Lcy04g023730 (gene) Sponge gourd (P93075) v1

BLAST of Lcy04g023730 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-147
Identity = 271/537 (50.47%), Postives = 367/537 (68.34%), Query Frame = 0

Query: 19  ISILLFTTLVISIP--NSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSY 78
           +S+  F   ++S+P  +S   P       + + C S  + S  +   I+   F   + S+
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQA--QITDNVFSRTNPSF 67

Query: 79  SLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYS 138
           S +L A IRN RF + +TPKP  IVTP    HV A++ CS+     ++ RSGGHDYEG S
Sbjct: 68  SSVLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLS 127

Query: 139 YVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVG 198
           Y+S  PFFILDM NLR ++V I +Q+AW+ +GATLGE+YY+I EKS VHGFPAGV PTVG
Sbjct: 128 YISDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVG 187

Query: 199 VGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGI 258
           VGGH+SG GYG+M+RKFG+SVD+++DA IVDVNG++LDR+SMGEDLFWAI GGGGASFG+
Sbjct: 188 VGGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGV 247

Query: 259 IVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSR 318
           ++ +K+KLVPVPETV+VF VE+ M+ GAVD++ KWQ +    D NLF+R++I P  +   
Sbjct: 248 VLGYKVKLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKV 307

Query: 319 PTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD-- 378
            T  A  +ALFLG AE++ AL+ + FPEL LK E+C EM+W +S ++  + +N T  D  
Sbjct: 308 KTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPK 367

Query: 379 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 438
           + L+R+     + K KSDYV   +P+ GIE + KKM+E  +G   ++ NPYGGKM+E+  
Sbjct: 368 VFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTE--LGKIGLVFNPYGGKMAEVTV 427

Query: 439 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 498
           +   FPHR+   +KIQY+VTW +E   +    +L     LY +MT +VSK PR+AYLNYR
Sbjct: 428 NATPFPHRS-KLFKIQYSVTW-QENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYR 487

Query: 499 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSL 552
           D D+GVN  G +   SY +   +GR YF  NFDRLVKVKT  DP NFFR EQSIP++
Sbjct: 488 DVDIGVNDHGTN---SYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Lcy04g023730 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 522.3 bits (1344), Expect = 6.7e-147
Identity = 277/543 (51.01%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 13  TSPTIIISI--LLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFF 72
           T P   +S+    F  L +S+P S      SV  ++ L C S  + S  S   I+   F 
Sbjct: 4   TPPRTFVSVPFFFFFLLFLSLPLSSFSQSNSV-YNSFLKCFSDKTKSPQS--QITDNVFS 63

Query: 73  PDSQSYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGH 132
             + ++S +L A IRN RF + +T KP  I+TP   SHV A++ CS+     ++ RSGGH
Sbjct: 64  QTNPAFSSVLRAYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGH 123

Query: 133 DYEGYSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAG 192
           DY+G SY+S  PFFILDM N+R ++V I   +AW+ +GATLGE+YY+I EKS VHGFPAG
Sbjct: 124 DYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAG 183

Query: 193 VWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGG 252
           V PTVGVGGHLSG GYG+M+RKFG+SVD+V DA IVDVNGRVLDR++MGEDLFWAI GGG
Sbjct: 184 VCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGG 243

Query: 253 GASFGIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFP 312
           G S+G+++ +K+KLVPVP  V+VF VE+ M+ GAVD++ KWQ +    D NLF+R++I P
Sbjct: 244 GGSYGVVLGYKVKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQP 303

Query: 313 DEKGSRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNG 372
             +    T  A  +ALFLG A+++ AL+S+ FPELGLK E+C EM+W +S ++  + LN 
Sbjct: 304 VTRKKVKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNA 363

Query: 373 TSHD--ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGK 432
           T  D  + L+R+     + K KSDYV  A+PK GIE + KKM E  +G   ++ NPYGGK
Sbjct: 364 TQVDPKVFLDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIE--LGKIGLVFNPYGGK 423

Query: 433 MSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRS 492
           M+E+A +   FPHR    +KIQY+V W E   E     YL   + LY +MT +VSK PRS
Sbjct: 424 MAEVAVNAKPFPHR-NKLFKIQYSVNWKENSAE-IEKGYLNQAKVLYSFMTGFVSKNPRS 483

Query: 493 AYLNYRDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSI 552
           +Y NYRD D+GVN  G +   SY +   +GR YF  NFDRLVK+KT VDP NFFR EQSI
Sbjct: 484 SYFNYRDVDIGVNDHGAN---SYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSI 536

BLAST of Lcy04g023730 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.8e-145
Identity = 261/538 (48.51%), Postives = 367/538 (68.22%), Query Frame = 0

Query: 18  IISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYS 77
           +I +L F   +    +S      S +  T   CL+ +S+      PIS   FF  + SYS
Sbjct: 5   LILVLFFVVFIWQSSSS------SANSETFTQCLTSNSDPKH---PISPAIFFSGNGSYS 64

Query: 78  LLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSY 137
            +L A+IRNLRF + +TPKP  I+  TH SHVQA+I C + + LQ++ RSGGHDY+G SY
Sbjct: 65  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 124

Query: 138 VSHS--PFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTV 197
           V++S  PFF+LDMFNLRS+ V +  +TAWV++GA LGE+YY I EKS    +PAG+ PTV
Sbjct: 125 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 184

Query: 198 GVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFG 257
           GVGGH+SG GYG+M+RK+G++VD+ +DA +VDVNG++LDR+ MGEDL+WAI GGGG S+G
Sbjct: 185 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 244

Query: 258 IIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVII--FPDEK 317
           +++++K+ LV VPE V+VF + RT+E+ A DI+ +WQ++A  + + LFIR +I       
Sbjct: 245 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 304

Query: 318 GSRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSH 377
            S+ T    FIA+FLG+   L ++++  FPELGL   DC E SWI+SV+F  +   G+S 
Sbjct: 305 SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSE 364

Query: 378 DILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIA 437
            +LL+R+  P  Y K KSDYV+E + + G+E + KKM E ++ T  M  NPYGG+M  I+
Sbjct: 365 TLLLQRNQ-PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRIS 424

Query: 438 ESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNY 497
            +   FP+RAGN +KIQY   W +   E   D+Y+++ R+LY++MTP+VSK PR ++ NY
Sbjct: 425 STVTPFPYRAGNLWKIQYGANWRD---ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNY 484

Query: 498 RDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSL 552
           RD DLG+N        SY +  ++G+ YF GNF+RLVK+KT VD  NFFR EQSIP L
Sbjct: 485 RDVDLGINSHN-GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Lcy04g023730 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.4e-143
Identity = 261/550 (47.45%), Postives = 374/550 (68.00%), Query Frame = 0

Query: 8   MAFAATSPTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQL 67
           MAFA +      ++ L T L+IS+P S      S  +   + CL  +S+ S    PI+  
Sbjct: 1   MAFAISKRN--ATLFLVTLLLISVPLSS-----STLQQDFVKCLVDNSDVS---FPITAS 60

Query: 68  TFFPDSQS--YSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRT 127
            F PD  +  +   L ++ +NLR+ +P+ PKP FI  P + +HVQA+++C++   L +R 
Sbjct: 61  FFSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRL 120

Query: 128 RSGGHDYEGYSYVS--HSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSN 187
           RSGGHDYEG S+V+   +PF I+D+  LR + V +D  +AW  +GAT+GE+YY+I EKS 
Sbjct: 121 RSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQ 180

Query: 188 VHGFPAGVWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLF 247
            HGFPAG+  ++G+GGHL G  YGSM+RKFG+  D+V+DA IVD NG++LDR +MGED+F
Sbjct: 181 THGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVF 240

Query: 248 WAIRGGGGASFGIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLF 307
           WAIRGGGG SFG+I++WK+KLVPVP TV+VF V +T+E+    +L KW++IAD +D++LF
Sbjct: 241 WAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLF 300

Query: 308 IRVIIFPDEKGSRP---TATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIES 367
           IRVII P  K ++P   T +  + A FLG++ +L  +M +SFPELGL  +DC EMSWI+S
Sbjct: 301 IRVIISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKS 360

Query: 368 VVFAAHFLNGTSHDILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQM 427
           V++ A F N  + + LL    + + ++K KSD+V+E +P  G+EG+ ++  E+   +   
Sbjct: 361 VMYIAGFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLT 420

Query: 428 ILNPYGGKMSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTP 487
           I NPYGG MS I+ESEI FPHR G  +KIQ+  TW  ++G+ + ++++K +RE+Y YM  
Sbjct: 421 IWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTW--QDGKVSEERHMKWIREMYSYMEQ 480

Query: 488 YVSKLPRSAYLNYRDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYN 547
           YVSK PR AY+NYRD DLG        NE    A +WG  Y+KGNF+RLVK+K   DP N
Sbjct: 481 YVSKNPRQAYVNYRDLDLGT-------NEGETDAREWGAKYYKGNFERLVKIKGEFDPDN 529

Query: 548 FFRYEQSIPS 551
           FFR+EQS+P+
Sbjct: 541 FFRHEQSVPT 529

BLAST of Lcy04g023730 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.8e-142
Identity = 263/532 (49.44%), Postives = 354/532 (66.54%), Query Frame = 0

Query: 22  LLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSLLLN 81
           L+FT  ++ IP S     P   + +   C++    S    +PI   T+   + ++  +LN
Sbjct: 9   LIFTVFLLLIPTS--FAAPPKLKDSFTQCVTVFKPS----VPIQNFTYTQQNPNFLTILN 68

Query: 82  ASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYVSHS 141
             +RNLR+ + TT KP  IV   H +H+QA+I C++  GLQ+R RSGGHDY+G SY+S  
Sbjct: 69  NYVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTV 128

Query: 142 PFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKS-NVHGFPAGVWPTVGVGGH 201
            F +LDMFNLR++ +     TAWV+SGATLGE+YY +A KS N+ GFPAG+ P +G GGH
Sbjct: 129 DFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGH 188

Query: 202 LSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIVSW 261
            SG GYG+M+RK+G+S+D+++DA IVD N RVLDR SMGEDLFWA+RGGG ASF ++++W
Sbjct: 189 FSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAW 248

Query: 262 KLKLVPVPETVSVFDVERTMEEGAV--DILDKWQKIADVVDENLFIRVIIFPDEKGSRPT 321
           K+KLVPVPE V+VF+VE     G +  D+  KWQ+IAD +D +LFIR+ +      S  T
Sbjct: 249 KIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTL----SSSNKT 308

Query: 322 ATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTS-HDILL 381
             A F+ ++LGN+EKL  +M+  FPELGL   +C+EM WIESV+F      GT+   ++L
Sbjct: 309 VKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVML 368

Query: 382 ERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEI 441
            R P  + Y K KSDYVQ+ + K G+E + K +SE +     M  NPYGG+MSEI  +E 
Sbjct: 369 NRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENE--NVSMAWNPYGGRMSEIPATET 428

Query: 442 AFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDAD 501
           AFPHRAGN +KIQY+  W    GE+AA   L     ++E M+PYVSK PR A+LNYRD D
Sbjct: 429 AFPHRAGNMFKIQYSSNWF-VPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDID 488

Query: 502 LGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIP 550
           +G N      N +Y +   +G  YFK NF+RLV+VKT VDP N FRYEQSIP
Sbjct: 489 IGKN-----LNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Lcy04g023730 vs. ExPASy TrEMBL
Match: A0A6J1IRG6 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477807 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.7e-215
Identity = 384/549 (69.95%), Postives = 464/549 (84.52%), Query Frame = 0

Query: 15  PTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQ 74
           PT ++ I + TTL++ +P+     + + S HTLL CLS HS+ S+S  PIS++TFFPD+ 
Sbjct: 4   PTSLL-IPILTTLLLILPS-----MAAASHHTLLSCLSDHSSPSAS--PISEVTFFPDNP 63

Query: 75  SYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEG 134
           SYS +LN+ IRNLRFASPTTPKP FIV PTHISH+QASI+C RI+ L++R RSGGHDY+G
Sbjct: 64  SYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDG 123

Query: 135 YSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPT 194
            SYVS SPF ILDMFNLRS+ V I++++AWV+SGATLGE+Y+KIAEKS +HGFPAGV PT
Sbjct: 124 LSYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPT 183

Query: 195 VGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 254
           VGVGGHLSGAGYG+++RKFGVSVD+VVDALIVD NGRVLDRESMGEDLFWAIRGGGGASF
Sbjct: 184 VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASF 243

Query: 255 GIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKG 314
           G+IVSWK KLVP+PETVSVF +E+TMEEGAVD+L KWQ++AD +DENLFIRV+I P  K 
Sbjct: 244 GVIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKK 303

Query: 315 SRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD 374
           ++ TA AKFI+LFLG+A+KL++L+SE+ PELG+KAEDCLEMSWIESV+F +++  GT  +
Sbjct: 304 TQKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLN 363

Query: 375 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 434
           +LLER P  E + K KSDYVQE + KA +EG+++KM E K     +  NPYGGKMSEIAE
Sbjct: 364 VLLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELK--RPALTFNPYGGKMSEIAE 423

Query: 435 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 494
           SE  FPHRAGNKYKIQY+VTW +EEGE+AADK ++++RELYEYMTP+VSK PRSAYLNYR
Sbjct: 424 SETPFPHRAGNKYKIQYSVTW-KEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYR 483

Query: 495 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAF 554
           D DLGVNG   +GN SY +AS+WGR YFKGNFDRLV+VKTMVDP NFFRYEQSIPS  A+
Sbjct: 484 DVDLGVNG---NGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY 538

Query: 555 DSNGRAESL 563
           D NGR ESL
Sbjct: 544 DCNGRGESL 538

BLAST of Lcy04g023730 vs. ExPASy TrEMBL
Match: A0A6J1D0D6 (berberine bridge enzyme-like 21 OS=Momordica charantia OX=3673 GN=LOC111015859 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.7e-215
Identity = 388/548 (70.80%), Postives = 456/548 (83.21%), Query Frame = 0

Query: 21  ILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSLLL 80
           I +F TL+ S+  S PIP    S   LL CLS HS+ SSS  PISQL FFPD+ SYS +L
Sbjct: 9   IPIFLTLLASMAASHPIP----SSQILLQCLSDHSSPSSS--PISQLVFFPDNPSYSPVL 68

Query: 81  NASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYVSH 140
           N+ IRNLRFASPTTP P FIV PTH+SH+QASIIC RI+GL+IR RSGGHDY+G SYVS 
Sbjct: 69  NSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSD 128

Query: 141 SPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVGVGGH 200
           SPF I+DMFNLRS+AV I++++AWVESGATLGE+Y+KIAE+S ++GFPAGV PTVGVGGH
Sbjct: 129 SPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGH 188

Query: 201 LSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIVSW 260
           LSGAGYG+++RKFGVSVD+VVDALIVD NGRVLDRESMGEDLFWAIRGGGGASFGIIVSW
Sbjct: 189 LSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSW 248

Query: 261 KLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSRPTAT 320
           K KLVP+PETV+VF +E+TMEEGAVDIL KWQ+IA  +DENLFIRV+I P  K ++ TA 
Sbjct: 249 KFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAK 308

Query: 321 AKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHDILLERH 380
           AKF++LFLGNAEKLFALMSESFPELG+KAEDC EMSWIESV+F +++ NGTS ++LLER 
Sbjct: 309 AKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLERQ 368

Query: 381 PIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEIAFP 440
           P  E + K KSDYVQE + KA +EG+MKKM E K     +  NPYGGKMS+I ESE  FP
Sbjct: 369 PKSEKFLKKKSDYVQEPISKADLEGMMKKMMELK--RPALTFNPYGGKMSQIPESETPFP 428

Query: 441 HRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDADLGV 500
           HRAGN YKIQY+VTW +EEG++AA++ L+++RELYEYMTP+VSK PRSAYLNYRD DLGV
Sbjct: 429 HRAGNMYKIQYSVTW-KEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGV 488

Query: 501 NGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSLAF------D 560
           NGR  +GN SY +AS WGR YFKGN +RLV+VKT+ DP NFFRYEQSIPS+AF      D
Sbjct: 489 NGR--NGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 545

Query: 561 SNGRAESL 563
           SNG A +L
Sbjct: 549 SNGGAVNL 545

BLAST of Lcy04g023730 vs. ExPASy TrEMBL
Match: A0A6J1ECX0 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433031 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.7e-215
Identity = 385/549 (70.13%), Postives = 463/549 (84.34%), Query Frame = 0

Query: 15  PTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQ 74
           PT ++ I + TTL++ +P+     + + S HTLL CLS HS+ S+S  PIS++TFFPD+ 
Sbjct: 4   PTSLL-IPILTTLLLILPS-----MAAASHHTLLSCLSDHSSPSAS--PISEVTFFPDNP 63

Query: 75  SYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEG 134
           SYS +LN+ IRNLRFASPTTPKP FIV PTHISH+QASI+C RI+ L++R RSGGHDY+G
Sbjct: 64  SYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDG 123

Query: 135 YSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPT 194
            SYVS SPF ILDMFNLRS+AV I++++AWV+SGATLGE+Y+KIAEKS +HGFPAGV PT
Sbjct: 124 LSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPT 183

Query: 195 VGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 254
           VGVGGHLSGAGYG+++RKFGVSVD+VVDALIVD NGR LDRESMGEDLFWAIRGGGGASF
Sbjct: 184 VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASF 243

Query: 255 GIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKG 314
           G+IVSWK +LVP+PETVSVF +E+TMEEGAVD+L KWQ++AD +DENLFIRV+I P  K 
Sbjct: 244 GVIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKK 303

Query: 315 SRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD 374
           ++ TA AKFI+LFLG+A+KLF+LMSE+ PELG+KAEDCLEMSWIESV+F +++  GT  +
Sbjct: 304 TQKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLN 363

Query: 375 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 434
           +LLER P  E + K KSDYVQE + KA +EG+++KM E K     +  NPYGGKMSEIAE
Sbjct: 364 VLLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELK--RPALTFNPYGGKMSEIAE 423

Query: 435 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 494
            E  FPHRAGNKYKIQY+VTW +EEGE+AADK ++++RELYEYMTP+VSK PRSAYLNYR
Sbjct: 424 RETPFPHRAGNKYKIQYSVTW-KEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYR 483

Query: 495 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAF 554
           D DLGVNG   +GN SY +AS WGR YFKGN DRLV+VKTMVDP NFFRYEQSIPS  A+
Sbjct: 484 DVDLGVNG---NGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAY 538

Query: 555 DSNGRAESL 563
           DSNGRAESL
Sbjct: 544 DSNGRAESL 538

BLAST of Lcy04g023730 vs. ExPASy TrEMBL
Match: A0A1S3AZM6 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484295 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 2.3e-214
Identity = 383/547 (70.02%), Postives = 456/547 (83.36%), Query Frame = 0

Query: 20  SILLFT-TLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSL 79
           S+L+ T T ++ I +S  +   + S  TLL CLS HSNS SS  PISQ+TFFPD+ SYS 
Sbjct: 27  SVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLSDHSNSPSSS-PISQVTFFPDNPSYSP 86

Query: 80  LLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYV 139
           +LN+ IRNLRFASPTTPKP FIV PTH+SH+QA+I+C RI+ L+IR RSGGHDY+G SYV
Sbjct: 87  VLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYV 146

Query: 140 SHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVGVG 199
           S SPF ILDMFNLRS+AV I++++AWV+SGATLGE+YYKIAEKS +HGFPAGV PTVGVG
Sbjct: 147 SDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVG 206

Query: 200 GHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIV 259
           GHLSGAGYG+++RKFGVSVD+VVDALIVDVNGRVLDRE+MGEDLFWAIRGGGGASFG+IV
Sbjct: 207 GHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIV 266

Query: 260 SWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSRPT 319
           SWK KLV +PETV+VF  E+T+EEG VDIL KWQ+IAD +DENLFIRV+I P  K ++ T
Sbjct: 267 SWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKT 326

Query: 320 ATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHDILLE 379
           A AKF++LFLGNA+KLFALMSE FPELG+K EDC EMSWI+S++F +++  GT  D+LLE
Sbjct: 327 AKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCKEMSWIDSILFWSNYPIGTPLDVLLE 386

Query: 380 RHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEIA 439
           R P  E + K KSDYVQE + KAG+EG+M+KM E K     +  NPYGGKMS+I E+E  
Sbjct: 387 RQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIELK--RPALTFNPYGGKMSQIPETETP 446

Query: 440 FPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDADL 499
           FPHRAGNKYKIQY+VTW +EEG++AA K+L+++RELY+YMTPYVSK PRSAYLNYRD DL
Sbjct: 447 FPHRAGNKYKIQYSVTW-KEEGDEAAAKHLEMIRELYKYMTPYVSKSPRSAYLNYRDVDL 506

Query: 500 GVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSL---AFDS 559
           GVNG    GN SY + S WGR YFKGNFDRLVKVK+MVDP NFF+YEQSIP +   AFDS
Sbjct: 507 GVNG---IGNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPDNFFKYEQSIPCVELEAFDS 566

Query: 560 NGRAESL 563
           NGRAESL
Sbjct: 567 NGRAESL 566

BLAST of Lcy04g023730 vs. ExPASy TrEMBL
Match: A0A5D3CQQ4 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009800 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 7.3e-213
Identity = 375/523 (71.70%), Postives = 441/523 (84.32%), Query Frame = 0

Query: 43  SRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSLLLNASIRNLRFASPTTPKPCFIVT 102
           S  TLL CLS HSNS SS  PISQ+TFFPD+ SYS +LN+ IRNLRFASPTTPKP FIV 
Sbjct: 9   SHQTLLDCLSDHSNSPSSS-PISQVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVA 68

Query: 103 PTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYVSHSPFFILDMFNLRSLAVYIDEQT 162
           PTH+SH+QA+I+C RI+ L+IR RSGGHDY+G SYVS SPF ILDMFNLRS+AV I +++
Sbjct: 69  PTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIKDES 128

Query: 163 AWVESGATLGELYYKIAEKSNVHGFPAGVWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVD 222
           AWV+SGATLGE+YYKIAEKS +HGFPAGV PTVGVGGHLSGAGYG+++RKFGVSVD+VVD
Sbjct: 129 AWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVD 188

Query: 223 ALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKLKLVPVPETVSVFDVERTMEE 282
           ALIVDVNGRVLDRE+MGEDLFWAIRGGGGASFG+IVSWK KLV +PETV+VF  E+T+EE
Sbjct: 189 ALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFRTEKTIEE 248

Query: 283 GAVDILDKWQKIADVVDENLFIRVIIFPDEKGSRPTATAKFIALFLGNAEKLFALMSESF 342
           G VDIL KWQ+IAD +DENLFIRV+I P  K ++ TA AKF++LFLGNA+KLFALMSE F
Sbjct: 249 GVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLFALMSERF 308

Query: 343 PELGLKAEDCLEMSWIESVVFAAHFLNGTSHDILLERHPIPEGYWKIKSDYVQEAVPKAG 402
           PELG+K EDC EMSWI+S++F +++  GT  D+LLER P  E + K KSDYVQE + KA 
Sbjct: 309 PELGIKGEDCKEMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQEPISKAD 368

Query: 403 IEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEK 462
           +EG+M+KM E K     +  NPYGGKMS+I E+E  FPHRAGNKYKIQY+VTW +EEG++
Sbjct: 369 LEGMMRKMIELK--RPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTW-KEEGDE 428

Query: 463 AADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDADLGVNGRGVSGNESYAKASKWGRSYF 522
           AA K+L+++RELY+YMTPYVSK PRSAYLNYRD DLGVNG    GN SY + S WGR YF
Sbjct: 429 AAAKHLEMIRELYKYMTPYVSKSPRSAYLNYRDVDLGVNG---IGNASYWEGSIWGRKYF 488

Query: 523 KGNFDRLVKVKTMVDPYNFFRYEQSIPSL---AFDSNGRAESL 563
           KGNFDRLVKVK+MVDP NFF+YEQSIP +   AFDSNGRA+SL
Sbjct: 489 KGNFDRLVKVKSMVDPDNFFKYEQSIPCVELEAFDSNGRAKSL 524

BLAST of Lcy04g023730 vs. NCBI nr
Match: XP_023544585.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.2 bits (1959), Expect = 2.5e-215
Identity = 386/549 (70.31%), Postives = 462/549 (84.15%), Query Frame = 0

Query: 15  PTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQ 74
           PT ++ I + TTL++ +P+     + + S HTLL CLS HS+ S+S  PIS++TFFPD+ 
Sbjct: 4   PTSLL-IPILTTLLLILPS-----MAAASHHTLLSCLSDHSSPSAS--PISEVTFFPDNP 63

Query: 75  SYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEG 134
           SYS +LN+ IRNLRFASPTTPKP FIV PTH+SH+QASI+C RI+ L++R RSGGHDY+G
Sbjct: 64  SYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQASILCCRIHALELRIRSGGHDYDG 123

Query: 135 YSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPT 194
            SYVS SPF ILDMFNLRS+AV I++ +AWV+SGATLGE+Y+KIAEKS +HGFPAGV PT
Sbjct: 124 LSYVSDSPFVILDMFNLRSVAVDIEDGSAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPT 183

Query: 195 VGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 254
           VGVGGHLSGAGYG+++RKFGVSVD+VVDALIVD NGRVLDRESMGEDLFWAIRGGGGASF
Sbjct: 184 VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASF 243

Query: 255 GIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKG 314
           G+IVSWK KLVP+PETVSVF +E+TMEEGAVD+L KWQ++AD +DENLFIRV+I P  K 
Sbjct: 244 GVIVSWKFKLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKK 303

Query: 315 SRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD 374
           ++ TA AKFI+LFLG+A+KLF+LMSE+ PELG+KAEDCLEMSWIESV+F +++  GT  +
Sbjct: 304 TQKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPTGTPLN 363

Query: 375 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 434
           +LLER P  E + K KSDYVQE + KA +EG+M+KM E K     +  NPYGGKMSEIAE
Sbjct: 364 VLLERQPNSEKFLKKKSDYVQEPISKADLEGMMRKMMELK--RPALTFNPYGGKMSEIAE 423

Query: 435 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 494
           SE  FPHRAGN+YKIQY+VTW +EEGE+AADK + ++RELYEYMTP+VSK PRSAYLNYR
Sbjct: 424 SETPFPHRAGNRYKIQYSVTW-KEEGEEAADKNVGLIRELYEYMTPHVSKSPRSAYLNYR 483

Query: 495 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAF 554
           D DLGVNG   +GN SY +AS WGR YFKGNFDRLV+VKTMVDP NFFRYEQSIPS  A+
Sbjct: 484 DVDLGVNG---NGNASYWEASHWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY 538

Query: 555 DSNGRAESL 563
           D NGRAE L
Sbjct: 544 DCNGRAERL 538

BLAST of Lcy04g023730 vs. NCBI nr
Match: XP_022977474.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 758.1 bits (1956), Expect = 5.5e-215
Identity = 384/549 (69.95%), Postives = 464/549 (84.52%), Query Frame = 0

Query: 15  PTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQ 74
           PT ++ I + TTL++ +P+     + + S HTLL CLS HS+ S+S  PIS++TFFPD+ 
Sbjct: 4   PTSLL-IPILTTLLLILPS-----MAAASHHTLLSCLSDHSSPSAS--PISEVTFFPDNP 63

Query: 75  SYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEG 134
           SYS +LN+ IRNLRFASPTTPKP FIV PTHISH+QASI+C RI+ L++R RSGGHDY+G
Sbjct: 64  SYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDG 123

Query: 135 YSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPT 194
            SYVS SPF ILDMFNLRS+ V I++++AWV+SGATLGE+Y+KIAEKS +HGFPAGV PT
Sbjct: 124 LSYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPT 183

Query: 195 VGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 254
           VGVGGHLSGAGYG+++RKFGVSVD+VVDALIVD NGRVLDRESMGEDLFWAIRGGGGASF
Sbjct: 184 VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASF 243

Query: 255 GIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKG 314
           G+IVSWK KLVP+PETVSVF +E+TMEEGAVD+L KWQ++AD +DENLFIRV+I P  K 
Sbjct: 244 GVIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKK 303

Query: 315 SRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD 374
           ++ TA AKFI+LFLG+A+KL++L+SE+ PELG+KAEDCLEMSWIESV+F +++  GT  +
Sbjct: 304 TQKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLN 363

Query: 375 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 434
           +LLER P  E + K KSDYVQE + KA +EG+++KM E K     +  NPYGGKMSEIAE
Sbjct: 364 VLLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELK--RPALTFNPYGGKMSEIAE 423

Query: 435 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 494
           SE  FPHRAGNKYKIQY+VTW +EEGE+AADK ++++RELYEYMTP+VSK PRSAYLNYR
Sbjct: 424 SETPFPHRAGNKYKIQYSVTW-KEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYR 483

Query: 495 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAF 554
           D DLGVNG   +GN SY +AS+WGR YFKGNFDRLV+VKTMVDP NFFRYEQSIPS  A+
Sbjct: 484 DVDLGVNG---NGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAY 538

Query: 555 DSNGRAESL 563
           D NGR ESL
Sbjct: 544 DCNGRGESL 538

BLAST of Lcy04g023730 vs. NCBI nr
Match: XP_022925684.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 758.1 bits (1956), Expect = 5.5e-215
Identity = 385/549 (70.13%), Postives = 463/549 (84.34%), Query Frame = 0

Query: 15  PTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQ 74
           PT ++ I + TTL++ +P+     + + S HTLL CLS HS+ S+S  PIS++TFFPD+ 
Sbjct: 4   PTSLL-IPILTTLLLILPS-----MAAASHHTLLSCLSDHSSPSAS--PISEVTFFPDNP 63

Query: 75  SYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEG 134
           SYS +LN+ IRNLRFASPTTPKP FIV PTHISH+QASI+C RI+ L++R RSGGHDY+G
Sbjct: 64  SYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDG 123

Query: 135 YSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPT 194
            SYVS SPF ILDMFNLRS+AV I++++AWV+SGATLGE+Y+KIAEKS +HGFPAGV PT
Sbjct: 124 LSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPT 183

Query: 195 VGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 254
           VGVGGHLSGAGYG+++RKFGVSVD+VVDALIVD NGR LDRESMGEDLFWAIRGGGGASF
Sbjct: 184 VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASF 243

Query: 255 GIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKG 314
           G+IVSWK +LVP+PETVSVF +E+TMEEGAVD+L KWQ++AD +DENLFIRV+I P  K 
Sbjct: 244 GVIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKK 303

Query: 315 SRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD 374
           ++ TA AKFI+LFLG+A+KLF+LMSE+ PELG+KAEDCLEMSWIESV+F +++  GT  +
Sbjct: 304 TQKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLN 363

Query: 375 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 434
           +LLER P  E + K KSDYVQE + KA +EG+++KM E K     +  NPYGGKMSEIAE
Sbjct: 364 VLLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELK--RPALTFNPYGGKMSEIAE 423

Query: 435 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 494
            E  FPHRAGNKYKIQY+VTW +EEGE+AADK ++++RELYEYMTP+VSK PRSAYLNYR
Sbjct: 424 RETPFPHRAGNKYKIQYSVTW-KEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYR 483

Query: 495 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAF 554
           D DLGVNG   +GN SY +AS WGR YFKGN DRLV+VKTMVDP NFFRYEQSIPS  A+
Sbjct: 484 DVDLGVNG---NGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAY 538

Query: 555 DSNGRAESL 563
           DSNGRAESL
Sbjct: 544 DSNGRAESL 538

BLAST of Lcy04g023730 vs. NCBI nr
Match: XP_022146727.1 (berberine bridge enzyme-like 21 [Momordica charantia])

HSP 1 Score: 758.1 bits (1956), Expect = 5.5e-215
Identity = 388/548 (70.80%), Postives = 456/548 (83.21%), Query Frame = 0

Query: 21  ILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSLLL 80
           I +F TL+ S+  S PIP    S   LL CLS HS+ SSS  PISQL FFPD+ SYS +L
Sbjct: 9   IPIFLTLLASMAASHPIP----SSQILLQCLSDHSSPSSS--PISQLVFFPDNPSYSPVL 68

Query: 81  NASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYVSH 140
           N+ IRNLRFASPTTP P FIV PTH+SH+QASIIC RI+GL+IR RSGGHDY+G SYVS 
Sbjct: 69  NSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGLSYVSD 128

Query: 141 SPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVGVGGH 200
           SPF I+DMFNLRS+AV I++++AWVESGATLGE+Y+KIAE+S ++GFPAGV PTVGVGGH
Sbjct: 129 SPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTVGVGGH 188

Query: 201 LSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIVSW 260
           LSGAGYG+++RKFGVSVD+VVDALIVD NGRVLDRESMGEDLFWAIRGGGGASFGIIVSW
Sbjct: 189 LSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFGIIVSW 248

Query: 261 KLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSRPTAT 320
           K KLVP+PETV+VF +E+TMEEGAVDIL KWQ+IA  +DENLFIRV+I P  K ++ TA 
Sbjct: 249 KFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKAKSTAK 308

Query: 321 AKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHDILLERH 380
           AKF++LFLGNAEKLFALMSESFPELG+KAEDC EMSWIESV+F +++ NGTS ++LLER 
Sbjct: 309 AKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNVLLERQ 368

Query: 381 PIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEIAFP 440
           P  E + K KSDYVQE + KA +EG+MKKM E K     +  NPYGGKMS+I ESE  FP
Sbjct: 369 PKSEKFLKKKSDYVQEPISKADLEGMMKKMMELK--RPALTFNPYGGKMSQIPESETPFP 428

Query: 441 HRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDADLGV 500
           HRAGN YKIQY+VTW +EEG++AA++ L+++RELYEYMTP+VSK PRSAYLNYRD DLGV
Sbjct: 429 HRAGNMYKIQYSVTW-KEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDLGV 488

Query: 501 NGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSLAF------D 560
           NGR  +GN SY +AS WGR YFKGN +RLV+VKT+ DP NFFRYEQSIPS+AF      D
Sbjct: 489 NGR--NGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 545

Query: 561 SNGRAESL 563
           SNG A +L
Sbjct: 549 SNGGAVNL 545

BLAST of Lcy04g023730 vs. NCBI nr
Match: KAG6581486.1 (Berberine bridge enzyme-like 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.9 bits (1953), Expect = 1.2e-214
Identity = 378/523 (72.28%), Postives = 448/523 (85.66%), Query Frame = 0

Query: 41  SVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYSLLLNASIRNLRFASPTTPKPCFI 100
           + S HTLL CLS HS+ S+S  PIS++TFFPD+ SYS +LN+ IRNLRFASPTTPKP FI
Sbjct: 3   AASHHTLLSCLSDHSSPSAS--PISEVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFI 62

Query: 101 VTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSYVSHSPFFILDMFNLRSLAVYIDE 160
           V PTH+SH+QASI+C RI+ L++R RSGGHDY+G SYVS SPF ILDMFNLRS+AV I++
Sbjct: 63  VAPTHVSHIQASILCCRIHALELRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIED 122

Query: 161 QTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVGVGGHLSGAGYGSMIRKFGVSVDHV 220
           ++AWV+SGATLGE+Y+KIAEKS +HGFPAGV PTVGVGGHLSGAGYG+++RKFGVSVD+V
Sbjct: 123 ESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNV 182

Query: 221 VDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGIIVSWKLKLVPVPETVSVFDVERTM 280
           VDALIVD +GR LDRESMGEDLFWAIRGGGGASFG+IVSWK KLVP+PETVSVF +E+TM
Sbjct: 183 VDALIVDSSGRALDRESMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVSVFRMEKTM 242

Query: 281 EEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSRPTATAKFIALFLGNAEKLFALMSE 340
           EEGAVD+L KWQ++AD +DENLFIRV+I P  K ++ TA AKFI+LFLG+A+KLF+ MSE
Sbjct: 243 EEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKTQKTAKAKFISLFLGSAQKLFSSMSE 302

Query: 341 SFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHDILLERHPIPEGYWKIKSDYVQEAVPK 400
           + PELG+KAEDCLEMSWIESV+F +++  GT  ++LLER P  E + K KSDYVQE + K
Sbjct: 303 TLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNVLLERQPNSEKFLKKKSDYVQEPISK 362

Query: 401 AGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAESEIAFPHRAGNKYKIQYAVTWAEEEG 460
           A +EG+++KM E K     +  NPYGGKMSEIAESE  FPHRAGNKYKIQY+VTW +EEG
Sbjct: 363 ADLEGMLRKMMELK--RPALTFNPYGGKMSEIAESETPFPHRAGNKYKIQYSVTW-KEEG 422

Query: 461 EKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYRDADLGVNGRGVSGNESYAKASKWGRS 520
           E+AADK + ++RELYEYMTP+VSK PRSAYLNYRD DLGVNG   +GN SY +AS WGR 
Sbjct: 423 EEAADKNVGLIRELYEYMTPHVSKSPRSAYLNYRDVDLGVNG---NGNASYWEASHWGRK 482

Query: 521 YFKGNFDRLVKVKTMVDPYNFFRYEQSIPS-LAFDSNGRAESL 563
           YFKGNFDRLV+VKTMVDP NFFRYEQSIPS  A+DSNGRAESL
Sbjct: 483 YFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAYDSNGRAESL 517

BLAST of Lcy04g023730 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 9.6e-149
Identity = 271/537 (50.47%), Postives = 367/537 (68.34%), Query Frame = 0

Query: 19  ISILLFTTLVISIP--NSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSY 78
           +S+  F   ++S+P  +S   P       + + C S  + S  +   I+   F   + S+
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQA--QITDNVFSRTNPSF 67

Query: 79  SLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYS 138
           S +L A IRN RF + +TPKP  IVTP    HV A++ CS+     ++ RSGGHDYEG S
Sbjct: 68  SSVLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLS 127

Query: 139 YVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTVG 198
           Y+S  PFFILDM NLR ++V I +Q+AW+ +GATLGE+YY+I EKS VHGFPAGV PTVG
Sbjct: 128 YISDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVG 187

Query: 199 VGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGI 258
           VGGH+SG GYG+M+RKFG+SVD+++DA IVDVNG++LDR+SMGEDLFWAI GGGGASFG+
Sbjct: 188 VGGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGV 247

Query: 259 IVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFPDEKGSR 318
           ++ +K+KLVPVPETV+VF VE+ M+ GAVD++ KWQ +    D NLF+R++I P  +   
Sbjct: 248 VLGYKVKLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKV 307

Query: 319 PTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSHD-- 378
            T  A  +ALFLG AE++ AL+ + FPEL LK E+C EM+W +S ++  + +N T  D  
Sbjct: 308 KTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPK 367

Query: 379 ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIAE 438
           + L+R+     + K KSDYV   +P+ GIE + KKM+E  +G   ++ NPYGGKM+E+  
Sbjct: 368 VFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTE--LGKIGLVFNPYGGKMAEVTV 427

Query: 439 SEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNYR 498
           +   FPHR+   +KIQY+VTW +E   +    +L     LY +MT +VSK PR+AYLNYR
Sbjct: 428 NATPFPHRS-KLFKIQYSVTW-QENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYR 487

Query: 499 DADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSL 552
           D D+GVN  G +   SY +   +GR YF  NFDRLVKVKT  DP NFFR EQSIP++
Sbjct: 488 DVDIGVNDHGTN---SYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Lcy04g023730 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 522.3 bits (1344), Expect = 4.8e-148
Identity = 277/543 (51.01%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 13  TSPTIIISI--LLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFF 72
           T P   +S+    F  L +S+P S      SV  ++ L C S  + S  S   I+   F 
Sbjct: 4   TPPRTFVSVPFFFFFLLFLSLPLSSFSQSNSV-YNSFLKCFSDKTKSPQS--QITDNVFS 63

Query: 73  PDSQSYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGH 132
             + ++S +L A IRN RF + +T KP  I+TP   SHV A++ CS+     ++ RSGGH
Sbjct: 64  QTNPAFSSVLRAYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGH 123

Query: 133 DYEGYSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAG 192
           DY+G SY+S  PFFILDM N+R ++V I   +AW+ +GATLGE+YY+I EKS VHGFPAG
Sbjct: 124 DYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAG 183

Query: 193 VWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGG 252
           V PTVGVGGHLSG GYG+M+RKFG+SVD+V DA IVDVNGRVLDR++MGEDLFWAI GGG
Sbjct: 184 VCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGG 243

Query: 253 GASFGIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFP 312
           G S+G+++ +K+KLVPVP  V+VF VE+ M+ GAVD++ KWQ +    D NLF+R++I P
Sbjct: 244 GGSYGVVLGYKVKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQP 303

Query: 313 DEKGSRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNG 372
             +    T  A  +ALFLG A+++ AL+S+ FPELGLK E+C EM+W +S ++  + LN 
Sbjct: 304 VTRKKVKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNA 363

Query: 373 TSHD--ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGK 432
           T  D  + L+R+     + K KSDYV  A+PK GIE + KKM E  +G   ++ NPYGGK
Sbjct: 364 TQVDPKVFLDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIE--LGKIGLVFNPYGGK 423

Query: 433 MSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRS 492
           M+E+A +   FPHR    +KIQY+V W E   E     YL   + LY +MT +VSK PRS
Sbjct: 424 MAEVAVNAKPFPHR-NKLFKIQYSVNWKENSAE-IEKGYLNQAKVLYSFMTGFVSKNPRS 483

Query: 493 AYLNYRDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSI 552
           +Y NYRD D+GVN  G +   SY +   +GR YF  NFDRLVK+KT VDP NFFR EQSI
Sbjct: 484 SYFNYRDVDIGVNDHGAN---SYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSI 536

BLAST of Lcy04g023730 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 522.3 bits (1344), Expect = 4.8e-148
Identity = 277/543 (51.01%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 13  TSPTIIISI--LLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFF 72
           T P   +S+    F  L +S+P S      SV  ++ L C S  + S  S   I+   F 
Sbjct: 4   TPPRTFVSVPFFFFFLLFLSLPLSSFSQSNSV-YNSFLKCFSDKTKSPQS--QITDNVFS 63

Query: 73  PDSQSYSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGH 132
             + ++S +L A IRN RF + +T KP  I+TP   SHV A++ CS+     ++ RSGGH
Sbjct: 64  QTNPAFSSVLRAYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGH 123

Query: 133 DYEGYSYVSHSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAG 192
           DY+G SY+S  PFFILDM N+R ++V I   +AW+ +GATLGE+YY+I EKS VHGFPAG
Sbjct: 124 DYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAG 183

Query: 193 VWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGG 252
           V PTVGVGGHLSG GYG+M+RKFG+SVD+V DA IVDVNGRVLDR++MGEDLFWAI GGG
Sbjct: 184 VCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGG 243

Query: 253 GASFGIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVIIFP 312
           G S+G+++ +K+KLVPVP  V+VF VE+ M+ GAVD++ KWQ +    D NLF+R++I P
Sbjct: 244 GGSYGVVLGYKVKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQP 303

Query: 313 DEKGSRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNG 372
             +    T  A  +ALFLG A+++ AL+S+ FPELGLK E+C EM+W +S ++  + LN 
Sbjct: 304 VTRKKVKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNA 363

Query: 373 TSHD--ILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGK 432
           T  D  + L+R+     + K KSDYV  A+PK GIE + KKM E  +G   ++ NPYGGK
Sbjct: 364 TQVDPKVFLDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIE--LGKIGLVFNPYGGK 423

Query: 433 MSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRS 492
           M+E+A +   FPHR    +KIQY+V W E   E     YL   + LY +MT +VSK PRS
Sbjct: 424 MAEVAVNAKPFPHR-NKLFKIQYSVNWKENSAE-IEKGYLNQAKVLYSFMTGFVSKNPRS 483

Query: 493 AYLNYRDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSI 552
           +Y NYRD D+GVN  G +   SY +   +GR YF  NFDRLVK+KT VDP NFFR EQSI
Sbjct: 484 SYFNYRDVDIGVNDHGAN---SYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSI 536

BLAST of Lcy04g023730 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 2.0e-146
Identity = 261/538 (48.51%), Postives = 367/538 (68.22%), Query Frame = 0

Query: 18  IISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQLTFFPDSQSYS 77
           +I +L F   +    +S      S +  T   CL+ +S+      PIS   FF  + SYS
Sbjct: 5   LILVLFFVVFIWQSSSS------SANSETFTQCLTSNSDPKH---PISPAIFFSGNGSYS 64

Query: 78  LLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRTRSGGHDYEGYSY 137
            +L A+IRNLRF + +TPKP  I+  TH SHVQA+I C + + LQ++ RSGGHDY+G SY
Sbjct: 65  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 124

Query: 138 VSHS--PFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSNVHGFPAGVWPTV 197
           V++S  PFF+LDMFNLRS+ V +  +TAWV++GA LGE+YY I EKS    +PAG+ PTV
Sbjct: 125 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 184

Query: 198 GVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFG 257
           GVGGH+SG GYG+M+RK+G++VD+ +DA +VDVNG++LDR+ MGEDL+WAI GGGG S+G
Sbjct: 185 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 244

Query: 258 IIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLFIRVII--FPDEK 317
           +++++K+ LV VPE V+VF + RT+E+ A DI+ +WQ++A  + + LFIR +I       
Sbjct: 245 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 304

Query: 318 GSRPTATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIESVVFAAHFLNGTSH 377
            S+ T    FIA+FLG+   L ++++  FPELGL   DC E SWI+SV+F  +   G+S 
Sbjct: 305 SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSE 364

Query: 378 DILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQMILNPYGGKMSEIA 437
            +LL+R+  P  Y K KSDYV+E + + G+E + KKM E ++ T  M  NPYGG+M  I+
Sbjct: 365 TLLLQRNQ-PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRIS 424

Query: 438 ESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTPYVSKLPRSAYLNY 497
            +   FP+RAGN +KIQY   W +   E   D+Y+++ R+LY++MTP+VSK PR ++ NY
Sbjct: 425 STVTPFPYRAGNLWKIQYGANWRD---ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNY 484

Query: 498 RDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYNFFRYEQSIPSL 552
           RD DLG+N        SY +  ++G+ YF GNF+RLVK+KT VD  NFFR EQSIP L
Sbjct: 485 RDVDLGINSHN-GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Lcy04g023730 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.4e-144
Identity = 261/550 (47.45%), Postives = 374/550 (68.00%), Query Frame = 0

Query: 8   MAFAATSPTIIISILLFTTLVISIPNSDPIPIPSVSRHTLLHCLSQHSNSSSSPIPISQL 67
           MAFA +      ++ L T L+IS+P S      S  +   + CL  +S+ S    PI+  
Sbjct: 1   MAFAISKRN--ATLFLVTLLLISVPLSS-----STLQQDFVKCLVDNSDVS---FPITAS 60

Query: 68  TFFPDSQS--YSLLLNASIRNLRFASPTTPKPCFIVTPTHISHVQASIICSRIYGLQIRT 127
            F PD  +  +   L ++ +NLR+ +P+ PKP FI  P + +HVQA+++C++   L +R 
Sbjct: 61  FFSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRL 120

Query: 128 RSGGHDYEGYSYVS--HSPFFILDMFNLRSLAVYIDEQTAWVESGATLGELYYKIAEKSN 187
           RSGGHDYEG S+V+   +PF I+D+  LR + V +D  +AW  +GAT+GE+YY+I EKS 
Sbjct: 121 RSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQ 180

Query: 188 VHGFPAGVWPTVGVGGHLSGAGYGSMIRKFGVSVDHVVDALIVDVNGRVLDRESMGEDLF 247
            HGFPAG+  ++G+GGHL G  YGSM+RKFG+  D+V+DA IVD NG++LDR +MGED+F
Sbjct: 181 THGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVF 240

Query: 248 WAIRGGGGASFGIIVSWKLKLVPVPETVSVFDVERTMEEGAVDILDKWQKIADVVDENLF 307
           WAIRGGGG SFG+I++WK+KLVPVP TV+VF V +T+E+    +L KW++IAD +D++LF
Sbjct: 241 WAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLF 300

Query: 308 IRVIIFPDEKGSRP---TATAKFIALFLGNAEKLFALMSESFPELGLKAEDCLEMSWIES 367
           IRVII P  K ++P   T +  + A FLG++ +L  +M +SFPELGL  +DC EMSWI+S
Sbjct: 301 IRVIISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKS 360

Query: 368 VVFAAHFLNGTSHDILLERHPIPEGYWKIKSDYVQEAVPKAGIEGVMKKMSEQKVGTAQM 427
           V++ A F N  + + LL    + + ++K KSD+V+E +P  G+EG+ ++  E+   +   
Sbjct: 361 VMYIAGFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLT 420

Query: 428 ILNPYGGKMSEIAESEIAFPHRAGNKYKIQYAVTWAEEEGEKAADKYLKVVRELYEYMTP 487
           I NPYGG MS I+ESEI FPHR G  +KIQ+  TW  ++G+ + ++++K +RE+Y YM  
Sbjct: 421 IWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTW--QDGKVSEERHMKWIREMYSYMEQ 480

Query: 488 YVSKLPRSAYLNYRDADLGVNGRGVSGNESYAKASKWGRSYFKGNFDRLVKVKTMVDPYN 547
           YVSK PR AY+NYRD DLG        NE    A +WG  Y+KGNF+RLVK+K   DP N
Sbjct: 481 YVSKNPRQAYVNYRDLDLGT-------NEGETDAREWGAKYYKGNFERLVKIKGEFDPDN 529

Query: 548 FFRYEQSIPS 551
           FFR+EQS+P+
Sbjct: 541 FFRHEQSVPT 529