Lcy05g003350 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g003350
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionF-box/LRR-repeat protein 14
LocationChr05: 2995483 .. 2997484 (+)
RNA-Seq ExpressionLcy05g003350
SyntenyLcy05g003350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTCATCGAGTTTTGGCTATGTCTGTCTGATCTTTGCTTTGATCATAGTGCTAATGTTGTCTGAGAGACCTGCAAACAATTTCGGGATAACTGATTTCGAGTTATTATACTGTTTTTGTAGTTTTTGCTCACCTCCCTACGAAATCTTAGCTAATGGATGTTATCTTGTGTTGCAGAAGTGCCTTGTTATAATGGATAGTTTACCAGAAGTATTGATTTGGGAGATATTGAACAGAGTTAAGAAAACCATTGATAGAAACTCTTTGGCATTATCATGTAAGCGCCTTCACGGTTTGGACAAAGAAAACAGACACTTTCTTCGAGTTGGTTGTGGATTGGACCCTGCAGATGAAGCTCTGACATCTCTGTGCCTCAGGTTTCCCAATTTAGGACGAATCGAGATCACGTATTCGGGTTGGATGTCAAAACTAGGGAAGCAATTGGATGATCATGGGCTTTTCATTCTCTCTAACCATTGTCCATCTCTCACCGATCTCACCTTAAGCTATTGCACGTTCATCACTGATGTTGGACTTCGCCATTTAACTTCCTGCTACAAGCTTTCAGCTCTAAAGCTGAACTTCACCCCGAGAATCACCGGCTGTGGCATATTCTCAATCGCTGTTGGATGCAAAAACCTCACCATTCTCCATCTTATTCGATGTTTGAATGTCAGCAGTGTTGAGTGGCTTGAATACCTCGGCAAGCTTGAAACACTCGAAGATCTCTCGATCAGGAATTGTAGAGCAATTGGTGAAGGTGATCTGATCAAGCTAGGCCATAGCTGGCGAAAGTTGAAGCGGCTGCAATTCGAAGTTGATGCTAACTACCGATACATGAAGGTTTATGATCGTTTAGCTGTTGATCGATGGCAAAAGCAGTGGATCCCATGTGATGATATGCTGGAACTTAGCTTGGTGAATTGCATTATCAGTCCAGGGAGAGGACTTGCCTGTGTGTTGGGAAAGTGCAAGAACTTGCAGAAAATTCACTTAGATATGTGTGTTGGAGTTAGAGATTGTGATATAATAAGCTTGGCTCGAGAATCGCATAATCTCCGCTCCATTTCCCTCCGAGTTCCATCAGATTTTTCGCTTCCATTGTTAGCCAACAACACGCTGAGATTAACGGACGAGAGCTTAAAAGCCTTAGCCGAGAACTGCTCCCACCTTGAGTCAGTTAGAATCTCATTTGCAGATGGTGAATTCCCCTCACTATCTTCATTTTCCCTCAATGGGATCCTTGTTCTAGTTCACAAATGCCCCGTTCGAGAGCTCTCCCTCGACCATGTCTACTCCTTCAACGACGTGGGATTGGAAGCTCTCTGCTCGGCCTCCTATCTCGAAACTCTAGAGCTCGTAAGATGCCAAGAGATAAGTGATGAGGGGCTGCAGCTAGTGAGCCAATTCCCTCAGCTACAAAACCTACGGCTGAGCAAATGCTTGGGGATCACCGACGATGGGCTGAAGCCGCTGGTCGATGCATACAACAAACTCGAATCATTGGTCGTGGAAGACTGCCCTCAGATCTCCGAGAGGGGCGTCCATGGCGCCGCAAGAACCATATCATTCAGGCAAGACCTGTCATGGATGTATTGAAACATGCTGAGTTTCCATGTCACATCAACAGGTGAGTGATTTAGTATAATGAATGTAACATGTAAGTTCCCAAATGTATAACAAATTATGGACTCATTCATTTGTTAAATAATTTGCTTCTGCTTCATGTTTGTAAATTATGTTTAAATTATGTTTGAGTTTGGCCTGTCTTATTTCTTTTTTATTTTTTTGGCTTTGTTAATTGTCTCATAGTGGTTGTTTTGGGCAGTCGTCTGACGTGGAGTTGATGCTGACTGTAAACTCCATGTCACCTCATGCATCATGTATCATTGTTCCGCTTCCAGCGCCCCATGGTCCCAATACAACAATACTTTTTTATTTTGGCCTCAAACGTCATGTCGCACTGGAGACTCCCATTTGTTGACGAAAGTACCCCTCTAGA

mRNA sequence

TTGTCATCGAGTTTTGGCTATGTCTGTCTGATCTTTGCTTTGATCATAGTGCTAATGTTGTCTGAGAGACCTGCAAACAATTTCGGGATAACTGATTTCGAGTTATTATACTGTTTTTGTAGTTTTTGCTCACCTCCCTACGAAATCTTAGCTAATGGATGTTATCTTGTGTTGCAGAAGTGCCTTGTTATAATGGATAGTTTACCAGAAGTATTGATTTGGGAGATATTGAACAGAGTTAAGAAAACCATTGATAGAAACTCTTTGGCATTATCATGTAAGCGCCTTCACGGTTTGGACAAAGAAAACAGACACTTTCTTCGAGTTGGTTGTGGATTGGACCCTGCAGATGAAGCTCTGACATCTCTGTGCCTCAGGTTTCCCAATTTAGGACGAATCGAGATCACGTATTCGGGTTGGATGTCAAAACTAGGGAAGCAATTGGATGATCATGGGCTTTTCATTCTCTCTAACCATTGTCCATCTCTCACCGATCTCACCTTAAGCTATTGCACGTTCATCACTGATGTTGGACTTCGCCATTTAACTTCCTGCTACAAGCTTTCAGCTCTAAAGCTGAACTTCACCCCGAGAATCACCGGCTGTGGCATATTCTCAATCGCTGTTGGATGCAAAAACCTCACCATTCTCCATCTTATTCGATGTTTGAATGTCAGCAGTGTTGAGTGGCTTGAATACCTCGGCAAGCTTGAAACACTCGAAGATCTCTCGATCAGGAATTGTAGAGCAATTGGTGAAGGTGATCTGATCAAGCTAGGCCATAGCTGGCGAAAGTTGAAGCGGCTGCAATTCGAAGTTGATGCTAACTACCGATACATGAAGGTTTATGATCGTTTAGCTGTTGATCGATGGCAAAAGCAGTGGATCCCATGTGATGATATGCTGGAACTTAGCTTGGTGAATTGCATTATCAGTCCAGGGAGAGGACTTGCCTGTGTGTTGGGAAAGTGCAAGAACTTGCAGAAAATTCACTTAGATATGTGTGTTGGAGTTAGAGATTGTGATATAATAAGCTTGGCTCGAGAATCGCATAATCTCCGCTCCATTTCCCTCCGAGTTCCATCAGATTTTTCGCTTCCATTGTTAGCCAACAACACGCTGAGATTAACGGACGAGAGCTTAAAAGCCTTAGCCGAGAACTGCTCCCACCTTGAGTCAGTTAGAATCTCATTTGCAGATGGTGAATTCCCCTCACTATCTTCATTTTCCCTCAATGGGATCCTTGTTCTAGTTCACAAATGCCCCGTTCGAGAGCTCTCCCTCGACCATGTCTACTCCTTCAACGACGTGGGATTGGAAGCTCTCTGCTCGGCCTCCTATCTCGAAACTCTAGAGCTCGTAAGATGCCAAGAGATAAGTGATGAGGGGCTGCAGCTAGTGAGCCAATTCCCTCAGCTACAAAACCTACGGCTGAGCAAATGCTTGGGGATCACCGACGATGGGCTGAAGCCGCTGGTCGATGCATACAACAAACTCGAATCATTGGTCGTGGAAGACTGCCCTCAGATCTCCGAGAGGGGCGTCCATGGCGCCGCAAGAACCATATCATTCAGGCAAGACCTGTCATGGATGTATTGAAACATGCTGAGTTTCCATGTCACATCAACAGTCGTCTGACGTGGAGTTGATGCTGACTGTAAACTCCATGTCACCTCATGCATCATGTATCATTGTTCCGCTTCCAGCGCCCCATGGTCCCAATACAACAATACTTTTTTATTTTGGCCTCAAACGTCATGTCGCACTGGAGACTCCCATTTGTTGACGAAAGTACCCCTCTAGA

Coding sequence (CDS)

ATGTTGTCTGAGAGACCTGCAAACAATTTCGGGATAACTGATTTCGAGTTATTATACTGTTTTTGTAGTTTTTGCTCACCTCCCTACGAAATCTTAGCTAATGGATGTTATCTTGTGTTGCAGAAGTGCCTTGTTATAATGGATAGTTTACCAGAAGTATTGATTTGGGAGATATTGAACAGAGTTAAGAAAACCATTGATAGAAACTCTTTGGCATTATCATGTAAGCGCCTTCACGGTTTGGACAAAGAAAACAGACACTTTCTTCGAGTTGGTTGTGGATTGGACCCTGCAGATGAAGCTCTGACATCTCTGTGCCTCAGGTTTCCCAATTTAGGACGAATCGAGATCACGTATTCGGGTTGGATGTCAAAACTAGGGAAGCAATTGGATGATCATGGGCTTTTCATTCTCTCTAACCATTGTCCATCTCTCACCGATCTCACCTTAAGCTATTGCACGTTCATCACTGATGTTGGACTTCGCCATTTAACTTCCTGCTACAAGCTTTCAGCTCTAAAGCTGAACTTCACCCCGAGAATCACCGGCTGTGGCATATTCTCAATCGCTGTTGGATGCAAAAACCTCACCATTCTCCATCTTATTCGATGTTTGAATGTCAGCAGTGTTGAGTGGCTTGAATACCTCGGCAAGCTTGAAACACTCGAAGATCTCTCGATCAGGAATTGTAGAGCAATTGGTGAAGGTGATCTGATCAAGCTAGGCCATAGCTGGCGAAAGTTGAAGCGGCTGCAATTCGAAGTTGATGCTAACTACCGATACATGAAGGTTTATGATCGTTTAGCTGTTGATCGATGGCAAAAGCAGTGGATCCCATGTGATGATATGCTGGAACTTAGCTTGGTGAATTGCATTATCAGTCCAGGGAGAGGACTTGCCTGTGTGTTGGGAAAGTGCAAGAACTTGCAGAAAATTCACTTAGATATGTGTGTTGGAGTTAGAGATTGTGATATAATAAGCTTGGCTCGAGAATCGCATAATCTCCGCTCCATTTCCCTCCGAGTTCCATCAGATTTTTCGCTTCCATTGTTAGCCAACAACACGCTGAGATTAACGGACGAGAGCTTAAAAGCCTTAGCCGAGAACTGCTCCCACCTTGAGTCAGTTAGAATCTCATTTGCAGATGGTGAATTCCCCTCACTATCTTCATTTTCCCTCAATGGGATCCTTGTTCTAGTTCACAAATGCCCCGTTCGAGAGCTCTCCCTCGACCATGTCTACTCCTTCAACGACGTGGGATTGGAAGCTCTCTGCTCGGCCTCCTATCTCGAAACTCTAGAGCTCGTAAGATGCCAAGAGATAAGTGATGAGGGGCTGCAGCTAGTGAGCCAATTCCCTCAGCTACAAAACCTACGGCTGAGCAAATGCTTGGGGATCACCGACGATGGGCTGAAGCCGCTGGTCGATGCATACAACAAACTCGAATCATTGGTCGTGGAAGACTGCCCTCAGATCTCCGAGAGGGGCGTCCATGGCGCCGCAAGAACCATATCATTCAGGCAAGACCTGTCATGGATGTATTGA

Protein sequence

MLSERPANNFGITDFELLYCFCSFCSPPYEILANGCYLVLQKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY
Homology
BLAST of Lcy05g003350 vs. ExPASy Swiss-Prot
Match: Q3EC97 (F-box/LRR-repeat protein 14 OS=Arabidopsis thaliana OX=3702 GN=FBL14 PE=2 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.1e-203
Identity = 337/468 (72.01%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MD LP+ L+W+IL+++  T DRNSL+LSCKR   LD E R+ LR+GCGL PA +AL SLC
Sbjct: 14  MDELPDHLVWDILSKLHTTDDRNSLSLSCKRFFSLDNEQRYSLRIGCGLVPASDALLSLC 73

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
            RFPNL ++EI YSGWMSKLGKQ+DD GL +L+ +C SLTDLTLS+CTFITDVG+ HL+S
Sbjct: 74  RRFPNLSKVEIIYSGWMSKLGKQVDDQGLLVLTTNCHSLTDLTLSFCTFITDVGIGHLSS 133

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           C +LS+LKLNF PRITGCG+ S+AVGCK L  LHLIRCLNV+SVEWLEY GKLETLE+L 
Sbjct: 134 CPELSSLKLNFAPRITGCGVLSLAVGCKKLRRLHLIRCLNVASVEWLEYFGKLETLEELC 193

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           I+NCRAIGEGDLIKL +SWRKL  LQFEVDANYRYMKVYD+L V+RW KQ +PCD ++EL
Sbjct: 194 IKNCRAIGEGDLIKLRNSWRKLTSLQFEVDANYRYMKVYDQLDVERWPKQLVPCDSLVEL 253

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SL NCII+PGRGLACVL  CKNL+K+HLDMC GV D DII+L +++ +LRSISLRVPSDF
Sbjct: 254 SLGNCIIAPGRGLACVLRNCKNLEKLHLDMCTGVSDSDIIALVQKASHLRSISLRVPSDF 313

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           +LPLL N TLRLTDESL A+A++CS LES +ISF+DGEFPSL SF+L GI+ L+ KCPVR
Sbjct: 314 TLPLLNNITLRLTDESLSAIAQHCSKLESFKISFSDGEFPSLFSFTLQGIITLIQKCPVR 373

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           ELSLDHV  FND+G+EALCSA  LE LELV CQE+SDEGL LVSQFP L  L+LSKCLG+
Sbjct: 374 ELSLDHVCVFNDMGMEALCSAQKLEILELVHCQEVSDEGLILVSQFPSLNVLKLSKCLGV 433

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDG++PLV ++ KLE LVVEDCPQ+S RGVHGAA ++SF+QDLSWMY
Sbjct: 434 TDDGMRPLVGSH-KLELLVVEDCPQVSRRGVHGAATSVSFKQDLSWMY 480

BLAST of Lcy05g003350 vs. ExPASy Swiss-Prot
Match: Q8RWU5 (F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-25
Identity = 138/563 (24.51%), Postives = 224/563 (39.79%), Query Frame = 0

Query: 48  DSLPEVLIWEILNRVK-KTIDRNSLALSCKRLHGLDKENRHFL---------------RV 107
           D L E L++ IL+ +     D  S +L+CK  + L+ ++R  L               R 
Sbjct: 14  DLLSEELVFIILDLISPNPSDLKSFSLTCKSFYQLESKHRGSLKPLRSDYLPRILTRYRN 73

Query: 108 GCGLD------PADEALTSL-CLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPS 167
              LD        D AL+ + CL  P L  ++++ SG  S         GL  L+  C +
Sbjct: 74  TTDLDLTFCPRVTDYALSVVGCLSGPTLRSLDLSRSGSFSAA-------GLLRLALKCVN 133

Query: 168 LTDLTLSYCTFITDVGLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHL--- 227
           L ++ LS  T + D     +     L  LKL     +T  GI  IAVGCK L  + L   
Sbjct: 134 LVEIDLSNATEMRDADAAVVAEARSLERLKLGRCKMLTDMGIGCIAVGCKKLNTVSLKWC 193

Query: 228 ------------IRCLNVSSVEWLEYLG----------KLETLEDLSIRNCRAIGEGDLI 287
                       ++C ++ +++ L YL           KL+ LE+L +  C  + +  L 
Sbjct: 194 VGVGDLGVGLLAVKCKDIRTLD-LSYLPITGKCLHDILKLQHLEELLLEGCFGVDDDSLK 253

Query: 288 KLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ--WIPCDDMLELS---------- 347
            L H  + LK+L      N  +  +   L+   + ++     C  ++ L           
Sbjct: 254 SLRHDCKSLKKLDASSCQNLTHRGLTSLLSGAGYLQRLDLSHCSSVISLDFASSLKKVSA 313

Query: 348 -----LVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISL-- 407
                L  C ++P  GL  +   C +L+++ L  CV V D  + SL  +  +LR + +  
Sbjct: 314 LQSIRLDGCSVTPD-GLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDITC 373

Query: 408 -RVPSDFSLPLLANNTLRLTD-----------ESLKALAENCSHLESVRISFADGEFP-- 467
            R  S  S+  +AN+   L             E+   + + C  LE   +   D E    
Sbjct: 374 CRKLSRVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKCRLLE--ELDLTDNEIDDE 433

Query: 468 ---------SLSSFSLNGILVLVHK---------CPVRELSLDHVYSFNDVGLEALCSAS 511
                    SLSS  L   L +  K           +REL L       DVG+  +    
Sbjct: 434 GLKSISSCLSLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVGISTIAQGC 493

BLAST of Lcy05g003350 vs. ExPASy Swiss-Prot
Match: Q9C5D2 (F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.9e-19
Identity = 95/381 (24.93%), Postives = 159/381 (41.73%), Query Frame = 0

Query: 130 LDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS-CYKLSALKLNFTPRITGCGIFS 189
           + D GL  ++  C  L +L L  C  +TDV    +   C  L  L L      T  G+ +
Sbjct: 256 IHDKGLIAVAQGCHRLKNLKLQ-CVSVTDVAFAAVGELCTSLERLALYSFQHFTDKGMRA 315

Query: 190 IAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIKLGHSWRKL 249
           I  G K L  L L  C  VS           + LE + I  C  IG   +  +G S  +L
Sbjct: 316 IGKGSKKLKDLTLSDCYFVSCKGLEAIAHGCKELERVEINGCHNIGTRGIEAIGKSCPRL 375

Query: 250 KRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLACVLGKCKN 309
           K L          +    R+     Q+    C  +  L LV+C       +  +   C+N
Sbjct: 376 KELA---------LLYCQRIGNSALQEIGKGCKSLEILHLVDCSGIGDIAMCSIAKGCRN 435

Query: 310 LQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTDESLKALAE 369
           L+K+H+  C  + +  IIS+ +   +L  +SLR               ++ +++L A+ +
Sbjct: 436 LKKLHIRRCYEIGNKGIISIGKHCKSLTELSLRFCD------------KVGNKALIAIGK 495

Query: 370 NCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCP-VRELSLDHVYSFNDVGLEALC-S 429
            CS L+ + +S         +  S  GI  +   CP +  L +  + +  D+ L  L   
Sbjct: 496 GCS-LQQLNVS-------GCNQISDAGITAIARGCPQLTHLDISVLQNIGDMPLAELGEG 555

Query: 430 ASYLETLELVRCQEISDEGL-QLVSQFPQLQNLRLSKCLGITDDGLKPLVDAYNKLESLV 489
              L+ L L  C  I+D GL  LV +   L+   +  C GIT  G+  +V +   ++ ++
Sbjct: 556 CPMLKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITSAGVATVVSSCPHIKKVL 605

Query: 490 VEDCPQISERGVHGAARTISF 507
           +E   +++ER    A   IS+
Sbjct: 616 IEKW-KVTERTTRRAGSVISY 605

BLAST of Lcy05g003350 vs. ExPASy Swiss-Prot
Match: A6H779 (F-box/LRR-repeat protein 2 OS=Bos taurus OX=9913 GN=FBXL2 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.3e-15
Identity = 69/279 (24.73%), Postives = 120/279 (43.01%), Query Frame = 0

Query: 222 LEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCD 281
           L  LS+R C  +G+  L     + R ++ L             Y   ++ R+      C 
Sbjct: 80  LRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCY---SLSRF------CS 139

Query: 282 DMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLR 341
            +  L L +C+      L  +   C++L+ ++L  C  +    + +L R    LR++ LR
Sbjct: 140 KLKHLDLTSCVSITNSSLKGISEGCRHLEYLNLSWCDQITKDGVEALVRGCRGLRALLLR 199

Query: 342 VPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVH 401
                          +L DE+LK +   C  L S+ +        S S  + +G++ L  
Sbjct: 200 ------------GCTQLEDEALKHIQNYCHELVSLNLQ-------SCSRVTDDGVVQLCR 259

Query: 402 KCP-VRELSLDHVYSFNDVGLEALC-SASYLETLELVRCQEISDEGLQLVSQ-FPQLQNL 461
            CP ++ L L    S  D  L AL  +   L+ LE  RC  ++D G  L+++    L+ +
Sbjct: 260 GCPRLQALCLSGCGSLTDASLTALALNCPRLQILEAARCSHLTDAGFTLLARNCHDLEKM 319

Query: 462 RLSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGV 498
            L +C+ ITD  L  L     KL++L +  C  I++ G+
Sbjct: 320 DLEECILITDRTLTQLSIHCPKLQALSLSHCELITDDGI 330

BLAST of Lcy05g003350 vs. ExPASy Swiss-Prot
Match: Q58DG6 (F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 4.3e-15
Identity = 83/343 (24.20%), Postives = 137/343 (39.94%), Query Frame = 0

Query: 138 LSNHCPS-LTDLTLSYCTFITDVGLRHLT-SCYKLSALKLNFTPRITGCGIFSIAVGCKN 197
           +S  C   L  L+L  C  + D  LR    +C  +  L LN   + T     S++  C  
Sbjct: 85  ISKRCGGFLRKLSLRGCLGVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSKFCSK 144

Query: 198 LTILHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEV 257
           L  L L  C +++++           LE L+I  C  + +  +  L      LK L    
Sbjct: 145 LRHLDLASCTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKAL---- 204

Query: 258 DANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLD 317
                ++K   +L  +  +     C +++ L+L  C+     GL  +   C  LQ +   
Sbjct: 205 -----FLKGCTQLEDEALKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCAS 264

Query: 318 MCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLES 377
            C  + D  + +L +    LR + +   S            +LTD     LA NC  LE 
Sbjct: 265 GCSNITDAILNALGQNCPRLRILEVARCS------------QLTDVGFTTLARNCHELEK 324

Query: 378 VRISFADGEFPSLSSFSLNGILVLVHKCPVRELSLDHVYSFNDVGLEAL----CSASYLE 437
           + +     E   ++  +L  I + +H   ++ LSL H     D G+  L    C+   LE
Sbjct: 325 MDLE----ECVQITDSTL--IQLSIHCPRLQVLSLSHCELITDDGIRHLGNGACAHDQLE 384

Query: 438 TLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGITDDGLKPL 475
            +EL  C  I+D  L+ +     L+ + L  C  IT  G+K L
Sbjct: 385 VIELDNCPLITDASLEHLKSCHSLERIELYDCQQITRAGIKRL 400

BLAST of Lcy05g003350 vs. ExPASy TrEMBL
Match: A0A6J1DK86 (F-box/LRR-repeat protein 14 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021310 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.6e-269
Identity = 467/514 (90.86%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 1   MLSERPANNFGITDFELLYCFCSFCSPPYEILANGCYLVLQKCLVIMDSLPEVLIWEILN 60
           M SE    +FGI  F L   F  F      +L + C LVLQK LV MD+LPEVLIWEILN
Sbjct: 1   MWSESHGEDFGI--FRLFIQFLQF------LLIDYC-LVLQKRLVTMDALPEVLIWEILN 60

Query: 61  RVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLCLRFPNLGRIEITYS 120
           RVKKT+DRNSL+LSCKRLH LDKENR FLRVGCGLDPADEALTSLCLRFPNL RIEITYS
Sbjct: 61  RVKKTVDRNSLSLSCKRLHDLDKENRQFLRVGCGLDPADEALTSLCLRFPNLSRIEITYS 120

Query: 121 GWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTSCYKLSALKLNFTPR 180
           GWMSKLGKQLDDHGLFILS+HCPSLTDLTLSYCTFITDVGLRHLTSC  LSALKLNFTPR
Sbjct: 121 GWMSKLGKQLDDHGLFILSSHCPSLTDLTLSYCTFITDVGLRHLTSCSNLSALKLNFTPR 180

Query: 181 ITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK 240
           ITGCG+FSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK
Sbjct: 181 ITGCGVFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK 240

Query: 241 LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLA 300
           LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPG+GLA
Sbjct: 241 LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGKGLA 300

Query: 301 CVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD 360
           CVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD
Sbjct: 301 CVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD 360

Query: 361 ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVRELSLDHVYSFNDVG 420
           ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVH CPVREL+LDHVYSFND+G
Sbjct: 361 ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHMCPVRELALDHVYSFNDMG 420

Query: 421 LEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGITDDGLKPLVDAYNK 480
           LEALCSASYLETLELVRCQEISDEGLQLVSQFPQL+ LRLSKCLGITDDGLKPLVDAY K
Sbjct: 421 LEALCSASYLETLELVRCQEISDEGLQLVSQFPQLRYLRLSKCLGITDDGLKPLVDAY-K 480

Query: 481 LESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           LESL VEDCPQISERGVHGAARTISFRQDLSWMY
Sbjct: 481 LESLAVEDCPQISERGVHGAARTISFRQDLSWMY 504

BLAST of Lcy05g003350 vs. ExPASy TrEMBL
Match: A0A6J1H5R5 (F-box/LRR-repeat protein 14 OS=Cucurbita moschata OX=3662 GN=LOC111460733 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.5e-265
Identity = 449/468 (95.94%), Postives = 462/468 (98.72%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC
Sbjct: 1   MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 60

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
           LRFPNL RIEITYSGWMSKLGKQLDD GLFILSNHCP LTDLTLSYCTFITDVGLRHLTS
Sbjct: 61  LRFPNLTRIEITYSGWMSKLGKQLDDRGLFILSNHCPCLTDLTLSYCTFITDVGLRHLTS 120

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           CYKLS+LKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKL TLEDLS
Sbjct: 121 CYKLSSLKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLGTLEDLS 180

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWI CDDMLEL
Sbjct: 181 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWILCDDMLEL 240

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISLRVPSDF
Sbjct: 241 SLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 300

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           SLPLLA+NTLRLTDESLKALA+NC+HLESVRISFADGEFPSLSSFSLNGILVLVHKCP++
Sbjct: 301 SLPLLAHNTLRLTDESLKALADNCAHLESVRISFADGEFPSLSSFSLNGILVLVHKCPIQ 360

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           EL+LDHVYSFND+GLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI
Sbjct: 361 ELALDHVYSFNDMGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 420

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDGLKPLVDAY KLESL+VEDCPQISERG+HGAAR+ISFRQDLSWMY
Sbjct: 421 TDDGLKPLVDAY-KLESLIVEDCPQISERGIHGAARSISFRQDLSWMY 467

BLAST of Lcy05g003350 vs. ExPASy TrEMBL
Match: A0A5A7UYS9 (F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001950 PE=4 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.2e-264
Identity = 454/478 (94.98%), Postives = 462/478 (96.65%), Query Frame = 0

Query: 37  YLVLQKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLD 96
           YL+ QK LV MDSLPEVLIWEILNRVKKT+DRNSLALSCKR H LDKENR FLRVGCGLD
Sbjct: 4   YLMTQKFLVRMDSLPEVLIWEILNRVKKTVDRNSLALSCKRFHRLDKENRQFLRVGCGLD 63

Query: 97  PADEALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI 156
           PADEALTSLCLRFPNL RIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI
Sbjct: 64  PADEALTSLCLRFPNLVRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI 123

Query: 157 TDVGLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYL 216
           TDVGLR+LTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEY 
Sbjct: 124 TDVGLRNLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYF 183

Query: 217 GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ 276
           GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ
Sbjct: 184 GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ 243

Query: 277 WIPCDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLR 336
           WI CDDMLELSLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARES NLR
Sbjct: 244 WISCDDMLELSLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESRNLR 303

Query: 337 SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI 396
           SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI
Sbjct: 304 SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI 363

Query: 397 LVLVHKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQ 456
           LVLV  CPVRELSLDHVYSFND+GLEALCSASYLE+LELVRCQEISD+GLQLVSQFPQLQ
Sbjct: 364 LVLVRMCPVRELSLDHVYSFNDMGLEALCSASYLESLELVRCQEISDDGLQLVSQFPQLQ 423

Query: 457 NLRLSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           NLRLSKCLGITDDGLK LVD Y KLESLVVEDCPQISERGVHGAAR ISFRQDLSWMY
Sbjct: 424 NLRLSKCLGITDDGLKLLVDTY-KLESLVVEDCPQISERGVHGAARAISFRQDLSWMY 480

BLAST of Lcy05g003350 vs. ExPASy TrEMBL
Match: A0A6J1DM45 (F-box/LRR-repeat protein 14 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021310 PE=4 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.2e-264
Identity = 448/468 (95.73%), Postives = 459/468 (98.08%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MD+LPEVLIWEILNRVKKT+DRNSL+LSCKRLH LDKENR FLRVGCGLDPADEALTSLC
Sbjct: 1   MDALPEVLIWEILNRVKKTVDRNSLSLSCKRLHDLDKENRQFLRVGCGLDPADEALTSLC 60

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
           LRFPNL RIEITYSGWMSKLGKQLDDHGLFILS+HCPSLTDLTLSYCTFITDVGLRHLTS
Sbjct: 61  LRFPNLSRIEITYSGWMSKLGKQLDDHGLFILSSHCPSLTDLTLSYCTFITDVGLRHLTS 120

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           C  LSALKLNFTPRITGCG+FSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKLETLEDLS
Sbjct: 121 CSNLSALKLNFTPRITGCGVFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLETLEDLS 180

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL
Sbjct: 181 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 240

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SLVNCIISPG+GLACVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISLRVPSDF
Sbjct: 241 SLVNCIISPGKGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 300

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVH CPVR
Sbjct: 301 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHMCPVR 360

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           EL+LDHVYSFND+GLEALCSASYLETLELVRCQEISDEGLQLVSQFPQL+ LRLSKCLGI
Sbjct: 361 ELALDHVYSFNDMGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLRYLRLSKCLGI 420

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDGLKPLVDAY KLESL VEDCPQISERGVHGAARTISFRQDLSWMY
Sbjct: 421 TDDGLKPLVDAY-KLESLAVEDCPQISERGVHGAARTISFRQDLSWMY 467

BLAST of Lcy05g003350 vs. ExPASy TrEMBL
Match: A0A1S3CEI1 (F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499827 PE=4 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.2e-264
Identity = 454/478 (94.98%), Postives = 462/478 (96.65%), Query Frame = 0

Query: 37  YLVLQKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLD 96
           YL+ QK LV MDSLPEVLIWEILNRVKKT+DRNSLALSCKR H LDKENR FLRVGCGLD
Sbjct: 4   YLMTQKFLVRMDSLPEVLIWEILNRVKKTVDRNSLALSCKRFHRLDKENRQFLRVGCGLD 63

Query: 97  PADEALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI 156
           PADEALTSLCLRFPNL RIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI
Sbjct: 64  PADEALTSLCLRFPNLVRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFI 123

Query: 157 TDVGLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYL 216
           TDVGLR+LTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEY 
Sbjct: 124 TDVGLRNLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYF 183

Query: 217 GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ 276
           GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ
Sbjct: 184 GKLETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ 243

Query: 277 WIPCDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLR 336
           WI CDDMLELSLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARES NLR
Sbjct: 244 WISCDDMLELSLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESRNLR 303

Query: 337 SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI 396
           SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI
Sbjct: 304 SISLRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGI 363

Query: 397 LVLVHKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQ 456
           LVLV  CPVRELSLDHVYSFND+GLEALCSASYLE+LELVRCQEISD+GLQLVSQFPQLQ
Sbjct: 364 LVLVRMCPVRELSLDHVYSFNDMGLEALCSASYLESLELVRCQEISDDGLQLVSQFPQLQ 423

Query: 457 NLRLSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           NLRLSKCLGITDDGLK LVD Y KLESLVVEDCPQISERGVHGAAR ISFRQDLSWMY
Sbjct: 424 NLRLSKCLGITDDGLKLLVDTY-KLESLVVEDCPQISERGVHGAARAISFRQDLSWMY 480

BLAST of Lcy05g003350 vs. NCBI nr
Match: XP_022153907.1 (F-box/LRR-repeat protein 14 isoform X1 [Momordica charantia])

HSP 1 Score: 936.0 bits (2418), Expect = 1.4e-268
Identity = 467/514 (90.86%), Postives = 481/514 (93.58%), Query Frame = 0

Query: 1   MLSERPANNFGITDFELLYCFCSFCSPPYEILANGCYLVLQKCLVIMDSLPEVLIWEILN 60
           M SE    +FGI  F L   F  F      +L + C LVLQK LV MD+LPEVLIWEILN
Sbjct: 1   MWSESHGEDFGI--FRLFIQFLQF------LLIDYC-LVLQKRLVTMDALPEVLIWEILN 60

Query: 61  RVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLCLRFPNLGRIEITYS 120
           RVKKT+DRNSL+LSCKRLH LDKENR FLRVGCGLDPADEALTSLCLRFPNL RIEITYS
Sbjct: 61  RVKKTVDRNSLSLSCKRLHDLDKENRQFLRVGCGLDPADEALTSLCLRFPNLSRIEITYS 120

Query: 121 GWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTSCYKLSALKLNFTPR 180
           GWMSKLGKQLDDHGLFILS+HCPSLTDLTLSYCTFITDVGLRHLTSC  LSALKLNFTPR
Sbjct: 121 GWMSKLGKQLDDHGLFILSSHCPSLTDLTLSYCTFITDVGLRHLTSCSNLSALKLNFTPR 180

Query: 181 ITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK 240
           ITGCG+FSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK
Sbjct: 181 ITGCGVFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIK 240

Query: 241 LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLA 300
           LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPG+GLA
Sbjct: 241 LGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGKGLA 300

Query: 301 CVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD 360
           CVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD
Sbjct: 301 CVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD 360

Query: 361 ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVRELSLDHVYSFNDVG 420
           ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVH CPVREL+LDHVYSFND+G
Sbjct: 361 ESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHMCPVRELALDHVYSFNDMG 420

Query: 421 LEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGITDDGLKPLVDAYNK 480
           LEALCSASYLETLELVRCQEISDEGLQLVSQFPQL+ LRLSKCLGITDDGLKPLVDAY K
Sbjct: 421 LEALCSASYLETLELVRCQEISDEGLQLVSQFPQLRYLRLSKCLGITDDGLKPLVDAY-K 480

Query: 481 LESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           LESL VEDCPQISERGVHGAARTISFRQDLSWMY
Sbjct: 481 LESLAVEDCPQISERGVHGAARTISFRQDLSWMY 504

BLAST of Lcy05g003350 vs. NCBI nr
Match: KAG6593375.1 (F-box/LRR-repeat protein 14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 934.9 bits (2415), Expect = 3.0e-268
Identity = 455/474 (95.99%), Postives = 467/474 (98.52%), Query Frame = 0

Query: 41  QKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADE 100
           Q CLV+MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADE
Sbjct: 40  QNCLVVMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADE 99

Query: 101 ALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVG 160
           ALTSLCLRFPNL RIEITYSGWMSKLGKQLDD GLFILSNHCP LTDLTLSYCTFITDVG
Sbjct: 100 ALTSLCLRFPNLTRIEITYSGWMSKLGKQLDDRGLFILSNHCPCLTDLTLSYCTFITDVG 159

Query: 161 LRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLE 220
           LRHLTSCYKLS+LKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKL 
Sbjct: 160 LRHLTSCYKLSSLKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLG 219

Query: 221 TLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPC 280
           TLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWI C
Sbjct: 220 TLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWILC 279

Query: 281 DDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISL 340
           DDMLELSLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISL
Sbjct: 280 DDMLELSLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISL 339

Query: 341 RVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLV 400
           RVPSDFSLPLLA+NTLRLTDESLKALA+NC+HLESVRISFADGEFPSLSSFSLNGILVLV
Sbjct: 340 RVPSDFSLPLLAHNTLRLTDESLKALADNCAHLESVRISFADGEFPSLSSFSLNGILVLV 399

Query: 401 HKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRL 460
           HKCP++EL+LDHVYSFND+GLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRL
Sbjct: 400 HKCPIQELALDHVYSFNDMGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRL 459

Query: 461 SKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           SKCLGITDDGLKPLVDAY KLESLVVEDCPQISERGVHGAAR+ISFRQDLSWMY
Sbjct: 460 SKCLGITDDGLKPLVDAY-KLESLVVEDCPQISERGVHGAARSISFRQDLSWMY 512

BLAST of Lcy05g003350 vs. NCBI nr
Match: KAG7025724.1 (F-box/LRR-repeat protein 14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 453/475 (95.37%), Postives = 466/475 (98.11%), Query Frame = 0

Query: 40  LQKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPAD 99
           L  CLV+MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPAD
Sbjct: 43  LFNCLVVMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPAD 102

Query: 100 EALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDV 159
           EALTSLCLRFPNL RIEITYSGWMSKLGKQLDD GLFILSNHCP LTDLTLSYCTFITDV
Sbjct: 103 EALTSLCLRFPNLTRIEITYSGWMSKLGKQLDDRGLFILSNHCPCLTDLTLSYCTFITDV 162

Query: 160 GLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKL 219
           GLRHLTSCYKLS+LKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKL
Sbjct: 163 GLRHLTSCYKLSSLKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKL 222

Query: 220 ETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIP 279
             LEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWI 
Sbjct: 223 GILEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIL 282

Query: 280 CDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSIS 339
           CDDMLELSLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSIS
Sbjct: 283 CDDMLELSLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSIS 342

Query: 340 LRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVL 399
           LRVPSDFSLPLLA+NTLRLTDESLKALA+NC+HLESVRISFADGEFPSLSSFSLNGILVL
Sbjct: 343 LRVPSDFSLPLLAHNTLRLTDESLKALADNCAHLESVRISFADGEFPSLSSFSLNGILVL 402

Query: 400 VHKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLR 459
           +HKCP++EL+LDHVYSFND+GLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLR
Sbjct: 403 LHKCPIQELALDHVYSFNDMGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLR 462

Query: 460 LSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           LSKCLGITDDGLKPLVDAY KLESLVVEDCPQISERGVHGAAR+ISFRQDLSWMY
Sbjct: 463 LSKCLGITDDGLKPLVDAY-KLESLVVEDCPQISERGVHGAARSISFRQDLSWMY 516

BLAST of Lcy05g003350 vs. NCBI nr
Match: XP_038897691.1 (F-box/LRR-repeat protein 14 isoform X1 [Benincasa hispida])

HSP 1 Score: 927.2 bits (2395), Expect = 6.3e-266
Identity = 454/475 (95.58%), Postives = 464/475 (97.68%), Query Frame = 0

Query: 40  LQKCLVIMDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPAD 99
           + K LV MDSLPEVLIWEILNRVKKT DRNSLALSCKRLH LDKENRHFLRVGCGLDPAD
Sbjct: 1   MTKFLVTMDSLPEVLIWEILNRVKKTSDRNSLALSCKRLHSLDKENRHFLRVGCGLDPAD 60

Query: 100 EALTSLCLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDV 159
           EALTSLCLRFPNL RIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDV
Sbjct: 61  EALTSLCLRFPNLVRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDV 120

Query: 160 GLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKL 219
           GLR+LTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKL
Sbjct: 121 GLRNLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKL 180

Query: 220 ETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIP 279
           ETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWI 
Sbjct: 181 ETLEDLSIRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIS 240

Query: 280 CDDMLELSLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSIS 339
           CDDMLELSLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARES NLRSIS
Sbjct: 241 CDDMLELSLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESRNLRSIS 300

Query: 340 LRVPSDFSLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVL 399
           LRVPSDFSLPLLANN LRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVL
Sbjct: 301 LRVPSDFSLPLLANNPLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVL 360

Query: 400 VHKCPVRELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLR 459
           VH CPVREL+LDHVYSFND+GLEALCSASYLE+LELVRCQEISDEGLQLVSQFPQLQNLR
Sbjct: 361 VHMCPVRELALDHVYSFNDMGLEALCSASYLESLELVRCQEISDEGLQLVSQFPQLQNLR 420

Query: 460 LSKCLGITDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           LSKCLGITDDGLKPLVDAY KL+SL+VEDCPQISERG+HGAARTISFRQDLSWMY
Sbjct: 421 LSKCLGITDDGLKPLVDAY-KLKSLIVEDCPQISERGIHGAARTISFRQDLSWMY 474

BLAST of Lcy05g003350 vs. NCBI nr
Match: XP_023515079.1 (F-box/LRR-repeat protein 14 [Cucurbita pepo subsp. pepo] >XP_023515080.1 F-box/LRR-repeat protein 14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 925.6 bits (2391), Expect = 1.8e-265
Identity = 451/468 (96.37%), Postives = 462/468 (98.72%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC
Sbjct: 1   MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 60

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
           LRFPNL RIEITYSGWMSKLGKQLDD GLFILSNHCP LTDLTLSYCTFITDVGLRHLTS
Sbjct: 61  LRFPNLTRIEITYSGWMSKLGKQLDDRGLFILSNHCPCLTDLTLSYCTFITDVGLRHLTS 120

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           CYKLS+LKLNFTPRITGCGIFSIAVGCKNLT+LHLIRCLNVSSVEWLEYLGKL TLEDLS
Sbjct: 121 CYKLSSLKLNFTPRITGCGIFSIAVGCKNLTVLHLIRCLNVSSVEWLEYLGKLGTLEDLS 180

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWI CDDMLEL
Sbjct: 181 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWILCDDMLEL 240

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SLVNCIISPGRGLACVLGKCKNLQK+HLDMCVGVRDCDIISLARESHNLRSISLRVPSDF
Sbjct: 241 SLVNCIISPGRGLACVLGKCKNLQKVHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 300

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           SLPLLA+NTLRLTDESLKALA+NC+HLESVRISFADGEFPSLSSFSLNGILVLVHKCP++
Sbjct: 301 SLPLLAHNTLRLTDESLKALADNCAHLESVRISFADGEFPSLSSFSLNGILVLVHKCPIQ 360

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           EL+LDHVYSFND+GLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI
Sbjct: 361 ELALDHVYSFNDMGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 420

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDGLKPLVDAY KLESLVVEDCPQISERGVHGAAR+ISFRQDLSWMY
Sbjct: 421 TDDGLKPLVDAY-KLESLVVEDCPQISERGVHGAARSISFRQDLSWMY 467

BLAST of Lcy05g003350 vs. TAIR 10
Match: AT1G80570.2 (RNI-like superfamily protein )

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-204
Identity = 337/468 (72.01%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MD LP+ L+W+IL+++  T DRNSL+LSCKR   LD E R+ LR+GCGL PA +AL SLC
Sbjct: 14  MDELPDHLVWDILSKLHTTDDRNSLSLSCKRFFSLDNEQRYSLRIGCGLVPASDALLSLC 73

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
            RFPNL ++EI YSGWMSKLGKQ+DD GL +L+ +C SLTDLTLS+CTFITDVG+ HL+S
Sbjct: 74  RRFPNLSKVEIIYSGWMSKLGKQVDDQGLLVLTTNCHSLTDLTLSFCTFITDVGIGHLSS 133

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           C +LS+LKLNF PRITGCG+ S+AVGCK L  LHLIRCLNV+SVEWLEY GKLETLE+L 
Sbjct: 134 CPELSSLKLNFAPRITGCGVLSLAVGCKKLRRLHLIRCLNVASVEWLEYFGKLETLEELC 193

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           I+NCRAIGEGDLIKL +SWRKL  LQFEVDANYRYMKVYD+L V+RW KQ +PCD ++EL
Sbjct: 194 IKNCRAIGEGDLIKLRNSWRKLTSLQFEVDANYRYMKVYDQLDVERWPKQLVPCDSLVEL 253

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SL NCII+PGRGLACVL  CKNL+K+HLDMC GV D DII+L +++ +LRSISLRVPSDF
Sbjct: 254 SLGNCIIAPGRGLACVLRNCKNLEKLHLDMCTGVSDSDIIALVQKASHLRSISLRVPSDF 313

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           +LPLL N TLRLTDESL A+A++CS LES +ISF+DGEFPSL SF+L GI+ L+ KCPVR
Sbjct: 314 TLPLLNNITLRLTDESLSAIAQHCSKLESFKISFSDGEFPSLFSFTLQGIITLIQKCPVR 373

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           ELSLDHV  FND+G+EALCSA  LE LELV CQE+SDEGL LVSQFP L  L+LSKCLG+
Sbjct: 374 ELSLDHVCVFNDMGMEALCSAQKLEILELVHCQEVSDEGLILVSQFPSLNVLKLSKCLGV 433

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDG++PLV ++ KLE LVVEDCPQ+S RGVHGAA ++SF+QDLSWMY
Sbjct: 434 TDDGMRPLVGSH-KLELLVVEDCPQVSRRGVHGAATSVSFKQDLSWMY 480

BLAST of Lcy05g003350 vs. TAIR 10
Match: AT1G80570.3 (RNI-like superfamily protein )

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-204
Identity = 337/468 (72.01%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MD LP+ L+W+IL+++  T DRNSL+LSCKR   LD E R+ LR+GCGL PA +AL SLC
Sbjct: 1   MDELPDHLVWDILSKLHTTDDRNSLSLSCKRFFSLDNEQRYSLRIGCGLVPASDALLSLC 60

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
            RFPNL ++EI YSGWMSKLGKQ+DD GL +L+ +C SLTDLTLS+CTFITDVG+ HL+S
Sbjct: 61  RRFPNLSKVEIIYSGWMSKLGKQVDDQGLLVLTTNCHSLTDLTLSFCTFITDVGIGHLSS 120

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           C +LS+LKLNF PRITGCG+ S+AVGCK L  LHLIRCLNV+SVEWLEY GKLETLE+L 
Sbjct: 121 CPELSSLKLNFAPRITGCGVLSLAVGCKKLRRLHLIRCLNVASVEWLEYFGKLETLEELC 180

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           I+NCRAIGEGDLIKL +SWRKL  LQFEVDANYRYMKVYD+L V+RW KQ +PCD ++EL
Sbjct: 181 IKNCRAIGEGDLIKLRNSWRKLTSLQFEVDANYRYMKVYDQLDVERWPKQLVPCDSLVEL 240

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SL NCII+PGRGLACVL  CKNL+K+HLDMC GV D DII+L +++ +LRSISLRVPSDF
Sbjct: 241 SLGNCIIAPGRGLACVLRNCKNLEKLHLDMCTGVSDSDIIALVQKASHLRSISLRVPSDF 300

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           +LPLL N TLRLTDESL A+A++CS LES +ISF+DGEFPSL SF+L GI+ L+ KCPVR
Sbjct: 301 TLPLLNNITLRLTDESLSAIAQHCSKLESFKISFSDGEFPSLFSFTLQGIITLIQKCPVR 360

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           ELSLDHV  FND+G+EALCSA  LE LELV CQE+SDEGL LVSQFP L  L+LSKCLG+
Sbjct: 361 ELSLDHVCVFNDMGMEALCSAQKLEILELVHCQEVSDEGLILVSQFPSLNVLKLSKCLGV 420

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDG++PLV ++ KLE LVVEDCPQ+S RGVHGAA ++SF+QDLSWMY
Sbjct: 421 TDDGMRPLVGSH-KLELLVVEDCPQVSRRGVHGAATSVSFKQDLSWMY 467

BLAST of Lcy05g003350 vs. TAIR 10
Match: AT1G80570.1 (RNI-like superfamily protein )

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-204
Identity = 337/468 (72.01%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 47  MDSLPEVLIWEILNRVKKTIDRNSLALSCKRLHGLDKENRHFLRVGCGLDPADEALTSLC 106
           MD LP+ L+W+IL+++  T DRNSL+LSCKR   LD E R+ LR+GCGL PA +AL SLC
Sbjct: 1   MDELPDHLVWDILSKLHTTDDRNSLSLSCKRFFSLDNEQRYSLRIGCGLVPASDALLSLC 60

Query: 107 LRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS 166
            RFPNL ++EI YSGWMSKLGKQ+DD GL +L+ +C SLTDLTLS+CTFITDVG+ HL+S
Sbjct: 61  RRFPNLSKVEIIYSGWMSKLGKQVDDQGLLVLTTNCHSLTDLTLSFCTFITDVGIGHLSS 120

Query: 167 CYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHLIRCLNVSSVEWLEYLGKLETLEDLS 226
           C +LS+LKLNF PRITGCG+ S+AVGCK L  LHLIRCLNV+SVEWLEY GKLETLE+L 
Sbjct: 121 CPELSSLKLNFAPRITGCGVLSLAVGCKKLRRLHLIRCLNVASVEWLEYFGKLETLEELC 180

Query: 227 IRNCRAIGEGDLIKLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQWIPCDDMLEL 286
           I+NCRAIGEGDLIKL +SWRKL  LQFEVDANYRYMKVYD+L V+RW KQ +PCD ++EL
Sbjct: 181 IKNCRAIGEGDLIKLRNSWRKLTSLQFEVDANYRYMKVYDQLDVERWPKQLVPCDSLVEL 240

Query: 287 SLVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDF 346
           SL NCII+PGRGLACVL  CKNL+K+HLDMC GV D DII+L +++ +LRSISLRVPSDF
Sbjct: 241 SLGNCIIAPGRGLACVLRNCKNLEKLHLDMCTGVSDSDIIALVQKASHLRSISLRVPSDF 300

Query: 347 SLPLLANNTLRLTDESLKALAENCSHLESVRISFADGEFPSLSSFSLNGILVLVHKCPVR 406
           +LPLL N TLRLTDESL A+A++CS LES +ISF+DGEFPSL SF+L GI+ L+ KCPVR
Sbjct: 301 TLPLLNNITLRLTDESLSAIAQHCSKLESFKISFSDGEFPSLFSFTLQGIITLIQKCPVR 360

Query: 407 ELSLDHVYSFNDVGLEALCSASYLETLELVRCQEISDEGLQLVSQFPQLQNLRLSKCLGI 466
           ELSLDHV  FND+G+EALCSA  LE LELV CQE+SDEGL LVSQFP L  L+LSKCLG+
Sbjct: 361 ELSLDHVCVFNDMGMEALCSAQKLEILELVHCQEVSDEGLILVSQFPSLNVLKLSKCLGV 420

Query: 467 TDDGLKPLVDAYNKLESLVVEDCPQISERGVHGAARTISFRQDLSWMY 515
           TDDG++PLV ++ KLE LVVEDCPQ+S RGVHGAA ++SF+QDLSWMY
Sbjct: 421 TDDGMRPLVGSH-KLELLVVEDCPQVSRRGVHGAATSVSFKQDLSWMY 467

BLAST of Lcy05g003350 vs. TAIR 10
Match: AT5G01720.1 (RNI-like superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-26
Identity = 138/563 (24.51%), Postives = 224/563 (39.79%), Query Frame = 0

Query: 48  DSLPEVLIWEILNRVK-KTIDRNSLALSCKRLHGLDKENRHFL---------------RV 107
           D L E L++ IL+ +     D  S +L+CK  + L+ ++R  L               R 
Sbjct: 14  DLLSEELVFIILDLISPNPSDLKSFSLTCKSFYQLESKHRGSLKPLRSDYLPRILTRYRN 73

Query: 108 GCGLD------PADEALTSL-CLRFPNLGRIEITYSGWMSKLGKQLDDHGLFILSNHCPS 167
              LD        D AL+ + CL  P L  ++++ SG  S         GL  L+  C +
Sbjct: 74  TTDLDLTFCPRVTDYALSVVGCLSGPTLRSLDLSRSGSFSAA-------GLLRLALKCVN 133

Query: 168 LTDLTLSYCTFITDVGLRHLTSCYKLSALKLNFTPRITGCGIFSIAVGCKNLTILHL--- 227
           L ++ LS  T + D     +     L  LKL     +T  GI  IAVGCK L  + L   
Sbjct: 134 LVEIDLSNATEMRDADAAVVAEARSLERLKLGRCKMLTDMGIGCIAVGCKKLNTVSLKWC 193

Query: 228 ------------IRCLNVSSVEWLEYLG----------KLETLEDLSIRNCRAIGEGDLI 287
                       ++C ++ +++ L YL           KL+ LE+L +  C  + +  L 
Sbjct: 194 VGVGDLGVGLLAVKCKDIRTLD-LSYLPITGKCLHDILKLQHLEELLLEGCFGVDDDSLK 253

Query: 288 KLGHSWRKLKRLQFEVDANYRYMKVYDRLAVDRWQKQ--WIPCDDMLELS---------- 347
            L H  + LK+L      N  +  +   L+   + ++     C  ++ L           
Sbjct: 254 SLRHDCKSLKKLDASSCQNLTHRGLTSLLSGAGYLQRLDLSHCSSVISLDFASSLKKVSA 313

Query: 348 -----LVNCIISPGRGLACVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISL-- 407
                L  C ++P  GL  +   C +L+++ L  CV V D  + SL  +  +LR + +  
Sbjct: 314 LQSIRLDGCSVTPD-GLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDITC 373

Query: 408 -RVPSDFSLPLLANNTLRLTD-----------ESLKALAENCSHLESVRISFADGEFP-- 467
            R  S  S+  +AN+   L             E+   + + C  LE   +   D E    
Sbjct: 374 CRKLSRVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKCRLLE--ELDLTDNEIDDE 433

Query: 468 ---------SLSSFSLNGILVLVHK---------CPVRELSLDHVYSFNDVGLEALCSAS 511
                    SLSS  L   L +  K           +REL L       DVG+  +    
Sbjct: 434 GLKSISSCLSLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVGISTIAQGC 493

BLAST of Lcy05g003350 vs. TAIR 10
Match: AT5G27920.1 (F-box family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-24
Identity = 104/382 (27.23%), Postives = 180/382 (47.12%), Query Frame = 0

Query: 130 LDDHGLFILSNHCPSLTDLTLSYCTFITDVGLRHLTS-CYKLSALKLNFTPRITGCGIFS 189
           L D GL  +   C +L  ++L +C  I+D+G+  L   C  L +L +++  +IT   I S
Sbjct: 159 LSDVGLARIVVGCSNLNKISLKWCMEISDLGIDLLCKICKGLKSLDVSYL-KITNDSIRS 218

Query: 190 IAVGCKNLTILHLIRC--LNVSSVEWLEYLGKLETLEDLSIRNCRAIGEGDLIKL--GH- 249
           IA+  K L +L ++ C  ++   +++LE      +L+++ +  C  +    LI +  GH 
Sbjct: 219 IALLVK-LEVLDMVSCPLIDDGGLQFLE--NGSPSLQEVDVTRCDRVSLSGLISIVRGHP 278

Query: 250 --SWRKLKRLQFEVDANY-RYMKVYDRLAVDRWQKQWIPCDDMLELSLVNCIISPGRGLA 309
                K      EV  ++ +Y+K    L     +  WI    + + SLV+          
Sbjct: 279 DIQLLKASHCVSEVSGSFLKYIKGLKHL-----KTIWIDGAHVSDSSLVS---------- 338

Query: 310 CVLGKCKNLQKIHLDMCVGVRDCDIISLARESHNLRSISLRVPSDFSLPLLANNTLRLTD 369
            +   C++L +I L  CV V D  +ISLAR   NL++++L                 +TD
Sbjct: 339 -LSSSCRSLMEIGLSRCVDVTDIGMISLARNCLNLKTLNLACCG------------FVTD 398

Query: 370 ESLKALAENCSHLESVRIS----FADGEFPSLSSFSLNGILVLVHKCPVRELSLDHVYSF 429
            ++ A+A++C +L ++++       +    SL  +S+           V+EL L   Y  
Sbjct: 399 VAISAVAQSCRNLGTLKLESCHLITEKGLQSLGCYSM----------LVQELDLTDCYGV 458

Query: 430 NDVGLEALCSASYLETLELVRCQEISDEGL-QLVSQFPQLQNLRLSKCLGITDDGLKPLV 489
           ND GLE +   S L+ L+L  C  ISD+G+  + S+  +L  L L +C G  DDGL  L 
Sbjct: 459 NDRGLEYISKCSNLQRLKLGLCTNISDKGIFHIGSKCSKLLELDLYRCAGFGDDGLAALS 498

Query: 490 DAYNKLESLVVEDCPQISERGV 498
                L  L++  C ++++ GV
Sbjct: 519 RGCKSLNRLILSYCCELTDTGV 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EC972.1e-20372.01F-box/LRR-repeat protein 14 OS=Arabidopsis thaliana OX=3702 GN=FBL14 PE=2 SV=1[more]
Q8RWU51.6e-2524.51F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1[more]
Q9C5D24.9e-1924.93F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1[more]
A6H7794.3e-1524.73F-box/LRR-repeat protein 2 OS=Bos taurus OX=9913 GN=FBXL2 PE=2 SV=1[more]
Q58DG64.3e-1524.20F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DK866.6e-26990.86F-box/LRR-repeat protein 14 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1H5R51.5e-26595.94F-box/LRR-repeat protein 14 OS=Cucurbita moschata OX=3662 GN=LOC111460733 PE=4 S... [more]
A0A5A7UYS92.2e-26494.98F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1DM452.2e-26495.73F-box/LRR-repeat protein 14 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A1S3CEI12.2e-26494.98F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499827 P... [more]
Match NameE-valueIdentityDescription
XP_022153907.11.4e-26890.86F-box/LRR-repeat protein 14 isoform X1 [Momordica charantia][more]
KAG6593375.13.0e-26895.99F-box/LRR-repeat protein 14, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7025724.11.3e-26695.37F-box/LRR-repeat protein 14, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_038897691.16.3e-26695.58F-box/LRR-repeat protein 14 isoform X1 [Benincasa hispida][more]
XP_023515079.11.8e-26596.37F-box/LRR-repeat protein 14 [Cucurbita pepo subsp. pepo] >XP_023515080.1 F-box/L... [more]
Match NameE-valueIdentityDescription
AT1G80570.21.5e-20472.01RNI-like superfamily protein [more]
AT1G80570.31.5e-20472.01RNI-like superfamily protein [more]
AT1G80570.11.5e-20472.01RNI-like superfamily protein [more]
AT5G01720.11.1e-2624.51RNI-like superfamily protein [more]
AT5G27920.11.8e-2427.23F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 306..331
e-value: 130.0
score: 6.0
coord: 193..219
e-value: 120.0
score: 6.0
coord: 222..244
e-value: 440.0
score: 1.7
coord: 142..167
e-value: 6.0E-6
score: 35.8
coord: 427..452
e-value: 0.27
score: 20.3
coord: 453..477
e-value: 47.0
score: 9.3
coord: 478..503
e-value: 170.0
score: 5.0
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 428..447
e-value: 0.2
score: 11.9
coord: 141..164
e-value: 0.011
score: 15.7
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 270..510
e-value: 2.3E-30
score: 107.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 56..269
e-value: 1.7E-22
score: 81.4
NoneNo IPR availablePANTHERPTHR13318UNCHARACTERIZEDcoord: 48..507
NoneNo IPR availablePANTHERPTHR13318:SF170OS04G0371600 PROTEINcoord: 48..507
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 102..494

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g003350.1Lcy05g003350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding