Lcy04g004630 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g004630
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	polygalacturonase inhibitor-like
Location	Chr04: 30819106 .. 30820261 (+)
RNA-Seq Expression	Lcy04g004630
Synteny	Lcy04g004630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGATAATTCATCAAAACACACACTTCAACAAAAAAAATGCATCCCTTCACTTTGTTCCTCGTCCTCTTGCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGGCCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGTACTGCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCACCGACGAGGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTCCCATTCCTCCAAACCCTGGTGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCAACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTGAGCTGGACCAATATCTCCGGCTCCGTCCCCGATTTCCTCAGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACCCTCCCCGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTCCAATTGGACCGAAACAAATTGACAGGGCCCATCCCAGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCCTCGACTTGTCCAGGAACAAGCTGGAAGGCGATGCCTCCATGGTATTCGGAGCCCAGAAGAAGGCGTGGAAAGTCGATCTGTCGAGGAATTTGTTGTCGTTTGATATGTCGAAGGTTGTGTTCTCGACGAGCCTGTTTCATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACGAAACTGAGTAATCTGAATTCTTTCAATGTGAGTTATAATCGGCTGTGCGGTCCGATCCCGCAGGGTGGGGAGTTGCAGAGCTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCAGCTGCAAATCATGATTTCTCTTTCTCCTATGGAATTTCAAGTTCCTATTAAATAAAAGTTACCAACTTTTCTACAAAAGTATATTGCACTAACTTTAAATTCATGTTGGCATATATATATCCCCATCCAGAAATCAAACGTGAAA

mRNA sequence

CTCGATAATTCATCAAAACACACACTTCAACAAAAAAAATGCATCCCTTCACTTTGTTCCTCGTCCTCTTGCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGGCCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGTACTGCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCACCGACGAGGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTCCCATTCCTCCAAACCCTGGTGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCAACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTGAGCTGGACCAATATCTCCGGCTCCGTCCCCGATTTCCTCAGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACCCTCCCCGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTCCAATTGGACCGAAACAAATTGACAGGGCCCATCCCAGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCCTCGACTTGTCCAGGAACAAGCTGGAAGGCGATGCCTCCATGGTATTCGGAGCCCAGAAGAAGGCGTGGAAAGTCGATCTGTCGAGGAATTTGTTGTCGTTTGATATGTCGAAGGTTGTGTTCTCGACGAGCCTGTTTCATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACGAAACTGAGTAATCTGAATTCTTTCAATGTGAGTTATAATCGGCTGTGCGGTCCGATCCCGCAGGGTGGGGAGTTGCAGAGCTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCAGCTGCAAATCATGATTTCTCTTTCTCCTATGGAATTTCAAGTTCCTATTAAATAAAAGTTACCAACTTTTCTACAAAAGTATATTGCACTAACTTTAAATTCATGTTGGCATATATATATCCCCATCCAGAAATCAAACGTGAAA

Coding sequence (CDS)

ATGCATCCCTTCACTTTGTTCCTCGTCCTCTTGCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGGCCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGTACTGCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCACCGACGAGGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTCCCATTCCTCCAAACCCTGGTGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCAACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTGAGCTGGACCAATATCTCCGGCTCCGTCCCCGATTTCCTCAGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACCCTCCCCGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTCCAATTGGACCGAAACAAATTGACAGGGCCCATCCCAGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCCTCGACTTGTCCAGGAACAAGCTGGAAGGCGATGCCTCCATGGTATTCGGAGCCCAGAAGAAGGCGTGGAAAGTCGATCTGTCGAGGAATTTGTTGTCGTTTGATATGTCGAAGGTTGTGTTCTCGACGAGCCTGTTTCATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACGAAACTGAGTAATCTGAATTCTTTCAATGTGAGTTATAATCGGCTGTGCGGTCCGATCCCGCAGGGTGGGGAGTTGCAGAGCTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCAGCTGCAAATCATGA

Protein sequence

MHPFTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSFDVYSYFHNKCLCGAPLPSCKS

Homology

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BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.7e-114
Identity = 204/328 (62.20%), Postives = 245/328 (74.70%), Query Frame = 0


Query: 4   FTLFLVLLLSFTI----SFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVE 63
           F+ FL L L F+     + ++ CNP+DKK LL+IKKAF DPYVLASWK + DCC WYCV 
Sbjct: 5   FSTFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVT 64

Query: 64  CGEKSDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKF 123
           C   ++RI ++ I    ++SG IP  VGDLP+L+TL   K PNL GP+QPAIAKL  LK 
Sbjct: 65  CDSTTNRINSLTIFAG-QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKS 124

Query: 124 LDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPI 183
           L +SWTN+SGSVPDFLS L NL ++ L+FNNLTG +P+SLS+LP LGAL+LDRNKLTG I
Sbjct: 125 LRLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHI 184

Query: 184 PDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWK 243
           P SFG        L LSHN+LSG IP S  ++D  S+DLSRNKLEGDAS++FG  K    
Sbjct: 185 PISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQI 244

Query: 244 VDLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIP 303
           VDLSRNLL F++SKV F TSL  LD+NHNKI+G +P + T+L N    NVSYNRLCG IP
Sbjct: 245 VDLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIP 304

Query: 304 QGGELQSFDVYSYFHNKCLCGAPLPSCK 328
            GG+LQSFD YSYFHN+CLCGAPLPSCK
Sbjct: 305 VGGKLQSFDEYSYFHNRCLCGAPLPSCK 330

[Close]

BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.0e-107
Identity = 199/324 (61.42%), Postives = 232/324 (71.60%), Query Frame = 0


Query: 5   TLFLVLLLSFTI-SFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEK 64
           +L LVLL +    S +ERCNP DKK LL+IKKA ++PY+LASW P  DCC WYCVEC   
Sbjct: 12  SLLLVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLT 71

Query: 65  SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVS 124
           + RI ++ I +  +LSG IP AVGDLPFL+TL+ RKL NL G + PAIAKL +LK + +S
Sbjct: 72  THRINSLTIFSG-QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLS 131

Query: 125 WTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 184
           WTN+SG VP F S L NL Y+ L+FNNL+G +P SLS LP LGAL LDRN LTGPIPDSF
Sbjct: 132 WTNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSF 191

Query: 185 GYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLS 244
           G     +  L LSHN+LSGKIP S    D   +DLSRNKLEGD S+ F A K    VD S
Sbjct: 192 GKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFS 251

Query: 245 RNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGE 304
           RNL  FD+S+V F  SL  LDL+HNKI G LP  MT L +L   NVSYNRLCG IP GG+
Sbjct: 252 RNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSL-DLQFLNVSYNRLCGKIPVGGK 311

Query: 305 LQSFDVYSYFHNKCLCGAPLPSCK 328
           LQSFD  SYFHN+CLCGAPL SCK
Sbjct: 312 LQSFDYDSYFHNRCLCGAPLQSCK 333

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BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.8e-105
Identity = 187/327 (57.19%), Postives = 239/327 (73.09%), Query Frame = 0


Query: 5   TLFLVLLLSF--TISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGE 64
           TL L+ L +F  T    + CN NDK  LL+IKK+ N+PY LASW P+ DCC+WYC+ECG+
Sbjct: 6   TLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGD 65

Query: 65  K--SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFL 124
              + R+  + I +  ++SG IP  VGDLP+L+TLV RKL NL G +QP IAKL NL+ L
Sbjct: 66  ATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRML 125

Query: 125 DVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIP 184
            +SWTN++G +PDF+S L NLE++ L+FN+L+G++P+SLS LPK+ AL+L RNKLTG IP
Sbjct: 126 RLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIP 185

Query: 185 DSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKV 244
           +SFG        L LSHN+LSG IP SL  +D   +DLSRNKL+GDASM+FG+ K  W +
Sbjct: 186 ESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 245

Query: 245 DLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQ 304
           DLSRN+  FD+SKV    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP 
Sbjct: 246 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPT 305

Query: 305 GGELQSFDVYSYFHNKCLCGAPLPSCK 328
           GG+LQ+FD YSYFHNKCLCGAPL  CK
Sbjct: 306 GGKLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 4.8e-105
Identity = 188/326 (57.67%), Postives = 241/326 (73.93%), Query Frame = 0


Query: 5   TLFLVL--LLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGE 64
           TLFL+L  LL  T    + C+ +DK  LL+IKK+ N+PY LASW P+ DCC+WYC+ECG+
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 65  KS-DRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLD 124
            + +  +T  I  D E+SG IPP VGDLP+L +L+ RKL NL G +QP IAKL NL FL 
Sbjct: 66  ATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 125

Query: 125 VSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPD 184
           +SWTN++G VP+FLS L NLEYI L+FN+L+G++P+SLS L KL  L+L RNKLTGPIP+
Sbjct: 126 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 185

Query: 185 SFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVD 244
           SFG    +   L LSHN+LSG IP SL   D + +DLSRNKL+GDAS++FGA+K  W VD
Sbjct: 186 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 245

Query: 245 LSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQG 304
           +SRN+  FD+SKV  + +L +LD+NHN I G +P + +K +     NVSYNRLCG IP+G
Sbjct: 246 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKG 305

Query: 305 GELQSFDVYSYFHNKCLCGAPLPSCK 328
             +Q FD YS+FHNKCLCGAPLPSCK
Sbjct: 306 EYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot
Match: P35334 (Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.3e-85
Identity = 156/327 (47.71%), Postives = 220/327 (67.28%), Query Frame = 0


Query: 5   TLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCC--TWYCVECGE 64
           ++ LV+L+S   + +E CNP DK+ALL+IKK   +P  L+SW P  DCC  TW  V C  
Sbjct: 15  SIILVILVSLRTALSELCNPQDKQALLQIKKDLGNPTTLSSWLPTTDCCNRTWLGVLCDT 74

Query: 65  KSD--RIITIDI-GTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKF 124
            +   R+  +D+ G +     PIP ++ +LP+L  L +  + NLVGP+ PAIAKL  L +
Sbjct: 75  DTQTYRVNNLDLSGHNLPKPYPIPSSLANLPYLNFLYIGGINNLVGPIPPAIAKLTQLHY 134

Query: 125 LDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPI 184
           L ++ TN+SG++PDFLS +  L  +  ++N L+GTLP S+S LP LG +  D N+++G I
Sbjct: 135 LYITHTNVSGAIPDFLSQIKTLVTLDFSYNALSGTLPPSISSLPNLGGITFDGNRISGAI 194

Query: 185 PDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWK 244
           PDS+G        + +S N+L+GKIPP+   L+L  +DLSRN LEGDAS++FG+ K   K
Sbjct: 195 PDSYGSFSKLFTAMTISRNRLTGKIPPTFANLNLAFVDLSRNMLEGDASVLFGSDKNTKK 254

Query: 245 VDLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIP 304
           + L++N L+FD+ KV  S +L  LDL +N+I+G LP  +T+L  L S NVS+N LCG IP
Sbjct: 255 IHLAKNSLAFDLGKVGLSKNLNGLDLRNNRIYGTLPQGLTQLKFLQSLNVSFNNLCGEIP 314

Query: 305 QGGELQSFDVYSYFHNKCLCGAPLPSC 327
           QGG L+ FDV SY +NKCLCG+PLPSC
Sbjct: 315 QGGNLKRFDVSSYANNKCLCGSPLPSC 341

[Close]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL
Match: A0A6J1HMU3 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.5e-145
Identity = 250/320 (78.12%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   + FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY VEC E S RI
Sbjct: 13  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP VGDLPFLQ+L+L KLPNLVGP+QPAIAKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FGA +  WKVDLSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 253 EFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL
Match: A0A6J1EB58 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.5e-145
Identity = 249/320 (77.81%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   L FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY VEC E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP VGDLPFLQ+L+L KLPNLVGP+QPA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FG  +  WKVDLSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL
Match: A0A6J1HP52 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 232/320 (72.50%), Postives = 267/320 (83.44%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   + FTISFAE CNPNDKKALL IKKAFN+ Y  ASWK EEDCCTWY V+C E S RI
Sbjct: 17  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKAEEDCCTWYSVQCDENSHRI 76

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++ +  D+++SG IPP VG+LPFLQ L   KLPNLVGP+QPAIAKLHNLK LD+SWT I
Sbjct: 77  ISLTLAVDKQISGQIPPHVGELPFLQYLTFHKLPNLVGPIQPAIAKLHNLKHLDISWTKI 136

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L NL YI L+F+NL+G +P+SLSKLP L  LQLDR+KLTG IPDSFG  K
Sbjct: 137 SGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSYLQLDRSKLTGQIPDSFGDFK 196

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           AE+  L LSHNKLSGKIPPSL+++D  ++DLSRNKLEGDA M+FGAQKKAW VDLSRNLL
Sbjct: 197 AENFNLYLSHNKLSGKIPPSLSKVDFSAIDLSRNKLEGDAYMIFGAQKKAWNVDLSRNLL 256

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
           +FDMSKVVFST+LF LD+NHNKIFGGLPPQM+KLS L S NVSYNRLCG IP+G +L+ F
Sbjct: 257 AFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSYNRLCGQIPKGKKLKRF 316

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
            V SYFHNKCLCG PL SCK
Sbjct: 317 GVDSYFHNKCLCGKPLESCK 335

[Close]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL
Match: A0A5D3CYT3 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004960 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.4e-123
Identity = 218/325 (67.08%), Postives = 252/325 (77.54%), Query Frame = 0


Query: 4   FTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEK 63
           F +F       TISFAE CNPNDKK LL+IKKAFN+PY+L SW P+ DCC WYCV+C   
Sbjct: 14  FFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCDRT 73

Query: 64  SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVS 123
           + RII++ I  D+ L+G IPP VGDLPFLQTL+L KLPNL GP+QP IAKLHNLKFLD+S
Sbjct: 74  THRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHNLKFLDLS 133

Query: 124 WTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 183
           W  +SG +PD LSTL NL  + L+FN LTG +P+SLS+LP LG L+LDRN+LTG IPDSF
Sbjct: 134 WNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLTGQIPDSF 193

Query: 184 GYLKAESIV-LDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDL 243
           GY   E    L LSHN+LSGKIP SL+RL+  S+DLSRNKLEGDA M FGA K    +DL
Sbjct: 194 GYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANKTIQILDL 253

Query: 244 SRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGG 303
           SRNLL F++S+VVF  SL  LDLNHNK+FGGLPP MT+L  L   NVSYNRLCGPIP GG
Sbjct: 254 SRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTEL-KLQYLNVSYNRLCGPIPVGG 313

Query: 304 ELQSFDVYSYFHNKCLCGAPLPSCK 328
            LQSFD+YSYFHNKCLCGAPL  CK
Sbjct: 314 NLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL
Match: A0A1S3BU59 (polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.4e-123
Identity = 218/325 (67.08%), Postives = 252/325 (77.54%), Query Frame = 0


Query: 4   FTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEK 63
           F +F       TISFAE CNPNDKK LL+IKKAFN+PY+L SW P+ DCC WYCV+C   
Sbjct: 14  FFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCDRT 73

Query: 64  SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVS 123
           + RII++ I  D+ L+G IPP VGDLPFLQTL+L KLPNL GP+QP IAKLHNLKFLD+S
Sbjct: 74  THRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHNLKFLDLS 133

Query: 124 WTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 183
           W  +SG +PD LSTL NL  + L+FN LTG +P+SLS+LP LG L+LDRN+LTG IPDSF
Sbjct: 134 WNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLTGQIPDSF 193

Query: 184 GYLKAESIV-LDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDL 243
           GY   E    L LSHN+LSGKIP SL+RL+  S+DLSRNKLEGDA M FGA K    +DL
Sbjct: 194 GYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANKTIQILDL 253

Query: 244 SRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGG 303
           SRNLL F++S+VVF  SL  LDLNHNK+FGGLPP MT+L  L   NVSYNRLCGPIP GG
Sbjct: 254 SRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTEL-KLQYLNVSYNRLCGPIPVGG 313

Query: 304 ELQSFDVYSYFHNKCLCGAPLPSCK 328
            LQSFD+YSYFHNKCLCGAPL  CK
Sbjct: 314 NLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of Lcy04g004630 vs. NCBI nr
Match: XP_022966387.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 523.1 bits (1346), Expect = 1.7e-144
Identity = 250/320 (78.12%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   + FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY VEC E S RI
Sbjct: 13  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP VGDLPFLQ+L+L KLPNLVGP+QPAIAKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FGA +  WKVDLSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 253 EFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Lcy04g004630 vs. NCBI nr
Match: XP_022925061.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 523.1 bits (1346), Expect = 1.7e-144
Identity = 249/320 (77.81%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   L FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY VEC E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP VGDLPFLQ+L+L KLPNLVGP+QPA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FG  +  WKVDLSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Lcy04g004630 vs. NCBI nr
Match: KAG6595655.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 522.7 bits (1345), Expect = 2.3e-144
Identity = 249/320 (77.81%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   L FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY V C E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVHCDENSHRI 72

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP VGDLPFLQ+L+L KLPNLVGP+QPA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FGA +  WKVDLSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Lcy04g004630 vs. NCBI nr
Match: XP_023517556.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 3.3e-143
Identity = 246/320 (76.88%), Postives = 273/320 (85.31%), Query Frame = 0


Query: 8   LVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEKSDRI 67
           L   L FTISFAE CNPNDKKALL IKKAFN+ Y  ASWKPEEDCCTWY V+C E S RI
Sbjct: 17  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVDCDENSHRI 76

Query: 68  ITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVSWTNI 127
           I++DI TD E+SG IPP +GDLPFLQ+L+L KLPNLVGP+QP IAKLHNLK LD+SWTNI
Sbjct: 77  ISLDISTDNEISGQIPPYIGDLPFLQSLMLHKLPNLVGPIQPTIAKLHNLKHLDISWTNI 136

Query: 128 SGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFLS+L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 137 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPSLEFLQLDRSKLTGQIPYSFGDFK 196

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRNKLEGDASM+FG  +  WKVDLSRNLL
Sbjct: 197 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGEHRHTWKVDLSRNLL 256

Query: 248 SFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKL NLNSFNVSYNRLCG IP+GGELQ F
Sbjct: 257 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLGNLNSFNVSYNRLCGQIPKGGELQRF 316

Query: 308 DVYSYFHNKCLCGAPLPSCK 328
           DVYSYFHNKCLCGAPL SCK
Sbjct: 317 DVYSYFHNKCLCGAPLESCK 336

[Close]

BLAST of Lcy04g004630 vs. NCBI nr
Match: KAG6595656.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 486.5 bits (1251), Expect = 1.8e-133
Identity = 233/324 (71.91%), Postives = 272/324 (83.95%), Query Frame = 0


Query: 4   FTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGEK 63
           F+L L+ +  FTISFAE CNPNDKKALL IK+AFN+ Y  ASWKPEEDCCTWY V+C E 
Sbjct: 14  FSLLLIFIF-FTISFAELCNPNDKKALLNIKEAFNNAYYFASWKPEEDCCTWYSVQCDEI 73

Query: 64  SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLDVS 123
           S RII++ I  D+++SG IPP VGDLP+LQ L   KLPNLVGP+QPAIAKLHNLK LD+S
Sbjct: 74  SHRIISLTISVDKQISGQIPPHVGDLPYLQYLTFHKLPNLVGPIQPAIAKLHNLKHLDIS 133

Query: 124 WTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 183
           WT ISG++PDFLS+L NL YI L+F+NL+G +P+SLSKLP L +LQLDR+KLTG IPDSF
Sbjct: 134 WTKISGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSSLQLDRSKLTGQIPDSF 193

Query: 184 GYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVDLS 243
           G  KAE+  L LSHNKL+GKIPPSL+++D  ++DLSRNKLEGDA M+FGA KKAW VDLS
Sbjct: 194 GDFKAENFNLYLSHNKLTGKIPPSLSKVDFSAIDLSRNKLEGDAYMIFGAHKKAWNVDLS 253

Query: 244 RNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGE 303
           RNLL+FDMSKVVFST+LF LD+NHNKIFGGLPPQM+KLS L S NVSYNRLCG IP+G +
Sbjct: 254 RNLLAFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSYNRLCGQIPKGKK 313

Query: 304 LQSFDVYSYFHNKCLCGAPLPSCK 328
           L+ F V SYFHNKCLCG PL SCK
Sbjct: 314 LKRFGVDSYFHNKCLCGKPLESCK 335

[Close]

BLAST of Lcy04g004630 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 382.5 bits (981), Expect = 3.4e-106
Identity = 187/327 (57.19%), Postives = 239/327 (73.09%), Query Frame = 0


Query: 5   TLFLVLLLSF--TISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGE 64
           TL L+ L +F  T    + CN NDK  LL+IKK+ N+PY LASW P+ DCC+WYC+ECG+
Sbjct: 6   TLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGD 65

Query: 65  K--SDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFL 124
              + R+  + I +  ++SG IP  VGDLP+L+TLV RKL NL G +QP IAKL NL+ L
Sbjct: 66  ATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRML 125

Query: 125 DVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIP 184
            +SWTN++G +PDF+S L NLE++ L+FN+L+G++P+SLS LPK+ AL+L RNKLTG IP
Sbjct: 126 RLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIP 185

Query: 185 DSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKV 244
           +SFG        L LSHN+LSG IP SL  +D   +DLSRNKL+GDASM+FG+ K  W +
Sbjct: 186 ESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 245

Query: 245 DLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQ 304
           DLSRN+  FD+SKV    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP 
Sbjct: 246 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPT 305

Query: 305 GGELQSFDVYSYFHNKCLCGAPLPSCK 328
           GG+LQ+FD YSYFHNKCLCGAPL  CK
Sbjct: 306 GGKLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of Lcy04g004630 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 382.5 bits (981), Expect = 3.4e-106
Identity = 188/326 (57.67%), Postives = 241/326 (73.93%), Query Frame = 0


Query: 5   TLFLVL--LLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCTWYCVECGE 64
           TLFL+L  LL  T    + C+ +DK  LL+IKK+ N+PY LASW P+ DCC+WYC+ECG+
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 65  KS-DRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKFLD 124
            + +  +T  I  D E+SG IPP VGDLP+L +L+ RKL NL G +QP IAKL NL FL 
Sbjct: 66  ATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 125

Query: 125 VSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPD 184
           +SWTN++G VP+FLS L NLEYI L+FN+L+G++P+SLS L KL  L+L RNKLTGPIP+
Sbjct: 126 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 185

Query: 185 SFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWKVD 244
           SFG    +   L LSHN+LSG IP SL   D + +DLSRNKL+GDAS++FGA+K  W VD
Sbjct: 186 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 245

Query: 245 LSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQG 304
           +SRN+  FD+SKV  + +L +LD+NHN I G +P + +K +     NVSYNRLCG IP+G
Sbjct: 246 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKG 305

Query: 305 GELQSFDVYSYFHNKCLCGAPLPSCK 328
             +Q FD YS+FHNKCLCGAPLPSCK
Sbjct: 306 EYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Lcy04g004630 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.2e-80
Identity = 157/327 (48.01%), Postives = 213/327 (65.14%), Query Frame = 0


Query: 1   MHPFTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPYVLASWKPEEDCCT-WYCVE 60
           +H    F +L ++   S++  C  NDK ALL+IKKA  +P +L+SW P  DCCT W  VE
Sbjct: 5   VHLSIFFSILFITLPSSYS--CTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVE 64

Query: 61  CGEKSDRIITIDIGTDEELSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLHNLKF 120
           C   ++R +T    T  E+SG I   +GDL  L+TL    LP+L G +   I KL NL  
Sbjct: 65  C---TNRRVTGLSVTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNT 124

Query: 121 LDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPI 180
           L +  T++SG +PD++S L +L ++ L+FN  TG +P SLS++PKL A+Q++ NKLTG I
Sbjct: 125 LYLKHTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSI 184

Query: 181 PDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSLDLSRNKLEGDASMVFGAQKKAWK 240
           P+SFG        L LS+NKLSGKIP SL++ D  ++DLS N  EGDA M FG  K   +
Sbjct: 185 PNSFGSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVR 244

Query: 241 VDLSRNLLSFDMSKVVFSTSLFHLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIP 300
           VDLSRN+ +FD+ KV F+ S+  LDL+ N I+G +PP +TKL +L  FNVS N LCG IP
Sbjct: 245 VDLSRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIP 304

Query: 301 QGGELQSFDVYSYFHNKCLCGAPLPSC 327
            GG LQ+F+  ++ HN CLCG PL +C
Sbjct: 305 SGGLLQTFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of Lcy04g004630 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 119/362 (32.87%), Postives = 176/362 (48.62%), Query Frame = 0


Query: 3   PFTLFLVLLLSFTISFAERCNPNDKKALLEIKKAFNDPY--VLASWKPEEDCC-TWYCVE 62
           PF+  L ++    IS    C+P D+ AL   K + ++P   +  +W    DCC  WY + 
Sbjct: 8   PFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGIS 67

Query: 63  CGEKSDRIITIDIGTDEE------------LSGPIPPAVGDLPFLQTLVLRKLPNLVGPL 122
           C   S R+  I +  + E            +SG I PAV DL  L +LVL     + G +
Sbjct: 68  CDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGEI 127

Query: 123 QPAIAKLHNLKFLDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGA 182
            P I  L +L+ LD++   I+G +P  +  L  L  + L  N ++G +PASL+ L +L  
Sbjct: 128 PPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELKH 187

Query: 183 LQLDRNKLTGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLD-LFSLDLSRNKLEGD 242
           L+L  N +TG IP  FG LK  S VL L  N+L+G IP S++ ++ L  LDLS+N +EG 
Sbjct: 188 LELTENGITGVIPADFGSLKMLSRVL-LGRNELTGSIPESISGMERLADLDLSKNHIEGP 247

Query: 243 ASMVFGAQKKAWKV------------------------DLSRNLLSFDMSKVVFS-TSLF 302
                G  K    +                        +LSRN L   +  V  S T L 
Sbjct: 248 IPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYLV 307

Query: 303 HLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSFDVYSYFHNKCLCGA 324
            LDL+HN + G +P  ++    +   ++S+N+LCG IP G      +  S+  N+CLCG 
Sbjct: 308 SLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLCGG 367

[Close]

BLAST of Lcy04g004630 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.8e-45
Identity = 116/357 (32.49%), Postives = 174/357 (48.74%), Query Frame = 0


Query: 10  LLLSFTISFAERCNPNDKKALLEIKKAFNDPY--VLASWKPEEDCCTWYCVECGEKSDRI 69
           LLLS T+     C P+D+ ALLE +   N+PY  V  +WK  + C  WY V C   + R+
Sbjct: 18  LLLSTTV---HSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCDPNTRRV 77

Query: 70  ITIDIGTDEE------------LSGPIPPAVGDLPFLQTLVLRKLPNLVGPLQPAIAKLH 129
             I +  + E            ++G I P++  L  L  +++     + G +   I  L 
Sbjct: 78  AGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPSCIENLP 137

Query: 130 NLKFLDVSWTNISGSVPDFLSTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKL 189
            L+ LD+     SG +P  +  L  L+ + L  N+L G +P S+++L  L  L L  N +
Sbjct: 138 FLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLDLRNNNI 197

Query: 190 TGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRL-DLFSLDLSRNKLEGDASMVFGAQ 249
           +G IP   G LK  S VL LS NK+SG+IP SLTR+  L  L+LS N+L G     FG  
Sbjct: 198 SGVIPRDIGRLKMVSRVL-LSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIPASFGKM 257

Query: 250 KKAWKVDLSRNLLSFDMSKVVFSTSLFH------------------------LDLNHNKI 309
                ++L  NL+S  +   + ++S+ +                        LDL +N++
Sbjct: 258 SVLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLDLANNRL 317

Query: 310 FGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQSFDVYSYFHNKCLCGAPLPSCK 328
            G +P  +T  S +   +VS+N LCG IP G      D  S+ +N CLCG PL +CK
Sbjct: 318 QGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLGNCK 370

The following BLAST results are available for this feature:

BLAST of Lcy04g004630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05091	9.7e-114	62.20	Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1	[more]
A7PW81	3.0e-107	61.42	Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1	[more]
Q9M5J9	4.8e-105	57.19	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1	[more]
Q9M5J8	4.8e-105	57.67	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2	[more]
P35334	4.3e-85	47.71	Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1	[more]

BLAST of Lcy04g004630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMU3	8.5e-145	78.13	polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=...	[more]
A0A6J1EB58	8.5e-145	77.81	polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 P...	[more]
A0A6J1HP52	1.1e-133	72.50	polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=...	[more]
A0A5D3CYT3	1.4e-123	67.08	Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BU59	1.4e-123	67.08	polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV...	[more]

BLAST of Lcy04g004630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022966387.1	1.7e-144	78.13	polygalacturonase inhibitor-like [Cucurbita maxima]	[more]
XP_022925061.1	1.7e-144	77.81	polygalacturonase inhibitor-like [Cucurbita moschata]	[more]
KAG6595655.1	2.3e-144	77.81	Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023517556.1	3.3e-143	76.88	polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
KAG6595656.1	1.8e-133	71.91	Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lcy04g004630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06860.1	3.4e-106	57.19	polygalacturonase inhibiting protein 1	[more]
AT5G06870.1	3.4e-106	57.67	polygalacturonase inhibiting protein 2	[more]
AT3G12145.1	3.2e-80	48.01	Leucine-rich repeat (LRR) family protein	[more]
AT3G12610.1	2.8e-47	32.87	Leucine-rich repeat (LRR) family protein	[more]
AT5G12940.1	9.8e-45	32.49	Leucine-rich repeat (LRR) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001611	Leucine-rich repeat	PFAM	PF00560	LRR_1	coord: 192..209 e-value: 0.85 score: 10.4
IPR001611	Leucine-rich repeat	PFAM	PF13855	LRR_8	coord: 116..175 e-value: 5.6E-7 score: 29.2
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 21..327 e-value: 7.9E-87 score: 293.3
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 24..60 e-value: 2.1E-11 score: 43.9
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 6..327
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 6..327
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 21..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g004630.1	Lcy04g004630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0090353	polygalacturonase inhibitor activity
molecular_function	GO:0005515	protein binding