Lcy04g021380 (gene) Sponge gourd (P93075) v1

BLAST of Lcy04g021380 vs. ExPASy Swiss-Prot
Match: Q9FZA2 (Non-classical arabinogalactan protein 31 OS=Arabidopsis thaliana OX=3702 GN=AGP31 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.6e-40
Identity = 114/247 (46.15%), Postives = 145/247 (58.70%), Query Frame = 0

Query: 31  PPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHG 90
           PP      PP KPP       + PT APV PPT  P+ PPV+PP + P            
Sbjct: 126 PPTKAPVKPPTKPPV--KPPVYPPTKAPVKPPTKPPVKPPVYPPTKAP-------VKPPT 185

Query: 91  QPPVHPPANAPSHHLPPTHPPAHSPAPAHHHRDVLPLPPPAHSPAPIYPPKSRLPRSFIS 150
           +PPV PP + P+   PP  PP + P  A     V P   P  +P P+YPPK    RS ++
Sbjct: 186 KPPVKPPVSPPAK--PPVKPPVYPPTKAPVKPPVSPPTKPPVTP-PVYPPK--FNRSLVA 245

Query: 151 VQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAI 210
           V+G VYCKSCKYA  +TL+GA P+ GATVKLVC++ K    +T TTDKNGYF + APK +
Sbjct: 246 VRGTVYCKSCKYAAFNTLLGAKPIEGATVKLVCKSKKNITAET-TTDKNGYFLLLAPKTV 305

Query: 211 TSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQ----KSYIDANKLPFVLYSVGP 270
           T++ F  C+V LV S    CSK S L GG  GA L+P+    KS +  NKL + L++VGP
Sbjct: 306 TNFGFRGCRVYLVKSKDYKCSKVSKLFGGDVGAELKPEKKLGKSTVVVNKLVYGLFNVGP 357

Query: 271 FAFEPTC 274
           FAF P+C
Sbjct: 366 FAFNPSC 357

BLAST of Lcy04g021380 vs. ExPASy Swiss-Prot
Match: P93013 (Non-classical arabinogalactan protein 30 OS=Arabidopsis thaliana OX=3702 GN=AGP30 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-31
Identity = 96/218 (44.04%), Postives = 124/218 (56.88%), Query Frame = 0

Query: 62  PTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPANAPSHHLPPTHPPAHSPAPAHHH 121
           P HLP+ PP+  P  PP       A    + P +PPA AP     PT PPA +P      
Sbjct: 40  PQHLPL-PPIKLPTLPP-------AKAPIKLPAYPPAKAPIK--LPTLPPAKAPIK---- 99

Query: 122 RDVLPLPPPAHSPA--PIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATV 181
              LP  PP   P   P+YPPK    ++ ++V+GVVYCK+CKYAGV+ + GA PV  A V
Sbjct: 100 ---LPTLPPIKPPVLPPVYPPKYN--KTLVAVRGVVYCKACKYAGVNNVQGAKPVKDAVV 159

Query: 182 KLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGG 241
           +LVC+N K  + +T  TDKNGYF + APK +T+Y    C+  LV SP   CSK S+LH G
Sbjct: 160 RLVCKNKKNSISET-KTDKNGYFMLLAPKTVTNYDIKGCRAFLVKSPDTKCSKVSSLHDG 219

Query: 242 AAGAALRPQK----SYIDANKLPFVLYSVGPFAFEPTC 274
             G+ L+P      S        + +Y+VGPFAFEPTC
Sbjct: 220 GKGSVLKPVLKPGFSSTIMRWFKYSVYNVGPFAFEPTC 237

BLAST of Lcy04g021380 vs. ExPASy Swiss-Prot
Match: Q03211 (Pistil-specific extensin-like protein OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-18
Identity = 101/266 (37.97%), Postives = 125/266 (46.99%), Query Frame = 0

Query: 31  PPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHG 90
           PP   A  PP  PPA        P+P P  PP   P   P   P  PP            
Sbjct: 160 PPDQSAKQPPQPPPAKQ------PSPPPPPPPVKAPSPSPAKQPPPPPPPVKAPSPSPAT 219

Query: 91  QPPVH----PPANAPSHHLPPTHPPAH------SPAPAHHHRDVLPLP-PPAHSP----- 150
           QPP      PP    S  LPP  P A+      SP+PA     + P P PPA+ P     
Sbjct: 220 QPPTKQPPPPPRAKKSPLLPPPPPVAYPPVMTPSPSPAAEPPIIAPFPSPPANPPLIPRR 279

Query: 151 --APIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQ 210
              P+  P   L +  I V G+VYCKSC   GV TL+ A+ + GA VKL+C   K  +VQ
Sbjct: 280 PAPPVVKPLPPLGKPPI-VSGLVYCKSCNSYGVPTLLNASLLQGAVVKLICYGKK-TMVQ 339

Query: 211 TATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALR---PQK 270
            ATTD  G F I  PK++T+    KCKV LV SP+ +C+ P+  +GG +G  L+   P K
Sbjct: 340 WATTDNKGEFRI-MPKSLTTADVGKCKVYLVKSPNPNCNVPTNFNGGKSGGLLKPLLPPK 399

BLAST of Lcy04g021380 vs. ExPASy TrEMBL
Match: A0A6J1EEX2 (non-classical arabinogalactan protein 30-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433591 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 8.5e-114
Identity = 231/302 (76.49%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHH-HHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPAHHH HHHHAP 
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLATSPHHYAAVPPAQPPAHHHYHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTHPPAHS 120
           PAPV+PPTHL    PVHPPAQPPSH HHHH HVH QPPVHPP  NAPSHHLPPTH P H 
Sbjct: 61  PAPVSPPTHL----PVHPPAQPPSHNHHHH-HVHSQPPVHPPVHNAPSHHLPPTHLPVHP 120

Query: 121 P-------APAH-------------HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGV 180
           P       AP+H             HHRDV PL PPAHSP+P+YPPK RL RSFISVQGV
Sbjct: 121 PVHPPVNNAPSHHLPPTKALAPGHEHHRDVRPLLPPAHSPSPMYPPKPRLLRSFISVQGV 180

Query: 181 VYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYA 240
           VYCKSCKYAGVDTL GATPVAGA+VKL+CQNTK+PLVQTATTD NGYFFI APKAITSYA
Sbjct: 181 VYCKSCKYAGVDTLNGATPVAGASVKLICQNTKHPLVQTATTDNNGYFFINAPKAITSYA 240

Query: 241 FHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCT 277
           FHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPFVLYSVGPFAFEPTC 
Sbjct: 241 FHKCKVLLGSSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPFVLYSVGPFAFEPTCP 297

BLAST of Lcy04g021380 vs. ExPASy TrEMBL
Match: A0A6J1IR37 (non-classical arabinogalactan protein 31-like OS=Cucurbita maxima OX=3661 GN=LOC111478609 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 6.1e-112
Identity = 215/269 (79.93%), Postives = 229/269 (85.13%), Query Frame = 0

Query: 9   FFFAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPTHLPIH 68
           FFF +F    ADD  A ETLA PPHHYAA+PP      HHHHHH+PTPAPVAPPTHLP+ 
Sbjct: 9   FFFLLFFAIFADDTTAAETLAAPPHHYAAVPPT-----HHHHHHSPTPAPVAPPTHLPL- 68

Query: 69  PPVHPPAQPPSHRHHHHAHVHGQPPVHPPANAPSHHLPPTHPPAHSPAPA-HHHRDVLPL 128
              HPPAQPPSH HHHH H+H QPPVHP    P HHLPPTHPP H+PAP  HHH DV PL
Sbjct: 69  ---HPPAQPPSHHHHHH-HIHTQPPVHP----PHHHLPPTHPPVHAPAPGHHHHHDVHPL 128

Query: 129 PPPAHSPAPIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTK 188
           PPP HSPAP++PPK RL RSFISVQGVVYCKSCKYAGVDTL+GAT VAGATVKL+CQNTK
Sbjct: 129 PPPTHSPAPLHPPKPRLLRSFISVQGVVYCKSCKYAGVDTLLGATTVAGATVKLICQNTK 188

Query: 189 YPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRP 248
           YPLVQTATTDKNGYFFITAPKA+TSYAFHKCKV+L SSP+ SCSKPSALHGGAAGA LRP
Sbjct: 189 YPLVQTATTDKNGYFFITAPKAVTSYAFHKCKVVLGSSPTPSCSKPSALHGGAAGAPLRP 248

Query: 249 QKSYIDANKLPFVLYSVGPFAFEPTCTHH 277
           QKSYIDANKLP+VLYSVGPFAFEPTC HH
Sbjct: 249 QKSYIDANKLPYVLYSVGPFAFEPTCAHH 263

BLAST of Lcy04g021380 vs. ExPASy TrEMBL
Match: A0A6J1EEG4 (non-classical arabinogalactan protein 30-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433591 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 6.1e-112
Identity = 226/283 (79.86%), Postives = 241/283 (85.16%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHH-HHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPAHHH HHHHAP 
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLATSPHHYAAVPPAQPPAHHHYHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTHPPAHS 120
           PAPV+PPTHL    PVHPPAQPPSH HHHH HVH  PPVHPP  NAPSHHLPPT     +
Sbjct: 61  PAPVSPPTHL----PVHPPAQPPSHNHHHH-HVHVHPPVHPPVNNAPSHHLPPT----KA 120

Query: 121 PAPAH-HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATP 180
            AP H HHRDV PL PPAHSP+P+YPPK RL RSFISVQGVVYCKSCKYAGVDTL GATP
Sbjct: 121 LAPGHEHHRDVRPLLPPAHSPSPMYPPKPRLLRSFISVQGVVYCKSCKYAGVDTLNGATP 180

Query: 181 VAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKP 240
           VAGA+VKL+CQNTK+PLVQTATTD NGYFFI APKAITSYAFHKCKVLL SSP A+CSKP
Sbjct: 181 VAGASVKLICQNTKHPLVQTATTDNNGYFFINAPKAITSYAFHKCKVLLGSSPVAACSKP 240

Query: 241 SALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCTHH 277
           SALHGGAAGAAL+PQKSYIDANKLPFVLYSVGPFAFEPTC HH
Sbjct: 241 SALHGGAAGAALKPQKSYIDANKLPFVLYSVGPFAFEPTCPHH 274

BLAST of Lcy04g021380 vs. ExPASy TrEMBL
Match: A0A6J1EEB1 (non-classical arabinogalactan protein 31-like OS=Cucurbita moschata OX=3662 GN=LOC111433343 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.0e-110
Identity = 216/274 (78.83%), Postives = 232/274 (84.67%), Query Frame = 0

Query: 4   SLSLLFFFAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPT 63
           S   L FFAIFA++ ADD  A ETLA PPHHYAA+PPA     HHHHHH+PTPAPVAPPT
Sbjct: 8   SFFFLLFFAIFAVSTADDTTAAETLAAPPHHYAAVPPA-----HHHHHHSPTPAPVAPPT 67

Query: 64  HLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPANAPSHHLPPTHPPAHSPAPA-HHHR 123
           HLP+    HPPAQPPSH HHHH H+H QPPVHP    P +HL    PPAH+PAP  HHH 
Sbjct: 68  HLPL----HPPAQPPSHHHHHH-HIHSQPPVHP----PHYHL----PPAHAPAPGHHHHH 127

Query: 124 DVLPLPPPAHSPAPIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLV 183
           DV PLPPP HSPAP++PPK RL RSFISVQGVVYCKSCKYAGVDTL+GAT VAGATVKL+
Sbjct: 128 DVHPLPPPTHSPAPLHPPKPRLLRSFISVQGVVYCKSCKYAGVDTLLGATTVAGATVKLI 187

Query: 184 CQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAG 243
           CQNTKYPLVQTATTDKNGYFFITAPKA+TSYAFHKCKV+L SSP+ SCSKPSALHGGAAG
Sbjct: 188 CQNTKYPLVQTATTDKNGYFFITAPKAVTSYAFHKCKVVLGSSPTPSCSKPSALHGGAAG 247

Query: 244 AALRPQKSYIDANKLPFVLYSVGPFAFEPTCTHH 277
           A LRPQKSYIDANKLP+VLYSVGPFAFEPTC HH
Sbjct: 248 APLRPQKSYIDANKLPYVLYSVGPFAFEPTCAHH 263

BLAST of Lcy04g021380 vs. ExPASy TrEMBL
Match: A0A6J1KXA6 (non-classical arabinogalactan protein 31-like OS=Cucurbita maxima OX=3661 GN=LOC111497200 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.8e-109
Identity = 226/314 (71.97%), Postives = 242/314 (77.07%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPA--HHHHHHHAP 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPA  HHHHHHH P
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLAESPHHYAAVPPAQPPAHHHHHHHHHTP 60

Query: 61  TPAPVAPPTHLPIHP------------------------------PVHPPAQPPSHRHHH 120
            PAPV+PPTH+P+HP                              PVHPPAQPPSH H  
Sbjct: 61  KPAPVSPPTHVPVHPPAQPPSHHHHHIHSQPPVHPPVHNAPPTHLPVHPPAQPPSHHH-- 120

Query: 121 HAHVHGQPPVHPPA-NAPSHHLPPTHPPAHSPAPAH-HHRDVLPLPPPAHSPAPIYPPKS 180
             +VH QPPVHPP  NAP HHLPPT      PAP H HHRDV PL PPAHSP+P+YPPK 
Sbjct: 121 --NVHAQPPVHPPVNNAPPHHLPPT----KMPAPGHEHHRDVRPLLPPAHSPSPMYPPKP 180

Query: 181 RLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYF 240
           RL RSFISVQGVVYCKSCKYAGVDTL GATPVAGA+VKL+CQNTKYPLVQTATTDKNGYF
Sbjct: 181 RLLRSFISVQGVVYCKSCKYAGVDTLNGATPVAGASVKLICQNTKYPLVQTATTDKNGYF 240

Query: 241 FITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPFVLY 277
           FI APKAITSYAFHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPFVLY
Sbjct: 241 FINAPKAITSYAFHKCKVLLGSSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPFVLY 300

BLAST of Lcy04g021380 vs. NCBI nr
Match: XP_038881349.1 (non-classical arabinogalactan protein 31-like [Benincasa hispida])

HSP 1 Score: 436.4 bits (1121), Expect = 1.8e-118
Identity = 232/282 (82.27%), Postives = 243/282 (86.17%), Query Frame = 0

Query: 1   MDFSLS----LLFFFAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHHHHHHAPTP 60
           M F+LS     L FFA+FA +AAD   A ETL +P H YAA+PPA      HHHHHAPTP
Sbjct: 1   MAFTLSSSFFFLLFFAVFAFSAADHITAPETLPVPHHPYAAVPPA------HHHHHAPTP 60

Query: 61  APVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPANAPSHHLPPTHPPAHSPA 120
           APV  PTHLPIH PVHPPAQPPS RHHHH HVHGQPPVHPPANAPSHHLPPTHPPAHSPA
Sbjct: 61  APVPSPTHLPIHHPVHPPAQPPS-RHHHH-HVHGQPPVHPPANAPSHHLPPTHPPAHSPA 120

Query: 121 PAH--HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPV 180
           P H  HH D+ PLPPP HSPAPIYPPK RL RSFISVQGVVYCKSCKYAG DTL+GATPV
Sbjct: 121 PGHHRHHHDIRPLPPPTHSPAPIYPPKPRLVRSFISVQGVVYCKSCKYAGADTLLGATPV 180

Query: 181 AGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPS 240
           AGA+VKL+CQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKV+L SSPS SC+KPS
Sbjct: 181 AGASVKLICQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVILGSSPSPSCAKPS 240

Query: 241 ALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCTHH 277
           ALHGGAAGA LRPQKSYIDANKLPFVLYSVGPFAFEPTC HH
Sbjct: 241 ALHGGAAGAPLRPQKSYIDANKLPFVLYSVGPFAFEPTCPHH 274

BLAST of Lcy04g021380 vs. NCBI nr
Match: KAG6594600.1 (Non-classical arabinogalactan protein 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 420.6 bits (1080), Expect = 1.0e-113
Identity = 231/302 (76.49%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHH-HHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPAHHH HHHHAP 
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLATSPHHYAAVPPAQPPAHHHYHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTH----- 120
           PAPV+PPTHL    PVHPPAQPPSH HHHH HVH QPPVHPP  NAPSHHLPPTH     
Sbjct: 61  PAPVSPPTHL----PVHPPAQPPSHNHHHH-HVHSQPPVHPPVHNAPSHHLPPTHLPVHP 120

Query: 121 --------------PPAHSPAPAH-HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGV 180
                         PP  +PAP H HHRDV PL PPAHSP+P+YPPK RL RSFISVQGV
Sbjct: 121 PVHPPVNNAPSHHLPPTKAPAPGHEHHRDVRPLLPPAHSPSPMYPPKPRLLRSFISVQGV 180

Query: 181 VYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYA 240
           VYCKSCKYAGVDTL GATPVAGA+VKL+CQNTK+PLVQTATTD NGYFFI APKAITSYA
Sbjct: 181 VYCKSCKYAGVDTLNGATPVAGASVKLICQNTKHPLVQTATTDNNGYFFINAPKAITSYA 240

Query: 241 FHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCT 277
           FHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPFVLYSVGPFAFEPTC 
Sbjct: 241 FHKCKVLLGSSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPFVLYSVGPFAFEPTCP 297

BLAST of Lcy04g021380 vs. NCBI nr
Match: XP_022926436.1 (non-classical arabinogalactan protein 30-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 419.9 bits (1078), Expect = 1.8e-113
Identity = 231/302 (76.49%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPAHHH-HHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPAHHH HHHHAP 
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLATSPHHYAAVPPAQPPAHHHYHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTHPPAHS 120
           PAPV+PPTHL    PVHPPAQPPSH HHHH HVH QPPVHPP  NAPSHHLPPTH P H 
Sbjct: 61  PAPVSPPTHL----PVHPPAQPPSHNHHHH-HVHSQPPVHPPVHNAPSHHLPPTHLPVHP 120

Query: 121 P-------APAH-------------HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGV 180
           P       AP+H             HHRDV PL PPAHSP+P+YPPK RL RSFISVQGV
Sbjct: 121 PVHPPVNNAPSHHLPPTKALAPGHEHHRDVRPLLPPAHSPSPMYPPKPRLLRSFISVQGV 180

Query: 181 VYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYA 240
           VYCKSCKYAGVDTL GATPVAGA+VKL+CQNTK+PLVQTATTD NGYFFI APKAITSYA
Sbjct: 181 VYCKSCKYAGVDTLNGATPVAGASVKLICQNTKHPLVQTATTDNNGYFFINAPKAITSYA 240

Query: 241 FHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCT 277
           FHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPFVLYSVGPFAFEPTC 
Sbjct: 241 FHKCKVLLGSSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPFVLYSVGPFAFEPTCP 297

BLAST of Lcy04g021380 vs. NCBI nr
Match: KAG7026570.1 (Non-classical arabinogalactan protein 31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.8 bits (1070), Expect = 1.5e-112
Identity = 230/302 (76.16%), Postives = 245/302 (81.13%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPA-HHHHHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PPA HHHHHHHAP 
Sbjct: 1   MDFALSKSFFFLLSFAIFAVSTAEDVTAAETLATSPHHYAAVPPAQPPAHHHHHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTHPPAHS 120
           PAPV+PPTHL    PVHPPAQPPSH HHHH HVH QPPV PP  NAPSHHLPPTH P H 
Sbjct: 61  PAPVSPPTHL----PVHPPAQPPSHNHHHH-HVHSQPPVQPPVHNAPSHHLPPTHLPVHP 120

Query: 121 P-------APAH-------------HHRDVLPLPPPAHSPAPIYPPKSRLPRSFISVQGV 180
           P       AP+H             HHRDV PL PPAHSP+P+YPPK RL RSFISVQGV
Sbjct: 121 PVHPPVNNAPSHHLPPTKALAPGHEHHRDVRPLLPPAHSPSPMYPPKPRLLRSFISVQGV 180

Query: 181 VYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYA 240
           VYCKSCKYAGVDTL GATPVAGA+VKL+CQNTK+PLVQTATTD NGYFFI APKAITSYA
Sbjct: 181 VYCKSCKYAGVDTLNGATPVAGASVKLICQNTKHPLVQTATTDNNGYFFINAPKAITSYA 240

Query: 241 FHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPFVLYSVGPFAFEPTCT 277
           FHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPFVLYSVGPFAFEPTC 
Sbjct: 241 FHKCKVLLGSSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPFVLYSVGPFAFEPTCP 297

BLAST of Lcy04g021380 vs. NCBI nr
Match: XP_023517088.1 (non-classical arabinogalactan protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.1 bits (1063), Expect = 9.6e-112
Identity = 228/317 (71.92%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 1   MDFSLSLLFF----FAIFAIAAADDFAAEETLALPPHHYAAMPPAKPPA-HHHHHHHAPT 60
           MDF+LS  FF    FAIFA++ A+D  A ETLA  PHHYAA+PPA+PP  HHHHHHHAP 
Sbjct: 1   MDFALSKPFFLLLSFAIFAVSTAEDVTAAETLAASPHHYAAVPPAQPPTHHHHHHHHAPK 60

Query: 61  PAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPA-NAPSHHLPPTH----- 120
           PAPV+PPTHL    PVH PAQPPSH HHHH HVH QPPVHPP  NAPSHHLPPTH     
Sbjct: 61  PAPVSPPTHL----PVHTPAQPPSHNHHHHHHVHSQPPVHPPVYNAPSHHLPPTHLPVHP 120

Query: 121 -----------------------------PPAHSPAPAH-HHRDVLPLPPPAHSPAPIYP 180
                                        PP  +PAP H HHRDV PL PPAHSP+P+YP
Sbjct: 121 PAQPPSHHHNVHAQPPVHPPVNNAPSHHLPPTKAPAPGHEHHRDVRPLLPPAHSPSPMYP 180

Query: 181 PKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKN 240
           PK RL RSFISV+GVVYCKSCKYAGVDTL GATPVAGA+VKL+CQNTK+PLVQTATTD N
Sbjct: 181 PKPRLLRSFISVEGVVYCKSCKYAGVDTLNGATPVAGASVKLICQNTKHPLVQTATTDNN 240

Query: 241 GYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQKSYIDANKLPF 277
           GYFFI APKAITSYAFHKCKVLL SSP A+CSKPSALHGGAAGAAL+PQKSYIDANKLPF
Sbjct: 241 GYFFINAPKAITSYAFHKCKVLLASSPVAACSKPSALHGGAAGAALKPQKSYIDANKLPF 300

BLAST of Lcy04g021380 vs. TAIR 10
Match: AT1G28290.2 (arabinogalactan protein 31 )

HSP 1 Score: 172.2 bits (435), Expect = 5.9e-43
Identity = 119/258 (46.12%), Postives = 148/258 (57.36%), Query Frame = 0

Query: 31  PPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHG 90
           PP      PPAKPP       + PT APV PPT  P+ PPV PPA+PP            
Sbjct: 74  PPVKAPVSPPAKPPV--KPPVYPPTKAPVKPPTKPPVKPPVSPPAKPPV----------- 133

Query: 91  QPPVHPPANAPSHHLPPTHPPAHSPA-----PAHHHRDVLPLPPPAHSPA------PIYP 150
           +PPV+PP  AP    PPT PP   P      P  +     P+ PP   P       P+YP
Sbjct: 134 KPPVYPPTKAPVK--PPTKPPVKPPVYPPTKPPVYPPTKAPVKPPVSPPTKPPVTPPVYP 193

Query: 151 PKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKN 210
           PK    RS ++V+G VYCKSCKYA  +TL+GA P+ GATVKLVC++ K    +T TTDKN
Sbjct: 194 PK--FNRSLVAVRGTVYCKSCKYAAFNTLLGAKPIEGATVKLVCKSKKNITAET-TTDKN 253

Query: 211 GYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQ----KSYIDAN 270
           GYF + APK +T++ F  C+V LV S    CSK S L GG  GA L+P+    KS +  N
Sbjct: 254 GYFLLLAPKTVTNFGFRGCRVYLVKSKDYKCSKVSKLFGGDVGAELKPEKKLGKSTVVVN 313

Query: 271 KLPFVLYSVGPFAFEPTC 274
           KL + L++VGPFAF P+C
Sbjct: 314 KLVYGLFNVGPFAFNPSC 313

BLAST of Lcy04g021380 vs. TAIR 10
Match: AT1G28290.1 (arabinogalactan protein 31 )

HSP 1 Score: 166.4 bits (420), Expect = 3.3e-41
Identity = 114/247 (46.15%), Postives = 145/247 (58.70%), Query Frame = 0

Query: 31  PPHHYAAMPPAKPPAHHHHHHHAPTPAPVAPPTHLPIHPPVHPPAQPPSHRHHHHAHVHG 90
           PP      PP KPP       + PT APV PPT  P+ PPV+PP + P            
Sbjct: 126 PPTKAPVKPPTKPPV--KPPVYPPTKAPVKPPTKPPVKPPVYPPTKAP-------VKPPT 185

Query: 91  QPPVHPPANAPSHHLPPTHPPAHSPAPAHHHRDVLPLPPPAHSPAPIYPPKSRLPRSFIS 150
           +PPV PP + P+   PP  PP + P  A     V P   P  +P P+YPPK    RS ++
Sbjct: 186 KPPVKPPVSPPAK--PPVKPPVYPPTKAPVKPPVSPPTKPPVTP-PVYPPK--FNRSLVA 245

Query: 151 VQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPLVQTATTDKNGYFFITAPKAI 210
           V+G VYCKSCKYA  +TL+GA P+ GATVKLVC++ K    +T TTDKNGYF + APK +
Sbjct: 246 VRGTVYCKSCKYAAFNTLLGAKPIEGATVKLVCKSKKNITAET-TTDKNGYFLLLAPKTV 305

Query: 211 TSYAFHKCKVLLVSSPSASCSKPSALHGGAAGAALRPQ----KSYIDANKLPFVLYSVGP 270
           T++ F  C+V LV S    CSK S L GG  GA L+P+    KS +  NKL + L++VGP
Sbjct: 306 TNFGFRGCRVYLVKSKDYKCSKVSKLFGGDVGAELKPEKKLGKSTVVVNKLVYGLFNVGP 357

Query: 271 FAFEPTC 274
           FAF P+C
Sbjct: 366 FAFNPSC 357

BLAST of Lcy04g021380 vs. TAIR 10
Match: AT2G34700.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-35
Identity = 73/147 (49.66%), Postives = 102/147 (69.39%), Query Frame = 0

Query: 133 SPAPIYPPKS--RLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQNTKYPL 192
           S +P+ PP S  ++ R  ++V+G+VYCKSCKY+GVDTL+ A+P+ GATVKL C NTK  +
Sbjct: 28  SASPMTPPSSPAKMSRRLVAVEGMVYCKSCKYSGVDTLLEASPLQGATVKLACNNTKRGV 87

Query: 193 VQTATTDKNGYFFITAPKAITSYAFHKCKVLLV----SSPSASCSKPSALHGGAAGAALR 252
                TDKNGYFF+ APK +T+YAFH C+        ++ + +C+ PS L+ G  GA L+
Sbjct: 88  TMETKTDKNGYFFMLAPKKLTTYAFHTCRAWPTNPGPTTATMTCTVPSKLNNGITGAMLK 147

Query: 253 PQKSYIDANKLPFVLYSVGPFAFEPTC 274
           P K+ I+  +  +VL+SVGPFAFEP C
Sbjct: 148 PSKT-INIGEHDYVLFSVGPFAFEPAC 173

BLAST of Lcy04g021380 vs. TAIR 10
Match: AT2G33790.1 (arabinogalactan protein 30 )

HSP 1 Score: 138.3 bits (347), Expect = 9.5e-33
Identity = 96/218 (44.04%), Postives = 124/218 (56.88%), Query Frame = 0

Query: 62  PTHLPIHPPVHPPAQPPSHRHHHHAHVHGQPPVHPPANAPSHHLPPTHPPAHSPAPAHHH 121
           P HLP+ PP+  P  PP       A    + P +PPA AP     PT PPA +P      
Sbjct: 40  PQHLPL-PPIKLPTLPP-------AKAPIKLPAYPPAKAPIK--LPTLPPAKAPIK---- 99

Query: 122 RDVLPLPPPAHSPA--PIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATV 181
              LP  PP   P   P+YPPK    ++ ++V+GVVYCK+CKYAGV+ + GA PV  A V
Sbjct: 100 ---LPTLPPIKPPVLPPVYPPKYN--KTLVAVRGVVYCKACKYAGVNNVQGAKPVKDAVV 159

Query: 182 KLVCQNTKYPLVQTATTDKNGYFFITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHGG 241
           +LVC+N K  + +T  TDKNGYF + APK +T+Y    C+  LV SP   CSK S+LH G
Sbjct: 160 RLVCKNKKNSISET-KTDKNGYFMLLAPKTVTNYDIKGCRAFLVKSPDTKCSKVSSLHDG 219

Query: 242 AAGAALRPQK----SYIDANKLPFVLYSVGPFAFEPTC 274
             G+ L+P      S        + +Y+VGPFAFEPTC
Sbjct: 220 GKGSVLKPVLKPGFSSTIMRWFKYSVYNVGPFAFEPTC 237

BLAST of Lcy04g021380 vs. TAIR 10
Match: AT5G05500.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.2e-12
Identity = 47/153 (30.72%), Postives = 73/153 (47.71%), Query Frame = 0

Query: 126 PLPPPAHSPAPIYPPKSRLPRSFISVQGVVYCKSCKYAGVDTLIGATPVAGATVKLVCQN 185
           P PPP   P   YP    +     +V+G+VYC+SC   G  +L GA  +AGA + ++C+N
Sbjct: 26  PPPPP---PTTAYPAVKTVE---AAVEGMVYCQSCDKFGSWSLAGAEAIAGAKISIICKN 85

Query: 186 TKYPL--VQTATTDKNGYFF-----ITAPKAITSYAFHKCKVLLVSSPSASCSKPSALHG 245
            +  +   +   TD  G+F+              +  H C+  LVSSP   C+  S ++ 
Sbjct: 86  HRQQVSFYKVFRTDSYGHFYGELKGFKMTPHFLDHPLHSCRAKLVSSPREDCNLFSNINN 145

Query: 246 GAAGAALRPQKSYIDANKLPFVLYSVGPFAFEP 272
              GA LR ++  +       V+Y+ GP AF P
Sbjct: 146 ALDGAPLRYEEKRLKWTNYEAVIYAAGPLAFRP 172