Lcy04g021300 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g021300
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	heat stress transcription factor B-2a-like
Location	Chr04: 52600025 .. 52600944 (-)
RNA-Seq Expression	Lcy04g021300
Synteny	Lcy04g021300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAAGAAGAAGCTGGAAGTAAGAGGATTGTGTCATGGAATGCAGAGGGAAATGGATTCATAGTCTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGTATGTGATTCTCTCTTTCTCTCTCTTTCTCTTTTTGAGAATGTCCCCACCATTTGTTATTCTCTGAAACATCTTGAAGAAGAGTTATTCATTATAAAGTGGATTCAATTGGCCATTTTCCTGTTTTGGGATAATGCTTCAATTCTTCCTTAGGAATTGGATCTATAGGTATGTTTTTGTTTATGACAGTGTGGCAAATGAGAGTTTCGAGCGTACTGCTAAATTTAAAGGGTTGGATTGATAGAGTGATATATTTGATCATTTCTATATGTTCTTAACATCGTTTTCCGAGTCGGGTATCAATCTAAATATTGTAATATTGGAGTCAGGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCAGGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGGTCAGTGCATTGAGAAATAG

mRNA sequence

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAAGAAGAAGCTGGAAGTAAGAGGATTGTGTCATGGAATGCAGAGGGAAATGGATTCATAGTCTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCAGGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGGTCAGTGCATTGAGAAATAG

Coding sequence (CDS)

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAAGAAGAAGCTGGAAGTAAGAGGATTGTGTCATGGAATGCAGAGGGAAATGGATTCATAGTCTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCAGGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGGTCAGTGCATTGAGAAATAG

Protein sequence

MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKALHIRLLDCLGQCIEK

Homology

[Close]

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.6e-29
Identity = 73/205 (35.61%), Postives = 109/205 (53.17%), Query Frame = 0


Query: 7   SSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWS 66
           SS+   P PFL+KT++L+                    E++    ++SWN +G+ FIVW+
Sbjct: 15  SSQRSIPTPFLTKTFNLV--------------------EDSSIDDVISWNEDGSSFIVWN 74

Query: 67  PAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK 126
           P +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG +++L EI R+K
Sbjct: 75  PTDFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK 134

Query: 127 CEPSVFPAFLRSSQEGAATMAVDQEN-----------------------------GDHLL 183
              +       SS++   TM V   N                             G  + 
Sbjct: 135 ITTTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVE 194

[Close]

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-27
Identity = 64/136 (47.06%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSE 72
           P PFL+KTY L+++       A D               ++SWN +G+ F+VW PAEF+ 
Sbjct: 46  PTPFLTKTYQLVDD------PAVDD--------------VISWNDDGSTFVVWRPAEFAR 105

Query: 73  ILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEPSVF 132
            LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF ++ F+RG R++L EI R+K  P   
Sbjct: 106 DLLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPA- 160

Query: 133 PAFLRSSQEGAATMAV 149
           PA   ++   A  MA+
Sbjct: 166 PAATTAAVAAAIPMAL 160

[Close]

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot
Match: O81821 (Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 4.1e-27
Identity = 70/173 (40.46%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSEIL 74
           PFLSKTYD++                    ++  +  +VSW++  N F+VWS  EFS++L
Sbjct: 27  PFLSKTYDMV--------------------DDPLTNEVVSWSSGNNSFVVWSAPEFSKVL 86

Query: 75  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CEPSV 134
           LPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    + + 
Sbjct: 87  LPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQ 146

Query: 135 FPAFLRSSQEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 177
               ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot
Match: Q6VBB2 (Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2B PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.0e-27
Identity = 72/192 (37.50%), Postives = 101/192 (52.60%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIV 64
           M+      P PFL+KTYD++                    ++AG+   VSW+A  N F+V
Sbjct: 42  MEGLHDAGPPPFLTKTYDMV--------------------DDAGTDAAVSWSATSNSFVV 101

Query: 65  WSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVR 124
           W P  F+ +LLP++FKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG R +L  I R
Sbjct: 102 WDPHAFATVLLPRFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANENFLRGQRHLLKNIKR 161

Query: 125 KKCEPSVFPAFLRSSQEGAA----------TMAVDQENGDHLLLMEENKNLRRQKLELQA 184
           +K      P+   S+Q+                +D+   D  LLM E   LR+++   +A
Sbjct: 162 RKP-----PSHTASNQQSLGPYLEVGHFGYDAEIDRLKRDKQLLMAEVVKLRQEQQNTKA 205

Query: 185 QIAQFKALHIRL 187
            +   KA+  RL
Sbjct: 222 NL---KAMEDRL 205

[Close]

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 9.2e-27
Identity = 69/168 (41.07%), Postives = 98/168 (58.33%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSE 72
           PAPFL KTY+++                    +++ + +IVSW+A  N FIVW+ AEFS 
Sbjct: 21  PAPFLVKTYEMV--------------------DDSSTDQIVSWSANNNSFIVWNHAEFSR 80

Query: 73  ILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEPSVF 132
           +LLP YFKHNNFSSFIRQLNTYGF+K  P+RWEF ++ F +  + +L  I R+K      
Sbjct: 81  LLLPTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK------ 140

Query: 133 PAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFK 181
           P    S    ++T   DQE     +L E+   L R+K  ++A++ +FK
Sbjct: 141 PIHSHSHPPASST---DQERA---VLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL
Match: A0A6J1ED85 (heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433105 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.9e-92
Identity = 171/198 (86.36%), Postives = 188/198 (94.95%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEE--AGSKRIVSWNAE 60
           M+L+MQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E+++E+E   G  RIVSWNA+
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEENGGGNRIVSWNAD 60

Query: 61  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 120
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR+M
Sbjct: 61  GSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRRM 120

Query: 121 LGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 180
           L EIVRKKCEPSVFPA+LRSS EGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAYLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 180

Query: 181 FKALHIRLLDCLGQCIEK 197
           FKALHIRLLDC+G+ IEK
Sbjct: 181 FKALHIRLLDCVGRRIEK 198

[Close]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL
Match: A0A6J1EFX0 (heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC111433815 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.4e-91
Identity = 172/192 (89.58%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIV 64
           MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQE+E+E+EEE    RIVSWNAEGNGFIV
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEE-ENRIVSWNAEGNGFIV 60

Query: 65  WSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVR 124
           WSPAEFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRGN+QMLGEIVR
Sbjct: 61  WSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVR 120

Query: 125 KKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKALHI 184
           KKCEPSVFPAFLRSS EGA TMAV Q NGDHL LMEENKNLRR+KLELQA+IA+FKALHI
Sbjct: 121 KKCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHI 180

Query: 185 RLLDCLGQCIEK 197
           RLLDCL QCI+K
Sbjct: 181 RLLDCLDQCIKK 191

[Close]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL
Match: A0A5D3CQ35 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007440 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 173/194 (89.18%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQ--EQEQEQEEEAGSKRIVSWNAEGNGF 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQ  E E+E+EEE G  RIVSWNAEG+GF
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEEEEEEENGGNRIVSWNAEGSGF 60

Query: 65  IVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEI 124
           IVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R ML EI
Sbjct: 61  IVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHMLVEI 120

Query: 125 VRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKAL 184
           VRKKCEPSVFPAFLRSS EG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQFKAL
Sbjct: 121 VRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQFKAL 180

Query: 185 HIRLLDCLGQCIEK 197
           HIRLLDCLGQCIEK
Sbjct: 181 HIRLLDCLGQCIEK 194

[Close]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL
Match: A0A5A7UBZ5 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003020 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 173/198 (87.37%), Postives = 181/198 (91.41%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQ------EQEQEQEEEAGSKRIVSWNAE 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQ      E+E+E+EEE G  RIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 65  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 124
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 125 LGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184
           L EIVRKKCEPSVFPAFLRSS EG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 185 FKALHIRLLDCLGQCIEK 197
           FKALHIRLLDCLGQCIEK
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

[Close]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL
Match: A0A1S3AZS2 (heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC103484524 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 173/198 (87.37%), Postives = 181/198 (91.41%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQ------EQEQEQEEEAGSKRIVSWNAE 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQ      E+E+E+EEE G  RIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 65  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 124
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 125 LGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184
           L EIVRKKCEPSVFPAFLRSS EG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 185 FKALHIRLLDCLGQCIEK 197
           FKALHIRLLDCLGQCIEK
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

[Close]

BLAST of Lcy04g021300 vs. NCBI nr
Match: XP_022925809.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 347.8 bits (891), Expect = 6.0e-92
Identity = 171/198 (86.36%), Postives = 188/198 (94.95%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEE--AGSKRIVSWNAE 60
           M+L+MQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E+++E+E   G  RIVSWNA+
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEENGGGNRIVSWNAD 60

Query: 61  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 120
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR+M
Sbjct: 61  GSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRRM 120

Query: 121 LGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 180
           L EIVRKKCEPSVFPA+LRSS EGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAYLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 180

Query: 181 FKALHIRLLDCLGQCIEK 197
           FKALHIRLLDC+G+ IEK
Sbjct: 181 FKALHIRLLDCVGRRIEK 198

[Close]

BLAST of Lcy04g021300 vs. NCBI nr
Match: XP_023543806.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 172/201 (85.57%), Postives = 186/201 (92.54%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEE-----AGSKRIVSW 60
           M+LVMQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E+++E+E      G  RIVSW
Sbjct: 1   MSLVMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEEAEENGGGNRIVSW 60

Query: 61  NAEGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN 120
           NA+G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN
Sbjct: 61  NADGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN 120

Query: 121 RQMLGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQ 180
           R ML EIVRKKCEPSVFPAFLRSS EGAATM VDQENGDHLLLMEENKNLRRQKLELQAQ
Sbjct: 121 RHMLVEIVRKKCEPSVFPAFLRSSHEGAATMGVDQENGDHLLLMEENKNLRRQKLELQAQ 180

Query: 181 IAQFKALHIRLLDCLGQCIEK 197
           IAQFKALHIRLLDC+G+ IEK
Sbjct: 181 IAQFKALHIRLLDCVGRRIEK 201

[Close]

BLAST of Lcy04g021300 vs. NCBI nr
Match: XP_022926806.1 (heat stress transcription factor B-2a-like [Cucurbita moschata])

HSP 1 Score: 345.5 bits (885), Expect = 3.0e-91
Identity = 172/192 (89.58%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIV 64
           MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQE+E+E+EEE    RIVSWNAEGNGFIV
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEE-ENRIVSWNAEGNGFIV 60

Query: 65  WSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVR 124
           WSPAEFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRGN+QMLGEIVR
Sbjct: 61  WSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVR 120

Query: 125 KKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKALHI 184
           KKCEPSVFPAFLRSS EGA TMAV Q NGDHL LMEENKNLRR+KLELQA+IA+FKALHI
Sbjct: 121 KKCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHI 180

Query: 185 RLLDCLGQCIEK 197
           RLLDCL QCI+K
Sbjct: 181 RLLDCLDQCIKK 191

[Close]

BLAST of Lcy04g021300 vs. NCBI nr
Match: TYK13134.1 (heat stress transcription factor B-2a-like [Cucumis melo var. makuwa])

HSP 1 Score: 345.1 bits (884), Expect = 3.9e-91
Identity = 173/194 (89.18%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQ--EQEQEQEEEAGSKRIVSWNAEGNGF 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQ  E E+E+EEE G  RIVSWNAEG+GF
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEEEEEEENGGNRIVSWNAEGSGF 60

Query: 65  IVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEI 124
           IVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R ML EI
Sbjct: 61  IVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHMLVEI 120

Query: 125 VRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKAL 184
           VRKKCEPSVFPAFLRSS EG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQFKAL
Sbjct: 121 VRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQFKAL 180

Query: 185 HIRLLDCLGQCIEK 197
           HIRLLDCLGQCIEK
Sbjct: 181 HIRLLDCLGQCIEK 194

[Close]

BLAST of Lcy04g021300 vs. NCBI nr
Match: XP_008439862.1 (PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo] >KAA0052690.1 heat stress transcription factor B-2a-like [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 6.7e-91
Identity = 173/198 (87.37%), Postives = 181/198 (91.41%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQ------EQEQEQEEEAGSKRIVSWNAE 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQ      E+E+E+EEE G  RIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 65  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 124
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 125 LGEIVRKKCEPSVFPAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184
           L EIVRKKCEPSVFPAFLRSS EG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 185 FKALHIRLLDCLGQCIEK 197
           FKALHIRLLDCLGQCIEK
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

[Close]

BLAST of Lcy04g021300 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 130.2 bits (326), Expect = 1.8e-30
Identity = 73/205 (35.61%), Postives = 109/205 (53.17%), Query Frame = 0


Query: 7   SSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWS 66
           SS+   P PFL+KT++L+                    E++    ++SWN +G+ FIVW+
Sbjct: 15  SSQRSIPTPFLTKTFNLV--------------------EDSSIDDVISWNEDGSSFIVWN 74

Query: 67  PAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK 126
           P +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG +++L EI R+K
Sbjct: 75  PTDFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK 134

Query: 127 CEPSVFPAFLRSSQEGAATMAVDQEN-----------------------------GDHLL 183
              +       SS++   TM V   N                             G  + 
Sbjct: 135 ITTTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVE 194

[Close]

BLAST of Lcy04g021300 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 70/173 (40.46%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSEIL 74
           PFLSKTYD++                    ++  +  +VSW++  N F+VWS  EFS++L
Sbjct: 27  PFLSKTYDMV--------------------DDPLTNEVVSWSSGNNSFVVWSAPEFSKVL 86

Query: 75  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CEPSV 134
           LPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    + + 
Sbjct: 87  LPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQ 146

Query: 135 FPAFLRSSQEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 177
               ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lcy04g021300 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 70/173 (40.46%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSEIL 74
           PFLSKTYD++                    ++  +  +VSW++  N F+VWS  EFS++L
Sbjct: 27  PFLSKTYDMV--------------------DDPLTNEVVSWSSGNNSFVVWSAPEFSKVL 86

Query: 75  LPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CEPSV 134
           LPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    + + 
Sbjct: 87  LPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQ 146

Query: 135 FPAFLRSSQEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 177
               ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lcy04g021300 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 121.7 bits (304), Expect = 6.5e-28
Identity = 69/168 (41.07%), Postives = 98/168 (58.33%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSE 72
           PAPFL KTY+++                    +++ + +IVSW+A  N FIVW+ AEFS 
Sbjct: 21  PAPFLVKTYEMV--------------------DDSSTDQIVSWSANNNSFIVWNHAEFSR 80

Query: 73  ILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEPSVF 132
           +LLP YFKHNNFSSFIRQLNTYGF+K  P+RWEF ++ F +  + +L  I R+K      
Sbjct: 81  LLLPTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK------ 140

Query: 133 PAFLRSSQEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFK 181
           P    S    ++T   DQE     +L E+   L R+K  ++A++ +FK
Sbjct: 141 PIHSHSHPPASST---DQERA---VLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of Lcy04g021300 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-27
Identity = 77/197 (39.09%), Postives = 103/197 (52.28%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEEEAGSKRIVSWNAEGNGFIVWSPAEFSE 72
           P PFLSKTYD++                    E+  +  IVSW+   N FIVW P EFS 
Sbjct: 50  PPPFLSKTYDMV--------------------EDPATDAIVSWSPTNNSFIVWDPPEFSR 109

Query: 73  ILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKC----- 132
            LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG + +L +I R+K      
Sbjct: 110 DLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHG 169

Query: 133 EPSVFPAFLRSSQEGAATMA----------------VDQENGDHLLLMEENKNLRRQKLE 189
             S  P   + SQ G  +MA                V+Q   D  +LM+E   LR+Q+  
Sbjct: 170 SSSSNPQSQQLSQ-GQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQT 224

The following BLAST results are available for this feature:

BLAST of Lcy04g021300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCW4	2.6e-29	35.61	Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ...	[more]
Q6Z9C8	3.2e-27	47.06	Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
O81821	4.1e-27	40.46	Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B ...	[more]
Q6VBB2	7.0e-27	37.50	Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q94BZ5	9.2e-27	41.07	Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE...	[more]

BLAST of Lcy04g021300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ED85	2.9e-92	86.36	heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1EFX0	1.4e-91	89.58	heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5D3CQ35	1.9e-91	89.18	Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5A7UBZ5	3.2e-91	87.37	Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3AZS2	3.2e-91	87.37	heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC1034845...	[more]

BLAST of Lcy04g021300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925809.1	6.0e-92	86.36	heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata]	[more]
XP_023543806.1	1.8e-91	85.57	heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pep...	[more]
XP_022926806.1	3.0e-91	89.58	heat stress transcription factor B-2a-like [Cucurbita moschata]	[more]
TYK13134.1	3.9e-91	89.18	heat stress transcription factor B-2a-like [Cucumis melo var. makuwa]	[more]
XP_008439862.1	6.7e-91	87.37	PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo] >KAA0052690...	[more]

BLAST of Lcy04g021300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62020.1	1.8e-30	35.61	heat shock transcription factor B2A	[more]
AT5G16820.1	2.9e-28	40.46	heat shock factor 3	[more]
AT5G16820.2	2.9e-28	40.46	heat shock factor 3	[more]
AT4G13980.1	6.5e-28	41.07	winged-helix DNA-binding transcription factor family protein	[more]
AT4G17750.1	2.5e-27	39.09	heat shock factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 155..182
None	No IPR available	PANTHER	PTHR10015:SF308	HEAT STRESS TRANSCRIPTION FACTOR B-2A-LIKE	coord: 6..195
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 74..86 score: 81.98 coord: 36..59 score: 16.31 coord: 87..99 score: 74.51
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 12..125 e-value: 5.4E-37 score: 138.9
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 16..125 e-value: 3.1E-28 score: 98.2
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 8..126 e-value: 1.0E-30 score: 108.2
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 6..195
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 48..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g021300.1	Lcy04g021300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding