Lcy05g001130 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g001130
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
LocationChr05: 1094754 .. 1097820 (-)
RNA-Seq ExpressionLcy05g001130
SyntenyLcy05g001130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTTACACTCTCTTTCCTCTAAATACAACCAATCAAAATCAGATTTTGAGCTATAAATATATTGTTGAACCAAACCCAATAAGTCATCTTCTTCAAATCCTTCTCCAATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGGTGTTTTTTTTTTTTTCACTCTTTTTCTTCATCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTTGTGGGTTTTCTTATTTTTCTAAAAGTTTGCAACTTTTTTCTTTGACATGTGATTTGGCTATGGAATTCAATGGAAAAACTCAGATCCAAATGGTTAGTAGTTGATCTCTTCTTTGTTGTTCTTCATTTTTTAAAAAAATTTTACTTGATTTGTCAAATTTAAAATTACAAATTTGGAACTTTTGGATTATTGTCTCCATTATTGAAATTTCAAAACCAAAATTATAATTGAAATTTTAAAACCCATTATTTCATTGTTCATTTCCATTTTTGGGATATTTCAGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTCGTGTTAAATTACCACTAAACTCAAAAGCACTTGATCATATGTTTTTTTAATTATTCAAACCTCTGACTCTATGGTCGACGAAATAATTATAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGTAATTAATTAATTAATTAATTAAGTCAATTATTGAATTCAACTCTACTTTTTCAGCTTTTAGAAAAATATAAAAAGAAGGAAAAAAAAAAGTCTTCATCTGCCGACATGATGGAGTGATGGATTTGAATTCTATTTTGTTTTTATTTTTGCCTTTTAAACAAATCTATTTTAATTTCTAGATTTTTTCTATTACTAAACTTTTAAAAATTAGCTATTTTGATTCACACTATTATTTGGTTTCGTAAACGAAACACTAGGTTTTATCGATGGATCAATTTAGCACTTTTACATGCTCGTGTCAGTTCATTTTTTGCACATATTTGAAGTTATGTTATCGATTGTTAAAAAGATAAAGGATAAAAATCAATTAGTGATTTCTTTTTTTCTTGAAATACTGAGACTAAAATAGATATTTTTGGTAATTCAAGGATCAAAATGGAATAAGTTCAAAATATAAGTGGGTGGAAAGTTTTTCTCGTTAGACTCACATGTGGGGTTGATGAGAGAGATTACATTTACATTATGTAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGTGACTAAACATAAGAGACGGTGTTAAAGTCGTTTCATTTGGTTTTTGGTTTTTGATTTTTTTGTTTTTTAATTACAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTACGATAAATTATTTGAGAGGTTGGAACAATGTGGCGTAAAGTTTCACACATTTTGATGCATTTTTTTGTGGTGACTAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGGTGAGATTCTTTTTCATTCTATTATATATAAGGGTTTTTAAGAAAGGAATTTTGTTCTCCACTATGATTTTTGAGCTGAATTTATGGAATCAATTGGTTCAAAACACTCAACTAGGTTTGCTTTTAGGACAAAGAAGTTGTTCAAAAAGTGCACTTATCCATGTATTCAAACACCATATTCTAATCAATTAAAAAAACTCCATAAAGTTGTGTGACACATTCAATCCCACTTAGACTTTTGGTAGGACTTACAAAGTGAGTCTTGATATTCATATTTATTGAATAAAAAATCGA

mRNA sequence

TATTTTACACTCTCTTTCCTCTAAATACAACCAATCAAAATCAGATTTTGAGCTATAAATATATTGTTGAACCAAACCCAATAAGTCATCTTCTTCAAATCCTTCTCCAATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGATCCAAATGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGGTGAGATTCTTTTTCATTCTATTATATATAAGGGTTTTTAAGAAAGGAATTTTGTTCTCCACTATGATTTTTGAGCTGAATTTATGGAATCAATTGGTTCAAAACACTCAACTAGGTTTGCTTTTAGGACAAAGAAGTTGTTCAAAAAGTGCACTTATCCATGTATTCAAACACCATATTCTAATCAATTAAAAAAACTCCATAAAGTTGTGTGACACATTCAATCCCACTTAGACTTTTGGTAGGACTTACAAAGTGAGTCTTGATATTCATATTTATTGAATAAAAAATCGA

Coding sequence (CDS)

ATGGCTTCCTTGTCTCTCTGGTTCATCTCTTTCCTTGGTCTCTTGCTTGGCCATGGAGTTTTGCAGCTTCAAGCTTCCCACCATGTTTACAAAGCTCAAACTTCTCACTTTGTTCCTCATGATTATCAACAACCCTATAGAACTTCTTACCATTTTCAACCTCCAAAGAATTGGATGAATGATCCAAATGGTCCAATGATATTCAAGGGAATTTATCACCTGTTTTACCAATACAACCCAAAAGGAGCAGTGTGGGGCAACATTGTGTGGGCCCACTCCACATCAACGGACCTGATCAACTGGGTACCCCACGAGTACGCCATCTATCCATCTCAGCCGTCGGATATCAACGGCTGCTGGTCGGGCTCCGCCACCATCCTCCCCGGCGCCAAACCGGCGATTCTCTACACAGGAATTAACCCTAAAAGCCAACAAGTTCAAAACTTGGCTGTCCCCAAGAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTAATGGCCCCCACCCCTGAGAATCACATCAACTCCAGCTCCTTCCGGGACCCCACCACCGCCTGGCTCGGCCCCGATCGGCAATGGCGAGTCATCATCGGGAGCAAAGTTGACGCCCGTGGACTCGCCATCATGTACCGAAGCAAGGATTTCGTCCAGTGGGATCAGGTTGACCACCCGCTTCATTATGCCGATGGCATGGGAATGTGGGAGTGTCCGGATTTCTTTCCGGTGGTGAAGAAAGGTCGGACCGGCATCGACACGGTGGTGAACGGACCGCACGTGAAGCACGTGTTGAAGATAAGCTTGGATGATACTAAGCATGACCTTTATACTATTGGAACATATAAGTTGGATAAGGATATCTATGTTCCAGATAAAGGATCCATAGAGAATTACAATGGATTGAGGTATGATTATGGGAAATATTATGCTTCAAAAACTTTTTTTGACAGCCAAAAGAAAAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCTTCAAGTGTTGATGATGATATCAAGAAAGGATGGTCTGGAGTCCAGGCAATTCCAAGGACGGTTTGGTTGGATGCATCTGGAAAGCAATTGATTCAATGGCCGATCGTGGAAATTCATAAGCTTCGAAAGGACAAAGTTAAGTTGACGAACAAAGTGTTGAAGAAAGGATCAGTGGTTGAAGTAAAAGGAGTAACAGCAGTGCAGGCAGATGTTGAGGTTTCATTTAAAGTAAAAAACTTCAACAAAGCTGAAGTGTTGAAACCTGAATGGAAAGACCCACAATTGCTTTGTAGCCAAAAGGGAACAGCTGCAGATGGTGGAGTAGGCCCATTTGGGCTTCTAGTTTTGGCTTCAAAGGACTTGAAAGAGTACACTTCAGTGTTGTTTAGGATATTCAAACACTACAACAAATACGTCGTGCTTTTGTGTAGTGACCAAAGCAGGTCTTCGTTGAACTCAAATAATGACAAGACAACGTACGGAGCATTCTTAGATGTCGATCCAGTTAGTGAAAATCTCTCGCTAAGAAGCTTGGTGAGATTCTTTTTCATTCTATTATATATAAGGGTTTTTAAGAAAGGAATTTTGTTCTCCACTATGATTTTTGAGCTGAATTTATGGAATCAATTGGTTCAAAACACTCAACTAGGTTTGCTTTTAGGACAAAGAAGTTGTTCAAAAAGTGCACTTATCCATGTATTCAAACACCATATTCTAATCAATTAA

Protein sequence

MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMNDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLVRFFFILLYIRVFKKGILFSTMIFELNLWNQLVQNTQLGLLLGQRSCSKSALIHVFKHHILIN
Homology
BLAST of Lcy05g001130 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.8e-211
Identity = 355/523 (67.88%), Postives = 420/523 (80.31%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
           NDPNGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
           WSGSATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
           IN+SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
           WECPDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
            G   +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
           +WLD SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
             KA+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           KYVVL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+L+
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 526

BLAST of Lcy05g001130 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.2e-201
Identity = 333/521 (63.92%), Postives = 405/521 (77.74%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVY---KAQTSHFVPHDYQQPYRTSYHFQPPKN 60
           MA  S++ +S   L+    V+ ++A+HHVY   +  +SH       QPYRT+YHFQP KN
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKN 60

Query: 61  WMNDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDIN 120
           W+NDPNGPM + G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P ++AI+PSQPSDI 
Sbjct: 61  WINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIK 120

Query: 121 GCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPE 180
           GCWSGSATILPG KPAILYTGI+P + QVQN+A+PKN+SDP LREW KSPKNPLM PT  
Sbjct: 121 GCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIA 180

Query: 181 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 240
           N INSSSFRDPTT+WLG D  WRV+IGSK+D +G+AI+Y+SK+FV W +  HPLH A+G 
Sbjct: 181 NKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGT 240

Query: 241 GMWECPDFFPVVKKG--RTGIDTVVNG-PHVKHVLKISLDDTKHDLYTIGTYKLDKDIYV 300
           GMWECPDF+PV+ K   RTG+DT  NG   V+HVLK+SLDDTKHD Y IG+Y + KD++V
Sbjct: 241 GMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFV 300

Query: 301 PDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 360
           P+ G  +N   LRYDYGKYYASKTFFD  K RR+L GWVNESSSV DD+KKGWSG+  IP
Sbjct: 301 PENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIP 360

Query: 361 RTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVK 420
           R +WL  SGKQL+QWP+ EI  LR + V    KV+K G  + + GV +VQADVE+SF++ 
Sbjct: 361 REIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEIS 420

Query: 421 NFNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKY 480
           +  K E L+ +W DPQLLCSQKG    GGVGPFGLLV AS+ LKEYT+V FRIFK+ +K 
Sbjct: 421 DLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQDKN 480

Query: 481 VVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           +VL+CSDQSRSSLN +ND T+YG F+DVDP+ E LSLR+L+
Sbjct: 481 LVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLI 517

BLAST of Lcy05g001130 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 652.5 bits (1682), Expect = 4.4e-186
Identity = 315/513 (61.40%), Postives = 373/513 (72.71%), Query Frame = 0

Query: 12  LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMNDPNGPMIFKGI 71
           L + L + +  L+AS H            D  QPYRT YHFQP KNWMNDPNGPMI+KGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
           AWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV 
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
             G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YVPD G ++N +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
           DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
           WPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK   KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
           PQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +      KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           SRSSLN  NDK+T+GAF+ +DP  + +SLR+L+
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLI 516

BLAST of Lcy05g001130 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 8.3e-185
Identity = 316/518 (61.00%), Postives = 390/518 (75.29%), Query Frame = 0

Query: 9   ISFLGLLLGHGVLQL---QASHHVYKAQTSHFVPHDYQ-----QPYRTSYHFQPPKNWMN 68
           +S   +LL HG++ +   QA HH +       + +D+Q      PYRT+YHFQ PKNWMN
Sbjct: 11  LSISMMLLSHGMIIIAKDQAIHH-HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMN 70

Query: 69  DPNGPMIFKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 128
           DPNGPMI+KGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGC
Sbjct: 71  DPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGC 130

Query: 129 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMA--PTPE 188
           WSGS TILP  KP ILYTGIN K+ QVQNLA+PKNLSDPYL+EW+K P+NPLMA  PT  
Sbjct: 131 WSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNN 190

Query: 189 NHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGM 248
           N+IN+SSFRDP+TAW   D +WRVI+G++   RGLA+++ S DFV+W+   +PLH  +G 
Sbjct: 191 NNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGN 250

Query: 249 GMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDK 308
           G+WECPDFFPV      G DT + G  VKHVLK+SL DT+++ YTIG Y ++KDIYVPD+
Sbjct: 251 GIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDE 310

Query: 309 GSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTV 368
           GSIE+  GLRYDYGK+YASK+FFD +  RR+LWGWVNESS   DDIKKGWSGVQAIPRTV
Sbjct: 311 GSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTV 370

Query: 369 WLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFN 428
            LD SGKQL+QWP+ E+  LR++ V+L ++V+K GS+VE+  +TA QADVE+SFK+   N
Sbjct: 371 VLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESN 430

Query: 429 KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVL 488
             E L     +PQ+LCSQKG +  G  GPFGLL LAS  L+EYT+V FRIFK  NKYVVL
Sbjct: 431 YVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVL 490

Query: 489 LCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           +CSDQ+RSSLN   DK ++G F+DVDP++E+LSLR L+
Sbjct: 491 MCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILI 527

BLAST of Lcy05g001130 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 644.0 bits (1660), Expect = 1.6e-183
Identity = 311/487 (63.86%), Postives = 378/487 (77.62%), Query Frame = 0

Query: 38  VPHDYQ-QPYRTSYHFQPPKNWMNDPNGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHST 97
           VP++ + QPYRT YHFQPPKNWMNDPNGPMI+KGIYHLFYQ+N  GAV      VW H+T
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHAT 92

Query: 98  STDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKN 157
           STDLINW+    AI PS+PSDINGCWSGS TILP  KP ILYTG +  ++QVQNL  PKN
Sbjct: 93  STDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKN 152

Query: 158 LSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAI 217
           L+DPYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI
Sbjct: 153 LTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAI 212

Query: 218 MYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISL 277
           ++ SKDFV W Q   PLHY DG GMWECPDFFPV +    G+DT   +GP VKHVLK+SL
Sbjct: 213 LHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSL 272

Query: 278 DDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWV 337
            DT +D YTIGTY   +D+YVPDKG +++    RYDYGK+YASKTF+DS  +RR+LWGWV
Sbjct: 273 TDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWV 332

Query: 338 NESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGS 397
           NESS   D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR  +VK  NK+LK GS
Sbjct: 333 NESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGS 392

Query: 398 VVEVKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLV 457
           V+EV GVTA QADVEV FKV  F+  KA+V++P W DPQL+CSQ+   A  G+GPFGL+V
Sbjct: 393 VMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMV 452

Query: 458 LASKDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSEN 516
           LASK+++EYTSV  RIF+   +  ++VV++CSDQS SSL   NDKTTYGAFLD+ P  + 
Sbjct: 453 LASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QP 512

BLAST of Lcy05g001130 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.6e-269
Identity = 441/515 (85.63%), Postives = 471/515 (91.46%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSL+
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLI 515

BLAST of Lcy05g001130 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.4e-267
Identity = 432/515 (83.88%), Postives = 474/515 (92.04%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SLWF SFL + L HGV +L+ASHHVY+AQTS  VP D+QQPYRTSYHFQPPKNWMN
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPH++AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPA+LYTGINP++ QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTP+N+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DRQWRV+IGSK++ARGLAIMYRSKDF+QWDQV++PLHYAD  GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT VNGP VKHVLK+SLDDTKHD YT+GTY + KD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQL+QWPIVEI KLRKDKVKL NKVLKKGSV+EVKGVTA Q DVEVSFKVKNF KA+
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           VLKPEWKDPQLLC+QKG A + GVGPFGLL+LASKDLKEYTSV F++FK   KYVVL+CS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLNSNNDKTTYGAF+D+DPV ENLSLRSL+
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLI 515

BLAST of Lcy05g001130 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.1e-267
Identity = 437/515 (84.85%), Postives = 473/515 (91.84%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H ++QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSKVD RGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           ++Y+GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KLTNKVL+KGS++ VKGVTA QADV+VSFKVK+FNKA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           +LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN++NDKT YGAFLDVDPV +N+SLRSL+
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLI 515

BLAST of Lcy05g001130 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 5.5e-264
Identity = 434/515 (84.27%), Postives = 464/515 (90.10%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FIS L  LL HG L+LQASHHVY AQ S  + H Y+QPYRTSYHFQPPKNW+N
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLA+PKNLSDPYLREWVKSP NPLMAPTP+N I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D QWRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR GIDT VNG HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDK S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDD+KKGWSGVQAIPR VWLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KL NKV+KKGS++ VKGVTA QADVEVSFKVK+F KA+
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL CS+ G AA GGVGPFGLLVLASKDL+EYTSV FR+FK  +KYVVL+CS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSL+
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLI 517

BLAST of Lcy05g001130 vs. ExPASy TrEMBL
Match: A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 2.0e-258
Identity = 428/523 (81.84%), Postives = 469/523 (89.67%), Query Frame = 0

Query: 3   SLSLWFIS----FLGLLLGHGVLQLQASHHVYKA--QTSHFVPHDY-QQPYRTSYHFQPP 62
           S SL F++     + LL GH  LQLQA HHVY+A  +TSHFV H + QQPYRTSYHFQPP
Sbjct: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64

Query: 63  KNWMNDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSD 122
           KNW+NDPNGPMI+KGIYH FYQYNPKGAVWGNIVWAHSTSTDLINW PH++AI+PSQPSD
Sbjct: 65  KNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124

Query: 123 INGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182
           INGCWSGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184

Query: 183 PENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYAD 242
           P+NHIN SSFRDPTTAWLG D +WRVIIGSK+DARGLAIMYRSKDFV+W+QVDHPL Y D
Sbjct: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244

Query: 243 GMGMWECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVP 302
            MGMWECPDFFPV K GRTG++  V+G HVKH+LK+SLDDTKHD+YT+GTY   KDIY+P
Sbjct: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304

Query: 303 DKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 362
           +KGSIE YNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR
Sbjct: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364

Query: 363 TVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 422
            +WLDASGKQLIQWPIVEI KLR +KVKLTN+VLKKGS +EVKGVTA QADVE+SFKV+N
Sbjct: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424

Query: 423 FNKAEVLKPEWKDPQLLCSQKGTAA---DGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN 482
             KAE+LKPEWKDPQLLCSQKGT++    G VGPFGLLVLAS DLKEYTSV F +FK  +
Sbjct: 425 LKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKPH 484

Query: 483 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           KYVVL+CSDQSRSSL+SNNDKTTYGAFLDVDP  EN+SLRSL+
Sbjct: 485 KYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLI 527

BLAST of Lcy05g001130 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 948.0 bits (2449), Expect = 3.9e-272
Identity = 443/515 (86.02%), Postives = 477/515 (92.62%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  L GHGVLQLQASHHVY+AQ S+ +PH YQQPYRTSYHFQPPKNW+N
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSK++ARGLAIMY+SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+G Y L+KDIYVP+KGSI
Sbjct: 241 ECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPRT+WLD
Sbjct: 301 EGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDKVKLTNKVLKKGS++EVKGVTA QADVEVSFKVKNF KA+
Sbjct: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           +LKPEWKDPQLLCSQKG    GGVG FGLLVLASKDLKEYTS+ F IFK   KY+VL+CS
Sbjct: 421 ILKPEWKDPQLLCSQKGATTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN NNDKTTYGAFL+VDPVSE+LSLRSL+
Sbjct: 481 DQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLI 515

BLAST of Lcy05g001130 vs. NCBI nr
Match: XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939.1 bits (2426), Expect = 1.8e-269
Identity = 442/515 (85.83%), Postives = 473/515 (91.84%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY AQ S  + H ++QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K G  G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGHAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E+Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KLTNKVLKKGS+V VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 RLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSL+
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLI 515

BLAST of Lcy05g001130 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 935.6 bits (2417), Expect = 2.0e-268
Identity = 441/515 (85.63%), Postives = 471/515 (91.46%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSK+DARGLAIM++SKDFV+WDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR VWLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPIVEI KLRKDKVKLTNKVLKKGS++ VKGVTA QADV+VSFKVK+FNKA+
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
            LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN+NNDKTTYGAFLDVDPV EN+SLRSL+
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLI 515

BLAST of Lcy05g001130 vs. NCBI nr
Match: XP_022153896.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Momordica charantia])

HSP 1 Score: 931.8 bits (2407), Expect = 2.9e-267
Identity = 432/515 (83.88%), Postives = 474/515 (92.04%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SLWF SFL + L HGV +L+ASHHVY+AQTS  VP D+QQPYRTSYHFQPPKNWMN
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPH++AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPA+LYTGINP++ QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTP+N+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLG DRQWRV+IGSK++ARGLAIMYRSKDF+QWDQV++PLHYAD  GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K  R G+DT VNGP VKHVLK+SLDDTKHD YT+GTY + KD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           E Y GLRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQL+QWPIVEI KLRKDKVKL NKVLKKGSV+EVKGVTA Q DVEVSFKVKNF KA+
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           VLKPEWKDPQLLC+QKG A + GVGPFGLL+LASKDLKEYTSV F++FK   KYVVL+CS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLNSNNDKTTYGAF+D+DPV ENLSLRSL+
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLI 515

BLAST of Lcy05g001130 vs. NCBI nr
Match: XP_022954013.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 930.6 bits (2404), Expect = 6.4e-267
Identity = 437/515 (84.85%), Postives = 473/515 (91.84%), Query Frame = 0

Query: 1   MASLSLWFISFLGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFL  LL HG L+LQASHHVY  Q S  + H ++QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  DPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCW 120
           DPNGPMI+KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+YAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHI 180
           SGSATILPG KPAILYTGINPK+QQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP+NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSKVD RGLAIM+RSKDFV+WDQVDHPLHYADGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVVKKGRTGIDTVVNGPHVKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSI 300
           ECPDFFPV K GR G+DT V+G HVKHVLK+SLDDTKHD YT+GTY L KDIYVPDKGSI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 ENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360
           ++Y+GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAE 420
           ASGKQLIQWPI EI KLRKDK+KLTNKVL+KGS++ VKGVTA QADV+VSFKVK+FNKA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 VLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYNKYVVLLCS 480
           +LK EWKDPQL+CSQ G AA GGVGPFGLLVLAS +L+EYTSV FRIFK  +KYVVL+CS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           DQSRSSLN++NDKT YGAFLDVDPV +N+SLRSL+
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLI 515

BLAST of Lcy05g001130 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 734.6 bits (1895), Expect = 6.3e-212
Identity = 355/523 (67.88%), Postives = 420/523 (80.31%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
           NDPNGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
           WSGSATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
           IN+SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
           WECPDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
            G   +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
           +WLD SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
             KA+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           KYVVL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+L+
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 526

BLAST of Lcy05g001130 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 721.8 bits (1862), Expect = 4.2e-208
Identity = 352/523 (67.30%), Postives = 417/523 (79.73%), Query Frame = 0

Query: 2   ASLSLWFISFLGLLLGHGVLQLQASHHVYK--AQTSHFVPHDYQQPYRTSYHFQPPKNWM 61
           +++ LW +  L LL+G+ V+ L+ASHHVYK   Q+++       QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIFKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEYAIYPSQPSDINGC 121
              NGPMI+KGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  ---NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENH 181
           WSGSATILP  KP ILYTGI+PK+QQVQN+A PKNLSDPYLREW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAIMYRSKDFVQWDQVDHPLHYADGMGM 241
           IN+SSFRDPTTAWLG D++WRVIIGSK+  RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVVKKGRTGIDTVVNG-PH--VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPD 301
           WECPDFFPV + G  G++T   G P+  +KHVLKISLDDTKHD YTIGTY   KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRT 361
            G   +    RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 VWLDASGKQLIQWPIVEIHKLRKDKVK-LTNKVLKKGSVVEVKGVTAVQADVEVSFKVKN 421
           +WLD SGKQLIQWP+ E+ +LR  +VK L NKVLK GS +EV GVTA QADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FNKAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFK---HYN 481
             KA+V++P W DPQL+CS+   +   G+GPFGL+VLASK+L+EYTSV FRIFK   + N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           KYVVL+CSDQSRSSL  +NDKTTYGAF+D++P  + LSLR+L+
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 523

BLAST of Lcy05g001130 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 652.5 bits (1682), Expect = 3.1e-187
Identity = 315/513 (61.40%), Postives = 373/513 (72.71%), Query Frame = 0

Query: 12  LGLLLGHGVLQLQASHHVYKAQTSHFVPHDYQQPYRTSYHFQPPKNWMNDPNGPMIFKGI 71
           L + L + +  L+AS H            D  QPYRT YHFQP KNWMNDPNGPMI+KGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWSGSATILPGA 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVV 251
           AWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV 
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRY 311
             G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YVPD G ++N +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQ 371
           DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ PR +WLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKAEVLKPEWKD 431
           WPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK   KA+V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------KYVVLLCSDQ 491
           PQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +      KYVVL+CSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           SRSSLN  NDK+T+GAF+ +DP  + +SLR+L+
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLI 516

BLAST of Lcy05g001130 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 631.3 bits (1627), Expect = 7.5e-181
Identity = 308/487 (63.24%), Postives = 375/487 (77.00%), Query Frame = 0

Query: 38  VPHDYQ-QPYRTSYHFQPPKNWMNDPNGPMIFKGIYHLFYQYNPKGAVW--GNIVWAHST 97
           VP++ + QPYRT YHFQPPKNWM   NGPMI+KGIYHLFYQ+N  GAV      VW H+T
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWM---NGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHAT 92

Query: 98  STDLINWVPHEYAIYPSQPSDINGCWSGSATILPGAKPAILYTGINPKSQQVQNLAVPKN 157
           STDLINW+    AI PS+PSDINGCWSGS TILP  KP ILYTG +  ++QVQNL  PKN
Sbjct: 93  STDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKN 152

Query: 158 LSDPYLREWVKSPKNPLMAPTPENHINSSSFRDPTTAWLGPDRQWRVIIGSKVDARGLAI 217
           L+DPYLR W KSP+NPL+ P+P NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI
Sbjct: 153 LTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAI 212

Query: 218 MYRSKDFVQWDQVDHPLHYADGMGMWECPDFFPVVKKGRTGIDT-VVNGPHVKHVLKISL 277
           ++ SKDFV W Q   PLHY DG GMWECPDFFPV +    G+DT   +GP VKHVLK+SL
Sbjct: 213 LHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSL 272

Query: 278 DDTKHDLYTIGTYKLDKDIYVPDKGSIENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWV 337
            DT +D YTIGTY   +D+YVPDKG +++    RYDYGK+YASKTF+DS  +RR+LWGWV
Sbjct: 273 TDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWV 332

Query: 338 NESSSVDDDIKKGWSGVQAIPRTVWLDASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGS 397
           NESS   D+IKKGW+G+QAIPR VWLD SGK+L+QWP+ EI +LR  +VK  NK+LK GS
Sbjct: 333 NESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGS 392

Query: 398 VVEVKGVTAVQADVEVSFKVKNFN--KAEVLKPEWKDPQLLCSQKGTAADGGVGPFGLLV 457
           V+EV GVTA QADVEV FKV  F+  KA+V++P W DPQL+CSQ+   A  G+GPFGL+V
Sbjct: 393 VMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRN--ASSGLGPFGLMV 452

Query: 458 LASKDLKEYTSVLFRIFK---HYNKYVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSEN 516
           LASK+++EYTSV  RIF+   +  ++VV++CSDQS SSL   NDKTTYGAFLD+ P  + 
Sbjct: 453 LASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QP 512

BLAST of Lcy05g001130 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 608.6 bits (1568), Expect = 5.2e-174
Identity = 290/462 (62.77%), Postives = 345/462 (74.68%), Query Frame = 0

Query: 63  NGPMIFKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEYAIYPSQPSDINGCWS 122
           +GPMI+KGIYHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWS
Sbjct: 40  SGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWS 99

Query: 123 GSATILPGAKPAILYTGINPKSQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPENHIN 182
           GS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN
Sbjct: 100 GSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGIN 159

Query: 183 SSSFRDPTTAWLGPDRQWRVIIGSKV-DARGLAIMYRSKDFVQWDQVDHPLHYADGMGMW 242
              FRDPTTAWLG D +WRVI+GS   D RGLAI+Y+S+DF  W Q   PLHY D  GMW
Sbjct: 160 PDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMW 219

Query: 243 ECPDFFPVVKKGRTGIDTVVNGPH-VKHVLKISLDDTKHDLYTIGTYKLDKDIYVPDKGS 302
           ECPDFFPV   G  G++T   G + +KHVLK+SL +T HD YTIG+Y  +KD+YVPD G 
Sbjct: 220 ECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGF 279

Query: 303 IENYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWL 362
           ++N +  R DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ PR +WL
Sbjct: 280 VQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWL 339

Query: 363 DASGKQLIQWPIVEIHKLRKDKVKLTNKVLKKGSVVEVKGVTAVQADVEVSFKVKNFNKA 422
           D SGK+L+QWPI EI  LR  +V    KVLK GS ++V GVTA QADVEVSFKVK   KA
Sbjct: 340 DESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKA 399

Query: 423 EVLKPEWKDPQLLCSQKGTAADGGVGPFGLLVLASKDLKEYTSVLFRIFKHYN------K 482
           +V++P W DPQ +CSQ   +   G+GPFGL+VLAS D++EYTSV FRIFK  +      K
Sbjct: 400 DVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTK 459

Query: 483 YVVLLCSDQSRSSLNSNNDKTTYGAFLDVDPVSENLSLRSLV 516
           YVVL+CSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR+L+
Sbjct: 460 YVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLI 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438668.8e-21167.88Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q430892.2e-20163.92Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q67XZ34.4e-18661.40Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q70XE68.3e-18561.00Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q9LIB91.6e-18363.86Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GPU79.6e-26985.63beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1DM231.4e-26783.88beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia ... [more]
A0A6J1GPY13.1e-26784.85beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1JP625.5e-26484.27beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... [more]
A0A2I4JRK22.0e-25881.84Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038898203.13.9e-27286.02beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida][more]
XP_023547447.11.8e-26985.83beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... [more]
XP_022954011.12.0e-26885.63beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... [more]
XP_022153896.12.9e-26783.88beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Momordica charantia][more]
XP_022954013.16.4e-26784.85beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G13790.16.3e-21267.88Glycosyl hydrolases family 32 protein [more]
AT3G13790.24.2e-20867.30Glycosyl hydrolases family 32 protein [more]
AT1G55120.13.1e-18761.40beta-fructofuranosidase 5 [more]
AT3G13784.17.5e-18163.24cell wall invertase 5 [more]
AT1G55120.35.2e-17462.77beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 51..522
e-value: 9.1E-231
score: 782.6
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 55..381
e-value: 9.8E-118
score: 395.3
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 42..371
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 373..495
e-value: 3.2E-9
score: 37.0
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 51..370
e-value: 3.7E-108
score: 361.7
NoneNo IPR availableGENE3D2.60.120.560coord: 382..517
e-value: 1.0E-50
score: 174.0
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 7..515
NoneNo IPR availablePANTHERPTHR31953:SF51CELL-WALL INVERTASEcoord: 7..515
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 57..359
e-value: 3.41741E-168
score: 477.649
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 51..64
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 370..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g001130.1Lcy05g001130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds