Lcy04g005940 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g005940
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand family protein
Location	Chr04: 34979242 .. 34979835 (-)
RNA-Seq Expression	Lcy04g005940
Synteny	Lcy04g005940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAACCACTCCGCCGCCGCCGATCTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTGCGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCAATTCCGATCACAAGGACAACGACGGCGTCGTCTCCAGGAAGGAGTTGGAGGCGATTCTCTGCCGGATCGCGTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGAATCACCGCGGAGGAGCTCCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

mRNA sequence

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAACCACTCCGCCGCCGCCGATCTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTGCGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCAATTCCGATCACAAGGACAACGACGGCGTCGTCTCCAGGAAGGAGTTGGAGGCGATTCTCTGCCGGATCGCGTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGAATCACCGCGGAGGAGCTCCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAACCACTCCGCCGCCGCCGATCTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTGCGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCAATTCCGATCACAAGGACAACGACGGCGTCGTCTCCAGGAAGGAGTTGGAGGCGATTCTCTGCCGGATCGCGTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGAATCACCGCGGAGGAGCTCCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

Protein sequence

MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSSSENSFKSHPAAGCGTPTSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAEFVRMMELQR

Homology

[Close]

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 7.3e-40
Identity = 109/206 (52.91%), Postives = 136/206 (66.02%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSS--SENSFKSHPAAGCGTPTSVLP 65
           R SPKRLFR+K    + A +SR +P SF S  SSSSS  S  + K  P A   TP SVLP
Sbjct: 9   RLSPKRLFRTK----SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLP 68

Query: 66  ADWSDSNSD--------HKDNDGVVSRKELEAILCRIA----SEEEVAMMLSEVDG--GG 125
            +  D  ++         +D+DGVVSR +L A++ R++    S+EEV++ML EVDG  GG
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMAR-AGPAFEPAGES-ELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLE 185
            I LE L +R AG + E + E+ ELRE FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFAEFVRMM 194
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.6e-39
Identity = 109/215 (50.70%), Postives = 138/215 (64.19%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSSSENSFKSHPAAGCG--TP 60
           MK+ K   PKR F   K+ S  +      P +F  G++SSSS ++   S    G G  TP
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSK----SPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLPADWSDSNSDH---------KDNDGVVSRKELEAILCRIA----SEEEVAMMLSEV 120
           TS+LP   S  +            +DNDG VSR +LE++L R+     +EEE+ +ML EV
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 --DGGGFIRLEALMARAGPAFEPAGES-ELRETFEFFDADHDGRITAEELHGVFSAIGDE 180
             DG G IRLE L +R   + +PA +S EL+ETFEFFDAD DG I+A+EL  VFS IGDE
Sbjct: 121 DCDGDGTIRLEELASRV-VSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDE 180

Query: 181 RCTLEDCRRMIADVDENGDGFVCFAEFVRMMELQR 198
           RCTL+DC+RMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 RCTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-18
Identity = 53/128 (41.41%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 74  KDNDGVVSRKELEAILCRI---ASEEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG 133
           K+NDG +S  EL+ ++  +   AS+EE   M+ E  +DG GFI L+  +A    + + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 134 ES---ELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVC 193
            S   +L+E F+ +D D +GRI+A ELH V   +G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 8.7e-17
Identity = 53/130 (40.77%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 74  KDNDGVVSRKELEAI---LCRIASEEEVAMMLS--EVDGGGFIRLEALMARAGPAFEPAG 133
           K+ DG +S  EL+ +   L   AS EE   M+   ++DG GFI L+  +A         G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 134 E-----SELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGF 193
                 S+L+E FE +D D +GRI+A+ELH V   +G E+C+++DC++MI+ VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

[Close]

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.3e-16
Identity = 64/163 (39.26%), Postives = 87/163 (53.37%), Query Frame = 0


Query: 39  SSSSSENSFKSHPAAGCGTPTSVLPADWSDSNSDHKDN-DGVVSRKELEAI---LCRIAS 98
           S S S    ++ PA+G G+P      +          N DG +SR EL A+   L   A+
Sbjct: 14  SGSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAAT 73

Query: 99  EEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG--ESELRETFEFFDADHDGRITAE 158
           ++E+A M++E   DG GFI L+   A    A   A   E +LR  F  FDAD +G I+A 
Sbjct: 74  DDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAA 133

Query: 159 ELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAEFVRMM 194
           EL  V   +G E+ T++ CRRMI  VD+NGDG + F EF  MM
Sbjct: 134 ELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 162/198 (81.82%), Postives = 172/198 (86.87%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN S  A++SR DPPSFGSGT SSSSSSEN FK H  AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--AEVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++NDGVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESELRETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 198
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 6.1e-74
Identity = 159/198 (80.30%), Postives = 169/198 (85.35%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN S  AD+SR DPPSFGSGT SSSSSSEN FK H  AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL      S+SD ++ND VVSRKELEAIL RIASEEEVAMMLSEVD GGFIRL+ LMAR
Sbjct: 61  SVLA---GCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESELRETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 198
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.1e-70
Identity = 158/201 (78.61%), Postives = 167/201 (83.08%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPA-AGCGTP 60
           MKIPKRFSPKRLFRSKK+HS  AD+SR DPPSFGSGT SSSSSSEN FK++    G GTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHHS--ADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTP 61

Query: 61  TSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL       +SD + N+GVVSRKELEAIL RIASEEEVAMML EVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESE+RETFEFFDAD DGRITAEELHGVF +IGDERCTLEDC RMI DV
Sbjct: 122 MARVGSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 198
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 5.4e-70
Identity = 158/201 (78.61%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPA-AGCGTP 60
           MKIPKRFSPKRLFRSKK+ S  AD+SR DPPSFGSGT SSSSSSEN FK +    G GTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHQS--ADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTP 61

Query: 61  TSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL       +SD ++N+GVV+RKELEAIL RIASEEEVAMMLSEVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESELRETFEFFDAD DGRITAEELHGVF +IGDE+CTLEDCRRMI DV
Sbjct: 122 MARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 198
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 5.4e-70
Identity = 158/201 (78.61%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPA-AGCGTP 60
           MKIPKRFSPKRLFRSKK+ S  AD+SR DPPSFGSGT SSSSSSEN FK +    G GTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHQS--ADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTP 61

Query: 61  TSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL       +SD ++N+GVV+RKELEAIL RIASEEEVAMMLSEVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESELRETFEFFDAD DGRITAEELHGVF +IGDE+CTLEDCRRMI DV
Sbjct: 122 MARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 198
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 295.0 bits (754), Expect = 4.7e-76
Identity = 162/198 (81.82%), Postives = 172/198 (86.87%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN S  A++SR DPPSFGSGT SSSSSSEN FK H  AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--AEVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++NDGVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESELRETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 198
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lcy04g005940 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 294.3 bits (752), Expect = 7.9e-76
Identity = 162/198 (81.82%), Postives = 171/198 (86.36%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN S  AD+SR DPPSFGSGT SSSSSSEN FK H  AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++NDGVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+   ESELRETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 198
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lcy04g005940 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 287.0 bits (733), Expect = 1.3e-73
Identity = 159/198 (80.30%), Postives = 169/198 (85.35%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN S  AD+SR DPPSFGSGT SSSSSSEN FK H  AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL      S+SD ++ND VVSRKELEAIL RIASEEEVAMMLSEVD GGFIRL+ LMAR
Sbjct: 61  SVLA---GCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESELRETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 198
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. NCBI nr
Match: XP_038883626.1 (probable calcium-binding protein CML35 [Benincasa hispida])

HSP 1 Score: 277.3 bits (708), Expect = 1.0e-70
Identity = 156/203 (76.85%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSSSENSF--KSHPAAGCGTP 60
           MKIPKRFSPKRLFRSKK HS  AD+SR DPPSFGSGTSSSSSS  +F   +  A G GTP
Sbjct: 1   MKIPKRFSPKRLFRSKKYHS--ADVSRSDPPSFGSGTSSSSSSSETFFKPNATATGYGTP 60

Query: 61  TSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGG-----GFIRL 120
           TSVLP DW DS    ++NDGVVSRKELEAIL RIASEEEVAMMLSEV  G     GFI L
Sbjct: 61  TSVLPPDWPDSG--QRENDGVVSRKELEAILSRIASEEEVAMMLSEVSSGGGGGDGFIWL 120

Query: 121 EALMARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMI 180
           E LMA+ G   E AGESELRETFEFFDAD DGRITAEELH VF +IGDE+CTLEDCRRMI
Sbjct: 121 EELMAQVGTGVEAAGESELRETFEFFDADQDGRITAEELHEVFRSIGDEQCTLEDCRRMI 180

Query: 181 ADVDENGDGFVCFAEFVRMMELQ 197
            DVDENGDG VCFAEFVRMMELQ
Sbjct: 181 RDVDENGDGVVCFAEFVRMMELQ 199

[Close]

BLAST of Lcy04g005940 vs. NCBI nr
Match: XP_004151276.1 (probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetical protein Csa_012346 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 8.5e-70
Identity = 158/201 (78.61%), Postives = 167/201 (83.08%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGT-SSSSSSENSFKSHPA-AGCGTP 60
           MKIPKRFSPKRLFRSKK+HS  AD+SR DPPSFGSGT SSSSSSEN FK++    G GTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHHS--ADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTP 61

Query: 61  TSVLPADWSDSNSDHKDNDGVVSRKELEAILCRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL       +SD + N+GVVSRKELEAIL RIASEEEVAMML EVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESE+RETFEFFDAD DGRITAEELHGVF +IGDERCTLEDC RMI DV
Sbjct: 122 MARVGSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 198
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lcy04g005940 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 165.2 bits (417), Expect = 5.2e-41
Identity = 109/206 (52.91%), Postives = 136/206 (66.02%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSS--SENSFKSHPAAGCGTPTSVLP 65
           R SPKRLFR+K    + A +SR +P SF S  SSSSS  S  + K  P A   TP SVLP
Sbjct: 9   RLSPKRLFRTK----SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLP 68

Query: 66  ADWSDSNSD--------HKDNDGVVSRKELEAILCRIA----SEEEVAMMLSEVDG--GG 125
            +  D  ++         +D+DGVVSR +L A++ R++    S+EEV++ML EVDG  GG
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMAR-AGPAFEPAGES-ELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLE 185
            I LE L +R AG + E + E+ ELRE FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFAEFVRMM 194
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lcy04g005940 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.2e-40
Identity = 109/215 (50.70%), Postives = 138/215 (64.19%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSAAADLSRCDPPSFGSGTSSSSSSENSFKSHPAAGCG--TP 60
           MK+ K   PKR F   K+ S  +      P +F  G++SSSS ++   S    G G  TP
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSK----SPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLPADWSDSNSDH---------KDNDGVVSRKELEAILCRIA----SEEEVAMMLSEV 120
           TS+LP   S  +            +DNDG VSR +LE++L R+     +EEE+ +ML EV
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 --DGGGFIRLEALMARAGPAFEPAGES-ELRETFEFFDADHDGRITAEELHGVFSAIGDE 180
             DG G IRLE L +R   + +PA +S EL+ETFEFFDAD DG I+A+EL  VFS IGDE
Sbjct: 121 DCDGDGTIRLEELASRV-VSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDE 180

Query: 181 RCTLEDCRRMIADVDENGDGFVCFAEFVRMMELQR 198
           RCTL+DC+RMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 RCTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lcy04g005940 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 93.2 bits (230), Expect = 2.5e-19
Identity = 53/128 (41.41%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 74  KDNDGVVSRKELEAILCRI---ASEEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG 133
           K+NDG +S  EL+ ++  +   AS+EE   M+ E  +DG GFI L+  +A    + + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 134 ES---ELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVC 193
            S   +L+E F+ +D D +GRI+A ELH V   +G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of Lcy04g005940 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 88.6 bits (218), Expect = 6.2e-18
Identity = 53/130 (40.77%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 74  KDNDGVVSRKELEAI---LCRIASEEEVAMMLS--EVDGGGFIRLEALMARAGPAFEPAG 133
           K+ DG +S  EL+ +   L   AS EE   M+   ++DG GFI L+  +A         G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 134 E-----SELRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGF 193
                 S+L+E FE +D D +GRI+A+ELH V   +G E+C+++DC++MI+ VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

[Close]

BLAST of Lcy04g005940 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 81.6 bits (200), Expect = 7.5e-16
Identity = 48/127 (37.80%), Postives = 74/127 (58.27%), Query Frame = 0


Query: 75  DNDGVVSRKELEAILCRI---ASEEEVAMMLSEVD--GGGFIRLEALMARAGPAFEPAGE 134
           + DG +S KEL AI+  +     EEE+   ++E+D  G G+I  E  +       +    
Sbjct: 48  NGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDV 107

Query: 135 SE-LRETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAE 194
            E L++ F  +D D +G I+AEELH V  ++GDE C++ +CR+MI  VD++GDG + F E
Sbjct: 108 LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEE 167

Query: 195 FVRMMEL 196
           F  MM +
Sbjct: 168 FKIMMTM 173

The following BLAST results are available for this feature:

BLAST of Lcy04g005940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30188	7.3e-40	52.91	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9SS31	1.6e-39	50.70	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
Q9C8Y1	3.5e-18	41.41	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	8.7e-17	40.77	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q8RZB5	3.3e-16	39.26	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lcy04g005940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F310	2.3e-76	81.82	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	6.1e-74	80.30	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A0A0L0H1	4.1e-70	78.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]
A0A5A7VA05	5.4e-70	78.61	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	5.4e-70	78.61	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]

BLAST of Lcy04g005940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932888.1	4.7e-76	81.82	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_023543601.1	7.9e-76	81.82	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_022967907.1	1.3e-73	80.30	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_038883626.1	1.0e-70	76.85	probable calcium-binding protein CML35 [Benincasa hispida]	[more]
XP_004151276.1	8.5e-70	78.61	probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetica...	[more]

BLAST of Lcy04g005940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41410.1	5.2e-41	52.91	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	1.2e-40	50.70	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	2.5e-19	41.41	calmodulin like 23	[more]
AT5G37770.1	6.2e-18	40.77	EF hand calcium-binding protein family	[more]
AT1G24620.1	7.5e-16	37.80	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 131..159 e-value: 2.9E-5 score: 33.5 coord: 168..196 e-value: 4.4E-5 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 130..193 e-value: 1.4E-9 score: 38.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 164..197 score: 12.7281
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 14.569197
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..193 e-value: 1.10818E-18 score: 74.1213
None	No IPR available	GENE3D	1.10.238.10		coord: 70..194 e-value: 1.0E-24 score: 89.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..78
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..53
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..195
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..195
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 177..189
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 74..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g005940.1	Lcy04g005940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding