Lcy04g013630 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g013630
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptiontRNA(His) guanylyltransferase
LocationChr04: 45713772 .. 45715615 (+)
RNA-Seq ExpressionLcy04g013630
SyntenyLcy04g013630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATAGTGAGTATGAGTATGTGAAGTTGTTCGAAGTTGATGATGAGGTGATGCTGCCCAATCTGATCGTCGTTCGGGTTGATGGGCTCGACTTTCGTAGGTTTTCTCACTTAGCAATTCAATTTTGGCAATATTAAACTTTCAAGATATCGTGCCTCGTGATTGTGCCAAGTGTGAGAGCATTGAATTTCTGTGGCCTTTACTAATCTTCTCTAATTCATTCTTGATGCACTTTCTATGGGTTTATATCAATATCTTCAAAGCATAAGGTGTACACTAACTAAGACTATAAGCTTCTTGCTTGCCTGGAGGTGAGAGGAATATATACATCACTAGGTGAGGTGAGGCATTTAAACTGAAAATAGGAATGCATTATTACACAAGATGAAATTTATGATGGACGTGAATAAGATGATATTTGGTCTGGTGGTCAATTTAGAGTCAATATAAATAATAAAGGGTTTAGAGGAAATAGATTCAAGTCATGGTGGCCACCTACCTAAGATTTAATTTTCTATGACATTGATGTGTCCTGTATTTTCAAGTGCTGGAGAATGAAAAAAGAATAGATTGATTGAGTATATAGCGTCAGTGCTAAGGGATAAACTGCGCTATAAACATTCCATGGGACTGTCATAAGCTTAAGTAAAGGATTTTTGTATATGTGATGAGTGCCCTTTTGTTTCTTGTCCATCGCAATATTGCTCAGAATCTTTTTCCCTTCTTTGGCAGATTCTCTGAAGTTCATGGGTTTGTGAAGCCAAATGACGAAAATGTTTTAAAGTTGATGAATTATTGTGCAATCGCCACAATGGAAATGTTTTCAGATGTAGTATTTTCATATGGATTTAGTGATGAGTACAGGTGTTCAGTTAGTCAATTATTATGTCATCTCCACCCTTTTAACTATTTATTAGCAAGTTTGTTGTCTCTTTCTTACATTACATCAATTATTGATATATTCTTTGTTAACAGTTTTATCTTTAAAATGACATCCAAGTTTTACCAAAGGCGTGCCAGATTTCTCCATTGATTTATGATCCTCTGTTTTGATGTTTTTATGTCATTGTTCCCCTTGAATTCTATGTACATGTGATGTAGAAAAAATCCCTTTATTCTTTTTTGCTGAGCTGATGCATGGGGGTTGTTTTTGTCATGATATTAATATCGATACTTTTTTTTTCCTTCTTTTCTTTCTTTCTTTTCTGGGTCAGAGCCAATAATTTTGATTTTTCTAAGATTTGAAAATTAGATTTTTATCTAATTAAGTTTTAGGATTTGAGATTTATAAATAAAATGAAGAATTTTTTTTATTAGAATTTGAATGGTAGATGTTTCTTATCGTTATAATTTCTGTTATAAATTCAATGTTCCTTTCGTTTATTAGTTTTCTTCTTTCGTTGAAATTTATGTTGATGTGCTTACAAATTGGGCTTATGCAACAAATTATATTCGCTCGTTGTATGTATATATAACAAAATGGCCAGAATTCTTTCCTCAGCAGAACTTGAGCTACCCACCTTCATTTCGTGCACGTGTAGTATGTTGTGCATCTATAGAAGTTCTTCAAGCATATCTTGCATGGAGACAACAGTTCTGTAAGTGGTTGCCTTTTGGTTTGCAAATCCATAGTATTCCTAGATCTTGCAGGACCTACAATGAAGTTGTAAGTTTTGTTTGATCTTCAAATACCTTCTTTTGTTGGCTTGGCAGGCAGAATGTCCTTATTTAAACTTTCCAAATTCACCTTTTTCAGGTCATATCAGTAACTTGGACAATACATGCTTTTGGAAGCTGGTCGAGTGTGGGAAGACAGACAGGGAAGCCCATGATTTTCTGA

mRNA sequence

ATGGCAAATAGTGAGTATGAGTATGTGAAGTTGTTCGAAGTTGATGATGAGGTGATGCTGCCCAATCTGATCGTCGTTCGGAACTTGAGCTACCCACCTTCATTTCGTGCACGTGTAGTATGTTGTGCATCTATAGAAGTTCTTCAAGCATATCTTGCATGGAGACAACAGTTCTGTAAGTGGTTGCCTTTTGGTTTGCAAATCCATAGTATTCCTAGATCTTGCAGGACCTACAATGAAGTTGTCATATCAGTAACTTGGACAATACATGCTTTTGGAAGCTGGTCGAGTGTGGGAAGACAGACAGGGAAGCCCATGATTTTCTGA

Coding sequence (CDS)

ATGGCAAATAGTGAGTATGAGTATGTGAAGTTGTTCGAAGTTGATGATGAGGTGATGCTGCCCAATCTGATCGTCGTTCGGAACTTGAGCTACCCACCTTCATTTCGTGCACGTGTAGTATGTTGTGCATCTATAGAAGTTCTTCAAGCATATCTTGCATGGAGACAACAGTTCTGTAAGTGGTTGCCTTTTGGTTTGCAAATCCATAGTATTCCTAGATCTTGCAGGACCTACAATGAAGTTGTCATATCAGTAACTTGGACAATACATGCTTTTGGAAGCTGGTCGAGTGTGGGAAGACAGACAGGGAAGCCCATGATTTTCTGA

Protein sequence

MANSEYEYVKLFEVDDEVMLPNLIVVRNLSYPPSFRARVVCCASIEVLQAYLAWRQQFCKWLPFGLQIHSIPRSCRTYNEVVISVTWTIHAFGSWSSVGRQTGKPMIF
Homology
BLAST of Lcy04g013630 vs. ExPASy Swiss-Prot
Match: F4ISV6 (tRNA(His) guanylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=THG2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.5e-07
Identity = 43/151 (28.48%), Postives = 50/151 (33.11%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVK FEV+DEVM PNLI++R                                 
Sbjct: 1   MANSKYEYVKSFEVEDEVMFPNLIIIRIDGRDFSRFSQVHKFEKPNDETSLNLMNSCASS 60

BLAST of Lcy04g013630 vs. ExPASy Swiss-Prot
Match: F4IRQ5 (tRNA(His) guanylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=THG1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 5.8e-07
Identity = 45/151 (29.80%), Postives = 51/151 (33.77%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVV---------------------------------- 60
           MANS+YEYVK FE++DEVMLPNL+VV                                  
Sbjct: 48  MANSKYEYVKSFELEDEVMLPNLMVVRIDGRDFSRFSQVHEFEKPNDETALNLMNSCSAA 107

BLAST of Lcy04g013630 vs. ExPASy TrEMBL
Match: A0A0A0KFW7 (tRNA(His) guanylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G486910 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 3.1e-11
Identity = 56/151 (37.09%), Postives = 57/151 (37.75%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVMLPNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMLPNLIVVRVDGLDFRRFSEVHGFVKPNDEKALELMNCCAMA 60

BLAST of Lcy04g013630 vs. ExPASy TrEMBL
Match: A0A6J1GED7 (tRNA(His) guanylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111453416 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 3.4e-10
Identity = 54/151 (35.76%), Postives = 57/151 (37.75%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVM PNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMPPNLIVVRVEGLDFPRFSEVHGFVKPNDEKALELMNCCAMA 60

BLAST of Lcy04g013630 vs. ExPASy TrEMBL
Match: A0A6J1BSQ5 (tRNA(His) guanylyltransferase OS=Momordica charantia OX=3673 GN=LOC111005370 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.6e-10
Identity = 53/151 (35.10%), Postives = 55/151 (36.42%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVMLPNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMLPNLIVVRVYGLDFRRFSEVHGFVKPNDENAVKLMNHCAMA 60

BLAST of Lcy04g013630 vs. ExPASy TrEMBL
Match: A0A6J1IJJ9 (tRNA(His) guanylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478015 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.6e-10
Identity = 53/151 (35.10%), Postives = 57/151 (37.75%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVM PNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMPPNLIVVRVEGLDFPRFSEVHGFVKPNDEKALELMNCCAMA 60

BLAST of Lcy04g013630 vs. ExPASy TrEMBL
Match: A0A103XE31 (tRNA(His) guanylyltransferase OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_008976 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.6e-10
Identity = 41/92 (44.57%), Postives = 50/92 (54.35%), Query Frame = 0

Query: 1  MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
          MANS+YEYVK FE +DE+M PNLIVVR                                 
Sbjct: 1  MANSKYEYVKSFENEDEMMYPNLIVVRIDGRNFESKMLSLIVSFFASLYVAKWREFFPTK 60

BLAST of Lcy04g013630 vs. NCBI nr
Match: XP_038883678.1 (tRNA(His) guanylyltransferase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-13
Identity = 57/136 (41.91%), Postives = 58/136 (42.65%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVMLPNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMLPNLIVVRVDGLDFRSFSEVHGFVKPNDEKALELMNCCAMS 60

BLAST of Lcy04g013630 vs. NCBI nr
Match: XP_038883677.1 (tRNA(His) guanylyltransferase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 80.9 bits (198), Expect = 7.6e-12
Identity = 57/151 (37.75%), Postives = 58/151 (38.41%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVMLPNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMLPNLIVVRVDGLDFRSFSEVHGFVKPNDEKALELMNCCAMS 60

BLAST of Lcy04g013630 vs. NCBI nr
Match: XP_004141969.1 (tRNA(His) guanylyltransferase 1 isoform X1 [Cucumis sativus] >KGN48418.1 hypothetical protein Csa_004227 [Cucumis sativus])

HSP 1 Score: 77.8 bits (190), Expect = 6.4e-11
Identity = 56/151 (37.09%), Postives = 57/151 (37.75%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVMLPNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMLPNLIVVRVDGLDFRRFSEVHGFVKPNDEKALELMNCCAMA 60

BLAST of Lcy04g013630 vs. NCBI nr
Match: XP_039049781.1 (tRNA(His) guanylyltransferase 2-like isoform X3 [Hibiscus syriacus])

HSP 1 Score: 77.0 bits (188), Expect = 1.1e-10
Identity = 45/107 (42.06%), Postives = 52/107 (48.60%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVK FEV+DE+M PNLIV+R                                 
Sbjct: 1   MANSKYEYVKSFEVEDEIMPPNLIVIRIDGRDFRSFVLKKTSKFYQRRSSKISSVIVSCF 60

BLAST of Lcy04g013630 vs. NCBI nr
Match: KAG6604248.1 (tRNA(His) guanylyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 74.3 bits (181), Expect = 7.1e-10
Identity = 54/151 (35.76%), Postives = 57/151 (37.75%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVKLFEVDDEVM PNLIVVR                                 
Sbjct: 1   MANSKYEYVKLFEVDDEVMPPNLIVVRVEGLDFPRFSEVHGFVKPNDEKALELMNCCAIA 60

BLAST of Lcy04g013630 vs. TAIR 10
Match: AT2G32320.1 (tRNAHis guanylyltransferase )

HSP 1 Score: 55.5 bits (132), Expect = 3.2e-08
Identity = 43/151 (28.48%), Postives = 50/151 (33.11%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVK FEV+DEVM PNLI++R                                 
Sbjct: 1   MANSKYEYVKSFEVEDEVMFPNLIIIRIDGRDFSRFSQVHKFEKPNDETSLNLMNSCASS 60

BLAST of Lcy04g013630 vs. TAIR 10
Match: AT2G32320.2 (tRNAHis guanylyltransferase )

HSP 1 Score: 55.5 bits (132), Expect = 3.2e-08
Identity = 43/151 (28.48%), Postives = 50/151 (33.11%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVK FEV+DEVM PNLI++R                                 
Sbjct: 1   MANSKYEYVKSFEVEDEVMFPNLIIIRIDGRDFSRFSQVHKFEKPNDETSLNLMNSCASS 60

BLAST of Lcy04g013630 vs. TAIR 10
Match: AT2G32320.3 (tRNAHis guanylyltransferase )

HSP 1 Score: 55.5 bits (132), Expect = 3.2e-08
Identity = 43/151 (28.48%), Postives = 50/151 (33.11%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVVR--------------------------------- 60
           MANS+YEYVK FEV+DEVM PNLI++R                                 
Sbjct: 1   MANSKYEYVKSFEVEDEVMFPNLIIIRIDGRDFSRFSQVHKFEKPNDETSLNLMNSCASS 60

BLAST of Lcy04g013630 vs. TAIR 10
Match: AT2G31580.1 (tRNAHis guanylyltransferase )

HSP 1 Score: 55.1 bits (131), Expect = 4.1e-08
Identity = 45/151 (29.80%), Postives = 51/151 (33.77%), Query Frame = 0

Query: 1   MANSEYEYVKLFEVDDEVMLPNLIVV---------------------------------- 60
           MANS+YEYVK FE++DEVMLPNL+VV                                  
Sbjct: 48  MANSKYEYVKSFELEDEVMLPNLMVVRIDGRDFSRFSQVHEFEKPNDETALNLMNSCSAA 107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4ISV64.5e-0728.48tRNA(His) guanylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=THG2 PE=1 SV=... [more]
F4IRQ55.8e-0729.80tRNA(His) guanylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=THG1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KFW73.1e-1137.09tRNA(His) guanylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G486910 PE=3 SV... [more]
A0A6J1GED73.4e-1035.76tRNA(His) guanylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111453416 PE=3... [more]
A0A6J1BSQ57.6e-1035.10tRNA(His) guanylyltransferase OS=Momordica charantia OX=3673 GN=LOC111005370 PE=... [more]
A0A6J1IJJ97.6e-1035.10tRNA(His) guanylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478015 PE=3 S... [more]
A0A103XE317.6e-1044.57tRNA(His) guanylyltransferase OS=Cynara cardunculus var. scolymus OX=59895 GN=Cc... [more]
Match NameE-valueIdentityDescription
XP_038883678.11.4e-1341.91tRNA(His) guanylyltransferase 1-like isoform X2 [Benincasa hispida][more]
XP_038883677.17.6e-1237.75tRNA(His) guanylyltransferase 1-like isoform X1 [Benincasa hispida][more]
XP_004141969.16.4e-1137.09tRNA(His) guanylyltransferase 1 isoform X1 [Cucumis sativus] >KGN48418.1 hypothe... [more]
XP_039049781.11.1e-1042.06tRNA(His) guanylyltransferase 2-like isoform X3 [Hibiscus syriacus][more]
KAG6604248.17.1e-1035.76tRNA(His) guanylyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G32320.13.2e-0828.48tRNAHis guanylyltransferase [more]
AT2G32320.23.2e-0828.48tRNAHis guanylyltransferase [more]
AT2G32320.33.2e-0828.48tRNAHis guanylyltransferase [more]
AT2G31580.14.1e-0829.80tRNAHis guanylyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038469tRNAHis guanylyltransferase Thg1 superfamilyGENE3D3.30.70.3000coord: 27..91
e-value: 2.0E-6
score: 29.2
IPR007537tRNAHis guanylyltransferase Thg1PANTHERPTHR12729UNCHARACTERIZEDcoord: 1..27
IPR007537tRNAHis guanylyltransferase Thg1PANTHERPTHR12729UNCHARACTERIZEDcoord: 28..60
NoneNo IPR availablePANTHERPTHR12729:SF6TRNA(HIS) GUANYLYLTRANSFERASE-RELATEDcoord: 1..27
NoneNo IPR availablePANTHERPTHR12729:SF6TRNA(HIS) GUANYLYLTRANSFERASE-RELATEDcoord: 28..60

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g013630.1Lcy04g013630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008193 tRNA guanylyltransferase activity