Lcy04g014270 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g014270
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionsalicylic acid-binding protein 2-like
LocationChr04: 46510090 .. 46511699 (-)
RNA-Seq ExpressionLcy04g014270
SyntenyLcy04g014270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATGGCAATAATTATACAAGACAAAATGGATTTTCTGGAAATTTTTGTCGTTACAAAATCAATTATTTACATATCACTCAATGAAATTTCACTCTCAATCCCTCTCTCTCTTCATCTCTTAAACCTCATTTCTGATTAAATTAATCTAGACTTATATTCAAATGCATGAATTACATTTAATTACATACTTTGAATCTCTTTTTCTGAACAATCTCTTCTCTTCTCTCTCGATTTGTGTTGTGAGAGTCTACTACGTTACCAATAGTCCTTAACTGAAAGGAAAAAGAGGCCTGTAATCACATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGCAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGGTACGTGTATGTTTGTATGCAAATATTCTCCTTTCGTAAATTAAAGTAAAGTATAATAAGAAGGGTTTTTTTAACATTTTTCATGGTGTTTGTTGATATGAAAGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCCCTCCTGAGGTTCCCTCTATATATTTCTGATCTGTCTTTTTTTTTTTTTTTGTTGTTCTTTCGAAGGGATTTTAATTTATTTTTTTTTTAACCACAATGATTAATGAAAATTAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGTCTTCTTCAGGTTGCTCGCAACTACACTTAAATATGAGCTCCCCTGTCCATTGGCCTTGTAATAATAAAAGCATTTGAATCTCTCACCACAACTGTTTACGTGTTAATTCGAGGTAGCTCACGACTACACTTAGATATGAGCTGCCCTCGTAATAATAGAAGTCCAAGACTGCATTTGAATCTCTAACCACTATTTACGTGTTTGATGGGATTTTCTACCAAAAATGTGTTTAATTGTATATTTGAAAAATTGGATGTGTTGTCTAATATATGTAGGTTGCTATTTGAGGTAGGTTGTAAAAAGGGAATCAATGATTGTGTATGTGTATGTGTATAAGTCCATGAAAG

mRNA sequence

ATATGGCAATAATTATACAAGACAAAATGGATTTTCTGGAAATTTTTGTCGTTACAAAATCAATTATTTACATATCACTCAATGAAATTTCACTCTCAATCCCTCTCTCTCTTCATCTCTTAAACCTCATTTCTGATTAAATTAATCTAGACTTATATTCAAATGCATGAATTACATTTAATTACATACTTTGAATCTCTTTTTCTGAACAATCTCTTCTCTTCTCTCTCGATTTGTGTTGTGAGAGTCTACTACGTTACCAATAGTCCTTAACTGAAAGGAAAAAGAGGCCTGTAATCACATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGCAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCCCTCCTGAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGTCTTCTTCAGGTTGCTCGCAACTACACTTAAATATGAGCTCCCCTGTCCATTGGCCTTGTAATAATAAAAGCATTTGAATCTCTCACCACAACTGTTTACGTGTTAATTCGAGGTAGCTCACGACTACACTTAGATATGAGCTGCCCTCGTAATAATAGAAGTCCAAGACTGCATTTGAATCTCTAACCACTATTTACGTGTTTGATGGGATTTTCTACCAAAAATGTGTTTAATTGTATATTTGAAAAATTGGATGTGTTGTCTAATATATGTAGGTTGCTATTTGAGGTAGGTTGTAAAAAGGGAATCAATGATTGTGTATGTGTATGTGTATAAGTCCATGAAAG

Coding sequence (CDS)

ATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGCAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCCCTCCTGAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGTCTTCTTCAGGTTGCTCGCAACTACACTTAA

Protein sequence

MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPLLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAMFSKPLQLFQCLLQVARNYT
Homology
BLAST of Lcy04g014270 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-76
Identity = 136/258 (52.71%), Postives = 187/258 (72.48%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           E KHFVLVHGA HG W+WY++KPLLEAA H+VT LD+  +G + + I+E++++ +Y  PL
Sbjct: 3   EGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPL 62

Query: 62  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           +E M S+  +EKVILVGHS GG++L +AMEK+P+KI A+VFL AF PD+ H  S+V++++
Sbjct: 63  MELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQY 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 181
            E  P E W D+QF  +    + P TS  FGPK LA  +YQL  PED+ALA +LVR +SL
Sbjct: 123 NERTPAENWLDTQFLPY-GSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSL 182

Query: 182 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 241
           F E+L KA+ FT++ +GSVK+VY+VC EDK I  E Q+W I N G+   +EI+GADHMAM
Sbjct: 183 FMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAM 242

Query: 242 FSKPLQLFQCLLQVARNY 260
             +P +L   LL++A  Y
Sbjct: 243 LCEPQKLCASLLEIAHKY 259

BLAST of Lcy04g014270 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.1e-71
Identity = 126/258 (48.84%), Postives = 182/258 (70.54%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHG+ HGAW WY++KPLLEA  HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPL 66

Query: 63  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
            + + S+  +EKV+LVGHSFGG++LA+AMEKFPEKIS +VFLTAF PDT+H PS+V+D+F
Sbjct: 67  TKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
             ++P+E W  ++F  + +  D    S  F P  +   +YQLSP ED+ L   L+R  SL
Sbjct: 127 GSNMPQEAWMGTEFEPYGS--DNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  +L K +NF+++ YGSV +V++VC EDK I  E Q+W+I N+ +  VME+E  DHM M
Sbjct: 187 FINDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVARNY 260
           F KP QL    L++A  +
Sbjct: 247 FCKPQQLSDYFLKIADKF 262

BLAST of Lcy04g014270 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.5e-69
Identity = 129/258 (50.00%), Postives = 178/258 (68.99%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHGA HGAW WY++KPLLEA  HRVT LD+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPL 66

Query: 63  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHSFGG+SLA+AM+KFP+KIS SVF+TAF PDT+H PS+V ++F
Sbjct: 67  MQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
             S+  E W  S+  ++ +  D    S  F    +   +YQLSP ED+ L   L R +SL
Sbjct: 127 ASSMTPEGWMGSELETYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  EL K ENF+EK YGSV + Y+VC ED +I+ + Q+W+I NY    V+E+E  DHM M
Sbjct: 187 FINELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVARNY 260
           F KP  L   LL +A N+
Sbjct: 247 FCKPQLLSDHLLAIADNF 262

BLAST of Lcy04g014270 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.4e-69
Identity = 121/255 (47.45%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           +QKHFVLVHG   GAW WY++KPLLE+A H+VT +D+  AGIN + + E+ +  +Y+EPL
Sbjct: 9   QQKHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPL 68

Query: 62  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           +E MAS+ P+EKV+L+GHSFGG+SL +AME +PEKIS +VF++A  PD  H  +Y  +++
Sbjct: 69  MEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKY 128

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 181
            E  P ++  DSQF ++ N  + P  S   GP+ +A  ++Q    ED+ LAK L R  SL
Sbjct: 129 NEKCPADMMLDSQFSTYGN-PENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSL 188

Query: 182 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 241
           F ++L KA+ F+ + YGSVK+ Y+ CNEDK    E QKW +++ G + V EI+ ADHM M
Sbjct: 189 FFQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGM 248

Query: 242 FSKPLQLFQCLLQVA 257
            S+P ++ +CLL ++
Sbjct: 249 LSQPREVCKCLLDIS 262

BLAST of Lcy04g014270 vs. ExPASy Swiss-Prot
Match: Q9LFT6 (Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.9e-68
Identity = 131/260 (50.38%), Postives = 181/260 (69.62%), Query Frame = 0

Query: 1   MEQK-HFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAE 60
           ME+K HFVLVH A HGAW WY++KPLLE+A HRVT +++  +GI+ + IQ V++V EY++
Sbjct: 1   MERKHHFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSK 60

Query: 61  PLLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVD 120
           PL+ET+ S+  NE+VILVG SFGGI++A+A + FP KI   VFL AF PDT H PS+V+D
Sbjct: 61  PLIETLKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLD 120

Query: 121 RFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQA 180
           +++E +P  +  D +F SHE R +G  +    GPK +   +YQ  P ED  LAK L RQ 
Sbjct: 121 KYME-MPGGL-GDCEFSSHETR-NGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQG 180

Query: 181 SLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHM 240
           S F E+L K E F+E+ YGSV++VYV+ +EDK I  +  +W+I N+ +  V EI+G DHM
Sbjct: 181 SFFTEDLSKKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHM 240

Query: 241 AMFSKPLQLFQCLLQVARNY 260
            M SKP +LF  L  +A +Y
Sbjct: 241 VMLSKPQKLFDSLSAIATDY 257

BLAST of Lcy04g014270 vs. ExPASy TrEMBL
Match: A0A0A0KG72 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490080 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 5.4e-102
Identity = 185/262 (70.61%), Postives = 221/262 (84.35%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           + KHFVLVHGA HGAW WY+IKPLLEAA HRVT+LDM GAG+NR+AIQEV+S  EY+EPL
Sbjct: 3   QMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRRAIQEVESFEEYSEPL 62

Query: 62  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           L+TMA +GPNEKVILVGHSFGG+SLA+AME FP KISASVF+TAF PDT H PSYV+++F
Sbjct: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLEQF 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTS-FSFGPKCLADSVYQLSPPEDVALAKALVRQAS 181
           +ES+P+E W D++F   ENR DG S+S F FGPKC+A+ +YQ SP ED AL  +LVR A 
Sbjct: 123 LESLPREFWMDTEF--GENREDGGSSSWFLFGPKCMANKIYQFSPTEDQALGSSLVRPAK 182

Query: 182 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQN--YGIENVMEIEGADH 241
           LF E LGKAE FTE+NYGSVKKVYV+C ED+ I+ +LQKW+IQN   GI+NVMEI+ ADH
Sbjct: 183 LFIENLGKAEKFTEENYGSVKKVYVICGEDRTISKQLQKWMIQNSGKGIQNVMEIDEADH 242

Query: 242 MAMFSKPLQLFQCLLQVARNYT 261
           MAMFSKPLQ+ QCLLQVA++YT
Sbjct: 243 MAMFSKPLQVLQCLLQVAQSYT 262

BLAST of Lcy04g014270 vs. ExPASy TrEMBL
Match: A0A6J1IQY1 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477637 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.7e-101
Identity = 182/258 (70.54%), Postives = 218/258 (84.50%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFVLVHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 46  MEQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 105

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ ++ VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 106 LLQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 165

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           F ES+PKE W D+Q  ++    D PS+ F FGPKC A+++YQLSP ED+ALA  L+R  S
Sbjct: 166 FRESLPKEFWQDTQVSTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 225

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTEKNYGSVKKVYV+C+EDK I+ + QK +I+++GI++VMEI+GADHM 
Sbjct: 226 LFKEDLANAEKFTEKNYGSVKKVYVICSEDKTISKQFQKRMIESHGIQDVMEIDGADHMP 285

Query: 241 MFSKPLQLFQCLLQVARN 259
           MFSKPLQLF+CLLQVA N
Sbjct: 286 MFSKPLQLFKCLLQVAHN 299

BLAST of Lcy04g014270 vs. ExPASy TrEMBL
Match: A0A6J1GDS1 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453076 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.9e-100
Identity = 181/260 (69.62%), Postives = 217/260 (83.46%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRV VLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 23  MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEP 82

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M+ VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 83  LLQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 142

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLSP ED+ALA  L+R  S
Sbjct: 143 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 202

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 203 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 262

Query: 241 MFSKPLQLFQCLLQVARNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 263 MFSKPLQLFKCLLKVAHNCT 278

BLAST of Lcy04g014270 vs. ExPASy TrEMBL
Match: A0A6J1HDY0 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463111 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 8.6e-92
Identity = 166/256 (64.84%), Postives = 202/256 (78.91%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV VHGA HGAW+W++IKPLLEAA HRVT+LDM  +GI+RK IQ+V S+V+Y+EP
Sbjct: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ MA + PN+KVILVGHS GG +LAVAME+FP+KI+ASVF+ AF PDT HKPSYV+D+
Sbjct: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           + E  P E W D+QF  + N    PSTS  FGP  +A  +YQLSPPED+AL  +LVR +S
Sbjct: 147 YNEQTPMEAWLDTQFSPYGNEAK-PSTSMFFGPDFVAKKLYQLSPPEDLALGLSLVRPSS 206

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF ++L KA NF+++NYGSVKKVY+VC ED  I  E QKW+I N GIENVMEI G+DHM 
Sbjct: 207 LFMQDLSKANNFSKQNYGSVKKVYIVCTEDIGIPEEFQKWMIHNGGIENVMEINGSDHMP 266

Query: 241 MFSKPLQLFQCLLQVA 257
           MF KP QL  CLLQVA
Sbjct: 267 MFCKPHQLLHCLLQVA 281

BLAST of Lcy04g014270 vs. ExPASy TrEMBL
Match: A0A6J1KUH6 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497310 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.1e-91
Identity = 164/256 (64.06%), Postives = 202/256 (78.91%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV VHGA HGAW+W++IKPLLEAA HRVT+LDM  +GI+RK IQ+V S+V+Y+EP
Sbjct: 1   MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ MA + PN+KVILVGHS GG +LAVAME+FP+KI+ASVF+ AF PDT HKPSYV+D+
Sbjct: 61  LLKAMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTNHKPSYVLDQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           + E  P E W D+QF  +    + PSTS  FGP  +A  +YQLSPPED+AL  +L+R +S
Sbjct: 121 YNEQTPLEAWLDTQFSPYGTEAE-PSTSMFFGPNFVAKKLYQLSPPEDLALGLSLLRPSS 180

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF ++L KA NF+++ YGSVKKVY+VC EDK I  E QKW+I N GIENVMEI G+DHM 
Sbjct: 181 LFMQDLSKANNFSKQKYGSVKKVYIVCTEDKGIPEEFQKWMIHNVGIENVMEINGSDHMP 240

Query: 241 MFSKPLQLFQCLLQVA 257
           MF KP QL  CLLQVA
Sbjct: 241 MFCKPRQLLHCLLQVA 255

BLAST of Lcy04g014270 vs. NCBI nr
Match: XP_038881260.1 (salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 402.9 bits (1034), Expect = 2.1e-108
Identity = 193/262 (73.66%), Postives = 222/262 (84.73%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQ+HFVLVHGA HGAW WY+I+PLLEAA HRVTVLDM GAG++RKAIQEVKS+ EY+EP
Sbjct: 1   MEQRHFVLVHGACHGAWCWYKIQPLLEAAGHRVTVLDMAGAGVHRKAIQEVKSLEEYSEP 60

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+TMA VGPNEKVILVGHSFGG+SLA+AMEKFP  ISASVFLTA APDT H PSYV ++
Sbjct: 61  LLKTMACVGPNEKVILVGHSFGGMSLALAMEKFPHNISASVFLTALAPDTHHHPSYVSEQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTS--FSFGPKCLADSVYQLSPPEDVALAKALVRQ 180
           F+ES+PKE W D+Q C +ENRVD  ++S  F FGPKC+A+ +Y LSP ED+AL K+LVR 
Sbjct: 121 FLESLPKEFWMDTQSCGYENRVDDEASSSYFLFGPKCMANFIYHLSPSEDLALGKSLVRP 180

Query: 181 ASLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADH 240
            SL  EEL KAE FTE+NYGSVKKVYV+C EDK I    QKW+IQNYGI+NVMEIEGADH
Sbjct: 181 GSLLVEELAKAEKFTEENYGSVKKVYVICGEDKTIPKHFQKWMIQNYGIQNVMEIEGADH 240

Query: 241 MAMFSKPLQLFQCLLQVARNYT 261
           M MFSKPLQ+ QCLLQVA N+T
Sbjct: 241 MPMFSKPLQVLQCLLQVAHNFT 262

BLAST of Lcy04g014270 vs. NCBI nr
Match: XP_011657843.2 (salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical protein Csa_003158 [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 5.0e-102
Identity = 186/262 (70.99%), Postives = 221/262 (84.35%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           + KHFVLVHGA HGAW WY+IKPLLEAA HRVT+LDM GAG+NRKAIQEV+S  EY+EPL
Sbjct: 3   QMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRKAIQEVESFEEYSEPL 62

Query: 62  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           L+TMA +GPNEKVILVGHSFGG+SLA+AME FP KISASVF+TAF PDT H PSYV+++F
Sbjct: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLEQF 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTS-FSFGPKCLADSVYQLSPPEDVALAKALVRQAS 181
           +ES+P+E W D++F   ENR DG S+S F FGPKC+A+ +YQ SP ED AL  +LVR A 
Sbjct: 123 LESLPREFWMDTEF--GENREDGGSSSWFLFGPKCMANKIYQFSPTEDQALGSSLVRPAK 182

Query: 182 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQN--YGIENVMEIEGADH 241
           LF E LGKAE FTE+NYGSVKKVYV+C ED+ I+ +LQKW+IQN   GI+NVMEI+ ADH
Sbjct: 183 LFIENLGKAEKFTEENYGSVKKVYVICGEDRTISKQLQKWMIQNSGKGIQNVMEIDEADH 242

Query: 242 MAMFSKPLQLFQCLLQVARNYT 261
           MAMFSKPLQ+ QCLLQVA++YT
Sbjct: 243 MAMFSKPLQVLQCLLQVAQSYT 262

BLAST of Lcy04g014270 vs. NCBI nr
Match: XP_022977269.1 (salicylic acid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 378.3 bits (970), Expect = 5.5e-101
Identity = 182/258 (70.54%), Postives = 218/258 (84.50%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFVLVHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 46  MEQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 105

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ ++ VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 106 LLQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 165

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           F ES+PKE W D+Q  ++    D PS+ F FGPKC A+++YQLSP ED+ALA  L+R  S
Sbjct: 166 FRESLPKEFWQDTQVSTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 225

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTEKNYGSVKKVYV+C+EDK I+ + QK +I+++GI++VMEI+GADHM 
Sbjct: 226 LFKEDLANAEKFTEKNYGSVKKVYVICSEDKTISKQFQKRMIESHGIQDVMEIDGADHMP 285

Query: 241 MFSKPLQLFQCLLQVARN 259
           MFSKPLQLF+CLLQVA N
Sbjct: 286 MFSKPLQLFKCLLQVAHN 299

BLAST of Lcy04g014270 vs. NCBI nr
Match: XP_022949785.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 8.0e-100
Identity = 181/260 (69.62%), Postives = 217/260 (83.46%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRV VLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 23  MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEP 82

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M+ VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 83  LLQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 142

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLSP ED+ALA  L+R  S
Sbjct: 143 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 202

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 203 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 262

Query: 241 MFSKPLQLFQCLLQVARNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 263 MFSKPLQLFKCLLKVAHNCT 278

BLAST of Lcy04g014270 vs. NCBI nr
Match: KAG6604201.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372.1 bits (954), Expect = 4.0e-99
Identity = 180/260 (69.23%), Postives = 216/260 (83.08%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 1   MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 60

Query: 61  LLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M+ V P EKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 61  LLQAMSCVDPKEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSAVIEQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLSP ED+ALA  L+R  S
Sbjct: 121 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 180

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 181 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 240

Query: 241 MFSKPLQLFQCLLQVARNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 241 MFSKPLQLFKCLLKVAHNCT 256

BLAST of Lcy04g014270 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 268.5 bits (685), Expect = 5.7e-72
Identity = 126/258 (48.84%), Postives = 182/258 (70.54%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHG+ HGAW WY++KPLLEA  HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPL 66

Query: 63  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
            + + S+  +EKV+LVGHSFGG++LA+AMEKFPEKIS +VFLTAF PDT+H PS+V+D+F
Sbjct: 67  TKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
             ++P+E W  ++F  + +  D    S  F P  +   +YQLSP ED+ L   L+R  SL
Sbjct: 127 GSNMPQEAWMGTEFEPYGS--DNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  +L K +NF+++ YGSV +V++VC EDK I  E Q+W+I N+ +  VME+E  DHM M
Sbjct: 187 FINDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVARNY 260
           F KP QL    L++A  +
Sbjct: 247 FCKPQQLSDYFLKIADKF 262

BLAST of Lcy04g014270 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-70
Identity = 129/258 (50.00%), Postives = 178/258 (68.99%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHGA HGAW WY++KPLLEA  HRVT LD+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPL 66

Query: 63  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHSFGG+SLA+AM+KFP+KIS SVF+TAF PDT+H PS+V ++F
Sbjct: 67  MQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
             S+  E W  S+  ++ +  D    S  F    +   +YQLSP ED+ L   L R +SL
Sbjct: 127 ASSMTPEGWMGSELETYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  EL K ENF+EK YGSV + Y+VC ED +I+ + Q+W+I NY    V+E+E  DHM M
Sbjct: 187 FINELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVARNY 260
           F KP  L   LL +A N+
Sbjct: 247 FCKPQLLSDHLLAIADNF 262

BLAST of Lcy04g014270 vs. TAIR 10
Match: AT5G10300.1 (methyl esterase 5 )

HSP 1 Score: 260.0 bits (663), Expect = 2.0e-69
Identity = 131/260 (50.38%), Postives = 181/260 (69.62%), Query Frame = 0

Query: 1   MEQK-HFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAE 60
           ME+K HFVLVH A HGAW WY++KPLLE+A HRVT +++  +GI+ + IQ V++V EY++
Sbjct: 1   MERKHHFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSK 60

Query: 61  PLLETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVD 120
           PL+ET+ S+  NE+VILVG SFGGI++A+A + FP KI   VFL AF PDT H PS+V+D
Sbjct: 61  PLIETLKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLD 120

Query: 121 RFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQA 180
           +++E +P  +  D +F SHE R +G  +    GPK +   +YQ  P ED  LAK L RQ 
Sbjct: 121 KYME-MPGGL-GDCEFSSHETR-NGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQG 180

Query: 181 SLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHM 240
           S F E+L K E F+E+ YGSV++VYV+ +EDK I  +  +W+I N+ +  V EI+G DHM
Sbjct: 181 SFFTEDLSKKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHM 240

Query: 241 AMFSKPLQLFQCLLQVARNY 260
            M SKP +LF  L  +A +Y
Sbjct: 241 VMLSKPQKLFDSLSAIATDY 257

BLAST of Lcy04g014270 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 254.2 bits (648), Expect = 1.1e-67
Identity = 121/258 (46.90%), Postives = 181/258 (70.16%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++H VLVHGA HGAW WY++KP LEA+ HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPL 66

Query: 63  LETMASVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHS GG+SLA+AM+ FP KIS SVF+TA  PDT+H PS+V D+ 
Sbjct: 67  MQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKL 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
            +   +E W D+ F S   + D PS  + FGP+ +A ++YQLSP +D+ LAK LVR   L
Sbjct: 127 RKETSREEWLDTVFTS--EKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
             +++ +  +F+E+ YGSV ++++VC +D +   + Q+ +I N+  + VMEI+ ADHM M
Sbjct: 187 IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDADHMPM 246

Query: 243 FSKPLQLFQCLLQVARNY 260
           FSKP QL   LL++A  Y
Sbjct: 247 FSKPQQLCALLLEIANKY 262

BLAST of Lcy04g014270 vs. TAIR 10
Match: AT2G23580.1 (methyl esterase 4 )

HSP 1 Score: 242.3 bits (617), Expect = 4.4e-64
Identity = 119/258 (46.12%), Postives = 174/258 (67.44%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPLL 62
           +K FVLVHG  HGAW WY++K  LEA  H VT +D+  +GIN   ++E++++ +Y +PLL
Sbjct: 6   KKRFVLVHGLCHGAWCWYKVKTHLEAVGHCVTAVDLAASGINMTRLEEIQTLKDYCKPLL 65

Query: 63  ETMASVG-PNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           E + S+G  ++KVILV HS GGI  A+A + FP KI+  VFLTAF PDT++ P+YV  + 
Sbjct: 66  ELLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFMPDTRNLPAYVYQKL 125

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSPPEDVALAKALVRQASL 182
           I SVP+E W D+ F ++  + + P     FGPK +A ++YQLSP +D+ LAK LVR   +
Sbjct: 126 IRSVPQEGWLDTVFGTY-GKHECPLEFALFGPKFMAKNLYQLSPVQDLELAKMLVRVNPI 185

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
               L    +F+E+ YG+V ++Y+VC ED  +  + Q W+I+N+  + VMEI+ ADHMAM
Sbjct: 186 ITNNLAGTRSFSEEGYGTVTRIYIVCGEDMAVPEDYQWWMIKNFPPKEVMEIKCADHMAM 245

Query: 243 FSKPLQLFQCLLQVARNY 260
           FSKP +L   L+++A  Y
Sbjct: 246 FSKPHKLCALLVEIACKY 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6RYA01.3e-7652.71Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1[more]
Q8S8S98.1e-7148.84Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1[more]
O804761.5e-6950.00Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1[more]
Q9SE934.4e-6947.45Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=... [more]
Q9LFT62.9e-6850.38Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KG725.4e-10270.61AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490... [more]
A0A6J1IQY12.7e-10170.54salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11147763... [more]
A0A6J1GDS13.9e-10069.62salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453... [more]
A0A6J1HDY08.6e-9264.84salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
A0A6J1KUH61.1e-9164.06salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149731... [more]
Match NameE-valueIdentityDescription
XP_038881260.12.1e-10873.66salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida][more]
XP_011657843.25.0e-10270.99salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical pr... [more]
XP_022977269.15.5e-10170.54salicylic acid-binding protein 2-like [Cucurbita maxima][more]
XP_022949785.18.0e-10069.62salicylic acid-binding protein 2-like [Cucurbita moschata][more]
KAG6604201.14.0e-9969.23Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT2G23620.15.7e-7248.84methyl esterase 1 [more]
AT2G23600.11.1e-7050.00acetone-cyanohydrin lyase [more]
AT5G10300.12.0e-6950.38methyl esterase 5 [more]
AT2G23610.11.1e-6746.90methyl esterase 3 [more]
AT2G23580.14.4e-6446.12methyl esterase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..260
e-value: 8.0E-74
score: 250.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 5..255
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 6..247
e-value: 2.6E-18
score: 67.4
NoneNo IPR availablePANTHERPTHR10992:SF1027METHYLESTERASE 1-RELATEDcoord: 3..259
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 3..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g014270.1Lcy04g014270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity