Lcy04g022950 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g022950
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationChr04: 54051198 .. 54054190 (-)
RNA-Seq ExpressionLcy04g022950
SyntenyLcy04g022950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAATCTTCTACACAAAACATTCCCACCAAAGCTTTTCAAAACATACCAACATAATTTTCTTAGCTAATTTCTCAATCTCTCCTCTCTCATTGCCATGATCTATCTTTTTCTTCTATTTTCTCTCACATTTTCAGGTATATGTGGAGATTCAACAAATCTTCAAGTGATTTTCTGTTTTTTAATGTCATTTTTGTTTATTGTGTGTTCTTACCTAGCTTGATTTTCTTTTTTTTTTTTTTTTGATATATAGGAGTTGAATCTCTAGGTATTAATTATGGACAAGTTGGTGATAATTTGCCTCCACCAGATAAGGTTCTCGAGCTGTTAACTTCTCTTAAGATCTCAAAAGTAAGAATTTACGATACAAATCCTCAAATATTGACAACATTTGCCAACTCCAAGACCGAGCTCATCGTGACCATAGAAAACGAGATGCTAAGCCAACTTATGGACCCTCAACAAGCCGTCCAATGGGTTGCAACCCACATCAAGCCCTACTTCCCGGCGGCCAGGATCACCGGGATCGCGGTCGGGAACGAGATATTCACTGGAAACGACACGACCCTGATGGCGAACCTGGTCCCGGCGATGATCAGCATCCATGGTGCTCTGGGTCAGCTAGGGCTAGGGTCATACATTCATGTGTCAACTCCAACATCCTTGGCTGTTCTTGAGGAATCCTTCCCTCCTTCAGCTGGTAGCTTCAAATCCCAAGTCTCAACCTACATTTCACAGTTCTTGCAGTTCTTATCAAGCACAAAGGCTCCATTTTGGATCAATGCATACCCTTATTTTGCATACAAGGACAACCCCAATATAATATCTTTGGATTATGCTCTGTTAAATCCCATGTCTTACATGGTTGACCCTTCCACAAAGCTGAATTATGACAACATGTTGTATGCTCAAGTGGATGCAGTGATATTTGCAATGGCAAGGTTGGGTTTTGGTGGGATTGAGGTTAGGGTTTCAGAGACAGGGTGGCCTTCAAAAGGAGACATTGAGGAGATTGGAGCAACAGCTGAGAATGCAGCCATTTACAATAGGAATTTGTTGAGAAGACAATTGGCTAATGAAGGAACTCCTCTAAGGCCAAATATGAGACTTGAAGTTTATCTTTTTGCTCTCTTTAATGAAGACATGAAGCCTGGACCAACCTCTGAAAGAAACTATGGTCTCTATCAACCTGATGGAACCATGGCTTACAATGTTGGCCTTTCCGCCTTGTTGACTTCTTCTTCAACTTCTTCTGCATCCATCTCCCTATCTTCTTCGGCCTCAAAGGTAAAAACTTTTCGTTTCCTTAAAATACATTCATTAAACAAGTTAATTACAGTATTATCATAACAAATTTTGCTTTTGATTTCACCTAAAACGTCTTATATCAGTGGAAAATTTGATATTGTTCGTATTCTTAACGACCCGTTAAAAAAAGAAGAAAGCAAAGAACTTTTTCTTTTGTAATGGTCATCAGAAAATTTCTAACTTTATTGTGTTTTTTTTTTTCCTCTTTGACAGACAAGAACAATGGAATATAAAAGCTTAGTGTATTGGATGTTTGTGTATTTGCTGCTGAGCTTCCAGATTATTCCGACGAGAAGACCATTTTAGAAGAAAATTATTGGTGGGTTAAGGGTTTGATTATATCATTTAATTAAGCAAATTAATTAAAGCCCATCAACCATTTGATTAACTTTAGTTTGTTAGTTTTGATTTGGTGTAAAATTCACTAAAAATGGCCTTGATTTGATTCTTCTGTAGCCATTAATTAAATTATTGTTCTTCATGTATAGCTCTCTCTATTTCATGTACGTAAAACCAAAAAGGCCTTGTAATTCCATGCCATTCGTTTAATGTTATATTATATTCTTAATGTCTCTAATTCTATATTAATTAATTAATTAATGTTAATTAATGTCTCTGGCTGAAATTAATTAGTAATATTACAACCTTGTTTTGCTCACATCTTTTGTCGTAAAGAAAGAAAGAATATATAATAATATGGAAAGATAAGAAAAGAAATTGTCCTCTCTCATCTGTGGGACGTGGAATCCACATGCAACTGTTTCTATTTCAAATTGAAAAGTCACTTTTTTTTTTTTTTTTAGTTTTATGCTTTTTCTTTTTCTACGAGCAGCTAAAATATGGCTGTCTTCTAATAAGTAATAACCCTTGGATGCTGGTTCAAAATTTGTATTCTGTTCTTCAATTATCTCAAAAATATCTAATAAGGTAGAATCGCTTGCTACATTTGTTCCATTACCTTAAATAATTTCTCGAGATAGAGTGCGAAGACTATATAAGGCATTAATGAGAAAAATAAAATGAAAAATGAAACACTTTAAAGTGTAAAGTAAAAAACTTTATTTTGTGACTTTTTATTATCCTGAGCAGACAAATGGTCGGCCAAGCTTTATTCCTTTGGCAAACCCTAACCATCAGCAGAGCTTCAATTTGGGCATCTTTTTTCTTCTCTACAAATATAAAAACCTCTCCTTTATTTACTGATTGGACCAAAAAAAACACAGAAAAAAAAAAAAAAAAAAAATCCATTTTTACATATTGTTTCAATTATTTTTGTTCGATTTTGTGTTTTTTTATATAATATAATATGAACCCATTTTTCCTTTTTTGCTGATGAAAAAGAAATCAGCTGCTTCAATATTATTCAAAAAAGGGATAAACTTGTGAGAGACTTTTTAAAGTTTTAATTTTATTTGAATAATGTTGGAATTTCAGGTAGCTGATGAGTCATCATAGTGGATTACTGCGTCAGATTGGAGGGTCCTCATTTTCTACACAAATCAGCATGTGCTTCTGTACGTTCTAGAGAGAGACAAGAGAGAGAAAGAGATATAGTGAGTGCCAATTACAGTCATTGCCATTGCATACAACCTTTTCATCTAAGTATATCATCTATCTCGACGAATATCAGTCATATTGTGACGGTCTCTTTTGTTTAAAATATATATAAAAGATTAAACAATATGT

mRNA sequence

GCAAATCTTCTACACAAAACATTCCCACCAAAGCTTTTCAAAACATACCAACATAATTTTCTTAGCTAATTTCTCAATCTCTCCTCTCTCATTGCCATGATCTATCTTTTTCTTCTATTTTCTCTCACATTTTCAGGAGTTGAATCTCTAGGTATTAATTATGGACAAGTTGGTGATAATTTGCCTCCACCAGATAAGGTTCTCGAGCTGTTAACTTCTCTTAAGATCTCAAAAGTAAGAATTTACGATACAAATCCTCAAATATTGACAACATTTGCCAACTCCAAGACCGAGCTCATCGTGACCATAGAAAACGAGATGCTAAGCCAACTTATGGACCCTCAACAAGCCGTCCAATGGGTTGCAACCCACATCAAGCCCTACTTCCCGGCGGCCAGGATCACCGGGATCGCGGTCGGGAACGAGATATTCACTGGAAACGACACGACCCTGATGGCGAACCTGGTCCCGGCGATGATCAGCATCCATGGTGCTCTGGGTCAGCTAGGGCTAGGGTCATACATTCATGTGTCAACTCCAACATCCTTGGCTGTTCTTGAGGAATCCTTCCCTCCTTCAGCTGGTAGCTTCAAATCCCAAGTCTCAACCTACATTTCACAGTTCTTGCAGTTCTTATCAAGCACAAAGGCTCCATTTTGGATCAATGCATACCCTTATTTTGCATACAAGGACAACCCCAATATAATATCTTTGGATTATGCTCTGTTAAATCCCATGTCTTACATGGTTGACCCTTCCACAAAGCTGAATTATGACAACATGTTGTATGCTCAAGTGGATGCAGTGATATTTGCAATGGCAAGGTTGGGTTTTGGTGGGATTGAGGTTAGGGTTTCAGAGACAGGGTGGCCTTCAAAAGGAGACATTGAGGAGATTGGAGCAACAGCTGAGAATGCAGCCATTTACAATAGGAATTTGTTGAGAAGACAATTGGCTAATGAAGGAACTCCTCTAAGGCCAAATATGAGACTTGAAGTTTATCTTTTTGCTCTCTTTAATGAAGACATGAAGCCTGGACCAACCTCTGAAAGAAACTATGGTCTCTATCAACCTGATGGAACCATGGCTTACAATGTTGGCCTTTCCGCCTTGTTGACTTCTTCTTCAACTTCTTCTGCATCCATCTCCCTATCTTCTTCGGCCTCAAAGACAAGAACAATGGAATATAAAAGCTTAGTGTATTGGATGTTTGTGTATTTGCTGCTGAGCTTCCAGATTATTCCGACGAGAAGACCATTTTAGAAGAAAATTATTGGTAGCTGATGAGTCATCATAGTGGATTACTGCGTCAGATTGGAGGGTCCTCATTTTCTACACAAATCAGCATGTGCTTCTGTACGTTCTAGAGAGAGACAAGAGAGAGAAAGAGATATAGTGAGTGCCAATTACAGTCATTGCCATTGCATACAACCTTTTCATCTAAGTATATCATCTATCTCGACGAATATCAGTCATATTGTGACGGTCTCTTTTGTTTAAAATATATATAAAAGATTAAACAATATGT

Coding sequence (CDS)

ATGATCTATCTTTTTCTTCTATTTTCTCTCACATTTTCAGGAGTTGAATCTCTAGGTATTAATTATGGACAAGTTGGTGATAATTTGCCTCCACCAGATAAGGTTCTCGAGCTGTTAACTTCTCTTAAGATCTCAAAAGTAAGAATTTACGATACAAATCCTCAAATATTGACAACATTTGCCAACTCCAAGACCGAGCTCATCGTGACCATAGAAAACGAGATGCTAAGCCAACTTATGGACCCTCAACAAGCCGTCCAATGGGTTGCAACCCACATCAAGCCCTACTTCCCGGCGGCCAGGATCACCGGGATCGCGGTCGGGAACGAGATATTCACTGGAAACGACACGACCCTGATGGCGAACCTGGTCCCGGCGATGATCAGCATCCATGGTGCTCTGGGTCAGCTAGGGCTAGGGTCATACATTCATGTGTCAACTCCAACATCCTTGGCTGTTCTTGAGGAATCCTTCCCTCCTTCAGCTGGTAGCTTCAAATCCCAAGTCTCAACCTACATTTCACAGTTCTTGCAGTTCTTATCAAGCACAAAGGCTCCATTTTGGATCAATGCATACCCTTATTTTGCATACAAGGACAACCCCAATATAATATCTTTGGATTATGCTCTGTTAAATCCCATGTCTTACATGGTTGACCCTTCCACAAAGCTGAATTATGACAACATGTTGTATGCTCAAGTGGATGCAGTGATATTTGCAATGGCAAGGTTGGGTTTTGGTGGGATTGAGGTTAGGGTTTCAGAGACAGGGTGGCCTTCAAAAGGAGACATTGAGGAGATTGGAGCAACAGCTGAGAATGCAGCCATTTACAATAGGAATTTGTTGAGAAGACAATTGGCTAATGAAGGAACTCCTCTAAGGCCAAATATGAGACTTGAAGTTTATCTTTTTGCTCTCTTTAATGAAGACATGAAGCCTGGACCAACCTCTGAAAGAAACTATGGTCTCTATCAACCTGATGGAACCATGGCTTACAATGTTGGCCTTTCCGCCTTGTTGACTTCTTCTTCAACTTCTTCTGCATCCATCTCCCTATCTTCTTCGGCCTCAAAGACAAGAACAATGGAATATAAAAGCTTAGTGTATTGGATGTTTGTGTATTTGCTGCTGAGCTTCCAGATTATTCCGACGAGAAGACCATTTTAG

Protein sequence

MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTFANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLMANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFLSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFAMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTRTMEYKSLVYWMFVYLLLSFQIIPTRRPF
Homology
BLAST of Lcy04g022950 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.4e-95
Identity = 170/340 (50.00%), Postives = 235/340 (69.12%), Query Frame = 0

Query: 4   LFLLFSLTF----SGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTT 63
           L  L SLT     +   S+G+NYGQ+GDNLP P  V+ L+ S+  +KV++YD NPQIL  
Sbjct: 11  LLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKA 70

Query: 64  FANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTL 123
           F+N+  E I+ + NE LS++ DP +A+ W+  ++ P+ PA  IT I +GNEI   ND++L
Sbjct: 71  FSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSL 130

Query: 124 MANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQF 183
             NL+PAM  +H AL   GL   I V+T  SL++L+ SFPPSAG F+  +   ++  L+F
Sbjct: 131 TTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEF 190

Query: 184 LSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 243
              T +PF INAYP+FAYK NP  + LD+ L  P   +VDP+T  +YDNML+AQ+DAV  
Sbjct: 191 HRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYS 250

Query: 244 AMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANE--GTPLRPNM 303
           A+A  GF  + V +SETGWPSKGD +E+GAT ENA  YN NL++  ++ +   TPL+PN 
Sbjct: 251 ALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNN 310

Query: 304 RLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLS 338
            L +Y+FALFNE++KPGPTSERNYGL++PDGT AY++G +
Sbjct: 311 DLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFA 350

BLAST of Lcy04g022950 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 6.1e-91
Identity = 173/358 (48.32%), Postives = 242/358 (67.60%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           ++ LFL  S    G + +GINYGQ+ +NLP P +V  LL SL I++V++YD +P +L +F
Sbjct: 13  LLLLFLTLSERIKG-QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 72

Query: 61  ANSKTELIVTIENEMLSQL-MDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTL 120
           +NS+ + ++ + NE L  +  DP +A  W+   ++P+    RIT I VGNEIF  ND  L
Sbjct: 73  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 132

Query: 121 MANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQF 180
           + +L+PAM S++ AL  LGL   + V++  SL +L  S+PPS+GSFK +   Y+   L F
Sbjct: 133 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 192

Query: 181 LSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 240
            S  ++PF INAYP+FAYKD+P  + L+Y L  P   MVDP+T L+YDNML+AQVDA+  
Sbjct: 193 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 252

Query: 241 AMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRL 300
           A+  LG   IEVR+SETGWPSKGD  EIGA+ ENAA+YN NLL+     +GTP + ++ +
Sbjct: 253 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 312

Query: 301 EVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL-----TSSSTSSASISL 353
           +VY+FALFNE++KPGP SERNYGL+ PDG   YNVG+   L     TS +T+   ++L
Sbjct: 313 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKILNL 369

BLAST of Lcy04g022950 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.3e-88
Identity = 174/359 (48.47%), Postives = 235/359 (65.46%), Query Frame = 0

Query: 2   IYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTFA 61
           ++   LFSL    V S+GINYGQV +NLPPP  V+ LL S+  +KV++YD +PQ L  FA
Sbjct: 12  LFCLALFSLPLI-VSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRAFA 71

Query: 62  NSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLMA 121
            S  EL V + NE L+Q+ DP +A  WV  +++ Y P  +I  I VGNE+ T N + L A
Sbjct: 72  GSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSALTA 131

Query: 122 NLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFLS 181
            L PAM SIHGAL   GL   I V+T  SLA+L+ S+PPSA SF+  +   ++  L F  
Sbjct: 132 ALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDFHV 191

Query: 182 STKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFAM 241
            T +P  INAYP+FAY++NP  +SLD+ L  P     DP +  +YDNML+AQVDAV  A+
Sbjct: 192 KTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYHAL 251

Query: 242 ARLG--FGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANE-GTPLRPNMR 301
             +G  +  + + VSETGWPS GD +E+GAT +NA  YN NL++  ++ +  TP+RP   
Sbjct: 252 DAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPECD 311

Query: 302 LEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSAS 358
           L +++FALFNE+MKPGPTSERNYGL+ PDGT  Y++G+    T SS S +S S   S+S
Sbjct: 312 LTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGSSS 369

BLAST of Lcy04g022950 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-76
Identity = 153/356 (42.98%), Postives = 222/356 (62.36%), Query Frame = 0

Query: 2   IYLFLLFSLTFSGVES---LGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILT 61
           IY  L+F   F    +   +G+NYGQV DNLPPP + ++LL S  I KVR+Y  +P I+ 
Sbjct: 7   IYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIK 66

Query: 62  TFANSKTELIVTIEN-EMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDT 121
             A +   +++   N ++ S   DP  A QW+ +++ P++PA++I  I VGNEI   ND 
Sbjct: 67  ALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDP 126

Query: 122 TLMANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFL 181
            L+  L+PAM ++  AL  + LG  I VST  S+ VL  S PPS+GSF +   T +   L
Sbjct: 127 NLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGIL 186

Query: 182 QFLSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAV 241
           QFLS T +PF IN YP+FAY+ +P   +L + L  P +  VD  T + Y NM  AQVDAV
Sbjct: 187 QFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAV 246

Query: 242 IFAMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNM 301
             A+  +GF  +E+ V+ETGW S+GD  E+GA+ +NA  YN NL+    +  GTPL P  
Sbjct: 247 HSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGK 306

Query: 302 RLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLS 354
            ++ Y+FAL++E++KPGP+SER +GL++ D +M Y+VGL+   +SS T S  ++ S
Sbjct: 307 PVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGKVTSS 362

BLAST of Lcy04g022950 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.3e-72
Identity = 152/360 (42.22%), Postives = 221/360 (61.39%), Query Frame = 0

Query: 2   IYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTFA 61
           I++ +L  L F     +GI YG+  DNLP P++V EL+  L I  VRIYD N  +L  FA
Sbjct: 11  IFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFA 70

Query: 62  NSKTELIVTIENEMLSQLMDPQQAVQ-WVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 121
           N+  EL++ + N  L      Q  V  W++ +I PY+P+ +IT I+VG E+    D    
Sbjct: 71  NTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA-T 130

Query: 122 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 181
             ++PAM +IH AL + GL   I +S+  SLA+L  SFPPS+ SF  + S ++   L+FL
Sbjct: 131 GLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFL 190

Query: 182 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 241
              ++PF I+ YPY+AY+D+   + L+YAL    S +VDP+T L Y NM  AQ+DA+ FA
Sbjct: 191 VENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFA 250

Query: 242 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 301
           +  + F  ++V V+E+GWPSKG  +E  AT ENA  YN NL+R  + + GTP +P   ++
Sbjct: 251 LTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEID 310

Query: 302 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLT-----SSSTSSASISLSSS 356
           VYLF+LFNE+ KPG  SERN+G++  +GT  Y +  +   T     ++ST+  S S SSS
Sbjct: 311 VYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSS 369

BLAST of Lcy04g022950 vs. ExPASy TrEMBL
Match: A0A6J1CZ37 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111015800 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.1e-202
Identity = 368/388 (94.85%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI++FLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MIHIFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM
Sbjct: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIH AL QLGLGSYIHVSTPTSLAVLEESFPPSAGSFK+QVS YISQFLQFL
Sbjct: 121 ANLVPAMISIHSALAQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKTQVSPYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNPN ISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPNKISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFG 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD +EIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDDDEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSSTSSASISLSSSASKT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSTSSASISLSSSASKTA 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
            MEY+SLVYWMFVYLL+  QII TRRPF
Sbjct: 361 AMEYQSLVYWMFVYLLVGIQIILTRRPF 388

BLAST of Lcy04g022950 vs. ExPASy TrEMBL
Match: A0A6J1EDF3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111433179 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.4e-199
Identity = 361/388 (93.04%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI LFLLFSLT SGVE LGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MILLFLLFSLTASGVECLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEML+QLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLTQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTALM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIHGAL QLGLGSY+HVSTPTSLAVLEESFPPSAGSFKSQV+TYISQFLQFL
Sbjct: 121 ANLVPAMISIHGALAQLGLGSYVHVSTPTSLAVLEESFPPSAGSFKSQVATYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNP+ ISLDYALLNPMSYMVDPST+LNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPDKISLDYALLNPMSYMVDPSTRLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD++EIGATA+NAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDLDEIGATAQNAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSSTSSASISLSSSA+KT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSTSSASISLSSSATKTT 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
           T E+KSLVYWMFVYLL+SF+ I TR+ F
Sbjct: 361 TTEFKSLVYWMFVYLLISFKTILTRQLF 388

BLAST of Lcy04g022950 vs. ExPASy TrEMBL
Match: A0A6J1IS87 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111478836 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 4.6e-198
Identity = 358/388 (92.27%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI LFLLFSLT SGVE LGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MILLFLLFSLTVSGVECLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEML+QLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM
Sbjct: 61  ANSKTELIVTIENEMLTQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIHGAL QLGLGSY+HVSTPTSLAVLEESFPPSAGSFKSQV++YISQFLQFL
Sbjct: 121 ANLVPAMISIHGALAQLGLGSYVHVSTPTSLAVLEESFPPSAGSFKSQVASYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFA+KDNP+ ISLDYALLNPMSYMVDPST+LNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAFKDNPDKISLDYALLNPMSYMVDPSTRLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD++EIGATA+NAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDVDEIGATAQNAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+S+STSSASISLSSSA+KT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSTSTSSASISLSSSATKTT 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
             E+KSLVYWMFVYLL+SF+ I TR+ F
Sbjct: 361 RTEFKSLVYWMFVYLLISFKTIFTRQLF 388

BLAST of Lcy04g022950 vs. ExPASy TrEMBL
Match: A0A0A0KI46 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G523410 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 4.6e-198
Identity = 360/388 (92.78%), Postives = 373/388 (96.13%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI+ FLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MIHFFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEMLSQLMDPQQA+QWVATHIKPYFPA+RITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLSQLMDPQQALQWVATHIKPYFPASRITGIAVGNEIFTGNDTVLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           +NLVPAMI+IH AL +LGLGSYIHVSTPTSLAVLE SFPPSAGSFK+Q STYISQFLQFL
Sbjct: 121 SNLVPAMINIHNALARLGLGSYIHVSTPTSLAVLENSFPPSAGSFKTQFSTYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNPN ISLDYALLNPMSYMVDP TKLNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPNKISLDYALLNPMSYMVDPYTKLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MA LGFGGIEVRVSETGWPSKGD +EIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MANLGFGGIEVRVSETGWPSKGDYDEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL-TSSSTSSASISLSSSASKT 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL +SSSTSSASISLSSSA+KT
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSSTSSASISLSSSATKT 360

Query: 361 RTMEYKSLVYWMFVYLLLSFQIIPTRRP 388
            +MEYKSLVYWMFVYLL+S +II TRRP
Sbjct: 361 ISMEYKSLVYWMFVYLLISLEIILTRRP 388

BLAST of Lcy04g022950 vs. ExPASy TrEMBL
Match: A0A1S3AYS7 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484364 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.6e-197
Identity = 357/389 (91.77%), Postives = 372/389 (95.63%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI+ FLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MIHFFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEMLSQLMDPQQA++WVATHIKPYFPA+RITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLSQLMDPQQALEWVATHIKPYFPASRITGIAVGNEIFTGNDTVLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           +NLVPAMI+IH AL QLGLGSYIHVSTPTSLAVLE SFPPSAGSFK+Q STYISQFLQFL
Sbjct: 121 SNLVPAMINIHSALAQLGLGSYIHVSTPTSLAVLENSFPPSAGSFKTQFSTYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNPN ISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPNKISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD +EIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDYDEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSS--TSSASISLSSSASK 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSS  +SSASISLSSSA+K
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSSTSSSASISLSSSATK 360

Query: 361 TRTMEYKSLVYWMFVYLLLSFQIIPTRRP 388
           T +MEYKSL YWMF YL++S +II TRRP
Sbjct: 361 TISMEYKSLAYWMFAYLVISLEIILTRRP 389

BLAST of Lcy04g022950 vs. NCBI nr
Match: XP_022146648.1 (glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Momordica charantia])

HSP 1 Score: 715.7 bits (1846), Expect = 2.2e-202
Identity = 368/388 (94.85%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI++FLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MIHIFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM
Sbjct: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIH AL QLGLGSYIHVSTPTSLAVLEESFPPSAGSFK+QVS YISQFLQFL
Sbjct: 121 ANLVPAMISIHSALAQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKTQVSPYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNPN ISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPNKISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFG 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD +EIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDDDEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSSTSSASISLSSSASKT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSTSSASISLSSSASKTA 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
            MEY+SLVYWMFVYLL+  QII TRRPF
Sbjct: 361 AMEYQSLVYWMFVYLLVGIQIILTRRPF 388

BLAST of Lcy04g022950 vs. NCBI nr
Match: XP_022925901.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata])

HSP 1 Score: 704.5 bits (1817), Expect = 5.0e-199
Identity = 361/388 (93.04%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI LFLLFSLT SGVE LGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MILLFLLFSLTASGVECLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEML+QLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLTQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTALM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIHGAL QLGLGSY+HVSTPTSLAVLEESFPPSAGSFKSQV+TYISQFLQFL
Sbjct: 121 ANLVPAMISIHGALAQLGLGSYVHVSTPTSLAVLEESFPPSAGSFKSQVATYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNP+ ISLDYALLNPMSYMVDPST+LNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPDKISLDYALLNPMSYMVDPSTRLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD++EIGATA+NAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDLDEIGATAQNAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSSTSSASISLSSSA+KT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSTSSASISLSSSATKTT 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
           T E+KSLVYWMFVYLL+SF+ I TR+ F
Sbjct: 361 TTEFKSLVYWMFVYLLISFKTILTRQLF 388

BLAST of Lcy04g022950 vs. NCBI nr
Match: XP_023544619.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.6 bits (1812), Expect = 1.9e-198
Identity = 359/388 (92.53%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI +FLLFSLT SGVE LGINYGQVGDNLPPPDKVL+LLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MILIFLLFSLTASGVECLGINYGQVGDNLPPPDKVLQLLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEML+QLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLTQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTALM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIHGAL QLGLGSY+HVSTPTSLAVLEESFPPSAGSFKSQV+TYISQFLQFL
Sbjct: 121 ANLVPAMISIHGALAQLGLGSYVHVSTPTSLAVLEESFPPSAGSFKSQVATYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNP+ ISLDYALLNPMSYMVDPST+LNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPDKISLDYALLNPMSYMVDPSTRLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD++EIGATA+NAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDLDEIGATAQNAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+SSSTSSASISLSSSA+KT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSTSSASISLSSSATKTT 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
           T E+KSLVYWMFVYLL+SF+ I TR+ F
Sbjct: 361 TTEFKSLVYWMFVYLLISFKTILTRQLF 388

BLAST of Lcy04g022950 vs. NCBI nr
Match: XP_022979095.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima])

HSP 1 Score: 700.3 bits (1806), Expect = 9.5e-198
Identity = 358/388 (92.27%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI LFLLFSLT SGVE LGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MILLFLLFSLTVSGVECLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEML+QLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM
Sbjct: 61  ANSKTELIVTIENEMLTQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           ANLVPAMISIHGAL QLGLGSY+HVSTPTSLAVLEESFPPSAGSFKSQV++YISQFLQFL
Sbjct: 121 ANLVPAMISIHGALAQLGLGSYVHVSTPTSLAVLEESFPPSAGSFKSQVASYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFA+KDNP+ ISLDYALLNPMSYMVDPST+LNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAFKDNPDKISLDYALLNPMSYMVDPSTRLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MARLGF GIEVRVSETGWPSKGD++EIGATA+NAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MARLGFDGIEVRVSETGWPSKGDVDEIGATAQNAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSASKTR 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL+S+STSSASISLSSSA+KT 
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSTSTSSASISLSSSATKTT 360

Query: 361 TMEYKSLVYWMFVYLLLSFQIIPTRRPF 389
             E+KSLVYWMFVYLL+SF+ I TR+ F
Sbjct: 361 RTEFKSLVYWMFVYLLISFKTIFTRQLF 388

BLAST of Lcy04g022950 vs. NCBI nr
Match: XP_011658291.1 (glucan endo-1,3-beta-glucosidase 11 isoform X2 [Cucumis sativus] >KGN49400.1 hypothetical protein Csa_002830 [Cucumis sativus])

HSP 1 Score: 700.3 bits (1806), Expect = 9.5e-198
Identity = 360/388 (92.78%), Postives = 373/388 (96.13%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           MI+ FLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQIL TF
Sbjct: 1   MIHFFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILATF 60

Query: 61  ANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTLM 120
           ANSKTELIVTIENEMLSQLMDPQQA+QWVATHIKPYFPA+RITGIAVGNEIFTGNDT LM
Sbjct: 61  ANSKTELIVTIENEMLSQLMDPQQALQWVATHIKPYFPASRITGIAVGNEIFTGNDTVLM 120

Query: 121 ANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQFL 180
           +NLVPAMI+IH AL +LGLGSYIHVSTPTSLAVLE SFPPSAGSFK+Q STYISQFLQFL
Sbjct: 121 SNLVPAMINIHNALARLGLGSYIHVSTPTSLAVLENSFPPSAGSFKTQFSTYISQFLQFL 180

Query: 181 SSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIFA 240
           SSTKAPFWINAYPYFAYKDNPN ISLDYALLNPMSYMVDP TKLNYDNMLYAQVDAVIFA
Sbjct: 181 SSTKAPFWINAYPYFAYKDNPNKISLDYALLNPMSYMVDPYTKLNYDNMLYAQVDAVIFA 240

Query: 241 MARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300
           MA LGFGGIEVRVSETGWPSKGD +EIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE
Sbjct: 241 MANLGFGGIEVRVSETGWPSKGDYDEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRLE 300

Query: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL-TSSSTSSASISLSSSASKT 360
           VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL +SSSTSSASISLSSSA+KT
Sbjct: 301 VYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLSSSSSTSSASISLSSSATKT 360

Query: 361 RTMEYKSLVYWMFVYLLLSFQIIPTRRP 388
            +MEYKSLVYWMFVYLL+S +II TRRP
Sbjct: 361 ISMEYKSLVYWMFVYLLISLEIILTRRP 388

BLAST of Lcy04g022950 vs. TAIR 10
Match: AT1G30080.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 505.4 bits (1300), Expect = 4.2e-143
Identity = 263/390 (67.44%), Postives = 310/390 (79.49%), Query Frame = 0

Query: 4   LFLLFSLT-------FSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQI 63
           + LLFSLT       F  V SLGINYGQVGDNLPPPDKVL+LL+SL I+K RIYDTNP++
Sbjct: 15  VLLLFSLTFLEHGLLFQRVSSLGINYGQVGDNLPPPDKVLQLLSSLHINKTRIYDTNPRV 74

Query: 64  LTTFANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGND 123
           LT+FANS  EL VT+ENEML  L+DPQQA+QWV T IKPYFPA +I GIAVGNE++T +D
Sbjct: 75  LTSFANSNIELFVTVENEMLPSLVDPQQALQWVTTRIKPYFPATKIGGIAVGNELYTDDD 134

Query: 124 TTLMANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQF 183
           ++L+  L+PAM+SIHGAL Q GL  YI VSTP SL+VL+ES+PPSAG F+ +V+  ++Q 
Sbjct: 135 SSLIGYLMPAMMSIHGALVQTGLDKYIQVSTPNSLSVLQESYPPSAGCFRPEVAGVMTQL 194

Query: 184 LQFLSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDA 243
           L FL +T +PFWINAYPYFAYKD+P  I LDY L NP   MVDP TK  YDNMLYAQVDA
Sbjct: 195 LGFLRNTNSPFWINAYPYFAYKDSPTKIPLDYVLFNPNPGMVDPYTKYRYDNMLYAQVDA 254

Query: 244 VIFAMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPN 303
           VIFAMARLGF  IEV VSETGWPSKGD +E+GAT  NAA+YN+N+LRRQL NEGTPLRPN
Sbjct: 255 VIFAMARLGFKDIEVGVSETGWPSKGDGDEVGATVANAAVYNKNILRRQLQNEGTPLRPN 314

Query: 304 MRLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGL--SALLTSSSTSSAS--ISL 363
           +  +VYLFALFNED+KPGPTSERNYGLYQPD TM YNVGL  S+ LTS+ST+S++  ISL
Sbjct: 315 LSFDVYLFALFNEDLKPGPTSERNYGLYQPDETMTYNVGLLSSSSLTSTSTTSSTSIISL 374

Query: 364 SSSASKTRTMEYKSLVYWMFVYLLLSFQII 383
           +SSAS       + L+YW  VYLL    +I
Sbjct: 375 TSSASTALKKGKQRLMYWTCVYLLAIHMLI 404

BLAST of Lcy04g022950 vs. TAIR 10
Match: AT4G18340.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.1e-135
Identity = 246/390 (63.08%), Postives = 308/390 (78.97%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSG-----VESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQ 60
           +I++FLL SL FSG     V SLGINYGQVG+NLP PDKV+ LL SL+I+K RIYDTNPQ
Sbjct: 12  LIHVFLLLSLVFSGNILQTVTSLGINYGQVGNNLPSPDKVINLLRSLRITKTRIYDTNPQ 71

Query: 61  ILTTFANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGN 120
           IL+ FANS  E+IVTIEN++L  L DPQQA QWV +HIKPY PA RITGI VGNE+FT +
Sbjct: 72  ILSAFANSNIEIIVTIENQVLPLLQDPQQATQWVDSHIKPYVPATRITGIMVGNELFTDD 131

Query: 121 DTTLMANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQ 180
           D++L+  ++PA+I+IH AL QLGL  YI VS+P+SLAVL ES+PPSAGSFK +VS+ + Q
Sbjct: 132 DSSLIGYMMPAIINIHKALVQLGLDRYIQVSSPSSLAVLGESYPPSAGSFKPEVSSVMQQ 191

Query: 181 FLQFLSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVD 240
            L FL +TK+PFWINAYPYFAYKDNP  I +DY L N    M DP+T+L+YDNM+YAQVD
Sbjct: 192 LLDFLEATKSPFWINAYPYFAYKDNPQEIPVDYVLFNRNIGMTDPNTRLHYDNMMYAQVD 251

Query: 241 AVIFAMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRP 300
           AV FA A+LG+  IEVRV+ETGWPSKGD+ EIGA+  NAA YNRNL+ RQ A EGTP R 
Sbjct: 252 AVAFAAAKLGYRNIEVRVAETGWPSKGDVGEIGASPVNAATYNRNLMMRQFAGEGTPARR 311

Query: 301 NMRLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSS 360
           N RL+VY+FALFNEDMKPGPTSE+NYG++QPDG++AYN+G S + T+++ +S S++ SSS
Sbjct: 312 NARLDVYIFALFNEDMKPGPTSEKNYGIFQPDGSLAYNLGFSTMSTTTA-NSESVTYSSS 371

Query: 361 ASKTRTMEYKSLVYWMFVYLLLSFQIIPTR 386
           A+K +    +SL YW  + +L   Q++  R
Sbjct: 372 ATKAK----RSLEYWT-ILILAMVQVVMLR 395

BLAST of Lcy04g022950 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.7e-96
Identity = 170/340 (50.00%), Postives = 235/340 (69.12%), Query Frame = 0

Query: 4   LFLLFSLTF----SGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTT 63
           L  L SLT     +   S+G+NYGQ+GDNLP P  V+ L+ S+  +KV++YD NPQIL  
Sbjct: 11  LLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKA 70

Query: 64  FANSKTELIVTIENEMLSQLMDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTL 123
           F+N+  E I+ + NE LS++ DP +A+ W+  ++ P+ PA  IT I +GNEI   ND++L
Sbjct: 71  FSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSL 130

Query: 124 MANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQF 183
             NL+PAM  +H AL   GL   I V+T  SL++L+ SFPPSAG F+  +   ++  L+F
Sbjct: 131 TTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEF 190

Query: 184 LSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 243
              T +PF INAYP+FAYK NP  + LD+ L  P   +VDP+T  +YDNML+AQ+DAV  
Sbjct: 191 HRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYS 250

Query: 244 AMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANE--GTPLRPNM 303
           A+A  GF  + V +SETGWPSKGD +E+GAT ENA  YN NL++  ++ +   TPL+PN 
Sbjct: 251 ALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNN 310

Query: 304 RLEVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLS 338
            L +Y+FALFNE++KPGPTSERNYGL++PDGT AY++G +
Sbjct: 311 DLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFA 350

BLAST of Lcy04g022950 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-92
Identity = 173/358 (48.32%), Postives = 242/358 (67.60%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           ++ LFL  S    G + +GINYGQ+ +NLP P +V  LL SL I++V++YD +P +L +F
Sbjct: 13  LLLLFLTLSERIKG-QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 72

Query: 61  ANSKTELIVTIENEMLSQL-MDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTL 120
           +NS+ + ++ + NE L  +  DP +A  W+   ++P+    RIT I VGNEIF  ND  L
Sbjct: 73  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 132

Query: 121 MANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQF 180
           + +L+PAM S++ AL  LGL   + V++  SL +L  S+PPS+GSFK +   Y+   L F
Sbjct: 133 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 192

Query: 181 LSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 240
            S  ++PF INAYP+FAYKD+P  + L+Y L  P   MVDP+T L+YDNML+AQVDA+  
Sbjct: 193 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 252

Query: 241 AMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRL 300
           A+  LG   IEVR+SETGWPSKGD  EIGA+ ENAA+YN NLL+     +GTP + ++ +
Sbjct: 253 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 312

Query: 301 EVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALL-----TSSSTSSASISL 353
           +VY+FALFNE++KPGP SERNYGL+ PDG   YNVG+   L     TS +T+   ++L
Sbjct: 313 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKILNL 369

BLAST of Lcy04g022950 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.7e-92
Identity = 171/358 (47.77%), Postives = 242/358 (67.60%), Query Frame = 0

Query: 1   MIYLFLLFSLTFSGVESLGINYGQVGDNLPPPDKVLELLTSLKISKVRIYDTNPQILTTF 60
           ++ LFL  S    G + +GINYGQ+ +NLP P +V  LL SL I++V++YD +P +L +F
Sbjct: 13  LLLLFLTLSERIKG-QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 72

Query: 61  ANSKTELIVTIENEMLSQL-MDPQQAVQWVATHIKPYFPAARITGIAVGNEIFTGNDTTL 120
           +NS+ + ++ + NE L  +  DP +A  W+   ++P+    RIT I VGNEIF  ND  L
Sbjct: 73  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 132

Query: 121 MANLVPAMISIHGALGQLGLGSYIHVSTPTSLAVLEESFPPSAGSFKSQVSTYISQFLQF 180
           + +L+PAM S++ AL  LGL   + V++  SL +L  S+PPS+GSFK +   Y+   L F
Sbjct: 133 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 192

Query: 181 LSSTKAPFWINAYPYFAYKDNPNIISLDYALLNPMSYMVDPSTKLNYDNMLYAQVDAVIF 240
            S  ++PF INAYP+FAYKD+P  + L+Y L  P   MVDP+T L+YDNML+AQVDA+  
Sbjct: 193 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 252

Query: 241 AMARLGFGGIEVRVSETGWPSKGDIEEIGATAENAAIYNRNLLRRQLANEGTPLRPNMRL 300
           A+  LG   IEVR+SETGWPSKGD  EIGA+ ENAA+YN NLL+     +GTP + ++ +
Sbjct: 253 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 312

Query: 301 EVYLFALFNEDMKPGPTSERNYGLYQPDGTMAYNVGLSALLTSSSTSSASISLSSSAS 358
           +VY+FALFNE++KPGP SERNYGL+ PDG   YNVG+   L     +S + ++  ++S
Sbjct: 313 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKVNSS 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8682.4e-9550.00Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Q9ZQG96.1e-9148.32Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500... [more]
Q9FHX51.3e-8848.47Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100... [more]
Q9M0691.9e-7642.98Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE51.3e-7242.22Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
A0A6J1CZ371.1e-20294.85(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111015800 ... [more]
A0A6J1EDF32.4e-19993.04(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111433179 P... [more]
A0A6J1IS874.6e-19892.27(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111478836 PE=... [more]
A0A0A0KI464.6e-19892.78(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G523410 PE=3... [more]
A0A1S3AYS78.6e-19791.77(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484364 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022146648.12.2e-20294.85glucan endo-1,3-beta-glucosidase 11-like isoform X1 [Momordica charantia][more]
XP_022925901.15.0e-19993.04glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata][more]
XP_023544619.11.9e-19892.53glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo][more]
XP_022979095.19.5e-19892.27glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima][more]
XP_011658291.19.5e-19892.78glucan endo-1,3-beta-glucosidase 11 isoform X2 [Cucumis sativus] >KGN49400.1 hyp... [more]
Match NameE-valueIdentityDescription
AT1G30080.14.2e-14367.44Glycosyl hydrolase superfamily protein [more]
AT4G18340.11.1e-13563.08Glycosyl hydrolase superfamily protein [more]
AT1G32860.11.7e-9650.00Glycosyl hydrolase superfamily protein [more]
AT2G27500.14.4e-9248.32Glycosyl hydrolase superfamily protein [more]
AT2G27500.25.7e-9247.77Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 18..334
e-value: 2.0E-85
score: 287.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 16..341
e-value: 8.1E-119
score: 398.3
NoneNo IPR availablePANTHERPTHR32227:SF349GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 11..367
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 11..367
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 18..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g022950.1Lcy04g022950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds