Lcy04g015300 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g015300
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionformamidase-like isoform X2
LocationChr04: 47607130 .. 47611228 (+)
RNA-Seq ExpressionLcy04g015300
SyntenyLcy04g015300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTCCACTGTTTGAGTGTCATCAGATATCTTCAGAGACTCTGTGAAACTTCCAAAAGAAGAAGGATATGGGTGATTATGGTCCACGGTTAGTGGTGCCTATAGATGTGAAGAAGAAGCCATGCCAACAGAAACTTCCACTTCACAACCGGTGGCACCCGGAGATACCGCCGGTGGCGGAGGTCAAAGTGGATGAGTTCTTCAGAGTTGAAATGGTGGATTTCAGCGGCAGAGGAATCACCAATGACTACTCTGCTGAGGACATTAAACATGCTGATACCTCTGTTGTAAGCCCTCTCTCTCACACAAATCTTTGCCTCGTCCCTGAGTTTTGAATTGGAAAAAATCACAGCCTAGCTAGTTTACAGAACAAAAAGAAGGGGCGATATTCTCAACTTAGCCGTCCGTTTGGAAATTGAAAGATACCATTCTTTGGTTGTCAACATCCTTAATGGACAAAAAAGACAAATTAGTTTCTTGTCCTTTGTTTGATCTCTTATCTTTCTGTGTACTTTTCAACAATTGCTGCTACTTAGATTAGAAGAGACTGTGTGCAATAGAAGAACCTAGCAGAAGAACCCAGCTCCTGACCACGTTAGGCGTCTAGTTTCTGTCTACTTCTTGTCAAAATTCATGGCTCCTTCTACTCCAAGACTTGTTGTTCCGATAGACTTGAAAAAGAAGCCATGGGATCAAAACTTGCCTCTTCACAACCGCTGGCACCCACTTATACCCCCAGTTGCTGAGGTTCACACGGATGAGTTTTTCAGGGTTGAGATGGTGGACTGGACTGGAGGAGTTATTGGAGATAATGACTCAGCCAGTGATGTACAGTTAATTGACCTTTCAATTGTAAGTATGAATATTAACCTTATCCAATCTTCTAGGAGATAAAAATCTAGAAATCATTGTCCTTTTGAGCCCCTAATCAGCCTGATAATTGGTAAAGCTTTTACTCAGTACAGAGACATGGTTAACTACAATGATCTAGAAAACATTGAATCTGACTTTGAATGAACAGTAATCTCCAAAGACGATAGATTTTAGTGCTAATAATGGGCTGTTAATATTACGTTTTTTCACTTTTTCTAAAATGTCTAGACACATTATCTGAGTGGTCCAATTAGAGTTGTGGACAAGGATGGGGTTCCAGCCAAGCCAGGGGATTTACTCGTAGTCGAAATCTGCAACTTGGGTCCTCTCCCAGGAGATGAATGGGGTTATACTGCAACATTTGACAGAGAAAATGGAGGTGGTTTTCTTACTGACCATTTTCCTTGTGCAACCAAAGCTATTTGGTATTTTGAAGGAATATATGCATACTCTCCTCAAATCCCAGGTAAATGCTCCAACCTGAAACAATGCTTCCAATAAGAATGGTAGCAATAAATGCCTTAACCCGTATTCGCCTTCCAGGAGTTCGATTCCCAGGTTTAACTCACCCTGGAGTGATTGGGACTGCACCATCGGCAGAACTTCTGAATATTTGGAATGAAAGGGAAAGGCACGTTCAAGAAAATGGCCTTCAAACTCTAAAACTTAGTGAGGTTTTGCATTCACGGCCATTGGCAAACCTACCAACAGCTAAAGGTTGCGCTCTTGGAAAGGTATCATCGTAAACTACCATCTTTCTTTGCATAAAGTGATAAAATTGCAATAGCTTTGGTTAACACCTTCCAAATTCTACAAACAAAATAATTGCGAGGCTGATCTTTCTCCTATTCAACTGAAGTGTAGAATGTAGTTTTCTAATATTCGATGCTCTATTGCTTCAAACTAGATCAAAGAGGGCACAGAAGAATGGGAAAGAATTGCCAATGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGAAACTGTGACATCAAGAACCTCAGTAGAGGTTCAAAAGTATACCTTCCAGTATTCGTGGAAGGGGCAAACTTGAGCACAGGTGACATGCACTTCTCCCAAGGAGATGGTGAAGTCTCATTTTGTGGAGCAATTGAAATGAGTGGTTTTCTAGAGCTCAAGTAAATTTCTCAGCTTGGCTTCTCAATCTCTTCAGATCCTTTTCATTTGTTTCTACTTCAGCAATTAATTATATAGCAGAAGTGATAGATAAAGAAAAGGAATCAGTCGGTCCATACAAATATTACTTGTTATCTTCTTGTTCAGGTGCGATATTATAAGGGGCGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTAAACCCAATCTTCGAGATAGGTCCAGTTGAACCAAGATTCTCAGAATGGCTGGTTTTTGAAGGCATCAGCGTGGATGAAAGTGGGAGGCAGCATTTTCTTGACGCGAGTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATCGACTATCTCCACAAATTTGGGTACTCAAAGGAACAGGTCAAATAACTTTCTTCGGTTGCCATCCATCTTATTTATTACCAGTTCCAGAACTCACACGAGCAAAATCACAAACACCTGAGATGCACACAAAAACTAAAAACTAATTCCAGTTCAATACGAACATTGAATATAAGATGTGTGAAATTTCAGGCTTATCTGCTGCTGTCGTGCTGCCCATGCGAAGGAAGGATCTCTGGGATTGTTGATTCACCAAATGCTGTGGCTACCTTGGCGATCCCCACCGCCATTTTCGACCAGGTAGCTTCCTCCTTCCAACTGGTTCGATTCCATCCAGTCGCATTACTTATGTCTAATTTCCTTCGCATTGTTAAACCACTAAGCCTTGGGCTGACAAATCCCTGGCCTAAACCGAGTTTCTCTTTTTCTTAAAATAAAATTTTTTTAAGAAAAAAAATCATCTTCGCAATGTTCTTTTTTTGGGGGGGAAAAAAGTCTCCCTTAACTAATATCAAATGTTCAAGATACTTGTACTTTCTTTTCAAGTCGAAATTCGATTCTTACTCCCACAGAAAAGAGCCAAATTAAGTGAACTTCAAAAATACATTTTTGTTATTCACATTTAACAATTCACTTATTTACTACCACTTTAAATTTAAATAAATTTTTTTCAATATTTTAAGAAAGTCATAGTCGAACATTGAAACTTTATTTTAAAAAAAAAAATCATACTGGAAAGTCAAGATTTGAAACAATTGAATACCACATGAGGAAAAGCACATTTGGAAAAAAATACATGACAAGTTTTGGGGAAAAAAATGTATATATTGACTCCTATAAAAATTTCAAAACGCTGACAATATCCTAAAGCCAACAAACAAGTTTTTATATCAAATAAAATATGGTAGATTATGTATTTTAGATTGAAAGATAATCAGTATATCTCAAATATATTAATTTCTGTATATAAATTTGTTTGGTATCTTTGATGAGCAGGATATTCGCCCAAAACCGAGCAAAGTTCCAAGTGGACCTCGACTACTTAGAAGACCAGACGTCCTGAAATGCAGCTATGATGGGAATTTGCCCATCACCAAGAACCCCAGCGCCTCAACTTAAACATGGTGGATCCTTTCACTTTCTTCTTTCTCCATTCTCTTTTTCTTCCTCTGTTTCGGCCTTTTTCGATATATTCTGTTTGAAGCTAAGGTCTGAATTTCCAAGGAGGGGAAAAACGATTACTGTTATAACAAAATAATTATCGTTCGGCTGTCTGTAATAGTACTGAAGTAAAAGATATACAAACTCTAAACTTTTATATTCGGTCCTTCGCTACTAGACTTCCTAAAATTGATGTCAACTATCATAGATCTCGAGAAATTACCCCGAGCTTCTTCGACCAAAATTCTGCCATTCTCTAACTGGCAAGTGCACAACAGAAGGCTGTCGGATCAAATGTTGGATTGGAATCAGTAGTAAATTGAAAGAGTACGATGGTGTGGCAGAGAAGGGCATTGGATTCCAAAGGACCAAAGCCAAAGATCCAACTCCACGCTTAGAACCTGCAAGAACTCAAGATTTTGAGAAATGGGATTTGTATCTATCATCAGGTTTTTGTCTGCTGTGTTCTTTTGAGAGAAAAATCAGATGCTAGGATGTAATATACATATATGGATGGACATTTTTTTCTGGACAGTTTAATTTTGGAATACCTGTTTCCCAGATTTCAGACT

mRNA sequence

TAATTCCACTGTTTGAGTGTCATCAGATATCTTCAGAGACTCTGTGAAACTTCCAAAAGAAGAAGGATATGGGTGATTATGGTCCACGGTTAGTGGTGCCTATAGATGTGAAGAAGAAGCCATGCCAACAGAAACTTCCACTTCACAACCGGTGGCACCCGGAGATACCGCCGGTGGCGGAGGTCAAAGTGGATGAGTTCTTCAGAGTTGAAATGGTGGATTTCAGCGGCAGAGGAATCACCAATGACTACTCTGCTGAGGACATTAAACATGCTGATACCTCTGTTACACATTATCTGAGTGGTCCAATTAGAGTTGTGGACAAGGATGGGGTTCCAGCCAAGCCAGGGGATTTACTCGTAGTCGAAATCTGCAACTTGGGTCCTCTCCCAGGAGATGAATGGGGTTATACTGCAACATTTGACAGAGAAAATGGAGGTGGTTTTCTTACTGACCATTTTCCTTGTGCAACCAAAGCTATTTGGTATTTTGAAGGAATATATGCATACTCTCCTCAAATCCCAGGAGTTCGATTCCCAGGTTTAACTCACCCTGGAGTGATTGGGACTGCACCATCGGCAGAACTTCTGAATATTTGGAATGAAAGGGAAAGGCACGTTCAAGAAAATGGCCTTCAAACTCTAAAACTTAGTGAGGTTTTGCATTCACGGCCATTGGCAAACCTACCAACAGCTAAAGGTTGCGCTCTTGGAAAGATCAAAGAGGGCACAGAAGAATGGGAAAGAATTGCCAATGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGAAACTGTGACATCAAGAACCTCAGTAGAGGTTCAAAAGTATACCTTCCAGTATTCGTGGAAGGGGCAAACTTGAGCACAGGTGACATGCACTTCTCCCAAGGAGATGGTGAAGTCTCATTTTGTGGAGCAATTGAAATGAGTGGTTTTCTAGAGCTCAAGTGCGATATTATAAGGGGCGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTAAACCCAATCTTCGAGATAGGTCCAGTTGAACCAAGATTCTCAGAATGGCTGGTTTTTGAAGGCATCAGCGTGGATGAAAGTGGGAGGCAGCATTTTCTTGACGCGAGTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATCGACTATCTCCACAAATTTGGGTACTCAAAGGAACAGGCTTATCTGCTGCTGTCGTGCTGCCCATGCGAAGGAAGGATCTCTGGGATTGTTGATTCACCAAATGCTGTGGCTACCTTGGCGATCCCCACCGCCATTTTCGACCAGGATATTCGCCCAAAACCGAGCAAAGTTCCAAGTGGACCTCGACTACTTAGAAGACCAGACGTCCTGAAATGCAGCTATGATGGGAATTTGCCCATCACCAAGAACCCCAGCGCCTCAACTTAAACATGGTGGATCCTTTCACTTTCTTCTTTCTCCATTCTCTTTTTCTTCCTCTGTTTCGGCCTTTTTCGATATATTCTGTTTGAAGCTAAGGTCTGAATTTCCAAGGAGGGGAAAAACGATTACTGTTATAACAAAATAATTATCGTTCGGCTGTCTGTAATAGTACTGAAGTAAAAGATATACAAACTCTAAACTTTTATATTCGGTCCTTCGCTACTAGACTTCCTAAAATTGATGTCAACTATCATAGATCTCGAGAAATTACCCCGAGCTTCTTCGACCAAAATTCTGCCATTCTCTAACTGGCAAGTGCACAACAGAAGGCTGTCGGATCAAATGTTGGATTGGAATCAGTAGTAAATTGAAAGAGTACGATGGTGTGGCAGAGAAGGGCATTGGATTCCAAAGGACCAAAGCCAAAGATCCAACTCCACGCTTAGAACCTGCAAGAACTCAAGATTTTGAGAAATGGGATTTGTATCTATCATCAGGTTTTTGTCTGCTGTGTTCTTTTGAGAGAAAAATCAGATGCTAGGATGTAATATACATATATGGATGGACATTTTTTTCTGGACAGTTTAATTTTGGAATACCTGTTTCCCAGATTTCAGACT

Coding sequence (CDS)

ATGGGTGATTATGGTCCACGGTTAGTGGTGCCTATAGATGTGAAGAAGAAGCCATGCCAACAGAAACTTCCACTTCACAACCGGTGGCACCCGGAGATACCGCCGGTGGCGGAGGTCAAAGTGGATGAGTTCTTCAGAGTTGAAATGGTGGATTTCAGCGGCAGAGGAATCACCAATGACTACTCTGCTGAGGACATTAAACATGCTGATACCTCTGTTACACATTATCTGAGTGGTCCAATTAGAGTTGTGGACAAGGATGGGGTTCCAGCCAAGCCAGGGGATTTACTCGTAGTCGAAATCTGCAACTTGGGTCCTCTCCCAGGAGATGAATGGGGTTATACTGCAACATTTGACAGAGAAAATGGAGGTGGTTTTCTTACTGACCATTTTCCTTGTGCAACCAAAGCTATTTGGTATTTTGAAGGAATATATGCATACTCTCCTCAAATCCCAGGAGTTCGATTCCCAGGTTTAACTCACCCTGGAGTGATTGGGACTGCACCATCGGCAGAACTTCTGAATATTTGGAATGAAAGGGAAAGGCACGTTCAAGAAAATGGCCTTCAAACTCTAAAACTTAGTGAGGTTTTGCATTCACGGCCATTGGCAAACCTACCAACAGCTAAAGGTTGCGCTCTTGGAAAGATCAAAGAGGGCACAGAAGAATGGGAAAGAATTGCCAATGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGAAACTGTGACATCAAGAACCTCAGTAGAGGTTCAAAAGTATACCTTCCAGTATTCGTGGAAGGGGCAAACTTGAGCACAGGTGACATGCACTTCTCCCAAGGAGATGGTGAAGTCTCATTTTGTGGAGCAATTGAAATGAGTGGTTTTCTAGAGCTCAAGTGCGATATTATAAGGGGCGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTAAACCCAATCTTCGAGATAGGTCCAGTTGAACCAAGATTCTCAGAATGGCTGGTTTTTGAAGGCATCAGCGTGGATGAAAGTGGGAGGCAGCATTTTCTTGACGCGAGTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATCGACTATCTCCACAAATTTGGGTACTCAAAGGAACAGGCTTATCTGCTGCTGTCGTGCTGCCCATGCGAAGGAAGGATCTCTGGGATTGTTGATTCACCAAATGCTGTGGCTACCTTGGCGATCCCCACCGCCATTTTCGACCAGGATATTCGCCCAAAACCGAGCAAAGTTCCAAGTGGACCTCGACTACTTAGAAGACCAGACGTCCTGAAATGCAGCTATGATGGGAATTTGCCCATCACCAAGAACCCCAGCGCCTCAACTTAA

Protein sequence

MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITNDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGRENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAST
Homology
BLAST of Lcy04g015300 vs. ExPASy Swiss-Prot
Match: Q50228 (Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 9.0e-118
Identity = 207/427 (48.48%), Postives = 290/427 (67.92%), Query Frame = 0

Query: 7   RLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITNDYSAEDI 66
           + +V +D+ KKP +Q   +HNRWHP++P +A VK  + FRVE +D++G  I N+ SA D+
Sbjct: 2   KTIVKLDLDKKPWEQDGQIHNRWHPDLPMIAMVKPGDEFRVECMDWTGGQIGNNDSANDV 61

Query: 67  KHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDRENGGGF 126
           +  D +  HYLSGPI V   +G  A+PGDL+VV+I ++G     +WG+   F +ENGGGF
Sbjct: 62  RDVDLTQVHYLSGPIGV---EG--AEPGDLMVVDILDVGTFDDSQWGFNGLFAKENGGGF 121

Query: 127 LTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNERERHVQE 186
           LTDHFP A+K IW F G+Y  S  +P VR+ G+ HPG+IG  PS ELL+ WN+RE     
Sbjct: 122 LTDHFPEASKTIWDFHGVYTTSRHVPKVRYAGIMHPGLIGCLPSKELLDTWNKRE----- 181

Query: 187 NGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGRENGGNCDI 246
                L  ++     PLA  PT++   +G++    +  ++ A E ART+P R++GGNCDI
Sbjct: 182 ---GDLIATDPDRVPPLACPPTSQSAVMGRL--SGDAAKKAAAEGARTVPPRDHGGNCDI 241

Query: 247 KNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIRGGMKEY 306
           KNL++GS+VY PV+V+   LS GD+HFSQGDGE++FCGAIEM+G+L++K  +I+ G+K+Y
Sbjct: 242 KNLTKGSRVYFPVYVKDGGLSMGDLHFSQGDGEITFCGAIEMAGYLDIKVGLIKDGVKKY 301

Query: 307 LTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAVLNAIDY 366
                      NP+F+  P+ P + ++++FEGISVDE+G+QH+LD  +AY++A LNAI+Y
Sbjct: 302 GIK--------NPVFQPSPITPTYRDYMIFEGISVDEAGKQHYLDVHIAYRQACLNAIEY 361

Query: 367 LHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSKVPSGPR 426
           L KFGYS EQA  +L   P EG ISGIVD PNA ATL IPT IF+ DIRP       GP+
Sbjct: 362 LKKFGYSGEQAVSILGTAPVEGHISGIVDIPNACATLWIPTEIFEFDIRPN----ADGPK 401

Query: 427 LLRRPDV 434
           ++  P V
Sbjct: 422 IMVPPGV 401

BLAST of Lcy04g015300 vs. ExPASy Swiss-Prot
Match: Q9URY7 (Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.04 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 9.6e-112
Identity = 207/415 (49.88%), Postives = 272/415 (65.54%), Query Frame = 0

Query: 6   PRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITNDYSAED 65
           PR +V +D+  KP  ++  LHNRWHP+IP    +   E  ++E +D++G  I ND SAED
Sbjct: 3   PRTIVKVDL-NKPAWEQPNLHNRWHPDIPFAESIDEGETVKIECLDWTGGQIKNDDSAED 62

Query: 66  IKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDRENGGG 125
           IK+ D +  HYLSGP  +       A+PGD+LVVEI ++ PL    WGY+  F +ENGGG
Sbjct: 63  IKNVDLTRIHYLSGPFEIKG-----AEPGDVLVVEIQDVQPLENQPWGYSGIFAKENGGG 122

Query: 126 FLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNERERHVQ 185
           FL +H+P A KA++ FEGI+  S  IPGVRFPGL HPG+IGTAPS E+L  WN RE  + 
Sbjct: 123 FLDEHYPKAAKAVFDFEGIFCSSRHIPGVRFPGLIHPGIIGTAPSKEILAEWNRREGAL- 182

Query: 186 ENGLQTLKLSEVLHS-RPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR-ENGGN 245
                   ++E  HS   +A LP A   A   I    +    +A+E ARTIPGR ENGGN
Sbjct: 183 --------VAENPHSTHVMAQLPNA-SYAFAGILADEKLSATVASEGARTIPGRPENGGN 242

Query: 246 CDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIRGGM 305
           CDIKNLSRGSKV+LPV V GA LS GD+HFSQGDGE+SFCGAIEM+G + +KC I++ G+
Sbjct: 243 CDIKNLSRGSKVFLPVHVPGAKLSIGDLHFSQGDGEISFCGAIEMAGSITIKCKILKNGI 302

Query: 306 KEYLTPMGPTPLHVNPIFEIGPVEPRF--SEWLVFEGISVDESGRQHFLDASVAYKRAVL 365
            +            +P++  GPVEP F  S +L FEG SVDESG+QH+L  + AY++  L
Sbjct: 303 SDLAMK--------SPMYLPGPVEPHFSPSRYLTFEGFSVDESGKQHYLCTTTAYRQTCL 362

Query: 366 NAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRP 417
             I+Y  +FGY+  Q YLLLSC P +G ++GIVD PN+  T+ +P  IF+ D+ P
Sbjct: 363 RVIEYFRRFGYNDYQLYLLLSCAPIQGHVAGIVDIPNSCTTIGVPMDIFEFDVSP 393

BLAST of Lcy04g015300 vs. ExPASy Swiss-Prot
Match: Q07838 (Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.5e-101
Identity = 196/391 (50.13%), Postives = 256/391 (65.47%), Query Frame = 0

Query: 6   PRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITNDYSAED 65
           P +V  +D  K    Q +P HNRWHP+IP  A VK    FR+E  +++   I N+ SA D
Sbjct: 2   PEVVFSVDHSKSMRDQAVPGHNRWHPDIPAAATVKPGSEFRIECKEWTDGQIGNNDSAND 61

Query: 66  IKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLP-------GDEWGYTATF 125
           ++  D +  H LSGPI+V   +G  A+PGDLL+V+I ++GP+P       G+ WGY+  F
Sbjct: 62  VRDVDLAPCHMLSGPIKV---EG--AEPGDLLIVDILDIGPVPQTNGPNCGEGWGYSGIF 121

Query: 126 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 185
            + NGGGFLTD++P A KAIW F G    S  +PGVR+ G+THPG+ GTAPS +LL  WN
Sbjct: 122 AKVNGGGFLTDYYPDAYKAIWDFHGQQCTSRHVPGVRYTGITHPGLFGTAPSPDLLAKWN 181

Query: 186 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 245
           ERER         L  ++     PLA  P   G  LG    G +  + IAN+ ART+P R
Sbjct: 182 ERER--------ALIATDPDRVPPLALPPLVDG-TLGGTASG-DLLQAIANDGARTVPPR 241

Query: 246 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 305
           ENGGN DIKN +RGS+++ PVFVEGA LS GD+HFSQGDGE++FCGAIEM GF+++  D+
Sbjct: 242 ENGGNHDIKNFTRGSRIFYPVFVEGAMLSGGDLHFSQGDGEINFCGAIEMGGFIDMHVDL 301

Query: 306 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDES-GRQHFLDASVAYK 365
           I+GGM+ Y    G T    NPIF  G VEP +SEWL F GISVD +  R  ++DA++AY+
Sbjct: 302 IKGGMETY----GVT---TNPIFMPGRVEPLYSEWLTFIGISVDHAENRNAYMDATMAYR 361

Query: 366 RAVLNAIDYLHKFGYSKEQAYLLLSCCPCEG 389
            A LNAI+YL K+GY+ EQAYL+L   P EG
Sbjct: 362 NACLNAIEYLKKWGYTGEQAYLILGTSPIEG 370

BLAST of Lcy04g015300 vs. ExPASy TrEMBL
Match: A0A5D3CLP5 (Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004150 PE=4 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.6e-261
Identity = 429/454 (94.49%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 1   MGD--YGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGIT 60
           MGD  YGPR+VVPID+KKKP QQK PLHNRWHPEIPP AEVKV EFFR+EMVDFSG GIT
Sbjct: 1   MGDSGYGPRIVVPIDLKKKPWQQKFPLHNRWHPEIPPAAEVKVAEFFRIEMVDFSGGGIT 60

Query: 61  NDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120
            DYSA+DIKH D SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF
Sbjct: 61  KDYSADDIKHVDNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120

Query: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 180
           DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPG+IGTAPSAELL IWN
Sbjct: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGIIGTAPSAELLKIWN 180

Query: 181 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 240
           ERERHVQENGLQTLKL EV+HSRPLA+LP+AKGCALGKIKEGTEEWERIANEAARTIPGR
Sbjct: 181 ERERHVQENGLQTLKLCEVVHSRPLAHLPSAKGCALGKIKEGTEEWERIANEAARTIPGR 240

Query: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300
           ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI
Sbjct: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300

Query: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKR 360
           IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKR
Sbjct: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKR 360

Query: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKP 420
           AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNA+ TLAIPTAIFDQDIRPKP
Sbjct: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIFDQDIRPKP 420

Query: 421 SKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           SKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+T
Sbjct: 421 SKVPIGPRLLRRPDVLKCSYDGNLPITKNPSATT 454

BLAST of Lcy04g015300 vs. ExPASy TrEMBL
Match: A0A1S3B102 (formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.6e-261
Identity = 429/454 (94.49%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 1   MGD--YGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGIT 60
           MGD  YGPR+VVPID+KKKP QQK PLHNRWHPEIPP AEVKV EFFR+EMVDFSG GIT
Sbjct: 1   MGDSGYGPRIVVPIDLKKKPWQQKFPLHNRWHPEIPPAAEVKVAEFFRIEMVDFSGGGIT 60

Query: 61  NDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120
            DYSA+DIKH D SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF
Sbjct: 61  KDYSADDIKHVDNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120

Query: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 180
           DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPG+IGTAPSAELL IWN
Sbjct: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGIIGTAPSAELLKIWN 180

Query: 181 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 240
           ERERHVQENGLQTLKL EV+HSRPLA+LP+AKGCALGKIKEGTEEWERIANEAARTIPGR
Sbjct: 181 ERERHVQENGLQTLKLCEVVHSRPLAHLPSAKGCALGKIKEGTEEWERIANEAARTIPGR 240

Query: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300
           ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI
Sbjct: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300

Query: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKR 360
           IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKR
Sbjct: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKR 360

Query: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKP 420
           AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNA+ TLAIPTAIFDQDIRPKP
Sbjct: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIFDQDIRPKP 420

Query: 421 SKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           SKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+T
Sbjct: 421 SKVPIGPRLLRRPDVLKCSYDGNLPITKNPSATT 454

BLAST of Lcy04g015300 vs. ExPASy TrEMBL
Match: A0A0A0KGH0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 430/453 (94.92%), Postives = 440/453 (97.13%), Query Frame = 0

Query: 1   MGD--YGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGIT 60
           MGD  YGPRLVVPID+KKKP QQ+ PLHNRWHPEIPPVAEVKV EFFRVEMVDFSG GIT
Sbjct: 1   MGDSGYGPRLVVPIDLKKKPWQQEFPLHNRWHPEIPPVAEVKVAEFFRVEMVDFSGGGIT 60

Query: 61  NDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120
            DYSA+DIKH D SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF
Sbjct: 61  QDYSADDIKHVDNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120

Query: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 180
           DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELL IWN
Sbjct: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLKIWN 180

Query: 181 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 240
           ERER VQENGLQTLKL EV+HSRPLANLP+AKGCALGKIKEGTEEWERIANEAARTIPGR
Sbjct: 181 ERERDVQENGLQTLKLCEVVHSRPLANLPSAKGCALGKIKEGTEEWERIANEAARTIPGR 240

Query: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300
           ENGGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDI
Sbjct: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300

Query: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKR 360
           IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKR
Sbjct: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKR 360

Query: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKP 420
           AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ TLAIPTAIFDQDIRPKP
Sbjct: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFDQDIRPKP 420

Query: 421 SKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAS 452
           SKVP+GPRLLRRPDVLKCSYDGNLPITKNPSAS
Sbjct: 421 SKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 453

BLAST of Lcy04g015300 vs. ExPASy TrEMBL
Match: A0A6J1GEJ7 (uncharacterized protein LOC111453252 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453252 PE=4 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 1.9e-259
Identity = 426/452 (94.25%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           MGDYGPRLVV +DVKK P QQKLPLHNRWHPEIPPVAEVKVDE F+VEMVDFSG  IT D
Sbjct: 1   MGDYGPRLVVSVDVKKNPWQQKLPLHNRWHPEIPPVAEVKVDELFKVEMVDFSGGAITKD 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
           YSAED+KHAD S THYLSGPIRVVDKDGVPA PGDLLVVEICNLGPLPGDEWGYTATFDR
Sbjct: 61  YSAEDVKHADNSCTHYLSGPIRVVDKDGVPAMPGDLLVVEICNLGPLPGDEWGYTATFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           E HVQENGLQTLKL EVLHSRPLANLP+AKGC LGKIKEGTEEWERIANEAARTIPGREN
Sbjct: 181 ESHVQENGLQTLKLCEVLHSRPLANLPSAKGCVLGKIKEGTEEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR
Sbjct: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
           GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV
Sbjct: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ATLAIPTAIFDQDIRPK SK
Sbjct: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDAPNAMATLAIPTAIFDQDIRPKASK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR+L+RPDVLKCSYDGNLPITKNPSA++
Sbjct: 421 VPVGPRVLKRPDVLKCSYDGNLPITKNPSATS 452

BLAST of Lcy04g015300 vs. ExPASy TrEMBL
Match: A0A6J1IIP2 (uncharacterized protein LOC111477820 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477820 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 426/452 (94.25%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           MGDYGPRLVV +DVKK P QQKLPLHNRWHPEIPPVAEVKVDE F+VEMVDFSG  IT D
Sbjct: 1   MGDYGPRLVVSVDVKKNPWQQKLPLHNRWHPEIPPVAEVKVDELFKVEMVDFSGGAITKD 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
           YSAED+KHAD S THYLSGPIRVVDKDGVPA PGDLLVVEICNLGPLPGDEWGYTATFDR
Sbjct: 61  YSAEDVKHADNSSTHYLSGPIRVVDKDGVPAMPGDLLVVEICNLGPLPGDEWGYTATFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           E HVQENGLQTLKL EVLHSRPLANLP+AKGC LGKIKEGTEEWERIANEAARTIPGREN
Sbjct: 181 ESHVQENGLQTLKLCEVLHSRPLANLPSAKGCVLGKIKEGTEEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR
Sbjct: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
           GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV
Sbjct: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ATLAIPTAIFDQDIRPK SK
Sbjct: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDAPNAMATLAIPTAIFDQDIRPKASK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR+L+RPDVLKCSYDGNLPITKNPSA++
Sbjct: 421 VPVGPRVLKRPDVLKCSYDGNLPITKNPSATS 452

BLAST of Lcy04g015300 vs. NCBI nr
Match: XP_038883824.1 (formamidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 912.1 bits (2356), Expect = 1.8e-261
Identity = 432/452 (95.58%), Postives = 440/452 (97.35%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           MGDYGPRLV+PIDVKKKP QQK  LHNRWHPEIPPVAEVKV E FRVEMVDFSG GIT D
Sbjct: 1   MGDYGPRLVMPIDVKKKPWQQKFSLHNRWHPEIPPVAEVKVAELFRVEMVDFSGGGITKD 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
           YSAEDIKHAD SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR
Sbjct: 61  YSAEDIKHADNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYS QIPGVRFPGLTHPGVIGTAPSAELL IWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSAQIPGVRFPGLTHPGVIGTAPSAELLKIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           ERHVQENGLQTLKL EV+HSRPLANLPTAKGC+LGKIKEGTEEWERIANEAARTIPGREN
Sbjct: 181 ERHVQENGLQTLKLCEVVHSRPLANLPTAKGCSLGKIKEGTEEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR
Sbjct: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
           GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKRAV
Sbjct: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNA+ATLAIPTAIFDQDIRP+PSK
Sbjct: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMATLAIPTAIFDQDIRPQPSK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPRLLRRPDVLKCSYDGNLPITKNPSA+T
Sbjct: 421 VPIGPRLLRRPDVLKCSYDGNLPITKNPSATT 452

BLAST of Lcy04g015300 vs. NCBI nr
Match: XP_008440367.1 (PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-260
Identity = 429/454 (94.49%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 1   MGD--YGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGIT 60
           MGD  YGPR+VVPID+KKKP QQK PLHNRWHPEIPP AEVKV EFFR+EMVDFSG GIT
Sbjct: 1   MGDSGYGPRIVVPIDLKKKPWQQKFPLHNRWHPEIPPAAEVKVAEFFRIEMVDFSGGGIT 60

Query: 61  NDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120
            DYSA+DIKH D SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF
Sbjct: 61  KDYSADDIKHVDNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120

Query: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 180
           DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPG+IGTAPSAELL IWN
Sbjct: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGIIGTAPSAELLKIWN 180

Query: 181 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 240
           ERERHVQENGLQTLKL EV+HSRPLA+LP+AKGCALGKIKEGTEEWERIANEAARTIPGR
Sbjct: 181 ERERHVQENGLQTLKLCEVVHSRPLAHLPSAKGCALGKIKEGTEEWERIANEAARTIPGR 240

Query: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300
           ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI
Sbjct: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300

Query: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKR 360
           IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKR
Sbjct: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKR 360

Query: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKP 420
           AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNA+ TLAIPTAIFDQDIRPKP
Sbjct: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIFDQDIRPKP 420

Query: 421 SKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           SKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+T
Sbjct: 421 SKVPIGPRLLRRPDVLKCSYDGNLPITKNPSATT 454

BLAST of Lcy04g015300 vs. NCBI nr
Match: XP_011657890.1 (uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hypothetical protein Csa_003261 [Cucumis sativus])

HSP 1 Score: 906.7 bits (2342), Expect = 7.7e-260
Identity = 430/453 (94.92%), Postives = 440/453 (97.13%), Query Frame = 0

Query: 1   MGD--YGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGIT 60
           MGD  YGPRLVVPID+KKKP QQ+ PLHNRWHPEIPPVAEVKV EFFRVEMVDFSG GIT
Sbjct: 1   MGDSGYGPRLVVPIDLKKKPWQQEFPLHNRWHPEIPPVAEVKVAEFFRVEMVDFSGGGIT 60

Query: 61  NDYSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120
            DYSA+DIKH D SVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF
Sbjct: 61  QDYSADDIKHVDNSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATF 120

Query: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWN 180
           DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELL IWN
Sbjct: 121 DRENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLKIWN 180

Query: 181 ERERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGR 240
           ERER VQENGLQTLKL EV+HSRPLANLP+AKGCALGKIKEGTEEWERIANEAARTIPGR
Sbjct: 181 ERERDVQENGLQTLKLCEVVHSRPLANLPSAKGCALGKIKEGTEEWERIANEAARTIPGR 240

Query: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300
           ENGGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDI
Sbjct: 241 ENGGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDI 300

Query: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKR 360
           IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDA+VAYKR
Sbjct: 301 IRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKR 360

Query: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKP 420
           AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ TLAIPTAIFDQDIRPKP
Sbjct: 361 AVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFDQDIRPKP 420

Query: 421 SKVPSGPRLLRRPDVLKCSYDGNLPITKNPSAS 452
           SKVP+GPRLLRRPDVLKCSYDGNLPITKNPSAS
Sbjct: 421 SKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 453

BLAST of Lcy04g015300 vs. NCBI nr
Match: XP_022950050.1 (uncharacterized protein LOC111453252 isoform X2 [Cucurbita moschata])

HSP 1 Score: 904.4 bits (2336), Expect = 3.8e-259
Identity = 426/452 (94.25%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           MGDYGPRLVV +DVKK P QQKLPLHNRWHPEIPPVAEVKVDE F+VEMVDFSG  IT D
Sbjct: 1   MGDYGPRLVVSVDVKKNPWQQKLPLHNRWHPEIPPVAEVKVDELFKVEMVDFSGGAITKD 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
           YSAED+KHAD S THYLSGPIRVVDKDGVPA PGDLLVVEICNLGPLPGDEWGYTATFDR
Sbjct: 61  YSAEDVKHADNSCTHYLSGPIRVVDKDGVPAMPGDLLVVEICNLGPLPGDEWGYTATFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           E HVQENGLQTLKL EVLHSRPLANLP+AKGC LGKIKEGTEEWERIANEAARTIPGREN
Sbjct: 181 ESHVQENGLQTLKLCEVLHSRPLANLPSAKGCVLGKIKEGTEEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR
Sbjct: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
           GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV
Sbjct: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ATLAIPTAIFDQDIRPK SK
Sbjct: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDAPNAMATLAIPTAIFDQDIRPKASK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR+L+RPDVLKCSYDGNLPITKNPSA++
Sbjct: 421 VPVGPRVLKRPDVLKCSYDGNLPITKNPSATS 452

BLAST of Lcy04g015300 vs. NCBI nr
Match: XP_022977497.1 (uncharacterized protein LOC111477820 isoform X2 [Cucurbita maxima])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 426/452 (94.25%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           MGDYGPRLVV +DVKK P QQKLPLHNRWHPEIPPVAEVKVDE F+VEMVDFSG  IT D
Sbjct: 1   MGDYGPRLVVSVDVKKNPWQQKLPLHNRWHPEIPPVAEVKVDELFKVEMVDFSGGAITKD 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
           YSAED+KHAD S THYLSGPIRVVDKDGVPA PGDLLVVEICNLGPLPGDEWGYTATFDR
Sbjct: 61  YSAEDVKHADNSSTHYLSGPIRVVDKDGVPAMPGDLLVVEICNLGPLPGDEWGYTATFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           E HVQENGLQTLKL EVLHSRPLANLP+AKGC LGKIKEGTEEWERIANEAARTIPGREN
Sbjct: 181 ESHVQENGLQTLKLCEVLHSRPLANLPSAKGCVLGKIKEGTEEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSKVYLPVFVEGANLS+GDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR
Sbjct: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSSGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
           GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV
Sbjct: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNA+ATLAIPTAIFDQDIRPK SK
Sbjct: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDAPNAMATLAIPTAIFDQDIRPKASK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR+L+RPDVLKCSYDGNLPITKNPSA++
Sbjct: 421 VPVGPRVLKRPDVLKCSYDGNLPITKNPSATS 452

BLAST of Lcy04g015300 vs. TAIR 10
Match: AT4G37550.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 796.6 bits (2056), Expect = 1.1e-230
Identity = 372/452 (82.30%), Postives = 408/452 (90.27%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           M    PR+VV +D+KKKP QQ  PLHNRWHPEIPPVAEVK  EFFRVEM+D  G  I ++
Sbjct: 1   MAPLSPRVVVKVDLKKKPWQQDQPLHNRWHPEIPPVAEVKAGEFFRVEMIDAMGGVIKDN 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
            SA DIK+   + TH+LSGPIRVVD++GV AK GDLL VEICNLGPLPGDEWG+T +FDR
Sbjct: 61  DSASDIKNLVLTTTHHLSGPIRVVDEEGVAAKAGDLLAVEICNLGPLPGDEWGFTGSFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPS ELL IWN+R
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSNELLRIWNDR 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           ER ++E+G+++L L EV+H RPLA LPT KGC LG I+EGT EWERIANEAARTIPGREN
Sbjct: 181 ERQLEESGVESLTLCEVVHQRPLACLPTTKGCLLGNIEEGTPEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSK+YLPVFVEGANLSTGDMHFSQGDGE+SFCGAIEMSGFLELKC+IIR
Sbjct: 241 GGNCDIKNLSRGSKIYLPVFVEGANLSTGDMHFSQGDGEISFCGAIEMSGFLELKCEIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
            GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+LDA+VAYKRAV
Sbjct: 301 NGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYLDATVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVDSPNAVATLAIPTAIFDQDIRPK  K
Sbjct: 361 LNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIFDQDIRPKNRK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR++R+PDVLK +YDG LPITKNPS+S+
Sbjct: 421 VPVGPRVVRKPDVLKSTYDGKLPITKNPSSSS 452

BLAST of Lcy04g015300 vs. TAIR 10
Match: AT4G37560.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 788.9 bits (2036), Expect = 2.2e-228
Identity = 367/452 (81.19%), Postives = 403/452 (89.16%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           M    PR+VV +D+KK+P QQ  PLHNRWHPEIP VAEVK  E FRVEMVD++G  +  D
Sbjct: 1   MAPASPRVVVKVDLKKRPWQQTQPLHNRWHPEIPSVAEVKTGELFRVEMVDWTGGAVKED 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
            SA DIK  D S  HYLSGPI+VVD+DGV A+PGDLL VEICNLGPLPGDEWG+TA+FDR
Sbjct: 61  GSAGDIKSIDLSTVHYLSGPIKVVDEDGVAARPGDLLAVEICNLGPLPGDEWGFTASFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPG+IGTAPS ELL IWNER
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGIIGTAPSKELLRIWNER 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           ER ++E G+++L L EV+H RPLA+LPT KGC LG I+E T EWERIA EAARTIPGREN
Sbjct: 181 ERQLEEKGVESLTLCEVVHQRPLASLPTTKGCLLGNIEERTSEWERIAKEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLS GSK+YLPVFVEGANLSTGDMHFSQGDGE+SFCGAIEMSGFLELKC+IIR
Sbjct: 241 GGNCDIKNLSSGSKIYLPVFVEGANLSTGDMHFSQGDGEISFCGAIEMSGFLELKCEIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
            GM+EYLTPMGPT LHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQH+LDA+VAYKRAV
Sbjct: 301 NGMQEYLTPMGPTTLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHYLDATVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYL KFGYSKEQ YLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPK  K
Sbjct: 361 LNAIDYLFKFGYSKEQVYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKTRK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP+G R++++PDV+K +YDG LPITKN S+S+
Sbjct: 421 VPTGARIVKKPDVMKSTYDGKLPITKNSSSSS 452

BLAST of Lcy04g015300 vs. TAIR 10
Match: AT4G37550.3 (Acetamidase/Formamidase family protein )

HSP 1 Score: 755.4 bits (1949), Expect = 2.7e-218
Identity = 358/452 (79.20%), Postives = 393/452 (86.95%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           M    PR+VV +D+KKKP QQ  PLHNRWHPEIPPVAEVK  EFFRVEM+D  G  I ++
Sbjct: 1   MAPLSPRVVVKVDLKKKPWQQDQPLHNRWHPEIPPVAEVKAGEFFRVEMIDAMGGVIKDN 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
            SA DIK+   + TH+LSGPIRVVD++GV AK GDLL VEICNLGPLPGDEWG+T +FDR
Sbjct: 61  DSASDIKNLVLTTTHHLSGPIRVVDEEGVAAKAGDLLAVEICNLGPLPGDEWGFTGSFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPS ELL IWN+R
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSNELLRIWNDR 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           ER ++E+G+++L L E                    I+EGT EWERIANEAARTIPGREN
Sbjct: 181 ERQLEESGVESLTLCE--------------------IEEGTPEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSK+YLPVFVEGANLSTGDMHFSQGDGE+SFCGAIEMSGFLELKC+IIR
Sbjct: 241 GGNCDIKNLSRGSKIYLPVFVEGANLSTGDMHFSQGDGEISFCGAIEMSGFLELKCEIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
            GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+LDA+VAYKRAV
Sbjct: 301 NGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYLDATVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIFDQDIRPKPSK 420
           LNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVDSPNAVATLAIPTAIFDQDIRPK  K
Sbjct: 361 LNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIFDQDIRPKNRK 420

Query: 421 VPSGPRLLRRPDVLKCSYDGNLPITKNPSAST 453
           VP GPR++R+PDVLK +YDG LPITKNPS+S+
Sbjct: 421 VPVGPRVVRKPDVLKSTYDGKLPITKNPSSSS 432

BLAST of Lcy04g015300 vs. TAIR 10
Match: AT4G37550.2 (Acetamidase/Formamidase family protein )

HSP 1 Score: 665.2 bits (1715), Expect = 3.7e-191
Identity = 309/376 (82.18%), Postives = 338/376 (89.89%), Query Frame = 0

Query: 1   MGDYGPRLVVPIDVKKKPCQQKLPLHNRWHPEIPPVAEVKVDEFFRVEMVDFSGRGITND 60
           M    PR+VV +D+KKKP QQ  PLHNRWHPEIPPVAEVK  EFFRVEM+D  G  I ++
Sbjct: 1   MAPLSPRVVVKVDLKKKPWQQDQPLHNRWHPEIPPVAEVKAGEFFRVEMIDAMGGVIKDN 60

Query: 61  YSAEDIKHADTSVTHYLSGPIRVVDKDGVPAKPGDLLVVEICNLGPLPGDEWGYTATFDR 120
            SA DIK+   + TH+LSGPIRVVD++GV AK GDLL VEICNLGPLPGDEWG+T +FDR
Sbjct: 61  DSASDIKNLVLTTTHHLSGPIRVVDEEGVAAKAGDLLAVEICNLGPLPGDEWGFTGSFDR 120

Query: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSAELLNIWNER 180
           ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPS ELL IWN+R
Sbjct: 121 ENGGGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFPGLTHPGVIGTAPSNELLRIWNDR 180

Query: 181 ERHVQENGLQTLKLSEVLHSRPLANLPTAKGCALGKIKEGTEEWERIANEAARTIPGREN 240
           ER ++E+G+++L L EV+H RPLA LPT KGC LG I+EGT EWERIANEAARTIPGREN
Sbjct: 181 ERQLEESGVESLTLCEVVHQRPLACLPTTKGCLLGNIEEGTPEWERIANEAARTIPGREN 240

Query: 241 GGNCDIKNLSRGSKVYLPVFVEGANLSTGDMHFSQGDGEVSFCGAIEMSGFLELKCDIIR 300
           GGNCDIKNLSRGSK+YLPVFVEGANLSTGDMHFSQGDGE+SFCGAIEMSGFLELKC+IIR
Sbjct: 241 GGNCDIKNLSRGSKIYLPVFVEGANLSTGDMHFSQGDGEISFCGAIEMSGFLELKCEIIR 300

Query: 301 GGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDASVAYKRAV 360
            GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+LDA+VAYKRAV
Sbjct: 301 NGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYLDATVAYKRAV 360

Query: 361 LNAIDYLHKFGYSKEQ 377
           LNAIDYL KFGYSKEQ
Sbjct: 361 LNAIDYLFKFGYSKEQ 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q502289.0e-11848.48Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1[more]
Q9URY79.6e-11249.88Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Q078381.5e-10150.13Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CLP57.6e-26194.49Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B1027.6e-26194.49formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1[more]
A0A0A0KGH03.8e-26094.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1[more]
A0A6J1GEJ71.9e-25994.25uncharacterized protein LOC111453252 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IIP22.4e-25994.25uncharacterized protein LOC111477820 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038883824.11.8e-26195.58formamidase-like isoform X2 [Benincasa hispida][more]
XP_008440367.11.6e-26094.49PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-li... [more]
XP_011657890.17.7e-26094.92uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hy... [more]
XP_022950050.13.8e-25994.25uncharacterized protein LOC111453252 isoform X2 [Cucurbita moschata][more]
XP_022977497.15.0e-25994.25uncharacterized protein LOC111477820 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G37550.11.1e-23082.30Acetamidase/Formamidase family protein [more]
AT4G37560.12.2e-22881.19Acetamidase/Formamidase family protein [more]
AT4G37550.32.7e-21879.20Acetamidase/Formamidase family protein [more]
AT4G37550.23.7e-19182.18Acetamidase/Formamidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.580coord: 150..301
e-value: 1.7E-21
score: 78.3
NoneNo IPR availableGENE3D2.60.120.580coord: 12..141
e-value: 1.3E-17
score: 65.6
NoneNo IPR availableGENE3D3.10.28.20coord: 345..412
e-value: 2.3E-5
score: 26.2
NoneNo IPR availablePANTHERPTHR31891:SF8FORMAMIDASE-RELATEDcoord: 1..450
NoneNo IPR availableSUPERFAMILY141130Acetamidase/Formamidase-likecoord: 24..411
IPR004304Acetamidase/FormamidasePFAMPF03069FmdA_AmdAcoord: 12..412
e-value: 1.1E-160
score: 534.6
IPR004304Acetamidase/FormamidasePANTHERPTHR31891FORMAMIDASE C869.04-RELATEDcoord: 1..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g015300.1Lcy04g015300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides