Lcy05g000300 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g000300
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	(R)-mandelonitrile lyase-like
Location	Chr05: 426924 .. 428696 (-)
RNA-Seq Expression	Lcy05g000300
Synteny	Lcy05g000300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCGCCGCATCTTCTTCCACTCCCAATGCCTCGCGTTCTTGATGATCATCTTTTACCGCTCAACCTCCATCGTCTCCGCCGATCCAAACTCCTCTCAACAAGGTAATTCAATTCATTTTCAAACAAATTTCCATCTCCATTATCGTGTACAGGAATTTTCTTGAATGTTTAAAATCGAAAAACCCTAATTATTGCTTACAGAGCCCGGTTACCTGAAGTTCGTCTCGAACGCGACGGAATTTCCAGCAGAAGATTACTACGACTACATAATCGTTGGCGGAGGAACAGCCGGTTGCCCGTTGGCCGCCACATTATCTAGCAAATTCCGAGTTCTTGTACTGGAGCGAGGCGGCGTTCCGCATGAAAACTCGAATTTAATGTCACAAGAAGGATTCTTGACGACGCTAATGGACGACGTCCACTCACGTGACTCCCCCGTGCAAGCCTTCACCTCCGAAGAAGGAGTCCCCAACGCACGTGGCCGGATCCTCGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCCAAAATTCTCACCTCAACTGGGACCTCAAAATCGTGAACCAGTCGTACGAGTGGGTCGAGAAGGCGATCGTATTCAGGCCAAATCTCAAGAACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAACCCTTACAACGGCTTCAGTCTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAATGGGCCGACGCCACAGCGCCGCGGATCTTCTCTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGCACGCGACGGTGGAGAGAATTCTCTTGGCTTCAACGGGACCGTTAAGACGTTCGGCAATCGGCGTTCTTTTTCGTGACCAAATGGGAGCGTATCACCACGCGATGTTACGCGCGCGTGGGGAAGTGTTGGTATCAGCAGGCGCAATAGGGAGTCCACAGTTACTGTTACTCAGTGGAATCGGTTCACGTCCTTATCTTTCGTCATGGGGAATTCCAGTGACTCATCATTTGCCCTACGTCGGGCATTACCTTTACGACAATCCCCGTAACGGAATTTCCATACTGGCGCCGATGCCGTTAGAGCAGTCTCTGATACAAGTAGCGGGAATAACGGAATCGGGCGCGTATCTGGAAGCGGCGTCGAACGTAATCCCGTTCACTTCACCGCCGAGGTCTTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGACGGTGGCGACGCTGATGGAGAAGATAGTCGGGCCGGTGTCGTCGGGTTCGATGCGGCTGGCGTCGACGGACGTGAGGGTGAACCCGGTGGTCCGGTTCAACTACTTCAGCAACGCGGTGGATTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGGTGCTGAGGAGCCGGTCGATGGAGAGCTTTAAGTTTGGGGGGTGGTTCGGGAGGAGGGAGTTCCGGTTCGTGGGGCCGGTTTACCCGGAGGATGAAGGGAACGAAGGATTGGTGAGGGAGTTCTGTAGAAGGAGTGTGAGCACGATATGGCATTACCATGGAGGGTGTGTGGTGGGGAAAGTGGTGGAGCGTAATTATCGAGTGATTGGAATGGAAGCGCTGAGAGTTGTCGACGGATCGACTTTTGGGGTTTCGCCGGGTACTAATCCACAGGCCACGCTCATGATGCTTGGACGATATGTCGGTTTGCAAATTATCAAGGAACGCTCCTCCACTACTGCTACTCGTCTATAG

mRNA sequence

ATGAATCGCCGCATCTTCTTCCACTCCCAATGCCTCGCGTTCTTGATGATCATCTTTTACCGCTCAACCTCCATCGTCTCCGCCGATCCAAACTCCTCTCAACAAGAGCCCGGTTACCTGAAGTTCGTCTCGAACGCGACGGAATTTCCAGCAGAAGATTACTACGACTACATAATCGTTGGCGGAGGAACAGCCGGTTGCCCGTTGGCCGCCACATTATCTAGCAAATTCCGAGTTCTTGTACTGGAGCGAGGCGGCGTTCCGCATGAAAACTCGAATTTAATGTCACAAGAAGGATTCTTGACGACGCTAATGGACGACGTCCACTCACGTGACTCCCCCGTGCAAGCCTTCACCTCCGAAGAAGGAGTCCCCAACGCACGTGGCCGGATCCTCGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCCAAAATTCTCACCTCAACTGGGACCTCAAAATCGTGAACCAGTCGTACGAGTGGGTCGAGAAGGCGATCGTATTCAGGCCAAATCTCAAGAACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAACCCTTACAACGGCTTCAGTCTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAATGGGCCGACGCCACAGCGCCGCGGATCTTCTCTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGCACGCGACGGTGGAGAGAATTCTCTTGGCTTCAACGGGACCGTTAAGACGTTCGGCAATCGGCGTTCTTTTTCGTGACCAAATGGGAGCGTATCACCACGCGATGTTACGCGCGCGTGGGGAAGTGTTGGTATCAGCAGGCGCAATAGGGAGTCCACAGTTACTGTTACTCAGTGGAATCGGTTCACGTCCTTATCTTTCGTCATGGGGAATTCCAGTGACTCATCATTTGCCCTACGTCGGGCATTACCTTTACGACAATCCCCGTAACGGAATTTCCATACTGGCGCCGATGCCGTTAGAGCAGTCTCTGATACAAGTAGCGGGAATAACGGAATCGGGCGCGTATCTGGAAGCGGCGTCGAACGTAATCCCGTTCACTTCACCGCCGAGGTCTTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGACGGTGGCGACGCTGATGGAGAAGATAGTCGGGCCGGTGTCGTCGGGTTCGATGCGGCTGGCGTCGACGGACGTGAGGGTGAACCCGGTGGTCCGGTTCAACTACTTCAGCAACGCGGTGGATTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGGTGCTGAGGAGCCGGTCGATGGAGAGCTTTAAGTTTGGGGGGTGGTTCGGGAGGAGGGAGTTCCGGTTCGTGGGGCCGGTTTACCCGGAGGATGAAGGGAACGAAGGATTGGTGAGGGAGTTCTGTAGAAGGAGTGTGAGCACGATATGGCATTACCATGGAGGGTGTGTGGTGGGGAAAGTGGTGGAGCGTAATTATCGAGTGATTGGAATGGAAGCGCTGAGAGTTGTCGACGGATCGACTTTTGGGGTTTCGCCGGGTACTAATCCACAGGCCACGCTCATGATGCTTGGACGATATGTCGGTTTGCAAATTATCAAGGAACGCTCCTCCACTACTGCTACTCGTCTATAG

Coding sequence (CDS)

ATGAATCGCCGCATCTTCTTCCACTCCCAATGCCTCGCGTTCTTGATGATCATCTTTTACCGCTCAACCTCCATCGTCTCCGCCGATCCAAACTCCTCTCAACAAGAGCCCGGTTACCTGAAGTTCGTCTCGAACGCGACGGAATTTCCAGCAGAAGATTACTACGACTACATAATCGTTGGCGGAGGAACAGCCGGTTGCCCGTTGGCCGCCACATTATCTAGCAAATTCCGAGTTCTTGTACTGGAGCGAGGCGGCGTTCCGCATGAAAACTCGAATTTAATGTCACAAGAAGGATTCTTGACGACGCTAATGGACGACGTCCACTCACGTGACTCCCCCGTGCAAGCCTTCACCTCCGAAGAAGGAGTCCCCAACGCACGTGGCCGGATCCTCGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCCAAAATTCTCACCTCAACTGGGACCTCAAAATCGTGAACCAGTCGTACGAGTGGGTCGAGAAGGCGATCGTATTCAGGCCAAATCTCAAGAACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAACCCTTACAACGGCTTCAGTCTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAATGGGCCGACGCCACAGCGCCGCGGATCTTCTCTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGCACGCGACGGTGGAGAGAATTCTCTTGGCTTCAACGGGACCGTTAAGACGTTCGGCAATCGGCGTTCTTTTTCGTGACCAAATGGGAGCGTATCACCACGCGATGTTACGCGCGCGTGGGGAAGTGTTGGTATCAGCAGGCGCAATAGGGAGTCCACAGTTACTGTTACTCAGTGGAATCGGTTCACGTCCTTATCTTTCGTCATGGGGAATTCCAGTGACTCATCATTTGCCCTACGTCGGGCATTACCTTTACGACAATCCCCGTAACGGAATTTCCATACTGGCGCCGATGCCGTTAGAGCAGTCTCTGATACAAGTAGCGGGAATAACGGAATCGGGCGCGTATCTGGAAGCGGCGTCGAACGTAATCCCGTTCACTTCACCGCCGAGGTCTTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGACGGTGGCGACGCTGATGGAGAAGATAGTCGGGCCGGTGTCGTCGGGTTCGATGCGGCTGGCGTCGACGGACGTGAGGGTGAACCCGGTGGTCCGGTTCAACTACTTCAGCAACGCGGTGGATTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGGTGCTGAGGAGCCGGTCGATGGAGAGCTTTAAGTTTGGGGGGTGGTTCGGGAGGAGGGAGTTCCGGTTCGTGGGGCCGGTTTACCCGGAGGATGAAGGGAACGAAGGATTGGTGAGGGAGTTCTGTAGAAGGAGTGTGAGCACGATATGGCATTACCATGGAGGGTGTGTGGTGGGGAAAGTGGTGGAGCGTAATTATCGAGTGATTGGAATGGAAGCGCTGAGAGTTGTCGACGGATCGACTTTTGGGGTTTCGCCGGGTACTAATCCACAGGCCACGCTCATGATGCTTGGACGATATGTCGGTTTGCAAATTATCAAGGAACGCTCCTCCACTACTGCTACTCGTCTATAG

Protein sequence

MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFNYFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKERSSTTATRL

Homology

[Close]

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.6e-215
Identity = 356/516 (68.99%), Postives = 437/516 (84.69%), Query Frame = 0


Query: 37  PGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMS 96
           PG+++F+SNAT+F +EDYYDYIIVGGGTAGCPLAATLS  FRVL+LERGGVP+   N+MS
Sbjct: 36  PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95

Query: 97  QEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHL 156
            +GFLTTL  DV++ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF+NS L
Sbjct: 96  HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155

Query: 157 NWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGST 216
            WDL  VNQSYEWVE+AIVFRP L+ WQ+A+RD ++E G++P+NGF+L+H VGTKIGGST
Sbjct: 156 VWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGST 215

Query: 217 FDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPL---RRSAIGVLFRDQMGAY 276
           FD  GRRHS+ADLL YA   NI+VAV+ATVER+LLAS+  +     SAIGV++RDQ+G +
Sbjct: 216 FDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRF 275

Query: 277 HHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNG 336
           HHA++R RGEV++SAGA+GSPQLL LSGIG R YLS+WGIPV    P+VG ++YDNPRNG
Sbjct: 276 HHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNG 335

Query: 337 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPVYLTVATLME 396
           ISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP  S FIR P++P+Y+ V T+ME
Sbjct: 336 ISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIME 395

Query: 397 KIVGPVSSGSMRLASTDVRVNPVVRFNYFSNAVDLERCVNGTRKIGEVLRSRSMESFKFG 456
           KI+GPVS G +RLASTDVR+NPVVRFNYFS+  DLERCVNGTRKIGE+LRSR+M+ F   
Sbjct: 396 KILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR 455

Query: 457 GWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEA 516
            WFG R FRFVG   P D+ N+ ++ +FCRR+VSTIWHYHGG VVGKVV+ + +VIG+ +
Sbjct: 456 EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNS 515

Query: 517 LRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKER 550
           LR+VDGSTF +SPGTNPQATLMMLGRY+GL++++ER
Sbjct: 516 LRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.6e-145
Identity = 261/542 (48.15%), Postives = 364/542 (67.16%), Query Frame = 0


Query: 12  LAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAA 71
           L   + + +   S V +  N+S  +  YL FV +AT+   E  YDYIIVGGGTAGCPLAA
Sbjct: 11  LVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAA 70

Query: 72  TLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRI 131
           TLS+ + VLVLERG +P E  NL+  +GF+  L  +   + +PV+ F SE+G+ N RGR+
Sbjct: 71  TLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRV 130

Query: 132 LGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGM 191
           LGG+S INAG Y RA+ +FF  + + WD+ +VNQ+YEWVE  IVF P+ + WQ+ +    
Sbjct: 131 LGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAY 190

Query: 192 VEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILL 251
           +EAGI P NGFS+DHL GT++ GSTFD  G RH++ +LL+   P N++VAV A VE+I+ 
Sbjct: 191 LEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIF 250

Query: 252 ASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSW 311
            S+     +AIGV++ D  G  H A +R  GEV++SAG IGSPQLLLLSG+G   YL+S 
Sbjct: 251 -SSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSL 310

Query: 312 GIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSP 371
            I V    PYVG Y+YDNPRN I+IL P P+E S + V GIT    + + + + +PF +P
Sbjct: 311 NISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTP 370

Query: 372 PRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRL-ASTDVRVNPVVRFNYFSNAVDLER 431
           P SFF  T       T A ++ K+ GP+S G++ L +S+DVRV P V+FNY+SN  DL  
Sbjct: 371 PFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSH 430

Query: 432 CVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIW 491
           CV+G +K+GEVL + ++E +K     G   F  +G   PE++ ++     FCR SV++ W
Sbjct: 431 CVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYW 490

Query: 492 HYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKERS 551
           HYHGGC+VGKV++  +RV G+ ALRVVDGSTF  +P ++PQ   +MLGRY+G+QI++ERS
Sbjct: 491 HYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERS 548

Query: 552 ST 553
           ++
Sbjct: 551 AS 548

[Close]

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.2e-145
Identity = 256/540 (47.41%), Postives = 374/540 (69.26%), Query Frame = 0


Query: 14  FLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATL 73
           F++++ Y   S V +   +S  +  YL+F  +AT+   E  YDY+IVGGGT+GCPLAATL
Sbjct: 16  FVLLLQY---SEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATL 75

Query: 74  SSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILG 133
           S K++VLVLERG +P    N+++ +GF+  L  +   + +PV+ F SE+G+ N RGR+LG
Sbjct: 76  SEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLG 135

Query: 134 GSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVE 193
           G+S INAG Y+RA+ + +  S ++WD+ +VN++YEWVE  IVF+PN + WQS      +E
Sbjct: 136 GTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLE 195

Query: 194 AGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLAS 253
           AG++P +GFSLDH  GT+I GSTFD  G RH+A +LL+     N++V VHA+VE+I+ ++
Sbjct: 196 AGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSN 255

Query: 254 TGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGI 313
              L  +A GV++RD  G  H A +R++GEV+VSAG IG+PQLLLLSG+G   YLSS  I
Sbjct: 256 APGL--TATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNI 315

Query: 314 PVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPR 373
           PV    PYVG +L+DNPRN I+IL P P+E +++ V GI  S  + + + + +PFT+PP 
Sbjct: 316 PVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTPPF 375

Query: 374 SFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRL-ASTDVRVNPVVRFNYFSNAVDLERCV 433
           SFF  T       T A    K+ GP+S GS+ L +S++VRV+P V+FNY+SN  DL  CV
Sbjct: 376 SFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCV 435

Query: 434 NGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIWHY 493
           +G +KIGE+L + +++ +K     G   F  +G   P+D+ ++     FCR SV++ WHY
Sbjct: 436 SGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHY 495

Query: 494 HGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKERSST 553
           HGGC+VGKV++ ++RV G++ALRVVDGSTF  +P ++PQ   +MLGRYVG++I++ERS++
Sbjct: 496 HGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSAS 547

[Close]

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.1e-144
Identity = 257/545 (47.16%), Postives = 374/545 (68.62%), Query Frame = 0


Query: 12  LAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAA 71
           L   + + +   S V +   +S  +  YL F  +AT+   E  YDY+IVGGGT+GCPLAA
Sbjct: 11  LVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAA 70

Query: 72  TLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRI 131
           TLS K++VLVLERG +P    N+++ +GF+  L  +   + +PV+ F SE+G+ N RGR+
Sbjct: 71  TLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRV 130

Query: 132 LGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGM 191
           LGG+S INAG Y+RA+ + +  S ++WD+ +VNQ+YEWVE  IV++PN ++WQS  +   
Sbjct: 131 LGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAF 190

Query: 192 VEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILL 251
           +EAG++P +GFSLDH  GT+I GSTFD  G RH+A +LL+     N++V VHA+VE+I+ 
Sbjct: 191 LEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIF 250

Query: 252 ASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSW 311
           ++   L  +A GV++RD  G  H A +R++GEV+VSAG IG+PQLLLLSG+G   YLSS 
Sbjct: 251 SNAPGL--TATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 310

Query: 312 GIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSP 371
            IPV    PYVG +L+DNPRN I+IL P P+E +++ V GI  S  + + + + +PFT+P
Sbjct: 311 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTP 370

Query: 372 PRSFFIRTPSAPVYL---TVATLMEKIVGPVSSGSMRL-ASTDVRVNPVVRFNYFSNAVD 431
           P  FF   PSA   L   T A    K+ GP+S GS+ L +S++VRV+P V+FNY+SN  D
Sbjct: 371 PFGFF---PSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTD 430

Query: 432 LERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVS 491
           L  CV+G +KIGE+L + +++ +K     G   F  +G   P+D+ ++     FCR SV+
Sbjct: 431 LSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVA 490

Query: 492 TIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIK 551
           + WHYHGGC+VGKV++ ++RV G+ ALRVVDGSTF  +P ++PQ   +MLGRYVG++I++
Sbjct: 491 SYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQ 547

Query: 552 ERSST 553
           ERS++
Sbjct: 551 ERSAS 547

[Close]

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.4e-141
Identity = 253/536 (47.20%), Postives = 365/536 (68.10%), Query Frame = 0


Query: 16  MIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSS 75
           +++ +   S V +  N+S  +  YLKFV NAT+   E  YDYI++GGGT+GCPLAATLS 
Sbjct: 15  LLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSE 74

Query: 76  KFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGS 135
           K++VL+LERG +  E  N ++ +GF   L      + +PV+ F SE+G+ N R RILGG+
Sbjct: 75  KYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGK-TPVERFVSEDGIDNVRARILGGT 134

Query: 136 SAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAG 195
           + INAG Y+RA+ +F+  + + WDL +VN++YEWVE AIV +PN ++WQS + +G +EAG
Sbjct: 135 TIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAG 194

Query: 196 INPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTG 255
           I P NGFSLDH  GT++ GSTFD  G RH+A +LL+   P N+ VAV A+VE+IL  S+ 
Sbjct: 195 ILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILF-SSN 254

Query: 256 PLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPV 315
               SAIGV++ D  G  H A +R  GEV+VSAG IG+PQLLLLSG+G   YLSS  I V
Sbjct: 255 TSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITV 314

Query: 316 THHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSF 375
               PYVG +LY+NPRN I+   P P+E S++ V GI     Y + + + +PF++PP S 
Sbjct: 315 VQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFSTPPFSL 374

Query: 376 FIRTPSAPVYLTVATLMEKIVGPVSSGSMRL-ASTDVRVNPVVRFNYFSNAVDLERCVNG 435
           F  T       T A ++ ++ GP+S GS+ L +S+DVR+ P ++FNY+SN+ DL  CV+G
Sbjct: 375 FPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSG 434

Query: 436 TRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIWHYHG 495
            +K+G++LR++++E +K     G   F ++G   PE++ ++     FC  +V++ WHYHG
Sbjct: 435 MKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHG 494

Query: 496 GCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKERS 551
           G +VGKV++ ++RV+G++ALRVVD STF   P ++PQ   +MLGRYVGLQI++ERS
Sbjct: 495 GSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

[Close]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL
Match: A0A0A0KCB1 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447930 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.0e-275
Identity = 478/558 (85.66%), Postives = 506/558 (90.68%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RI FHSQ L FL+I      S  SA PNSSQQ+P YLKFVSNATEFP  DYYDYIIV
Sbjct: 1   MESRISFHSQILTFLLIF-----SASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIV 60

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSR+SPVQAFTS
Sbjct: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTS 120

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEGVPNARGRILGGSSAINAGFYSRADQAFF+NS L WDL IVNQSYEWVEK IVF+PNL
Sbjct: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNL 180

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE+GRRHSAADLLS+ATPFNIKV
Sbjct: 181 KNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKV 240

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           A++A VERILLAST   R  AIGVL+RDQ+G YHHAML ARGEV +SAGAIGSPQLLLLS
Sbjct: 241 AIYANVERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLS 300

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESG YLE
Sbjct: 301 GIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLE 360

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           AASNVIPFTSPPRSFFIRTPS PVYLTVATLMEKI GPV+SGSMRLASTDVRVNPVVRFN
Sbjct: 361 AASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFN 420

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLV-R 480
           YF NAVDLERCVNGTRKIGE+LRS++ME FKF  WFGR+EFR+VGP YPE E  +GLV R
Sbjct: 421 YFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLR 480

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFCR+SVSTIWHYHGGCV GKVV+ NYRVIGMEALRVVDGS FGVSPGTNPQAT+MMLGR
Sbjct: 481 EFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGR 540

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQII +RS+ T  R+
Sbjct: 541 HVGLQIINQRSAVTTHRV 553

[Close]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL
Match: A0A6J1GR64 ((R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.7e-275
Identity = 476/558 (85.30%), Postives = 512/558 (91.76%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIF +SQ LAFL ++FY S + +SAD +SSQ+EP YLKFVS+A +FP EDYYDYIIV
Sbjct: 18  MPPRIFSYSQILAFL-LLFYASPASISADLSSSQREPSYLKFVSDAADFPKEDYYDYIIV 77

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 78  GGGTAGCPLAATLSSKFRVLVLERGGVPHANSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 137

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEG+PNARGRILGGSSAINAGFYSRAD AFFQNS L+WDL +VNQSYEWVEK IVFRPNL
Sbjct: 138 EEGIPNARGRILGGSSAINAGFYSRADPAFFQNSRLHWDLAMVNQSYEWVEKEIVFRPNL 197

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PY+GFS DHL+GTKIGGSTFDE+GRRHSAADLL+YA P NIKV
Sbjct: 198 KNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCNIKV 257

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           AVHATVERIL+AS    + +A GVL+ DQ GAYHHA+LRARGEVLV+AGAIGSP LLLLS
Sbjct: 258 AVHATVERILVAS----KAAATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSPHLLLLS 317

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITESGAYLE
Sbjct: 318 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITESGAYLE 377

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           A SNVIPFTSPP+SFFIR  SAPVYLTVATLMEKI+GPVS GSMRLASTDVRVNPVV FN
Sbjct: 378 ATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVNPVVWFN 437

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPE-DEGNEGLVR 480
           YF NAVDLERCVNGTRKIGEVLRSRSME FKF GW  +REFRFVGPVYPE +E ++G +R
Sbjct: 438 YFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEKDDGFLR 497

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFC+R+VSTIWHYHGGCV GKVV+RN+RVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR
Sbjct: 498 EFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 557

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQIIKERSSTT  R+
Sbjct: 558 HVGLQIIKERSSTTTHRV 570

[Close]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL
Match: A0A6J1JYK6 ((R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.5e-274
Identity = 476/558 (85.30%), Postives = 511/558 (91.58%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIF +SQ LAFL ++FY S + +SAD +SSQ+EP YLKFVS+A +FP EDYYDYIIV
Sbjct: 18  MPPRIFSYSQILAFL-LLFYASPASISADLSSSQREPSYLKFVSDAADFPKEDYYDYIIV 77

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 78  GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 137

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEG+PNARGRILGGSSAINAGFYSRAD AFFQNSHL WDL +VNQSYEWVEK IVFRPNL
Sbjct: 138 EEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLYWDLAMVNQSYEWVEKEIVFRPNL 197

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PY+GFS DHL+GTKIGGSTFDE+GRRHSAADLL+YA P NIKV
Sbjct: 198 KNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCNIKV 257

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           AVHATVERIL+AS    + +A GVL+ DQ GAYHHA+LRARGEVLV+AGAIGSPQLLLLS
Sbjct: 258 AVHATVERILVAS----KATATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSPQLLLLS 317

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITESGAYLE
Sbjct: 318 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITESGAYLE 377

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           A SNVIPFTSPP+SFFIR  SAPVYLTVATLMEKI+GPVS GSMRLASTDVRVNPVV FN
Sbjct: 378 ATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVNPVVWFN 437

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPE-DEGNEGLVR 480
           YF NAVDLERCVNGTRKIGEVLRSRSME FKF GW  +REFRFVGPVYPE +E ++G +R
Sbjct: 438 YFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEKDDGFLR 497

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFC+R+VSTIWHYHGGCV GKVV+RN+RVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR
Sbjct: 498 EFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 557

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQIIKERS T   R+
Sbjct: 558 HVGLQIIKERSFTATHRV 570

[Close]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL
Match: A0A1S3CHZ4 ((R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.3e-273
Identity = 472/558 (84.59%), Postives = 504/558 (90.32%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIFFHSQ L FL+I      S  SA PNSSQ++P YLKFVSNATEFP  DYYDYIIV
Sbjct: 19  MASRIFFHSQILTFLLIF-----SASSALPNSSQKDPDYLKFVSNATEFPKVDYYDYIIV 78

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLS+KFRVLVLERGG PH NSNLMSQEGFLTTLMDDVHSR SPVQAFTS
Sbjct: 79  GGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAFTS 138

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEGVPNARGRILGGSSAINAGFYSRADQAFF+NS L WDL IVNQSYEWVEK IVF+PNL
Sbjct: 139 EEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNL 198

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE+GRRHSAADLLSYATPFNIKV
Sbjct: 199 KNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNIKV 258

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           A++ATVERILL ST   +  AIGVL+RDQ+G YHHAML ARGEV +SAGAIGSPQLLLLS
Sbjct: 259 AIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQLLLLS 318

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITESG YLE
Sbjct: 319 GIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITESGTYLE 378

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           AASNVIPFT+PPRSFFIRTPS PVYLTVATLMEKI GPV+SG+MRLASTDVRVNPVVRFN
Sbjct: 379 AASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNPVVRFN 438

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLV-R 480
           YF NAVDLERCVNGTRKIG++LRS++ME FKF  WFGR+EFRFVGP YPED   +GLV R
Sbjct: 439 YFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDEDGLVLR 498

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFCR+SVSTIWHYHGGCV GKVV+ NYRV+GMEALRVVDGS FGVSPGTNPQAT+MMLGR
Sbjct: 499 EFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATVMMLGR 558

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQII +RS+ T  R+
Sbjct: 559 HVGLQIINQRSAVTTHRV 571

[Close]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL
Match: A0A5D3C0K1 ((R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001740 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.6e-273
Identity = 471/554 (85.02%), Postives = 502/554 (90.61%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIFFHSQ L FL+I      S  SA PNSSQ++P YLKFVSNATEFP  DYYDYIIV
Sbjct: 19  MASRIFFHSQILTFLLIF-----SASSALPNSSQKDPDYLKFVSNATEFPKVDYYDYIIV 78

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLS+KFRVLVLERGG PH NSNLMSQEGFLTTLMDDVHSR SPVQAFTS
Sbjct: 79  GGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAFTS 138

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEGVPNARGRILGGSSAINAGFYSRADQAFF+NS L WDL IVNQSYEWVEK IVF+PNL
Sbjct: 139 EEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNL 198

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE+GRRHSAADLLSYATPFNIKV
Sbjct: 199 KNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNIKV 258

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           A++ATVERILL ST   +  AIGVL+RDQ+G YHHAML ARGEV +SAGAIGSPQLLLLS
Sbjct: 259 AIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQLLLLS 318

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITESG YLE
Sbjct: 319 GIGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITESGTYLE 378

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           AASNVIPFT+PPRSFFIRTPS PVYLTVATLMEKI GPV+SG+MRLASTDVRVNPVVRFN
Sbjct: 379 AASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNPVVRFN 438

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLV-R 480
           YF NAVDLERCVNGTRKIG++LRS++ME FKF  WFGR+EFRFVGP YPED   +GLV R
Sbjct: 439 YFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDEDGLVLR 498

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFCR+SVSTIWHYHGGCV GKVV+ NYRV+GMEALRVVDGS FGVSPGTNPQAT+MMLGR
Sbjct: 499 EFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATVMMLGR 558

Query: 541 YVGLQIIKERSSTT 554
           +VGLQII +RS+ T
Sbjct: 559 HVGLQIINQRSAVT 567

[Close]

BLAST of Lcy05g000300 vs. NCBI nr
Match: XP_038899448.1 ((R)-mandelonitrile lyase-like [Benincasa hispida])

HSP 1 Score: 988.4 bits (2554), Expect = 2.5e-284
Identity = 498/559 (89.09%), Postives = 518/559 (92.67%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  R FFHSQ LAFL +IFY S     A PNSSQ+EP YLKFV+NATEFP EDYYDYIIV
Sbjct: 19  MAPRFFFHSQFLAFL-LIFYAS----FAHPNSSQREPDYLKFVANATEFPEEDYYDYIIV 78

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 79  GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 138

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEGVPNARGRILGGSSAINAGFYSRADQAFF+NS LNWDL +VNQSYEWVEK IVFRPNL
Sbjct: 139 EEGVPNARGRILGGSSAINAGFYSRADQAFFKNSRLNWDLAMVNQSYEWVEKEIVFRPNL 198

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVE GINPYNGFSLDHL+GTKIGGSTFDE+GRRHSAADLLSYATP NIKV
Sbjct: 199 KNWQSAVRDGMVEVGINPYNGFSLDHLIGTKIGGSTFDELGRRHSAADLLSYATPINIKV 258

Query: 241 AVHATVERILLASTGPLRR-SAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLL 300
           AV+ATVERILLAS GPLRR +AIGVL+RDQ GAYHHAMLRARGEVLVSAGAIGSPQLLLL
Sbjct: 259 AVYATVERILLASGGPLRRTTAIGVLYRDQKGAYHHAMLRARGEVLVSAGAIGSPQLLLL 318

Query: 301 SGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYL 360
           SGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYL
Sbjct: 319 SGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYL 378

Query: 361 EAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRF 420
           EAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKI GPVSSGSMRLASTDVRVNPVVRF
Sbjct: 379 EAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKITGPVSSGSMRLASTDVRVNPVVRF 438

Query: 421 NYFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEG-LV 480
           NYF NAVDLERCVNGTRKIGE+LRSRSME FKF  W GR EFRFVGPVYPE+E  +G ++
Sbjct: 439 NYFGNAVDLERCVNGTRKIGEILRSRSMEGFKFRDWLGRSEFRFVGPVYPENEDEDGSML 498

Query: 481 REFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLG 540
           REFCRRSVSTIWHYHGGCV GKVV+ N RVIGMEALRVVDGS FGVSPGTNPQATLMMLG
Sbjct: 499 REFCRRSVSTIWHYHGGCVAGKVVDSNSRVIGMEALRVVDGSIFGVSPGTNPQATLMMLG 558

Query: 541 RYVGLQIIKERSSTTATRL 558
           R+VGLQII +RS+ T  R+
Sbjct: 559 RHVGLQIINQRSAITTHRI 572

[Close]

BLAST of Lcy05g000300 vs. NCBI nr
Match: KGN45431.1 (hypothetical protein Csa_016192 [Cucumis sativus])

HSP 1 Score: 958.7 bits (2477), Expect = 2.1e-275
Identity = 478/558 (85.66%), Postives = 506/558 (90.68%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RI FHSQ L FL+I      S  SA PNSSQQ+P YLKFVSNATEFP  DYYDYIIV
Sbjct: 1   MESRISFHSQILTFLLIF-----SASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIV 60

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSR+SPVQAFTS
Sbjct: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTS 120

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEGVPNARGRILGGSSAINAGFYSRADQAFF+NS L WDL IVNQSYEWVEK IVF+PNL
Sbjct: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNL 180

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE+GRRHSAADLLS+ATPFNIKV
Sbjct: 181 KNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKV 240

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           A++A VERILLAST   R  AIGVL+RDQ+G YHHAML ARGEV +SAGAIGSPQLLLLS
Sbjct: 241 AIYANVERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLS 300

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESG YLE
Sbjct: 301 GIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLE 360

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           AASNVIPFTSPPRSFFIRTPS PVYLTVATLMEKI GPV+SGSMRLASTDVRVNPVVRFN
Sbjct: 361 AASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFN 420

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPEDEGNEGLV-R 480
           YF NAVDLERCVNGTRKIGE+LRS++ME FKF  WFGR+EFR+VGP YPE E  +GLV R
Sbjct: 421 YFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLR 480

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFCR+SVSTIWHYHGGCV GKVV+ NYRVIGMEALRVVDGS FGVSPGTNPQAT+MMLGR
Sbjct: 481 EFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGR 540

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQII +RS+ T  R+
Sbjct: 541 HVGLQIINQRSAVTTHRV 553

[Close]

BLAST of Lcy05g000300 vs. NCBI nr
Match: KAG7013945.1 ((R)-mandelonitrile lyase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 958.7 bits (2477), Expect = 2.1e-275
Identity = 477/558 (85.48%), Postives = 513/558 (91.94%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIF +SQ LAFL ++FY S + +SAD +SSQ+EP YLKFVS+A +FP EDYYDYIIV
Sbjct: 1   MPPRIFSYSQILAFL-LLFYASPASISADLSSSQREPSYLKFVSDAADFPKEDYYDYIIV 60

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHANSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEG+PNARGRILGGSSAINAGFYSRAD AFFQNSHL+WDL +VNQSYEWVEK IVFRPNL
Sbjct: 121 EEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLHWDLAMVNQSYEWVEKEIVFRPNL 180

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PY+GFS DHL+GTKIGGSTFDE+GRRHSAADLL+YA P NIKV
Sbjct: 181 KNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCNIKV 240

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           AVHATVERIL+AS    + +A GVL+ DQ GAYHHA+LRARGEVLV+AGAIGSP LLLLS
Sbjct: 241 AVHATVERILVAS----KATATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSPHLLLLS 300

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITESGAYLE
Sbjct: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITESGAYLE 360

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           A SNVIPFTSPP+SFFIR  SAPVYLTVATLMEKI+GPVS GSMRLASTDVRVNPVV FN
Sbjct: 361 ATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVNPVVWFN 420

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPE-DEGNEGLVR 480
           YF NAVDLERCVNGTRKIGEVLRSRSME FKF GW  +REFRFVGPVYPE +E ++G +R
Sbjct: 421 YFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEKDDGFLR 480

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFC+R+VSTIWHYHGGCV GKVV+RN+RVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR
Sbjct: 481 EFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQIIKERSSTT  R+
Sbjct: 541 HVGLQIIKERSSTTTHRV 553

[Close]

BLAST of Lcy05g000300 vs. NCBI nr
Match: XP_023549158.1 ((R)-mandelonitrile lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 958.4 bits (2476), Expect = 2.8e-275
Identity = 477/558 (85.48%), Postives = 513/558 (91.94%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIF +SQ LAFL ++FY S + +SAD +SSQ+EP YLKFVS+A +FP EDYYDYIIV
Sbjct: 18  MPPRIFSYSQILAFL-LLFYASPASISADLSSSQREPSYLKFVSDAADFPKEDYYDYIIV 77

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 78  GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 137

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEG+PNARGRILGGSSAINAGFYSRAD AFFQNSHL+WDL +VNQSYEWVEK IVFRPNL
Sbjct: 138 EEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLHWDLAMVNQSYEWVEKEIVFRPNL 197

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PY+GFS DHL+GTKIGGSTFDE+GRRHSAADLL+YA P NIKV
Sbjct: 198 KNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCNIKV 257

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           AVHATVERIL+AS    + +A GVL+ DQ GAYHHA+LRARGEVLV+AGAIGSP LLLLS
Sbjct: 258 AVHATVERILVAS----KATATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSPHLLLLS 317

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITESGAYLE
Sbjct: 318 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITESGAYLE 377

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           A SNVIPFTSPP+SFFIR  SAPVYLTVATLMEKI+GPVS GSMRLASTDVRVNPVV FN
Sbjct: 378 ATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVNPVVWFN 437

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPE-DEGNEGLVR 480
           YF NAVDLERCVNGTRKIGEVLRSRSME FKF GW  +REFRFVGPVYPE +E ++G +R
Sbjct: 438 YFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEKDDGFLR 497

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFC+R+VSTIWHYHGGCV GKVV+RN+RVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR
Sbjct: 498 EFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 557

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQIIKERSSTT  R+
Sbjct: 558 HVGLQIIKERSSTTTHRV 570

[Close]

BLAST of Lcy05g000300 vs. NCBI nr
Match: XP_022953985.1 ((R)-mandelonitrile lyase-like [Cucurbita moschata])

HSP 1 Score: 955.7 bits (2469), Expect = 1.8e-274
Identity = 476/558 (85.30%), Postives = 512/558 (91.76%), Query Frame = 0


Query: 1   MNRRIFFHSQCLAFLMIIFYRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIV 60
           M  RIF +SQ LAFL ++FY S + +SAD +SSQ+EP YLKFVS+A +FP EDYYDYIIV
Sbjct: 18  MPPRIFSYSQILAFL-LLFYASPASISADLSSSQREPSYLKFVSDAADFPKEDYYDYIIV 77

Query: 61  GGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 120
           GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTTLMDDVHSRDSPVQAFTS
Sbjct: 78  GGGTAGCPLAATLSSKFRVLVLERGGVPHANSNLMSQEGFLTTLMDDVHSRDSPVQAFTS 137

Query: 121 EEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNL 180
           EEG+PNARGRILGGSSAINAGFYSRAD AFFQNS L+WDL +VNQSYEWVEK IVFRPNL
Sbjct: 138 EEGIPNARGRILGGSSAINAGFYSRADPAFFQNSRLHWDLAMVNQSYEWVEKEIVFRPNL 197

Query: 181 KNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKV 240
           KNWQSAVRDGMVEAGI PY+GFS DHL+GTKIGGSTFDE+GRRHSAADLL+YA P NIKV
Sbjct: 198 KNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRRHSAADLLAYAKPCNIKV 257

Query: 241 AVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 300
           AVHATVERIL+AS    + +A GVL+ DQ GAYHHA+LRARGEVLV+AGAIGSP LLLLS
Sbjct: 258 AVHATVERILVAS----KAAATGVLYHDQRGAYHHALLRARGEVLVAAGAIGSPHLLLLS 317

Query: 301 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISIL+PMPLEQSLIQVAGITESGAYLE
Sbjct: 318 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLEQSLIQVAGITESGAYLE 377

Query: 361 AASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKIVGPVSSGSMRLASTDVRVNPVVRFN 420
           A SNVIPFTSPP+SFFIR  SAPVYLTVATLMEKI+GPVS GSMRLASTDVRVNPVV FN
Sbjct: 378 ATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGSMRLASTDVRVNPVVWFN 437

Query: 421 YFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVGPVYPE-DEGNEGLVR 480
           YF NAVDLERCVNGTRKIGEVLRSRSME FKF GW  +REFRFVGPVYPE +E ++G +R
Sbjct: 438 YFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRFVGPVYPEKEEKDDGFLR 497

Query: 481 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 540
           EFC+R+VSTIWHYHGGCV GKVV+RN+RVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR
Sbjct: 498 EFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 557

Query: 541 YVGLQIIKERSSTTATRL 558
           +VGLQIIKERSSTT  R+
Sbjct: 558 HVGLQIIKERSSTTTHRV 570

[Close]

BLAST of Lcy05g000300 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 749.6 bits (1934), Expect = 1.8e-216
Identity = 356/516 (68.99%), Postives = 437/516 (84.69%), Query Frame = 0


Query: 37  PGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMS 96
           PG+++F+SNAT+F +EDYYDYIIVGGGTAGCPLAATLS  FRVL+LERGGVP+   N+MS
Sbjct: 36  PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95

Query: 97  QEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHL 156
            +GFLTTL  DV++ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF+NS L
Sbjct: 96  HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155

Query: 157 NWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGST 216
            WDL  VNQSYEWVE+AIVFRP L+ WQ+A+RD ++E G++P+NGF+L+H VGTKIGGST
Sbjct: 156 VWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGST 215

Query: 217 FDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPL---RRSAIGVLFRDQMGAY 276
           FD  GRRHS+ADLL YA   NI+VAV+ATVER+LLAS+  +     SAIGV++RDQ+G +
Sbjct: 216 FDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRF 275

Query: 277 HHAMLRARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNG 336
           HHA++R RGEV++SAGA+GSPQLL LSGIG R YLS+WGIPV    P+VG ++YDNPRNG
Sbjct: 276 HHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNG 335

Query: 337 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPVYLTVATLME 396
           ISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP  S FIR P++P+Y+ V T+ME
Sbjct: 336 ISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIME 395

Query: 397 KIVGPVSSGSMRLASTDVRVNPVVRFNYFSNAVDLERCVNGTRKIGEVLRSRSMESFKFG 456
           KI+GPVS G +RLASTDVR+NPVVRFNYFS+  DLERCVNGTRKIGE+LRSR+M+ F   
Sbjct: 396 KILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR 455

Query: 457 GWFGRREFRFVGPVYPEDEGNEGLVREFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEA 516
            WFG R FRFVG   P D+ N+ ++ +FCRR+VSTIWHYHGG VVGKVV+ + +VIG+ +
Sbjct: 456 EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNS 515

Query: 517 LRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIKER 550
           LR+VDGSTF +SPGTNPQATLMMLGRY+GL++++ER
Sbjct: 516 LRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of Lcy05g000300 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 466.1 bits (1198), Expect = 4.0e-131
Identity = 251/550 (45.64%), Postives = 343/550 (62.36%), Query Frame = 0


Query: 32  SSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHEN 91
           SS + P Y  F+ +AT  P   YYDYII+GGGTAGCPLAATLS    VL+LERG  P+ N
Sbjct: 24  SSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNN 83

Query: 92  SNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFF 151
            N+     F   L D   S  SP Q F SE+GV NAR R+LGG SA+NAGFY+RA   + 
Sbjct: 84  PNITRLSAFGAALSD--LSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYV 143

Query: 152 QNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTK 211
           +N  + WD  + N+SY+WVE  + F+P +  WQ+AVRDG++EAGI P NGF+ DH+ GTK
Sbjct: 144 RN--MGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTK 203

Query: 212 IGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPLRRSAIGVLFRDQMG 271
            GG+ FD  G RH+AADLL YA P  I V +HATV RIL  + G  +  A GV++RD+ G
Sbjct: 204 FGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTG 263

Query: 272 AYHHAMLR--ARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDN 331
             H A L+  A  E+++SAG +GSPQLL+LSG+G    L +  I V    P+VG  +YDN
Sbjct: 264 QAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDN 323

Query: 332 PRNGISILAPMPLEQSLIQVAGITESGAYLEAA---------------SNVIPFTS--PP 391
           P N + + +P+P+E SLI+V GIT  G Y+EAA               S+   + +   P
Sbjct: 324 PMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSP 383

Query: 392 RSFFIRTPSAPVYLTV-----ATLMEKIVGPVSSGSMRLASTDVRVNPVVRFNYFSNAVD 451
           R+  + + S     +        L+EK++GP+S+G + L + + + NP+V FNYF +  D
Sbjct: 384 RATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDD 443

Query: 452 LERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVG--PVY---PEDEGNEGL---VR 511
           L+RCV G + I  V++S++   +K+               PV    P       L     
Sbjct: 444 LKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLNLTASTPVNLRPPRSGPGASLPPSAE 503

Query: 512 EFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGR 550
           EFC+ +V+TIWHYHGGCVVG+VV+ +Y+VIG++ LRV+D ST G  PGTNPQAT+MMLGR
Sbjct: 504 EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGR 563

[Close]

BLAST of Lcy05g000300 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.7e-127
Identity = 257/557 (46.14%), Postives = 339/557 (60.86%), Query Frame = 0


Query: 33  SQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHENS 92
           S  + GY  F+ +AT  P    +DYII+GGGT+GC LAATLS    VLVLERGG P++N 
Sbjct: 24  SPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNP 83

Query: 93  NLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFQ 152
                E F TTL +   S  S  Q F SE+GV N R R+LGG S +NAGFY+RA   + +
Sbjct: 84  TATDIENFATTLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVK 143

Query: 153 NSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTKI 212
            +   W    V  +YEWVEK + F+P +  WQ+A +DG++EAG  PYNGF+ DH+ GTKI
Sbjct: 144 ET--EWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKI 203

Query: 213 GGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPLRRSAIGVLFRDQMGA 272
           GG+ FD  G RH+AADLL YA P NI V +HA+V +IL  + G  R  A GV+F+D  G 
Sbjct: 204 GGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGV 263

Query: 273 YHHAML--RARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGI-PVTHHLPYVGHYLYDN 332
            H A L   +  EV++SAGAIGSPQLL+LSGIG   +L++ GI P+    P VG  + DN
Sbjct: 264 LHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDN 323

Query: 333 PRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFF------------I 392
           P N I I +P P+E SLIQV GIT+  +Y+E AS VI   S  R FF             
Sbjct: 324 PMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTS 383

Query: 393 RTPS--APVYLT-------------------VATLMEKIVGPVSSGSMRLASTDVRVNPV 452
           RT S  +P   T                      +++KI GP+S G + L +T+   NP 
Sbjct: 384 RTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPS 443

Query: 453 VRFNYFSNAVDLERCVNGTRKIGEVLRSRSMESFKF--GGWFGRREFRFVGP--VYPEDE 512
           VRFNY+    DL+ CV G   I +V+ S++   FK+      G  +     P  + P   
Sbjct: 444 VRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHI 503

Query: 513 GNEGLVREFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQA 550
            +   +R+FC  +V TIWHYHGGC VG+VV++NYRV+G+++LRV+DGSTF  SPGTNPQA
Sbjct: 504 TSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQA 563

[Close]

BLAST of Lcy05g000300 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 448.4 bits (1152), Expect = 8.7e-126
Identity = 252/573 (43.98%), Postives = 343/573 (59.86%), Query Frame = 0


Query: 20  YRSTSIVSADPNSSQQEPGYLKFVSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRV 79
           YR T I  A   SS        F SN      +  YDYI++GGGTAGCPLAATLS  F V
Sbjct: 35  YRYTFIDKASTFSSSSSS---SFSSNG----QDSSYDYIVIGGGTAGCPLAATLSQNFSV 94

Query: 80  LVLERGGVPHENSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAIN 139
           LVLERGGVP  N+N+     F   L D   S  S  QAF S +GV NAR R+LGG S IN
Sbjct: 95  LVLERGGVPFTNANVSFLRNFHIGLAD--ISASSASQAFVSTDGVYNARARVLGGGSCIN 154

Query: 140 AGFYSRADQAFFQNSHLNWDLKIVNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPY 199
           AGFYSRAD AF + +   WD K+V +SY WVE+ IV +P L  WQ A+RD ++E G+ P+
Sbjct: 155 AGFYSRADAAFVKRA--GWDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPF 214

Query: 200 NGFSLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIKVAVHATVERILLASTGPLRR 259
           NGF+ DH+ GTKIGG+ FD  GRRH+AA+LL+YA P  ++V ++ATV++I+  ++G  R 
Sbjct: 215 NGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSG-TRP 274

Query: 260 SAIGVLFRDQMGAYHHAML--RARGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGIPVTH 319
              GV+F+D+ G  H A+L  R   EV++S+GAIGSPQ+L+LSGIG +  L    IPV  
Sbjct: 275 RVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVL 334

Query: 320 HLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFFI 379
              +VG  + DNP N I + +  P+EQSLIQ  GIT+ G Y+EA++    F   P S   
Sbjct: 335 ENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTG---FGQSPESIHT 394

Query: 380 --------------------RTPSAPVYLT-----------VATLMEKIVGPVSSGSMRL 439
                               R  +   Y+T            + ++EK+  P+S G + L
Sbjct: 395 HYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSL 454

Query: 440 ASTDVRVNPVVRFNYFSNAVDLERCVNGTRKIGEVLRSRSMESFKFGGWFGRREFRFVG- 499
            +T+V  NP V FNYF + VDL+RCV   R + +V+ S    ++         +   +  
Sbjct: 455 VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSV 514

Query: 500 ----PVYPEDEGNEGLVREFCRRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGST 555
                + P+   +   + +FC+ +V TIWHYHGGC+VGKVV  N +V+G++ LRV+DGST
Sbjct: 515 KANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGST 574

[Close]

BLAST of Lcy05g000300 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 444.9 bits (1143), Expect = 9.6e-125
Identity = 253/547 (46.25%), Postives = 334/547 (61.06%), Query Frame = 0


Query: 43  VSNATEFPAEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHENSNLMSQEGFLT 102
           + +AT  P    +DYII+GGGT+GC LAATLS    VLVLERGG P++N      E F T
Sbjct: 1   MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60

Query: 103 TLMDDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFQNSHLNWDLKI 162
           TL +   S  S  Q F SE+GV N R R+LGG S +NAGFY+RA   + + +   W    
Sbjct: 61  TLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--EWKTDE 120

Query: 163 VNQSYEWVEKAIVFRPNLKNWQSAVRDGMVEAGINPYNGFSLDHLVGTKIGGSTFDEMGR 222
           V  +YEWVEK + F+P +  WQ+A +DG++EAG  PYNGF+ DH+ GTKIGG+ FD  G 
Sbjct: 121 VEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGH 180

Query: 223 RHSAADLLSYATPFNIKVAVHATVERILLASTGPLRRSAIGVLFRDQMGAYHHAML--RA 282
           RH+AADLL YA P NI V +HA+V +IL  + G  R  A GV+F+D  G  H A L   +
Sbjct: 181 RHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNS 240

Query: 283 RGEVLVSAGAIGSPQLLLLSGIGSRPYLSSWGI-PVTHHLPYVGHYLYDNPRNGISILAP 342
             EV++SAGAIGSPQLL+LSGIG   +L++ GI P+    P VG  + DNP N I I +P
Sbjct: 241 MNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSP 300

Query: 343 MPLEQSLIQVAGITESGAYLEAASNVIPFTSPPRSFF------------IRTPS--APVY 402
            P+E SLIQV GIT+  +Y+E AS VI   S  R FF             RT S  +P  
Sbjct: 301 TPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTL 360

Query: 403 LT-------------------VATLMEKIVGPVSSGSMRLASTDVRVNPVVRFNYFSNAV 462
            T                      +++KI GP+S G + L +T+   NP VRFNY+    
Sbjct: 361 STQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPE 420

Query: 463 DLERCVNGTRKIGEVLRSRSMESFKF--GGWFGRREFRFVGP--VYPEDEGNEGLVREFC 522
           DL+ CV G   I +V+ S++   FK+      G  +     P  + P    +   +R+FC
Sbjct: 421 DLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFC 480

Query: 523 RRSVSTIWHYHGGCVVGKVVERNYRVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVG 550
             +V TIWHYHGGC VG+VV++NYRV+G+++LRV+DGSTF  SPGTNPQAT+MMLGRY+G
Sbjct: 481 IDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMG 540

The following BLAST results are available for this feature:

BLAST of Lcy05g000300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSM2	2.6e-215	68.99	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52707	3.6e-145	48.15	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
P52706	6.2e-145	47.41	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	1.1e-144	47.16	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
O24243	1.4e-141	47.20	(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1	[more]

BLAST of Lcy05g000300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCB1	1.0e-275	85.66	GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447...	[more]
A0A6J1GR64	8.7e-275	85.30	(R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3...	[more]
A0A6J1JYK6	1.5e-274	85.30	(R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 S...	[more]
A0A1S3CHZ4	1.3e-273	84.59	(R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1	[more]
A0A5D3C0K1	3.6e-273	85.02	(R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Lcy05g000300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899448.1	2.5e-284	89.09	(R)-mandelonitrile lyase-like [Benincasa hispida]	[more]
KGN45431.1	2.1e-275	85.66	hypothetical protein Csa_016192 [Cucumis sativus]	[more]
KAG7013945.1	2.1e-275	85.48	(R)-mandelonitrile lyase-like protein, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_023549158.1	2.8e-275	85.48	(R)-mandelonitrile lyase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953985.1	1.8e-274	85.30	(R)-mandelonitrile lyase-like [Cucurbita moschata]	[more]

BLAST of Lcy05g000300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	1.8e-216	68.99	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	4.0e-131	45.64	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	2.7e-127	46.14	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	8.7e-126	43.98	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	9.6e-125	46.25	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 41..365 e-value: 2.1E-57 score: 193.2 coord: 368..555 e-value: 1.0E-28 score: 98.4
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 55..538 e-value: 1.1E-165 score: 554.3
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 52..549
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 55..330 e-value: 4.9E-31 score: 108.2
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 289..303
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 129..152
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 398..539 e-value: 2.8E-26 score: 92.8
None	No IPR available	GENE3D	3.30.410.40		coord: 176..488 e-value: 1.1E-165 score: 554.3
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 36..553
None	No IPR available	PANTHER	PTHR45968:SF2	(R)-MANDELONITRILE LYASE-LIKE	coord: 36..553
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 326..495

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g000300.1	Lcy05g000300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0046593	mandelonitrile lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors