Lcy05g001730 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g001730
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr05: 1644395 .. 1646864 (-)
RNA-Seq ExpressionLcy05g001730
SyntenyLcy05g001730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCTACCTTCTTCCCAAATCTTATGGATCTATCGGGTCTTTGTAGGGAAAATAACAATTTGGACGAATGGTGGGATGAAATCACAAGACAATTCAGTAGAGAAGAGTCAGAGAAGATTGTGATTATCCTATGGATAATTTGGAACTATATAAACAAAGTCACAATCGACAAGGCAAATGCAGATAATAACCTTCTTGTGCGGTGGATTAAAAGGAACTTTGAAGCGTAGAGAAAACGTACCTCCCATCATCAGATGGATTTTAGGCTGGAGAGCCTTCGGAAACATGAAAAGTGGACCCCCCTCCGAACTGCTTGAAATTAAACTTGGATGCCTCCTAGAATGAAGAATTATCGACATGGGGCGTGGGCTGGATAATCCGTGATTCTTCGGGATCTTTAGTTGAAGCTGGATGCAAACAAATCAAACGACGGTGGACCATTAAAACTCTGGAAATCTTAGCGATCAAGGAGGGTTTGAAGGCATACCTAGCGCAAAAGCGAAATCAGAGTGTCCATTTGATCGTGGAGGCGGATGTCCTCAAAGTTATCCGTGCGTTGAACTACGAAATCGCCGACATTTCCGAAAGCAAAATCGTGATGAACGAGGTGGAGGTTTTGGCGGCTGAAGCGAGCGTGATCTCTTTCATCAAATGCCCTAGAGTAGGGAATCAAATTGCACGAGCAGCGGCAGGTTTTTCGTCGGTGTTCTCTCTGTCATCGACATGTTTTTTGAATCGCCCCCATCTTCCATGCTGGAAGACATTTTTTTTTTAGGAAGATCATCTTCCTTTTTGGTTATTCTCTTTATTAGAAGAGGAGTTAGGTGTAACGAACGTTTTGCTGGCTTAATGAATTTATTCAGTTTTTTTTTTAAAAAAAAAAACACACACGCAAGCATGTCGCAGTGCTATTTTCTAGAAGGGATGATGATCACTCAAGACGTTTGTTTTACTAATGCAATAAAAAAAAAATCCTATCTTCTTACTCAATGTTTTAATCTGATTATATTCAAGCTTAAAAAAGTTTCATGACACAAAATTTTATGAAACATGTTCTATTATGACCACATCTTTCTCCACTTGTTAGCTCTCATTGATGTTGACATGCAACTAGCTAGCTAGCCCAATTATCACATTGACCAAATCTTTTGTAGCAACATCTTTCTAAAGATCAAGAATATTTGTCCATTCTTTAAAAAAAAAAAAAGGAATATTTGTCCATTTTAAGTTACCATTCACAAACCTAAGCTTTATTTAGTTACTTAAACAATAATTTTACACAAAAAAAAAAAGAAAAAAAAAAAACTACCTATTAAGATAATTATATTCATGATTGATTGACATATATTCTATTTTTGTCATAGGCTATTATTTTGCTATATTTACAATCAACCCACACTTTTTAAACACATATTCCCAAAATAAATCATTTGATATAGATTTCTTCAAAACCATTCTCATATCAAAAGAAGGAACACACACTTTTTGAGAAGAAATAAATCATTTATATTCCAAAAATATAGTTATCAATATTCCTTTTATGAGACTCTATGTAATGACACACAAAACACGATTTTGATAAAATTGGAATAATGTAAGAAACATCAAAATCAAGATCCCCACATCATATATTACTATTAATTTGACACAAACAAACATTTTTTTCACAATAGAAATTAAAAAAAAAAATAGTTCAGTATCTATCTATTTATTTCTCATTTTAACGTTGTTGTCTATCATCAATGTGACTATAATCTCCTCCATTCAAAATTTGAAAAACCAAAATCTTATAAGAATGTCACCACTGATTTTCAGCACTATCATTTTTCATTCCAAAATTAGACTACAGAGCTTTGACTTGAATAAGAACAATACCAATAAAATAAAATATTTTAATATTTGTCTCTAAAACATGTCTTATTTTAGTTCCAATACATTCAAATATTCAAATTTAACATATTTATTATTAATAAATCTTCAAATCAGTCAATCGTTTTATTTTTTTTTTCAAATTGGTATTAATATTTTATCAAATATTAATTTCAAAATTGTTTGTTTTTTTTTTTTTTTTTAAGAGTTTCAGATAATTGGAAAAAAAAAACCCAAATGAATACGGAATTTAAAATCCCAAAAAACTGGCAATACCCTATTCTTTTTAAGCTGTGTCTTTAGTTGAATCAGTACATTTTGGATTGCGATTTATTAGACTTGTTTTAGAAAGATCCCTTTCCTTTGTGAAATGGGTTCTCTTTTATTTTGGCAGTGAAAATACACAGAGCCCAGTTTTCATTGATGGAAATTTTCTCTGAAAACTCTGTTTCTGTTTCTGTTTATTGATTTTCACAGCCAAAAGAAGAAGCCAAAATCTGAGCTTTTTGGAGAGACAAACTGAAGCTACGAACCATAGCTCTGTTTCTCTAAACGGAATGCTATCTCCCATTGCCATCGCCATTGGCAGCCATTGTTGA

mRNA sequence

ATGAATGAAGAATTATCGACATGGGGCGTGGGCTGGATAATCCGTGATTCTTCGGGATCTTTAGTTGAAGCTGGATGCAAACAAATCAAACGACGGTGGACCATTAAAACTCTGGAAATCTTAGCGATCAAGGAGGGTTTGAAGGCATACCTAGCGCAAAAGCGAAATCAGAGTGTCCATTTGATCGTGGAGGCGGATGTCCTCAAAGTTATCCGTGCGTTGAACTACGAAATCGCCGACATTTCCGAAAGCAAAATCGTGATGAACGAGGTGGAGGTTTTGGCGGCTGAAGCGAGCGTGATCTCTTTCATCAAATGCCCTAGAGTAGGGAATCAAATTGCACGAGCAGCGGCAGCCAAAAGAAGAAGCCAAAATCTGAGCTTTTTGGAGAGACAAACTGAAGCTACGAACCATAGCTCTGTTTCTCTAAACGGAATGCTATCTCCCATTGCCATCGCCATTGGCAGCCATTGTTGA

Coding sequence (CDS)

ATGAATGAAGAATTATCGACATGGGGCGTGGGCTGGATAATCCGTGATTCTTCGGGATCTTTAGTTGAAGCTGGATGCAAACAAATCAAACGACGGTGGACCATTAAAACTCTGGAAATCTTAGCGATCAAGGAGGGTTTGAAGGCATACCTAGCGCAAAAGCGAAATCAGAGTGTCCATTTGATCGTGGAGGCGGATGTCCTCAAAGTTATCCGTGCGTTGAACTACGAAATCGCCGACATTTCCGAAAGCAAAATCGTGATGAACGAGGTGGAGGTTTTGGCGGCTGAAGCGAGCGTGATCTCTTTCATCAAATGCCCTAGAGTAGGGAATCAAATTGCACGAGCAGCGGCAGCCAAAAGAAGAAGCCAAAATCTGAGCTTTTTGGAGAGACAAACTGAAGCTACGAACCATAGCTCTGTTTCTCTAAACGGAATGCTATCTCCCATTGCCATCGCCATTGGCAGCCATTGTTGA

Protein sequence

MNEELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHLIVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARAAAAKRRSQNLSFLERQTEATNHSSVSLNGMLSPIAIAIGSHC
Homology
BLAST of Lcy05g001730 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 6.8e-07
Identity = 37/123 (30.08%), Postives = 69/123 (56.10%), Query Frame = 0

Query: 4   ELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHLIV 63
           + +T G+GWI+RD  G +++A C+ I+    I  LE++AI EGL+A + Q+  + +HL  
Sbjct: 98  DTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRA-IRQEHCRPIHL-- 157

Query: 64  EADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARAAAAKRRS 123
           E+D L+ I  L+ +  D +E   ++ E+  +  +  ++S     R  N++A   A +   
Sbjct: 158 ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRAME 217

Query: 124 QNL 127
            +L
Sbjct: 218 NDL 217

BLAST of Lcy05g001730 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 9.8e-06
Identity = 37/130 (28.46%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 4   ELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAY-------LAQKRN 63
           + +T G+GWI+RD  G +++A C+ I+    I  LE++AI EGL+A        + Q+  
Sbjct: 98  DTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHC 157

Query: 64  QSVHLIVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARA 123
           + +HL  E+D L+ I  L+ +  D +E   ++ E+  +  +  ++S     R  N++A  
Sbjct: 158 RPIHL--ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAHD 217

Query: 124 AAAKRRSQNL 127
            A +    +L
Sbjct: 218 LARRAMENDL 225

BLAST of Lcy05g001730 vs. ExPASy TrEMBL
Match: A0A5C7I858 (Ribonuclease H OS=Acer yangbiense OX=1000413 GN=EZV62_006523 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.3e-05
Identity = 35/109 (32.11%), Postives = 67/109 (61.47%), Query Frame = 0

Query: 1   MNEELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVH 60
           ++ +  + G G ++RDS G ++ A C+ ++  +  +  E LAI EGLK  +A   ++   
Sbjct: 442 LSSQSKSAGFGVVLRDSGGRVLAALCRNVRACYQPQVAEALAILEGLKFDVAGNYSK--- 501

Query: 61  LIVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRV 110
           +IVE+D L V++A+  ++A  S+  +V+N++ +L+A  S +SF   PR+
Sbjct: 502 VIVESDALVVVQAIINKVASSSDVGVVLNDIFLLSAHFSEVSFNFAPRL 547

BLAST of Lcy05g001730 vs. ExPASy TrEMBL
Match: A0A5C7IPE9 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_005913 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.8e-05
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 0

Query: 2   NEELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHL 61
           N  LST GVG  IRD  G +V A   Q+  ++T++  E++A++EGL+  LA   N  V L
Sbjct: 296 NNGLST-GVGAAIRDDKGLVVAARSNQLPGKFTVEMGELIALREGLQ--LAHFYNMKVDL 355

Query: 62  IVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIA 115
             E    KV+  LN  I  + ESK +MN++++L +   +   +   + GN +A
Sbjct: 356 -AEVISPKVVSILNDSIPLVGESKFIMNDIKMLVSVVGITKCLAVSKSGNSLA 404

BLAST of Lcy05g001730 vs. ExPASy TrEMBL
Match: A0A6P5S2T0 (uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.8e-04
Identity = 38/115 (33.04%), Postives = 63/115 (54.78%), Query Frame = 0

Query: 4   ELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHLIV 63
           +L   GVGW+IRDS G L+ AG +   R  +   +E+LAI+  L A       Q  H++V
Sbjct: 95  QLLRGGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALSAC---AHFQLTHVMV 154

Query: 64  EADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARAAA 119
           E+D  + I  LN +    S+ + ++ +++ L A  S +SF+  P+  N++A   A
Sbjct: 155 ESDTQRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVAHTVA 206

BLAST of Lcy05g001730 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-06
Identity = 37/123 (30.08%), Postives = 69/123 (56.10%), Query Frame = 0

Query: 4   ELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHLIV 63
           + +T G+GWI+RD  G +++A C+ I+    I  LE++AI EGL+A + Q+  + +HL  
Sbjct: 98  DTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRA-IRQEHCRPIHL-- 157

Query: 64  EADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARAAAAKRRS 123
           E+D L+ I  L+ +  D +E   ++ E+  +  +  ++S     R  N++A   A +   
Sbjct: 158 ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRAME 217

Query: 124 QNL 127
            +L
Sbjct: 218 NDL 217

BLAST of Lcy05g001730 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 63.2 bits (152), Expect = 2.4e-06
Identity = 39/108 (36.11%), Postives = 59/108 (54.63%), Query Frame = 0

Query: 2   NEELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHL 61
           +EE    GV W+I DS GS +   CK+++R+W +K LE  A+ EGLK YL  +   S + 
Sbjct: 15  SEEAGKGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTYLLIRETVS-YS 74

Query: 62  IVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRV 110
                 L++ R LN    D+ E   V++E+  L   A +ISF + P +
Sbjct: 75  HRPIIFLELARILNNTHMDLIELSNVVDEIFDLELLAGIISFFQVPSI 121

BLAST of Lcy05g001730 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-05
Identity = 37/130 (28.46%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 4   ELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAY-------LAQKRN 63
           + +T G+GWI+RD  G +++A C+ I+    I  LE++AI EGL+A        + Q+  
Sbjct: 98  DTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHC 157

Query: 64  QSVHLIVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIARA 123
           + +HL  E+D L+ I  L+ +  D +E   ++ E+  +  +  ++S     R  N++A  
Sbjct: 158 RPIHL--ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAHD 217

Query: 124 AAAKRRSQNL 127
            A +    +L
Sbjct: 218 LARRAMENDL 225

BLAST of Lcy05g001730 vs. NCBI nr
Match: TXG65248.1 (hypothetical protein EZV62_006523 [Acer yangbiense])

HSP 1 Score: 59.7 bits (143), Expect = 2.6e-05
Identity = 35/109 (32.11%), Postives = 67/109 (61.47%), Query Frame = 0

Query: 1   MNEELSTWGVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVH 60
           ++ +  + G G ++RDS G ++ A C+ ++  +  +  E LAI EGLK  +A   ++   
Sbjct: 442 LSSQSKSAGFGVVLRDSGGRVLAALCRNVRACYQPQVAEALAILEGLKFDVAGNYSK--- 501

Query: 61  LIVEADVLKVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRV 110
           +IVE+D L V++A+  ++A  S+  +V+N++ +L+A  S +SF   PR+
Sbjct: 502 VIVESDALVVVQAIINKVASSSDVGVVLNDIFLLSAHFSEVSFNFAPRL 547

BLAST of Lcy05g001730 vs. NCBI nr
Match: XP_021753633.1 (uncharacterized protein LOC110719026 [Chenopodium quinoa])

HSP 1 Score: 58.9 bits (141), Expect = 4.5e-05
Identity = 34/106 (32.08%), Postives = 61/106 (57.55%), Query Frame = 0

Query: 9   GVGWIIRDSSGSLVEAGCKQIKRRWTIKTLEILAIKEGLKAYLAQKRNQSVHLIVEADVL 68
           G+G ++RD  G +V  G +QIK  W ++  E  AI+ GL+            L+VE+D L
Sbjct: 178 GLGAVVRDEVGIVVAVGVQQIKASWEVRIAEAKAIEWGLRVAAGLGVRD---LVVESDCL 237

Query: 69  KVIRALNYEIADISESKIVMNEVEVLAAEASVISFIKCPRVGNQIA 115
           +VI+AL  + AD+SE  ++++++ +L +    + +    R GN++A
Sbjct: 238 QVIQALKNKTADLSELSLIIDDILLLCSSFDNVIWSYVKRGGNKVA 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CP266.8e-0730.08uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DSV19.8e-0628.46uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A5C7I8581.3e-0532.11Ribonuclease H OS=Acer yangbiense OX=1000413 GN=EZV62_006523 PE=4 SV=1[more]
A0A5C7IPE92.8e-0535.40RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_005913 ... [more]
A0A6P5S2T01.8e-0433.04uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE... [more]
Match NameE-valueIdentityDescription
XP_022143535.11.4e-0630.08uncharacterized protein LOC111013412 [Momordica charantia][more]
KAG6599977.12.4e-0636.11hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022156777.12.0e-0528.46uncharacterized protein LOC111023608 [Momordica charantia][more]
TXG65248.12.6e-0532.11hypothetical protein EZV62_006523 [Acer yangbiense][more]
XP_021753633.14.5e-0532.08uncharacterized protein LOC110719026 [Chenopodium quinoa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 9..119
e-value: 4.9E-13
score: 49.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..122
e-value: 8.5E-7
score: 31.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g001730.1Lcy05g001730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity