Lcy04g011300 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g011300
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectate lyase
LocationChr04: 42852571 .. 42855943 (+)
RNA-Seq ExpressionLcy04g011300
SyntenyLcy04g011300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGGCCACAGCCTGCATTGTGCTTCTAGCTTTGTTGCTACCCTCTGTCCCGCTGCACGGTGCCGCTGCCGCACTCAATCACACTCTCCCCGGCCAGCATCCGAGCCCGGAACTGGTTGCACAAGAAGTTCACAGGTCCATCAATTTTCCATTTCAGATTCACAAATGTTGTTTTAAAATCACTATGTTCTTGGCTTGGCTCTCACTCTAACTGTCTGCATTTTGAAACTTTTAGCTACATTGTCAATTTTCTGTACTTTATTGGCCCATTTCAAGATTTTCATGCATCTTTTTGTAGGGTTTTATGTATTAGATGTACAAAAATCAAGTTTGAAACCAGGTCTTTTTGTTACTATGTTCATGTCTTGGCTGCTCATTTTTCTTGACCTTGTTTAATATTAGCCCTTTGTGTATCTACATTCTTCAGTTTTAAGTTTTTCTTGACCACATTATTAGGTCATTAAAATCTAAAACTTATGCTTAAATCTTACTCCTCATCTCTTCTTCTGTTTAGTAACTATTATGTTTCTGACCCATTATATGAACTTTAGCAGCATTCTCGGATTTCTTCAAATATTTAATCATTTCTGAAATCTTGACCTACTTCTTTTACAGATTTCCCACTGTTTCTGACCCTACTGTTTAGTAATTATAAGAAAAAGAACAATGCCGTTCAAATCTACTGTTTGAACGGCTCATCCTCCTTTGTTTATGGATCTTTGCACTTTGGAAATCCCATTTCCACCGTCAGTTTTTCTTTTCATTTCCTATCCTCATCTTCATGCACTGTTCTTTGTTAGATTTAGACATTTTTTGAGAGGAAAACACTGTATAATCCTTCTATTTCACTACAGTTCTTGGCTACTCTGTTTTCTGGTCAGCACTAAAAATCCCTATGTTTCTACTACCTGCATTCTTCGTCTGTCGTTCGTTCAGATCATTTTTCACCTTTGAAGGTATATTTTGTCAGATTCAACCATTTGGGGGCTGAAATTTAGCTTAATACCATCTAGGTTTAACACTAAGTTCCTCGAATCTCTAAAAATTATGAAACGAAATTGTTACCTATTGCAGGAAAGTGAATGCTTCCATAACGAGAAGGCAATTGCTCCAACTCTCAGAGAAAGACGAATCTTCTTCCTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAATTGGCCAAACAACCGCCAACGCCTCGCCGATTGCGCCATAGGATTTGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTGACAGACGACTCCGATGACGATCCGGTCAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTCTGGATCGTCTTCCCCGCCAACATGCTCATCAAACTCTCACAGGAGCTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAACGTCCACATCGTCGGAGGCGGCTGCATCACCTTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGCTATCCATCAGGTAACGAAATCGAAAGCCCTAAATTACCTTGCTGCACACCAAATGTTCGACGAAATGCATCGATCTGAAGCTCGTCTCTGGCCGTGGCTCTGCAGGTAACACGATGGTCCGATCGAGTCCGACGCATTACGGTTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGGTCGAAGGACATATGGATTGACCATTGTTCGCTGTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACGATTTCAAACAATTATTTCTCGCATCACGATGAGGTTATGCTGCTGGGTCACAGTGACACCTACTTGCCGGACTCCGGAATGCAGGTCAATTTCTCATCCTTTTTCATTCTTTCTCTCCATATATTAATTTTTTATTCTCTCCACTTGAATAAATAATTAATCGTTGTCCTGTTTTCAAAATAACAATAATAATTAATCTCTTCTTAAAAAAAAAAAAAATAATAAAATAACAATAATAATTAATCATTCTTTTCAAATAATAATAATAATAATTAATCCTTATCCTAATTCCTACCTACCTTTATATTTTTATAAAATATAAAATAAATTATTTAGAGTATCTTAATAGTTAATTAAGATCTCCTCAAAATAATAATAATAATAATTGTTAACCTAAGATACTTATTCAATGAGATAGTAAATAAATTATTTAAAGTTAATTCTTATCATCAATTTTTTGAGATAGTAATCAATGTTTTATTAAGTTATGCCTATAAATTTAGACCTCATTTGATAATCATTTAAGACTTTGTTTGGCCATGAGCATAGTTCAACTGGCATAAGTCTTGTACTAACGACCAAAAGGTTTGTGGTTTGAATCCCCCGTGTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGTTTGACAGCCATTTTGTTTTGCGTTTTTTAAAATTAAGTTTGTTTTCTCTCAATTTTCTAAGAATGGTTCACAAGTTTATTAAGGACACATATGCATTTCTAGCCAAATTCAAAAACTAACTTTTAAAAGTTTATTAAGGACACATTTAAGTACTATTAATTTTGTGTCAAATAAACTATTCGATTTAAAATTGTCTAGGTTTGGATGCTTTTTAAAGTTTATAAAGCTATTAATCATAATAAATAAAACTAGGCAAAAACTTTTTTTTATTTCTATTGGTCGAATTTTGTCGTAAACGAAGAAGAACGTGAGAATGACGATTGAATGACTGAATGAATGAAAGGTAACCATAGCATTCAACCACTTCGGGGAGAAGCTAGTGCAAAGGATGCCGCGATGCCGACGTGGCTACATCCACGTGGTCAACAACGACTTCACCCAATGGGAGATGTACGCAATCGGCGGCAGCGGAAACCCCACAATCAACAGCCAAGGCAACCGCTACACCGCGCCATACGACCGCAACGCCAAGGAGGTAACCAAGCGGGTCGAGACCGCCGAGTCCGAGTGGCGCGGCTGGAACTGGCGCTCCGACGGCGACATCCTCGTCAACGGCGCCTTCTTTGTCACTTCCGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAGTCCGCCGCGCTGATCGACCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGACAACAATTTGGGGATGTGGACCACTGGCCCTAATGGCGGAGGCTTTGCGATTGGGTCTGGCCCGGATTACACTGACGACATGTCGGAAAGCTGGTCGAGGTCTCGTTATTTTGTTATGGCGTTCTCGCTTTTGTTTTGTTCTTTTTGTGCATTGGCACTTGTCTTTGTTAGTTCTTAG

mRNA sequence

ATGCTGGCCACAGCCTGCATTGTGCTTCTAGCTTTGTTGCTACCCTCTGTCCCGCTGCACGGTGCCGCTGCCGCACTCAATCACACTCTCCCCGGCCAGCATCCGAGCCCGGAACTGGTTGCACAAGAAGTTCACAGGAAAGTGAATGCTTCCATAACGAGAAGGCAATTGCTCCAACTCTCAGAGAAAGACGAATCTTCTTCCTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAATTGGCCAAACAACCGCCAACGCCTCGCCGATTGCGCCATAGGATTTGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTGACAGACGACTCCGATGACGATCCGGTCAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTCTGGATCGTCTTCCCCGCCAACATGCTCATCAAACTCTCACAGGAGCTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAACGTCCACATCGTCGGAGGCGGCTGCATCACCTTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGCTATCCATCAGGTAACACGATGGTCCGATCGAGTCCGACGCATTACGGTTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGGTCGAAGGACATATGGATTGACCATTGTTCGCTGTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACGATTTCAAACAATTATTTCTCGCATCACGATGAGGTTATGCTGCTGGGTCACAGTGACACCTACTTGCCGGACTCCGGAATGCAGGTAACCATAGCATTCAACCACTTCGGGGAGAAGCTAGTGCAAAGGATGCCGCGATGCCGACGTGGCTACATCCACGTGGTCAACAACGACTTCACCCAATGGGAGATGTACGCAATCGGCGGCAGCGGAAACCCCACAATCAACAGCCAAGGCAACCGCTACACCGCGCCATACGACCGCAACGCCAAGGAGGTAACCAAGCGGGTCGAGACCGCCGAGTCCGAGTGGCGCGGCTGGAACTGGCGCTCCGACGGCGACATCCTCGTCAACGGCGCCTTCTTTGTCACTTCCGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAGTCCGCCGCGCTGATCGACCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGACAACAATTTGGGGATGTGGACCACTGGCCCTAATGGCGGAGGCTTTGCGATTGGGTCTGGCCCGGATTACACTGACGACATGTCGGAAAGCTGGTCGAGGTCTCGTTATTTTGTTATGGCGTTCTCGCTTTTGTTTTGTTCTTTTTGTGCATTGGCACTTGTCTTTGTTAGTTCTTAG

Coding sequence (CDS)

ATGCTGGCCACAGCCTGCATTGTGCTTCTAGCTTTGTTGCTACCCTCTGTCCCGCTGCACGGTGCCGCTGCCGCACTCAATCACACTCTCCCCGGCCAGCATCCGAGCCCGGAACTGGTTGCACAAGAAGTTCACAGGAAAGTGAATGCTTCCATAACGAGAAGGCAATTGCTCCAACTCTCAGAGAAAGACGAATCTTCTTCCTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAATTGGCCAAACAACCGCCAACGCCTCGCCGATTGCGCCATAGGATTTGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTGACAGACGACTCCGATGACGATCCGGTCAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTCTGGATCGTCTTCCCCGCCAACATGCTCATCAAACTCTCACAGGAGCTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAACGTCCACATCGTCGGAGGCGGCTGCATCACCTTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGCTATCCATCAGGTAACACGATGGTCCGATCGAGTCCGACGCATTACGGTTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGGTCGAAGGACATATGGATTGACCATTGTTCGCTGTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACGATTTCAAACAATTATTTCTCGCATCACGATGAGGTTATGCTGCTGGGTCACAGTGACACCTACTTGCCGGACTCCGGAATGCAGGTAACCATAGCATTCAACCACTTCGGGGAGAAGCTAGTGCAAAGGATGCCGCGATGCCGACGTGGCTACATCCACGTGGTCAACAACGACTTCACCCAATGGGAGATGTACGCAATCGGCGGCAGCGGAAACCCCACAATCAACAGCCAAGGCAACCGCTACACCGCGCCATACGACCGCAACGCCAAGGAGGTAACCAAGCGGGTCGAGACCGCCGAGTCCGAGTGGCGCGGCTGGAACTGGCGCTCCGACGGCGACATCCTCGTCAACGGCGCCTTCTTTGTCACTTCCGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAGTCCGCCGCGCTGATCGACCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGACAACAATTTGGGGATGTGGACCACTGGCCCTAATGGCGGAGGCTTTGCGATTGGGTCTGGCCCGGATTACACTGACGACATGTCGGAAAGCTGGTCGAGGTCTCGTTATTTTGTTATGGCGTTCTCGCTTTTGTTTTGTTCTTTTTGTGCATTGGCACTTGTCTTTGTTAGTTCTTAG

Protein sequence

MLATACIVLLALLLPSVPLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGFAIGSGPDYTDDMSESWSRSRYFVMAFSLLFCSFCALALVFVSS
Homology
[Close]
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 733.0 bits (1891), Expect = 2.1e-210
Identity = 362/480 (75.42%), Postives = 402/480 (83.75%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSVPLHGAAAALNHT-LPGQHPSPELVAQEVHRKVNASITRRQLLQ 60
           ML  +CIVL   L   VP     + LN T L   HP PELVA +V  KVNASITRRQ L 
Sbjct: 2   MLQRSCIVLFFSLFLLVP-QMVFSMLNRTLLLIPHPDPELVAYQVQWKVNASITRRQALD 61

Query: 61  LSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120
            +++  S+ C TGNPIDDCWKCDPNWPNNRQ LADC IGFGQYALGGK G+FY VTD SD
Sbjct: 62  TTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSD 121

Query: 121 DDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCI 180
           DD VNPKPGTLRY VIQ +PLWIVFP+NM+IKL QELIFNSYKTLDGRGANVHIVGGGCI
Sbjct: 122 DDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCI 181

Query: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240
           TLQY+SN+IIHNIHIHHCY SGNT VRSSPTHYG+RTKSDGDGISIFGSKDIWIDHCSLS
Sbjct: 182 TLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLS 241

Query: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQR 300
            CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD Y PDSGMQVTIAFNHFGEKL+QR
Sbjct: 242 RCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQR 301

Query: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESE 360
           MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAP +  AKEVTKRVET + +
Sbjct: 302 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGD 361

Query: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD 420
           W+GWNWRS+GDILVNGAFFV SG+G E++YEKAYSVEPKSA+ I Q+T+H+G LGVGGR+
Sbjct: 362 WKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLGVGGRN 421

Query: 421 NNLGMWTTGPNGGGFAIGSGPDYTDDMSESWSRSRYFVMAFSLL--FCSFCALALVFVSS 478
           NNLGMWTT  + G   + S  DYTD+MS + S +R   ++FS+L    S  +  +VF SS
Sbjct: 422 NNLGMWTTTGSEGTSGLDSYNDYTDEMSGAGSTNR---LSFSVLVFLLSSISYLVVFTSS 477

[Close]
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.5e-160
Identity = 292/478 (61.09%), Postives = 341/478 (71.34%), Query Frame = 0 

Query: 19  LHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQL------LQLSEKDESSSCYTG 78
           L  A   LN TLP QHPSP+ VA  V R VN S+ RRQL         S    SSSC TG
Sbjct: 19  LLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTG 78

Query: 79  NPIDDCWKC-DPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLR 138
           NPIDDCW+C D +W  NRQRLADC+IGFG   LGGKNG+ Y+VTD SD++P NP PGTLR
Sbjct: 79  NPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLR 138

Query: 139 YAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHN 198
           Y VIQ +PLWIVF +NMLI+L QELI NSYKTLDGRG+ VHI G GC+TLQY+ ++IIHN
Sbjct: 139 YGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHN 198

Query: 199 IHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMG 258
           +HI+ C PS           +  R +SDGDGISIFGS+ IW+DHCS+SHC DGLIDAVMG
Sbjct: 199 LHIYDCKPSAG---------FEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMG 258

Query: 259 STGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVV 318
           ST ITISNNYF+HHDEVMLLGH D Y PD+GMQVTIAFNHFG+ LVQRMPRCRRGYIHVV
Sbjct: 259 STAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVV 318

Query: 319 NNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETA-ESEWRGWNWRSDGD 378
           NNDFT+W+MYAIGGSGNPTINSQGNRY+AP D +AKEVTKRV++  + EW  WNWR++GD
Sbjct: 319 NNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGD 378

Query: 379 ILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD------NNLGM 438
           ++ NGAFFV SG+G+   Y KA SV+PK+A+L+DQLT +AG  G G RD      N+   
Sbjct: 379 LMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFG-GPRDDQGQSGNSYSP 438

Query: 439 WTTGPNGGGFAIGSGPDYTDDM--------SESWSRSRYFVMAFSLLFCSFCALALVF 475
           +     GGG + GS     D M        S S   S  F M F     S   L L+F
Sbjct: 439 YGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFGSDAPSRPRLTLLF 486

[Close]
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.6e-152
Identity = 250/385 (64.94%), Postives = 304/385 (78.96%), Query Frame = 0 

Query: 37  PELVAQEVHRKVNASITRRQLLQLSEKDESS-------SCYTGNPIDDCWKCDPNWPNNR 96
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CD NW  NR
Sbjct: 89  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 148

Query: 97  QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANML 156
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 149 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 208

Query: 157 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSP 216
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 209 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 268

Query: 217 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 276
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 269 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 328

Query: 277 LLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNP 336
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 329 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 388

Query: 337 TINSQGNRYTAPYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKY 396
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS+GD+L+NGAFF  SG G    Y
Sbjct: 389 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 448

Query: 397 EKAYSVEPKSAALIDQLTWHAGPLG 415
            +A S+  K ++++D +T  AG LG
Sbjct: 449 GRASSLAAKPSSMVDTITSTAGALG 463

[Close]
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.7e-151
Identity = 251/410 (61.22%), Postives = 306/410 (74.63%), Query Frame = 0 

Query: 6   CIVLLALLLPSVPLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQLSEKDE 65
           C +L + L  S+P+                 PELV +EVHRK+N SI+RR+L        
Sbjct: 37  CCILFSALASSLPV---------------SDPELVVEEVHRKINESISRRKL-------G 96

Query: 66  SSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNP 125
             SC +GNPIDDCW+CD +W  NR+RLADC IGFG+ A+GG++GE Y+VTD  +DDPVNP
Sbjct: 97  FFSCGSGNPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNP 156

Query: 126 KPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYIS 185
           +PGTLRYAVIQ +PLWI+F  +M I+L +ELI NS+KTLDGRGA+VHI GG CIT+QY++
Sbjct: 157 RPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVT 216

Query: 186 NVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGL 245
           N+IIH +HIH C   GNT VR SP HYGYRT SDGDG+SIFG   +W+DHCSLS+C DGL
Sbjct: 217 NIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGL 276

Query: 246 IDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRR 305
           IDA+ GST ITISNNY +HH++VMLLGHSDTY  D  MQVTIAFNHFGE LVQRMPRCR 
Sbjct: 277 IDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRH 336

Query: 306 GYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESEWRGWNW 365
           GY HVVNND+T WEMYAIGGS NPTINSQGNR+ AP D ++KEVTK  +  E EWR WNW
Sbjct: 337 GYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNW 396

Query: 366 RSDGDILVNGAFFVTSGQG--LEVKYEKAYSVEPKSAALIDQLTWHAGPL 414
           RS+GD+L+NGAFF  SG G      Y KA S+  + ++ + ++T  +G L
Sbjct: 397 RSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGAL 424

[Close]
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 536.6 bits (1381), Expect = 2.9e-151
Identity = 242/379 (63.85%), Postives = 296/379 (78.10%), Query Frame = 0 

Query: 35  PSPELVAQEVHRKVNASITRRQLLQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLAD 94
           P PE V +EVH+ +NAS+  R+ L         SC TGNPIDDCW+CDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 95  CAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQ 154
           CAIGFG+ A+GG++G  Y+VTD  +D+PV+PKPGTLR+AV+Q +PLWI+F  +M I+L +
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 155 ELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGY 214
           ELI NS+KT+DGRGA+VHI GG CIT+QY++N+IIH IHIH C   GN MVRSSP H+G+
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 215 RTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHS 274
           RT SDGDG+SIFG   +W+DHCS S+C+DGLIDA+MGST IT+SNN+ +HHD+VMLLGHS
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 275 DTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQ 334
           DTY  D  MQVTIAFNHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS NPTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 335 GNRYTAPYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYS 394
           GNR+ AP  R +KEVTK  +  ESEW+ WNWRS GD+L+NGAFF  SG      Y KA S
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

Query: 395 VEPKSAALIDQLTWHAGPL 414
           +  K ++L+  LT  +G L
Sbjct: 388 LGAKPSSLVGPLTSTSGAL 400

[Close]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match: A0A6J1EJ04 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.2e-266
Identity = 452/479 (94.36%), Postives = 457/479 (95.41%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQ 60
           ML T CIVLLALLLPS  PL  AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQLLQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQLLQ 60

Query: 61  LSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120
             EKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD
Sbjct: 61  SLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120

Query: 121 DDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCI 180
           DD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGGCI
Sbjct: 121 DDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCI 180

Query: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240
           TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS
Sbjct: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240

Query: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQR 300
           HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLVQR
Sbjct: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQR 300

Query: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESE 360
           MPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+ESE
Sbjct: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESE 360

Query: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD 420
           WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGGRD
Sbjct: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGRD 420

Query: 421 NNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVFV 476
           NNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL FV
Sbjct: 421 NNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSPYFVMVLSVLLCSFCALALAFV 479

[Close]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match: A0A6J1IRN3 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 4.5e-264
Identity = 450/480 (93.75%), Postives = 456/480 (95.00%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PL-HGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLL 60
           ML T CIVLLALLLPS  PL   AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQLL
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGASAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQLL 60

Query: 61  QLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS 120
           Q  EKDES SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS
Sbjct: 61  QSLEKDESPSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS 120

Query: 121 DDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGC 180
           DDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGGC
Sbjct: 121 DDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGC 180

Query: 181 ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSL 240
           ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKD+WIDHCSL
Sbjct: 181 ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDVWIDHCSL 240

Query: 241 SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQ 300
           SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLVQ
Sbjct: 241 SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQ 300

Query: 301 RMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAES 360
           RMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+ES
Sbjct: 301 RMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSES 360

Query: 361 EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGR 420
           EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGGR
Sbjct: 361 EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGR 420

Query: 421 DNNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVFV 476
           DNNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL FV
Sbjct: 421 DNNLGMWTTGPNGGGGFIIGSGPDYTDDMSESLSRIGSPYFVMVVSVLLCSFCALALAFV 480

[Close]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match: A0A0A0KIG0 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.0e-257
Identity = 441/482 (91.49%), Postives = 451/482 (93.57%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPS-VPLHGAA---AALNHTLPGQHPSPELVAQEVHRKVNASITRRQ 60
           MLAT CIVLLALLLPS +PL  AA   AALN TLPGQHPSPELVAQEVHRKVNAS+TRRQ
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60

Query: 61  LLQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQ+SEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD Y PDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETA 360
           VQRMPRCR GYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET+
Sbjct: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGP--NGGGFAIGSGPDYTDDMSESWSRSRYFVMAFSLLFCSFCALALVF 477
           GRDNNLGMWTTGP  NGGGF +GSG DYTDDMSES      FV  FS+L  SFC   +V 
Sbjct: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSES-----CFVRVFSVLLWSFCWALVVV 477

[Close]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match: A0A6J1I6R7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111471237 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.7e-256
Identity = 437/478 (91.42%), Postives = 447/478 (93.51%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSVPLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQL 60
           M+A  CIVLLALLL S  L     ALN TLPGQHPSPELVAQEVHRKVN S+TRRQL+Q 
Sbjct: 1   MVAAICIVLLALLLHS--LSPPCTALNLTLPGQHPSPELVAQEVHRKVNDSMTRRQLIQN 60

Query: 61  SEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDD 120
           SE DESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFY+VTDDSDD
Sbjct: 61  SENDESSSCYTGNPIDDCWKCDSNWPNNRQRLADCAIGFGQYALGGKNGEFYVVTDDSDD 120

Query: 121 DPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCIT 180
           DPVNP+PGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCIT
Sbjct: 121 DPVNPEPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCIT 180

Query: 181 LQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSH 240
           LQYISN+IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSH
Sbjct: 181 LQYISNIIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSH 240

Query: 241 CKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQRM 300
           CKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+Y PDSGMQVTIAFNHFGEKLVQRM
Sbjct: 241 CKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYSPDSGMQVTIAFNHFGEKLVQRM 300

Query: 301 PRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESEW 360
           PRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESEW
Sbjct: 301 PRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESEW 360

Query: 361 RGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRDN 420
           RGWNWRSDGDILVNGAFF+TSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG RDN
Sbjct: 361 RGWNWRSDGDILVNGAFFITSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGSRDN 420

Query: 421 NLGMWTTGPN-GGGFAIGSGPDYTDDMSES-WS-RSRYFVMAFSLLFCSFCALALVFV 476
           NLGMWTTG N GGG+ +GSGPDYTDDMSES W  R RYFVM F     SFCALAL FV
Sbjct: 421 NLGMWTTGSNGGGGYVLGSGPDYTDDMSESLWRIRFRYFVMVF-----SFCALALAFV 471

[Close]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match: A0A5A7VE54 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00400 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.5e-256
Identity = 441/485 (90.93%), Postives = 450/485 (92.78%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSVPLHGA------AAALNHTLPGQHPSPELVAQEVHRKVNASITR 60
           MLAT CIVLLALLLPS   H A      AAALN TLPGQHPSPELVAQEVHRKVNAS+TR
Sbjct: 1   MLATTCIVLLALLLPSRLPHRAGATTATAAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60

Query: 61  RQLLQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120
           RQLLQ+SEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV
Sbjct: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120

Query: 121 TDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180
           TDDSDDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV
Sbjct: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180

Query: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240
           GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID
Sbjct: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240

Query: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGE 300
           HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD Y PDSGMQVTIAFNHFGE
Sbjct: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300

Query: 301 KLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360
           KLVQRMPRCR GYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE
Sbjct: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360

Query: 361 TAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420
           T+ESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG
Sbjct: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420

Query: 421 VGGRDNNLGMWTTGP--NGGGFAIGSGPDYTDDMSESWSRSRYFVMAFSLL--FCSFCAL 476
           VGGRDNNLGMWTTGP  NGGGF +GSG DYTDDMS+S      FV AFS+L  FC    +
Sbjct: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSKS-----CFVRAFSVLLSFCWGLVV 480

[Close]
BLAST of Lcy04g011300 vs. NCBI nr
Match: KAG6604393.1 (putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 927.9 bits (2397), Expect = 3.4e-266
Identity = 452/479 (94.36%), Postives = 457/479 (95.41%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQ 60
           ML T CIVLLALLLPS  PL  AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQLLQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQLLQ 60

Query: 61  LSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120
             EKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD
Sbjct: 61  SLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120

Query: 121 DDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCI 180
           DD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGGCI
Sbjct: 121 DDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCI 180

Query: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240
           TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS
Sbjct: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240

Query: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQR 300
           HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLVQR
Sbjct: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQR 300

Query: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESE 360
           MPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+ESE
Sbjct: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESE 360

Query: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD 420
           WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGGRD
Sbjct: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGRD 420

Query: 421 NNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVFV 476
           NNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL FV
Sbjct: 421 NNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYFVMVLSVLLCSFCALALAFV 479

[Close]
BLAST of Lcy04g011300 vs. NCBI nr
Match: XP_022925720.1 (probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate lyase 12 [Cucurbita moschata])

HSP 1 Score: 927.5 bits (2396), Expect = 4.5e-266
Identity = 452/479 (94.36%), Postives = 457/479 (95.41%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PLHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQ 60
           ML T CIVLLALLLPS  PL  AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQLLQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQLLQ 60

Query: 61  LSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120
             EKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD
Sbjct: 61  SLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120

Query: 121 DDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCI 180
           DD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGGCI
Sbjct: 121 DDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCI 180

Query: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240
           TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS
Sbjct: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240

Query: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQR 300
           HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLVQR
Sbjct: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQR 300

Query: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESE 360
           MPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+ESE
Sbjct: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESE 360

Query: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD 420
           WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGGRD
Sbjct: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGRD 420

Query: 421 NNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVFV 476
           NNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL FV
Sbjct: 421 NNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSPYFVMVLSVLLCSFCALALAFV 479

[Close]
BLAST of Lcy04g011300 vs. NCBI nr
Match: XP_023544235.1 (probable pectate lyase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 452/481 (93.97%), Postives = 457/481 (95.01%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PL--HGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQL 60
           ML T CIVLLALLLPS  PL    AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQL
Sbjct: 1   MLTTTCIVLLALLLPSCSPLGASAAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQL 60

Query: 61  LQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDD 120
           LQ  EKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDD
Sbjct: 61  LQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDD 120

Query: 121 SDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGG 180
           SDDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGG
Sbjct: 121 SDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGG 180

Query: 181 CITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCS 240
           CITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCS
Sbjct: 181 CITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCS 240

Query: 241 LSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLV 300
           LSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLV
Sbjct: 241 LSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLV 300

Query: 301 QRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAE 360
           QRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+E
Sbjct: 301 QRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSE 360

Query: 361 SEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGG 420
           SEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGG
Sbjct: 361 SEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGG 420

Query: 421 RDNNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVF 476
           RDNNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL F
Sbjct: 421 RDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSPYFVMVLSVLLCSFCALALAF 480

[Close]
BLAST of Lcy04g011300 vs. NCBI nr
Match: XP_022978930.1 (probable pectate lyase 12 [Cucurbita maxima])

HSP 1 Score: 919.8 bits (2376), Expect = 9.3e-264
Identity = 450/480 (93.75%), Postives = 456/480 (95.00%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PL-HGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLL 60
           ML T CIVLLALLLPS  PL   AAAALN TLPGQHPSPELVAQEVHRKVNAS+TRRQLL
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGASAAAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQLL 60

Query: 61  QLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS 120
           Q  EKDES SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS
Sbjct: 61  QSLEKDESPSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDS 120

Query: 121 DDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGC 180
           DDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGGGC
Sbjct: 121 DDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGC 180

Query: 181 ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSL 240
           ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKD+WIDHCSL
Sbjct: 181 ITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDVWIDHCSL 240

Query: 241 SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQ 300
           SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLPDSGMQVTIAFNHFGEKLVQ
Sbjct: 241 SHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQ 300

Query: 301 RMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAES 360
           RMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVET+ES
Sbjct: 301 RMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSES 360

Query: 361 EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGR 420
           EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVGGR
Sbjct: 361 EWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGR 420

Query: 421 DNNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYFVMAFSLLFCSFCALALVFV 476
           DNNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YFVM  S+L CSFCALAL FV
Sbjct: 421 DNNLGMWTTGPNGGGGFIIGSGPDYTDDMSESLSRIGSPYFVMVVSVLLCSFCALALAFV 480

[Close]
BLAST of Lcy04g011300 vs. NCBI nr
Match: KAG7034543.1 (putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 452/499 (90.58%), Postives = 457/499 (91.58%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSV-PLHGAAAALNHTLPGQHPSPELVAQEVH-------------- 60
           ML T CIVLLALLLPS  PL  AAAALN TLPGQHPSPELVAQEVH              
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60

Query: 61  ------RKVNASITRRQLLQLSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVNAS+TRRQLLQ  EKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF
Sbjct: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFN 180
           GQYALGGKNGEFYIVTDDSDDD VNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFN
Sbjct: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+YLP
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVET+ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 AALIDQLTWHAGPLGVGGRDNNLGMWTTGPN-GGGFAIGSGPDYTDDMSESWSR--SRYF 476
            ALIDQLTWHAGPLGVGGRDNNLGMWTTGPN GGGF IGSGPDYTDDMSES SR  S YF
Sbjct: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480

[Close]
BLAST of Lcy04g011300 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.5e-211
Identity = 362/480 (75.42%), Postives = 402/480 (83.75%), Query Frame = 0 

Query: 1   MLATACIVLLALLLPSVPLHGAAAALNHT-LPGQHPSPELVAQEVHRKVNASITRRQLLQ 60
           ML  +CIVL   L   VP     + LN T L   HP PELVA +V  KVNASITRRQ L 
Sbjct: 2   MLQRSCIVLFFSLFLLVP-QMVFSMLNRTLLLIPHPDPELVAYQVQWKVNASITRRQALD 61

Query: 61  LSEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSD 120
            +++  S+ C TGNPIDDCWKCDPNWPNNRQ LADC IGFGQYALGGK G+FY VTD SD
Sbjct: 62  TTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSD 121

Query: 121 DDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCI 180
           DD VNPKPGTLRY VIQ +PLWIVFP+NM+IKL QELIFNSYKTLDGRGANVHIVGGGCI
Sbjct: 122 DDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCI 181

Query: 181 TLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLS 240
           TLQY+SN+IIHNIHIHHCY SGNT VRSSPTHYG+RTKSDGDGISIFGSKDIWIDHCSLS
Sbjct: 182 TLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLS 241

Query: 241 HCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQR 300
            CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD Y PDSGMQVTIAFNHFGEKL+QR
Sbjct: 242 RCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQR 301

Query: 301 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETAESE 360
           MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAP +  AKEVTKRVET + +
Sbjct: 302 MPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGD 361

Query: 361 WRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD 420
           W+GWNWRS+GDILVNGAFFV SG+G E++YEKAYSVEPKSA+ I Q+T+H+G LGVGGR+
Sbjct: 362 WKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLGVGGRN 421

Query: 421 NNLGMWTTGPNGGGFAIGSGPDYTDDMSESWSRSRYFVMAFSLL--FCSFCALALVFVSS 478
           NNLGMWTT  + G   + S  DYTD+MS + S +R   ++FS+L    S  +  +VF SS
Sbjct: 422 NNLGMWTTTGSEGTSGLDSYNDYTDEMSGAGSTNR---LSFSVLVFLLSSISYLVVFTSS 477

[Close]
BLAST of Lcy04g011300 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 591.7 bits (1524), Expect = 5.4e-169
Identity = 284/440 (64.55%), Postives = 336/440 (76.36%), Query Frame = 0 

Query: 6   CIVLLALLLPSVPLHG-----AAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQLLQL 65
           CI+   LL+ S+  HG     +A+  N +LP QHP PE V   V RK+N S++RRQLL  
Sbjct: 18  CIIWFCLLV-SLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQRKLNDSLSRRQLLTY 77

Query: 66  SEKDESS------SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 125
            + D ++      SC TGNPIDDCW+CDPNW  NRQRLADC+IGFGQ  LGGK G+FY+V
Sbjct: 78  QQDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADCSIGFGQGTLGGKGGQFYLV 137

Query: 126 TDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 185
           TD SD+D  NP PGTLR+AVIQP+PLWI+F ++M IKL  ELI  SYKT+DGRG N+ I 
Sbjct: 138 TDSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHELIIGSYKTIDGRGTNIQIT 197

Query: 186 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 245
           G GC+T+Q +S+VIIHN+HIHHC PSGNT+V SSPTH G+R  SDGDGIS+  S  IW+D
Sbjct: 198 GHGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFRGVSDGDGISVSASHHIWVD 257

Query: 246 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGE 305
           HCSL +C DGLID ++ ST +TISNNYFSHHDEVMLLGH D Y  D GMQVTIAFNHFGE
Sbjct: 258 HCSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDDRYTADKGMQVTIAFNHFGE 317

Query: 306 KLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 365
            LVQRMPRCR GYIHVVNNDFT WEMYAIGGS +PTINSQGNRYTAP D NAKEVTKRV+
Sbjct: 318 GLVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQGNRYTAPIDPNAKEVTKRVD 377

Query: 366 TAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 425
           + E  W GWNWR++GD++VNGAFFV SG G+   Y +A SV+PK+AA+IDQLT +AG  G
Sbjct: 378 SNEKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFG 437

Query: 426 -VGGRDNNLGMWTTGPNGGG 434
              GR+   G +    NGGG
Sbjct: 438 DPSGRNGQGGSFPGITNGGG 456

[Close]
BLAST of Lcy04g011300 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 567.4 bits (1461), Expect = 1.1e-161
Identity = 292/478 (61.09%), Postives = 341/478 (71.34%), Query Frame = 0 

Query: 19  LHGAAAALNHTLPGQHPSPELVAQEVHRKVNASITRRQL------LQLSEKDESSSCYTG 78
           L  A   LN TLP QHPSP+ VA  V R VN S+ RRQL         S    SSSC TG
Sbjct: 19  LLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTG 78

Query: 79  NPIDDCWKC-DPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLR 138
           NPIDDCW+C D +W  NRQRLADC+IGFG   LGGKNG+ Y+VTD SD++P NP PGTLR
Sbjct: 79  NPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLR 138

Query: 139 YAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHN 198
           Y VIQ +PLWIVF +NMLI+L QELI NSYKTLDGRG+ VHI G GC+TLQY+ ++IIHN
Sbjct: 139 YGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHN 198

Query: 199 IHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMG 258
           +HI+ C PS           +  R +SDGDGISIFGS+ IW+DHCS+SHC DGLIDAVMG
Sbjct: 199 LHIYDCKPSAG---------FEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMG 258

Query: 259 STGITISNNYFSHHDEVMLLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVV 318
           ST ITISNNYF+HHDEVMLLGH D Y PD+GMQVTIAFNHFG+ LVQRMPRCRRGYIHVV
Sbjct: 259 STAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVV 318

Query: 319 NNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETA-ESEWRGWNWRSDGD 378
           NNDFT+W+MYAIGGSGNPTINSQGNRY+AP D +AKEVTKRV++  + EW  WNWR++GD
Sbjct: 319 NNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGD 378

Query: 379 ILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRD------NNLGM 438
           ++ NGAFFV SG+G+   Y KA SV+PK+A+L+DQLT +AG  G G RD      N+   
Sbjct: 379 LMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFG-GPRDDQGQSGNSYSP 438

Query: 439 WTTGPNGGGFAIGSGPDYTDDM--------SESWSRSRYFVMAFSLLFCSFCALALVF 475
           +     GGG + GS     D M        S S   S  F M F     S   L L+F
Sbjct: 439 YGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFGSDAPSRPRLTLLF 486

[Close]
BLAST of Lcy04g011300 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.9e-153
Identity = 250/385 (64.94%), Postives = 304/385 (78.96%), Query Frame = 0 

Query: 37  PELVAQEVHRKVNASITRRQLLQLSEKDESS-------SCYTGNPIDDCWKCDPNWPNNR 96
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CD NW  NR
Sbjct: 89  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 148

Query: 97  QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANML 156
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 149 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 208

Query: 157 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSP 216
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 209 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 268

Query: 217 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 276
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 269 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 328

Query: 277 LLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNP 336
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 329 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 388

Query: 337 TINSQGNRYTAPYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKY 396
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS+GD+L+NGAFF  SG G    Y
Sbjct: 389 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 448

Query: 397 EKAYSVEPKSAALIDQLTWHAGPLG 415
            +A S+  K ++++D +T  AG LG
Sbjct: 449 GRASSLAAKPSSMVDTITSTAGALG 463

[Close]
BLAST of Lcy04g011300 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.9e-153
Identity = 250/385 (64.94%), Postives = 304/385 (78.96%), Query Frame = 0 

Query: 37  PELVAQEVHRKVNASITRRQLLQLSEKDESS-------SCYTGNPIDDCWKCDPNWPNNR 96
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CD NW  NR
Sbjct: 70  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 129

Query: 97  QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDPVNPKPGTLRYAVIQPQPLWIVFPANML 156
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 130 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 189

Query: 157 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSP 216
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 190 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 249

Query: 217 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 276
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 250 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 309

Query: 277 LLGHSDTYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNP 336
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 310 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 369

Query: 337 TINSQGNRYTAPYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKY 396
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS+GD+L+NGAFF  SG G    Y
Sbjct: 370 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 429

Query: 397 EKAYSVEPKSAALIDQLTWHAGPLG 415
            +A S+  K ++++D +T  AG LG
Sbjct: 430 GRASSLAAKPSSMVDTITSTAGALG 444

The following BLAST results are available for this feature:
BLAST of Lcy04g011300 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SCP22.1e-21075.42Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2[more]
Q93Z041.5e-16061.09Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1[more]
Q944R12.6e-15264.94Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q93Z251.7e-15161.22Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Q9C5M82.9e-15163.85Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
BLAST of Lcy04g011300 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EJ042.2e-26694.36Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1[more]
A0A6J1IRN34.5e-26493.75Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1[more]
A0A0A0KIG07.0e-25791.49Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1[more]
A0A6J1I6R72.7e-25691.42Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111471237 PE=3 SV=1[more]
A0A5A7VE543.5e-25690.93Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00400 ... [more]
BLAST of Lcy04g011300 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6604393.13.4e-26694.36putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925720.14.5e-26694.36probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate ... [more]
XP_023544235.11.1e-26493.97probable pectate lyase 12 [Cucurbita pepo subsp. pepo][more]
XP_022978930.19.3e-26493.75probable pectate lyase 12 [Cucurbita maxima][more]
KAG7034543.11.3e-26290.58putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Lcy04g011300 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G53190.11.5e-21175.42Pectin lyase-like superfamily protein [more]
AT5G04310.15.4e-16964.55Pectin lyase-like superfamily protein [more]
AT3G54920.11.1e-16161.09Pectin lyase-like superfamily protein [more]
AT4G13710.11.9e-15364.94Pectin lyase-like superfamily protein [more]
AT4G13710.21.9e-15364.94Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 119..144
score: 67.95
coord: 95..112
score: 57.67
coord: 299..318
score: 68.1
coord: 155..171
score: 67.79
coord: 219..240
score: 67.53
coord: 363..387
score: 50.67
coord: 321..340
score: 73.33
coord: 392..415
score: 27.38
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 147..344
e-value: 2.5E-93
score: 326.0
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 157..336
e-value: 4.9E-22
score: 78.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 72..414
e-value: 2.3E-117
score: 393.8
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 2..433
NoneNo IPR availablePANTHERPTHR31683:SF21PECTATE LYASE 12-RELATEDcoord: 2..433
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 68..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g011300.1Lcy04g011300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity