG  R KLS+VSQTSSVF GIFSYCLP+TG
Sbjct: 241 RLNLGEIVIDNFIFGCGRNNKGLFGGASGLMGLGRSKLSLVSQTSSVFDGIFSYCLPSTG 300

Query: 301 DGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPG 360
            G SGSLTMGGGDFS++RNVSPISYTRM+SNPQMP+FY LN+TGI+IGGV L V    P 
Sbjct: 301 AGASGSLTMGGGDFSNYRNVSPISYTRMVSNPQMPNFYFLNLTGITIGGVNLGV----PN 360

Query: 361 NGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPS 420
           NG  SL+DSGTVITRL PS+Y AFKAEFEKQ  G Q   G+SIL  C+NLT   ++ +P+
Sbjct: 361 NGALSLIDSGTVITRLTPSIYRAFKAEFEKQFSGFQTAPGFSILNTCFNLTGFKEVNIPT 420

Query: 421 VKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQ 480
           VKF+FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQRV+YN++
Sbjct: 421 VKFFFEGNAEMTVDVEGVFYFVKSDASQICLAFASLGYEDQSMIIGNYQQKNQRVVYNSK 480

Query: 481 ESKVGFAMETCSF 489
           ES VGFA E C F
Sbjct: 481 ESTVGFAAEPCGF 485

BLAST of Lcy05g003680 vs. NCBI nr
Match: XP_004135889.2 (aspartyl protease family protein At5g10770 [Cucumis sativus] >KGN45199.1 hypothetical protein Csa_015932 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 9.2e-174
Identity = 312/499 (62.53%), Postives = 383/499 (76.75%), Query Frame = 0

Query: 1   MEIAKSFHSPILLLLLL---LLLFAVDTRSVG---PHNHHHNKKLLLNLRNLPWSQNKKR 60
           MEI+KS H P+ LLLLL   LL   VD RS      +  +H K LL   +N PW ++ + 
Sbjct: 1   MEISKSLHFPLSLLLLLLLPLLSIGVDARSSSFNLGNGDNHEKGLLQLFQNFPWKEHGE- 60

Query: 61  APTDCIFQKPKVGKGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAI 120
           A  +CIFQKPK+ KG TTL+MK+RD CSGK+ DW++  QNR++LDA +V +L S  +SAI
Sbjct: 61  AVVNCIFQKPKITKGITTLEMKQRDYCSGKITDWEKIFQNRIILDAINVNSLFSHFKSAI 120

Query: 121 SPNKPLGISESQIPISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYT 180
            P +   +S+SQIPIS G  LQTLNY+VTVGIGG+N TLIVDTGSD+TWVQC PCR CY 
Sbjct: 121 FPGQTHQLSDSQIPISSGARLQTLNYIVTVGIGGQNSTLIVDTGSDLTWVQCLPCRLCYN 180

Query: 181 QQEPIFDPSNSTSFLSVPCNSTTCSSL-----VYGTCAPGTGNSNPCNYEISYGDGSYSK 240
           QQEP+F+PSNS+SFLS+PCNS TC +L       G C+    NS  C+Y+I YGDGSYS+
Sbjct: 181 QQEPLFNPSNSSSFLSLPCNSPTCVALQPTAGSSGLCS--NKNSTSCDYQIDYGDGSYSR 240

Query: 241 GDLAFERLTLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSY 300
           G+L FE+LTLGK  +DNF+ GCG+ N+GL+GG SGLMG  R +LS+VSQTSS+FG +FSY
Sbjct: 241 GELGFEKLTLGKTEIDNFIFGCGRNNKGLFGGASGLMGLARSELSLVSQTSSLFGSVFSY 300

Query: 301 CLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNV 360
           CLP TG G+SGSLT+GG DFS+F+N+SPISYTRMI NPQM +FY LN+TGISIGGV LNV
Sbjct: 301 CLPTTGVGSSGSLTLGGADFSNFKNISPISYTRMIQNPQMSNFYFLNLTGISIGGVNLNV 360

Query: 361 PRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVT 420
           PRL+   GV SLLDSGTVITRL PS+Y AFKAEFEKQ  G +   G+SIL  C+NLT   
Sbjct: 361 PRLSSNEGVLSLLDSGTVITRLSPSIYKAFKAEFEKQFSGYRTTPGFSILNTCFNLTGYE 420

Query: 421 QMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQR 480
           ++ +P+VKF FEG+A + VDV+G+ YF+KSD+SQ CLAFA L  EDQ  IIGNYQQKNQR
Sbjct: 421 EVNIPTVKFIFEGNAEMIVDVEGVFYFVKSDASQICLAFASLGYEDQTMIIGNYQQKNQR 480

Query: 481 VLYNTQESKVGFAMETCSF 489
           V+YN++ESKVGFA E CSF
Sbjct: 481 VIYNSKESKVGFAGEPCSF 496

BLAST of Lcy05g003680 vs. TAIR 10
Match: AT1G79720.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 461.5 bits (1186), Expect = 8.7e-130
Identity = 236/485 (48.66%), Postives = 324/485 (66.80%), Query Frame = 0

Query: 9   SPILLLLLLLLLFAVDTRSVGPHNHHHNKKLLLNLRNLPWSQNKK-RAPTDCIFQKPKVG 68
           +P+LL+ L LL   V         H  ++K +L++ N  WS  K   A T C  +    G
Sbjct: 12  APLLLVFLFLLSCVV---------HGVDEKKILSVHNNIWSPKKSYEASTSCFSRSLGKG 71

Query: 69  KGATTLQMKERDECSGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKPLGISESQI 128
           + +TTL+MK R+ CSGK  D  + ++  ++LD   V++LQ ++++  S      +SE+QI
Sbjct: 72  RESTTLEMKHRELCSGKTIDLGKKMRRALVLDNIRVQSLQLKIKAMTSSTTEQSVSETQI 131

Query: 129 PISLGQPLQTLNYVVTVGIGGRNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNSTS 188
           P++ G  L++LNY+VTV +GG+N++LIVDTGSD+TWVQC+PCR CY QQ P++DPS S+S
Sbjct: 132 PLTSGIKLESLNYIVTVELGGKNMSLIVDTGSDLTWVQCQPCRSCYNQQGPLYDPSVSSS 191

Query: 189 FLSVPCNSTTCSSLVYGTCAPGTGNSN------PCNYEISYGDGSYSKGDLAFERLTLGK 248
           + +V CNS+TC  LV  T   G    N      PC Y +SYGDGSY++GDLA E + LG 
Sbjct: 192 YKTVFCNSSTCQDLVAATSNSGPCGGNNGVVKTPCEYVVSYGDGSYTRGDLASESILLGD 251

Query: 249 ITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGS 308
             ++NFV GCG+ N+GL+GG+SGLMG  R  +S+VSQT   F G+FSYCLP+  DG SGS
Sbjct: 252 TKLENFVFGCGRNNKGLFGGSSGLMGLGRSSVSLVSQTLKTFNGVFSYCLPSLEDGASGS 311

Query: 309 LTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLAPGNGVTSL 368
           L+  G D S + N + +SYT ++ NPQ+ SFY+LN+TG SIGGV+L       G G+  L
Sbjct: 312 LSF-GNDSSVYTNSTSVSYTPLVQNPQLRSFYILNLTGASIGGVELKSSSF--GRGI--L 371

Query: 369 LDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVKFYFE 428
           +DSGTVITRLPPS+Y A K EF KQ  G     GYSIL+ C+NLT    + +P +K  F+
Sbjct: 372 IDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTCFNLTSYEDISIPIIKMIFQ 431

Query: 429 GDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQESKVGF 487
           G+A L VDV G+ YF+K D+S  CLA A LS E+++GIIGNYQQKNQRV+Y+T + ++G 
Sbjct: 432 GNAELEVDVTGVFYFVKPDASLVCLALASLSYENEVGIIGNYQQKNQRVIYDTTQERLGI 482

BLAST of Lcy05g003680 vs. TAIR 10
Match: AT5G10770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 289.3 bits (739), Expect = 5.9e-78
Identity = 166/397 (41.81%), Postives = 231/397 (58.19%), Query Frame = 0

Query: 98  LDASHVKALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVTVGIG--GRNLTLIV 157
           LD + V ++ S++   ++ +       + +P   G  L + NY+VTVG+G    +L+LI 
Sbjct: 90  LDQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIF 149

Query: 158 DTGSDITWVQCRPC-RKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSN 217
           DTGSD+TW QC+PC R CY Q+EPIF+PS STS+ +V C+S  C SL   T   G+ +++
Sbjct: 150 DTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSAS 209

Query: 218 PCNYEISYGDGSYSKGDLAFERLTLGKITV-DNFVIGCGQTNEGLYGGTSGLMGFDRDKL 277
            C Y I YGD S+S G LA E+ TL    V D    GCG+ N+GL+ G +GL+G  RDKL
Sbjct: 210 NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKL 269

Query: 278 SIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFY 337
           S  SQT++ +  IFSYCLP++   T G LT G    S     +PIS     +     SFY
Sbjct: 270 SFPSQTATAYNKIFSYCLPSSASYT-GHLTFGSAGISRSVKFTPIS-----TITDGTSFY 329

Query: 338 LLNVTGISIGGVKLNVPRL---APGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGL 397
            LN+  I++GG KL +P      PG    +L+DSGTVITRLPP  Y A ++ F+ +    
Sbjct: 330 GLNIVAITVGGQKLPIPSTVFSTPG----ALIDSGTVITRLPPKAYAALRSSFKAKMSKY 389

Query: 398 QKVAGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFAR 457
              +G SIL+ C++L+    + +P V F F G AV+ +  +GI Y  K   SQ CLAFA 
Sbjct: 390 PTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFK--ISQVCLAFAG 449

Query: 458 LSDEDQLGIIGNYQQKNQRVLYNTQESKVGFAMETCS 488
            SD+    I GN QQ+   V+Y+    +VGFA   CS
Sbjct: 450 NSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of Lcy05g003680 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-70
Identity = 149/427 (34.89%), Postives = 227/427 (53.16%), Query Frame = 0

Query: 72  TLQMKERDEC-SGKVRDWDENLQNRVLLDASHVKALQSRVRSAISPNKP--LGISESQIP 131
           TL++  RD   S   R+    L  R+  D   V A+  R+   + P+      +++    
Sbjct: 60  TLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSD 119

Query: 132 ISLGQPLQTLNYVVTVGIGG--RNLTLIVDTGSDITWVQCRPCRKCYTQQEPIFDPSNST 191
           I  G    +  Y V +G+G   R+  +++D+GSD+ WVQC+PC+ CY Q +P+FDP+ S 
Sbjct: 120 IVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSG 179

Query: 192 SFLSVPCNSTTCSSLVYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFERLTLGKITVDN 251
           S+  V C S+ C  +    C     +S  C YE+ YGDGSY+KG LA E LT  K  V N
Sbjct: 180 SYTGVSCGSSVCDRIENSGC-----HSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRN 239

Query: 252 FVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDGTSGSLTMGG 311
             +GCG  N G++ G +GL+G     +S V Q S   GG F YCL + G  ++GSL  G 
Sbjct: 240 VAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG- 299

Query: 312 GDFSSFRNVSPI--SYTRMISNPQMPSFYLLNVTGISIGGVKLNVP-----RLAPGNGVT 371
                 R   P+  S+  ++ NP+ PSFY + + G+ +GGV++ +P         G+G  
Sbjct: 300 ------REALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 359

Query: 372 SLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMKLPSVKFY 431
            ++D+GT +TRLP + Y AF+  F+ Q+  L + +G SI + CY+L+    +++P+V FY
Sbjct: 360 -VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 419

Query: 432 FEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSDEDQLGIIGNYQQKNQRVLYNTQESKV 487
           F    VL +  +  L  +  DS   C AFA  +    L IIGN QQ+  +V ++     V
Sbjct: 420 FTEGPVLTLPARNFLMPV-DDSGTYCFAFA--ASPTGLSIIGNIQQEGIQVSFDGANGFV 470

BLAST of Lcy05g003680 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-65
Identity = 157/435 (36.09%), Postives = 237/435 (54.48%), Query Frame = 0

Query: 72  TLQMKERDECSG-KVRDWDENLQNRVLLDASHVKALQSRVRSAIS--------PNKPLGI 131
           +LQ+  R    G +  D+      R+  D + VK+L +R+  AI+        P   +  
Sbjct: 68  SLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPISTMYT 127

Query: 132 SESQ---IPISLGQPLQTLNYVVTVGIG--GRNLTLIVDTGSDITWVQCRPCRKCYTQQE 191
           +E Q    P+  G    +  Y   VGIG   R + +++DTGSD+ W+QC PC  CY Q E
Sbjct: 128 TEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTE 187

Query: 192 PIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSNPCNYEISYGDGSYSKGDLAFERL 251
           PIF+PS+S+S+  + C++  C++L    C   T     C YE+SYGDGSY+ GD A E L
Sbjct: 188 PIFEPSSSSSYEPLSCDTPQCNALEVSECRNAT-----CLYEVSYGDGSYTVGDFATETL 247

Query: 252 TLGKITVDNFVIGCGQTNEGLYGGTSGLMGFDRDKLSIVSQTSSVFGGIFSYCLPATGDG 311
           T+G   V N  +GCG +NEGL+ G +GL+G     L++ SQ ++     FSYCL    D 
Sbjct: 248 TIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCL-VDRDS 307

Query: 312 TSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYLLNVTGISIGGVKLNVPRLA---- 371
            S S    G   S    V+P     ++ N Q+ +FY L +TGIS+GG  L +P+ +    
Sbjct: 308 DSASTVDFGTSLSPDAVVAP-----LLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMD 367

Query: 372 -PGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKVAGYSILEACYNLTEVTQMK 431
             G+G   ++DSGT +TRL   +Y++ +  F K +  L+K AG ++ + CYNL+  T ++
Sbjct: 368 ESGSG-GIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVE 427

Query: 432 LPSVKFYFEGDAVLNVDVQGILYFLKSDSSQT-CLAFARLSDEDQLGIIGNYQQKNQRVL 487
           +P+V F+F G  +L +  +   Y +  DS  T CLAFA  +    L IIGN QQ+  RV 
Sbjct: 428 VPTVAFHFPGGKMLALPAKN--YMIPVDSVGTFCLAFAPTA--SSLAIIGNVQQQGTRVT 483

BLAST of Lcy05g003680 vs. TAIR 10
Match: AT5G10760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.4e-63
Identity = 144/393 (36.64%), Postives = 221/393 (56.23%), Query Frame = 0

Query: 99  DASHVKALQSRVRSAISPNKPLGISESQIPISLGQPLQTLNYVVTVGIG--GRNLTLIVD 158
           D + V+++ S++ S  S N+      +++P   G  L + NY+VT+GIG    +L+L+ D
Sbjct: 92  DQARVESIYSKL-SKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFD 151

Query: 159 TGSDITWVQCRPC-RKCYTQQEPIFDPSNSTSFLSVPCNSTTCSSLVYGTCAPGTGNSNP 218
           TGSD+TW QC PC   CY+Q+EP F+PS+S+++ +V C+S  C        A     SN 
Sbjct: 152 TGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED------AESCSASN- 211

Query: 219 CNYEISYGDGSYSKGDLAFERLTLGKITV-DNFVIGCGQTNEGLYGGTSGLMGFDRDKLS 278
           C Y I YGD S+++G LA E+ TL    V ++   GCG+ N+GL+ G +GL+G    KLS
Sbjct: 212 CVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLS 271

Query: 279 IVSQTSSVFGGIFSYCLPATGDGTSGSLTMGGGDFSSFRNVSPISYTRMISNPQMPSFYL 338
           + +QT++ +  IFSYCLP+    ++G LT G    S     +PIS      N      Y 
Sbjct: 272 LPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN------YG 331

Query: 339 LNVTGISIGGVKLNV-PRLAPGNGVTSLLDSGTVITRLPPSVYDAFKAEFEKQSPGLQKV 398
           +++ GIS+G  +L + P      G  +++DSGTV TRLP  VY   ++ F+++    +  
Sbjct: 332 IDIIGISVGDKELAITPNSFSTEG--AIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKST 391

Query: 399 AGYSILEACYNLTEVTQMKLPSVKFYFEGDAVLNVDVQGILYFLKSDSSQTCLAFARLSD 458
           +GY + + CY+ T +  +  P++ F F G  V+ +D  GI   +K   SQ CLAFA   +
Sbjct: 392 SGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIK--ISQVCLAFA--GN 451

Query: 459 EDQLGIIGNYQQKNQRVLYNTQESKVGFAMETC 487
           +D   I GN QQ    V+Y+    +VGFA   C
Sbjct: 452 DDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S9J68.3e-7741.81Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LHE38.3e-6934.89Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LEW33.4e-6236.64Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q9LNJ31.0e-5834.13Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS406.6e-5831.58Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A6J1JS166.8e-17563.89aspartyl protease family protein At5g10770 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0K8J24.4e-17462.53Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G43132... [more]
A0A1S3CDQ05.8e-17462.98aspartyl protease family protein At5g10770 OS=Cucumis melo OX=3656 GN=LOC1034998... [more]
A0A6J1GQV93.8e-17363.49aspartyl protease family protein At5g10770 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5A7UYY61.9e-17262.73Aspartyl protease family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
KAG6575642.11.5e-17664.98Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7014194.13.4e-17664.50Aspartyl protease family protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023547381.11.3e-17564.36aspartyl protease family protein At5g10770 [Cucurbita pepo subsp. pepo][more]
XP_022991886.11.4e-17463.89aspartyl protease family protein At5g10770 [Cucurbita maxima][more]
XP_004135889.29.2e-17462.53aspartyl protease family protein At5g10770 [Cucumis sativus] >KGN45199.1 hypothe... [more]
Match NameE-valueIdentityDescription
AT1G79720.18.7e-13048.66Eukaryotic aspartyl protease family protein [more]
AT5G10770.15.9e-7841.81Eukaryotic aspartyl protease family protein [more]
AT3G20015.15.9e-7034.89Eukaryotic aspartyl protease family protein [more]
AT1G25510.11.2e-6536.09Eukaryotic aspartyl protease family protein [more]
AT5G10760.12.4e-6336.64Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 360..371
score: 32.32
coord: 300..313
score: 32.03
coord: 144..164
score: 40.43
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 13..487
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 121..305
e-value: 1.5E-46
score: 160.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 310..488
e-value: 3.5E-42
score: 146.2
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 135..486
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 333..482
e-value: 1.2E-27
score: 96.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 140..306
e-value: 9.6E-47
score: 159.5
NoneNo IPR availablePANTHERPTHR13683:SF827SUBFAMILY NOT NAMEDcoord: 13..487
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 153..164
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 140..482
score: 41.89922

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g003680.1Lcy05g003680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity