Lcy04g015170 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g015170
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionsubtilisin-like protease SBT1.7
LocationChr04: 47493476 .. 47496568 (-)
RNA-Seq ExpressionLcy04g015170
SyntenyLcy04g015170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAATTAATTTCATTCAATGAATCTGAAAAACAAAACAAGATTCAAATTACATACCTCTTTAATTTTTGTATTGAATTCGTGGAGTTTTATTGGGCAGTGGGGAAAAAGCAAAAAAAAAAAAAAATGTATTAAGCAGTAGAAAGGTGAGAGTAGGAGGGAAAGAGAAGAAGAAAAAGCGGTTGTACACGTGATGAAAGGAAGCAGTAACTCCTCCGCCAGTAAGCCCTCCACGTTCGTTGAGAGCTGTCGCTAGGTCCTCTTCCGTTTTGGCTCCACGCAATTATTCACTCAACAAGACAACCACCCACCACCTCCCCATTCTCTCTCCTGTCTCCTCTGCCTCTCCGTTTTATAATACTCCACGCCCTGCTTCTTTTACTTTGATGCTCTCTCCTTCTCATCACTGCAGATTCAATGGCGAAGCCGCTTTGGATTCTTCTACTTCTCTGTTTCTTCTCTAGGCCGTCAATGGCTGCTCCAGACAAGAAGACTTACATCGTGCATATGGCCAAGTACCAAATGCCGGAGAGTTTTGAGCACCATTTGCACTGGTATGACTCGTCCCTCAAATCCGTCTCCGATACGGCGGAGATGATCTATGCTTACAACAACGTCGTTCATGGGTTTTCAACCAGATTGACGGCGGAGGAAGCTCAGCGCCTGGAGTCCCAACCTGGGATTTTGGCTGTGGTGCCGGAGATGAGATATGAACTTCATACCACTCGTACTCCTCAGTTTCTTGGACTTGACAAGAATGCAGATCTGTACCCTGAATCGACCTCAGTATCGGAGGTGATCATCGGAGTTTTGGATACCGGGGTTTGGCCGGAGAGTAAGAGCTTCGACGATACTGGGCTTGGACCGGTGCCGAGTAGCTGGAAGGGTGAGTGTGAATCCGGTACTAATTTCAGTGCGTCGAACTGTAACAGGAAGCTGATCGGAGCGAGGTATTTTTCCAAGGGCTACGAGGCGACTCTCGGTCCGATCGATGAATCCAAAGAATCGAGATCTCCGAGAGACGACGACGGCCATGGAACCCACACCGCAACCACTGCCGCCGGTTCTGTAGTGGAAAACGCCAGCCTATTTGGCTACGCCTCTGGCACCGCCCGTGGGATGGCTGCACGTGCGAGGGTCGCTGCCTATAAGGTCTGTTGGGCCGGCGGATGTTTCAGCTCCGACATCTTGGCAGCCATTGAAAAAGCTGTGGAGGACAATGTTAATGTCCTTTCCATGTCGCTCGGCGGTGGAATATCCGACTATTACAAAGACAGCGTTGCCTCCGGAGCCTTCGCCGCCATGGAGAAAGGCATCCTCATTTCTTGCTCCGCCGGAAATGCGGGCCCCAGCCCTTACAGCTTGTCGAATACGTCTCCATGGATCACAACCGTCGGTGCCGGAACATTAGATCGCGATTTTCCGGCGTACGTCAGTCTCGGCGACGGCAAAAACTTCTCCGGTGTTTCGCTCTATCGAGGCAAGCCATTGCCGGGAACCTTGTTGCCTTTTATTTACGCCGCTAATGCGAGTAACTCTGCTAATGGCAATTTGTGTATGACCGGTACTTTGATCCCTGAAAAAGTTGCCGGAAAAATCGTGTTCTGTGACCGAGGTGTAAATCCTAGGGTTCAGAAAGGGGCGGTCGTCAAAGCCGCAGGTGGAATCGGAATGGTGTTGGCCAACACCGCCGCAAACGGAGAAGAGTTGGTGGCCGACTCTCATCTTCTGCCGGCCACGGCGGTGGATCAGAATTTCGGCGACATTATACGGAAGTATCTGATTTCGGAGCCGAAACCGACGGTGACGATCTTATTCGAAGGTACAAAATTGGGGATCCAACCGTCGCCGGTGGTGGCGGCTTTCAGTTCCCGGGGACCCAATTCGATCACTCCTCAGTTGCTGAAGCCCGACATAATCGCTCCTGGTGTCAACATTTTAGCTGGATGGTCAAAATCAGTGGGGCCGAGTGGTTTAGCCATTGATGACAGAAGAGTGGATTTCAACATTATCTCTGGCACCTCCATGTCCTGCCCCCATGTGAGCGGTCTCGCCGCCCTAATCAAAGGGGCTCATCCCGACTGGAGCCCGGCGGCGATTCGTTCGGCGTTGATGACGACGGCCTACACAGCCTACAAAAACGGCCAGAAGATCCAAGATACCGCCACCGGAAAACCGTCCACGCCATTCGATCACGGAGCCGGACACGTCGATCCAGTATCAGCCCTCAATCCGGGTCTTGTTTACGATCTAACGGTGGACGATTACCTGAACTTCCTCTGTGCACTTAACTACACTTCGTCGCAGATCAACTCACTGGCGAGGAGAGATTTCACTTGCGACTCCGGGAAGAAGTACAGTGTCAACGATCTCAACTACCCTTCTTTCGCCGTCGTTTTCGACGGCGTATTAGGCGGAGGTTCCAGTGTAGTCAAGCACACCAGAACTCTTACAAACGTCGGCTCTCCAGGAACATACAAAGTCTCAATCTCGTCGGAGACCAAGCTGGTGAAAATCTCCGTCGAGCCAGAATCTTTAAGCTTCACCGAAGCCAATGAGAAGAAGTCGTACACAGTCACATTCACCACCGCAACTGGTTCGGCGGCGCCGCCGAGCGCGGAGGGGTTCGGTAGAATTGAGTGGTCGGACGGGAAGCACGTGGTGGGAAGTCCGATTGCGTTTAGTTGGACGTAGGAGCCAATGAAGAAGATGGGTCTTGTTGAATTTCCATGGCGAATGCCTCGATCGAACCTGAAAGTTGAAAGTTAATGTAATATTTGTTTGTCGAATTTCCAGCGAGAAATAGAGAGCTGGATTTATTGTTTTAACGTTTCGTTCTTCCAGAAAAAGGGAAAGGAAAAAAGAAAAAAAAAAAACGATAATAATAGGAAATCGCGTTTAGTGCTTTATTTTGCATTTTCTGTGTACAGAATTGATAATTGCTCGTGTGTCTGTTGCACTCGCTTCAATTGCTGTGGAACACAAGTTGATGTTCAAGTTGGGTTGCATTTCTCTCTTTTTCTTTGCCTAAGCGTATCTCTCCATTCATTCATATTTTACTTCATTTTTATTTCTTCCAAATTGAGTAATATT

mRNA sequence

GTTAAATTAATTTCATTCAATGAATCTGAAAAACAAAACAAGATTCAAATTACATACCTCTTTAATTTTTGTATTGAATTCGTGGAGTTTTATTGGGCAGTGGGGAAAAAGCAAAAAAAAAAAAAAATGTATTAAGCAGTAGAAAGGTGAGAGTAGGAGGGAAAGAGAAGAAGAAAAAGCGGTTGTACACGTGATGAAAGGAAGCAGTAACTCCTCCGCCAGTAAGCCCTCCACGTTCGTTGAGAGCTGTCGCTAGGTCCTCTTCCGTTTTGGCTCCACGCAATTATTCACTCAACAAGACAACCACCCACCACCTCCCCATTCTCTCTCCTGTCTCCTCTGCCTCTCCGTTTTATAATACTCCACGCCCTGCTTCTTTTACTTTGATGCTCTCTCCTTCTCATCACTGCAGATTCAATGGCGAAGCCGCTTTGGATTCTTCTACTTCTCTGTTTCTTCTCTAGGCCGTCAATGGCTGCTCCAGACAAGAAGACTTACATCGTGCATATGGCCAAGTACCAAATGCCGGAGAGTTTTGAGCACCATTTGCACTGGTATGACTCGTCCCTCAAATCCGTCTCCGATACGGCGGAGATGATCTATGCTTACAACAACGTCGTTCATGGGTTTTCAACCAGATTGACGGCGGAGGAAGCTCAGCGCCTGGAGTCCCAACCTGGGATTTTGGCTGTGGTGCCGGAGATGAGATATGAACTTCATACCACTCGTACTCCTCAGTTTCTTGGACTTGACAAGAATGCAGATCTGTACCCTGAATCGACCTCAGTATCGGAGGTGATCATCGGAGTTTTGGATACCGGGGTTTGGCCGGAGAGTAAGAGCTTCGACGATACTGGGCTTGGACCGGTGCCGAGTAGCTGGAAGGGTGAGTGTGAATCCGGTACTAATTTCAGTGCGTCGAACTGTAACAGGAAGCTGATCGGAGCGAGGTATTTTTCCAAGGGCTACGAGGCGACTCTCGGTCCGATCGATGAATCCAAAGAATCGAGATCTCCGAGAGACGACGACGGCCATGGAACCCACACCGCAACCACTGCCGCCGGTTCTGTAGTGGAAAACGCCAGCCTATTTGGCTACGCCTCTGGCACCGCCCGTGGGATGGCTGCACGTGCGAGGGTCGCTGCCTATAAGGTCTGTTGGGCCGGCGGATGTTTCAGCTCCGACATCTTGGCAGCCATTGAAAAAGCTGTGGAGGACAATGTTAATGTCCTTTCCATGTCGCTCGGCGGTGGAATATCCGACTATTACAAAGACAGCGTTGCCTCCGGAGCCTTCGCCGCCATGGAGAAAGGCATCCTCATTTCTTGCTCCGCCGGAAATGCGGGCCCCAGCCCTTACAGCTTGTCGAATACGTCTCCATGGATCACAACCGTCGGTGCCGGAACATTAGATCGCGATTTTCCGGCGTACGTCAGTCTCGGCGACGGCAAAAACTTCTCCGGTGTTTCGCTCTATCGAGGCAAGCCATTGCCGGGAACCTTGTTGCCTTTTATTTACGCCGCTAATGCGAGTAACTCTGCTAATGGCAATTTGTGTATGACCGGTACTTTGATCCCTGAAAAAGTTGCCGGAAAAATCGTGTTCTGTGACCGAGGTGTAAATCCTAGGGTTCAGAAAGGGGCGGTCGTCAAAGCCGCAGGTGGAATCGGAATGGTGTTGGCCAACACCGCCGCAAACGGAGAAGAGTTGGTGGCCGACTCTCATCTTCTGCCGGCCACGGCGGTGGATCAGAATTTCGGCGACATTATACGGAAGTATCTGATTTCGGAGCCGAAACCGACGGTGACGATCTTATTCGAAGGTACAAAATTGGGGATCCAACCGTCGCCGGTGGTGGCGGCTTTCAGTTCCCGGGGACCCAATTCGATCACTCCTCAGTTGCTGAAGCCCGACATAATCGCTCCTGGTGTCAACATTTTAGCTGGATGGTCAAAATCAGTGGGGCCGAGTGGTTTAGCCATTGATGACAGAAGAGTGGATTTCAACATTATCTCTGGCACCTCCATGTCCTGCCCCCATGTGAGCGGTCTCGCCGCCCTAATCAAAGGGGCTCATCCCGACTGGAGCCCGGCGGCGATTCGTTCGGCGTTGATGACGACGGCCTACACAGCCTACAAAAACGGCCAGAAGATCCAAGATACCGCCACCGGAAAACCGTCCACGCCATTCGATCACGGAGCCGGACACGTCGATCCAGTATCAGCCCTCAATCCGGGTCTTGTTTACGATCTAACGGTGGACGATTACCTGAACTTCCTCTGTGCACTTAACTACACTTCGTCGCAGATCAACTCACTGGCGAGGAGAGATTTCACTTGCGACTCCGGGAAGAAGTACAGTGTCAACGATCTCAACTACCCTTCTTTCGCCGTCGTTTTCGACGGCGTATTAGGCGGAGGTTCCAGTGTAGTCAAGCACACCAGAACTCTTACAAACGTCGGCTCTCCAGGAACATACAAAGTCTCAATCTCGTCGGAGACCAAGCTGGTGAAAATCTCCGTCGAGCCAGAATCTTTAAGCTTCACCGAAGCCAATGAGAAGAAGTCGTACACAGTCACATTCACCACCGCAACTGGTTCGGCGGCGCCGCCGAGCGCGGAGGGGTTCGGTAGAATTGAGTGGTCGGACGGGAAGCACGTGGTGGGAAGTCCGATTGCGTTTAGTTGGACGTAGGAGCCAATGAAGAAGATGGGTCTTGTTGAATTTCCATGGCGAATGCCTCGATCGAACCTGAAAGTTGAAAGTTAATGTAATATTTGTTTGTCGAATTTCCAGCGAGAAATAGAGAGCTGGATTTATTGTTTTAACGTTTCGTTCTTCCAGAAAAAGGGAAAGGAAAAAAGAAAAAAAAAAAACGATAATAATAGGAAATCGCGTTTAGTGCTTTATTTTGCATTTTCTGTGTACAGAATTGATAATTGCTCGTGTGTCTGTTGCACTCGCTTCAATTGCTGTGGAACACAAGTTGATGTTCAAGTTGGGTTGCATTTCTCTCTTTTTCTTTGCCTAAGCGTATCTCTCCATTCATTCATATTTTACTTCATTTTTATTTCTTCCAAATTGAGTAATATT

Coding sequence (CDS)

ATGGCGAAGCCGCTTTGGATTCTTCTACTTCTCTGTTTCTTCTCTAGGCCGTCAATGGCTGCTCCAGACAAGAAGACTTACATCGTGCATATGGCCAAGTACCAAATGCCGGAGAGTTTTGAGCACCATTTGCACTGGTATGACTCGTCCCTCAAATCCGTCTCCGATACGGCGGAGATGATCTATGCTTACAACAACGTCGTTCATGGGTTTTCAACCAGATTGACGGCGGAGGAAGCTCAGCGCCTGGAGTCCCAACCTGGGATTTTGGCTGTGGTGCCGGAGATGAGATATGAACTTCATACCACTCGTACTCCTCAGTTTCTTGGACTTGACAAGAATGCAGATCTGTACCCTGAATCGACCTCAGTATCGGAGGTGATCATCGGAGTTTTGGATACCGGGGTTTGGCCGGAGAGTAAGAGCTTCGACGATACTGGGCTTGGACCGGTGCCGAGTAGCTGGAAGGGTGAGTGTGAATCCGGTACTAATTTCAGTGCGTCGAACTGTAACAGGAAGCTGATCGGAGCGAGGTATTTTTCCAAGGGCTACGAGGCGACTCTCGGTCCGATCGATGAATCCAAAGAATCGAGATCTCCGAGAGACGACGACGGCCATGGAACCCACACCGCAACCACTGCCGCCGGTTCTGTAGTGGAAAACGCCAGCCTATTTGGCTACGCCTCTGGCACCGCCCGTGGGATGGCTGCACGTGCGAGGGTCGCTGCCTATAAGGTCTGTTGGGCCGGCGGATGTTTCAGCTCCGACATCTTGGCAGCCATTGAAAAAGCTGTGGAGGACAATGTTAATGTCCTTTCCATGTCGCTCGGCGGTGGAATATCCGACTATTACAAAGACAGCGTTGCCTCCGGAGCCTTCGCCGCCATGGAGAAAGGCATCCTCATTTCTTGCTCCGCCGGAAATGCGGGCCCCAGCCCTTACAGCTTGTCGAATACGTCTCCATGGATCACAACCGTCGGTGCCGGAACATTAGATCGCGATTTTCCGGCGTACGTCAGTCTCGGCGACGGCAAAAACTTCTCCGGTGTTTCGCTCTATCGAGGCAAGCCATTGCCGGGAACCTTGTTGCCTTTTATTTACGCCGCTAATGCGAGTAACTCTGCTAATGGCAATTTGTGTATGACCGGTACTTTGATCCCTGAAAAAGTTGCCGGAAAAATCGTGTTCTGTGACCGAGGTGTAAATCCTAGGGTTCAGAAAGGGGCGGTCGTCAAAGCCGCAGGTGGAATCGGAATGGTGTTGGCCAACACCGCCGCAAACGGAGAAGAGTTGGTGGCCGACTCTCATCTTCTGCCGGCCACGGCGGTGGATCAGAATTTCGGCGACATTATACGGAAGTATCTGATTTCGGAGCCGAAACCGACGGTGACGATCTTATTCGAAGGTACAAAATTGGGGATCCAACCGTCGCCGGTGGTGGCGGCTTTCAGTTCCCGGGGACCCAATTCGATCACTCCTCAGTTGCTGAAGCCCGACATAATCGCTCCTGGTGTCAACATTTTAGCTGGATGGTCAAAATCAGTGGGGCCGAGTGGTTTAGCCATTGATGACAGAAGAGTGGATTTCAACATTATCTCTGGCACCTCCATGTCCTGCCCCCATGTGAGCGGTCTCGCCGCCCTAATCAAAGGGGCTCATCCCGACTGGAGCCCGGCGGCGATTCGTTCGGCGTTGATGACGACGGCCTACACAGCCTACAAAAACGGCCAGAAGATCCAAGATACCGCCACCGGAAAACCGTCCACGCCATTCGATCACGGAGCCGGACACGTCGATCCAGTATCAGCCCTCAATCCGGGTCTTGTTTACGATCTAACGGTGGACGATTACCTGAACTTCCTCTGTGCACTTAACTACACTTCGTCGCAGATCAACTCACTGGCGAGGAGAGATTTCACTTGCGACTCCGGGAAGAAGTACAGTGTCAACGATCTCAACTACCCTTCTTTCGCCGTCGTTTTCGACGGCGTATTAGGCGGAGGTTCCAGTGTAGTCAAGCACACCAGAACTCTTACAAACGTCGGCTCTCCAGGAACATACAAAGTCTCAATCTCGTCGGAGACCAAGCTGGTGAAAATCTCCGTCGAGCCAGAATCTTTAAGCTTCACCGAAGCCAATGAGAAGAAGTCGTACACAGTCACATTCACCACCGCAACTGGTTCGGCGGCGCCGCCGAGCGCGGAGGGGTTCGGTAGAATTGAGTGGTCGGACGGGAAGCACGTGGTGGGAAGTCCGATTGCGTTTAGTTGGACGTAG

Protein sequence

MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVVFDGVLGGGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT
Homology
BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 547/759 (72.07%), Postives = 636/759 (83.79%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
           ++   + LLL   F   S ++ D+ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE+
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65

Query: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYP 120
           +Y Y N +HGFSTRLT EEA  L +QPG+++V+PE RYELHTTRTP FLGLD++ ADL+P
Sbjct: 66  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125

Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
           E+ S S+V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGAR+
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           F++GYE+T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RA
Sbjct: 186 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 245

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
           RVA YKVCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+G
Sbjct: 246 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 305

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ LP
Sbjct: 306 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 365

Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
             LLPFIYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM
Sbjct: 366 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 425

Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
           +LANTAANGEELVAD+HLLPAT V +  GDIIR Y+ ++P PT +I   GT +G++PSPV
Sbjct: 426 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 485

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSC
Sbjct: 486 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 545

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
           PHVSGLAAL+K  HP+WSPAAIRSALMTTAY  YK+G+ + D ATGKPSTPFDHGAGHV 
Sbjct: 546 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVS 605

Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
           P +A NPGL+YDLT +DYL FLCALNYTS QI S++RR++TCD  K YSV DLNYPSFAV
Sbjct: 606 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

Query: 661 VFDGVLGGGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKSYT 720
             DGV        K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F EANEKKSYT
Sbjct: 666 NVDGV-----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 725

Query: 721 VTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           VTFT    S+ P  +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 726 VTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 7.5e-234
Identity = 415/737 (56.31%), Postives = 533/737 (72.32%), Query Frame = 0

Query: 24  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNNVVHGFSTRLTAEEAQR- 83
           KKTYI+ +     PESF  H  WY S L S S    ++Y Y    HGFS  L + EA   
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 84  LESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVSEVIIGVLDTGVWPESKS 143
           L S   IL +  +  Y LHTTRTP+FLGL+    ++   +S + VIIGVLDTGVWPES+S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 144 FDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE-ATLGPIDESKESRSPR 203
           FDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 204 DDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFSSDILAAI 263
           D DGHGTHT+TTAAGS V NAS  GYA+GTARGMA RARVA YKVCW+ GCF SDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 264 EKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPSPYSLSNTSP 323
           ++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SCSAGN+GP+  S++N +P
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 324 WITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGTLLPFIYAANASNSANGNLCM 383
           W+ TVGAGTLDRDFPA+ +LG+GK  +GVSLY G  +    L  +Y  N  NS++ NLC+
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 384 TGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPAT 443
            G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANTAA+GEELVADSHLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 444 AVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDII 503
           AV +  GD++R+Y+ S+ KPT  ++F+GT L ++PSPVVAAFSSRGPN++TP++LKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 504 APGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAI 563
            PGVNILAGWS ++GP+GL  D RR  FNI+SGTSMSCPH+SGLA L+K AHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 564 RSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFL 623
           +SALMTTAY        + D A    S P+ HG+GHVDP  AL+PGLVYD++ ++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 624 CALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVVFDGVLGGGSSVVKHTRTLTNV 683
           C+L+YT   I ++ +R     S K      LNYPSF+V+F     GG  VV++TR +TNV
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-----GGKRVVRYTREVTNV 686

Query: 684 GSPGT-YKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATGSAAPPSAEGFGRI 743
           G+  + YKV+++     V ISV+P  LSF    EKK YTVTF +  G +    AE FG I
Sbjct: 687 GAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE-FGSI 746

Query: 744 EWSDGKHVVGSPIAFSW 758
            WS+ +H V SP+AFSW
Sbjct: 747 TWSNPQHEVRSPVAFSW 751

BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 1.4e-224
Identity = 397/750 (52.93%), Postives = 521/750 (69.47%), Query Frame = 0

Query: 24  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 83
           KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y Y    HG + +
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 84  LTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESTSVSEVIIGVL 143
           LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++     ++ E  +  +V++GVL
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153

Query: 144 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYEATLGPID 203
           DTG+WPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F +GYEA  G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213

Query: 204 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 263
           E  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273

Query: 264 FSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPS 323
           FSSDIL+A+++AV D V VLS+SLGGG+S Y +DS++   F AME G+ +SCSAGN GP 
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333

Query: 324 PYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 383
           P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +       P +Y   
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393

Query: 384 NASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 443
           NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453

Query: 444 ELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPN 503
           ELVADSH+LPA AV +  G +I++Y ++  K T ++   GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513

Query: 504 SITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALI 563
            ++ ++LKPD++APGVNILA W+  + PS L+ D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573

Query: 564 KGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLV 623
           K  HPDWSPAAI+SALMTTAY      + + D +   PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633

Query: 624 YDLTVDDYLNFLCALNYTSSQINSLARR-DFTCDSGKKYSVNDLNYPSFAVVFDGVLGGG 683
           YD+   +Y  FLC  + + SQ+    +  + TC      +  +LNYP+ + +F       
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693

Query: 684 SSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATG 743
           +  ++  RT+TNVG    +YKVS+ S  K   ++V+P++L+FT  ++K SYTVTF T   
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 753

Query: 744 SAAPPSAEGFGRIEWSDGKHVVGSPIAFSW 758
              P     FG + W    H V SP+  +W
Sbjct: 754 MKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 5.0e-222
Identity = 408/764 (53.40%), Postives = 517/764 (67.67%), Query Frame = 0

Query: 9   LLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 68
           LLLCFFS  S ++   ++YIVH+ +   P  F  H +W+ S L+S+  +   A ++Y+Y+
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74

Query: 69  NVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVS 128
             VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  S    
Sbjct: 75  RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134

Query: 129 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE 188
           +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY 
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194

Query: 189 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 248
                  +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254

Query: 249 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVASGAFAAMEKGIL 308
           YK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314

Query: 309 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGT 368
           +SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374

Query: 369 LLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 428
            L  +Y    S      LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434

Query: 429 ANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLG-IQPSPVV 488
           ANTA +GEEL ADSHL+PAT V    GD IR Y+ +   PT  I F GT +G   PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494

Query: 489 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 548
           AAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554

Query: 549 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 608
           HVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ I+D ATGK S  F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614

Query: 609 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDSGKKYSVNDLNYPSF 668
             ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C++ K  +  DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674

Query: 669 AVVFDGVLGGGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTEANEK 728
           +VVF         VVK+ R + NVGS     Y+V + S    V+I V P  L+F++    
Sbjct: 675 SVVF----ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734

Query: 729 KSYTVTFTTATGSAAPPSAEG--FGRIEWSDGKHVVGSPIAFSW 758
             Y VTF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lcy04g015170 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.8e-217
Identity = 399/770 (51.82%), Postives = 522/770 (67.79%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKK-TYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTA 60
           MA   +   LL   S  S A+     TYIVH+     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGL--DKNAD 120
            +I+ Y+ V HGFS RLT+++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 121 LYPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
           L  ES   S+++IGV+DTGVWPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 181 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
           AR+F  GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAM 300
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG +  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 360
           ++GI +S SAGN GP   +++N +PW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 361 PL-PGTLLPFIYAAN--ASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
            L PG + P +Y  +    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 421 AGGIGMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPK------PTVTILF 480
            GG+GM++AN   +GE LVAD H+LPAT+V  + GD IR+Y+    K      PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 481 EGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
           +GT+LGI+P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKP 600
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 601 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKY 660
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 661 S-VNDLNYPSFAVVFDGVLGGGSSVVKH-TRTLTNVG-SPGTYKVSISSETKLVKISVEP 720
             V +LNYPSF+VVF     G S +  H  RT+TNVG S   Y++ I    +   ++VEP
Sbjct: 661 GHVGNLNYPSFSVVFQQY--GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEP 720

Query: 721 ESLSFTEANEKKSYTVTFTTATGSAAPPSAE-GFGRIEWSDGKHVVGSPI 754
           E LSF    +K S+ V   T     +P +     G I WSDGK  V SP+
Sbjct: 721 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match: A0A6J1GEZ1 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE=3 SV=1)

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 740/764 (96.86%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
           M KP+WI LL CFFS P MA   A  KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPESTS SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
           GMVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
           AVVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KKSYTVTFTT TGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match: A0A6J1IIG3 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3 SV=1)

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 739/764 (96.73%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSM---AAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
           M KP+ I LL CFFS P M   AA  KKTY+VHMAKYQMP +FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
           A+MIYAY  VVHGFSTRLTAEEA+RLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360

Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           L GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
           GMVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
           AVVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KKSYTVTFTTATGS+ PPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match: A0A5D3CR08 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004280 PE=3 SV=1)

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 713/762 (93.57%), Postives = 735/762 (96.46%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPD-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 60
           MA P+W  LLLC FS PSMA  D KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 120
           MIYAYNNVVHGFSTRLT EEAQRLE+QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
           ES SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVA+GAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
           VLANTAANGEELVADSHLLPATAV Q  GDIIRKYL+S+PKPTVTILFEGTKLGI+PSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKK 720
           VFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           SYTVTF T+ GSAAP SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match: A0A1S3B0Y2 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=1)

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 713/762 (93.57%), Postives = 735/762 (96.46%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPD-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 60
           MA P+W  LLLC FS PSMA  D KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 120
           MIYAYNNVVHGFSTRLT EEAQRLE+QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
           ES SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVA+GAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
           VLANTAANGEELVADSHLLPATAV Q  GDIIRKYL+S+PKPTVTILFEGTKLGI+PSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKK 720
           VFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           SYTVTF T+ GSAAP SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Lcy04g015170 vs. ExPASy TrEMBL
Match: A0A0A0KIG4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 706/761 (92.77%), Postives = 733/761 (96.32%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
           MA P+W+ LLLCFFS PSMA  DKKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AEM
Sbjct: 1   MANPVWMFLLLCFFSVPSMAVGDKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAEM 60

Query: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 120
           IYAYNNVVHGFSTRLTAEEAQRLE+QPGILAVVPEM YELHTTR+P+FLGLDKNA+LYPE
Sbjct: 61  IYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYPE 120

Query: 121 STSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYF 180
           S SVSEVIIGVLDTG+ PESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKL+GAR+F
Sbjct: 121 SNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARFF 180

Query: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240
           SKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARAR 240

Query: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGI 300
           VAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGG+SDYYKDSVA+GAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 300

Query: 301 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPG 360
           L+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLPG 360

Query: 361 TLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420
           TLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGMV 420

Query: 421 LANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVV 480
           LANTAANGEELVADSHLLPATAV Q  GD IRKYL+S+P PTVTILFEGTKLGI+PSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPVV 480

Query: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540
           AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540

Query: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 600
           HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVDP 600

Query: 601 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVV 660
           VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCDS KKYSVNDLNYPSFAVV
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAVV 660

Query: 661 FDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKS 720
           F+GVLGG   GSSVVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESLSFT AN+KKS
Sbjct: 661 FEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKKS 720

Query: 721 YTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           YTVTFTT T SAAP SAE FGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 YTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Lcy04g015170 vs. NCBI nr
Match: XP_038881674.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 730/761 (95.93%), Postives = 738/761 (96.98%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
           MA PLWI LLLCFFS PSMAA  KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSDTAEM
Sbjct: 1   MANPLWIFLLLCFFSVPSMAAAQKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEM 60

Query: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 120
           IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNA+LYPE
Sbjct: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYPE 120

Query: 121 STSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYF 180
           S SVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR+F
Sbjct: 121 SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 180

Query: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240
           SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240

Query: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGI 300
           VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGI 300

Query: 301 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPG 360
           L+SCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKALPG 360

Query: 361 TLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420
           TLLPFIYAANASNS NGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420

Query: 421 LANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVV 480
           LANTAANGEELVADSHLLPATAV Q  GDI+RKYLISEP PTVTILFEGTKLGI+PSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDIMRKYLISEPNPTVTILFEGTKLGIEPSPVV 480

Query: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540
           AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGL IDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 540

Query: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 600
           HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAY+NGQKIQD ATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYRNGQKIQDIATGKPSTPFDHGAGHVDP 600

Query: 601 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVV 660
           VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLARRDFTCDS KKYSVNDLNYPSFAVV
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAVV 660

Query: 661 FDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKS 720
           FDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANEKKS
Sbjct: 661 FDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKKS 720

Query: 721 YTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           YTVTFTT TGSAAPPSAEGFGRIEWSDGK VVGSPIAFSWT
Sbjct: 721 YTVTFTTVTGSAAPPSAEGFGRIEWSDGKQVVGSPIAFSWT 761

BLAST of Lcy04g015170 vs. NCBI nr
Match: KAG6604148.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1430.6 bits (3702), Expect = 0.0e+00
Identity = 718/764 (93.98%), Postives = 741/764 (96.99%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
           M KP+WI LL CFFS P MA   A  KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
           GMVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
           AVVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KKSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Lcy04g015170 vs. NCBI nr
Match: XP_023543760.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 716/763 (93.84%), Postives = 741/763 (97.12%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSM--AAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTA 60
           M KP+W+ LL CFFS P M  AA  KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDTA
Sbjct: 1   MEKPVWVFLLFCFFSGPFMAAAAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDTA 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLY 120
           +MIYAY  VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADLY
Sbjct: 61  DMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADLY 120

Query: 121 PESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180
           PESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR
Sbjct: 121 PESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180

Query: 181 YFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240
           +FS+GYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR
Sbjct: 181 FFSRGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240

Query: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300
           ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK
Sbjct: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300

Query: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL 360
           GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK L
Sbjct: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKSL 360

Query: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 420
           PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIG
Sbjct: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIG 420

Query: 421 MVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSP 480
           MVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PSP
Sbjct: 421 MVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPSP 480

Query: 481 VVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMS 540
           VVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSMS
Sbjct: 481 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSMS 540

Query: 541 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHV 600
           CPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGHV
Sbjct: 541 CPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600

Query: 601 DPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFA 660
           DPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSFA
Sbjct: 601 DPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSFA 660

Query: 661 VVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEK 720
           VVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANEK
Sbjct: 661 VVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 720

Query: 721 KSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 763

BLAST of Lcy04g015170 vs. NCBI nr
Match: KAG7034309.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 717/764 (93.85%), Postives = 741/764 (96.99%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
           M KP+WI LL CFFS P MA   A  KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPESTS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASG+FAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGSFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
           GMVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
           AVVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KKSYTVTFTTATGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Lcy04g015170 vs. NCBI nr
Match: XP_022950492.1 (subtilisin-like protease SBT1.7 [Cucurbita moschata])

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 716/764 (93.72%), Postives = 740/764 (96.86%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMA---APDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 60
           M KP+WI LL CFFS P MA   A  KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLE+QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPESTS SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPS 480
           GMVLANTAANGEELVADSHLLPA+AV Q  GDIIRKYLIS+PKPTVTILFEGTKLGI+PS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQD ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSF 660
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCDSGKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGG---GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANE 720
           AVVFDGVLGG   GSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT+ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           KKSYTVTFTT TGS+APPSAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Lcy04g015170 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 547/759 (72.07%), Postives = 636/759 (83.79%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 60
           ++   + LLL   F   S ++ D+ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE+
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65

Query: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYP 120
           +Y Y N +HGFSTRLT EEA  L +QPG+++V+PE RYELHTTRTP FLGLD++ ADL+P
Sbjct: 66  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125

Query: 121 ESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARY 180
           E+ S S+V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGAR+
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           F++GYE+T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RA
Sbjct: 186 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 245

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKG 300
           RVA YKVCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+G
Sbjct: 246 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 305

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ LP
Sbjct: 306 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 365

Query: 361 GTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
             LLPFIYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM
Sbjct: 366 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 425

Query: 421 VLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPV 480
           +LANTAANGEELVAD+HLLPAT V +  GDIIR Y+ ++P PT +I   GT +G++PSPV
Sbjct: 426 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 485

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSC
Sbjct: 486 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 545

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVD 600
           PHVSGLAAL+K  HP+WSPAAIRSALMTTAY  YK+G+ + D ATGKPSTPFDHGAGHV 
Sbjct: 546 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVS 605

Query: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAV 660
           P +A NPGL+YDLT +DYL FLCALNYTS QI S++RR++TCD  K YSV DLNYPSFAV
Sbjct: 606 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

Query: 661 VFDGVLGGGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTEANEKKSYT 720
             DGV        K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F EANEKKSYT
Sbjct: 666 NVDGV-----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 725

Query: 721 VTFTTATGSAAPPSAEGFGRIEWSDGKHVVGSPIAFSWT 759
           VTFT    S+ P  +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 726 VTFT--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Lcy04g015170 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 811.6 bits (2095), Expect = 5.3e-235
Identity = 415/737 (56.31%), Postives = 533/737 (72.32%), Query Frame = 0

Query: 24  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNNVVHGFSTRLTAEEAQR- 83
           KKTYI+ +     PESF  H  WY S L S S    ++Y Y    HGFS  L + EA   
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 84  LESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVSEVIIGVLDTGVWPESKS 143
           L S   IL +  +  Y LHTTRTP+FLGL+    ++   +S + VIIGVLDTGVWPES+S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 144 FDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE-ATLGPIDESKESRSPR 203
           FDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 204 DDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFSSDILAAI 263
           D DGHGTHT+TTAAGS V NAS  GYA+GTARGMA RARVA YKVCW+ GCF SDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 264 EKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPSPYSLSNTSP 323
           ++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SCSAGN+GP+  S++N +P
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 324 WITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGTLLPFIYAANASNSANGNLCM 383
           W+ TVGAGTLDRDFPA+ +LG+GK  +GVSLY G  +    L  +Y  N  NS++ NLC+
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 384 TGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPAT 443
            G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANTAA+GEELVADSHLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 444 AVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDII 503
           AV +  GD++R+Y+ S+ KPT  ++F+GT L ++PSPVVAAFSSRGPN++TP++LKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 504 APGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAI 563
            PGVNILAGWS ++GP+GL  D RR  FNI+SGTSMSCPH+SGLA L+K AHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 564 RSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFL 623
           +SALMTTAY        + D A    S P+ HG+GHVDP  AL+PGLVYD++ ++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 624 CALNYTSSQINSLARRDFTCDSGKKYSVNDLNYPSFAVVFDGVLGGGSSVVKHTRTLTNV 683
           C+L+YT   I ++ +R     S K      LNYPSF+V+F     GG  VV++TR +TNV
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-----GGKRVVRYTREVTNV 686

Query: 684 GSPGT-YKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATGSAAPPSAEGFGRI 743
           G+  + YKV+++     V ISV+P  LSF    EKK YTVTF +  G +    AE FG I
Sbjct: 687 GAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE-FGSI 746

Query: 744 EWSDGKHVVGSPIAFSW 758
            WS+ +H V SP+AFSW
Sbjct: 747 TWSNPQHEVRSPVAFSW 751

BLAST of Lcy04g015170 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 780.8 bits (2015), Expect = 1.0e-225
Identity = 397/750 (52.93%), Postives = 521/750 (69.47%), Query Frame = 0

Query: 24  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 83
           KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y Y    HG + +
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 84  LTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESTSVSEVIIGVL 143
           LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++     ++ E  +  +V++GVL
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153

Query: 144 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYEATLGPID 203
           DTG+WPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F +GYEA  G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213

Query: 204 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 263
           E  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273

Query: 264 FSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEKGILISCSAGNAGPS 323
           FSSDIL+A+++AV D V VLS+SLGGG+S Y +DS++   F AME G+ +SCSAGN GP 
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333

Query: 324 PYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 383
           P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +       P +Y   
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393

Query: 384 NASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 443
           NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453

Query: 444 ELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLGIQPSPVVAAFSSRGPN 503
           ELVADSH+LPA AV +  G +I++Y ++  K T ++   GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513

Query: 504 SITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALI 563
            ++ ++LKPD++APGVNILA W+  + PS L+ D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573

Query: 564 KGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDPVSALNPGLV 623
           K  HPDWSPAAI+SALMTTAY      + + D +   PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633

Query: 624 YDLTVDDYLNFLCALNYTSSQINSLARR-DFTCDSGKKYSVNDLNYPSFAVVFDGVLGGG 683
           YD+   +Y  FLC  + + SQ+    +  + TC      +  +LNYP+ + +F       
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693

Query: 684 SSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTEANEKKSYTVTFTTATG 743
           +  ++  RT+TNVG    +YKVS+ S  K   ++V+P++L+FT  ++K SYTVTF T   
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 753

Query: 744 SAAPPSAEGFGRIEWSDGKHVVGSPIAFSW 758
              P     FG + W    H V SP+  +W
Sbjct: 754 MKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of Lcy04g015170 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 772.3 bits (1993), Expect = 3.6e-223
Identity = 408/764 (53.40%), Postives = 517/764 (67.67%), Query Frame = 0

Query: 9   LLLCFFSRPSMAAPDKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 68
           LLLCFFS  S ++   ++YIVH+ +   P  F  H +W+ S L+S+  +   A ++Y+Y+
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74

Query: 69  NVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESTSVS 128
             VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  S    
Sbjct: 75  RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134

Query: 129 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARYFSKGYE 188
           +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY 
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194

Query: 189 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 248
                  +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254

Query: 249 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVASGAFAAMEKGIL 308
           YK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314

Query: 309 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLPGT 368
           +SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374

Query: 369 LLPFIYAANASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 428
            L  +Y    S      LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434

Query: 429 ANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPKPTVTILFEGTKLG-IQPSPVV 488
           ANTA +GEEL ADSHL+PAT V    GD IR Y+ +   PT  I F GT +G   PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494

Query: 489 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 548
           AAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554

Query: 549 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKPSTPFDHGAGHVDP 608
           HVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ I+D ATGK S  F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614

Query: 609 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDSGKKYSVNDLNYPSF 668
             ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C++ K  +  DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674

Query: 669 AVVFDGVLGGGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTEANEK 728
           +VVF         VVK+ R + NVGS     Y+V + S    V+I V P  L+F++    
Sbjct: 675 SVVF----ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734

Query: 729 KSYTVTFTTATGSAAPPSAEG--FGRIEWSDGKHVVGSPIAFSW 758
             Y VTF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lcy04g015170 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 756.5 bits (1952), Expect = 2.0e-218
Identity = 399/770 (51.82%), Postives = 522/770 (67.79%), Query Frame = 0

Query: 1   MAKPLWILLLLCFFSRPSMAAPDKK-TYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTA 60
           MA   +   LL   S  S A+     TYIVH+     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGL--DKNAD 120
            +I+ Y+ V HGFS RLT+++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 121 LYPESTSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
           L  ES   S+++IGV+DTGVWPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 181 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
           AR+F  GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAM 300
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG +  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 360
           ++GI +S SAGN GP   +++N +PW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 361 PL-PGTLLPFIYAAN--ASNSANGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
            L PG + P +Y  +    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 421 AGGIGMVLANTAANGEELVADSHLLPATAVDQNFGDIIRKYLISEPK------PTVTILF 480
            GG+GM++AN   +GE LVAD H+LPAT+V  + GD IR+Y+    K      PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 481 EGTKLGIQPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
           +GT+LGI+P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDTATGKP 600
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 601 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDSGKKY 660
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 661 S-VNDLNYPSFAVVFDGVLGGGSSVVKH-TRTLTNVG-SPGTYKVSISSETKLVKISVEP 720
             V +LNYPSF+VVF     G S +  H  RT+TNVG S   Y++ I    +   ++VEP
Sbjct: 661 GHVGNLNYPSFSVVFQQY--GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEP 720

Query: 721 ESLSFTEANEKKSYTVTFTTATGSAAPPSAE-GFGRIEWSDGKHVVGSPI 754
           E LSF    +K S+ V   T     +P +     G I WSDGK  V SP+
Sbjct: 721 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O653510.0e+0072.07Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF67.5e-23456.31Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9FLI41.4e-22452.93Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Q9LVJ15.0e-22253.40Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
Q9LUM32.8e-21751.82Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1GEZ10.0e+0093.72subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE... [more]
A0A6J1IIG30.0e+0093.72subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3... [more]
A0A5D3CR080.0e+0093.57Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B0Y20.0e+0093.57subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=... [more]
A0A0A0KIG40.0e+0092.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038881674.10.0e+0095.93subtilisin-like protease SBT1.7 [Benincasa hispida][more]
KAG6604148.10.0e+0093.98Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023543760.10.0e+0093.84subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo][more]
KAG7034309.10.0e+0093.85Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022950492.10.0e+0093.72subtilisin-like protease SBT1.7 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G67360.10.0e+0072.07Subtilase family protein [more]
AT2G05920.15.3e-23556.31Subtilase family protein [more]
AT5G51750.11.0e-22552.93subtilase 1.3 [more]
AT3G14067.13.6e-22353.40Subtilase family protein [more]
AT3G14240.12.0e-21851.82Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 533..549
score: 58.0
coord: 124..143
score: 28.43
coord: 202..215
score: 50.52
IPR003137PA domainPFAMPF02225PAcoord: 380..450
e-value: 8.3E-10
score: 38.6
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 127..617
e-value: 1.2E-185
score: 619.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 100..608
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 15..101
e-value: 1.3E-18
score: 69.2
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 26..101
e-value: 5.3E-14
score: 52.6
NoneNo IPR availableGENE3D2.60.40.2310coord: 621..757
e-value: 5.0E-38
score: 131.8
NoneNo IPR availableGENE3D3.50.30.30coord: 332..473
e-value: 1.2E-185
score: 619.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..208
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..213
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 16..756
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 97..604
score: 29.165529
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 338..465
e-value: 1.84134E-40
score: 142.553
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 125..582
e-value: 1.8E-52
score: 178.9
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 651..754
e-value: 6.8E-25
score: 87.2
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 16..756
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 534..544
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 98..570
e-value: 1.22524E-149
score: 437.03

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g015170.1Lcy04g015170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity