Homology
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 547/759 (72.07%), Postives = 636/759 (83.79%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
++ + LLL F S ++ D+ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE+
Sbjct: 6 LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65
Query: 61 IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYP 120
+Y Y N +HGFSTRLT EEA L +QPG+++V+PE RYELHTTRTP FLGLD++ ADL+P
Sbjct: 66 LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125
Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
E+ S S+V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGAR+
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185
Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
F++GYE+T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RA
Sbjct: 186 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 245
Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
RVA YKVCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+G
Sbjct: 246 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 305
Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
IL+SCSAGNAGPS SLSN +PWITTVGAGTLDRDFPA LG+GKNF+GVSL++G+ LP
Sbjct: 306 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 365
Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
LLPFIYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM
Sbjct: 366 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 425
Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
+LANTAANGEELVAD+HLLPAT V + GDIIR Y+ ++P PT +I GT +G++PSPV
Sbjct: 426 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 485
Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
VAAFSSRGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSC
Sbjct: 486 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 545
Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
PHVSGLAAL+K HP+WSPAAIRSALMTTAY YK+G+ + D ATGKPSTPFDHGAGHV
Sbjct: 546 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVS 605
Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
P +A NPGL+YDLT +DYL FLCALNYTS QI S++RR++TCD K YSV DLNYPSFAV
Sbjct: 606 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665
Query: 661 VFDGVLGGGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKSYT 720
DGV K+TRT+T+VG GTY V ++SET VKISVEP L+F EANEKKSYT
Sbjct: 666 NVDGV-----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 725
Query: 721 VTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
VTFT S+ P + FG IEWSDGKHVVGSP+A SWT
Sbjct: 726 VTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 811.6 bits (2095), Expect = 7.5e-234
Identity = 415/737 (56.31%), Postives = 533/737 (72.32%), Query Frame = 0
Query: 24 KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNNVVHGFSTRLTAEEAQR- 83
KKTYI+ + PESF H WY S L S S ++Y Y HGFS L + EA
Sbjct: 27 KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86
Query: 84 LESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVSEVIIGVLDTGVWPESKS 143
L S IL + + Y LHTTRTP+FLGL+ ++ +S + VIIGVLDTGVWPES+S
Sbjct: 87 LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146
Query: 144 FDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE-ATLGPIDESKESRSPR 203
FDDT + +PS WKGECESG++F + CN+KLIGAR FSKG++ A+ G +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206
Query: 204 DDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFSSDILAAI 263
D DGHGTHT+TTAAGS V NAS GYA+GTARGMA RARVA YKVCW+ GCF SDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266
Query: 264 EKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPSPYSLSNTSP 323
++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SCSAGN+GP+ S++N +P
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326
Query: 324 WITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGTLLPFIYAANASNSANGNLCM 383
W+ TVGAGTLDRDFPA+ +LG+GK +GVSLY G + L +Y N NS++ NLC+
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386
Query: 384 TGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPAT 443
G+L V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANTAA+GEELVADSHLLPA
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446
Query: 444 AVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDII 503
AV + GD++R+Y+ S+ KPT ++F+GT L ++PSPVVAAFSSRGPN++TP++LKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506
Query: 504 APGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAI 563
PGVNILAGWS ++GP+GL D RR FNI+SGTSMSCPH+SGLA L+K AHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566
Query: 564 RSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFL 623
+SALMTTAY + D A S P+ HG+GHVDP AL+PGLVYD++ ++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626
Query: 624 CALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVVFDGVLGGGSSVVKHTRTLTNV 683
C+L+YT I ++ +R S K LNYPSF+V+F GG VV++TR +TNV
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-----GGKRVVRYTREVTNV 686
Query: 684 GSPGT-YKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATGSAAPPSAEGFGRI 743
G+ + YKV+++ V ISV+P LSF EKK YTVTF + G + AE FG I
Sbjct: 687 GAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE-FGSI 746
Query: 744 EWSDGKHVVGSPIAFSW 758
WS+ +H V SP+AFSW
Sbjct: 747 TWSNPQHEVRSPVAFSW 751
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match:
Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)
HSP 1 Score: 780.8 bits (2015), Expect = 1.4e-224
Identity = 397/750 (52.93%), Postives = 521/750 (69.47%), Query Frame = 0
Query: 24 KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 83
KKTY++HM K MP + +HL WY S + SV+ + ++Y Y HG + +
Sbjct: 34 KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93
Query: 84 LTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESTSVSEVIIGVL 143
LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++ ++ E + +V++GVL
Sbjct: 94 LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153
Query: 144 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYEATLGPID 203
DTG+WPES+SF+DTG+ PVP++W+G CE+G F NCNRK++GAR F +GYEA G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213
Query: 204 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 263
E E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273
Query: 264 FSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPS 323
FSSDIL+A+++AV D V VLS+SLGGG+S Y +DS++ F AME G+ +SCSAGN GP
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333
Query: 324 PYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 383
P SL+N SPWITTVGA T+DRDFPA V +G + F GVSLY+G+ + P +Y
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393
Query: 384 NASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 443
NAS+ + C+ G L VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453
Query: 444 ELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPN 503
ELVADSH+LPA AV + G +I++Y ++ K T ++ GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513
Query: 504 SITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALI 563
++ ++LKPD++APGVNILA W+ + PS L+ D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573
Query: 564 KGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLV 623
K HPDWSPAAI+SALMTTAY + + D + PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633
Query: 624 YDLTVDDYLNFLCALNYTSSQINSLARR-DFTCDSGKKYSVNDLNYPSFAVVFDGVLGGG 683
YD+ +Y FLC + + SQ+ + + TC + +LNYP+ + +F
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693
Query: 684 SSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATG 743
+ ++ RT+TNVG +YKVS+ S K ++V+P++L+FT ++K SYTVTF T
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 753
Query: 744 SAAPPSAEGFGRIEWSDGKHVVGSPIAFSW 758
P FG + W H V SP+ +W
Sbjct: 754 MKRPE----FGGLVWKSTTHKVRSPVIITW 776
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match:
Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)
HSP 1 Score: 772.3 bits (1993), Expect = 5.0e-222
Identity = 408/764 (53.40%), Postives = 517/764 (67.67%), Query Frame = 0
Query: 9 LLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 68
LLLCFFS S ++ ++YIVH+ + P F H +W+ S L+S+ + A ++Y+Y+
Sbjct: 15 LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74
Query: 69 NVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVS 128
VHGFS RL+ + L P +++V+P+ E+HTT TP FLG +N+ L+ S
Sbjct: 75 RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134
Query: 129 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE 188
+VI+GVLDTG+WPE SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194
Query: 189 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 248
+ +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254
Query: 249 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVASGAFAAMEKGIL 308
YK+CW GGC+ SDILAA+++AV D V+V+S+S+G G +Y+ DS+A GAF A GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314
Query: 309 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGT 368
+SCSAGN+GP+P + +N +PWI TVGA T+DR+F A GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374
Query: 369 LLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 428
L +Y S LC G L V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434
Query: 429 ANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLG-IQPSPVV 488
ANTA +GEEL ADSHL+PAT V GD IR Y+ + PT I F GT +G PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494
Query: 489 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 548
AAFSSRGPN +TP +LKPD+IAPGVNILAGW+ VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554
Query: 549 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 608
HVSGLAAL++ AHPDWSPAAI+SAL+TTAY +G+ I+D ATGK S F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614
Query: 609 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDSGKKYSVNDLNYPSF 668
ALNPGLVYD+ V +Y+ FLCA+ Y I + C++ K + DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674
Query: 669 AVVFDGVLGGGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTEANEK 728
+VVF VVK+ R + NVGS Y+V + S V+I V P L+F++
Sbjct: 675 SVVF----ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734
Query: 729 KSYTVTFTTATGSAAPPSAEG--FGRIEWSDGKHVVGSPIAFSW 758
Y VTF + S G FG IEW+DG+HVV SP+A W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 756.5 bits (1952), Expect = 2.8e-217
Identity = 399/770 (51.82%), Postives = 522/770 (67.79%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKK-TYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTA 60
MA + LL S S A+ TYIVH+ P F H HWY SSL S+ S
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60
Query: 61 EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGL--DKNAD 120
+I+ Y+ V HGFS RLT+++A +L P +++V+PE LHTTR+P+FLGL A
Sbjct: 61 SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120
Query: 121 LYPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
L ES S+++IGV+DTGVWPE SFDD GLGPVP WKG+C + +F S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180
Query: 181 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
AR+F GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG V AS GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240
Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAM 300
+AR+AAYKVCW GC+ SDILAA + AV D V+V+S+S+GG + YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300
Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 360
++GI +S SAGN GP +++N +PW+TTVGAGT+DRDFPA V LG+GK SGVS+Y G
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360
Query: 361 PL-PGTLLPFIYAAN--ASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
L PG + P +Y + + + +LC+ G+L P V GKIV CDRG+N R KG +V+
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420
Query: 421 AGGIGMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPK------PTVTILF 480
GG+GM++AN +GE LVAD H+LPAT+V + GD IR+Y+ K PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480
Query: 481 EGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
+GT+LGI+P+PVVA+FS+RGPN TP++LKPD+IAPG+NILA W +GPSG+ D+RR
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540
Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKP 600
+FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT +G+ + D +TG
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600
Query: 601 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKY 660
S+ D+G+GHV P A++PGLVYD+T DY+NFLC NYT + I ++ RR CD ++
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660
Query: 661 S-VNDLNYPSFAVVFDGVLGGGSSVVKH-TRTLTNVG-SPGTYKVSISSETKLVKISVEP 720
V +LNYPSF+VVF G S + H RT+TNVG S Y++ I + ++VEP
Sbjct: 661 GHVGNLNYPSFSVVFQQY--GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEP 720
Query: 721 ESLSFTEANEKKSYTVTFTTATGSAAPPSAE-GFGRIEWSDGKHVVGSPI 754
E LSF +K S+ V T +P + G I WSDGK V SP+
Sbjct: 721 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767
BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match:
A0A6J1GEZ1 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE=3 SV=1)
HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 740/764 (96.86%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
M KP+WI LL CFFS P MA A KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1 MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60
Query: 61 AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
A+MIYAY VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61 ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120
Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
YPESTS SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360
Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420
Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
GMVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480
Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540
Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660
Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
AVVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720
Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KKSYTVTFTT TGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764
BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match:
A0A6J1IIG3 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3 SV=1)
HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 739/764 (96.73%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSM---AAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
M KP+ I LL CFFS P M AA KKTY+VHMAKYQMP +FEHHLHWYD+SLKSVSDT
Sbjct: 1 MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60
Query: 61 AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
A+MIYAY VVHGFSTRLTAEEA+RLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61 ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120
Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360
Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
L GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420
Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
GMVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480
Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540
Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660
Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
AVVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720
Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KKSYTVTFTTATGS+ PPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764
BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match:
A0A5D3CR08 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004280 PE=3 SV=1)
HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 713/762 (93.57%), Postives = 735/762 (96.46%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPD-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 60
MA P+W LLLC FS PSMA D KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1 MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60
Query: 61 MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 120
MIYAYNNVVHGFSTRLT EEAQRLE+QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61 MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120
Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
ES SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240
Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVA+GAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300
Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360
Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
GTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420
Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
VLANTAANGEELVADSHLLPATAV Q GDIIRKYL+S+PKPTVTILFEGTKLGI+PSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480
Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660
Query: 661 VFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKK 720
VFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720
Query: 721 SYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
SYTVTF T+ GSAAP SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761
BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match:
A0A1S3B0Y2 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=1)
HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 713/762 (93.57%), Postives = 735/762 (96.46%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPD-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 60
MA P+W LLLC FS PSMA D KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1 MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60
Query: 61 MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 120
MIYAYNNVVHGFSTRLT EEAQRLE+QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61 MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120
Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
ES SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240
Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVA+GAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300
Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360
Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
GTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420
Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
VLANTAANGEELVADSHLLPATAV Q GDIIRKYL+S+PKPTVTILFEGTKLGI+PSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480
Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660
Query: 661 VFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKK 720
VFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720
Query: 721 SYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
SYTVTF T+ GSAAP SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761
BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match:
A0A0A0KIG4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1)
HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 706/761 (92.77%), Postives = 733/761 (96.32%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
MA P+W+ LLLCFFS PSMA DKKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AEM
Sbjct: 1 MANPVWMFLLLCFFSVPSMAVGDKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAEM 60
Query: 61 IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 120
IYAYNNVVHGFSTRLTAEEAQRLE+QPGILAVVPEM YELHTTR+P+FLGLDKNA+LYPE
Sbjct: 61 IYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYPE 120
Query: 121 STSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYF 180
S SVSEVIIGVLDTG+ PESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKL+GAR+F
Sbjct: 121 SNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARFF 180
Query: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240
SKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARAR 240
Query: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGI 300
VAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGG+SDYYKDSVA+GAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 300
Query: 301 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPG 360
L+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLPG 360
Query: 361 TLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420
TLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGMV 420
Query: 421 LANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVV 480
LANTAANGEELVADSHLLPATAV Q GD IRKYL+S+P PTVTILFEGTKLGI+PSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPVV 480
Query: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540
AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540
Query: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 600
HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVDP 600
Query: 601 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVV 660
VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAVV
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAVV 660
Query: 661 FDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKS 720
F+GVLGG GSSVVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESLSFT AN+KKS
Sbjct: 661 FEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKKS 720
Query: 721 YTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
YTVTFTT T SAAP SAE FGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 YTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSWT 761
BLAST of Lcy04g015170 vs. NCBI nr
Match:
XP_038881674.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])
HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 730/761 (95.93%), Postives = 738/761 (96.98%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
MA PLWI LLLCFFS PSMAA KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSDTAEM
Sbjct: 1 MANPLWIFLLLCFFSVPSMAAAQKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEM 60
Query: 61 IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 120
IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNA+LYPE
Sbjct: 61 IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYPE 120
Query: 121 STSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYF 180
S SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+F
Sbjct: 121 SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 180
Query: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240
SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240
Query: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGI 300
VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGI 300
Query: 301 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPG 360
L+SCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKALPG 360
Query: 361 TLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420
TLLPFIYAANASNS NGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420
Query: 421 LANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVV 480
LANTAANGEELVADSHLLPATAV Q GDI+RKYLISEP PTVTILFEGTKLGI+PSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDIMRKYLISEPNPTVTILFEGTKLGIEPSPVV 480
Query: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540
AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGL IDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 540
Query: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 600
HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAY+NGQKIQD ATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYRNGQKIQDIATGKPSTPFDHGAGHVDP 600
Query: 601 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVV 660
VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLARRDFTCDS KKYSVNDLNYPSFAVV
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAVV 660
Query: 661 FDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKS 720
FDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANEKKS
Sbjct: 661 FDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKKS 720
Query: 721 YTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
YTVTFTT TGSAAPPSAEGFGRIEWSDGK VVGSPIAFSWT
Sbjct: 721 YTVTFTTVTGSAAPPSAEGFGRIEWSDGKQVVGSPIAFSWT 761
BLAST of Lcy04g015170 vs. NCBI nr
Match:
KAG6604148.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1430.6 bits (3702), Expect = 0.0e+00
Identity = 718/764 (93.98%), Postives = 741/764 (96.99%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
M KP+WI LL CFFS P MA A KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1 MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60
Query: 61 AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
A+MIYAY VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61 ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120
Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360
Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420
Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
GMVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480
Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540
Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660
Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
AVVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720
Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KKSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764
BLAST of Lcy04g015170 vs. NCBI nr
Match:
XP_023543760.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 716/763 (93.84%), Postives = 741/763 (97.12%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSM--AAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTA 60
M KP+W+ LL CFFS P M AA KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDTA
Sbjct: 1 MEKPVWVFLLFCFFSGPFMAAAAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDTA 60
Query: 61 EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLY 120
+MIYAY VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADLY
Sbjct: 61 DMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADLY 120
Query: 121 PESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180
PESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR
Sbjct: 121 PESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180
Query: 181 YFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240
+FS+GYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR
Sbjct: 181 FFSRGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240
Query: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300
ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK
Sbjct: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300
Query: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL 360
GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK L
Sbjct: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKSL 360
Query: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 420
PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIG
Sbjct: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIG 420
Query: 421 MVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSP 480
MVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PSP
Sbjct: 421 MVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPSP 480
Query: 481 VVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMS 540
VVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSMS
Sbjct: 481 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSMS 540
Query: 541 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHV 600
CPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHV
Sbjct: 541 CPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600
Query: 601 DPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFA 660
DPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSFA
Sbjct: 601 DPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSFA 660
Query: 661 VVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEK 720
VVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANEK
Sbjct: 661 VVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 720
Query: 721 KSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 763
BLAST of Lcy04g015170 vs. NCBI nr
Match:
KAG7034309.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 717/764 (93.85%), Postives = 741/764 (96.99%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
M KP+WI LL CFFS P MA A KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1 MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60
Query: 61 AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
A+MIYAY VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61 ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120
Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASG+FAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGSFAAME 300
Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360
Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420
Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
GMVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480
Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540
Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660
Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
AVVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720
Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KKSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764
BLAST of Lcy04g015170 vs. NCBI nr
Match:
XP_022950492.1 (subtilisin-like protease SBT1.7 [Cucurbita moschata])
HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 740/764 (96.86%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
M KP+WI LL CFFS P MA A KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1 MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60
Query: 61 AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
A+MIYAY VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61 ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120
Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
YPESTS SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360
Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420
Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
GMVLANTAANGEELVADSHLLPA+AV Q GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480
Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540
Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660
Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
AVVFDGVLGG GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720
Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
KKSYTVTFTT TGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764
BLAST of Lcy04g015170 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 547/759 (72.07%), Postives = 636/759 (83.79%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
++ + LLL F S ++ D+ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE+
Sbjct: 6 LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65
Query: 61 IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYP 120
+Y Y N +HGFSTRLT EEA L +QPG+++V+PE RYELHTTRTP FLGLD++ ADL+P
Sbjct: 66 LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125
Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
E+ S S+V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGAR+
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185
Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
F++GYE+T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RA
Sbjct: 186 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 245
Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
RVA YKVCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+G
Sbjct: 246 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 305
Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
IL+SCSAGNAGPS SLSN +PWITTVGAGTLDRDFPA LG+GKNF+GVSL++G+ LP
Sbjct: 306 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 365
Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
LLPFIYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM
Sbjct: 366 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 425
Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
+LANTAANGEELVAD+HLLPAT V + GDIIR Y+ ++P PT +I GT +G++PSPV
Sbjct: 426 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 485
Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
VAAFSSRGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSC
Sbjct: 486 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 545
Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
PHVSGLAAL+K HP+WSPAAIRSALMTTAY YK+G+ + D ATGKPSTPFDHGAGHV
Sbjct: 546 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVS 605
Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
P +A NPGL+YDLT +DYL FLCALNYTS QI S++RR++TCD K YSV DLNYPSFAV
Sbjct: 606 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665
Query: 661 VFDGVLGGGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKSYT 720
DGV K+TRT+T+VG GTY V ++SET VKISVEP L+F EANEKKSYT
Sbjct: 666 NVDGV-----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 725
Query: 721 VTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
VTFT S+ P + FG IEWSDGKHVVGSP+A SWT
Sbjct: 726 VTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757
BLAST of Lcy04g015170 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 811.6 bits (2095), Expect = 5.3e-235
Identity = 415/737 (56.31%), Postives = 533/737 (72.32%), Query Frame = 0
Query: 24 KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNNVVHGFSTRLTAEEAQR- 83
KKTYI+ + PESF H WY S L S S ++Y Y HGFS L + EA
Sbjct: 27 KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86
Query: 84 LESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVSEVIIGVLDTGVWPESKS 143
L S IL + + Y LHTTRTP+FLGL+ ++ +S + VIIGVLDTGVWPES+S
Sbjct: 87 LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146
Query: 144 FDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE-ATLGPIDESKESRSPR 203
FDDT + +PS WKGECESG++F + CN+KLIGAR FSKG++ A+ G +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206
Query: 204 DDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFSSDILAAI 263
D DGHGTHT+TTAAGS V NAS GYA+GTARGMA RARVA YKVCW+ GCF SDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266
Query: 264 EKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPSPYSLSNTSP 323
++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SCSAGN+GP+ S++N +P
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326
Query: 324 WITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGTLLPFIYAANASNSANGNLCM 383
W+ TVGAGTLDRDFPA+ +LG+GK +GVSLY G + L +Y N NS++ NLC+
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386
Query: 384 TGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPAT 443
G+L V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANTAA+GEELVADSHLLPA
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446
Query: 444 AVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDII 503
AV + GD++R+Y+ S+ KPT ++F+GT L ++PSPVVAAFSSRGPN++TP++LKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506
Query: 504 APGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAI 563
PGVNILAGWS ++GP+GL D RR FNI+SGTSMSCPH+SGLA L+K AHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566
Query: 564 RSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFL 623
+SALMTTAY + D A S P+ HG+GHVDP AL+PGLVYD++ ++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626
Query: 624 CALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVVFDGVLGGGSSVVKHTRTLTNV 683
C+L+YT I ++ +R S K LNYPSF+V+F GG VV++TR +TNV
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-----GGKRVVRYTREVTNV 686
Query: 684 GSPGT-YKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATGSAAPPSAEGFGRI 743
G+ + YKV+++ V ISV+P LSF EKK YTVTF + G + AE FG I
Sbjct: 687 GAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE-FGSI 746
Query: 744 EWSDGKHVVGSPIAFSW 758
WS+ +H V SP+AFSW
Sbjct: 747 TWSNPQHEVRSPVAFSW 751
BLAST of Lcy04g015170 vs. TAIR 10
Match:
AT5G51750.1 (subtilase 1.3 )
HSP 1 Score: 780.8 bits (2015), Expect = 1.0e-225
Identity = 397/750 (52.93%), Postives = 521/750 (69.47%), Query Frame = 0
Query: 24 KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 83
KKTY++HM K MP + +HL WY S + SV+ + ++Y Y HG + +
Sbjct: 34 KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93
Query: 84 LTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESTSVSEVIIGVL 143
LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++ ++ E + +V++GVL
Sbjct: 94 LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153
Query: 144 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYEATLGPID 203
DTG+WPES+SF+DTG+ PVP++W+G CE+G F NCNRK++GAR F +GYEA G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213
Query: 204 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 263
E E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273
Query: 264 FSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPS 323
FSSDIL+A+++AV D V VLS+SLGGG+S Y +DS++ F AME G+ +SCSAGN GP
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333
Query: 324 PYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 383
P SL+N SPWITTVGA T+DRDFPA V +G + F GVSLY+G+ + P +Y
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393
Query: 384 NASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 443
NAS+ + C+ G L VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453
Query: 444 ELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPN 503
ELVADSH+LPA AV + G +I++Y ++ K T ++ GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513
Query: 504 SITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALI 563
++ ++LKPD++APGVNILA W+ + PS L+ D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573
Query: 564 KGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLV 623
K HPDWSPAAI+SALMTTAY + + D + PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633
Query: 624 YDLTVDDYLNFLCALNYTSSQINSLARR-DFTCDSGKKYSVNDLNYPSFAVVFDGVLGGG 683
YD+ +Y FLC + + SQ+ + + TC + +LNYP+ + +F
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693
Query: 684 SSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATG 743
+ ++ RT+TNVG +YKVS+ S K ++V+P++L+FT ++K SYTVTF T
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 753
Query: 744 SAAPPSAEGFGRIEWSDGKHVVGSPIAFSW 758
P FG + W H V SP+ +W
Sbjct: 754 MKRPE----FGGLVWKSTTHKVRSPVIITW 776
BLAST of Lcy04g015170 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 772.3 bits (1993), Expect = 3.6e-223
Identity = 408/764 (53.40%), Postives = 517/764 (67.67%), Query Frame = 0
Query: 9 LLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 68
LLLCFFS S ++ ++YIVH+ + P F H +W+ S L+S+ + A ++Y+Y+
Sbjct: 15 LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74
Query: 69 NVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVS 128
VHGFS RL+ + L P +++V+P+ E+HTT TP FLG +N+ L+ S
Sbjct: 75 RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134
Query: 129 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE 188
+VI+GVLDTG+WPE SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194
Query: 189 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 248
+ +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254
Query: 249 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVASGAFAAMEKGIL 308
YK+CW GGC+ SDILAA+++AV D V+V+S+S+G G +Y+ DS+A GAF A GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314
Query: 309 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGT 368
+SCSAGN+GP+P + +N +PWI TVGA T+DR+F A GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374
Query: 369 LLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 428
L +Y S LC G L V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434
Query: 429 ANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLG-IQPSPVV 488
ANTA +GEEL ADSHL+PAT V GD IR Y+ + PT I F GT +G PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494
Query: 489 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 548
AAFSSRGPN +TP +LKPD+IAPGVNILAGW+ VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554
Query: 549 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 608
HVSGLAAL++ AHPDWSPAAI+SAL+TTAY +G+ I+D ATGK S F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614
Query: 609 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDSGKKYSVNDLNYPSF 668
ALNPGLVYD+ V +Y+ FLCA+ Y I + C++ K + DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674
Query: 669 AVVFDGVLGGGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTEANEK 728
+VVF VVK+ R + NVGS Y+V + S V+I V P L+F++
Sbjct: 675 SVVF----ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734
Query: 729 KSYTVTFTTATGSAAPPSAEG--FGRIEWSDGKHVVGSPIAFSW 758
Y VTF + S G FG IEW+DG+HVV SP+A W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769
BLAST of Lcy04g015170 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 756.5 bits (1952), Expect = 2.0e-218
Identity = 399/770 (51.82%), Postives = 522/770 (67.79%), Query Frame = 0
Query: 1 MAKPLWILLLLCFFSRPSMAAPDKK-TYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTA 60
MA + LL S S A+ TYIVH+ P F H HWY SSL S+ S
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60
Query: 61 EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGL--DKNAD 120
+I+ Y+ V HGFS RLT+++A +L P +++V+PE LHTTR+P+FLGL A
Sbjct: 61 SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120
Query: 121 LYPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
L ES S+++IGV+DTGVWPE SFDD GLGPVP WKG+C + +F S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180
Query: 181 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
AR+F GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG V AS GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240
Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAM 300
+AR+AAYKVCW GC+ SDILAA + AV D V+V+S+S+GG + YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300
Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 360
++GI +S SAGN GP +++N +PW+TTVGAGT+DRDFPA V LG+GK SGVS+Y G
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360
Query: 361 PL-PGTLLPFIYAAN--ASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
L PG + P +Y + + + +LC+ G+L P V GKIV CDRG+N R KG +V+
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420
Query: 421 AGGIGMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPK------PTVTILF 480
GG+GM++AN +GE LVAD H+LPAT+V + GD IR+Y+ K PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480
Query: 481 EGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
+GT+LGI+P+PVVA+FS+RGPN TP++LKPD+IAPG+NILA W +GPSG+ D+RR
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540
Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKP 600
+FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT +G+ + D +TG
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600
Query: 601 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKY 660
S+ D+G+GHV P A++PGLVYD+T DY+NFLC NYT + I ++ RR CD ++
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660
Query: 661 S-VNDLNYPSFAVVFDGVLGGGSSVVKH-TRTLTNVG-SPGTYKVSISSETKLVKISVEP 720
V +LNYPSF+VVF G S + H RT+TNVG S Y++ I + ++VEP
Sbjct: 661 GHVGNLNYPSFSVVFQQY--GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEP 720
Query: 721 ESLSFTEANEKKSYTVTFTTATGSAAPPSAE-GFGRIEWSDGKHVVGSPI 754
E LSF +K S+ V T +P + G I WSDGK V SP+
Sbjct: 721 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
O65351 | 0.0e+00 | 72.07 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
Q9ZUF6 | 7.5e-234 | 56.31 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
Q9FLI4 | 1.4e-224 | 52.93 | Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... | [more] |
Q9LVJ1 | 5.0e-222 | 53.40 | Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... | [more] |
Q9LUM3 | 2.8e-217 | 51.82 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GEZ1 | 0.0e+00 | 93.72 | subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE... | [more] |
A0A6J1IIG3 | 0.0e+00 | 93.72 | subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3... | [more] |
A0A5D3CR08 | 0.0e+00 | 93.57 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
A0A1S3B0Y2 | 0.0e+00 | 93.57 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=... | [more] |
A0A0A0KIG4 | 0.0e+00 | 92.77 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_038881674.1 | 0.0e+00 | 95.93 | subtilisin-like protease SBT1.7 [Benincasa hispida] | [more] |
KAG6604148.1 | 0.0e+00 | 93.98 | Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_023543760.1 | 0.0e+00 | 93.84 | subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo] | [more] |
KAG7034309.1 | 0.0e+00 | 93.85 | Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosp... | [more] |
XP_022950492.1 | 0.0e+00 | 93.72 | subtilisin-like protease SBT1.7 [Cucurbita moschata] | [more] |